Pub Date : 2024-01-31DOI: 10.21608/mjab.2024.346339
R. Rashed, .. M. A. M. Hammam, S. A. El-Kadousy, S. M. Abd El-Gawad
{"title":"Chemical Studies On Some Nanotechnology Applications.","authors":"R. Rashed, .. M. A. M. Hammam, S. A. El-Kadousy, S. M. Abd El-Gawad","doi":"10.21608/mjab.2024.346339","DOIUrl":"https://doi.org/10.21608/mjab.2024.346339","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"416 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140474896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.21608/mjab.2024.254873.1014
Eman Ali El-feky, M. Hammam, A. Sakr, ,. M. Abozid
: Fats are an essential component of human diets and their types greatly affects public health, and some of them may cause many diseases, such as cardiovascular diseases and fatty liver. Since sunflower, flaxseed and olive oils are among the oils commonly used in nutrition in Egypt, the study was conducted to compare the components of these oils and their nutritional value to evaluate them. The fatty acid components of these oils were estimated, and the sterols present in each oil were estimated using the Gas Chromatograph (GC). The physical and chemical properties of each oil were also estimated (specific gravity, refractive index, color, oxidative stability index, saponification value, iodine value, acid value, ester value, peroxide value and % unsaponifiable matter). Linoleic acid was the largest component in sunflower oil (53.33%); oleic acid was the largest one in olive oil (66.65%), while alpha-linolenic acid was the largest component in flaxseed oil (55.34%). For sterols, β-sitosterol (44.86%), stigmasterol (20.33%), campesterol (11.68%), isofucosterol (9.54%) components were the largest in sunflower oil. Squalene (64.14%) and β-sitosterol (16.78%) were the largest sterols in olive oil. While β-sitosterol (28.24%), campesterol (16.37%) and cycloartenyl acetate (12.42%) were the largest sterols in flaxseed oil. The results clearly show a high content of sunflower oil of omega-6 fatty acids, while flaxseed oil was distinguished by its high content of omega-3 fatty acids, and olive oil was the highest in its content of omega-9 fatty acids. Therefore, these oils are well suited for use in nutrition, and more studies are needed to reach the best ratios of the mixture of these oils in biological experiments.
{"title":"EVALUATION OF SUNFLOWER, FLAXSEED AND OLIVE OILS IN TERMS OF CHEMICAL COMPOSITION AND THEIR PHYSICAL AND CHEMICAL PROPERTIES","authors":"Eman Ali El-feky, M. Hammam, A. Sakr, ,. M. Abozid","doi":"10.21608/mjab.2024.254873.1014","DOIUrl":"https://doi.org/10.21608/mjab.2024.254873.1014","url":null,"abstract":": Fats are an essential component of human diets and their types greatly affects public health, and some of them may cause many diseases, such as cardiovascular diseases and fatty liver. Since sunflower, flaxseed and olive oils are among the oils commonly used in nutrition in Egypt, the study was conducted to compare the components of these oils and their nutritional value to evaluate them. The fatty acid components of these oils were estimated, and the sterols present in each oil were estimated using the Gas Chromatograph (GC). The physical and chemical properties of each oil were also estimated (specific gravity, refractive index, color, oxidative stability index, saponification value, iodine value, acid value, ester value, peroxide value and % unsaponifiable matter). Linoleic acid was the largest component in sunflower oil (53.33%); oleic acid was the largest one in olive oil (66.65%), while alpha-linolenic acid was the largest component in flaxseed oil (55.34%). For sterols, β-sitosterol (44.86%), stigmasterol (20.33%), campesterol (11.68%), isofucosterol (9.54%) components were the largest in sunflower oil. Squalene (64.14%) and β-sitosterol (16.78%) were the largest sterols in olive oil. While β-sitosterol (28.24%), campesterol (16.37%) and cycloartenyl acetate (12.42%) were the largest sterols in flaxseed oil. The results clearly show a high content of sunflower oil of omega-6 fatty acids, while flaxseed oil was distinguished by its high content of omega-3 fatty acids, and olive oil was the highest in its content of omega-9 fatty acids. Therefore, these oils are well suited for use in nutrition, and more studies are needed to reach the best ratios of the mixture of these oils in biological experiments.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"27 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140521238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.21608/mjab.2024.258618.1015
mostafa hamam, samir mohamed, salah mansour, R. Rashed
,
,
