Biosensors and Bioelectronics最新文献_第3页

Biotin-specific molecularly imprinted polymers as a biomimetic test line in lateral flow assays 生物素特异性分子印迹聚合物作为横向流动分析的仿生测试线

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-27 DOI: 10.1016/j.bios.2026.118415

Jennifer Marfà , Anaixis del Valle , Rafael Rovatti Pupin , Bàrbara Baro , Quique Bassat , Maria Del Pilar Taboada Sotomayor , María Isabel Pividori

Lateral flow assays are widely used for rapid diagnostics at the point of care. However, their dependence on biological receptors limits their stability, scalability, and cost-effectiveness. This work demonstrates the use of biotin-specific molecularly imprinted polymers (biotin-MIPs) as synthetic recognition elements in nucleic acid lateral flow (NALF) assays. The biotin-MIPs were synthesized, structurally characterized, and integrated into the nitrocellulose membrane as a test line. Their selective binding affinity was first validated using biotinylated horseradish peroxidase as a model analyte. The platform was then evaluated for the detection of double-tagged PCR amplicons from Escherichia coli labeled with biotin and digoxigenin, achieving a visual detection limit of 2 ng mL⁻¹ and a limit of detection of 1.8 ng mL⁻¹, with no detectable signal in negative controls. Clinical feasibility was further assessed retrospectively using swab specimens collected during routine third-trimester screening (35–37 weeks of gestation) for Group B Streptococcus, a major cause of neonatal sepsis. In this proof-of-concept study, the MIP-based NALF assay showed complete qualitative agreement with the qPCR reference classification and the gold standard microbiological culture, demonstrating the compatibility of this approach with battery-operated portable PCR amplification. Unlike biological receptors, MIPs offer robustness, long-term stability at room temperature, and animal-free scalable production. These features position the MIP-based NALF platform as a cost-effective alternative to antibody-based tests and a promising foundation for next-generation lateral flow diagnostics for the detection of communicable diseases at the point of care.

横向流动测定法广泛用于护理点的快速诊断。然而，它们对生物受体的依赖限制了它们的稳定性、可扩展性和成本效益。这项工作证明了生物素特异性分子印迹聚合物（生物素- mips）作为合成识别元件在核酸侧流（naff）检测中的应用。合成了生物素- mips，对其进行了结构表征，并将其整合到硝化纤维素膜中作为测试线。它们的选择性结合亲和力首先用生物素化的辣根过氧化物酶作为模型分析物进行验证。然后评估该平台对生物素和地高辛标记的大肠杆菌双标记PCR扩增子的检测能力，达到2 ng mL−1的视觉检测限和1.8 ng mL−1的检测限，阴性对照中没有检测到信号。临床可行性进一步回顾性评估，使用常规妊娠晚期筛查（妊娠35-37周）收集的拭子标本检测B组链球菌，B组链球菌是新生儿败血症的主要原因。在这项概念验证研究中，基于mip的nff分析与qPCR参考分类和金标准微生物培养在定性上完全一致，证明了该方法与电池操作的便携式PCR扩增的兼容性。与生物受体不同，mip具有稳健性，在室温下的长期稳定性，以及无动物的可扩展生产。这些特点使基于mip的nff平台成为基于抗体的测试的一种具有成本效益的替代方案，并为在护理点检测传染病的下一代侧流诊断奠定了有希望的基础。

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引用次数: 0

Dual-strain engineering in Pd metallene for ultrasensitive tetracycline electrochemical biosensing 超灵敏四环素电化学生物传感用金属钯双应变工程

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-27 DOI: 10.1016/j.bios.2026.118443

Yi Wen , Yinan Dong , Xuejie Shen , Xingyu Luo , Ziyi Wang , Ruirui Guo , Zhituo Geng , Peng Li , Yongping Huang , Xinyu Zhu , Jiatong Liu , Wangyang Fu , Zehui Li , Xiaoyan Zhang

