Biosensors and Bioelectronics最新文献_第3页

Cellular level cryo-neuromodulation using rapid and localized cooling device combined with microelectrode array

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-13 DOI: 10.1016/j.bios.2025.117257

Jaehyun Kim , Jong Seung Lee , Soyeon Noh , Eunseok Seo , Jungchul Lee , Taesung Kim , Seung-Woo Cho , Gunho Kim , Sung Soo Kim , Jungyul Park

Cryotherapy, a rapid and effective medical treatment utilizing low temperatures, has not been widely adopted in clinical practice due to a limited understanding of its mechanisms and efficacy. This challenge stems from the absence of methods for fast, precise, and localized spatiotemporal temperature control, as well as the lack of reliable real-time quantitative techniques for measuring and analyzing the effects of cooling. To address these limitations, this study introduces a cryo-neuromodulation platform that integrates a high-speed precision cooling device with a microelectrode array (MEA) system. This platform enables the investigation of cellular-level cryo-modulation of neuronal activity and its effects on surrounding cells, providing a novel framework for advancing research in cryotherapy and neuromodulation. Experiments show that neurons recovered fully within 1 min of cooling with a fast-cooling rate (−20 °C/s at cooling) and that silenced neurons can influence distant cells via a well-organized network. Extended cooling durations (e.g., 10 min) resulted in altered neuronal dynamics, including delayed recovery and reduced burst activity, highlighting the importance of precise control over cooling parameters. This device offers reversible neural control, with potential applications in both research and clinical settings, such as anesthesia, pain management and treatment of neurological disorders like neocortical seizures.

{"title":"Cellular level cryo-neuromodulation using rapid and localized cooling device combined with microelectrode array","authors":"Jaehyun Kim , Jong Seung Lee , Soyeon Noh , Eunseok Seo , Jungchul Lee , Taesung Kim , Seung-Woo Cho , Gunho Kim , Sung Soo Kim , Jungyul Park","doi":"10.1016/j.bios.2025.117257","DOIUrl":"10.1016/j.bios.2025.117257","url":null,"abstract":"<div><div>Cryotherapy, a rapid and effective medical treatment utilizing low temperatures, has not been widely adopted in clinical practice due to a limited understanding of its mechanisms and efficacy. This challenge stems from the absence of methods for fast, precise, and localized spatiotemporal temperature control, as well as the lack of reliable real-time quantitative techniques for measuring and analyzing the effects of cooling. To address these limitations, this study introduces a cryo-neuromodulation platform that integrates a high-speed precision cooling device with a microelectrode array (MEA) system. This platform enables the investigation of cellular-level cryo-modulation of neuronal activity and its effects on surrounding cells, providing a novel framework for advancing research in cryotherapy and neuromodulation. Experiments show that neurons recovered fully within 1 min of cooling with a fast-cooling rate (−20 °C/s at cooling) and that silenced neurons can influence distant cells via a well-organized network. Extended cooling durations (e.g., 10 min) resulted in altered neuronal dynamics, including delayed recovery and reduced burst activity, highlighting the importance of precise control over cooling parameters. This device offers reversible neural control, with potential applications in both research and clinical settings, such as anesthesia, pain management and treatment of neurological disorders like neocortical seizures.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117257"},"PeriodicalIF":10.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetically controlled all-in-one sensing platform for triple-mode detection of organophosphorus pesticides using DNA tetrahedrons-polydopamine reporters

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-12 DOI: 10.1016/j.bios.2025.117258

Haiwen Xiao, Jianing Ma, Zihan Liu, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang

Accurate and on-site detection of organophosphorus pesticides (OPs) in complex matrixes is important for environmental monitoring and food safety. Herein, a colorimetric (CL), photothermal (PT) and fluorescence (FL) triple-mode sensor was constructed for OPs detection based on magnetically controlled all-in-one platform, which was featured of specific recognition unit, abundant loading of DNA tetrahedrons-Fe³⁺-polydopamine (TDN-Fe³⁺-PDA) with peroxidase-like activity, and verifiable triple-mode signal output. Based on the aptamer-target recognition and efficient separation of MBs, large amount of TDN-Fe³⁺-PDA reporters was released. Taking full advantages of the supernatant and precipitation, OPs was sensitively detected with the TDN-Fe³⁺-PDA catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)-H₂O₂ system (CL mode), photothermal effect of TDN-Fe³⁺-PDA and oxidized TMB (oxTMB) (PT mode), and fluorescent signal of TDN-templated copper nanoclusters on MBs (FL mode). Using profenofos (Pro) as the model target, the triple-mode sensing platform realized sensitive detection of Pro with the detection limits 0.23 ng/mL, 0.40 ng/mL and 0.14 ng/mL for CL, PT and FL mode, respectively. The proposed strategy provided a simple and accurate method for on-site detection of OPs, holding promising application in environmental and food contamination monitoring.

