Biosensors and Bioelectronics最新文献_第3页

An AIE-based ratiometric fluorescent probe for highly selective detection of H2S in plant stress responses 基于 AIE 的比率荧光探针，用于高选择性检测植物胁迫反应中的 H2S

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-19 DOI: 10.1016/j.bios.2024.116798

Hydrogen sulfide (H₂S) has emerged as a crucial signaling molecule in plant stress responses, playing a significant role in regulating various physiological and biochemical processes. In this study, we report an aggregation-induced emission (AIE)-based ratiometric fluorescent probe TPN-H₂S for the highly selective detection of H₂S in plant tissues. The probe exhibited excellent sensitivity and selectivity towards H₂S over other analytes, enabling real-time monitoring of H₂S dynamics in living cell. Furthermore, the AIE-based ratiometric probe TPN-H₂S allowed for accurate quantification of H₂S levels, providing valuable insights into the spatiotemporal distribution of Cys metabolism produces H₂S. Importantly, the physiological pathways and signaling mechanisms of H₂S production of was investigated in plant tissues under Cr and nano-plastics stress. Utilizing a high-throughput screening approach, we identified exogenous substances such as calcium chloride (CaCl₂) and abscisic acid (ABA) that could induce higher level of H₂S production during the stress response in plants. Overall, those findings demonstrate the potential of the AIE-based ratiometric fluorescent probe TPN-H₂S as a powerful tool for unraveling the role of H₂S in plant stress responses and pave the way for further exploration of H₂S-mediated signaling pathways in plants.

硫化氢（H2S）已成为植物胁迫响应中的重要信号分子，在调节各种生理和生化过程中发挥着重要作用。本研究报告了一种基于聚集诱导发射（AIE）的比率荧光探针 TPN-H2S，用于高选择性地检测植物组织中的 H2S。与其他分析物相比，该探针对 H2S 具有极高的灵敏度和选择性，能够实时监测活细胞中 H2S 的动态。此外，基于 AIE 的比率测量探针 TPN-H2S 还能准确量化 H2S 水平，为深入了解 Cys 代谢产生 H2S 的时空分布提供了宝贵的信息。重要的是，研究了铬和纳米塑料胁迫下植物组织产生 H2S 的生理途径和信号机制。利用高通量筛选方法，我们确定了氯化钙（CaCl2）和脱落酸（ABA）等外源物质，它们可以在植物胁迫响应期间诱导更高水平的 H2S 生成。总之，这些发现证明了基于 AIE 的比率荧光探针 TPN-H2S 的潜力，它是揭示 H2S 在植物胁迫响应中的作用的有力工具，并为进一步探索 H2S 介导的植物信号通路铺平了道路。

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引用次数: 0

Rapid and direct detection of m6A methylation by DNAzyme-based and smartphone-assisted electrochemical biosensor 利用基于 DNA 酶和智能手机辅助的电化学生物传感器快速直接检测 m6A 甲基化

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-19 DOI: 10.1016/j.bios.2024.116788

m⁶A methylation detection is crucial for understanding RNA functions, revealing disease mechanisms, guiding drug development and advancing epigenetics research. Nevertheless, high-throughput sequencing and liquid chromatography-based traditional methods still face challenges to rapid and direct detection of m⁶A methylation. Here we report a DNAzyme-based and smartphone-assisted electrochemical biosensor for rapid detection of m⁶A. We initially identified m⁶A methylation-sensitive DNAzyme mutants through site mutation screening. These mutants were then combined with tetrahedral DNA to modify the electrodes, creating a 3D sensing interface. The detection of m⁶A was accomplished by using DNAzyme to capture and cleave the m⁶A sequence. The electrochemical biosensor detected the m⁶A sequence at nanomolar concentrations with a low detection limit of 0.69 nM and a wide detection range from 10 to 10⁴ nM within 60 min. As a proof of concept, the 3′-UTR sequence of rice was selected as the m⁶A analyte. Combined with a smartphone, our biosensor shows good specificity, sensitivity, and easy-to-perform features, which indicates great prospects in the field of RNA modification detection and epigenetic analysis.