Precise modulation of strain and electronic structure via atomic-level Mo and Cr co-doping in Pd metallene (MoCr-Pdene) significantly enhances electrocatalytic tetracycline (TC) sensing. Conventional Pd-based sensors suffer from weak TC affinity and sluggish kinetics due to surface inertness and biofouling. MoCr-Pdene overcomes these limitations through synergistic dual-strain effects, including compressive strain (from Cr) stabilizing the lattice and tensile strain (from Mo) redistributing surface charge. This strain-charge synergy shortens the Pd-O adsorption distance (1.82 Å), enhances adsorption energy (0.86 eV), and accelerates electron transfer, enabling an ultra-low detection limit of 27 nM, which represents ten-fold improvement over commercial Pd/C. The co-doped metallene exhibits exceptional stability (retaining 80 % of its signal after 120 days) and anti-biofouling capability in matrices. Importantly, MoCr-Pdene serves as a dual-functional platform for clinical diagnostics and environmental monitoring, quantitatively assessing TC biodegradation by black soldier fly larvae (BSFL) and gut microbiota (degradation rates: 97.96 % in frass, 90.67 % in BSFL). These results pioneer atomic-scale strain-electronic engineering for next-generation antibiotic sensing and sustainable bioremediation.

通过原子水平Mo和Cr共掺杂金属钯（MoCr-Pdene），精确调制应变和电子结构，显著增强电催化四环素（TC）传感。由于表面惰性和生物污染，传统的基于pd的传感器具有较弱的TC亲和力和缓慢的动力学。MoCr-Pdene通过协同的双应变效应克服了这些限制，包括压缩应变（来自Cr）稳定晶格和拉伸应变（来自Mo）重新分配表面电荷。这种菌株-电荷协同作用缩短了Pd- o的吸附距离（1.82 Å），提高了吸附能（0.86 eV），并加速了电子转移，实现了27 nM的超低检测限，比商用Pd/C提高了10倍。共掺杂的金属烯在基质中表现出优异的稳定性（120天后仍能保持80% %的信号）和抗生物污染能力。重要的是，MoCr-Pdene作为临床诊断和环境监测的双重功能平台，定量评估黑兵蝇幼虫（BSFL）和肠道微生物群对TC的生物降解（降解率：在草中为97.96 %，在BSFL中为90.67 %）。这些结果开创了下一代抗生素传感和可持续生物修复的原子尺度菌株电子工程。

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引用次数: 0

An ultrasensitive FEN1-aided LCR-electrochemical biosensor enables detection of BCR/ABL1 fusion transcripts in clinical samples for early diagnosis and minimal residual monitoring of CML 一种超灵敏的fen1辅助lcr -电化学生物传感器可以检测临床样品中的BCR/ABL1融合转录物，用于CML的早期诊断和最小残留监测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-27 DOI: 10.1016/j.bios.2026.118442

Liang-Yong Yang , Guang-Hui Mei , Xin-Ru Lin , Cai-Hong Wang , Rui-Long Lan , Chen-Liu Ye , Yan-Fang Xu , Xin-Hua Lin , Jin-Yuan Chen

Ligase chain reaction (LCR) is a promising nucleic acid amplification technique widely applied in molecular diagnosis. However, a critical limitation of LCR is target-independent amplification, primarily caused by the blunt-end ligation activity of thermophilic DNA ligases, which results in false positives and compromises sensitivity. To overcome this challenge, we developed a thermostable flap endonuclease 1 (FEN1)-aided LCR (FALCR) employing DNA probes with non-phosphorylated sticky ends. In each amplification cycle, FEN1 selectively cleaves the 5′ DNA flap of the downstream probe in the presence of a DNA template, generating 5′-phosphorylated DNA nicks that enable ligation. In the absence of a DNA template, the non-phosphorylated sticky ends of the probes prevent ligation, effectively eliminating nonspecific amplification. We further integrated FALCR with a magnetic platform-based electrochemical biosensor (FA-eLCR), achieving ultrasensitive detection of DNA targets with a limit of detection (LOD) of 0.1 aM and a dynamic range spanning 10 aM to 10 fM. Furthermore, the designing of a duplex FALCR enabled the detection of BCR/ABL1^p210 comprising e13a2/e14a2/both isoforms in a single assay. Finally, the duplex FA-eLCR successfully detected the BCR/ABL1^p210 transcripts in patients with chronic myeloid leukemia (CML) and monitored the change of transcripts during treatment, with 100 % concordance to reverse transcription-quantitative polymerase chain reaction, highlighting its high potential in the early diagnosis and minimal residual disease (MRD) monitoring of CML. This work presents a novel strategy to address nonspecific amplification in LCR and introduces a cost-effective, highly sensitive platform for molecular diagnosis in clinical setting.