{"title":"BIOCHEMICAL AND BIOLOGICAL STUDIES ON ZINC OXIDE NANOPARTICLES","authors":"mostafa hamam, samir mohamed, salah mansour, R. Rashed","doi":"10.21608/mjab.2024.258618.1015","DOIUrl":"https://doi.org/10.21608/mjab.2024.258618.1015","url":null,"abstract":",","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"75 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140523554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01DOI: 10.21608/mjab.2023.241179.1013
M. A Hammam,, F. M El-Shouni, S. M El-Sayed,, A. M.M Ali
: The present study was designed to investigate the chemical composition, determination the phenolic contents in plant extracts and studying the cytotoxic effects of plant seeds against land snail. The plants under study were Portulaca oleracea and Cichorium intybus. Our results could be summarized that Portulaca oleracea (purslane) contain ash, fiber, crude lipids, protein and carbohydrate 2.15, 33, 26, 11.6 and 60.25 % and Cichorium intybus (chicory) contain 1.65, 35, 19, 10 and 69.35 % respectively. Purslane contain 13 phenolic compounds, meanwhile chicory contain 16 phenolic compounds, the main compounds in purslane seed extract were coumaric acid (129.62 μg/ml) and coffeic acid (108.98 μg/ml) and main compounds in chicory were naringenin (3631.04 μg/ml) and chlorogenic acid (2395.18 μg/ml). Results showed cytotoxic effects of extracts of purslane and chicory seeds on the land snails iv stage, whereas increases the extract concentration from (25 to 100%) led to decrease the egg ms/snail, egg no./ms, egg no. / snail, Inc. period (day) and hatch rate.
{"title":"CYTOTOXIC EFFECTS OF PORTULACA OLERACEA AND CICHORIUM INTYBUS SEEDS ON LAND SNAIL MONACHA CARTUSIANA","authors":"M. A Hammam,, F. M El-Shouni, S. M El-Sayed,, A. M.M Ali","doi":"10.21608/mjab.2023.241179.1013","DOIUrl":"https://doi.org/10.21608/mjab.2023.241179.1013","url":null,"abstract":": The present study was designed to investigate the chemical composition, determination the phenolic contents in plant extracts and studying the cytotoxic effects of plant seeds against land snail. The plants under study were Portulaca oleracea and Cichorium intybus. Our results could be summarized that Portulaca oleracea (purslane) contain ash, fiber, crude lipids, protein and carbohydrate 2.15, 33, 26, 11.6 and 60.25 % and Cichorium intybus (chicory) contain 1.65, 35, 19, 10 and 69.35 % respectively. Purslane contain 13 phenolic compounds, meanwhile chicory contain 16 phenolic compounds, the main compounds in purslane seed extract were coumaric acid (129.62 μg/ml) and coffeic acid (108.98 μg/ml) and main compounds in chicory were naringenin (3631.04 μg/ml) and chlorogenic acid (2395.18 μg/ml). Results showed cytotoxic effects of extracts of purslane and chicory seeds on the land snails iv stage, whereas increases the extract concentration from (25 to 100%) led to decrease the egg ms/snail, egg no./ms, egg no. / snail, Inc. period (day) and hatch rate.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"121 1-2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135714865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/mjab.2023.321680
Mohamed Hamed Mohamed Ismaiel, A. M. El-Zanaty, Kh. S. Mohamed
: Many Trichoderma aggregate species are of economic interest because of their ability to produce hydrolytic enzymes. In this study , twenty isolates of Trichoderma were obtained from lignocellulosic agriculture wastes-rich soil collected from different Egyptian locations (Sadat, Tala, Abo Hamad, Belbeis, Zagazig, Mansoura, Belqas, Kafer-Elshikh, Bella, Tanta, Borg El Arab, Banha, Kafr Shoker, Qalyoub, Shebien Elqanater, Damanhur, Abu al-Matamir, Damietta, Kafr al-Battikh, and Kafr Saad). These isolates were first identified morphologically based on conidiophore branching type and conidium morphology. Seven isolates were found to belong to Trichoderma Viride : TM4, TM5, TM9, TM13, TM16, TM29, and TM42; four isolates classified as Trichoderma Koningii : TM6, TM8, TM18 and, TM19 and nine isolates were identified as Trichoderma Harizianum : TM23, TM30, TM31, TM33, TM35, TM36, TM41, TM44, and TM45. Molecular
{"title":"Detection of some Trichoderma genes for biodegradation of cellulosic wastes","authors":"Mohamed Hamed Mohamed Ismaiel, A. M. El-Zanaty, Kh. S. Mohamed","doi":"10.21608/mjab.2023.321680","DOIUrl":"https://doi.org/10.21608/mjab.2023.321680","url":null,"abstract":": Many Trichoderma aggregate species are of economic interest because of their ability to produce hydrolytic enzymes. In this study , twenty isolates of Trichoderma were obtained from lignocellulosic agriculture wastes-rich soil collected from different Egyptian locations (Sadat, Tala, Abo Hamad, Belbeis, Zagazig, Mansoura, Belqas, Kafer-Elshikh, Bella, Tanta, Borg El Arab, Banha, Kafr Shoker, Qalyoub, Shebien Elqanater, Damanhur, Abu al-Matamir, Damietta, Kafr al-Battikh, and Kafr Saad). These isolates were first identified morphologically based on conidiophore branching type and conidium morphology. Seven isolates were found to belong to Trichoderma Viride : TM4, TM5, TM9, TM13, TM16, TM29, and TM42; four isolates classified as Trichoderma Koningii : TM6, TM8, TM18 and, TM19 and nine isolates were identified as Trichoderma Harizianum : TM23, TM30, TM31, TM33, TM35, TM36, TM41, TM44, and TM45. Molecular","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/mjab.2023.312767