m6A 甲基化检测对于了解 RNA 功能、揭示疾病机制、指导药物开发和推进表观遗传学研究至关重要。然而，高通量测序和基于液相色谱的传统方法在快速直接检测 m6A 甲基化方面仍面临挑战。在此，我们报告了一种基于 DNA 酶和智能手机辅助的快速检测 m6A 的电化学生物传感器。我们最初通过位点突变筛选确定了对 m6A 甲基化敏感的 DNA 酶突变体。然后将这些突变体与四面体 DNA 相结合，对电极进行修饰，形成了一个三维传感界面。利用 DNA 酶捕获并裂解 m6A 序列，实现了对 m6A 的检测。该电化学生物传感器能在纳摩尔浓度下检测到 m6A 序列，检测限低至 0.69 nM，60 分钟内的检测范围从 10 nM 到 104 nM。作为概念验证，我们选择了水稻的 3'-UTR 序列作为 m6A 分析物。结合智能手机，我们的生物传感器显示出良好的特异性、灵敏度和易操作性，这预示着它在 RNA 修饰检测和表观遗传分析领域的巨大前景。

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引用次数: 0

Flexible AIE/PCM composite fiber with biosensing of alcohol, fluorescent anti-counterfeiting and body thermal management functions 具有酒精生物传感、荧光防伪和人体热管理功能的柔性 AIE/PCM 复合纤维

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-19 DOI: 10.1016/j.bios.2024.116799

Alcohol sensing plays a critical role in medical detection and personal health management. AIE materials with high sensitivity, selectivity and fast response have been widely used in biosensing, but their application in the field of alcohol sensing still needs further research and development. Furthermore, developing flexible phase change materials (PCMs) is significant for the research of human-body thermal management. In this study, a kind of flexible polyacrylonitrile (PAN)/polyvinylpyrrolidone (PVP)/polyethylene glycol (PEG)/Py-CH (pyrene-based AIE molecule)/SiO₂@h-BN composite fiber textile (PAB) with alcohol sensing performance, writable fluorescence property, and human body thermal management function has been prepared via electrospinning technique. The PAN/PVP fiber matrix successfully integrated AIE fluorescent sensing material and PCM into a multi-functional composite with great shape stability. Owing to the introduction of novel pyrene-based Py-CH with AIE characteristic, this innovative textile exhibited wonderful fluorescent properties, including sensitive alcohol fluorescence sensing, writable fluorescence performance and variable temperature fluorescence. Furthermore, proposed PAB textile delivered a high energy storage density of 87∼90 J/g, excellent thermal reliability, great comprehensive mechanical flexibility and enhanced thermal conductivity for flexible human body thermal management. Hence, this flexible multifunctional AIE/PCM composite sensing textiles can be widely used in alcohol sensing, fluorescence anti-counterfeiting and flexible body thermal management.

酒精传感在医学检测和个人健康管理中发挥着至关重要的作用。具有高灵敏度、高选择性和快速响应的 AIE 材料已广泛应用于生物传感领域，但其在酒精传感领域的应用仍需进一步研究和开发。此外，开发柔性相变材料（PCM）对人体热管理研究也具有重要意义。本研究通过电纺技术制备了一种具有酒精传感性能、可写荧光特性和人体热管理功能的柔性聚丙烯腈（PAN）/聚乙烯吡咯烷酮（PVP）/聚乙二醇（PEG）/Py-CH（芘基 AIE 分子）/SiO2@h-BN 复合纤维织物（PAB）。PAN/PVP 纤维基体成功地将 AIE 荧光传感材料和 PCM 融合成了一种形状稳定的多功能复合材料。由于引入了具有 AIE 特性的新型芘基 Py-CH，这种创新纺织品表现出了优异的荧光特性，包括灵敏的酒精荧光传感、可写荧光性能和变温荧光。此外，所提出的 PAB 纺织品还具有 87∼90 J/g 的高储能密度、出色的热可靠性、极佳的综合机械柔韧性和更强的导热性，可用于柔性人体热管理。因此，这种柔性多功能 AIE/PCM 复合传感纺织品可广泛应用于酒精传感、荧光防伪和柔性人体热管理。

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引用次数: 0

Intracellularly manipulable aggregation of the aggregation-induced emission luminogens 细胞内可操控的聚合诱导发光发光剂

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-19 DOI: 10.1016/j.bios.2024.116800

