Biosensors and Bioelectronics最新文献

英文中文

Peroxynitrite and amyloid-β dual-activated near-infrared theranostic probe for oxidative stress monitoring in Alzheimer's disease

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-28 DOI: 10.1016/j.bios.2025.117201

Huanxin Xue , Xueli Wang , Qiuyan Jiang , Jiale Ma , Man Shing Wong

Amyloid-β (Aβ), the hallmark of Alzheimer's disease (AD), is known to induce reactive oxygen species, peroxynitrite (ONOO⁻) which plays a crucial role in the pathogenesis and progression of this incurable disease. However, the development of tools that can directly detect the presence and monitor the level of Aβ-induced ONOO⁻ remains a great challenge. We report herein the development of an Aβ and ONOO⁻ synergistically activated NIR fluorescent probe for highly selective imaging of Aβ-induced ONOO⁻ level in vivo. Importantly, this responsive probe exhibits not only synergistically strong enhancement of fluorescence at 655 nm upon reacting with ONOO⁻ in the presence of Aβ but also high sensitivity down to 13 nM with minimal interference. The strong Aβ binding and low cytotoxicity enable the probe to successfully apply for detecting and visualizing endogenous ONOO⁻ level induced by Aβ in AD cell model. Remarkably, this ONOO⁻-responsive probe can be applied effectively to detect, monitor, and distinguish varying ONOO⁻ levels induced by Aβ in different age groups of AD mice, in which cerebral ONOO⁻ level rises with increasing age of AD mice along with Aβ plaque accumulation. Furthermore, the potent neuroprotection against Aβ-induced toxicity and anti-Aβ aggregation effect of the ONOO⁻-reaction product of the probe offer an extra therapeutic advantage of this ONOO⁻-responsive probe. In essence, this multifunctional theranostic probe can serve as a highly sensitive and specific imaging tool for visualizing and monitoring of ONOO⁻ level in the presence of Aβ in vivo, thereby facilitating more accurate early diagnosis and therapy of AD.

{"title":"Peroxynitrite and amyloid-β dual-activated near-infrared theranostic probe for oxidative stress monitoring in Alzheimer's disease","authors":"Huanxin Xue , Xueli Wang , Qiuyan Jiang , Jiale Ma , Man Shing Wong","doi":"10.1016/j.bios.2025.117201","DOIUrl":"10.1016/j.bios.2025.117201","url":null,"abstract":"<div><div>Amyloid-β (Aβ), the hallmark of Alzheimer's disease (AD), is known to induce reactive oxygen species, peroxynitrite (ONOO<sup>−</sup>) which plays a crucial role in the pathogenesis and progression of this incurable disease. However, the development of tools that can directly detect the presence and monitor the level of Aβ-induced ONOO<sup>−</sup> remains a great challenge. We report herein the development of an Aβ and ONOO<sup>−</sup> synergistically activated NIR fluorescent probe for highly selective imaging of Aβ-induced ONOO<sup>−</sup> level <em>in vivo</em>. Importantly, this responsive probe exhibits not only synergistically strong enhancement of fluorescence at 655 nm upon reacting with ONOO<sup>−</sup> in the presence of Aβ but also high sensitivity down to 13 nM with minimal interference. The strong Aβ binding and low cytotoxicity enable the probe to successfully apply for detecting and visualizing endogenous ONOO<sup>−</sup> level induced by Aβ in AD cell model. Remarkably, this ONOO<sup>−</sup>-responsive probe can be applied effectively to detect, monitor, and distinguish varying ONOO<sup>−</sup> levels induced by Aβ in different age groups of AD mice, in which cerebral ONOO<sup>−</sup> level rises with increasing age of AD mice along with Aβ plaque accumulation. Furthermore, the potent neuroprotection against Aβ-induced toxicity and anti-Aβ aggregation effect of the ONOO<sup>−</sup>-reaction product of the probe offer an extra therapeutic advantage of this ONOO<sup>−</sup>-responsive probe. In essence, this multifunctional theranostic probe can serve as a highly sensitive and specific imaging tool for visualizing and monitoring of ONOO<sup>−</sup> level in the presence of Aβ <em>in vivo</em>, thereby facilitating more accurate early diagnosis and therapy of AD.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117201"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive SERS nanoprobe-based multiplexed digital sensing platform for the simultaneous quantification of Alzheimer's disease biomarkers