连接酶链反应（LCR）是一种具有广泛应用前景的核酸扩增技术。然而，LCR的一个关键限制是不依赖靶标的扩增，这主要是由嗜热DNA连接酶的钝端连接活性引起的，这会导致假阳性和降低灵敏度。为了克服这一挑战，我们利用具有非磷酸化粘性末端的DNA探针开发了一种耐热皮瓣内切酶1 （FEN1）辅助LCR （FALCR）。在每个扩增周期中，FEN1在DNA模板存在的情况下选择性地切割下游探针的5 ‘ DNA瓣，产生5 ’磷酸化的DNA缺口，从而实现连接。在没有DNA模板的情况下，探针的非磷酸化粘性末端防止结扎，有效地消除了非特异性扩增。我们进一步将FALCR与基于磁性平台的电化学生物传感器（FA-eLCR）集成在一起，实现了对DNA靶标的超灵敏检测，检测限（LOD）为0.1 aM，动态范围为10 aM至10 fM。此外，双相FALCR的设计使BCR/ABL1p210包含e13a2/e14a2/两种亚型的检测成为可能。最后，双链FA-eLCR成功检测了慢性髓性白血病（CML）患者的BCR/ABL1p210转录本，并监测了治疗过程中转录本的变化，与逆转录-定量聚合酶链反应的一致性为100 %，突出了其在CML早期诊断和微小残留病（MRD）监测中的巨大潜力。这项工作提出了一种解决LCR非特异性扩增的新策略，并引入了一种具有成本效益，高度敏感的临床分子诊断平台。

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引用次数: 0

Dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering biosensor combined with a smartphone for simultaneous detection of total and live target bacteria 双CRISPR/ cas驱动的无扩增表面增强拉曼散射生物传感器与智能手机相结合，用于同时检测总细菌和活目标细菌

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-27 DOI: 10.1016/j.bios.2026.118446

Ruibao Jiao , Qun Ni , Ruirui Zhao , Xiaofan Zhu , Lixia Wang , Jingran Jiao , Han Jiang , Qian Wu , Shuang Yao , Li Yao , Kaiyong Liu , Panzhu Qin

Simultaneous detection of total and live counts of target bacteria is significant but challenging. To address this challenge, here we proposed a dual CRISPR/Cas-driven amplification-free surface-enhanced Raman scattering (SERS) biosensor, termed cc-SERS. The biosensor was constructed based on the property that DNA remains stable while some RNA degrades rapidly after bacterial death. Briefly, the target DNA and RNA from live bacteria could activate both CRISPR/Cas12a and CRISPR/Cas13a, while dead bacteria could only activate CRISPR/Cas12a through the target DNA. In the absence of the target bacteria, neither CRISPR/Cas12a nor CRISPR/Cas13a could be activated. Therefore, the characteristic Raman signal at 1079 cm⁻¹ generated by the target DNA-activated CRISPR/Cas12a indicated the presence of the target bacteria (the sum of dead and live), while the characteristic Raman signal at 593 cm⁻¹ produced by the target RNA-activated CRISPR/Cas13a indicated the presence of the live target bacteria. With this unique signaling pattern, the biosensor is capable of detecting both total and live target bacteria in a single tube with a detection limit of ∼10 CFU/mL. The introduction of a rapid pre-processing procedure and a smartphone-assisted portable Raman spectrometer enabled the entire process to be completed in the field within 45 min. Thanks to the excellent programmability of CRISPR/Cas systems, the biosensor has been successfully applied to the detection of Staphylococcus aureus and Cronobacter sakazakii, respectively. As a proof-of-concept, this work opens a promising avenue for the simultaneous detection of total and live target bacteria.

同时检测目标细菌的总数和活计数是重要的，但具有挑战性。为了解决这一挑战，我们提出了一种双CRISPR/ cas驱动的无扩增表面增强拉曼散射（SERS）生物传感器，称为cc-SERS。该生物传感器是基于细菌死亡后DNA保持稳定而一些RNA迅速降解的特性而构建的。简而言之，活菌的靶DNA和RNA可以同时激活CRISPR/Cas12a和CRISPR/Cas13a，而死菌只能通过靶DNA激活CRISPR/Cas12a。在没有目标细菌的情况下，CRISPR/Cas12a和CRISPR/Cas13a都不能被激活。因此，靶dna激活的CRISPR/Cas12a产生的1079 cm−1处的特征拉曼信号表明存在目标菌（死菌和活菌之和），而靶rna激活的CRISPR/Cas13a产生的593 cm−1处的特征拉曼信号表明存在活的目标菌。凭借这种独特的信号模式，该生物传感器能够在单管中检测总目标细菌和活目标细菌，检测限为~ 10 CFU/mL。快速预处理程序和智能手机辅助便携式拉曼光谱仪的引入使整个过程在45 min内完成。由于CRISPR/Cas系统出色的可编程性，该生物传感器已分别成功应用于金黄色葡萄球菌和阪崎克罗诺杆菌的检测。作为概念验证，这项工作为同时检测总目标细菌和活目标细菌开辟了一条有希望的途径。

