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Assessment of Wood Chips and Agricultural Residues as Denitrifying Bioreactor Feedstocks for Use in the Canadian Prairies 木屑和农业残留物作为加拿大草原反硝化生物反应器原料的评价
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15412
J. Kohn, Gregory S. Piorkowski, Nicole E. Seitz Vermeer, Janelle F. Villeneuve
Highlights Performance of denitrifying bioreactors in Alberta was evaluated. Barley straw was more effective in reducing nitrate compared to wood chips. Hydraulic retention time, feedstock, and season are the primary factors affecting nitrate removal. Abstract. This study evaluated the performance of pilot-scale denitrifying bioreactors (LWD: 6 × 0.6 × 1m) filled with different carbon substrates, including barley straw, hemp straw, and woodchips, for removing dissolved nitrogen from simulated subsurface drainage at two representative geographic locations in Alberta. In this study, the bioreactors were tested under varying hydraulic retention times (4, 8, and 12 h) in the spring, summer, and fall of one year. Tracer studies were conducted to evaluate flow and dispersion characteristics. The mean of nitrate removal efficiency ranged from 19% to 87% during the spring, 44% to 95% during the summer, and 21% to 68% during the fall. We found that barley straw was more effective in reducing nitrate (45% to 95%) compared to wood chips (19% to 54%). This study is the first testing of the effect of different biomass types and hydraulic residence times on bioreactor performance in the Canadian prairies (Alberta) and will allow agricultural producers and regulators to assess the suitability of these systems within the region. Keywords: Bioreactor, Denitrification, Water quality, Wood chips, Agricultural residues, Subsurface Drainage.
对艾伯塔省反硝化生物反应器的性能进行了评价。与木屑相比,大麦秸秆对硝酸盐的还原效果更好。水力停留时间、原料和季节是影响硝酸盐去除的主要因素。摘要本研究在阿尔伯塔省两个具有代表性的地理位置,对不同碳基质(包括大麦秸秆、大麻秸秆和木屑)填充的中试反硝化生物反应器(LWD: 6 × 0.6 × 1m)去除模拟地下排水中的溶解氮的性能进行了评估。在本研究中,生物反应器在一年的春季、夏季和秋季分别在不同的水力滞留时间(4、8和12小时)下进行了测试。进行了示踪剂研究,以评估流动和分散特性。硝态氮的平均去除率春季为19% ~ 87%,夏季为44% ~ 95%,秋季为21% ~ 68%。我们发现大麦秸秆在减少硝酸盐(45%至95%)方面比木屑(19%至54%)更有效。这项研究是在加拿大大草原(阿尔伯塔)首次测试不同生物量类型和水力停留时间对生物反应器性能的影响,并将使农业生产者和监管机构能够评估这些系统在该地区的适用性。关键词:生物反应器,反硝化,水质,木屑,农业废弃物,地下排水
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引用次数: 0
Denitrifying Bioreactor In Situ Woodchip Bulk Density 反硝化生物反应器原位木屑堆积密度
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15364
L. Christianson, R. Christianson, C. Díaz-García, G. Johnson, B. Maxwell, R. Cooke, N. Wickramarathne, L. Gentry
Highlights The bulk density of woodchips in denitrifying bioreactors in the field is unknown. In situ bulk density estimation methods were developed for use during construction or excavation. Dry bulk densities of aged woodchips at bioreactor bottoms were lower than previous literature values. Moisture and particle size and density explained some, but not all, of the variation in in situ bulk densities. Abstract. Woodchip bulk density in a denitrifying bioreactor governs system hydraulics, but this prime physical attribute has never been estimated in situ. The objectives were twofold: (1) to establish estimates of in situ woodchip bulk density at bioreactors in the field, and (2) evaluate causal factors for and resulting impacts of these estimates. Proof-of-concept bulk density methods were developed at a pilot-scale bioreactor using three ways to estimate volume: surveying the excavated area, pumping the excavation full through a flow meter, and using iPhone Light Detection and Ranging (LiDAR). These methods were then further tested at two new and three old full-size bioreactors. Additional ex situ (off-site) testing with the associated woodchips included analysis of bulk density along a moisture gradient and particle size, particle density, wood composition, and hydraulic property testing. In situ dry bulk densities based on the entire volume of the new bioreactors (206-224 kg/m3) were similar to values from previous lab-scale studies. In situ estimates for woodchips at the bottom of aged bioreactors (22-mo. to 6-y) were unexpectedly low (120-166 kg/m3), given that these woodchips would presumably be the most compacted. These low moisture-content corrected dry bulk densities were influenced by high moisture contents in situ (>70% wet basis). The impacts of particle size and particle density on bulk density were somewhat mixed across the dataset, but in general, smaller woodchips had higher dry bulk densities than larger, and several woodchips sourced from the bottom of bioreactors had low particle densities. Although dry bulk densities in the zone of flow in bioreactors in the field were shown to be relatively low, the resulting permeability coefficients under those packing conditions did not differ from those of the original woodchips. The LiDAR-based volume estimation method was the most practical for large-scale, full-size evaluations and allowed high precision with small features (e.g., vertical reactor edges, drainage fittings). Keywords: Compaction, Cone penetrometer, Drainable porosity, LiDAR, Moisture content, Survey.