Biophotonics has seen significant advancements with the development of optical imaging techniques facilitating the noninvasive detection of biologically relevant species. Aggregation-induced emission (AIE) materials have emerged as a novel class of luminogens exhibiting enhanced luminescence or photodynamic efficiency in the aggregated state, making them ideal for biomedical applications. The intracellularly controlled aggregation of aggregate-induced emission luminogens (AIEgens) enables high-resolution imaging of intracellular targets and diagnosis of related diseases, and enables disease therapy by exploiting the novel properties of aggregates. This review provides an in-depth analysis of the strategies employed to modulate the aggregation of AIEgens, focusing on the importance of molecular modifications to improve hydrophilicity and achieve precise control over the intercellular aggregation of AIEgens. Furthermore, the representative applications of AIEgens in bioimaging, such as enzyme activity monitoring, protein tracking, organelle function monitoring, and in vivo tumor-specific therapeutics, are reviewed. Additionally, we outline the challenges and future opportunities for AIE research, emphasizing the importance of the strategies for realizing the precisely controllable aggregation of AIEgens inside cells and the need for extending AIEgens’ absorption and emission wavelengths. This review aims to elucidate the rational development of responsive AIEgens for advanced biomedical applications.

随着光学成像技术的发展，生物光子学取得了重大进展，促进了对生物相关物种的无创检测。聚合诱导发光（AIE）材料是一类新型发光剂，在聚合状态下发光或光动力效率增强，是生物医学应用的理想材料。聚合诱导发射光源（AIEgens）的细胞内可控聚合可实现细胞内靶标的高分辨率成像和相关疾病的诊断，利用聚合体的新特性还可进行疾病治疗。本综述深入分析了调节 AIEgens 聚集的策略，重点关注分子修饰对提高亲水性和精确控制 AIEgens 细胞间聚集的重要性。此外，我们还综述了 AIEgens 在生物成像中的代表性应用，如酶活性监测、蛋白质追踪、细胞器功能监测和体内肿瘤特异性治疗。此外，我们还概述了 AIE 研究面临的挑战和未来的机遇，强调了实现 AIEgens 在细胞内精确可控聚集的策略的重要性，以及扩展 AIEgens 吸收和发射波长的必要性。本综述旨在阐明如何为先进的生物医学应用合理开发多模态 AIEgens。

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引用次数: 0

Application of smart-responsive hydrogels in nucleic acid and nucleic acid-based target sensing: A review 智能响应水凝胶在核酸和基于核酸的目标传感中的应用：综述

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-19 DOI: 10.1016/j.bios.2024.116803

In recent years, nucleic acid-related sensing and detection have become essential in clinical diagnostics, treatment and genotyping, especially in connection with the Human Genome Project and the COVID-19 pandemic. Many traditional nucleic acid-related sensing strategies have been employed in analytical chemistry, including fluorescence, colorimetric and chemiluminescence methods. However, their key limitation is the lack of understanding of the interaction during analysis, particularly at the 3D matrix level close to biological tissue. To address this issue, smart-responsive hydrogels are increasingly used in biosensing due to their hydrophilic and biocompatible properties. By combining smart-responsive hydrogels with traditional nucleic acid-related sensing, biological microenvironments can be mimicked, and targets can be easily accessed and diffused, making them ideal for nucleic acid sensing. This review focuses on utilizing smart-responsive hydrogels for nucleic acid-related sensing and detection, including nucleic acid detection, other nucleic acid-based analyte detection and nucleic acid-related sensing platforms applying nucleic acid as sensing tools in hydrogels. Additionally, the analytical mechanisms of smart-responsive hydrogels with the combination of various detection platforms such as optical and electrochemical techniques are described. The limitations of using smart-responsive hydrogels in nucleic acid-related sensing and proposed possible solutions are also discussed. Lastly, the future challenge of smart-responsive hydrogels in nucleic acid-related sensing is explored. Smart-responsive hydrogels can be used as biomimetic materials to simulate the extracellular matrix, achieve biosensing, and exhibit great potential in nucleic acid-related sensing. They serve as a valuable complement to traditional detection and analytical methods.