{"title":"Magnetically controlled all-in-one sensing platform for triple-mode detection of organophosphorus pesticides using DNA tetrahedrons-polydopamine reporters","authors":"Haiwen Xiao, Jianing Ma, Zihan Liu, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang","doi":"10.1016/j.bios.2025.117258","DOIUrl":"10.1016/j.bios.2025.117258","url":null,"abstract":"<div><div>Accurate and on-site detection of organophosphorus pesticides (OPs) in complex matrixes is important for environmental monitoring and food safety. Herein, a colorimetric (CL), photothermal (PT) and fluorescence (FL) triple-mode sensor was constructed for OPs detection based on magnetically controlled all-in-one platform, which was featured of specific recognition unit, abundant loading of DNA tetrahedrons-Fe<sup>3+</sup>-polydopamine (TDN-Fe<sup>3+</sup>-PDA) with peroxidase-like activity, and verifiable triple-mode signal output. Based on the aptamer-target recognition and efficient separation of MBs, large amount of TDN-Fe<sup>3+</sup>-PDA reporters was released. Taking full advantages of the supernatant and precipitation, OPs was sensitively detected with the TDN-Fe<sup>3+</sup>-PDA catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)-H<sub>2</sub>O<sub>2</sub> system (CL mode), photothermal effect of TDN-Fe<sup>3+</sup>-PDA and oxidized TMB (oxTMB) (PT mode), and fluorescent signal of TDN-templated copper nanoclusters on MBs (FL mode). Using profenofos (Pro) as the model target, the triple-mode sensing platform realized sensitive detection of Pro with the detection limits 0.23 ng/mL, 0.40 ng/mL and 0.14 ng/mL for CL, PT and FL mode, respectively. The proposed strategy provided a simple and accurate method for on-site detection of OPs, holding promising application in environmental and food contamination monitoring.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117258"},"PeriodicalIF":10.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-based fluorescent probes for the diagnosis of tumor and image-guided surgery

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-12 DOI: 10.1016/j.bios.2025.117255

Ming-Ze Cai , Zhuan Wen , Hao-Ze Li , Yang Yang , Jian-Xiao Liang , Yu-Si Liao , Jing-Yao Wang , Li-Ying Wang , Ni-Yuan Zhang , Ken-ichiro Kamei , Hong-Wei An , Hao Wang

Fluorescent contrast agents are instrumental in amplifying signals, thereby enhancing the sensitivity and accuracy of live optical imaging. However, a significant proportion of traditional fluorescent contrast agents exhibit drawbacks such as short half-life, suboptimal biocompatibility, and inadequate tumor targeting, all of which impede effective imaging guidance. Peptides, derived from natural structures, offer a flexible modular design that can be precisely engineered and adjusted using synthetic methods to achieve specific biological activity and pharmacokinetic properties. They bind with designated receptors to exert their effects, demonstrating high specificity. The development of fluorescent probes based on peptides significantly overcomes the limitations of conventional contrast agents, offering superior performance. This article provides a comprehensive review of three strategies for constructing peptide-based fluorescent probes, delving into their distinct design concepts, mechanisms of action, and innovative aspects. It also highlights the potential applications of peptide-based fluorescent probes in tumor diagnosis and image-guided surgery, offering insights into their future clinical transformation.

