Biosensors and Bioelectronics最新文献_第3页

Multicolor SERS-encoded immuno-cocktail for longitudinal precise tracking of CTCs phenotypes in lung cancer therapeutics 多色sers编码免疫鸡尾酒用于肺癌治疗中ctc表型的纵向精确跟踪。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-12-01 DOI: 10.1016/j.bios.2025.118277

Min Fan , Zongyang Yu , Kan Luo , Ping Chen , Duo Lin , Yating Lin , Xueliang Lin , Jingbo Chen , Shangyuan Feng

Tumor heterogeneity and drug resistance remain major obstacles to the implementation of personalized therapeutic strategies in lung cancer. Circulating tumor cell (CTC)-based liquid biopsy provides a powerful, minimally invasive tool for tracking dynamic changes in tumor heterogeneity and elucidating mechanisms of treatment response and drug resistance. Herein, we present a portable, field-deployable surface-enhanced Raman scattering (SERS) immunoprobe platform capable of multiplexed detection of CTC membrane biomarkers to monitor tumor heterogeneity throughout the course of therapy. Utilizing a cocktail of multicolor-encoded immunoprobes, the platform achieves single-cell phenotypic resolution via antigen-specific spectral fingerprinting, validated by flow cytometry. It demonstrates ultra-sensitive CTC quantification (limit of detection of 1.7 cells/mL) and precise molecular subtyping across four cell lines (HCC827, A549, BEAS-2B, and HeLa). Integrated with orthogonal partial least squares discriminant analysis (OPLS-DA), the platform accurately distinguishes clinically relevant cell types with 99 % classification accuracy, successfully stratifying metastatic versus non-metastatic lung cancer (n = 24) and capturing treatment-driven CTC evolution in longitudinal studies (n = 7). Our results highlight a transformative strategy for real-time interception of drug resistance trajectories, positioning dynamic CTC phenotyping as a critical tool for adaptive therapy and prognostic assessment in precision oncology.

肿瘤异质性和耐药性仍然是肺癌个体化治疗策略实施的主要障碍。基于循环肿瘤细胞（CTC）的液体活检为追踪肿瘤异质性的动态变化和阐明治疗反应和耐药机制提供了一种强大的微创工具。在此，我们提出了一种便携式，现场可部署的表面增强拉曼散射（SERS）免疫探针平台，能够多重检测CTC膜生物标志物，以监测整个治疗过程中的肿瘤异质性。利用多色编码免疫探针的混合物，该平台通过抗原特异性光谱指纹识别实现单细胞表型分辨率，并通过流式细胞术验证。它具有超灵敏的CTC定量（检测限为1.7个细胞/mL）和精确的分子分型，适用于4个细胞系（HCC827、A549、BEAS-2B和HeLa）。结合正交偏最小二乘判别分析（OPLS-DA），该平台以99%的分类准确率准确区分临床相关细胞类型，成功地对转移性和非转移性肺癌进行分层（n = 24），并在纵向研究中捕获治疗驱动的CTC进化（n = 7）。我们的研究结果强调了实时拦截耐药轨迹的变革性策略，将动态CTC表型定位为精确肿瘤学中适应性治疗和预后评估的关键工具。

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引用次数: 0

An ultrasensitive miRNA-25 aptasensor based on Co@CNTs nanoaggregates for early detection of pancreatic cancer 基于Co@CNTs纳米聚集体的超灵敏miRNA-25适体传感器用于胰腺癌的早期检测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-12-01 DOI: 10.1016/j.bios.2025.118288