近年来，与核酸有关的传感和检测已成为临床诊断、治疗和基因分型的关键，特别是在人类基因组计划和 COVID-19 大流行病方面。分析化学中采用了许多传统的核酸相关传感策略，包括荧光、比色和化学发光方法。然而，这些方法的主要局限是缺乏对分析过程中相互作用的了解，尤其是在接近生物组织的三维基质层面。为了解决这个问题，智能响应水凝胶因其亲水性和生物相容性，越来越多地被用于生物传感。通过将智能响应水凝胶与传统的核酸相关传感技术相结合，可以模拟生物微环境，使目标物易于接触和扩散，从而使其成为核酸传感的理想选择。本综述重点介绍利用智能响应水凝胶进行核酸相关传感和检测，包括核酸检测、其他基于核酸的分析物检测以及在水凝胶中应用核酸作为传感工具的核酸相关传感平台。此外，还介绍了智能响应水凝胶与光学和电化学技术等各种检测平台相结合的分析机制。还讨论了在核酸相关传感中使用智能响应水凝胶的局限性，并提出了可能的解决方案。最后，探讨了智能响应水凝胶在核酸相关传感中的未来挑战。智能响应水凝胶可用作模拟细胞外基质的仿生材料，实现生物传感，在核酸相关传感方面具有巨大潜力。它们是传统检测和分析方法的重要补充。

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引用次数: 0

Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody 可定制的 OpenGUS 免疫测定：使用β-葡萄糖醛酸酶开关和无标记抗体的均质检测系统

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-18 DOI: 10.1016/j.bios.2024.116796

We developed a customizable OpenGUS immunoassay that enables rapid and sensitive detection of analytes without requiring antibody modification. This immunoassay employs label-free whole antibodies, an antibody-binding Z domain (ZD) derived from Staphylococcal protein A, and a β-glucuronidase (GUS) switch mutant, allowing for easy replacement of antibodies to tailor the immunoassays for various targeted antigens. The working principle is that the OpenGUS probe, the fusion protein of ZD and a GUS switch, converts the antibody-antigen interaction into GUS activation in a one-pot reaction. To enhance the signal-to-background ratio of the immunoassay, a GUS switch mutant that exhibits reduced background activation was developed by screening several additional mutations at the diagonal interface residue H514. Moreover, we optimized the composition of the reaction buffer, including organic solvents, salt, and surfactant. Under optimal conditions, we customized OpenGUS immunoassays for Cry j 1, human C-reactive protein, and human lactoferrin, achieving around 10–20-fold maximum fluorescence (15 min) or colorimetric (2 h) responses with picomolar to low nanomolar level detection limit, simply by using commercially available IgGs. Additionally, the three analytes were successfully detected in complex matrices similar to those used in practical applications. We believe that this customizable OpenGUS immunoassay will pave the way for the prompt development of rapid and sensitive homogeneous immunoassays for point-of-care diagnostics, high-throughput testing, and onsite environmental assessments.

我们开发了一种可定制的 OpenGUS 免疫测定，无需对抗体进行修饰即可快速灵敏地检测分析物。这种免疫测定采用了无标记全抗体、源自葡萄球菌蛋白 A 的抗体结合 Z 结构域 (ZD) 和 β-葡糖醛酸酶 (GUS) 开关突变体，从而可以轻松替换抗体，针对各种目标抗原定制免疫测定。其工作原理是，OpenGUS 探针是 ZD 和 GUS 开关的融合蛋白，通过一锅反应将抗体与抗原的相互作用转化为 GUS 激活。为了提高免疫测定的信噪比，我们通过筛选对角界面残基 H514 的几个额外突变，开发出了一种能降低背景激活的 GUS 开关突变体。此外，我们还优化了反应缓冲液的成分，包括有机溶剂、盐和表面活性剂。在最佳条件下，我们定制了针对 Cry j 1、人 C 反应蛋白和人乳铁蛋白的 OpenGUS 免疫测定，只需使用市售的 IgG，就能实现约 10-20 倍的最大荧光反应（15 分钟）或比色反应（2 小时），检测限为皮摩尔至低纳摩尔水平。此外，我们还成功地在与实际应用类似的复杂基质中检测到了这三种分析物。我们相信，这种可定制的 OpenGUS 免疫测定将为快速开发用于护理点诊断、高通量测试和现场环境评估的快速、灵敏的均相免疫测定铺平道路。