{"title":"Peptide-based fluorescent probes for the diagnosis of tumor and image-guided surgery","authors":"Ming-Ze Cai , Zhuan Wen , Hao-Ze Li , Yang Yang , Jian-Xiao Liang , Yu-Si Liao , Jing-Yao Wang , Li-Ying Wang , Ni-Yuan Zhang , Ken-ichiro Kamei , Hong-Wei An , Hao Wang","doi":"10.1016/j.bios.2025.117255","DOIUrl":"10.1016/j.bios.2025.117255","url":null,"abstract":"<div><div>Fluorescent contrast agents are instrumental in amplifying signals, thereby enhancing the sensitivity and accuracy of live optical imaging. However, a significant proportion of traditional fluorescent contrast agents exhibit drawbacks such as short half-life, suboptimal biocompatibility, and inadequate tumor targeting, all of which impede effective imaging guidance. Peptides, derived from natural structures, offer a flexible modular design that can be precisely engineered and adjusted using synthetic methods to achieve specific biological activity and pharmacokinetic properties. They bind with designated receptors to exert their effects, demonstrating high specificity. The development of fluorescent probes based on peptides significantly overcomes the limitations of conventional contrast agents, offering superior performance. This article provides a comprehensive review of three strategies for constructing peptide-based fluorescent probes, delving into their distinct design concepts, mechanisms of action, and innovative aspects. It also highlights the potential applications of peptide-based fluorescent probes in tumor diagnosis and image-guided surgery, offering insights into their future clinical transformation.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117255"},"PeriodicalIF":10.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay for rapid and precise quantification of Hepatitis B Virus DNA

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-12 DOI: 10.1016/j.bios.2025.117256

Dongyang Cai , Yifan Wang , Ziyi Zhang , Enqi Huang , Na Yang , Xiao Yang , Ting Zhang , Hongting Wen , Yu Wang , Zhenhua Chen , Hongkai Wu , Dayu Liu

Recombinase polymerase amplification (RPA)-CRISPR/Cas12a assays have demonstrated remarkable potential for point-of-care detection of pathogens in resource-limited settings. Nevertheless, these assays fall short in delivering direct quantitative results due to the incompatibility between the RPA and CRISPR/Cas12a systems. To overcome this limitation, we developed a droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay in this study. By leveraging a microfluidic chip with a calabash-shaped microwell array, large-volume RPA droplets and small-volume CRISPR/Cas12a droplets were sequentially and size-selectively trapped, generating one-to-one droplet pairs. This spatial separation of the droplets eliminates the inhibitory effects of the CRISPR/Cas12a chemistry on RPA. Upon the completion of RPA, the CRISPR/Cas12a system can be activated by merging the paired droplets. This temporal separation of the RPA and CRISPR/Cas reactions allows for the accumulation of sufficient amplicons to efficiently unleash the collateral cleavage activity. The DIMERIC assay offers rapid quantification of nucleic acids, with the entire procedure being accomplished within 20 min. This assay was employed for the quantitative detection of Hepatitis B virus DNA from batched clinical serum samples, demonstrating a good correlation with qPCR (R² = 0.92033) and ddPCR (R² = 0.97337) outcomes. Consequently, the developed DIMERIC assay provides a valuable tool for rapid and precise quantification of pathogenic nucleic acids.

{"title":"Droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay for rapid and precise quantification of Hepatitis B Virus DNA","authors":"Dongyang Cai , Yifan Wang , Ziyi Zhang , Enqi Huang , Na Yang , Xiao Yang , Ting Zhang , Hongting Wen , Yu Wang , Zhenhua Chen , Hongkai Wu , Dayu Liu","doi":"10.1016/j.bios.2025.117256","DOIUrl":"10.1016/j.bios.2025.117256","url":null,"abstract":"<div><div>Recombinase polymerase amplification (RPA)-CRISPR/Cas12a assays have demonstrated remarkable potential for point-of-care detection of pathogens in resource-limited settings. Nevertheless, these assays fall short in delivering direct quantitative results due to the incompatibility between the RPA and CRISPR/Cas12a systems. To overcome this limitation, we developed a droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay in this study. By leveraging a microfluidic chip with a calabash-shaped microwell array, large-volume RPA droplets and small-volume CRISPR/Cas12a droplets were sequentially and size-selectively trapped, generating one-to-one droplet pairs. This spatial separation of the droplets eliminates the inhibitory effects of the CRISPR/Cas12a chemistry on RPA. Upon the completion of RPA, the CRISPR/Cas12a system can be activated by merging the paired droplets. This temporal separation of the RPA and CRISPR/Cas reactions allows for the accumulation of sufficient amplicons to efficiently unleash the collateral cleavage activity. The DIMERIC assay offers rapid quantification of nucleic acids, with the entire procedure being accomplished within 20 min. This assay was employed for the quantitative detection of Hepatitis B virus DNA from batched clinical serum samples, demonstrating a good correlation with qPCR (R<sup>2</sup> = 0.92033) and ddPCR (R<sup>2</sup> = 0.97337) outcomes. Consequently, the developed DIMERIC assay provides a valuable tool for rapid and precise quantification of pathogenic nucleic acids.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117256"},"PeriodicalIF":10.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular modulation of aggregation-induced luminescence for improving response sensing of DNA hydrogels