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引用次数: 0

Antibiotic-induced autocatalytic DNA circuit for enrofloxacin detection based on triple signal amplification strategy. 基于三重信号放大策略的抗生素诱导自催化DNA电路检测恩诺沙星。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-27 DOI: 10.1016/j.bios.2026.118448

Junhua Chen, Gu Shi, Chong Yan

Using hairpin DNA as the sensing probes and aptamer sequence as the recognition probe, we have successfully constructed an autocatalytic DNA circuit biosensor for enrofloxacin (ENR) detection with high sensitivity and selectivity. The antibiotic-induced release of the trigger DNA (T) can initiate the cross-hybridization of hairpin probes to form three-way DNA junction products, in which the originally split T fragments in hairpin probes can be pulled together to form the intact T sequence. Both the released T and the newly regenerated T can be reused to speed up the generation of the three-way DNA junction. In the products, intact Mg²⁺-dependent DNAzyme sequences will be generated at two arms of the DNA junctions, which can cleave the FAM and BHQ labeled substrate hairpin probe, leading to the release of another T. Through the triple signal amplification strategy in the autocatalytic DNA circuit, we can get an exponentially amplified signal for ENR detection. The DNA circuit biosensor is ultrasensitive with a detection limit of 25.8 fM. The sensing system is robust and has been applied to the detection of ENR in real fish and water samples with good reliability and accuracy. With the advantages of enzyme-free format, highly efficient signal amplification efficiency, and good robustness in complex samples, we anticipate that this biosensor will be a powerful tool for antibiotic residue monitoring in environmental and food samples.

以发夹DNA为传感探针，适体序列为识别探针，成功构建了一种具有高灵敏度和选择性的自催化DNA电路生物传感器，用于恩诺沙星（ENR）检测。抗生素诱导的触发DNA (T)的释放可以启动发夹探针的交叉杂交，形成三向DNA连接产物，发夹探针中原本分裂的T片段可以被拉在一起，形成完整的T序列。释放的T和新再生的T都可以重复使用，以加速三向DNA连接的生成。在产品中，完整的Mg2+依赖性DNAzyme序列将在DNA连接处的两条臂上生成，该序列可以切割FAM和BHQ标记的底物发夹探针，从而释放另一个t。通过自催化DNA电路中的三重信号放大策略，我们可以获得指数级放大的信号，用于ENR检测。DNA电路生物传感器具有超灵敏的检测限为25.8 fM。该传感系统具有较强的鲁棒性，并已应用于实际鱼和水样中ENR的检测，具有较好的可靠性和准确性。该传感器具有无酶格式、高效的信号放大效率和对复杂样品的鲁棒性等优点，有望成为环境和食品样品中抗生素残留监测的有力工具。

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引用次数: 0

Roll-to-roll printed in-pad biosensors for colorimetric detection of key biomarkers in artificial vaginal fluid 用于人工阴道液中关键生物标志物比色检测的卷对卷印刷衬垫生物传感器

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-26 DOI: 10.1016/j.bios.2026.118433

Ziheng Wang , Semin Kwon , Tianhao Yu , Yumin Dai , Young L. Kim , Chi Hwan Lee

Gynecological conditions often lack accessible, non-invasive diagnostic tools, leading to delayed detection and treatment, especially in resource-limited settings. Vaginal discharge and menstrual blood offer abundant but underutilized biomarkers for diagnosing gynecological disorders. Although sanitary pads represent an ideal platform for continuous fluid monitoring, direct high-throughput fabrication of sensors onto commercial pads remains challenging. Here, we introduce a roll-to-roll (R2R) wax-printing method optimized for large-scale production of fluidic colorimetric sensor patches designed for integration into sanitary pads. Utilizing specially formulated wax inks, this scalable approach enables consistent, cost-effective sensor fabrication. The sensor patches were functionalized with colorimetric reagents, incorporating enzymatic sensing elements for uric acid and glucose and physicochemical indicators for pH and moisture. This configuration enabled robust multi-analyte detection with clear and interpretable visual outputs, observable directly by the naked eye or via smartphone-based analysis. Validation in artificial vaginal fluid (AVF) confirmed reliable sensor performance, underscoring a practical and scalable platform with potential to advance proactive women's health monitoring in both home-based and point-of-care settings.