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引用次数: 0

A novel triple-signal biosensor based on ZrFe-MOF@PtNPs for ultrasensitive aflatoxins detection 基于 ZrFe-MOF@PtNPs 的新型三重信号生物传感器，用于超灵敏黄曲霉毒素检测

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-18 DOI: 10.1016/j.bios.2024.116797

The development of more sensitive, stable, and portable biosensors is crucial for meeting the growing demands of diverse and complex detection environments. MOF-based nanozymes have emerged as excellent optical reporters, making them ideal signal donors for constructing multi-signal lateral flow immunoassays (LFIA). In this study, a ZrFe-MOF@PtNPs nanocomposite was synthesized by uniformly depositing platinum nanoparticles (PtNPs) onto the surface of ZrFe-MOFs using an impregnation-reduction method. The ZrFe-MOF@PtNPs exhibited broad absorption spectra, excellent peroxidase-like activity (SA = 21.77 U/mg), high solvent stability, and efficient antibody binding capability. A portable LFIA platform was developed based on ZrFe-MOF@PtNPs and a smartphone for the targeted detection of carcinogenic aflatoxins. This method enabled the readout of colorimetric, fluorescent, and catalytic signals, significantly enhancing detection sensitivity, ensuring result accuracy, and expanding the dynamic detection range. For aflatoxin M1, the calculation of the detection limit of the three signal modes reached as low as 0.0062 ng/mL, which is two orders of magnitude more sensitive than AuNPs-LFIA (0.1839 ng/mL). This study provides effective guidance for multifunctional modification of MOFs and serves as a reference for the application of MOF-based nanozymes in point-of-care biosensors.

开发更加灵敏、稳定和便携的生物传感器对于满足日益增长的多样化和复杂检测环境的需求至关重要。基于 MOF 的纳米酶已成为出色的光学报告物，使其成为构建多信号侧向流动免疫分析（LFIA）的理想信号供体。本研究采用浸渍还原法在 ZrFe-MOFs 表面均匀沉积铂纳米颗粒（PtNPs），合成了 ZrFe-MOF@PtNPs 纳米复合材料。ZrFe-MOF@PtNPs 具有宽吸收光谱、优异的过氧化物酶样活性（SA=21.77 U/mg）、高溶剂稳定性和高效的抗体结合能力。基于 ZrFe-MOF@PtNPs 和智能手机开发了一种便携式 LFIA 平台，用于靶向检测致癌黄曲霉毒素。该方法可读出比色、荧光和催化信号，大大提高了检测灵敏度，确保了结果的准确性，并扩大了动态检测范围。对于黄曲霉毒素 M1，三种信号模式的检测限计算结果低至 0.0062 ng/mL，比 AuNPs-LFIA 的灵敏度（0.1839 ng/mL）高出两个数量级。该研究为MOFs的多功能修饰提供了有效指导，为基于MOFs的纳米酶在床旁生物传感器中的应用提供了参考。

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引用次数: 0

Multi-functional, conformal systems with ultrathin crystalline-silicon-based bioelectronics for characterization of intraocular pressure and ocular surface temperature 基于超薄晶体硅的多功能保形生物电子系统，用于鉴定眼内压和眼表温度

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-18 DOI: 10.1016/j.bios.2024.116786

Technologies that established in vivo evaluations of soft-tissue biomechanics and temperature are essential to biological research and clinical diagnostics, particularly for a wide range of eye-related diseases such as glaucoma. Of importance are advanced bioelectronic devices for high-precise monitoring of intraocular pressure (IOP) and various ocular temperatures, as clinically proven uses for glaucoma diagnosis. Existing characterization methods are temporary, single point, and lack microscale resolution, failing to measure continuous IOP fluctuation across the long-term period. Here, this work presents a multi-functional smart contact lens, capable of rapidly capturing IOP fluctuation and ocular surface temperature (OST) for assistance for clinical use. The microscale device design is programmable and determined by finite element analysis simulation, with detailed experiments of ex vivo porcine eyeballs. Such compact bioelectronics can provide high-precise measurement with sensitivity of 0.03% mmHg⁻¹ and 1.2 Ω °C⁻¹ in the range of Δ2∼50 mmHg and 30–50 °C, respectively. In vivo tests of bio-integration with a living rabbit can evaluate real-time IOP fluctuation and OST, as of biocompatibility assessments verified through cellular and animal experiments. The resultant bioelectronic devices for continuous precise characterization of living eyeballs can offer broad utility for hospital diagnosis of a wide range of eye-related disorders.