Yunzhi Han , Min Zhou , Mengjiao Zhu , Wei Zhang , Yu Liu , Tao Liu , Zhenyu Chu , Wanqin Jin

miRNA-25 is a critical and specific biomarker for the early diagnosis of pancreatic cancer. However, its sensitive and efficient detection remains challenging due to its extremely low abundance and time-consuming procedures of conventional methods. In this work, we proposed an ultrasensitive electrochemical aptasensor for rapid determination of miRNA-25 within 30 min using cubic Co@CNTs nanoaggregates (CC-NAs) as the signal nano-amplifier. This unique nanoarchitecture was in situ constructed through precisely controlled pyrolysis of CoCo Prussian blue analogues as a single precursor. The resulting CC-NAs exhibit excellent conductivity, electrocatalytic activity, and abundant binding sites for immobilizing capture DNA, thereby enhancing the recognition of reverse-transcribed DNA (tDNA) corresponding to miRNA-25. The aptasensor achieved an ultralow detection limit of 0.3 fM (3 × 10⁻¹⁶ M) and an ultrawide linear range from 1 fM to 10 nM, along with high reliability and accuracy in real sample analysis. This CC-NAs based aptasensor holds great promise for facilitating rapid and ultrasensitive early screening and warning of various cancers, providing a powerful technique for aiding clinical diagnosis across diverse scenarios.

miRNA-25是胰腺癌早期诊断的重要特异性生物标志物。然而，由于其极低的丰度和传统方法的耗时过程，其敏感和高效的检测仍然具有挑战性。在这项工作中，我们提出了一种超灵敏的电化学容体传感器，用于在30分钟内快速测定miRNA-25，使用立方Co@CNTs纳米聚集体（CC-NAs）作为信号纳米放大器。这种独特的纳米结构是通过精确控制可可普鲁士蓝类似物作为单一前体的热解原位构建的。所得到的CC-NAs具有优异的导电性、电催化活性和丰富的固定捕获DNA的结合位点，从而增强了对miRNA-25对应的逆转录DNA （tDNA）的识别。该传感器具有0.3 fM （3 × 10−16 M）的超低检出限和1 fM至10 nM的超宽线性范围，在实际样品分析中具有很高的可靠性和准确性。这种基于CC-NAs的适体传感器在促进各种癌症的快速和超灵敏的早期筛查和预警方面具有很大的前景，为帮助临床诊断提供了一种强大的技术。

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引用次数: 0

Wash-free electrochemical biosensor for CpG methylation using a quasi-direct electron transfer type enzyme as the labeling enzyme 以准直接电子转移型酶为标记酶的CpG甲基化无水洗电化学生物传感器。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-12-01 DOI: 10.1016/j.bios.2025.118285

Mizuki Tomizawa , Runa Hosaka , Daimei Miura , Kazunori Ikebukuro

We developed an electrochemical detection system for CpG methylation using an enzyme with the quasi–direct electron transfer (quasi–DET) ability for labeling. We directly fused the methyl–CpG binding domain (MBD), which binds to the oligonucleotide bearing methylated CpG, and glucose dehydrogenase (GDH), which was used for labeling, to construct MBD–GDH. MBD–GDH was directly modified with amine–reactive phenazine ethosulfate (arPES) as a mediator to add its quasi–direct electron transfer (quasi–DET) ability. When MBD bound to the methylated CpG oligonucleotide, which was immobilized on the electrode, MBD–GDH was brought into close proximity to the electrode. Upon adding glucose to the electrode, electrons from GDH and arPES were transferred to the electrode surface. The arPES–modified MBD–GDH showed the quasi–DET ability when the target DNA was immobilized on the electrode and it showed a linear response to the increase in methylation level in the range of 0–100 %. We succeeded in developing a wash–free electrochemical detection system using labeling enzymes with the quasi–DET ability.

