Agung Sri Damayanti, D. Lestari, Febrina Artauli Siahaan
Five endangered plant species ( Parmentrera cereifera Seem., Santalum album L., Dillenia philipphinensis Rolfe, Reutealis trisperma (Blanco) Airy Shaw, Joannesia princeps Vell.) with recalcitrant seeds were studied to conserve them. The study aimed to determine the viability of the seeds with different storage treatments and their germination behavior. This study was conducted at the seed bank of Purwodadi Botanic Garden by storing seeds for three months, during which the seeds were sown to test their viability every month. Seed germination was done in the green houses at Purwodadi Botanic Garden with three replicates of three seeds to observe their growth. The germination percentage indicates the level of viability of the seeds after being stored for three months. Seeds of D. philippinensis are capable to germinate after being stored at room temperature for one month (55%) and cold temperature for two months (41.67%). Seeds of P. cereifera . can germinate after three months of storage at cold temperature (70%) or room temperature (56.67%). Seeds of S. album were able to survive in cold temperature and room temperature storage despite of low germination percentage (36.67% and 20%). Similar to J. princeps and R. trisperma , the seeds of R. trisperma survives after two months of storage. Storage period and storage temperature greatly affect seed viability of the five endangered plants species.
{"title":"PENGUJIAN DAYA SIMPAN DAN VIABILITAS LIMA JENIS BIJI TUMBUHAN LANGKA: Parmentiera cereifera Seem., Santalum album L., Dillenia philippinensis Rolfe, Reutealis trisperma (Blanco) Airy Shaw dan Joannesia princeps Vell","authors":"Agung Sri Damayanti, D. Lestari, Febrina Artauli Siahaan","doi":"10.14203/bkr.v20i2.416","DOIUrl":"https://doi.org/10.14203/bkr.v20i2.416","url":null,"abstract":"Five endangered plant species ( Parmentrera cereifera Seem., Santalum album L., Dillenia philipphinensis Rolfe, Reutealis trisperma (Blanco) Airy Shaw, Joannesia princeps Vell.) with recalcitrant seeds were studied to conserve them. The study aimed to determine the viability of the seeds with different storage treatments and their germination behavior. This study was conducted at the seed bank of Purwodadi Botanic Garden by storing seeds for three months, during which the seeds were sown to test their viability every month. Seed germination was done in the green houses at Purwodadi Botanic Garden with three replicates of three seeds to observe their growth. The germination percentage indicates the level of viability of the seeds after being stored for three months. Seeds of D. philippinensis are capable to germinate after being stored at room temperature for one month (55%) and cold temperature for two months (41.67%). Seeds of P. cereifera . can germinate after three months of storage at cold temperature (70%) or room temperature (56.67%). Seeds of S. album were able to survive in cold temperature and room temperature storage despite of low germination percentage (36.67% and 20%). Similar to J. princeps and R. trisperma , the seeds of R. trisperma survives after two months of storage. Storage period and storage temperature greatly affect seed viability of the five endangered plants species.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130482281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Yudaputra, R. N. Zulkarnaen, A. N. Rachmadiyanto, J. Witono, I. P. Astuti
Hydriastele beguinii (Burret) W.J. Baker & Loo is an endemic palm from Moluccas Island. The palm is commonly used as an ornamental plant because of its unique characteristics. Reproduction is an important part of plant life’s cycles to maintain and sustain their existence. The reproduction ability of H. beguinii is relatively low in its natural habitat, therefore studies on its reproduction aspect are required. The main objective of this study is to assess reproduction phenology of three living collections of H. beguinii at Bogor Botanic Gardens. Three individuals of H. beguinii at initiation phase were selected. There are several main observations that were carried out on this study, namely the duration of every phase, the morphological changes in every phase, the biotic and abiotic factors that affect reproduction phenology of H. beguinii . According to the observation, the initiation phase took 12-16 days, bud towards anthesis phase took 8-10 days, anthesis phase took 14-16 days, and young fruits to maturity phase took 110-124 days. The result of this study showed that each of reproduction phenology phase has a different time period and morphological changes. In addition, reproduction phenology phases were not directly affected by abiotic factors. Trigona laevicep was an insect visitor that was suspected as a pollinator of H . beguinii .