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-28 DOI: 10.1016/j.bios.2025.117216

Jae-Eul Shim , Young Jun Kim , Eunil Hahm , Jong-Ho Choe , Ahruem Baek , Ryeong Myeong Kim , Eun-Ah You

Alzheimer's disease (AD) is a severe neurodegenerative disease that requires early diagnosis to manage its progression. Although the simultaneous detection of multiple AD biomarkers is expected to facilitate early assessment of AD risk, the lack of multiplexed sensing platforms for accurately quantifying multiple AD biomarkers remains a challenge. Here, we present a multiplexed digital sensing platform based on bumpy core–shell (BCS) surface-enhanced Raman spectroscopy (SERS) nanoprobes for ultrasensitive, quantitative, and simultaneous detection of Aβ42 and Aβ40 as AD biomarkers, enabling the accurate determination of the Aβ42/Aβ40 ratio. We synthesized BCS SERS nanoprobes with distinct Raman reporters to generate unique, intense, and reproducible SERS signals, offering single-nanoparticle sensitivity and quantification capabilities. These nanoprobes were subsequently employed in SERS-based immunoassays combined with digital SERS analysis for multiplexed quantification. The proposed platform accurately and quantitatively detected Aβ42 and Aβ40 across a range of five orders of magnitude, with a limit of detection of 8.7× 10⁻¹⁷ g/mL (1.9 × 10⁻¹⁷ M) for Aβ42 and 1.0 × 10⁻¹⁵ g/mL (2.3 × 10⁻¹⁶ M) for Aβ40, surpassing the performance of conventional enzyme-linked immunosorbent assays. Based on the exclusive detection of Aβ42 and Aβ40 using distinct SERS nanoprobes, the proposed sensing platform can also accurately quantify Aβ42 and Aβ40 at clinically relevant levels in both cerebrospinal fluid and blood plasma. Therefore, this sensing platform can be used to accurately and reliably determine the Aβ42/Aβ40 ratio, thus serving as an effective tool for the early diagnosis of AD.

阿尔茨海默病（AD）是一种严重的神经退行性疾病，需要早期诊断以控制病情发展。虽然同时检测多种阿尔茨海默病生物标志物有望促进对阿尔茨海默病风险的早期评估，但缺乏可准确量化多种阿尔茨海默病生物标志物的多重传感平台仍是一项挑战。在这里，我们提出了一种基于凹凸核壳（BCS）表面增强拉曼光谱（SERS）纳米探针的多重数字传感平台，用于超灵敏、定量和同时检测作为AD生物标志物的Aβ42和Aβ40，从而准确测定Aβ42/Aβ40的比值。我们合成了具有独特拉曼报告器的 BCS SERS 纳米探针，以产生独特、强烈和可重现的 SERS 信号，提供单纳米粒子灵敏度和定量能力。这些纳米探针随后被用于基于 SERS 的免疫测定，并结合数字 SERS 分析进行多重定量。所提出的平台可在五个数量级的范围内准确定量检测 Aβ42 和 Aβ40，Aβ42 的检测限为 8.7×10-17 g/mL（1.9×10-17 M），Aβ40 的检测限为 1.0×10-15 g/mL（2.3×10-16 M），超过了传统的酶联免疫吸附测定法。基于使用不同的 SERS 纳米探针对 Aβ42 和 Aβ40 进行独家检测，所提出的传感平台还能在脑脊液和血浆中精确定量临床相关水平的 Aβ42 和 Aβ40。因此，该传感平台可用于准确可靠地测定 Aβ42/Aβ40 比值，从而成为早期诊断老年痴呆症的有效工具。