该领域反硝化生物反应器中木屑的堆积密度尚不清楚。就地容重估算方法是为施工或开挖时使用而开发的。老化木屑在生物反应器底部的干容重低于以往的文献值。水分和颗粒大小和密度解释了一些,但不是全部,在原地堆积密度的变化。摘要反硝化生物反应器中木屑的体积密度决定了系统水力学性能,但这一主要物理属性从未在现场进行过估计。目的有两个:(1)建立实地生物反应器中原位木屑堆积密度的估计值,(2)评估这些估计值的因果因素和由此产生的影响。概念验证的体积密度方法是在一个中试规模的生物反应器中开发的,使用三种方法来估计体积:测量挖掘面积,通过流量计泵满挖掘,以及使用iPhone光探测和测距(LiDAR)。这些方法随后在两个新的和三个旧的全尺寸生物反应器上进行了进一步的测试。额外的非原位(非现场)木屑测试包括沿湿度梯度和颗粒大小、颗粒密度、木材成分和水力性能测试的体积密度分析。基于新生物反应器整体体积的原位干容重(206-224 kg/m3)与先前实验室规模研究的值相似。对老化生物反应器底部木屑的原位估计(22个月)。考虑到这些木片可能是最致密的,到6-y)出乎意料地低(120-166 kg/m3)。这些低含水量修正后的干容重受到原位高含水量(>70%湿基)的影响。颗粒大小和颗粒密度对堆积密度的影响在整个数据集中有些混合,但一般来说,较小的木片比较大的木片具有更高的干堆积密度,并且来自生物反应器底部的一些木片具有低的颗粒密度。尽管在野外生物反应器中流动区的干容重相对较低,但在这些包装条件下产生的渗透系数与原始木片的渗透系数没有差异。基于激光雷达的体积估计方法对于大规模、全尺寸的评估是最实用的,并且可以在小特征(例如垂直反应器边缘、排水接头)上实现高精度。关键词:压实,锥贯仪,可排水孔隙度,激光雷达,含水率,测量
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引用次数: 0
Design of Non-Thermal Plasma Alfalfa Seed Vigor Enhancement Device and Study of Treatment Effect 非热等离子体苜蓿种子活力增强装置设计及处理效果研究
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15309
Yunting Hui, Yangyang Liao, Sibiao Li, Changyong Shao, Decheng Wang, Yong You
Highlights An effective seed treatment method is provided. Three generations of field growth trials were conducted. We Investigated the effects of low-temperature plasma treatment on the biological characters and yield components. Abstract. An atmospheric pressure, low-temperature dielectric barrier discharge (DBD) plasma seed treatment device was developed for plasma seed treatment. The device worked continuously on alfalfa seeds and evenly distributed the seeds in a plasma discharge range. The processing time, voltage amplitude, and frequency were adjustable. The device was used to study the effect of DBD plasma treatment at different voltages and times on alfalfa seed germination using untreated alfalfa seeds as the control (CK). The results showed that the DBD plasma treatment of alfalfa seeds promoted seed germination and seedling growth, and the optimal discharge conditions were a discharge voltage of 11 kV and a discharge time of 40 s. Compared with CK, the germination potential and germination rate increased by 12.49% and 18.08%, respectively. After treatment using the optimal discharge time, the germination potential, germination rate, dry weight, and seedling height increased by 9.9%, 16.1%, 15%, and 32.9%, respectively, compared with CK. The Scanning Electron Microscope images of the seed epidermis treated with 11 kV and 40 s plasma showed that the surface of alfalfa seeds was etched. Different doses of discharge radiation had different effects on physiological processes in seeds, and their sensitivity to plasma discharge was different. In a certain range, the germination rate, germination potential, fresh weight, dry weight, root length, and seedling height of alfalfa seeds improved to different degrees under different discharge voltages and times. Plasma has a good application prospect for improving the growth of alfalfa seeds. Keywords: Alfalfa, Dielectric barrier discharge plasma, Germination, Seed treatment device, Seedling growth.
