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BIOCHEMICAL STUDIES ON THE EFFECT OF ONION ON SOME BLOOD PARAMETERS OF RATS 洋葱对大鼠血液指标影响的生化研究
Pub Date : 2021-11-01 DOI: 10.21608/mjab.2021.207625
M. A. Habib, K. M. Taha, A. Sheta
The objective of the present research was to study chemical composition, phenolic compounds and flavonoids of onion. Onion contained total carbohydrates 70.7%, crude protein 7.35 %, total lipids 1.43%, total ash 2.91% fibers 7.91 % and moisture 9.7% based the dry sample. Total phenols in onion bulbs was 35.23 mg /100g., while the total flavonoids was 23.18 mg/100g. HPLC results showed that onion contained 17 phenolic compounds. There were a high percentage of (benzoic acid 905 mg/kg), caticol (649 mg/kg), risvertol (425 mg/kg), perogalol (157 mg/kg) and naringinin (96 mg/kg). that help to improve some blood parameters such as liver function enzymes, kidneys function, lipids and glucose levels. Onion powder as well as ethanolic and water extracts of onion bulbs were prepared which used in the present treatments. Blood parameters of rats were tested using the methods of A.O.A.C.; (2005). Onion powder used as a treatment with concentration of 2 g% and 4 g% of diet. The ethanolic extract of onion used as a treatment with concentration of 1 g/L and 2 g/L. While water extract used as a treatment with concentration of 1 g/L and 2 g/L for hyperlipidemic rats. Blood parameters of rats were tested after the treatments. Data were subjected to statistical analysis using the SPSS (Software version no. 20). Differences between extracts were tested. The best results were obtained by comparisons of the results shown. Treatments with onion powder and its extracts were beneficial to control the hyperlipidemia, and improved the levels of some blood parameters of rats such as, total cholesterol, HDL-c, LDL-c, TG, ALT, AST, creatinine, urea, uric acid, blood glucose.
本研究的目的是研究洋葱的化学成分、酚类化合物和类黄酮。洋葱总碳水化合物含量为70.7%,粗蛋白质含量为7.35%,总脂质含量为1.43%,总灰分含量为2.91%,纤维含量为7.91%,水分含量为9.7%。洋葱中总酚含量为35.23 mg /100g。总黄酮含量为23.18 mg/100g。HPLC结果显示,洋葱中含有17种酚类化合物。苯甲酸(905毫克/公斤)、钙醇(649毫克/公斤)、芦花醇(425毫克/公斤)、perogalol(157毫克/公斤)和柚皮素(96毫克/公斤)的含量很高。这有助于改善一些血液参数,如肝功能、酶、肾脏功能、血脂和血糖水平。制备了洋葱粉以及洋葱鳞茎的乙醇提取物和水提取物,用于本处理。采用a.o.a.c法测定大鼠血液参数;(2005)。洋葱粉作为处理,浓度分别为日粮的2g和4g。用浓度为1 g/L和2 g/L的洋葱乙醇提取物作为处理。水提取物分别以1 g/L和2 g/L浓度用于高脂血症大鼠。治疗后检测大鼠血液参数。数据采用SPSS(软件版本号:20)。测试了不同提取物之间的差异。通过对所示结果的比较,得到了最佳结果。洋葱粉及其提取物能有效控制高脂血症,改善大鼠总胆固醇、HDL-c、LDL-c、TG、ALT、AST、肌酐、尿素、尿酸、血糖等血液指标。
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引用次数: 0
EFFECT OF SPRAYING BLUE GREEN ALGAE (CYANOBACTERIA) EXTRACTS ON HYBRID RICE SEED PRODUCTION 喷施蓝绿藻(蓝藻)提取物对杂交水稻制种的影响
Pub Date : 2021-11-01 DOI: 10.21608/mjab.2021.207623
H. El-zawawy, E. A. Abd El-aziz, H. Hamad, E. Arafat
Two isolates of cyanobacteria (Anabaena oryzae, Nosto centophytum) were isolated from agricultural wastewater from the experimental farm of Sakha Research Station, Sakha, Kafr EL Sheikh, Egypt for testing their ability to fix atmospheric nitrogen and produce hormones and its effect on hybrid rice seed production by spraying the isolates extract and their mixture on the rice plants. Isolates were varied in their capacity in the extracellular-nitrogen, Intracellular secreted, produce hormones and dry weight of the cyanobacteria isolates (mg/100 ml-culture) where the lowest content was for isolate Anabaena oryzae and the highest was for Nostoc centophytum and mixture. The aim of the present investigation is to identify the effect of cyanobacteria extract isolated from Egyptian waste water on hybrid rice seed production. The used cytoplasmic male sterile lines were IR69625A and G46A and the restorer line was G.178. Application of mixture between Anabaena oryzae and Nostoc centophytum produced the highest values in most studied characters such as seed yield in both seasons.
