Pub Date : 2023-01-01DOI: 10.21608/mjab.2023.288473
Dina Rasmi Mohammed Elhoshi, R. A. Eissa, A. El- Zanaty
{"title":"Genetic diversity and symbiotic efficiency of Rhizobium leguminosarum nodulating faba bean (Vicia faba)","authors":"Dina Rasmi Mohammed Elhoshi, R. A. Eissa, A. El- Zanaty","doi":"10.21608/mjab.2023.288473","DOIUrl":"https://doi.org/10.21608/mjab.2023.288473","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130928703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/mjab.2022.268991
Y. Ashoush, S. M. El Said
{"title":"Biochemical studies on chitosan","authors":"Y. Ashoush, S. M. El Said","doi":"10.21608/mjab.2022.268991","DOIUrl":"https://doi.org/10.21608/mjab.2022.268991","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134187331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/mjab.2022.268990
Y. Ashoush, A. M. Ali, Medhat Mostafa Abozid, M. Asker
: This study aimed to evaluate the effect of polysaccharide extract of okra, aloe vera and purslane concentration of (100-200 mg / kg of bw) on diabetic rats after injecting them with alloxan concentration of 150 mg/ kg. The chemical composition of okra, aloe vera and Purslane were found that Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, while aloe vera contains 90.22% carbohydrates, 81.74% total soluble polysaccharides, 32% extraction rate, while purslane contains 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage. While the infrared analysis proved in the spectral range the presence of OH, CH 2 , C=O, COO - , S=O, C-O-C, C-O-H and C-O groups, as well as alpha-pyranose rings, and alpha and beta glycosidic bonds for the okra, aloe vera and purslane. An estimated of the molecular weight of the polysaccharides of okra was 5.29 x 10 4 g/mol, aloe vera 6.98 x 10 4 g/mol, and purslane 1.25 x 10 4 g/mol. HPLC analysis of polysaccharides was found which okra contains manuronic acid 0.52, glucose 1.22, mannose 0.96, fucose 0.35 and arabinose 1.0 molar, and aloe vera contains manuronic acid 0.29, glucose 0.35, fructose 1.26, galactose 2.64, and arabinose 1.0 molar, while purslane contains manuronic acid 0.36, glucose 1.51, galactose 1.0 and arabinose 1.6 molar. The study showed that the polysaccharide extract of okra, aloe vera and purslane grown in Egypt was very beneficial in improving the glucose level in the plasma of diabetic rats. And leads to an improvement in the results of glycated hemoglobin. It also improved kidney function (urea - creatinine) and liver functions (GOT - GPT – ALP) and it had a qualitative effect on improving the synthetic functions of the liver (total protein - albumin). Also leads to an improvement in the level of results of the advanced glycated end products. The biological experiment indicated that the results of all groups treated with polysaccharide extract of okra, aloe vera and purslane at a concentration of 100-200 mg/kg improved, and that the best treated groups at all were those treated with polysaccharide extract of okra and aloe vera concentration of 200 mg/kg after 45 days, and this improvement may be due a high molecular weight of okra and aloe vera, or to the presence of some rare monosaccharides such as fucose and mannose in okra, galactose and fructose in aloe vera and high mannuronic acid in plants.