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引用次数: 0

Quantum dots for biosensing: Classification and applications 用于生物传感的量子点：分类与应用。

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-25 DOI: 10.1016/j.bios.2025.117180

Daniel Quesada-González , Arben Merkoçi

Quantum dots (QDs) are the smallest nanomaterials (2–10 nm), with unique optical and electronic properties. Thanks to these properties, QDs have been standing during the last years as signal tags for different applications, including bioimaging, fluorescent biosensors and electrochemical assays. In this review, we explore the current state-of-the art on these nanomaterials, differentiating them between semiconductor and carbon-based QDs. Also, the review focuses on their unique advantages as transducers in different biosensing platforms.

引用次数: 0

Monitoring of inflammatory preterm responses via myometrial cell based multimodal electrophysiological and optical biosensing platform

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-25 DOI: 10.1016/j.bios.2025.117197

Haote Han , Xia Ying , Qiaoqiao Chen , Jiaru Fang , Dongxin Xu , Xuelian Lyu , Jilin Zheng , Ling Zou , Qiong Luo , Ning Hu

Preterm birth (PTB) remains a leading cause of neonatal morbidity and mortality, with inflammation-induced PTB posing a significant challenge due to its complex pathophysiology. To address this, we developed an in vitro platform utilizing hTERT-immortalized human myometrial (hTERT-HM) cells integrated with a multielectrode array (MEA) biosensing system and optical calcium imaging. Compared to primary uterine myometrial cells, hTERT-HM cells exhibit superior reproducibility, high scalability, and convenient manipulation, facilitating the consistent and large-scale investigations. This advanced system facilitates simultaneous real-time monitoring of electrophysiological activity and intracellular calcium transient, providing detailed insights into uterine cell behavior during inflammatory PTB. Our study revealed that oxytocin (OT) induces regular contractions in hTERT-HM cells, and the synergistic effect of OT and lipopolysaccharide (LPS) disrupts electrophysiological patterns and calcium signaling, closely mimicking the pathophysiology of inflammation-induced PTB. Meanwhile, magnesium sulfate is validated to effectively suppress OT-induced calcium release and mitigate LPS-triggered irregular electrophysiological signals. By integrating advanced biosensing technologies and advantages of hTERT-HM cells, this platform offers a reliable, reproducible model to investigate the mechanisms of inflammation-driven PTB and further develop targeted therapeutic interventions.

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引用次数: 0

Dual-mode, regenerated DNA motor for simultaneous detection of viral gene fragments and diagnosis of infectious disease

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-22 DOI: 10.1016/j.bios.2025.117186

Wei Li , Shuaijing Wang , Minzhao Lin , Xueying Chen , Jiayue Li , Wanling Cui , Rui Wang

This study presents a dual-mode and regenerated DNA motor powered by exonuclease III (Exo III) for the simultaneous detection of viral gene fragments. The detection methodology is categorized into two distinct operational modes. The first mode emphasizes the simultaneous detection of two viral gene fragments from a specific virus. The presence of both genes triggers the operation of the DNA motor, generating a singular signal output. This mode operates on an “AND” logical mechanism, which enhances the precision of positive case identification. The second mode facilitates the simultaneous detection of three viral gene fragments from three different viruses within a single assay. The presence of these genes activates their respective motors, yielding distinct signal outputs. This mode supports the multiplex detection of three target genes, thereby aiding in the identification of previously uncharacterized viruses infecting patients and alleviating the logistical and financial burdens associated with multiple testing procedures. The detection limit in the “AND” logical mode is at the aM level, while in the multiplex mode, it reaches the fM level, facilitating the sensitive detection of viral gene fragments. The DNA motor can be regenerated by separating and reconstituting the utilized orbits, enabling its reuse for up to seven cycles in the “AND” logical mode and five cycles in the multiplex mode. Accurate diagnoses were achieved for patients exhibiting upper respiratory symptoms. Therefore, the proposed motor offers a novel and regenerative approach for viral gene fragments detection, demonstrating significant promise for application in the clinical diagnosis of viral infectious diseases.