从埃及Kafr EL Sheikh Sakha研究站实验农场的农业废水中分离出两株蓝藻(Anabaena oryzae, Nosto centophytum),通过将分离物提取物及其混合物喷洒在水稻植株上,测试了它们固定大气氮和产生激素的能力及其对杂交水稻制种的影响。各菌株胞外氮含量、胞内分泌氮含量、产生激素含量和干重含量(mg/100 ml)各不相同,其中稻谷水青菌(Anabaena oryzae)含量最低,心生褐藻(Nostoc centophytum)及其混合物含量最高。本研究的目的是确定从埃及废水中分离的蓝藻提取物对杂交水稻种子生产的影响。使用的细胞质雄性不育系为IR69625A和G46A,恢复系为G.178。在两个季节中,稻稻水草与心叶念珠混合施用对种子产量等大部分性状的影响均最高。
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引用次数: 2
HEPATOPROTECTIVE EFFECTS OF TURMERIC AND MILK THISTLE SEED FLOUR AGAINST ETHANOL LIVER DAMAGE IN WISTER RATS 姜黄和水飞蓟籽粉对大鼠乙醇肝损伤的保护作用
Pub Date : 2021-11-01 DOI: 10.21608/mjab.2021.207624
F. El-Shouny, S. K. El-Kadousy, S. M. El-Gawad, Kh. A. El-Khalwy
Chemical composition of milk thistle seed flour )MTSF) and turmeric (T) were investigated, for milk thistle seed flour, moisture (8.0%), protein (13.0 %), carbohydrates (27.55%), crude lipid (15.0%), ash (10.45%), and crude fiber (26.0%), while turmeric has moisture (8.1%) , protein (9.3 %), carbohydrates (57%), crude lipid (17.0%), ash (6.0%) and crude fiber (2.6%). For total phenol, the highest value in turmeric (11.57mg GAE/g) while milk thistle seed flour (3.39 mg GAE/g)) also total flavonoid for turmeric (4.9mg) while and in milk thistle seed flour (2.34mg). DPPH for milk thistle recorded the highest percent of (FRSA) 98.5 % and turmeric recorded lowest present 82.2% in concentration 100 μg/ml. Reducing power for milk thistle recorded 0.651 but turmeric (T) recorded 0.502 in concentration 100 μg/ml . The identification of phenolic compounds for plant extracts were investigated by HPLC ,the highest content in milk thistle seed flour (MTSF) was Benzoic acid (103.33 ppm) while the lowest content was Caffeine(1.07 ppm) but in turmeric(T) the highest content was Benzoic acid (208.41 ppm), while the lowest content was quercitin (3.08 ppm).The level of plasma aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , alkaline phosphatase (ALP) , total bilirubin , total protein and albumin and plasma antioxidant state SOD , CAT, GPx and MDA were determined to assay hepatotoxicity.