Elham Abdelrahman Gomaa Basiouny, M. M. Abozid, A. A. A. A. A. A. Abdelaal, M. M. S. Asker
: Bees play a vital role in pollinating different plants, which will greatly contribute to the development of plant wealth. They also produce honey, which is one of the most important natural foods that are characterized by many nutritional benefits, as well as many benefits to human health, strengthening immunity and resistance to many diseases. Therefore, diseases affecting bees represent major challenges in the field of agriculture in general and the field of bees particularly. The AFB and EFB diseases are the most affecting disorders of bees and the most destructive to the hives. So that, searching for new ways to resist them is of a great interest at the Egyptian agricultural sector without resorting to antibiotics
{"title":"Biochemical effects of some plant extracts on combating some common honeybee diseases in Menoufia governorate","authors":"Elham Abdelrahman Gomaa Basiouny, M. M. Abozid, A. A. A. A. A. A. Abdelaal, M. M. S. Asker","doi":"10.21608/mjab.2023.312767","DOIUrl":"https://doi.org/10.21608/mjab.2023.312767","url":null,"abstract":": Bees play a vital role in pollinating different plants, which will greatly contribute to the development of plant wealth. They also produce honey, which is one of the most important natural foods that are characterized by many nutritional benefits, as well as many benefits to human health, strengthening immunity and resistance to many diseases. Therefore, diseases affecting bees represent major challenges in the field of agriculture in general and the field of bees particularly. The AFB and EFB diseases are the most affecting disorders of bees and the most destructive to the hives. So that, searching for new ways to resist them is of a great interest at the Egyptian agricultural sector without resorting to antibiotics","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132235923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-06DOI: 10.21608/mjab.2023.215374.1009
Mohamed Moheb
{"title":"Impact of Psidium guajava and Mangifera indica leaves on rats feed on high fat diet","authors":"Mohamed Moheb","doi":"10.21608/mjab.2023.215374.1009","DOIUrl":"https://doi.org/10.21608/mjab.2023.215374.1009","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115191136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.21608/mjab.2023.314612
{"title":"Biochemical Studies on Some Plant Extracts and their Effect on Blood Lipids Level","authors":"","doi":"10.21608/mjab.2023.314612","DOIUrl":"https://doi.org/10.21608/mjab.2023.314612","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135143540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-09DOI: 10.21608/mjab.2023.202737.1007
S. Khaled, ,. M. A. Hammam, F. El-Shouny, S. El-Sayed, M. Aly-Aldin
: The present study was designed to investigate the phenolic contents in green tea, to evaluate the antioxidant activity of plant extract and to studying the potential effect of green tea extract to increase protective effect against oxidative stress, and study the effect of plant on chemical properties of oil used in cupcakes. The main component of such green tea was methyle gallate, the extracts under study was found to have antioxidant activity in vitro and in vivo, green tea methanol extract recorded 0.645 at concentration of 100µg/ml in reducing power assay. In DPPH assay green tea methanol extract recorded inhibition percent of 90.4% at concentration of 100 µg/ml comparing with ascorbic acid 97.7%. Green tea have biological effect on liver functions (ALT and AST activity, ALB and TP levels), kidney functions (urea and creatinine levels) and antioxidant markers (CAT activity and MDA level). The treatment with green tea extracts led to reduction in ALT and AST activity, and an increase in total protein and albumin levels accompanied by reduction in creatinine and urea levels, meanwhile treatment with H 2 O 2 caused an elevation in CAT activity and MDA level but treatment with green tea extracts affected this elevation, also green tea effected on food technology during storage period of cupcakes and effected on the acid value and peroxide value of cupcakes oil under study.