我们利用一种具有准直接电子转移（准det）标记能力的酶开发了一种CpG甲基化的电化学检测系统。我们直接将与携带甲基化CpG寡核苷酸结合的甲基-CpG结合域（MBD）与用于标记的葡萄糖脱氢酶（GDH）融合构建了MBD-GDH。以氨基反应性苯那嗪乙硫酸酯（arPES）为介质直接修饰MBD-GDH，增加其准直接电子转移（准det）能力。当MBD与固定在电极上的甲基化CpG寡核苷酸结合时，MBD- gdh被带到靠近电极的地方。在电极上加入葡萄糖后，来自GDH和arPES的电子被转移到电极表面。当目标DNA固定在电极上时，arpes修饰的MBD-GDH表现出准det能力，甲基化水平的增加在0- 100%范围内呈线性响应。我们成功地开发了一种使用具有准det能力的标记酶的免洗涤电化学检测系统。

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引用次数: 0

Smart bioelectronic facemask for non-invasive detection of respiratory infections 用于无创检测呼吸道感染的智能生物电子口罩。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-29 DOI: 10.1016/j.bios.2025.118269

Mi Shi , Lirong Wang , Ru Feng , Qiang Li , Ruiping Zhang , Tailin Xu

With the escalating global burden of respiratory infections and their recurrent outbreaks, the development of simple, accurate, and non-invasive point-of-care diagnostic technologies has become an urgent public health priority. In this study, we present a smart bioelectronic facemask designed for noninvasive detection of respiratory pathogens, which facilitates collection of exhaled aerosols and enables rapid, highly sensitive, and accurate detection of SARS-CoV-2. This platform incorporates flexible microfluidic chip and a miniaturized circuit boards into conventional face masks without compromising user comfort. The microfluidic chip integrates a porous polycarbonate (PC) membrane for continuous aerosol sampling, and a recombinase polymerase amplification (RPA)-coupled electrochemical biosensor for specific viral detection. This wearable platform seamlessly integrates the entire workflow of exhaled aerosol collection, lysis, nucleic acid amplification, and real-time detection, which enables the completion of analysis within 30 min and achieves a detection limit as low as 0.19 copy/μL. Validation using simulated exhaled breath samples demonstrated 96.7 % accuracy, 95.0 % sensitivity, and 100 % specificity compared to RT-qPCR. By combining non-invasive sampling and wearable molecular diagnostics, this smart bioelectronic facemask constitutes a viable platform for point-of-care pathogen detection, offering a timely and practical solution for use in high-risk and resource-limited settings.

随着全球呼吸道感染负担的不断增加及其反复爆发，开发简单、准确和非侵入性的即时诊断技术已成为一项紧迫的公共卫生重点。在本研究中，我们设计了一种用于呼吸道病原体无创检测的智能生物电子口罩，该口罩有助于收集呼出的气溶胶，并实现对SARS-CoV-2的快速、高灵敏度和准确检测。该平台将柔性微流控芯片和小型化电路板集成到传统的口罩中，而不会影响用户的舒适度。该微流控芯片集成了用于连续气溶胶采样的多孔聚碳酸酯（PC）膜和用于特定病毒检测的重组酶聚合酶扩增（RPA）耦合电化学生物传感器。该可穿戴平台无缝集成了呼出气溶胶采集、裂解、核酸扩增、实时检测的整个工作流程，可在30分钟内完成分析，检测限低至0.19 copy/μL。与RT-qPCR相比，使用模拟呼气样本验证的准确性为96.7%，灵敏度为95.0%，特异性为100%。通过结合非侵入性采样和可穿戴分子诊断，这种智能生物电子口罩构成了一个可行的即时护理病原体检测平台，为高风险和资源有限的环境提供了及时和实用的解决方案。

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引用次数: 0

Engineering direct electron transfer in single-atom-bridged nanozymes for enhanced oxidase-like activity at neutral pH. 工程直接电子转移在单原子桥接纳米酶增强氧化酶样活性在中性pH。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-29 DOI: 10.1016/j.bios.2025.118262

Chaolei Hua, Mingyang Jiang, Guan Liu, Shifei Sun, Yiqiao Hu, Tianyu Song, Chenchen Chu, Yubei Zhang, Yijie Chen, Xue-Feng Yu, Wenhua Zhou, Shengyong Geng