提供了一种有效的种子处理方法。进行了三代田间生长试验。研究了低温等离子体处理对玉米生物学特性和产量组成的影响。摘要研制了常压低温介质阻挡放电(DBD)等离子体种子处理装置。该装置对紫花苜蓿种子连续工作,并在等离子体放电范围内均匀分布。处理时间、电压幅值和频率可调。利用该装置,以未处理的苜蓿种子(CK)为对照,研究了不同电压、不同时间的DBD等离子体处理对苜蓿种子萌发的影响。结果表明:DBD等离子体处理苜蓿种子能促进种子萌发和幼苗生长,最佳放电条件为放电电压为11 kV,放电时间为40 s;与对照相比,萌发势和发芽率分别提高了12.49%和18.08%。采用最佳排水时间处理后,萌发势、发芽率、干重和苗高分别比对照提高了9.9%、16.1%、15%和32.9%。经11kv和40s等离子体处理后的苜蓿种子表皮扫描电镜显示,种子表面有蚀刻现象。不同剂量的放电辐射对种子生理过程的影响不同,对等离子体放电的敏感性也不同。在一定范围内,不同放电电压和次数对苜蓿种子的发芽率、萌发势、鲜重、干重、根长、苗高均有不同程度的提高。等离子体在促进苜蓿种子生长方面具有良好的应用前景。关键词:苜蓿,介质阻挡放电等离子体,萌发,种子处理装置,幼苗生长
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引用次数: 0
Perspective: Preparing Leaders to Engineer Sustainability and Resilience Across the Food Chain Through the Grand Challenges Scholars Program 视角:通过“大挑战学者计划”培养领导者在整个食物链中设计可持续性和弹性
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.14915
Richard K. Miller, Y. Yortsos
Highlights Addressing the complex political, economic, and societal challenges inherent in sustainable agriculture and food production requires interdisciplinary thinking and approaches. Relevant pedagogical models and extracurricular experiences can be provided by the Grand Challenges Scholars Program, now spread to nearly 100 universities globally. The complexities of agriculture and food production today can be addressed by future engineering leaders based on this program. Abstract. The education of engineers and other professionals to address the global grand challenge of sustainable food production will require much more than excellent technical skills. New mindsets, human-centered design principles, and collaborative leadership skills will be required to develop leaders who will be successful in addressing the complex political, economic, and societal challenges inherent in sustainable agriculture and food production today. This will require supplementing—not replacing—the technical core of engineering education with new pedagogical models and extracurricular experiences. One such model that has proven effective in this area and has spread to nearly 100 universities globally is the Grand Challenges Scholars Program. This article explains how the complexities of agriculture and food production today can be addressed by future engineering leaders based on this program. Keywords: Food chain, Sustainable agriculture.
解决可持续农业和粮食生产中固有的复杂的政治、经济和社会挑战需要跨学科的思维和方法。相关的教学模式和课外经验可以通过“大挑战学者计划”提供,该计划目前已推广到全球近100所大学。今天的农业和粮食生产的复杂性可以解决未来的工程领导者基于这一计划。摘要工程师和其他专业人员的教育,以解决可持续粮食生产的全球巨大挑战,将需要的远远超过优秀的技术技能。新的思维方式,以人为本的设计原则和协作领导技能将需要培养领导者,他们将成功地解决当今可持续农业和粮食生产中固有的复杂政治,经济和社会挑战。这需要用新的教学模式和课外经验来补充——而不是取代——工程教育的技术核心。其中一个已被证明在这一领域行之有效的模式是“大挑战学者计划”(Grand Challenges Scholars Program),它已推广到全球近100所大学。这篇文章解释了当今农业和粮食生产的复杂性是如何由未来的工程领导者基于这个项目来解决的。关键词:食物链;可持续农业;
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引用次数: 0
Air-Pinch PWM Valve to Regulate Flow Rate of Hollow-Cone Nozzles for Variable-Rate Sprayers 气夹式PWM阀调节变流量喷雾器空心锥喷嘴流量
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15601
Javier Campos, Heping Zhu, Hongyoung Jeon, Ramón Salcedo, Erdal Ozkan, Carla Roman, Emilio Gil