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-12 DOI: 10.1016/j.bios.2025.117254

Xuekun Bai, Chenxi Wang, Lei Huang, Hong Zhang, Jiangshan Zhang, Yunhua Cao, Li Wang, Wei Pang, Huanying Zhou, Zhixian Gao

Circadian rhythms are closely associated with human health, and the detection of relevant markers is essential to avoid circadian disorders. Here, we report a biosensing platform based on aggregation-induced emission for the sensitive detection of such markers. In this platform, the structure of 4,4′,4″,4‴-(ethene-1,1,2,2-tetrayl)tetrabenzaldehyde (ETBA) was modulated at the molecular level to 4′,4‴,4‴″,4⁗‴-(ethene-1,1,2,2-tetrayl)tetrakis([1,1′-biphenyl]-4-carbaldehyde) (ETBCA), thereby increasing the energy levels of the highest and lowest unoccupied molecular orbitals, reducing the energy gap, and enhancing the conjugation effect, ultimately improving the fluorescence properties of the molecule. ETBCA was the starting monomer used to synthesize ETBCA-loaded nanoparticles (ETBCANPs) with higher quantum yields and longer fluorescence lifetimes, which were then loaded onto responsive DNA hydrogels for the sensitive detection of melatonin. The resulting loaded hydrogel (ETBCANPs@Hydrogel) showed superior performance compared to hydrogels loaded with ETBA nanoparticles and quantum dots, with 2.9- and 3.6-fold higher sensitivities, respectively. The ETBCANPs@Hydrogel was able to detect melatonin in saliva and urine samples with limits of detection of 18.6 pg/mL and 10.5 pg/mL, respectively, recoveries of 94.2–107.5%, and satisfactory selectivity. In summary, the fluorescence performance of aggregation-induced emission molecules can be effectively improved by modulating their molecular structure, leading to the development of hydrogels for the sensitive sensing and detection of circadian rhythm disorders.

{"title":"Molecular modulation of aggregation-induced luminescence for improving response sensing of DNA hydrogels","authors":"Xuekun Bai, Chenxi Wang, Lei Huang, Hong Zhang, Jiangshan Zhang, Yunhua Cao, Li Wang, Wei Pang, Huanying Zhou, Zhixian Gao","doi":"10.1016/j.bios.2025.117254","DOIUrl":"10.1016/j.bios.2025.117254","url":null,"abstract":"<div><div>Circadian rhythms are closely associated with human health, and the detection of relevant markers is essential to avoid circadian disorders. Here, we report a biosensing platform based on aggregation-induced emission for the sensitive detection of such markers. In this platform, the structure of 4,4′,4″,4‴-(ethene-1,1,2,2-tetrayl)tetrabenzaldehyde (ETBA) was modulated at the molecular level to 4′,4‴,4‴″,4⁗‴-(ethene-1,1,2,2-tetrayl)tetrakis([1,1′-biphenyl]-4-carbaldehyde) (ETBCA), thereby increasing the energy levels of the highest and lowest unoccupied molecular orbitals, reducing the energy gap, and enhancing the conjugation effect, ultimately improving the fluorescence properties of the molecule. ETBCA was the starting monomer used to synthesize ETBCA-loaded nanoparticles (ETBCANPs) with higher quantum yields and longer fluorescence lifetimes, which were then loaded onto responsive DNA hydrogels for the sensitive detection of melatonin. The resulting loaded hydrogel (ETBCANPs@Hydrogel) showed superior performance compared to hydrogels loaded with ETBA nanoparticles and quantum dots, with 2.9- and 3.6-fold higher sensitivities, respectively. The ETBCANPs@Hydrogel was able to detect melatonin in saliva and urine samples with limits of detection of 18.6 pg/mL and 10.5 pg/mL, respectively, recoveries of 94.2–107.5%, and satisfactory selectivity. In summary, the fluorescence performance of aggregation-induced emission molecules can be effectively improved by modulating their molecular structure, leading to the development of hydrogels for the sensitive sensing and detection of circadian rhythm disorders.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117254"},"PeriodicalIF":10.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and selective detection of TP53 mutations in cancer using a novel conductometric biosensor