The constrained catalytic activity of conventional nanozymes under neutral pH conditions presents a fundamental challenge that limits their practical applicability in physiological environments. To overcome this limitation, we design a MnO₂-supported gold single-atom nanozyme (MnO₂-Au SAN) through a freeze-assisted photochemical reduction approach. A key feature of this system is the formation of a strong electronic metal-support interaction (EMSI), which enables efficient electron transfer between atomically dispersed Au sites, the MnO₂ substrate, and adsorbed molecular species. By integrating spectroscopic characterization with kinetic measurements, we show that this electron-transfer mechanism significantly enhances oxidase-like activity at neutral pH. The MnO₂-Au SAN achieves an exceptionally low Michaelis constant of 0.040 mM for TMB oxidation, superior to that of most reported nanozymes. The catalytic mechanism, investigated through radical scavenging and electron spin resonance analyses, is associated with the enhanced generation of holes and hydroxyl radicals under neutral conditions. When applied to a lateral flow immunoassay for the detection of influenza A virus, the MnO₂-Au SAN enables a detection limit of 20 pg/mL using TMB-based signal amplification. This work presents a general EMSI-driven strategy for enhancing nanozyme activity under physiological pH conditions, underscoring the critical importance of atomic-level electron transfer in emulating natural enzymatic reactions.

传统纳米酶在中性pH条件下的催化活性受到限制，这对其在生理环境中的实际应用提出了根本性的挑战。为了克服这一限制，我们通过冷冻辅助光化学还原方法设计了mno2负载的金单原子纳米酶（MnO2-Au SAN）。该系统的一个关键特征是形成了一个强大的电子金属支撑相互作用（EMSI），它可以在原子分散的Au位点、MnO2底物和吸附的分子物种之间实现有效的电子转移。通过将光谱表征与动力学测量相结合，我们发现这种电子转移机制显著提高了中性ph下类似氧化酶的活性。MnO2-Au SAN在氧化TMB时达到了极低的米切里斯常数0.040 mM，优于大多数报道的纳米酶。通过自由基清除和电子自旋共振分析，研究了催化机制与中性条件下空穴和羟基自由基的生成增强有关。当应用于检测甲型流感病毒的侧流免疫分析时，MnO2-Au SAN使用基于tmb的信号放大，可实现20 pg/mL的检测限。这项工作提出了一种在生理pH条件下增强纳米酶活性的通用emsi驱动策略，强调了原子水平电子转移在模拟自然酶促反应中的关键重要性。

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引用次数: 0

Endogenous peroxynitrite-responsive bioorthogonal SERS nanoprobe for ratiometric Raman imaging assessment of drug-induced liver injury 内源性过氧亚硝酸盐反应性生物正交SERS纳米探针用于药物性肝损伤的比例拉曼成像评估

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-29 DOI: 10.1016/j.bios.2025.118276

Yafei Ma , Jingjie Suo , Qian Wang , Zhan Wang , Shusheng Zhang , Sheng Chen

Peroxynitrite (ONOO⁻) plays a critical role in drug-induced liver injury (DILI), making it essential to elucidate its relationship with disease progression to improve early diagnosis and treatment. Nevertheless, the accurate and reliable detection of ONOO⁻ in biological systems with surface-enhanced Raman scattering (SERS) nanoprobes remains challenging. In this study, inspired by the specific reaction between α-ketoamide and ONOO⁻, we designed the first endogenous ONOO⁻-responsive bioorthogonal SERS nanoprobe to investigate drug-induced liver injury through ratiometric SERS imaging in the Raman-silent region. The SERS nanoprobe is composed of an alkyne-tagged internal standard (4-TEB) and a cyano-based ONOO⁻ responsive unit (DPCO), which shows a remarkable ratiometric SERS change (I₂₂₃₃/I₂₁₅₃) in the Raman-silent region for ONOO⁻ with high selectivity and sensitivity, and detection limits as low as 21.5 nM. We successfully applied the probe to track ONOO⁻ dynamics at single-cell resolution during its induction and inhibition in living cells. Subsequently, it was used to monitor APAP-induced ONOO⁻ fluctuations in HepG2 cells and to map ONOO⁻ distribution in the livers of DILI mice, thereby revealing insights into drug-induced hepatotoxicity and its remediation. It is anticipated that the nanoprobe holds significant potential for elucidating DILI mechanisms and advancing clinical diagnostics.