{"title":"REPRODUCTION PHENOLOGY OF Hydriastele beguinii (Buret) W.J. Baker & Loo IN BOGOR BOTANIC GARDENS","authors":"A. Yudaputra, R. N. Zulkarnaen, A. N. Rachmadiyanto, J. Witono, I. P. Astuti","doi":"10.14203/bkr.v20i2.420","DOIUrl":"https://doi.org/10.14203/bkr.v20i2.420","url":null,"abstract":"Hydriastele beguinii (Burret) W.J. Baker & Loo is an endemic palm from Moluccas Island. The palm is commonly used as an ornamental plant because of its unique characteristics. Reproduction is an important part of plant life’s cycles to maintain and sustain their existence. The reproduction ability of H. beguinii is relatively low in its natural habitat, therefore studies on its reproduction aspect are required. The main objective of this study is to assess reproduction phenology of three living collections of H. beguinii at Bogor Botanic Gardens. Three individuals of H. beguinii at initiation phase were selected. There are several main observations that were carried out on this study, namely the duration of every phase, the morphological changes in every phase, the biotic and abiotic factors that affect reproduction phenology of H. beguinii . According to the observation, the initiation phase took 12-16 days, bud towards anthesis phase took 8-10 days, anthesis phase took 14-16 days, and young fruits to maturity phase took 110-124 days. The result of this study showed that each of reproduction phenology phase has a different time period and morphological changes. In addition, reproduction phenology phases were not directly affected by abiotic factors. Trigona laevicep was an insect visitor that was suspected as a pollinator of H . beguinii .","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132955012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aims of this research are to understand flowering phenology of Areca vestiaria from highland and lowland to understand its flowering-fruiting productivity and the relation between its vegetative and reproductive growth. The study was conducted by observing four collections A. vestiaria from North and Central Sulawesi at Eka Karya Botanic Gardens. The study used a descriptive method by observing a flowering period including initiation, buds to anthesis, anthesis, young fruit and ripen fruit. The time required from beginning of flower until ripe fruit is 322 days for highland accession and 337 days for lowland accession. The average proportion of female flowers and male flowers in A. vestiaria is only 0.25. The number of female flowers of A. vestiaria from lowland accession is less than of the highland accession which is only 24.7%. The highland accession’s female flower ability to form young fruit is also bigger than that of the lowland accession (98.82% and 41.67% respectively). However, the highland accession’s sources to develop its young fruits into viable ripen fruits is lower than that of the lowland accessions (27.380% and 60%, respectively). There is a correlation between the height of the tree and the amount of flower produced (0.7) and there is no correlation between the diameter to the height and the amount of flowering. Seven species of insect were identified but pollinator insects are yet still unkown.
{"title":"FENOLOGI PEMBUNGAAN Areca vestiaria Giseke DI KEBUN RAYA EKA KARYA BALI","authors":"Siti Fatimah Hanum, D. Lestari","doi":"10.14203/BKR.V20I2.417","DOIUrl":"https://doi.org/10.14203/BKR.V20I2.417","url":null,"abstract":"The aims of this research are to understand flowering phenology of Areca vestiaria from highland and lowland to understand its flowering-fruiting productivity and the relation between its vegetative and reproductive growth. The study was conducted by observing four collections A. vestiaria from North and Central Sulawesi at Eka Karya Botanic Gardens. The study used a descriptive method by observing a flowering period including initiation, buds to anthesis, anthesis, young fruit and ripen fruit. The time required from beginning of flower until ripe fruit is 322 days for highland accession and 337 days for lowland accession. The average proportion of female flowers and male flowers in A. vestiaria is only 0.25. The number of female flowers of A. vestiaria from lowland accession is less than of the highland accession which is only 24.7%. The highland accession’s female flower ability to form young fruit is also bigger than that of the lowland accession (98.82% and 41.67% respectively). However, the highland accession’s sources to develop its young fruits into viable ripen fruits is lower than that of the lowland accessions (27.380% and 60%, respectively). There is a correlation between the height of the tree and the amount of flower produced (0.7) and there is no correlation between the diameter to the height and the amount of flowering. Seven species of insect were identified but pollinator insects are yet still unkown.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132814302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enggano Island is a small island in northwest Bengkulu, Sumatera. The mosses diversity of Enggano Island has ever been reported, unfortunately it has never been identified to species. The inventory of Enggano’s mosses reported 49 species comprising 27 genera and 12 families. Aerobryopsis wallichii (Brid.) M. Fleisch is a dominant species of mosses in study site. Five families consisting of 17 genera and 49 species are new records to Enggano’s mosses. Two species namely are Fissidens robinsonii Broth. and Syrrhopodon semilimber (Mitt.) Besch are recorded as new record to Enggano Island and probably are also found in main island, Sumatera.