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-11 DOI: 10.1016/j.bios.2025.117252

Ganganath S. Perera , Xiaomin Huang , Fateme Akhlaghi Bagherjeri , Chinmayee Manesh Joglekar , Paul Leo , Pascal Duijf , Madhu Bhaskaran , Sharath Sriram , Chamindie Punyadeera

Tumour protein p53 (TP53) is a tumour suppressor gene that is frequently mutated in cancers. Traditional TP53 detection methods, such as polymerase chain reactions, are time-consuming and demand skilled laboratory personnel. As an alternative, in the current study, we have demonstrated a high resistivity silicon (HR-Si) based conductometric biosensor designed for the rapid and specific identification of TP53 point mutations directly at the point-of-need. This biosensor accurately detected R248Q and R248W point mutant single strand DNA (ssDNA) as models, in real-time. Both R248Q and R248W mutant ssDNA exhibited a limit of detection (LOD) of 0.5 ng/mL in human plasma. The selectivity studies revealed that both R248Q and R248W mutant ssDNA can be detected 10 × lower molar content against their wild-type ssDNA. Validation of the sensor using clinical samples harbouring known TP53 mutations demonstrated a sensitivity of 100%, a specificity of 100%, and a LOD of 2.5 ng/mL. This precision biosensing platform at the point-of-need has the potential to revolutionise cancer diagnostics.

{"title":"Rapid and selective detection of TP53 mutations in cancer using a novel conductometric biosensor","authors":"Ganganath S. Perera , Xiaomin Huang , Fateme Akhlaghi Bagherjeri , Chinmayee Manesh Joglekar , Paul Leo , Pascal Duijf , Madhu Bhaskaran , Sharath Sriram , Chamindie Punyadeera","doi":"10.1016/j.bios.2025.117252","DOIUrl":"10.1016/j.bios.2025.117252","url":null,"abstract":"<div><div><em>Tumour protein p53</em> (<em>TP53</em>) is a tumour suppressor gene that is frequently mutated in cancers. Traditional <em>TP53</em> detection methods, such as polymerase chain reactions, are time-consuming and demand skilled laboratory personnel. As an alternative, in the current study, we have demonstrated a high resistivity silicon (HR-Si) based conductometric biosensor designed for the rapid and specific identification of <em>TP53</em> point mutations directly at the point-of-need. This biosensor accurately detected R248Q and R248W point mutant single strand DNA (ssDNA) as models, in real-time. Both R248Q and R248W mutant ssDNA exhibited a limit of detection (LOD) of 0.5 ng/mL in human plasma. The selectivity studies revealed that both R248Q and R248W mutant ssDNA can be detected 10 × lower molar content against their wild-type ssDNA. Validation of the sensor using clinical samples harbouring known <em>TP53</em> mutations demonstrated a sensitivity of 100%, a specificity of 100%, and a LOD of 2.5 ng/mL. This precision biosensing platform at the point-of-need has the potential to revolutionise cancer diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117252"},"PeriodicalIF":10.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly integrated and compact transduction strategy for multidimensional sensing: Near infrared light mediated transmission of optical, electrical and visual signals for ovarian cancer marker assay.

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-08 DOI: 10.1016/j.bios.2025.117224

Shupei Zhang, Yitian Huang, Lihong Gao, Yanjie Chen, Hong Dai

Mediating effective signal conversion strategies through intermediate parameters to develop easily integrated smart sensing platforms remains a challenge. Herein, a compact and miniaturized optical, electrical and visual sensor was constructed through reasonably designing and assembling three sub-sensors, which can simultaneously and independently feedback signal changes brought by near-infrared (NIR) light. Concretely, the thermal effect induced by multifunctional V₂C MQDs@polyaniline@NiFe₂O₄ with excellent photothermal performance was employed to regulate the signal output of luminescence, color and resistance in multilayer sensing chip, which the chip was formed by attaching thermochromic paper and thermoelectric module layer-by-layer to the nonconductive backside of transparent indium tin oxide electrode modified with electrochemiluminescent (ECL) signal probe. Additionally, the pleasant luminescent property of V₂C MQDs and outstanding electrocatalytic ability of NiFe₂O₄ endowed this probe with another role in providing highly sensitive ECL signal transduction. As a result, the multifunctional probe could convert light into thermal energy under the NIR light irradiation, realizing temperature enhanced ECL response, temperature initiated chameleon paper (CoCl₂·6H₂O) with thermochromic behavior for intuitive temperature visualization output and thermoelectrical module for high-resolution resistance analysis. Ultimately, the photomodulated multimode sensing platform realized sensitive lipolysis stimulated lipoprotein receptor detection with a wide linear range from 10^-6 to 10 ng/mL, excellent selectivity, good stability and high accuracy. The successful application of intermediate parameter driving and crosstalk-free multidimensional signal processing, offering an innovative regulating strategy for promoting the development of multifunctional signal conversion devices.