过氧亚硝酸盐（ONOO−）在药物性肝损伤（DILI）中起着至关重要的作用，阐明其与疾病进展的关系对于改善早期诊断和治疗至关重要。然而，利用表面增强拉曼散射（SERS）纳米探针准确可靠地检测生物系统中的ONOO−仍然具有挑战性。在这项研究中，受α-酮酰胺与ONOO−特异性反应的启发，我们设计了第一个内源性ONOO−响应的生物正交SERS纳米探针，通过拉曼沉默区比例SERS成像来研究药物性肝损伤。SERS纳米探针由炔标记的内标（4-TEB）和氰基ONOO -响应单元（DPCO）组成，在ONOO -拉曼沉默区表现出显著的比例SERS变化（I2233/I2153），具有很高的选择性和灵敏度，检测限低至21.5 nM。我们成功地应用探针在单细胞分辨率下跟踪ONOO−在活细胞中的诱导和抑制过程。随后，它被用于监测apap诱导的HepG2细胞中ONOO−的波动，并绘制DILI小鼠肝脏中ONOO−的分布，从而揭示药物诱导的肝毒性及其修复的见解。预计纳米探针在阐明DILI机制和推进临床诊断方面具有重大潜力。

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引用次数: 0

Large-scale manufacturing engineered 3D cardiac spheroids for dynamic electrophysiological biosensing and precise drug assessment 用于动态电生理生物传感和精确药物评估的大规模制造工程3D心脏球体。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-29 DOI: 10.1016/j.bios.2025.118267

Qianwen Xiong , Jiajin Xue , Dongxin Xu , Jiaru Fang , Xuelian Lyu , Qunchen Yuan , Weiwei Chen , Zhigang Gao , Ning Hu , Chunlian Qin

Cardiovascular disease remains a leading cause of mortality worldwide. Three-dimensional cardiac models hold great promise to study disease mechanisms and develop new therapies. In this work, we establish a 3D cardiac spheroids biosensing platform that integrates large-scale 3D cardiac spheroids preparation and dynamic electrophysiological recording. Cardiac spheroids are cultured for consecutive days to optimize their robust self-assembly and uniform morphology. The optimal cardiac spheroids exhibit rhythmic electrical and mechanical signals, indicating functional maturation and biomimetic replication of cardiac electromechanical properties. Moreover, the cardiac spheroids models could precisely assess the norepinephrine administration, revealing the positive regulatory mechanisms of β1-AR signaling and GRK/β-arrestin-mediated negative feedback in 3D cardiac spheroids. RNA sequencing further highlights the advantages of 3D cardiac spheroids over 2D monolayer cultures and validates the underlying mechanisms of drugs. The developed cardiac spheroids biosensing platform serves as a powerful and scalable tool for cardiac tissue modeling, functional assessment, and pharmacological evaluation.