{"title":"REKAMAN BARU TUMBUHAN LUMUT SEJATI DI PULAU ENGGANO, SUMATERA","authors":"F. I. Windadri, Dewi Rosalina","doi":"10.14203/BKR.V20I2.419","DOIUrl":"https://doi.org/10.14203/BKR.V20I2.419","url":null,"abstract":"Enggano Island is a small island in northwest Bengkulu, Sumatera. The mosses diversity of Enggano Island has ever been reported, unfortunately it has never been identified to species. The inventory of Enggano’s mosses reported 49 species comprising 27 genera and 12 families. Aerobryopsis wallichii (Brid.) M. Fleisch is a dominant species of mosses in study site. Five families consisting of 17 genera and 49 species are new records to Enggano’s mosses. Two species namely are Fissidens robinsonii Broth. and Syrrhopodon semilimber (Mitt.) Besch are recorded as new record to Enggano Island and probably are also found in main island, Sumatera.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"248 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133622164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Duku ( Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) from Kuantan Singingi Regency, Riau, known by local people as duku turak, has a unique oval betel nut-like neck, and tastes sweet similar to round duku. It has thick skin that is not easy to break, nor quickly rot. This duku was more preferred by local people than the round shaped duku known as duku gondok. However, the information about the potential of duku turak has not been known widely. The aim of this study was to compare morphology and isozyme characteristics of duku turak and gondok growing in Kuantan Singingi Regency based on morphological and isozyme characteristics. The result showed that turak and gondok can be distinguished based on morphological characteristics i.e tree, leave and fruit shape. Analysis of isozyme banding patterns used esterase isozyme which shows variation in the resulting patterns formed two bands. Acid phospatase (ACP) isozyme formed eight bands and aspartate aminotransferase (AAT) isozyme formed only one band. However, peroksidase isozyme showed no different variation in the resulting patterns. Similarity coefficient value based on morphology characters was about 0.15 – 0.74, while value of isozyme analysis was about 0.29 – 1.00 as well as the combination among morphology characters and isozyme were about 0.24 – 0.71. The results of this study provide characteristic information and taxonomic groupings that can be useful as a type improvement program that relies heavily on available genetic diversity
{"title":"STUDI KOMPARASI KARAKTER MORFOLOGI DAN ISOZIM DUKU Lansium parasiticum (Osbeck) K.C. Sahni & Bennet ASAL KABUPATEN KUANTAN SINGINGI, RIAU","authors":"A. Damayanti, Fitmawati Fitmawati, H. Herman","doi":"10.14203/BKR.V20I2.415","DOIUrl":"https://doi.org/10.14203/BKR.V20I2.415","url":null,"abstract":"Duku ( Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) from Kuantan Singingi Regency, Riau, known by local people as duku turak, has a unique oval betel nut-like neck, and tastes sweet similar to round duku. It has thick skin that is not easy to break, nor quickly rot. This duku was more preferred by local people than the round shaped duku known as duku gondok. However, the information about the potential of duku turak has not been known widely. The aim of this study was to compare morphology and isozyme characteristics of duku turak and gondok growing in Kuantan Singingi Regency based on morphological and isozyme characteristics. The result showed that turak and gondok can be distinguished based on morphological characteristics i.e tree, leave and fruit shape. Analysis of isozyme banding patterns used esterase isozyme which shows variation in the resulting patterns formed two bands. Acid phospatase (ACP) isozyme formed eight bands and aspartate aminotransferase (AAT) isozyme formed