研究了水飞蓟籽粉(MTSF)和姜黄粉(T)的化学成分,其中水飞蓟籽粉的水分(8.0%)、蛋白质(13.0%)、碳水化合物(27.55%)、粗脂肪(15.0%)、灰分(10.45%)和粗纤维(26.0%),姜黄粉的水分(8.1%)、蛋白质(9.3%)、碳水化合物(57%)、粗脂肪(17.0%)、灰分(6.0%)和粗纤维(2.6%)。总酚含量以姜黄最高(11.57mg GAE/g),水飞蓟籽粉最高(3.39 mg GAE/g);总黄酮含量以姜黄最高(4.9mg),水飞蓟籽粉最高(2.34mg)。在浓度为100 μg/ml时,水飞蓟的DPPH最高,为98.5%,姜黄最低,为82.2%。在100 μg/ml浓度下,水飞蓟还原力为0.651,姜黄还原力为0.502。采用高效液相色谱法对植物提取物中的酚类化合物进行了鉴定,发现水飞蓟籽粉(MTSF)中苯甲酸含量最高(103.33 ppm),咖啡因含量最低(1.07 ppm),姜黄(T)中苯甲酸含量最高(208.41 ppm),槲皮素含量最低(3.08 ppm)。测定大鼠血浆天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、总胆红素、总蛋白、白蛋白水平及血浆抗氧化状态SOD、CAT、GPx、MDA,检测大鼠肝毒性。
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引用次数: 0
COMPARATIVE STUDY BETWEEN GINGER, CINNAMON AND CHAMOMILE ESSENTIAL OILS (PHYSICAL PROPERTIES, CHEMICAL PROPERTIES AND CHEMICAL COMPOSITION) 生姜、肉桂和洋甘菊精油的物理性质、化学性质和化学成分比较研究
Pub Date : 2021-08-01 DOI: 10.21608/mjab.2021.193160
H. Mohamed, Y. Ashoush, K. M. Taha, Medhat Mostafa Abozid
Current study designed to compare three important essential oils (Ginger, cinnamon and chamomile) commonly used in Egypt. For this purpose, the essential oils were extracted from ginger rhizomes, cinnamon sticks and chamomile flowers by hydrodistillation; then the physical and chemical properties of these oils were estimated, as well as the identification of the active compounds by using GC-MS. Cinnamon oil recorded the highest values in both specific gravity and refractive index, while ginger oil was the highest in solubility in 80% ethanol. Chamomile oil recorded the highest value in acid number, while the ginger oil was the highest in both saponification number and ester number. GC-MS results identify 12 compounds in ginger oil, 6 compounds in cinnamon oil and 10 compounds in chamomile oil. On the other hand, zingiberene (33.2%) cinnamaldehyde (36.1%) and bisabolol oxide (46.71%) were the main major compounds in ginger oil, cinnamon oil and chamomile oil, respectively. In conclusion the obtained results found that, there are a big difference in physical and chemical properties between these essential oils, they also showed a very different chemical composition which leads to an expected difference in their biological effects.
目前的研究旨在比较埃及常用的三种重要精油(生姜、肉桂和洋甘菊)。为此,采用水蒸气蒸馏法从生姜根茎、肉桂棒和洋甘菊中提取精油;利用GC-MS对其理化性质进行了表征,并对其活性成分进行了鉴定。肉桂油的比重和折射率最高,而姜油在80%乙醇中的溶解度最高。洋甘菊油的酸值最高,姜油的皂化值和酯值均最高。GC-MS分析结果表明,生姜油中含有12种化合物,肉桂油中含有6种化合物,洋甘菊油中含有10种化合物。姜油、肉桂油和洋甘菊油的主要成分分别为姜绿烯(33.2%)、肉桂醛(36.1%)和双abolol氧化物(46.71%)。综上所述,得到的结果发现,这些精油在物理和化学性质上有很大的差异,它们的化学成分也有很大的不同,这导致了它们的生物效应的预期差异。
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引用次数: 0
A COMPARATIVE STUDY BETWEEN GREEN TEA (CAMELLIA SINENSIS) AND COMMON SAGE (SALVIA OFFICINALIS) (CHEMICAL COMPOSITION AND ACTIVE COMPOUNDS) 绿茶(camellia sinensis)与鼠尾草(salvia officinalis)化学成分及活性成分的比较研究
Pub Date : 2021-02-01 DOI: 10.21608/MJAB.2021.169121
S. Draz, A. Ali, K. Hussein, A. Afifi
The purpose of this research is to study the chemical composition and effective compounds of leaves of green tea and sage as one of the traditional medicinal plants. It was found that green tea leaves collected from the local market contain 5.68 % moisture, 2.35% ash, 14.57 % protein, 8.55 % lipids, and 2.3% crude fiber. While sage leaves contained 8.19, 2.17, 12.21, 3.33, and 2.6 % moisture, ash, protein, lipids, and crude fiber, respectively. It was also found that green tea leaves contain 0.376 % Polyphenols and 0.188 % Flavonoids, while sage leaves contain 0.262 and 0.132 % Polyphenols and Flavonoids, respectively. HPLC showed that the aqueous extract of green tea leaves contains eighteen compounds of Polyphenols, fifteen compounds of them were identified. While the aqueous extract of sage leaves contains twenty-one compounds, nineteen of them were identified.