{"title":"Biochemical studies on some polysaccharides and their effects on diabetic rats","authors":"Y. Ashoush, A. M. Ali, Medhat Mostafa Abozid, M. Asker","doi":"10.21608/mjab.2022.268990","DOIUrl":"https://doi.org/10.21608/mjab.2022.268990","url":null,"abstract":": This study aimed to evaluate the effect of polysaccharide extract of okra, aloe vera and purslane concentration of (100-200 mg / kg of bw) on diabetic rats after injecting them with alloxan concentration of 150 mg/ kg. The chemical composition of okra, aloe vera and Purslane were found that Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, while aloe vera contains 90.22% carbohydrates, 81.74% total soluble polysaccharides, 32% extraction rate, while purslane contains 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage. While the infrared analysis proved in the spectral range the presence of OH, CH 2 , C=O, COO - , S=O, C-O-C, C-O-H and C-O groups, as well as alpha-pyranose rings, and alpha and beta glycosidic bonds for the okra, aloe vera and purslane. An estimated of the molecular weight of the polysaccharides of okra was 5.29 x 10 4 g/mol, aloe vera 6.98 x 10 4 g/mol, and purslane 1.25 x 10 4 g/mol. HPLC analysis of polysaccharides was found which okra contains manuronic acid 0.52, glucose 1.22, mannose 0.96, fucose 0.35 and arabinose 1.0 molar, and aloe vera contains manuronic acid 0.29, glucose 0.35, fructose 1.26, galactose 2.64, and arabinose 1.0 molar, while purslane contains manuronic acid 0.36, glucose 1.51, galactose 1.0 and arabinose 1.6 molar. The study showed that the polysaccharide extract of okra, aloe vera and purslane grown in Egypt was very beneficial in improving the glucose level in the plasma of diabetic rats. And leads to an improvement in the results of glycated hemoglobin. It also improved kidney function (urea - creatinine) and liver functions (GOT - GPT – ALP) and it had a qualitative effect on improving the synthetic functions of the liver (total protein - albumin). Also leads to an improvement in the level of results of the advanced glycated end products. The biological experiment indicated that the results of all groups treated with polysaccharide extract of okra, aloe vera and purslane at a concentration of 100-200 mg/kg improved, and that the best treated groups at all were those treated with polysaccharide extract of okra and aloe vera concentration of 200 mg/kg after 45 days, and this improvement may be due a high molecular weight of okra and aloe vera, or to the presence of some rare monosaccharides such as fucose and mannose in okra, galactose and fructose in aloe vera and high mannuronic acid in plants.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125251979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/mjab.2022.268789
A. El- Zanaty, ,. M. Ismaiel, K. Abdel-Lateif
{"title":"SCREENING OF CELLULASE GENES AND DEGRADING ACTIVITIES OF TRICHODERMA ASPERELLUM VIA SOLID-STATE FERMENTATION OF RICE STRAW AND SUGARCANE BAGASSE","authors":"A. El- Zanaty, ,. M. Ismaiel, K. Abdel-Lateif","doi":"10.21608/mjab.2022.268789","DOIUrl":"https://doi.org/10.21608/mjab.2022.268789","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124226264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.21608/mjab.2022.263871
,. A. Ashoush, ,. Ali, ,. M. Abozid, ,. M. Asker, ,. M. Salama
: Such investigation has as its goal to study polysaccharides from okra and purslane. The chemical composition of okra and Purslane is different. Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, against 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage for purslane. The infrared analysis reveald absorption in the region of 1725.18, 1732.38 and 1723.3 cm − 1 for okra and purslane indicated the presence of CH 2 group. The maximal absorption occurs at around 1626.54 cm − 1 of Purslane reflects the polysaccharide property and relates to the stretching vibration of C=O of the N-acetyl group or protonated carboxylic acid . The peaks at 1598.44 and 1408.51 cm − 1 of okra, 1588.32 and 1417.09 cm − 1 of purslane were characteristic to the symmetric stretching of the COO - group, indicating that plants are acidic polysaccharides. The peak located at 1318.56 and 1244.31 cm − 1 of okra, 1376.02 and 1322.88 cm − 1 of purslane correlated with the ester sulphate groups' stretching vibration (S=O) . The ingestion at 1144.54 and 1036.39 cm − 1 of okra, 1024.08 cm − 1 of purslane in the range of 1200 – 1000 cm − 1 which are anomeric regions, were attributed to the existence of C-O bonds in polysaccharides was shown by the compounds C-O-C and C-O- H that the monosaccharides in okra and purslane have α -pyranose ring. The presence of characteristic absorption at 893.03, 821.35 and 780.94 cm − 1 of okra, 826.94, 778.58 and 719.28 cm − 1 of purslane during anomeric area 950 – 700 cm − 1 of plants were assigned to the coexistence of both α and β glycosidic connections. An estimated of the molecular weight of the polysaccharides of okra was 5.29 × 10 4 g/mol and purslane 1.25 × 10 4 g/mol. HPLC analysis of okra polysaccharides was indicated the monosaccharides of using a molar ratio manuronic acid, glucose, mannose, fucose, and arabinose at 0.52, 1.22, 0.96, 0.35, 1.0, respectively. Purslane polysaccharides contained manuronic acid, glucose, galactose and arabinose with molar ratio 0.36, 1.51, 1.0, 1.6, respectively. The 1 H NMR spectra of okra and purslane polysaccharides clearly differs from each other in the pattern of resonances for their glycosyl anomeric and ring protons. The chromatogram of 1 H NMR spectrum exhibited four peaks corresponding to the analysis of linkage and component reported in previous values. Among them, signals at 5.19–5.15 ppm were assigned to α -Arabinose residues, 4.87–4.91 ppm si gnals might represented the residues of α Glucose pyranose.