{"title":"Highly integrated and compact transduction strategy for multidimensional sensing: Near infrared light mediated transmission of optical, electrical and visual signals for ovarian cancer marker assay.","authors":"Shupei Zhang, Yitian Huang, Lihong Gao, Yanjie Chen, Hong Dai","doi":"10.1016/j.bios.2025.117224","DOIUrl":"https://doi.org/10.1016/j.bios.2025.117224","url":null,"abstract":"<p><p>Mediating effective signal conversion strategies through intermediate parameters to develop easily integrated smart sensing platforms remains a challenge. Herein, a compact and miniaturized optical, electrical and visual sensor was constructed through reasonably designing and assembling three sub-sensors, which can simultaneously and independently feedback signal changes brought by near-infrared (NIR) light. Concretely, the thermal effect induced by multifunctional V<sub>2</sub>C MQDs@polyaniline@NiFe<sub>2</sub>O<sub>4</sub> with excellent photothermal performance was employed to regulate the signal output of luminescence, color and resistance in multilayer sensing chip, which the chip was formed by attaching thermochromic paper and thermoelectric module layer-by-layer to the nonconductive backside of transparent indium tin oxide electrode modified with electrochemiluminescent (ECL) signal probe. Additionally, the pleasant luminescent property of V<sub>2</sub>C MQDs and outstanding electrocatalytic ability of NiFe<sub>2</sub>O<sub>4</sub> endowed this probe with another role in providing highly sensitive ECL signal transduction. As a result, the multifunctional probe could convert light into thermal energy under the NIR light irradiation, realizing temperature enhanced ECL response, temperature initiated chameleon paper (CoCl<sub>2</sub>·6H<sub>2</sub>O) with thermochromic behavior for intuitive temperature visualization output and thermoelectrical module for high-resolution resistance analysis. Ultimately, the photomodulated multimode sensing platform realized sensitive lipolysis stimulated lipoprotein receptor detection with a wide linear range from 10<sup>-6</sup> to 10 ng/mL, excellent selectivity, good stability and high accuracy. The successful application of intermediate parameter driving and crosstalk-free multidimensional signal processing, offering an innovative regulating strategy for promoting the development of multifunctional signal conversion devices.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117224"},"PeriodicalIF":10.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-08 DOI: 10.1016/j.bios.2025.117233

Jing Xu , Xinqi Luo , Hanxiao Chen , Bin Guo , Lijun Jia , Fu Wang

Sensitive microRNA (miRNA) detection is crucial for cancer diagnosis. Self-powered biosensors that are used for miRNA detection show the advantages of no external power supply, mild reaction conditions, portability, etc., but still face the challenges of low efficiency of solid electrode adsorption enzyme and insufficient enzyme active site. Here, the MnS@MoS₂ composite electrode substrate boosts enzyme load, accelerates electron transfer, and enhances detection. Catalytic hairpin self-assembly (CHA) and Hybridization chain reaction (HCR) cascade bio-signal amplification enables current signal amplification via molecular recognition. When miRNA-199a is present, CHA and HCR trigger signal cascade amplification, achieving high sensitivity and specificity while powering the system. The sensor has a 0.5 fmol/L - 100 pmol/L linear response and a 0.14 fmol/L limit of detection (LOD). Additionally, the test signal is transmitted to a smartphone interface via Bluetooth, enabling portable, enabling portable, real-time detection. Our work shows this self-powered biosensor offers a new path for ultrasensitive miRNA detection, aids rapid disease biomarker monitoring, and broadens self-powered sensor use in medical diagnosis.