对软组织生物力学和温度进行活体评估的技术对生物研究和临床诊断至关重要，尤其是对青光眼等多种眼部相关疾病。其中最重要的是用于高精度监测眼压（IOP）和各种眼部温度的先进生物电子设备，这些设备已在青光眼诊断中得到临床验证。现有的表征方法都是临时的、单点的，缺乏微观分辨率，无法测量长期持续的眼压波动。本研究提出了一种多功能智能隐形眼镜，能够快速捕捉眼压波动和眼表温度（OST），为临床使用提供帮助。这种微型装置的设计是可编程的，由有限元分析模拟确定，并在活体猪眼球上进行了详细实验。这种紧凑型生物电子器件可提供高精度测量，在 Δ2∼50 mmHg 和 30∼50 °C 范围内灵敏度分别为 0.03% mmHg-1 和 1.2 Ω ℃-1。在活体兔子身上进行的生物集成测试可评估实时眼压波动和 OST，以及通过细胞和动物实验验证的生物兼容性评估。由此产生的生物电子装置可对活体眼球进行连续精确的表征，为医院诊断各种眼部相关疾病提供广泛的用途。

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引用次数: 0

Simplified single neuron model for robust local pulse wave velocity sensing using a tetherless bioimpedance device 利用无对偶生物阻抗装置进行鲁棒性局部脉搏波速度传感的简化单神经元模型

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-18 DOI: 10.1016/j.bios.2024.116793

Pulse arrival time (PAT), Pulse transit time (PTT), and Pulse Wave Velocity (PWV) have all been used as metrics for assessing a number of cardiovascular applications, including arterial stiffness and cuffless blood pressure monitoring. These have been measured using various sensing methods, including electrocardiogram (ECG) with photoplethysmogram (PPG), two PPG sensors, or two Bioimpedance (BioZ) sensors. Our study addresses the mathematical inaccuracies of previous bioimpedance approaches and incorporates PTT weights for the peak-peak (PTTpp), middle-middle (PTTmm), and foot-foot (PTTff) segments of the sensing signal into a single neuron model to determine a more accurate and stable PWV. In addition, we developed a tetherless bioimpedance device and compared our PTT estimation approaches, which yielded PWV across six subjects and two different arteries. Specifically, using our model, we found that the most reliable combination of weights corresponding to PTTpp, PTTmm, and PTTff was (0.260, 0.704, 0.036) for the brachial artery and (0.104, 0.858, 0.038) for radial artery. This model consistently yielded stable values across repetitions, with PWV values of 5.2 m/s, 5.3 m/s, and 5.9 m/s for the brachial artery and values of 5.8 m/s, 6.6 m/s, and 6.5 m/s for the radial artery. This system and model offer the possibility of obtaining higher reliability PTT and PWV values yielding better monitoring of cardiovascular health measures such as blood pressure and arterial stiffness.

脉搏到达时间 (PAT)、脉搏传输时间 (PTT) 和脉搏波速度 (PWV) 都被用作评估心血管应用的指标，包括动脉僵化和无袖带血压监测。这些指标是通过各种传感方法测量的，包括心电图（ECG）与光敏血流图（PPG）、两个 PPG 传感器或两个生物阻抗（BioZ）传感器。我们的研究解决了以往生物阻抗方法在数学上的不准确性，并将传感信号的峰-峰（PTTpp）、中-中（PTTmm）和足-足（PTTff）段的 PTT 权重纳入单神经元模型，以确定更准确、更稳定的脉搏波速度。此外，我们还开发了一种无拴生物阻抗装置，并比较了我们的 PTT 估算方法，得出了六个受试者和两条不同动脉的脉搏波速度。具体来说，使用我们的模型，我们发现对应于 PTTpp、PTTmm 和 PTTff 的最可靠权重组合对于肱动脉是（0.260, 0.704, 0.036），对于桡动脉是（0.104, 0.858, 0.038）。该模型在各次重复中都能获得稳定的数值，肱动脉的脉搏波速度值分别为 5.2m/s、5.3m/s 和 5.9m/s，桡动脉的脉搏波速度值分别为 5.8m/s、6.6m/s 和 6.5m/s。该系统和模型可以获得可靠性更高的 PTT 和脉搏波速度值，从而更好地监测心血管健康状况，如血压和动脉僵化。