{"title":"COMPARATIVE STUDY BETWEEN POLYSACCHARIDES ISOLATED FROM OKRA AND PURSLANE","authors":",. A. Ashoush, ,. Ali, ,. M. Abozid, ,. M. Asker, ,. M. Salama","doi":"10.21608/mjab.2022.263871","DOIUrl":"https://doi.org/10.21608/mjab.2022.263871","url":null,"abstract":": Such investigation has as its goal to study polysaccharides from okra and purslane. The chemical composition of okra and Purslane is different. Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, against 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage for purslane. The infrared analysis reveald absorption in the region of 1725.18, 1732.38 and 1723.3 cm − 1 for okra and purslane indicated the presence of CH 2 group. The maximal absorption occurs at around 1626.54 cm − 1 of Purslane reflects the polysaccharide property and relates to the stretching vibration of C=O of the N-acetyl group or protonated carboxylic acid . The peaks at 1598.44 and 1408.51 cm − 1 of okra, 1588.32 and 1417.09 cm − 1 of purslane were characteristic to the symmetric stretching of the COO - group, indicating that plants are acidic polysaccharides. The peak located at 1318.56 and 1244.31 cm − 1 of okra, 1376.02 and 1322.88 cm − 1 of purslane correlated with the ester sulphate groups' stretching vibration (S=O) . The ingestion at 1144.54 and 1036.39 cm − 1 of okra, 1024.08 cm − 1 of purslane in the range of 1200 – 1000 cm − 1 which are anomeric regions, were attributed to the existence of C-O bonds in polysaccharides was shown by the compounds C-O-C and C-O- H that the monosaccharides in okra and purslane have α -pyranose ring. The presence of characteristic absorption at 893.03, 821.35 and 780.94 cm − 1 of okra, 826.94, 778.58 and 719.28 cm − 1 of purslane during anomeric area 950 – 700 cm − 1 of plants were assigned to the coexistence of both α and β glycosidic connections. An estimated of the molecular weight of the polysaccharides of okra was 5.29 × 10 4 g/mol and purslane 1.25 × 10 4 g/mol. HPLC analysis of okra polysaccharides was indicated the monosaccharides of using a molar ratio manuronic acid, glucose, mannose, fucose, and arabinose at 0.52, 1.22, 0.96, 0.35, 1.0, respectively. Purslane polysaccharides contained manuronic acid, glucose, galactose and arabinose with molar ratio 0.36, 1.51, 1.0, 1.6, respectively. The 1 H NMR spectra of okra and purslane polysaccharides clearly differs from each other in the pattern of resonances for their glycosyl anomeric and ring protons. The chromatogram of 1 H NMR spectrum exhibited four peaks corresponding to the analysis of linkage and component reported in previous values. Among them, signals at 5.19–5.15 ppm were assigned to α -Arabinose residues, 4.87–4.91 ppm si gnals might represented the residues of α Glucose pyranose.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130308084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.21608/mjab.2022.263921
,. A. Ashoush, ,. S. El-Sayed, ,. M. Abd-Elwahab
: According to reports, when exposed to hazardous compounds like ethanol, hepatocytes are vulnerable to the harmful effects of oxidants. Chitosan is a potential chemical for the field of toxicity prevention because it possesses a variety of therapeutic qualities. The chemical makeup of chitosan and chitosan nanoparticles were assessed in this study using FTIR spectroscopy, and their particle size and antioxidant activity were assessed in vitro using DPPH. The in vivo hepatoprotective properties against ethanol-induced liver injury were assessed in male Wister rats. In both investigations, silimarin (100 mg/kg b.w.) and chitosan plus chitosan nanoparticles (200 mg/kg b.w.) were given orally. 3.76 gm/kg b.w. of 40% ethanol administered orally for 30 days caused liver damage. In the DPPH scavenging activity assay, chitosan and chitosan nanoparticles both showed antioxidant activity; however, chitosan nanoparticles were more effective than chitosan overall. Hepatotoxicity was evaluated by measuring plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, and plasma antioxidant status (MDA concentration, and catalase activity). Raised plasma AST, ALT, and MDA levels in rats after ethanol treatment showed liver injury. In a preventive model, the administration of chitosan, chitosan nanoparticles, and silimarin reduced the harmful effects of ethanol on the aforementioned plasma parameters. According to the findings of the current investigation, chitosan and chitosan nanoparticles significantly inhibit ethanol-induced liver damage and have antioxidant and hepatoprotective properties.