Highlights Air-pinch PWM valve was investigated as an alternative to electric PWM valves to manipulate hollow-cone nozzles. Air-pinch and electric PWM valves performed comparable accuracy in flow rate modulations. Droplet sizes from hollow-cone nozzles with both PWM valves were comparable across DUCs ranging from 20% to 100%. Air-pinch PWM valve had great potential of use due to its capacity to isolate the internal parts of the valve from chemicals. Abstract. Electric pulse width modulation (PWM) solenoid valves are commonly used to regulate nozzle flow rates to achieve precision variable-rate spray applications. However, some pesticide formulations, such as wettable powders and adhesive additives, can potentially cause a malfunction such that the valve cannot completely shut off during flow rate modulation if spray lines are not cleaned thoroughly after spray applications. An air-pinch PWM valve was evaluated as a potential alternative to conventional PWM valves to modulate the flow rates of hollow-cone nozzles used on air-assisted orchard sprayers. With the air-pinch valve, spray mixtures only passed through a flexible tube to avoid chemicals directly contacting the moving components inside the valve chamber. The flow rate modulation was performed by pinching and releasing the tube back and forth with air-pilot PWM actions. Evaluations included the flow rate modulation capability along with droplet size distributions from three disc-core hollow-cone nozzles coupled with the PWM pinch valve and compared with a conventional electric PWM valve. Both air-pinch and electric PWM valves performed comparably in the flow rate modulation accuracy and droplet size distribution for hollow-cone nozzles operated at 414 and 827 kPa pressures across the duty cycles (DUCs) ranging from 10% to 100%, except for the air-pinch valve that could not activate at 10% DUC. The flow rates of nozzles modulated with both PWM valves at all DUCs were 5.3% greater on average than the target flow rates, while the flow rates were similar at 90% and 100% DUCs. Droplet size classifications based on ASABE Standard S-572.3 were generally consistent across DUCs ranging from 20% to 100% for the same nozzle and pressure with the air-pinch PWM valve and from 10% to 100% with the conventional electric PWM valve. The consistency of droplet sizes across DUCs and accuracy of flow rate modulations demonstrated the potential advantage of using the air-pinch PWM solenoid valve as an alternative for precision variable-rate sprayers to accurately apply different chemicals. Keywords: Droplet size, Flow rate control, Pesticide, Pinch valve, Precision farming, Pulse width modulation.
研究了气夹式脉宽调制阀作为电动脉宽调制阀的替代方案来控制空心锥喷嘴。气夹和电动PWM阀在流量调节方面具有相当的精度。两种PWM阀的空心锥喷嘴的液滴尺寸在DUCs范围从20%到100%之间具有可比性。气夹式PWM阀具有很大的使用潜力,因为它能够将阀的内部部件与化学品隔离开来。摘要电脉冲宽度调制(PWM)电磁阀通常用于调节喷嘴流量,以实现精确的可变速率喷雾应用。然而,一些农药配方,如可湿性粉末和粘合剂添加剂,可能会导致潜在的故障,如在喷雾剂应用后,如果喷雾剂管线没有彻底清洁,则在流量调节期间阀门不能完全关闭。对气夹式脉宽调制阀作为传统脉宽调制阀的潜在替代方案进行了评估,以调节空气辅助果园喷雾器上使用的空心锥喷嘴的流量。使用气夹阀,喷雾混合物仅通过柔性管,避免化学物质直接接触阀腔内的运动部件。流量调制是通过气动PWM动作前后捏放管来实现的。评估包括流量调节能力以及三个圆盘芯空心锥喷嘴与PWM夹管阀的液滴大小分布,并与传统的电动PWM阀进行比较。除了气夹阀在10%到100%的占空比(DUC)范围内无法启动外,空锥喷嘴在414和827 kPa压力下的流量调节精度和液滴尺寸分布方面表现相当。使用两种PWM阀调制的喷嘴在所有DUCs时的流量平均比目标流量大5.3%,而在90%和100% DUCs时的流量相似。基于ASABE标准S-572.3的液滴尺寸分类在相同喷嘴和压力下,空气夹点式PWM阀的DUCs范围从20%到100%基本一致,而传统电动PWM阀的DUCs范围从10%到100%。DUCs液滴大小的一致性和流量调节的准确性表明,使用气夹式PWM电磁阀作为精密可变速率喷雾器的替代方案,可以准确地喷洒不同的化学品,具有潜在的优势。关键词:液滴大小,流量控制,农药,捏阀,精准农业,脉宽调制
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引用次数: 0
Static and Dynamic Performance Evaluation of a Solid-State LiDAR for 3D Object Detection in Greenhouse Spray Applications 用于温室喷雾剂三维目标检测的固态激光雷达的静态和动态性能评估
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15285
Zhihong Zhang, Jianing Long, Qinghui Lai, Qingmeng Zhu, Hao He, Ramón Salcedo, Tingting Yan