妇科疾病往往缺乏可获得的非侵入性诊断工具，导致检测和治疗延迟，特别是在资源有限的环境中。阴道分泌物和经血为诊断妇科疾病提供了丰富但未充分利用的生物标志物。尽管卫生巾是连续流体监测的理想平台，但在商业卫生巾上直接制造高通量传感器仍然具有挑战性。在这里，我们介绍了一种卷对卷（R2R）蜡印方法，该方法针对大规模生产设计用于集成到卫生巾中的流体比色传感器贴片进行了优化。利用特殊配方的蜡油墨，这种可扩展的方法可以实现一致的，具有成本效益的传感器制造。用比色试剂对传感器贴片进行功能化，包括尿酸和葡萄糖的酶敏元件以及pH和水分的理化指标。这种配置实现了强大的多分析物检测，具有清晰和可解释的视觉输出，可直接通过肉眼或基于智能手机的分析观察到。在人工阴道液（AVF）中的验证证实了传感器的可靠性能，强调了一个实用且可扩展的平台，具有在家庭和护理点环境中推进主动妇女健康监测的潜力。

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引用次数: 0

Molecular-recognition architectures and emerging probe strategies for neurotransmitter chemical sensing. 神经递质化学传感的分子识别结构和新兴探针策略。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-26 DOI: 10.1016/j.bios.2026.118445

Jiaxue Wu, Yifan Da, Lindong Xu, Runqiao Yang, Hanshu Li, Xiaoya Zhou, Yang Tian, Yi Zhou

Monitoring neurotransmitter dynamics in the brain with high spatial and temporal fidelity is essential for understanding neural circuit function and dysfunction. Although detection technologies have advanced, the molecular recognition elements that initiate sensing still largely define selectivity, speed, and in vivo performance. Here we focus on rapidly evolving molecular-recognition architectures for small-molecule neurotransmitter probes. Moving beyond simple lock-and-key motifs, we compare receptor-inspired binding sites, reaction-driven chemistries, conformational switches, supramolecular host-guest complexes, and engineered proteins through four performance criteria: molecular selectivity, response speed, signal-reporting mechanisms, and in vivo stability. We also highlight how these architectures interface with optical and electrochemical readouts to enable real-time measurements in complex tissue. By framing recent advances within a common structure-performance landscape, this Perspective provides a chemistry-centric guide for designing next-generation molecular tools to interrogate neurotransmitter signaling in the brain.

以高时空保真度监测大脑中的神经递质动力学是理解神经回路功能和功能障碍的必要条件。尽管检测技术已经进步，但启动传感的分子识别元件仍然在很大程度上决定了选择性、速度和体内性能。在这里，我们专注于小分子神经递质探针快速发展的分子识别架构。除了简单的锁键基序，我们通过四个性能标准比较了受体激发的结合位点、反应驱动的化学反应、构象开关、超分子宿主-客体复合物和工程蛋白：分子选择性、反应速度、信号报告机制和体内稳定性。我们还强调了这些结构如何与光学和电化学读数接口，以实现复杂组织的实时测量。通过在一个共同的结构-性能景观框架内的最新进展，本观点为设计下一代分子工具来询问大脑中的神经递质信号提供了一个以化学为中心的指南。

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引用次数: 0

Single-atom co anchored UiO-66 to boost electrochemiluminescence of MoS2 for bioanalysis 单原子共锚定UiO-66增强MoS2电化学发光用于生物分析

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-24 DOI: 10.1016/j.bios.2026.118432