{"title":"A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers","authors":"Jing Xu , Xinqi Luo , Hanxiao Chen , Bin Guo , Lijun Jia , Fu Wang","doi":"10.1016/j.bios.2025.117233","DOIUrl":"10.1016/j.bios.2025.117233","url":null,"abstract":"<div><div>Sensitive microRNA (miRNA) detection is crucial for cancer diagnosis. Self-powered biosensors that are used for miRNA detection show the advantages of no external power supply, mild reaction conditions, portability, etc., but still face the challenges of low efficiency of solid electrode adsorption enzyme and insufficient enzyme active site. Here, the MnS@MoS₂ composite electrode substrate boosts enzyme load, accelerates electron transfer, and enhances detection. Catalytic hairpin self-assembly (CHA) and Hybridization chain reaction (HCR) cascade bio-signal amplification enables current signal amplification via molecular recognition. When miRNA-199a is present, CHA and HCR trigger signal cascade amplification, achieving high sensitivity and specificity while powering the system. The sensor has a 0.5 fmol/L - 100 pmol/L linear response and a 0.14 fmol/L limit of detection (LOD). Additionally, the test signal is transmitted to a smartphone interface via Bluetooth, enabling portable, enabling portable, real-time detection. Our work shows this self-powered biosensor offers a new path for ultrasensitive miRNA detection, aids rapid disease biomarker monitoring, and broadens self-powered sensor use in medical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117233"},"PeriodicalIF":10.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A HClO-activated BODIPY based ratiometric fluorescent probe with dual near-infrared channels for differentiating cancerous cells from normal cells and surgical guidance of tumor resection

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-07 DOI: 10.1016/j.bios.2025.117247

Yueyin Liang , Tongtong Xu , Song Xu , Xu Xu , Liping Wang , Zhonglong Wang , Shifa Wang

Cancer (malignant tumor), a serious disease with a high mortality rate, early-stage diagnosis and treatment are very important for cancer therapy. The average concentration of reactive oxygen species (ROS) in cancer cells is ten times higher than in normal cells, and the overexpression of hypochlorous acid (HClO) is closely associated with cancer progression. Herein, a novel near-infrared (NIR) Golgi apparatus-targetable (GA) fluorescent probe GA-BOD-S was synthesized using the BODIPY and phenothiazine as the basic fluorescence framework for HClO monitoring. GA-BOD-S exhibited a colorimetric and ratiometric dual-mode recognition for HClO with the excellent merits including low detection limit (35 nM), fast response time (within 13 s), and exceptional GA targeting capability (Pearson's coefficient 0.98). Notably, both fluorescence emission wavelengths of GA-BOD-S before and after reaction with HClO are located in the NIR region, significantly enhancing the quality of fluorescence imaging in vivo. Cellular experiments revealed that GA-BOD-S can effectively visualize both endogenous and exogenous HClO within cells and living zebrafish. GA-BOD-S was successfully employed for high-contrast imaging of cancer cells and normal cells based on the differences in intracellular ROS levels, and achieved in-situ imaging of tumors in vivo. Furthermore, the precision of tumor resection surgery was significantly improved under the guidance of fluorescence probe GA-BOD-S, which provided a new perspective for the diagnosis and treatment of cancer disease.

{"title":"A HClO-activated BODIPY based ratiometric fluorescent probe with dual near-infrared channels for differentiating cancerous cells from normal cells and surgical guidance of tumor resection","authors":"Yueyin Liang , Tongtong Xu , Song Xu , Xu Xu , Liping Wang , Zhonglong Wang , Shifa Wang","doi":"10.1016/j.bios.2025.117247","DOIUrl":"10.1016/j.bios.2025.117247","url":null,"abstract":"<div><div>Cancer (malignant tumor), a serious disease with a high mortality rate, early-stage diagnosis and treatment are very important for cancer therapy. The average concentration of reactive oxygen species (ROS) in cancer cells is ten times higher than in normal cells, and the overexpression of hypochlorous acid (HClO) is closely associated with cancer progression. Herein, a novel near-infrared (NIR) Golgi apparatus-targetable (GA) fluorescent probe <strong>GA-BOD-S</strong> was synthesized using the BODIPY and phenothiazine as the basic fluorescence framework for HClO monitoring. <strong>GA-BOD-S</strong> exhibited a colorimetric and ratiometric dual-mode recognition for HClO with the excellent merits including low detection limit (35 nM), fast response time (within 13 s), and exceptional GA targeting capability (Pearson's coefficient 0.98). Notably, both fluorescence emission wavelengths of <strong>GA-BOD-S</strong> before and after reaction with HClO are located in the NIR region, significantly enhancing the quality of fluorescence imaging in vivo. Cellular experiments revealed that <strong>GA-BOD-S</strong> can effectively visualize both endogenous and exogenous HClO within cells and living zebrafish. <strong>GA-BOD-S</strong> was successfully employed for high-contrast imaging of cancer cells and normal cells based on the differences in intracellular ROS levels, and achieved in-situ imaging of tumors in vivo. Furthermore, the precision of tumor resection surgery was significantly improved under the guidance of fluorescence probe <strong>GA-BOD-S</strong>, which provided a new perspective for the diagnosis and treatment of cancer disease.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117247"},"PeriodicalIF":10.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-channel fluorescent probe with mitochondria-immobilization: Detecting polarity and viscosity during mitophagy