{"title":"Dual-mode, regenerated DNA motor for simultaneous detection of viral gene fragments and diagnosis of infectious disease","authors":"Wei Li , Shuaijing Wang , Minzhao Lin , Xueying Chen , Jiayue Li , Wanling Cui , Rui Wang","doi":"10.1016/j.bios.2025.117186","DOIUrl":"10.1016/j.bios.2025.117186","url":null,"abstract":"<div><div>This study presents a dual-mode and regenerated DNA motor powered by exonuclease III (Exo III) for the simultaneous detection of viral gene fragments. The detection methodology is categorized into two distinct operational modes. The first mode emphasizes the simultaneous detection of two viral gene fragments from a specific virus. The presence of both genes triggers the operation of the DNA motor, generating a singular signal output. This mode operates on an “AND” logical mechanism, which enhances the precision of positive case identification. The second mode facilitates the simultaneous detection of three viral gene fragments from three different viruses within a single assay. The presence of these genes activates their respective motors, yielding distinct signal outputs. This mode supports the multiplex detection of three target genes, thereby aiding in the identification of previously uncharacterized viruses infecting patients and alleviating the logistical and financial burdens associated with multiple testing procedures. The detection limit in the “AND” logical mode is at the aM level, while in the multiplex mode, it reaches the fM level, facilitating the sensitive detection of viral gene fragments. The DNA motor can be regenerated by separating and reconstituting the utilized orbits, enabling its reuse for up to seven cycles in the “AND” logical mode and five cycles in the multiplex mode. Accurate diagnoses were achieved for patients exhibiting upper respiratory symptoms. Therefore, the proposed motor offers a novel and regenerative approach for viral gene fragments detection, demonstrating significant promise for application in the clinical diagnosis of viral infectious diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"273 ","pages":"Article 117186"},"PeriodicalIF":10.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High throughput cell stiffness measurement via multiplexed impedance sensors

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-22 DOI: 10.1016/j.bios.2025.117158

Norh Asmare , A K M Arifuzzman , Ningquan Wang , Mert Boya , Ruxiu Liu , A. Fatih Sarioglu

Since physiological and pathological events change the mechanical properties of cells, tools that rapidly quantify such changes at the single-cell level can advance the utility of cell mechanics as a label-free biomarker. We demonstrate the capability to probe the population-level elastic modulus and fluidity of MDA-MB-231 cells at a throughput of up to 50 cell/second within a portable microchip. Our sensing scheme adapts a code multiplexing scheme to implement a distributed network of sensors throughout the microchip, thereby compressing all sensing events into a single electrical output. To validate our approach, we prepared cell samples whose stiffnesses were manipulated with chemical agents. We confirmed the expected effect of the chemicals agreed with the stiffness measurements reported by our microchip. Such a low-cost electronic assay that rapidly measures mechanical properties enables previously infeasible studies to advance the science of mechanobiology.

引用次数: 0

Mitigating dithiothreitol interference to gold/thiol interface in electrochemical detection of cathepsin B activity toward multiplex protease analysis

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-22 DOI: 10.1016/j.bios.2025.117193

Bingun Habarakadage , Sabari Rajendran , Zhaoyang Ren , Morgan J. Anderson , Jessica Koehne , Lingaraju Gorla , Shunya Morita , Sara Wu , Duy H. Hua , Jun Li