Jinwen Zhao , Guomin Yang , Yifan Ruan , Ruo Yuan , Shihong Chen

Single-atom catalysts (SACs) hold significant promise for improving the electrochemiluminescence (ECL) efficiency. However, the preparation of SACs reported in the ECL field usually relies on carbon-nitrogen-based materials as substrates and requires high-temperature pyrolysis (>800 °C), suffering from the limitation in the anchoring materials and the deficiency of insufficient active site density caused by high-temperature calcination. This work developed zirconium-based metal-organic framework UiO-66 as the carrier to successively anchor the Co single-atom (Co_SA) and the ECL emitter MoS₂ under mild conditions (not exceeding 200 °C). Co_SA not only can optimize the electronic structure of MoS₂ to effectively promote the formation of Co(IV)=O, but also can act as the co-reactant accelerator to efficiently catalyze the reduction of coreactant S₂O₈²⁻. Meanwhile, the Mo-O-Zr bimetallic sites formed between UiO-66 and MoS₂ can enhance the charge carrier mobility within MoS₂. As a result, the ECL emission of MoS₂ at −1.6 V was remarkably boosted when using step pulse as the electrochemical method. The synthesized MoS₂@Co_SA/UiO-66 composite integrated an aptamer recognition-driven cascaded polymerase amplification to achieve ultrasensitive ECL detection of p-Tau as crucial biomarker for Alzheimer's disease (AD). Co_SA/UiO-66 exhibits broad application prospects in improving ECL efficiency. MoS₂@Co_SA/UiO-66 builds a highly sensitive ECL sensing platform for detecting the biomarkers of AD and other diseases.

单原子催化剂（SACs）在提高电化学发光效率方面具有重要的前景。然而，ECL领域报道的SACs的制备通常依赖于碳氮基材料作为底物，并且需要高温热解（>800 °C），这受到锚定材料的限制以及高温煅烧导致活性位点密度不足的缺点。本工作开发了锆基金属有机骨架uuo -66作为载体，在温和条件下（不超过200 °C）连续锚定Co单原子（CoSA）和ECL发射极MoS2。CoSA不仅可以优化MoS2的电子结构，有效促进Co(IV)=O的形成，还可以作为助反应物促进剂，有效催化助反应物S2O82−的还原。同时，在UiO-66与MoS2之间形成的Mo-O-Zr双金属位可以提高MoS2内部载流子的迁移率。结果表明，在−1.6 V条件下，采用步进脉冲的电化学方法可以显著提高MoS2的ECL发射量。合成的MoS2@CoSA/UiO-66复合物整合了适体识别驱动的级联聚合酶扩增，实现了p-Tau作为阿尔茨海默病（AD）关键生物标志物的超灵敏ECL检测。CoSA/UiO-66在提高ECL效率方面具有广阔的应用前景。MoS2@CoSA/UiO-66构建了一个高灵敏度的ECL传感平台，用于检测AD等疾病的生物标志物。

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引用次数: 0

A novel self-powered electrochemical sensor based on zinc-air battery for thrombin detection 一种基于锌-空气电池的自供电电化学凝血酶检测传感器

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-24 DOI: 10.1016/j.bios.2026.118429

Zhengchun Sun , Weili Zhang , Junhong Liu , Shina Du , Yuebo Wang , Zhongfang Li , Xueliang Niu , Wei Sun

As an emerging sensing mode, self-powered electrochemical sensor (SPES) has gained much attention for its advantages of without external power supply and portable detection. However, developing SPES with enhanced output signals and anti-influence characteristics is still a challenge. To address these issues, herein, a novel zinc-air battery (ZAB)-based SPES with aptamer as a specific recognition element was constructed. In this case, bimetallic Fe/Ni and N co-doping carbon and in-situ grown carbon nanotubes (Fe-Ni/N-C/CNT) was synthesized by mixing Fe, Ni precursors with previously prepared ZIF-8. Under neutral conditions, Fe-Ni/N-C/CNT exhibits a high half-wave potential of 0.831 V (vs. RHE) and the ZAB assembled with Fe-Ni/N-C/CNT as an air cathode generates a considerable maximum peak power density (P_max) of 84.1 mW cm⁻². As a concept, thrombin (TB) was selected as a detection target, a “signal-off” SPES based on the interfacial steric hindrance effect generated by aptamers structure bending while capturing the target was constructed with P_max as a sensing signal. P_max values of the prepared ZAB-based SPES show a good linear relationship with logarithm of TB concentrations, accompanied by a linear range from 2 × 10⁻⁴ nM to 2 × 10⁻² nM, and a detection limit of 66.7 fM (LOD). The constructed ZAB-based SPES shows good anti-interference ability, stability, and reproducibility, making it feasible and efficient in practical applications. Furthermore, this SPES strategy displays a promising generalizability for other targets detection by replacing the aptamers modified on the sensing cathode surface.