only one band. However, peroksidase isozyme showed no different variation in the resulting patterns. Similarity coefficient value based on morphology characters was about 0.15 – 0.74, while value of isozyme analysis was about 0.29 – 1.00 as well as the combination among morphology characters and isozyme were about 0.24 – 0.71. The results of this study provide characteristic information and taxonomic groupings that can be useful as a type improvement program that relies heavily on available genetic diversity","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125505098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pulau Flores adalah salah satu dari empat pulau besar di Provinsi Nusa Tenggara Timur. Pulau Flores tercatat masih mempunyai kawasan hutan yang menyimpan keanekaragam sumber daya hayati tumbuhan cukup banyak. Namun informasi mengenai data kekayaan sumber daya hayati tumbuhannya masih sangat sedikit. Ekspedisi Negara Kesatuan Republik Indonesia (NKRI) subkorwil Enam Ende, sebagai salah satu subkorwil yang bertugas mengungkap kekayaan sumber daya hayati tumbuhan di Pulau Flores berhasil menginventarisasi dan menemukan dua jenis Amorphophallus dari suku Araceae, yaitu adalah Amorphophallus muelerii Blume dan A. paeoniifolius (Dennst.) Nicolson. Tulisan ini menyajikan deskripsi dari kedua jenis tersebut.
{"title":"Amorphophallus muelleri Blume dan Amorphophallus paeoniifolius (Dennst.) Nicolson dari Pulau Flores","authors":"Inggit Puji Astuti, Sayyidah Fatchiyyah, Dwi Ariyuni, Nurjannah Yuanisa Ruliyanti, Gae Piyus, Blasius Ndahu, Dian Andi Yudha, Dian Mustofa","doi":"10.14203/BKR.V20I1.404","DOIUrl":"https://doi.org/10.14203/BKR.V20I1.404","url":null,"abstract":"Pulau Flores adalah salah satu dari empat pulau besar di Provinsi Nusa Tenggara Timur. Pulau Flores tercatat masih mempunyai kawasan hutan yang menyimpan keanekaragam sumber daya hayati tumbuhan cukup banyak. Namun informasi mengenai data kekayaan sumber daya hayati tumbuhannya masih sangat sedikit. Ekspedisi Negara Kesatuan Republik Indonesia (NKRI) subkorwil Enam Ende, sebagai salah satu subkorwil yang bertugas mengungkap kekayaan sumber daya hayati tumbuhan di Pulau Flores berhasil menginventarisasi dan menemukan dua jenis Amorphophallus dari suku Araceae, yaitu adalah Amorphophallus muelerii Blume dan A. paeoniifolius (Dennst.) Nicolson. Tulisan ini menyajikan deskripsi dari kedua jenis tersebut.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"289 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122404827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Latifah, Melani Kurnia Riswati, Elizabeth Handini, Hary Wawangningrum
Rafflesia species are holoparasites that are threatened by habitat loss. The Center for Plant Conservation Botanic Gardens–LIPI has been pioneering ex situ conservation of the plants using grafting methods. This R. arnoldii and R. patma ex situ conservation effort using seed innoculation has proven to be a challenge. Studies on viability testing of seeds aims to ensure viable seeds for supporting ex situ seed innoculation conservation programs. This research aimed to: (1) observe the morphological characters of the seeds and (2) determine a method for viability testing. Morphological characters of the seeds were observed under a binocular microscope. The viability test using bioassay procedures included: (1) reagent preparation; (2) pre–staining; (3) embryo exposure; (4) staining, and (5) assessment. The research resulted in: (1) morphological characteristics of R. arnoldii seeds, 2–3–times larger than those of R. patma, and that seeds of the two species have distinct testas, and (2) techniques for bioassay viability testing of Rafflesia seeds, i.e. the viability test resulted in an estimated viability percentage of 78.75 ± 4.75 % ( R. arnoldii seeds) and 93.29 2.67 % ( R. patma seeds).