本研究的目的是研究绿茶和鼠尾草叶作为传统药用植物之一的化学成分和有效成分。结果表明,从当地市场采集的绿茶中,水分含量为5.68%,灰分含量为2.35%,蛋白质含量为14.57%,脂肪含量为8.55%,粗纤维含量为2.3%。鼠尾草叶的水分、灰分、蛋白质、脂肪和粗纤维含量分别为8.19%、2.17%、12.21%、3.33%和2.6%。绿茶中多酚类物质含量为0.376%,黄酮类物质含量为0.188%,鼠尾草中多酚类物质含量为0.262,黄酮类物质含量为0.132%。高效液相色谱法测定绿茶水提物中含有18种多酚类化合物,鉴定出15种。鼠尾草叶水提物中含有21种化合物,其中鉴定出19种。
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引用次数: 0
GENETIC ASSESSMENT OF LEAF RUST RESISTANCE IN SOME WHEAT VARIETIES 部分小麦品种抗叶锈病的遗传评价
Pub Date : 2020-12-01 DOI: 10.21608/MJAB.2020.172399
R. A. Eissa, A. Fahmi, A. M. El-Zanaty, W. M. E. Orabey
The experiments of this study were carried out through four growing seasons i.e., 2015/16 to 2018/19 at the farm of the faculty of Agriculture, Shibin El-Kom, Menoufia University.Using10 Egyptian wheat varieties,16 wheat promising lines from (CIMMYT) and 20 adult plant leaf rust resistance genes (Lr). lines Lr were used as (male) for crosses with the wheat cultivars (female) to obtain the F1 grains to produce F2 grains and perform genetic analysis. Recording final rust severity (FRS), rate of disease increase (r-value) and the area under disease progress curve (AUDPC) from varieties and lines to responses leaf rust resistance. While STS marker specific for Lr19 and Lr28 genes were performed on the ten wheat varieties and the sixteen promising lines. The responses of rust severity and infection type of the 20 Lr strains were divided into three groups; I resistant genes i.e., Lr17, Lr18, Lr28, Lr 38 and Lr42, II susceptible genes i.e., Lr2c, Lr9, Lr14b, Lr24, Lr34, Lr35, Lr36, Lr37, Lr44 and Lr45, and III genes with variable reactions i.e. Lr2a, Lr19, Lr29, Lr30 and Lr39. Results suggested that genes of group I are important for breeding for leaf rust resistance under Egyptian field conditions. The ten wheat cultivars exhibited low values of FRS and AUDPC except Sids1, and no variety has high rate of (r-value). These cultivars except Sids1 have a good level of adult plant resistance to leaf rust and can be used as resistance sources, 16 wheat promising lines gave lowest values of (FRS), (r-value) and (AUDPC), and consequently they can be used in breeding programs. Genetic analysis showed that Sids1 did not have any of genes under study. However, Gemmeiza 7, Gemmeiza 9, Gemmeiza 10, Gemmeiza 11, Gemmeiza 12, Misr 1, Misr 2, Giza168 and Giza 171 have several Lr genes. Using STS marker specific for Lr19 and Lr28 genes, Lr19 was identified in Gemmeiza 7 and Gemmeiza 11 and Lr28 detected in Gemmeiza 9 and Gemmeiza 10, while Lr19 detected in 13 promising lines, Lr28 found only in five of them. These results showed that molecular markers for Lr genes might be a useful tool in marker-assisted breeding (MAS) for developing Egyptian wheat cultivars.