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-07 DOI: 10.1016/j.bios.2025.117246

Yue Huang , Yang Liu , Chuan Dong , Qi Zan , Feng Feng , Ruibing Wang , Shaomin Shuang

Mitophagy is a key pathway for regulating mitochondrial quality and quantity which is essential for the preservation of cellular homeostasis. Mitophagy process may be accompanied by changes of the mitochondrial microenvironments. The multifunctional fluorescent probe is crucial for the precise detection of multiple microenvironments, which is vital for the visualization of mitophagy. Herein, a mitochondria-immobilized fluorescent probe DPP was designed and fabricated to visualize mitophagy by monitoring polarity and viscosity in dual-channel. The DPP is characterized by “D-π-A″ structure, which provides the basis for the intramolecular charge transfer (ICT) and twisted intramolecular charge transfer (TICT) platform, enabling dual-channel responses to polarity and viscosity at emission wavelengths of 487 nm and 656 nm, respectively. The significant wavelength gap (169 nm) between the above channels prevents signal crosstalk. Additionally, the incorporation of 1, 4-dibenzyl chloride grants the probe mitochondrial immobilization capabilities, avoiding the leak of probe due to mitochondrial depolarization during autophagy. The DPP accumulates in mitochondria and monitors polarity and viscosity changes in green and red channels, respectively. Notably, the investigation of the relationship between polarity and viscosity revealed that an increase in viscosity is accompanied by a decrease in polarity. The mitophagy was effectively observed through the induction of DPP by rapamycin, with a particular emphasis on the increase in viscosity and decrease in polarity. Thus, DPP offers a powerful tool for a deeper understanding of the physiological and pathological processes associated with mitophagy and are regulated by various microenvironmental parameters.

{"title":"A dual-channel fluorescent probe with mitochondria-immobilization: Detecting polarity and viscosity during mitophagy","authors":"Yue Huang , Yang Liu , Chuan Dong , Qi Zan , Feng Feng , Ruibing Wang , Shaomin Shuang","doi":"10.1016/j.bios.2025.117246","DOIUrl":"10.1016/j.bios.2025.117246","url":null,"abstract":"<div><div>Mitophagy is a key pathway for regulating mitochondrial quality and quantity which is essential for the preservation of cellular homeostasis. Mitophagy process may be accompanied by changes of the mitochondrial microenvironments. The multifunctional fluorescent probe is crucial for the precise detection of multiple microenvironments, which is vital for the visualization of mitophagy. Herein, a mitochondria-immobilized fluorescent probe <strong>DPP</strong> was designed and fabricated to visualize mitophagy by monitoring polarity and viscosity in dual-channel. The <strong>DPP</strong> is characterized by “D-π-A″ structure, which provides the basis for the intramolecular charge transfer (ICT) and twisted intramolecular charge transfer (TICT) platform, enabling dual-channel responses to polarity and viscosity at emission wavelengths of 487 nm and 656 nm, respectively. The significant wavelength gap (169 nm) between the above channels prevents signal crosstalk. Additionally, the incorporation of 1, 4-dibenzyl chloride grants the probe mitochondrial immobilization capabilities, avoiding the leak of probe due to mitochondrial depolarization during autophagy. The <strong>DPP</strong> accumulates in mitochondria and monitors polarity and viscosity changes in green and red channels, respectively. Notably, the investigation of the relationship between polarity and viscosity revealed that an increase in viscosity is accompanied by a decrease in polarity. The mitophagy was effectively observed through the induction of <strong>DPP</strong> by rapamycin, with a particular emphasis on the increase in viscosity and decrease in polarity. Thus, <strong>DPP</strong> offers a powerful tool for a deeper understanding of the physiological and pathological processes associated with mitophagy and are regulated by various microenvironmental parameters.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117246"},"PeriodicalIF":10.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0