{"title":"Viability Tests On The Seeds Of Rafflesia Arnoldii R.br. And R. Patma Blume","authors":"D. Latifah, Melani Kurnia Riswati, Elizabeth Handini, Hary Wawangningrum","doi":"10.14203/bkr.v0i0.123","DOIUrl":"https://doi.org/10.14203/bkr.v0i0.123","url":null,"abstract":"Rafflesia species are holoparasites that are threatened by habitat loss. The Center for Plant Conservation Botanic Gardens–LIPI has been pioneering ex situ conservation of the plants using grafting methods. This R. arnoldii and R. patma ex situ conservation effort using seed innoculation has proven to be a challenge. Studies on viability testing of seeds aims to ensure viable seeds for supporting ex situ seed innoculation conservation programs. This research aimed to: (1) observe the morphological characters of the seeds and (2) determine a method for viability testing. Morphological characters of the seeds were observed under a binocular microscope. The viability test using bioassay procedures included: (1) reagent preparation; (2) pre–staining; (3) embryo exposure; (4) staining, and (5) assessment. The research resulted in: (1) morphological characteristics of R. arnoldii seeds, 2–3–times larger than those of R. patma, and that seeds of the two species have distinct testas, and (2) techniques for bioassay viability testing of Rafflesia seeds, i.e. the viability test resulted in an estimated viability percentage of 78.75 ± 4.75 % ( R. arnoldii seeds) and 93.29 2.67 % ( R. patma seeds).","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115318323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paphiopedilum supardii Braem & Loeb is one of Slipper Orchids from Southeast Kalimantan, which is included in Appendix I of CITES. It is a rare orchid with restricted habitat endemic to Kalimantan, found growing at altitude of 600-900 m above sea level. In Indonesia, this species is categorized as the priority species for Conservation. The aim of this research is to conserve this orchid species through seed storage method in deep freezer at temperature of -20 o C then sowing seed by in vitro propagation. Seed viability test was carried out in 0 day storage (without storage) as a control, then an interval of 3 months, 6 months, 9 months and still will continue until no longer able to germinate. Four different media cultures were used to test the germination in vitro culture. The result showed that the seeds of Paphiopedilum supardii were able to germinate in 4 different media i.e. Knudson C with micro nutrient (KCA), modified Knudson C (KC), modified Vacin & Went (VW) and modified Hyponex fertilizer (HS). Subculture for multiplication and rooting phase, based on the best germination medium. The germination test showed that Knudson’C medium with micronutrient (KCA) resulted on the best performance of green protocorm , while the other media showed the brown swollen protocorm . Unfortunately, KCA gave the lowest percentage of germination. Furthermore subculture for multiplication and rooting phase was the best in Knudson C medium with addition of micronutrient (KCA) and coconut water.