本研究在Menoufia大学Shibin El-Kom农业学院的农场进行了四个生长季节(2015/16至2018/19)的实验。利用10个埃及小麦品种、16个CIMMYT小麦潜力品系和20个成体植物抗叶锈病基因(Lr)。以品系Lr作为(雄性)与小麦品种(雌性)杂交,获得F1粒,产生F2粒,并进行遗传分析。记录品种和品系对叶锈病抗性反应的最终锈病严重程度(FRS)、病害增加率(r-value)和病害进展曲线下面积(AUDPC)。同时对10个小麦品种和16个候选品系进行了Lr19和Lr28基因特异性STS标记。将20株Lr菌株的锈病严重程度和感染类型分为3组;抗性基因为Lr17、Lr18、Lr28、lr38和Lr42,易感基因为Lr2c、Lr9、Lr14b、Lr24、Lr34、Lr35、Lr36、Lr37、Lr44和Lr45,易变基因为Lr2a、Lr19、Lr29、Lr30和Lr39。结果表明,在埃及田间条件下,I群基因对抗叶锈病育种具有重要意义。10个小麦品种的FRS和AUDPC值除Sids1外均较低,r值率均不高。除Sids1外,其余品种均具有较好的成株抗叶锈病能力,16个小麦候选品系的(FRS)、(r-value)和(AUDPC)值最低,可作为抗叶锈病资源。遗传分析表明,sis1没有任何正在研究的基因。然而,Gemmeiza 7、Gemmeiza 9、Gemmeiza 10、Gemmeiza 11、Gemmeiza 12、Misr 1、Misr 2、Giza168和Giza 171有多个Lr基因。利用Lr19和Lr28基因特异性STS标记,在黑麦7号和黑麦11号中检测到Lr19基因,在黑麦9号和黑麦10号中检测到Lr28基因,在黑麦9号和黑麦10号中检测到Lr19基因,在黑麦10号和黑麦9号中检测到Lr28基因,在黑麦10号和黑麦9号中检测到Lr28基因。这些结果表明,Lr基因的分子标记可作为标记辅助育种(MAS)的有效工具。
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引用次数: 0
BIOCHEMICAL STUDIES ON MAHOGANY SEEDS 红木种子的生化研究
Pub Date : 2020-11-01 DOI: 10.21608/mjab.2020.127526
M. Hammam, G. Khalil, S. S. A. ElGawad
The present study was designed to investigate the chemical composition of Swietenia mahagoni seeds , identification of phenolic compounds in acetone extract in  mahagoni seeds, studying the fatty acids composition, and evaluation of mahagoni extracts and oil  on the glucose level of diabetes  rats. The chemical composition of seeds were moisture (3.6 %), crude fiber (14 %), ash (3%), crude protein (13 %), crude fat (7.4 %) and total carbohydrate (62.6 %). The fatty acids composition were lignoceric acid (C24) 38.23% followed by stearic acid (C18) 35.69%, meanwhile the unsaturated fatty acid was linoleic acid (C18:2), which accounted for ( 26.08 %). The phenolic compound in acetone extract showed that menthol and nerolidol are the major phenolic ( 12.32 and 10.06 % respectively ). Ttreatment with Swietenia mahagoni seed extracts and oil decreased significantly glucose level , also decreased significantly GOT , GPT , ALP , urea , creatinine and malondialdehyde level   as compared with  hyperglycemic group .