Proteases are overexpressed at various stages of conditions such as cancers and thus can serve as biomarkers for disease diagnosis. Electrochemical techniques to detect the activity of extracellular proteases have gained attraction due to their multiplexing capability. Here we employ an electrochemical approach based on a 3 × 3 gold (Au) microelectrode array (MEA) functionalized with (2-aminoethyl)ferrocene (AEF) tagged specific peptide substrates to monitor cathepsin B (CB) protease activity. Cleavage of these peptide substrates by proteases leads to an exponential decay in the alternating current voltammetry (ACV) signal. The protease activity is represented by the inverse of the decay time constant (1/τ), which is equal to (k_cat/K_M)[CB] based on the heterogeneous Michaelis-Menton model. However, the thiol/Au chemisorption linking AEF-peptide to gold electrodes is susceptible to interference by the protease activation reagent dithiothreitol (DTT), causing the peptides to desorb from the Au surface during continuous ACV measurement. This induces a false signal decay, masking the protease activity and reducing the sensor sensitivity. To address this, DTT is removed after activating CB using centrifugal filtration while EDTA is incorporated to maintain the enzyme activity. This allows accurate CB proteolysis kinetics and clarifies the roles of EDTA and DTT in activation. The intrinsic substrate-dependent cleavage by CB to three different peptide substrates has been demonstrated with the MEA chip, showcasing the potential for rapid activity profiling of multiple proteases. The study highlights the importance of understanding the interference of active bioreagents to the thiol/Au interface in broad redox-tagged electrochemical biosensors.

{"title":"Mitigating dithiothreitol interference to gold/thiol interface in electrochemical detection of cathepsin B activity toward multiplex protease analysis","authors":"Bingun Habarakadage , Sabari Rajendran , Zhaoyang Ren , Morgan J. Anderson , Jessica Koehne , Lingaraju Gorla , Shunya Morita , Sara Wu , Duy H. Hua , Jun Li","doi":"10.1016/j.bios.2025.117193","DOIUrl":"10.1016/j.bios.2025.117193","url":null,"abstract":"<div><div>Proteases are overexpressed at various stages of conditions such as cancers and thus can serve as biomarkers for disease diagnosis. Electrochemical techniques to detect the activity of extracellular proteases have gained attraction due to their multiplexing capability. Here we employ an electrochemical approach based on a 3 × 3 gold (Au) microelectrode array (MEA) functionalized with (2-aminoethyl)ferrocene (AEF) tagged specific peptide substrates to monitor cathepsin B (CB) protease activity. Cleavage of these peptide substrates by proteases leads to an exponential decay in the alternating current voltammetry (ACV) signal. The protease activity is represented by the inverse of the decay time constant (1/τ), which is equal to (k<sub>cat</sub>/K<sub>M</sub>)[CB] based on the heterogeneous Michaelis-Menton model. However, the thiol/Au chemisorption linking AEF-peptide to gold electrodes is susceptible to interference by the protease activation reagent dithiothreitol (DTT), causing the peptides to desorb from the Au surface during continuous ACV measurement. This induces a false signal decay, masking the protease activity and reducing the sensor sensitivity. To address this, DTT is removed after activating CB using centrifugal filtration while EDTA is incorporated to maintain the enzyme activity. This allows accurate CB proteolysis kinetics and clarifies the roles of EDTA and DTT in activation. The intrinsic substrate-dependent cleavage by CB to three different peptide substrates has been demonstrated with the MEA chip, showcasing the potential for rapid activity profiling of multiple proteases. The study highlights the importance of understanding the interference of active bioreagents to the thiol/Au interface in broad redox-tagged electrochemical biosensors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"273 ","pages":"Article 117193"},"PeriodicalIF":10.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction”[Biosensors and Bioelectronics 272 (2025)/117102]

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-22 DOI: 10.1016/j.bios.2025.117151

Qianqian Li , Huijuan Liu , Xiangjun Du , Yafan Xie , Yanwei Chen , Juhui Qiu , Yiqin Gao , Qin Peng

引用次数: 0

ECoGScope: An integrated platform for real-time Electrophysiology and fluorescence imaging

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-22 DOI: 10.1016/j.bios.2025.117196