本研究旨在研究绿茶中酚类物质的含量，评价植物提取物的抗氧化活性，研究绿茶提取物对抗氧化应激的潜在保护作用，研究植物对纸杯蛋糕油化学性质的影响。该绿茶的主要成分为没食子酸甲酯，所研究的提取物具有体外和体内抗氧化活性，绿茶甲醇提取物在浓度为100µg/ml时还原力测定为0.645。在DPPH实验中，绿茶甲醇提取物在100µg/ml浓度下的抑制率为90.4%，而抗坏血酸的抑制率为97.7%。绿茶对肝功能(ALT和AST活性、ALB和TP水平)、肾功能(尿素和肌酐水平)和抗氧化指标(CAT活性和MDA水平)均有生物学效应。绿茶提取物治疗导致降低ALT和AST活性,增加血清总蛋白和白蛋白水平伴随着降低肌酐和尿素的水平,与此同时治疗H 2 O 2导致猫活性和MDA水平但海拔绿茶提取物治疗这个海拔高度的影响,也影响绿茶食品技术存储期间的蛋糕和影响下蛋糕油的酸值和过氧化值研究。
{"title":"Biochemical and technological studies on green tea","authors":"S. Khaled, ,. M. A. Hammam, F. El-Shouny, S. El-Sayed, M. Aly-Aldin","doi":"10.21608/mjab.2023.202737.1007","DOIUrl":"https://doi.org/10.21608/mjab.2023.202737.1007","url":null,"abstract":": The present study was designed to investigate the phenolic contents in green tea, to evaluate the antioxidant activity of plant extract and to studying the potential effect of green tea extract to increase protective effect against oxidative stress, and study the effect of plant on chemical properties of oil used in cupcakes. The main component of such green tea was methyle gallate, the extracts under study was found to have antioxidant activity in vitro and in vivo, green tea methanol extract recorded 0.645 at concentration of 100µg/ml in reducing power assay. In DPPH assay green tea methanol extract recorded inhibition percent of 90.4% at concentration of 100 µg/ml comparing with ascorbic acid 97.7%. Green tea have biological effect on liver functions (ALT and AST activity, ALB and TP levels), kidney functions (urea and creatinine levels) and antioxidant markers (CAT activity and MDA level). The treatment with green tea extracts led to reduction in ALT and AST activity, and an increase in total protein and albumin levels accompanied by reduction in creatinine and urea levels, meanwhile treatment with H 2 O 2 caused an elevation in CAT activity and MDA level but treatment with green tea extracts affected this elevation, also green tea effected on food technology during storage period of cupcakes and effected on the acid value and peroxide value of cupcakes oil under study.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"2005 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125824124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-01DOI: 10.21608/mjab.2023.212610.1008
E. Basiouny, ahmed abdakaway, Mohsen S. Asker, Medhat Mostafa Abozid
: The problem of oxidation and free radicals is one of the factors that are very dangerous to human health, which requires searching for safe natural compounds to be included in the diet and work to combat free radicals. The aim of this study was to evaluate the antioxidant effect of essential oils of basil, thyme and lemongrass by two different methods (FRAP and DPPH) as well as to identify the active ingredients of each oil by GCMS. The results of the fractionation on the GCMS showed that the main components of the essential oils were as follows: basil (L-linalool 59.02% and 1,8-cineole 9.52%), thyme (Thymol 48.06%and p-cymene 38.57%), and lemongrass (Geranial 47.59% and neral 36.27%). In the FRAP method, essential oil of basil was the strongest as an antioxidant (2.35± 0.61 mMol Fe +2 /g), followed by essential oil of thyme (1.96± 0.42 mMol Fe +2 /g), followed by essential oil of lemongrass by a large margin (0.93± 0.21 mMol Fe +2 /g). Whereas, when studying the antioxidant activity by the DPPH method, the results showed through comparison with IC 50 values that, basil essential oil was the strongest antioxidant (IC 50 =144.78 ± 7.19 μg/ml), followed by lemongrass essential oil (IC 50 =170.05 ± 6.97 μg/ml) and finally thyme essential oil (IC 50 =183.45 ± 7.19 μg/ml), w ith a slight difference. The results clearly indicated that the essential oil of basil was the best as an antioxidant compared to the essential oils of lemongrass and thyme in both methods used for the determination of antioxidant activity in vitro (FRAP and DPPH); this makes it a promising source of natural antioxidants that can be used in diets.
