Current Opinion in Chemical Biology最新文献

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Probing ligand-induced local stability shifts: A sensitive approach to identify target proteins and binding sites at the proteomic scale 探测配体诱导的局部稳定性转移：一种在蛋白质组学尺度上识别靶蛋白和结合位点的敏感方法

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-05-31 DOI: 10.1016/j.cbpa.2025.102602

Keyun Wang , Yanan Li , Yanni Ma , Mingliang Ye

Deciphering ligand-induced structural alterations represents a critical frontier in functional proteomics, offering transformative potential for both target identification and mechanistic understanding of ligand actions. Therefore, it is of great interest to develop robust proteomic approaches to uncover the binding relationship between ligands and the targeting proteins. The ligand modification-free approaches have the advantages of bypassing chemical modification and thus are broadly applicable to diverse ligands. Herein, in this review, we will introduce some recent methodology advancements for modification-free approaches in the identification of interactions between ligands and proteins. Especially, we will focus on a newly developed method, peptide-centric local stability assay (PELSA), which was designed to probe the ligand-induced local stability shifts and was demonstrated to have high sensitivity in revealing the binding regions at proteome level. The continued refinement and wider implementation of such technologies are expected to revolutionize fundamental research related with ligand–protein interactions.

破译配体诱导的结构改变是功能蛋白质组学的一个关键前沿，为目标识别和配体作用的机制理解提供了变革潜力。因此，开发强大的蛋白质组学方法来揭示配体与靶蛋白之间的结合关系是非常有意义的。无配体修饰的方法具有绕过化学修饰的优点，因此广泛适用于各种配体。在这篇综述中，我们将介绍一些最新的无修饰方法在鉴定配体和蛋白质之间相互作用方面的进展。特别是，我们将重点关注一种新开发的方法，肽中心局部稳定性测定（PELSA），该方法旨在探测配体诱导的局部稳定性变化，并被证明在蛋白质组水平上揭示结合区域具有很高的灵敏度。这些技术的不断完善和更广泛的实施有望彻底改变与配体-蛋白质相互作用相关的基础研究。

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引用次数: 0

Recent advances in metallophore research uncover functions in quorum sensing, antimicrobial activity, and lanthanide acquisition 最近的研究进展揭示了金属团在群体感应、抗菌活性和镧系元素获取方面的功能

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-19 DOI: 10.1016/j.cbpa.2025.102604

Ghazal Kamyabi, Erica L. Debley, Elizabeth M. Nolan

Metallophores are metal-chelating secondary metabolites that bacteria, fungi, and other organisms produce to acquire essential metal nutrients from their environment. This review highlights some recent discoveries in the field: the role of yersiniabactin in quorum sensing, the capacity of aspergillomarasmine A to inhibit bacterial Ni uptake, and the identification of methylolanthanin, a novel metallophore that enables bacterial lanthanide acquisition.

金属细胞是细菌、真菌和其他生物产生的金属螯合次生代谢物，用于从环境中获取必需的金属营养素。这篇综述重点介绍了该领域的一些最新发现：yersinabactin在群体感应中的作用，曲霉酰胺A抑制细菌Ni摄取的能力，以及甲基孤胺素的鉴定，甲基孤胺素是一种新的金属分子，可以使细菌获得镧系元素。

引用次数: 0

Editorial overview: Transforming intracellular delivery: Innovations in macromolecule transport 编辑概述：转化细胞内递送：大分子运输的创新

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-04-28 DOI: 10.1016/j.cbpa.2025.102599

Jean-Philippe Pellois

引用次数: 0

Ion transport across biological membranes 离子在生物膜上的传输

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-18 DOI: 10.1016/j.cbpa.2025.102606

Robin S. Bon, Matthew J. Langton

引用次数: 0

Recent developments in probing the levels and flux of selected organellar cations as well as organellar mechanosensitivity 在探测选定的细胞器阳离子的水平和通量以及细胞器的机械敏感性方面的最新进展

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-05-03 DOI: 10.1016/j.cbpa.2025.102600

Taufiq Rahman , Sandip Patel

Electrochemical gradients exist not only across the plasma membrane (PM) but also across membranes of organelles. Various endomembrane-localised ion channels and transporters have been identified, the activity of which is critical for organellar (and also cellular) ionic homeostasis that underpins diverse cellular processes. Aberrant organellar ion flux underlies several diseases, identifying organellar channels and transporters as potential drug targets. Therefore, the need for probing the functions of these proteins in situ cannot be overemphasised. The acidic interior of a few organelles as well as the dynamic nature of most organelles historically presented challenges for reliable estimation of luminal ionic concentrations. But there have been significant methodological and technical advancements by now, allowing measurement of levels of specific ions within these organelles as well as their flux across endomembranes with increasing precision. Evidence also continues to amass reporting mechanosensitivity of the endomembranes and its physiological significance. Here we highlight some recent developments in tools and techniques for measuring the levels and movement of some selected organellar cations as well as organellar mechanosensitivity.