Jonghee Eun , Jeongseop Kim , Tae-Eun Kim , Ja Wook Koo , Namsun Chou

In this study, we present ECoGScope, a versatile neural interface platform designed to integrate multiple functions for advancing neural network research. ECoGScope combines an electrocorticography (ECoG) electrode array with a commercial microendoscope, enabling simultaneous recording of ECoG signals and fluorescence imaging. The electrode array, constructed from highly flexible and transparent polymers, ensures conformal contact with the brain surface, allowing unobstructed optical monitoring of neural activity alongside electrophysiological recordings. A key innovation is the compact connection module, which securely integrates the ECoG array and microendoscope while minimizing interference with animal behavior. The device was successfully tested in the visual, somatosensory, and frontal cortex, demonstrating its capability for simultaneous electrophysiological and fluorescent measurements. These results highlight the potential of the ECoGScope platform to advance the development of multifunctional tools for studying brain function and addressing neurological disorders.

{"title":"ECoGScope: An integrated platform for real-time Electrophysiology and fluorescence imaging","authors":"Jonghee Eun , Jeongseop Kim , Tae-Eun Kim , Ja Wook Koo , Namsun Chou","doi":"10.1016/j.bios.2025.117196","DOIUrl":"10.1016/j.bios.2025.117196","url":null,"abstract":"<div><div>In this study, we present ECoGScope, a versatile neural interface platform designed to integrate multiple functions for advancing neural network research. ECoGScope combines an electrocorticography (ECoG) electrode array with a commercial microendoscope, enabling simultaneous recording of ECoG signals and fluorescence imaging. The electrode array, constructed from highly flexible and transparent polymers, ensures conformal contact with the brain surface, allowing unobstructed optical monitoring of neural activity alongside electrophysiological recordings. A key innovation is the compact connection module, which securely integrates the ECoG array and microendoscope while minimizing interference with animal behavior. The device was successfully tested in the visual, somatosensory, and frontal cortex, demonstrating its capability for simultaneous electrophysiological and fluorescent measurements. These results highlight the potential of the ECoGScope platform to advance the development of multifunctional tools for studying brain function and addressing neurological disorders.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"274 ","pages":"Article 117196"},"PeriodicalIF":10.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CuO2@SiO2 nanoparticle assisted click reaction-mediated magnetic relaxation biosensor for rapid detection of Salmonella in food

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-01-21 DOI: 10.1016/j.bios.2025.117188

Junpeng Zhao , Rui Chen , Aimin Ma , Yongzhen Dong , Minjie Han , Xuezhi Yu , Yiping Chen

Foodborne pathogens seriously threaten people's life and well-being. In this study, we developed a novel magnetic relaxation time (PCuMRS) biosensor by integrating phage, differential magnetic separation technology, and copper catalyzed click reaction to enable rapid and sensitive detection of viable Salmonella typhimurium (S. typhimurium) in food within 80 min. This assay utilized phage as the recognition element to accurately differentiate between viable and nonviable S. typhimurium. Initially, we prepared a complex of magnetic nanoparticles (MNPs) and phage to efficiently capture viable S. typhimurium. We synthesized CuO₂@SiO₂-phage nanoparticles loaded with numerous Cu²⁺ ions to transform the concentration of S. typhimurium into a corresponding concentration of copper ions, which then modulate the click reaction between magnetic nanoparticles of varying sizes, leading to changes in both the number of small magnetic nanoparticles and magnetic signals. Based on this principle, we established a linear relationship (10²–10⁷ CFU/mL) between the concentration of S. typhimurium and the changes in magnetic signal, with a limit of quantification of 80 CFU/mL. Furthermore, the standard recovery rate and coefficient of variation of the sensor are 93.68%–100.36% and 0.59%–4.76%, respectively. The PCuMRS biosensor demonstrates outstanding sensitivity and a short detection time, making it a rapid, sensitive, and accurate method for identifying foodborne pathogens such as S. typhimurium, which has potential for applications in various other fields.

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