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引用次数: 0

Ultrasensitive detection of holoTC for analysis of Vitamin B12 levels using Ag2MoO4 deposited PEDOT sensing platform 利用 Ag2MoO4 沉积 PEDOT 传感平台超灵敏检测全四氯化碳以分析维生素 B12 水平

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2024-09-18 DOI: 10.1016/j.bios.2024.116783

Vitamin B12 is an essential micronutrient required for the proper functioning of the human body. Vitamin B12 deficiency is primarily causative of various neurolological disorders alongwith recurrence of oral ulcers and burning sensations which are early signs of condition such as pernicious anemia. Other complications associated with Vitamin B12 deficiency include risk of heart failure due to anemia, risk of developing autoimmune disorders and gastric cancer. Therefore, to obstruct these communal health issues, early detection of Vit B12 is highly needed. However, screening of vitamin B12 insufficiency is hindered by the low sensitivity of the conventional vitamin B12 test. Holotranscobalamin (holoTC) is an early indicator of the negative vitamin B12 balance as it is the first protein to decline in the serum. We report a novel impedimetric immunosensor based on flower-like poly (3,4-ethylenedioxythiophene) (PEDOT) nanostructural film impregnated with silver molybdate nanoparticles (Ag₂MoO₄ NPs) deposited on fluorine-doped tin oxide electrode. The prepared electrodes were characterized by Field emission scanning electron microscopy (FE-SEM) with energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and electrochemical studies. The activated anti-holoTC antibody was immobilized and optimized to capture the target in a response time of 15 min. The electrochemical performance of the sensor was carried out by using the electrochemical impedance spectroscopy technique (EIS) and a good linear relationship between ΔR_ct and holoTC was obtained in the range from 0.1 pg mL⁻¹ to 100 ng mL⁻¹ with a detection limit of 0.093 pg mL⁻¹. The proposed sensor was successfully applied in human serum samples for holoTC detection. The experimental results showed that the immunosensor is highly selective towards holoTC and presented an acceptable stability of 20 days with reproducibility RSD ≤4%. To the best of our knowledge, this is the first developed electrochemical immunosensor for holoTC detection.

维生素 B12 是人体正常运作所必需的微量营养素。缺乏维生素 B12 主要会导致各种神经系统疾病，以及口腔溃疡复发和烧灼感，这是恶性贫血等疾病的早期症状。与维生素 B12 缺乏有关的其他并发症包括因贫血导致心力衰竭的风险、患自身免疫性疾病和胃癌的风险。因此，要阻止这些常见的健康问题，就必须及早发现。然而，目前总维生素 B12 检测的灵敏度较低，阻碍了维生素 B12 缺乏症的筛查。全曲钴胺（holoTC）是维生素 B12 负平衡的早期指标，因为它是血清中第一个下降的蛋白质。我们报告了一种新型阻抗免疫传感器，该传感器基于沉积在掺氟氧化锡电极上的浸渍有钼酸银纳米颗粒（Ag2MoO₄ NPs）的花状聚（3,4-亚乙二氧基噻吩）（PEDOT）纳米结构薄膜。利用场发射扫描电子显微镜（FE-SEM）、能量色散 X 射线光谱（EDS）、X 射线衍射（XRD）和电化学研究对制备的电极进行了表征。经过固定和优化的活化抗 HoloTC 抗体可在 15 分钟的响应时间内捕获目标物。利用电化学阻抗光谱技术（EIS）对传感器的电化学性能进行了测定，结果表明，在 0.1 pg mL-1 至 100 ng mL-1 的范围内，ΔRct 与 holoTC 之间存在良好的线性关系，检测限为 0.093 pg mL-1。所提出的传感器被成功应用于人血清样品中的 holoTC 检测。实验结果表明，该免疫传感器对 holoTC 具有高度选择性，其稳定性在 20 天内均可接受，再现性 RSD ≤4%。据我们所知，这是首个用于检测 holoTC 的电化学免疫传感器。

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