{"title":"HEPATOPROTECTIVE EFFECTS OF CHITOSAN AND CHITOSAN NANOPARTICLES","authors":",. A. Ashoush, ,. S. El-Sayed, ,. M. Abd-Elwahab","doi":"10.21608/mjab.2022.263921","DOIUrl":"https://doi.org/10.21608/mjab.2022.263921","url":null,"abstract":": According to reports, when exposed to hazardous compounds like ethanol, hepatocytes are vulnerable to the harmful effects of oxidants. Chitosan is a potential chemical for the field of toxicity prevention because it possesses a variety of therapeutic qualities. The chemical makeup of chitosan and chitosan nanoparticles were assessed in this study using FTIR spectroscopy, and their particle size and antioxidant activity were assessed in vitro using DPPH. The in vivo hepatoprotective properties against ethanol-induced liver injury were assessed in male Wister rats. In both investigations, silimarin (100 mg/kg b.w.) and chitosan plus chitosan nanoparticles (200 mg/kg b.w.) were given orally. 3.76 gm/kg b.w. of 40% ethanol administered orally for 30 days caused liver damage. In the DPPH scavenging activity assay, chitosan and chitosan nanoparticles both showed antioxidant activity; however, chitosan nanoparticles were more effective than chitosan overall. Hepatotoxicity was evaluated by measuring plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, and plasma antioxidant status (MDA concentration, and catalase activity). Raised plasma AST, ALT, and MDA levels in rats after ethanol treatment showed liver injury. In a preventive model, the administration of chitosan, chitosan nanoparticles, and silimarin reduced the harmful effects of ethanol on the aforementioned plasma parameters. According to the findings of the current investigation, chitosan and chitosan nanoparticles significantly inhibit ethanol-induced liver damage and have antioxidant and hepatoprotective properties.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129126284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.21608/mjab.2022.230244
,. F. M. Elshouny, ,. S. A. El Kodousy, ,. S. M. Abd Elgawad, ,. M. M. Eldosuky
: The present study was designed to investigate: The chemical composition of artichoke fruit and leaves, the phenolic compounds content, antioxidant activity of fruit and leaves extracts of artichoke , and evaluation the effect of artichoke fruit and leaves on plasma lipid profile. Treatment with artichoke fruit and leaves reduced all lipid profile significantly (triglycerides, total cholesterol, LDL-cholesterol, risk ration and atherogenic index) and that artichoke leaves gave more effect than that of fruit. Reducing power and DPPH assay were used to evaluate the antioxidant activity of aqueous, acetone, methanolic extracts of artichoke fruit and leaves. For reducing power assay the reducing power of methanolic extract of fruit and leaves was higher than the other two extracts. For DPPH assay, the antioxidant potential of artichoke leaves and fruit extracts were further highlighted by the quenching of DPPH free radicals. The values of absorbance for fruit extract ranged from 74.2 to 92.1 and 74.3 to 92 for leaves extracts . The antimicrobial activities against 6 types of bacterial strains, Proteus vulgaris, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Bacillus subtilis, and Streptococcus. The obtained results indicate that 400 mg / ml concentration exhibited the highest inhibitory effect on bacteria activity.
{"title":"LIPID –LOWERING EFFECT OF ARTICKOKE ON PLASMA LIPIDS IN HYPERLIPIDEMIC RATS","authors":",. F. M. Elshouny, ,. S. A. El Kodousy, ,. S. M. Abd Elgawad, ,. M. M. Eldosuky","doi":"10.21608/mjab.2022.230244","DOIUrl":"https://doi.org/10.21608/mjab.2022.230244","url":null,"abstract":": The present study was designed to investigate: The chemical composition of artichoke fruit and leaves, the phenolic compounds content, antioxidant activity of fruit and leaves extracts of artichoke , and evaluation the effect of artichoke fruit and leaves on plasma lipid profile. Treatment with artichoke fruit and leaves reduced all lipid profile significantly (triglycerides, total cholesterol, LDL-cholesterol, risk ration and atherogenic index) and that artichoke leaves gave more effect than that of fruit. Reducing power and DPPH assay were used to evaluate the antioxidant activity of aqueous, acetone, methanolic extracts of artichoke fruit and leaves. For reducing power assay the reducing power of methanolic extract of fruit and leaves was higher than the other two extracts. For DPPH assay, the antioxidant potential of artichoke leaves and fruit extracts were further highlighted by the quenching of DPPH free radicals. The values of absorbance for fruit extract ranged from 74.2 to 92.1 and 74.3 to 92 for leaves extracts . The antimicrobial activities against 6 types of bacterial strains, Proteus vulgaris, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Bacillus subtilis, and Streptococcus. The obtained results indicate that 400 mg / ml concentration exhibited the highest inhibitory effect on bacteria activity.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126028107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.21608/mjab.2022.230246