自供电电化学传感器（SPES）作为一种新兴的传感方式，以其无需外接电源和检测便携等优点而备受关注。然而，开发具有增强输出信号和抗影响特性的spe仍然是一个挑战。为了解决这些问题，本文构建了一种新的基于锌空气电池（ZAB）的SPES，并以适配体作为特定的识别元素。在这种情况下，通过将Fe， Ni前驱体与先前制备的ZIF-8混合，合成了双金属Fe/Ni和N共掺杂碳纳米管和原位生长碳纳米管（Fe-Ni/N- c /CNT）。在中性条件下，Fe-Ni/N-C/CNT表现出0.831 V（相对于RHE）的高半波电位，与Fe-Ni/N-C/CNT组装的ZAB作为空气阴极产生了相当大的峰值功率密度（Pmax），为84.1 mW cm−2。选取凝血酶（TB）作为检测靶点，利用适体结构弯曲产生的界面位阻效应构建了“信号关闭”SPES，并以Pmax作为传感信号捕获靶点。所制备的zab基SPES的Pmax值与TB浓度的对数呈良好的线性关系，线性范围为2 × 10−4 nM ~ 2 × 10−2 nM，检出限为66.7 fM （LOD）。基于zab构建的SPES具有良好的抗干扰能力、稳定性和重复性，在实际应用中具有可行性和高效性。此外，通过替换传感阴极表面修饰的适体，该策略在其他目标检测中显示出很好的推广前景。

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引用次数: 0

Peptide-responsive photonic hydrogels integrated with deep learning assistance for early MMP-9 detection 肽响应光子水凝胶集成与深度学习辅助早期MMP-9检测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-24 DOI: 10.1016/j.bios.2026.118444

Yan Wang , Mingyi Liu , Xufang Liu , Dongxiao Hao , Yuzhu Wang , Yunfan Cai , Jing Li , Xiaoyu Miao , Yifan Zhang , Xiaojian Yang , Yongkang Bai , Chen Wang , Franklin R. Tay , Conrado Aparicio , Yao Zhao , Lina Niu

Matrix metalloproteinase-9 (MMP-9) is crucial for extracellular matrix remodeling, and its dysregulation is associated with inflammatory diseases and different forms of cancer. Conventional MMP-9 detection methods such as enzyme-linked immunosorbent assay (ELISA) are limited by complexity, expensive equipment, and lengthy antibody incubation times. These limitations prevent their use in point-of-care testing. An MMP-9 responsive photonic crystal (PC/PEG-M9SP) hydrogel has been developed to address these challenges. The hydrogel is synthesized from 4-arm polyethylene glycol-acrylate and an MMP-9 sensitive peptide via Michael-type addition reaction. Upon MMP-9-specific enzymatic cleavage, the hydrogel undergoes a structural reconfiguration, resulting in a distinct color shift. Integrated with a deep learning-based smartphone app, this platform enables both visual and quantitative detection within 10 min, achieving high sensitivity (10.60 nm mL/ng) and a detection limit of 0.62 ng/mL. Validation in complex biological fluids demonstrated strong concordance with ELISA, confirming the analytical reliability of the hydrogel. This system provides a rapid, portable, and cost-effective solution for accurate MMP-9 detection, with strong potential for clinical and point-of-care applications.

基质金属蛋白酶-9 （MMP-9）对细胞外基质重塑至关重要，其失调与炎症性疾病和不同形式的癌症有关。传统的MMP-9检测方法，如酶联免疫吸附试验（ELISA），受复杂性、昂贵的设备和较长的抗体孵育时间的限制。这些限制阻碍了它们在即时检测中的应用。一种MMP-9响应光子晶体（PC/PEG-M9SP）水凝胶已经开发出来，以解决这些挑战。该水凝胶由四臂聚乙二醇丙烯酸酯和MMP-9敏感肽通过michael型加成反应合成。在mmp -9特异性酶切后，水凝胶经历结构重构，导致明显的颜色偏移。该平台与基于深度学习的智能手机应用程序集成，可在10 min内实现视觉和定量检测，实现高灵敏度（10.60 nm mL/ng）和检测限0.62 ng/mL。在复杂生物流体中的验证与ELISA具有很强的一致性，证实了水凝胶分析的可靠性。该系统为准确检测MMP-9提供了快速、便携和经济高效的解决方案，在临床和护理点应用方面具有强大的潜力。

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