当前位置氧化和自由基的问题是对人体健康非常危险的因素之一，这就需要在饮食中寻找安全的天然化合物，并努力对抗自由基。采用FRAP和DPPH两种不同的方法对罗勒、百里香和柠檬草精油的抗氧化作用进行了评价，并采用气相色谱法对各精油的有效成分进行了鉴定。gc - cms分离结果表明，精油的主要成分为罗勒(l -芳樟醇59.02%，1,8-桉叶油脑9.52%)、百里香(百里酚48.06%，对伞花香38.57%)和香茅(香樟47.59%，缬草36.27%)。在FRAP法中，罗勒精油的抗氧化作用最强(2.35±0.61 mMol Fe +2 /g)，其次是百里香精油(1.96±0.42 mMol Fe +2 /g)，其次是柠檬草精油(0.93±0.21 mMol Fe +2 /g)。而DPPH法研究其抗氧化活性时，通过与IC 50值的比较结果表明，罗勒精油的抗氧化能力最强(IC 50 =144.78±7.19 μg/ml)，其次是柠檬草精油(IC 50 =170.05±6.97 μg/ml)，最后是百里香精油(IC 50 =183.45±7.19 μg/ml)，两者差异较小。结果表明，在体外抗氧化活性测定(FRAP和DPPH)两种方法中，罗勒精油的抗氧化作用均优于柠檬草精油和百里香精油;这使得它成为一种很有希望的天然抗氧化剂来源，可以在饮食中使用。
{"title":"A COMPARATIVE STUDY BETWEEN THREE ESSENTIAL OILS IN TERMS OF THEIR CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY","authors":"E. Basiouny, ahmed abdakaway, Mohsen S. Asker, Medhat Mostafa Abozid","doi":"10.21608/mjab.2023.212610.1008","DOIUrl":"https://doi.org/10.21608/mjab.2023.212610.1008","url":null,"abstract":": The problem of oxidation and free radicals is one of the factors that are very dangerous to human health, which requires searching for safe natural compounds to be included in the diet and work to combat free radicals. The aim of this study was to evaluate the antioxidant effect of essential oils of basil, thyme and lemongrass by two different methods (FRAP and DPPH) as well as to identify the active ingredients of each oil by GCMS. The results of the fractionation on the GCMS showed that the main components of the essential oils were as follows: basil (L-linalool 59.02% and 1,8-cineole 9.52%), thyme (Thymol 48.06%and p-cymene 38.57%), and lemongrass (Geranial 47.59% and neral 36.27%). In the FRAP method, essential oil of basil was the strongest as an antioxidant (2.35± 0.61 mMol Fe +2 /g), followed by essential oil of thyme (1.96± 0.42 mMol Fe +2 /g), followed by essential oil of lemongrass by a large margin (0.93± 0.21 mMol Fe +2 /g). Whereas, when studying the antioxidant activity by the DPPH method, the results showed through comparison with IC 50 values that, basil essential oil was the strongest antioxidant (IC 50 =144.78 ± 7.19 μg/ml), followed by lemongrass essential oil (IC 50 =170.05 ± 6.97 μg/ml) and finally thyme essential oil (IC 50 =183.45 ± 7.19 μg/ml), w ith a slight difference. The results clearly indicated that the essential oil of basil was the best as an antioxidant compared to the essential oils of lemongrass and thyme in both methods used for the determination of antioxidant activity in vitro (FRAP and DPPH); this makes it a promising source of natural antioxidants that can be used in diets.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126126518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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