Dr. F. M. Elshouny, Dr. S. A. ElKodousy, Dr. S. M. Abd El- Gawad
{"title":"Biochemical studies on some medicinal plants.","authors":"Dr. F. M. Elshouny, Dr. S. A. ElKodousy, Dr. S. M. Abd El- Gawad","doi":"10.21608/mjab.2022.230246","DOIUrl":"https://doi.org/10.21608/mjab.2022.230246","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121365328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/mjab.2021.214224
M. Hammam, F. M. Elshony
{"title":"EFFECT OF SOME PLANT EXTRACT AS ANTI-HYPERGLYCEMIC AGENTS","authors":"M. Hammam, F. M. Elshony","doi":"10.21608/mjab.2021.214224","DOIUrl":"https://doi.org/10.21608/mjab.2021.214224","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"332 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123226268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-01DOI: 10.21608/mjab.2021.207622
Omima M. R. Abu Elez, M. Hammam, F. M. Elshony
Background: Hyperglycemia is one of the serious problems that affect humans' lifestyle and require special treatments. Incorrect use of synthetic drugs results in side effects and other problems. Diabetic patients need safer and more effective antidiabetic drugs that decrease diabetic complications. Natural products are harmless due to conventional medicine can be inefficient (e.g. side effects and ineffective therapy). Objective: Evaluate antihyperglycemic activity of aqueous leaves extract of Psidium guajava in hyperglycemic rats. Methods: Adult males albino rats, weighting (140-150 grams) were assigned to one of four groups of six animals each (n= 6). Hyperglycemia induced by single dose intraperitoneal injection of STZ NA (60 mg/kg 110 mg/kg, b.wt i.p.), the control groups was given distilled water, the experimental sub-groups were started one week later of STZ induction, then treated once daily by aqueous extract of Psidium guajava leaves until the end of experiment. Initially, blood samples were taken for measured glucose levels and HbA1c. The body weight, urine sugar and blood glucose levels were checked regularly. Results: The polyphenolic of guava leaves extract was found to be (227.63 mg GAE /g. Ex), while flavonoids content was (79.96 mg QE/g. Ex). However, it caused a significant decrease in blood glucose levels and HbA1c and other glycemic indicators. Conclusion: aqueous extract of Psidium guajava leaves possess significant antidiabetic, and effective in lowering blood glucose level but lack hypoglycemic effect in hyperglycemic rats. Further studies warranted to elucidate possible mechanism(s) of action of the plant material.
{"title":"EFFECT OF AQUEOUS PSIDIUM GUAJAVA LEAVES EXTRACT AS ANTI - HYPERGLYCEMIC AGENT","authors":"Omima M. R. Abu Elez, M. Hammam, F. M. Elshony","doi":"10.21608/mjab.2021.207622","DOIUrl":"https://doi.org/10.21608/mjab.2021.207622","url":null,"abstract":"Background: Hyperglycemia is one of the serious problems that affect humans' lifestyle and require special treatments. Incorrect use of synthetic drugs results in side effects and other problems. Diabetic patients need safer and more effective antidiabetic drugs that decrease diabetic complications. Natural products are harmless due to conventional medicine can be inefficient (e.g. side effects and ineffective therapy). Objective: Evaluate antihyperglycemic activity of aqueous leaves extract of Psidium guajava in hyperglycemic rats. Methods: Adult males albino rats, weighting (140-150 grams) were assigned to one of four groups of six animals each (n= 6). Hyperglycemia induced by single dose intraperitoneal injection of STZ NA (60 mg/kg 110 mg/kg, b.wt i.p.), the control groups was given distilled water, the experimental sub-groups were started one week later of STZ induction, then treated once daily by aqueous extract of Psidium guajava leaves until the end of experiment. Initially, blood samples were taken for measured glucose levels and HbA1c. The body weight, urine sugar and blood glucose levels were checked regularly. Results: The polyphenolic of guava leaves extract was found to be (227.63 mg GAE /g. Ex), while flavonoids content was (79.96 mg QE/g. Ex). However, it caused a significant decrease in blood glucose levels and HbA1c and other glycemic indicators. Conclusion: aqueous extract of Psidium guajava leaves possess significant antidiabetic, and effective in lowering blood glucose level but lack hypoglycemic effect in hyperglycemic rats. Further studies warranted to elucidate possible mechanism(s) of action of the plant material.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133555159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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