心血管疾病仍然是全世界死亡的主要原因。三维心脏模型在研究疾病机制和开发新的治疗方法方面具有很大的前景。在这项工作中，我们建立了一个集大规模三维心脏球体制备和动态电生理记录为一体的三维心脏球体生物传感平台。心脏球体连续培养数日，以优化其强大的自组装和均匀的形态。最佳心脏球体表现出节律性的电信号和机械信号，表明心脏机电特性的功能成熟和仿生复制。此外，心脏球体模型可以准确评估去甲肾上腺素给药，揭示了β1-AR信号和GRK/β-arrestin介导的三维心脏球体负反馈的正调控机制。RNA测序进一步强调了3D心脏球体相对于2D单层培养物的优势，并验证了药物的潜在机制。开发的心脏球体生物传感平台可作为心脏组织建模，功能评估和药理评估的强大且可扩展的工具。

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引用次数: 0

Corrigendum to "A bacteriophage protein-driven platform for rapid and precise diagnosis of bacterial pathogens from blood samples" [Biosens. Bioelectron. 289 (2025) 117896]. “噬菌体蛋白驱动的快速准确诊断血液样本中细菌病原体的平台”的勘误表[Biosens]。生物电子学学报，2009(5):387 - 398。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-29 DOI: 10.1016/j.bios.2025.118266

Jinhuan Liu, Yijue Wang, Xin Fan, Yuanfeng Li, Yong Liu, Hongping Wan, Xinghong Zhao

引用次数: 0

A novel MOFs-functionalized microelectrode-based biosensor for real-time monitoring of hydrogen peroxide from tumor cells 一种新型mofs功能化微电极生物传感器，用于实时监测肿瘤细胞中的过氧化氢

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-28 DOI: 10.1016/j.bios.2025.118268

Zhiquan Luo , Zizhen Ming , Wanlei Gao , Panhui Yang , Beibei Lu , Ji Gao , Zhiyong Guo , Fang Bao , Hui Cong , Qinghui Jin

Sensitive real-time monitoring of hydrogen peroxide (H₂O₂) in the tumor microenvironment is crucial for comprehending its dual biological effects and enhancing therapeutic strategies. Existing techniques for real-time detection of H₂O₂ in tumor cells often suffer from limitations such as low sensitivity, poor selectivity, and difficult achievement of non-invasive dynamic monitoring. In this study, a high-performance electrochemical biosensor was developed based on metal-organic frameworks (MOFs) for non-invasive, real-time detection of H₂O₂ released by tumor cells. The sensor, featuring an integrated thin-film electrode constructed via microelectromechanical system (MEMS) technology, combined a biosensing interface film of AuNPs/ZIF-8@ZIF-67 and XC-72 carbon black with a poly(dimethylsiloxane) cell culture chamber, enabling real-time monitoring of H₂O₂ during cell culture. The sensor exhibited a wide linear detection range (1–350 μM), a low detection limit (0.908 μM), high stability (RSD = 4.57 %), and strong anti-interference (under 15 %). Compared to a system with discrete large-area electrodes, this integrated platform not only retained the detection performance but also effectively overcame the limitations associated with in situ cell culture.The system was successfully applied to monitor oxidative stress in various tumor cells under ascorbic acid stimulation. It effectively distinguished differences in H₂O₂ levels between tumor cells and normal cells, thereby underscoring its potential in tumor research and drug screening.This study provides an efficient and reliable platform for investigating tumor-related oxidative stress and assessing therapeutic responses.

对肿瘤微环境中过氧化氢（H2O2）的敏感实时监测对于了解其双重生物学效应和提高治疗策略至关重要。现有的肿瘤细胞中H2O2实时检测技术往往存在灵敏度低、选择性差、难以实现无创动态监测等局限性。本研究开发了一种基于金属有机框架（MOFs）的高性能电化学生物传感器，用于无创、实时检测肿瘤细胞释放的H2O2。该传感器采用微机电系统（MEMS）技术构建的集成薄膜电极，将AuNPs/ZIF-8@ZIF-67和XC-72炭黑的生物传感界面膜与聚二甲基硅氧烷细胞培养室结合在一起，能够实时监测细胞培养过程中的H2O2。该传感器线性检测范围宽（1 ~ 350 μM），检出限低（0.908 μM），稳定性高（RSD = 4.57%），抗干扰能力强（小于15%）。与具有离散大面积电极的系统相比，该集成平台不仅保留了检测性能，而且有效地克服了原位细胞培养相关的局限性。该系统已成功应用于抗坏血酸刺激下多种肿瘤细胞的氧化应激监测。它可以有效区分肿瘤细胞与正常细胞之间H2O2水平的差异，从而突出其在肿瘤研究和药物筛选中的潜力。本研究为研究肿瘤相关氧化应激和评估治疗反应提供了一个高效可靠的平台。