本研究旨在研究马哈古尼种子的化学成分,鉴定马哈古尼种子丙酮提取物中的酚类化合物,研究马哈古尼种子的脂肪酸组成,并评价马哈古尼提取物和油对糖尿病大鼠血糖水平的影响。种子化学成分为水分(3.6%)、粗纤维(14%)、灰分(3%)、粗蛋白质(13%)、粗脂肪(7.4%)和总碳水化合物(62.6%)。脂肪酸组成为木脂酸(C24) 38.23%,硬脂酸(C18) 35.69%,不饱和脂肪酸为亚油酸(C18:2),占26.08%。丙酮提取物中的酚类化合物表明,薄荷醇和橙花醇是主要的酚类化合物,分别占12.32%和10.06%。与高血糖组相比,皂角藤籽提取物和油处理显著降低了葡萄糖水平,显著降低了GOT、GPT、ALP、尿素、肌酐和丙二醛水平。
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引用次数: 0
SWIETENIA MAHAGONI SEEDS ATTENUATE HYPERGLYCEMIA AND PROTECT LIVER IN ALLOXAN-INDUCED DIABETIC RATS 苦参籽对四氧嘧啶诱导的糖尿病大鼠的高血糖及肝脏保护作用
Pub Date : 2020-08-01 DOI: 10.21608/mjab.2020.112874
M. Hammam, G. Kalil, S. Sayed, I. Mohamed
The present study was designed to investigate the chemical composition of Swietenia mahagoni seeds , identification of phenolic compounds in acetone extract in  mahagoni seeds, studying the fatty acids composition, and evaluation of mahagoni extracts and oil  on the glucose level of diabetes  rats. The chemical composition of seeds were moisture (3.6 %), crude fiber (14 %), ash (3%), crude protein (13 %), crude fat (7.4 %) and total carbohydrate (62.6 %). The fatty acids composition were lignoceric acid (C24) 38.23% followed by stearic acid (C18) 35.69%, meanwhile the unsaturated fatty acid was linoleic acid (C18:2), which accounted for ( 26.08 %). The phenolic compound in acetone extract showed that menthol and nerolidol are the major phenolic ( 12.32 and 10.06 % respectively ). Ttreatment with Swietenia mahagoni seed extracts and oil decreased significantly glucose level , also decreased significantly GOT , GPT , ALP , urea , creatinine and malondialdehyde level   as compared with  hyperglycemic group .
本研究旨在研究马哈古尼种子的化学成分,鉴定马哈古尼种子丙酮提取物中的酚类化合物,研究马哈古尼种子的脂肪酸组成,并评价马哈古尼提取物和油对糖尿病大鼠血糖水平的影响。种子化学成分为水分(3.6%)、粗纤维(14%)、灰分(3%)、粗蛋白质(13%)、粗脂肪(7.4%)和总碳水化合物(62.6%)。脂肪酸组成为木脂酸(C24) 38.23%,硬脂酸(C18) 35.69%,不饱和脂肪酸为亚油酸(C18:2),占26.08%。丙酮提取物中的酚类化合物表明,薄荷醇和橙花醇是主要的酚类化合物,分别占12.32%和10.06%。与高血糖组相比,皂角藤籽提取物和油处理显著降低了葡萄糖水平,显著降低了GOT、GPT、ALP、尿素、肌酐和丙二醛水平。
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引用次数: 0
STUDIES ON PROMOTER EXPRESSION OF TOXINS GENES IN SOME FOOD POISONING BACTERIA. 某些食物中毒细菌毒素基因启动子表达的研究。
Pub Date : 2020-05-01 DOI: 10.21608/mjab.2020.110247
W. Mahmoud, A. El-beltagy, S. F. A. Allah
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引用次数: 0
USE OF MICROBIAL LIPOPEPTIDES AGAINST SOME PLANT PATHOGENIC MICROORGANISMS 微生物脂肽对某些植物病原微生物的作用
Pub Date : 2020-05-01 DOI: 10.21608/mjab.2020.110244
W. Mahmoud, A. El-beltagy, S. F. A. Allah
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引用次数: 0
期刊
Menoufia Journal of Agricultural Biotechnology
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