supardii Braem & Loeb Paphiopedilum supardii Braem & Loeb是产于加里曼丹东南部的拖鞋兰之一，被列入CITES附录I。它是一种稀有的兰花，在加里曼丹特有的栖息地受到限制，生长在海拔600-900米的地方。在印度尼西亚，该物种被列为优先保护物种。本研究的目的是在-20℃的低温冷库中对该兰花进行种子保存，然后进行离体繁殖播种。以0 d贮藏(不贮藏)为对照进行种子活力试验，然后间隔3个月、6个月、9个月，直至不能发芽为止。采用4种不同培养基进行离体萌发试验。结果表明，suppardii Paphiopedilum种子在Knudson C +微量养分(KCA)、改良Knudson C (KC)、改良Vacin & go (VW)和改良Hyponex肥(HS) 4种不同培养基上均能萌发。根据最佳发芽培养基进行繁殖和生根期的继代培养。发芽试验表明，添加微量元素(KCA)的Knudson’c培养基的原球茎绿色表现最好，而其他培养基的原球茎呈褐色肿胀。不幸的是，KCA的发芽率最低。在添加微量元素(KCA)和椰子水的Knudson C培养基上继代繁殖和生根期效果最好。
{"title":"Konservasi Paphiopedilum supardii Braem& Loeb dengan Metode Penyimpanan Biji dan Perbanyakan secara In Vitro","authors":"E. Handini, D. M. Puspitaningtyas, R. V. Garvita","doi":"10.14203/BKR.V19I2.180","DOIUrl":"https://doi.org/10.14203/BKR.V19I2.180","url":null,"abstract":"Paphiopedilum supardii Braem & Loeb is one of Slipper Orchids from Southeast Kalimantan, which is included in Appendix I of CITES. It is a rare orchid with restricted habitat endemic to Kalimantan, found growing at altitude of 600-900 m above sea level. In Indonesia, this species is categorized as the priority species for Conservation. The aim of this research is to conserve this orchid species through seed storage method in deep freezer at temperature of -20 o C then sowing seed by in vitro propagation. Seed viability test was carried out in 0 day storage (without storage) as a control, then an interval of 3 months, 6 months, 9 months and still will continue until no longer able to germinate. Four different media cultures were used to test the germination in vitro culture. The result showed that the seeds of Paphiopedilum supardii were able to germinate in 4 different media i.e. Knudson C with micro nutrient (KCA), modified Knudson C (KC), modified Vacin & Went (VW) and modified Hyponex fertilizer (HS). Subculture for multiplication and rooting phase, based on the best germination medium. The germination test showed that Knudson’C medium with micronutrient (KCA) resulted on the best performance of green protocorm , while the other media showed the brown swollen protocorm . Unfortunately, KCA gave the lowest percentage of germination. Furthermore subculture for multiplication and rooting phase was the best in Knudson C medium with addition of micronutrient (KCA) and coconut water.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121625076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asvic Helida, Ervizal A. M. Zuhud, H. Hardjanto, Yudhi Purwanto, Agus Hikmat
Ethnobotany is required to study the concepts of local knowledge about the plants which are the result of the development of the culture of a community, while conservation is an effort to maintain the sustainability of natural resources through protection, preservation and wise use. Ethnobotany can be used as an indicator for successful forest resources management. The objective of this study are to identify the local knowledge of Kerinci community associated to the plant and to analyze the level of knowledge and retention in ethnobotany. The study was conducted in three locations namely Dusun Lempur Baru, Dusun Ulu Jernih and Dusun Lama Tamiai in Kerinci District, Jambi Province. The research was from October 2013 to October 2014. Qualitative research approach with participant observation and quantitative methods using questionnaires with 30 respondents in each location. The results showed that the level of knowledge Kerinci community in ethnobotany is at the medium level ( MGJ = 0.625 ). There are differences in the level of knowledge among the three study sites. There is a decline of the knowledge with the annual average annual change is CA < 0.1. Keywords : Change of ethnobotany knowledge, Kerinci community, retention ethnobotany knowledge
民族植物学需要研究当地植物知识的概念，这些概念是社区文化发展的结果，而保护是通过保护，保存和明智利用来维持自然资源的可持续性的努力。民族植物学可以作为成功管理森林资源的一个指标。本研究的目的是确定Kerinci社区与该植物相关的当地知识，并分析民族植物学知识和保留水平。该研究在占碑省Kerinci区的Dusun Lempur Baru、Dusun Ulu Jernih和Dusun Lama Tamiai三个地点进行。研究时间为2013年10月至2014年10月。定性研究方法采用参与观察和定量方法，在每个地点使用30名受访者的问卷调查。结果表明:云南喀喇木铃群落在民族植物学方面的知识水平处于中等水平(MGJ = 0.625);三个研究地点的知识水平存在差异。知识呈下降趋势，年平均变化量CA < 0.1。关键词:民族植物学知识变迁，克林芝社区，民族植物学知识留存
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