电化学梯度不仅存在于质膜上，也存在于细胞器膜上。已经确定了各种膜局部离子通道和转运体，其活性对于支持多种细胞过程的细胞器（和细胞）离子稳态至关重要。异常的细胞器离子通量是几种疾病的基础，确定了细胞器通道和转运体作为潜在的药物靶点。因此，原位探测这些蛋白质功能的必要性再怎么强调也不为过。一些细胞器的酸性内部以及大多数细胞器的动态性质历来为可靠估计腔内离子浓度提出了挑战。但是到目前为止，已经有了重大的方法和技术进步，可以越来越精确地测量这些细胞器内特定离子的水平以及它们穿过内膜的通量。关于膜的机械敏感性及其生理意义的报道也越来越多。在这里，我们重点介绍了一些工具和技术的最新发展，用于测量一些选定的细胞器阳离子的水平和运动以及细胞器的机械敏感性。

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引用次数: 0

Chemical tools to study and modulate glycan-mediated host-bacteria interactions 研究和调节聚糖介导的宿主-细菌相互作用的化学工具

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-04 DOI: 10.1016/j.cbpa.2025.102603

Divya Bhargava , Aniqa Chowdhury , Danielle H. Dube

Glycans cover the surfaces of all cells, where they are poised to mediate a two-way discourse between bacteria and host cells. In some instances, glycan-mediated interactions foster a symbiotic state, and in others, they tip the balance toward disease. Chemical biology approaches have begun to reveal the roles of glycans in host-bacteria interactions and provide novel pathways to modulate these interactions. Here, we highlight recent advances in the development and application of chemical biology tools to delineate the roles of glycans in bacterial adhesion, bacterial evasion of the host immune system, host recognition of bacterial cells, and endogenous mechanisms to maintain symbiosis. Further, we present glycan-based strategies to disrupt host-pathogen interactions and to promote the growth of beneficial bacteria.

聚糖覆盖所有细胞的表面，在那里它们准备介导细菌和宿主细胞之间的双向话语。在某些情况下，聚糖介导的相互作用促进了共生状态，而在其他情况下，它们使平衡向疾病倾斜。化学生物学方法已经开始揭示聚糖在宿主-细菌相互作用中的作用，并提供了调节这些相互作用的新途径。在这里，我们重点介绍了化学生物学工具的发展和应用的最新进展，以描述聚糖在细菌粘附，细菌逃避宿主免疫系统，宿主对细菌细胞的识别以及维持共生的内源性机制中的作用。此外，我们提出了基于聚糖的策略来破坏宿主-病原体相互作用并促进有益细菌的生长。

引用次数: 0

Chemical synthesis and biological functions of carbohydrates 碳水化合物的化学合成及生物功能

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-27 DOI: 10.1016/j.cbpa.2025.102617

You Yang, Biao Yu

引用次数: 0

Editorial: Unusual enzyme reactions in natural products biosynthesis 社论：天然产物生物合成中不寻常的酶反应

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-04-26 DOI: 10.1016/j.cbpa.2025.102597

Taifo Mahmud, Ikuro Abe

引用次数: 0

Creating and understanding new-to-nature chemistry 创造和理解新的自然化学

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-17 DOI: 10.1016/j.cbpa.2025.102621

Sonja Billerbeck, Virginia W. Cornish

引用次数: 0

Recent advances in chemical proteomics for protein profiling and targeted degradation 化学蛋白质组学在蛋白质分析和靶向降解方面的最新进展

IF 6.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Opinion in Chemical Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-26 DOI: 10.1016/j.cbpa.2025.102605

Peng-Kai Liu , Zicong Wang , Lingjun Li

Chemical proteomics has emerged as a powerful approach to decipher protein function, interactions, and targeted degradation pathways in complex biological systems. Recent advances in chemical labeling strategies, including activity-based protein profiling (ABPP), proximity labeling (PL), and proteolysis-targeting chimeras (PROTACs), have facilitated a deeper understanding of protein function and interaction networks. First, ABPP employs covalent probes to selectively label active enzymes, uncovering functional proteomics and drug–target interactions. Innovations such as PhosID-ABPP and streamlined cysteine ABPP have improved site-specific quantification and throughput, enabling proteome-wide analysis of enzyme activity and small-molecule interactions. Second, PL enables the characterization of transient protein–protein interactions using enzymatic or chemically triggered approaches. Advances including TurboID and TransitID enhanced the spatiotemporal resolution of PL. Third, PROTACs expand the scope of targeted protein degradation by leveraging the ubiquitin–proteasome system. Collectively, we highlight recent advancements in integrating mass spectrometry (MS) with these methodologies in the field of chemical proteomics.

化学蛋白质组学已经成为一种破译复杂生物系统中蛋白质功能、相互作用和靶向降解途径的有力方法。化学标记策略的最新进展，包括基于活性的蛋白质分析（ABPP）、接近标记（PL）和蛋白水解靶向嵌合体（PROTACs），促进了对蛋白质功能和相互作用网络的深入了解。首先，ABPP采用共价探针选择性标记活性酶，揭示功能蛋白质组学和药物-靶标相互作用。PhosID-ABPP和流线型半胱氨酸ABPP等创新技术提高了位点特异性定量和通量，使酶活性和小分子相互作用的蛋白质组范围分析成为可能。其次，PL能够利用酶或化学触发的方法表征瞬时蛋白质-蛋白质相互作用。TurboID和TransitID等进展增强了PL的时空分辨率。第三，PROTACs利用泛素-蛋白酶体系统扩大了靶向蛋白降解的范围。总的来说，我们强调了在化学蛋白质组学领域将质谱（MS）与这些方法相结合的最新进展。

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