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引用次数: 0

Self-driven microfluidic chip with integrated lateral and vertical flow assays for dual-readout point-of-care testing 自驱动微流控芯片集成横向和垂直流动分析双读出点护理测试。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-25 DOI: 10.1016/j.bios.2025.118247

Qifan Zhou , Yu Lu , Miaomiao Li , Chuang Qi , Xiaoxuan Sun , Hui Zhang , Jinliang Sheng , Cheng Chen , Xiaoyi Lv , Xiangwei Zhao

Rapid and sensitive detection of biomarkers is pivotal for disease prevention and control in point-of-care testing (POCT). Traditional nitrocellulose (NC) membrane-based lateral flow assay (LFA) often cause suboptimal sensitivity and poor uniformity by disordered porous structure. Herein, we propose a self-driven microfluidic chip with integrated lateral and vertical flow assays (VFA), termed the SeDM-LV chip. By replacing the test line in LFA with an anodized aluminum oxide (AAO) membrane and incorporating surface-enhanced Raman scattering (SERS), the chip enables rapid, highly sensitive, and reproducible detection of target biomarkers. Taking Interleukin 6 (IL-6) as a proof of concept, the optimized chip demonstrates adequate detection performance, with naked-eye and SERS-based detection limits of 100 pg/mL and 0.95 pg/mL. The calibration curve exhibits a linear dynamic range spanning from 1 pg/mL to 1 μg/mL (R² = 0.972), while the one-step “sample-to-answer” protocol is completed only within 5 min. Chip evaluation revealed recoveries ranging from 92.1 % to 109 % in spiked healthy human serum for IL-6 quantification, alongside effective differentiate on between IL-6 abnormal (n = 8) and normal (n = 4) groups in clinical serum samples. Conceptually, this represents the first achievement of synergistic optimization of LFA and VFA, overcoming the long-standing trade-off between speed, sensitivity and user-friendliness in conventional POCT. The integrated chip features portability, simplicity, low cost and high expandability, rendering it highly significant for primary care screening and on-site detection during public health emergencies.

快速和灵敏的生物标志物检测是疾病预防和控制的关键在护理点检测（POCT）。传统的基于硝化纤维素（NC）膜的横向流动分析（LFA）由于多孔结构的无序性，往往导致灵敏度不理想和均匀性差。在此，我们提出了一种集成横向和垂直流动分析（VFA）的自驱动微流控芯片，称为SeDM-LV芯片。通过用阳极氧化铝（AAO）膜取代LFA中的测试线，并结合表面增强拉曼散射（SERS），该芯片能够快速，高灵敏度和可重复性地检测目标生物标志物。以白细胞介素6 （Interleukin 6, IL-6）为概念验证，优化后的芯片具有足够的检测性能，裸眼和基于sers的检测限分别为100 pg/mL和0.95 pg/mL。校准曲线线性动态范围为1 pg/mL ~ 1 μg/mL (R2 = 0.972)，而“从样品到答案”一步法仅需5 min即可完成。芯片评估显示，在加标的健康人血清中，IL-6定量的回收率为92.1%至109%，同时在临床血清样本中有效区分IL-6异常（n = 8）和正常（n = 4）组。从概念上讲，这代表了LFA和VFA协同优化的第一个成就，克服了传统POCT中长期存在的速度、灵敏度和用户友好性之间的权衡。该集成芯片具有便携、简单、成本低、可扩展性强等特点，对突发公共卫生事件中的初级保健筛查和现场检测具有重要意义。

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引用次数: 0