Highlights Comprehensive evaluation of the measurement accuracy of an inexpensive solid-state LiDAR for object detection. Development of an algorithm to acquire point clouds of objects with various shapes under both static and dynamic conditions. Utilization of pseudo-color images to assess the surfaces of regular-shaped cartons and irregular artificial plants. Proposal for integrating the solid-state LiDAR into variable-rate spray applications for greenhouses. Abstract. An effective variable-rate spraying system for greenhouses requires accurate canopy structure parameters of plants to ensure proper pesticide dosage adjustment. While conventional laser systems integrated into spray systems can provide precise point cloud data of plants, they still present a high expense. This study examines the performance of a recently introduced, cost-effective, and high-resolution solid-state LiDAR (Intel RealSense L515) in relation to its potential for greenhouse spray applications. Additionally, a specialized point cloud acquisition algorithm was developed for this solid-state LiDAR to obtain the geometrical parameters of objects. To assess the LiDAR sensor's suitability for greenhouse spray applications, the performance of the LiDAR sensor and the algorithm was evaluated using five different sized regular-shaped cartons and three artificial plants with complex geometry. Various factors were analyzed, such as the horizontal distances between objects and the LiDAR sensor (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 m), the tilt angle of the LiDAR sensor relative to the ground (45°, 60°, and 75°), the height of the LiDAR sensor from the ground (ranging from 0.3 to 0.8 m with 0.5 m distance intervals), and the forward speed of the LiDAR sensor (0.1, 0.3, 0.6, and 0.9 m s-1). The findings revealed that the optimal detection distance for this LiDAR sensor is 1.0 m. Increasing or decreasing the detection distance of the object relative to the LiDAR sensor diminished the measurement accuracy. The accuracy of the derived geometrical variables was affected by the height and tilt angle of the LiDAR sensor. Nevertheless, the geometrical parameters obtained from the solid-state LiDAR showed a favorable correspondence with the results of manual measurements. The highest root mean square error (RMSE) and coefficient of variation (CV) for the overall test were 14.3 mm and 14.3% in the X (length) direction, 14.3 mm and 14.3% in the Y (width) direction, and 10.8 mm and 10.8% in the Z (height) direction, respectively. The contour Edge Similarity Score for objects measured using the solid-state LiDAR and images obtained with an RGB camera exceeded 0.90. These findings suggest that the proposed solid-state LiDAR and the specifically designed algorithm could be effectively adapted to acquire the geometrical parameters of objects and to develop precise variable-rate spraying systems for greenhouse applications. Keywords: Canopy structure measurements, Point cloud, Precision agriculture, Pr
重点介绍了用于目标检测的廉价固态激光雷达测量精度的综合评估。开发了一种静态和动态条件下获取不同形状物体点云的算法。利用伪彩色图像评估规则形状的纸盒和不规则的人造植物的表面。将固态激光雷达集成到温室可变速率喷雾应用中的建议。摘要一个有效的温室变喷系统需要准确的植物冠层结构参数,以保证适当的农药用量调整。传统的激光系统集成到喷雾系统中可以提供精确的植物点云数据,但仍然存在较高的费用。本研究考察了最近推出的具有成本效益的高分辨率固态激光雷达(英特尔RealSense L515)的性能及其在温室喷雾应用中的潜力。此外,还针对该固态激光雷达开发了一种专门的点云获取算法,以获取目标的几何参数。为了评估激光雷达传感器在温室喷雾应用中的适用性,使用五个不同大小的规则形状的纸箱和三个具有复杂几何形状的人工植物来评估激光雷达传感器和算法的性能。分析了物体与激光雷达传感器之间的水平距离(0.5、1.0、1.5、2.0、2.5和3.0 m)、激光雷达传感器相对于地面的倾斜角(45°、60°和75°)、激光雷达传感器距离地面的高度(0.3 ~ 0.8 m,间隔0.5 m)以及激光雷达传感器的前进速度(0.1、0.3、0.6和0.9 m s-1)等因素。结果表明,该激光雷达传感器的最佳探测距离为1.0 m。增加或减少物体相对于激光雷达传感器的探测距离会降低测量精度。激光雷达传感器的高度和倾斜角度会影响几何变量的精度。然而,从固态激光雷达获得的几何参数显示出与人工测量结果良好的对应关系。总体检验的均方根误差(RMSE)和变异系数(CV)在X(长度)方向上分别为14.3 mm和14.3%,在Y(宽度)方向上分别为14.3 mm和14.3%,在Z(高度)方向上分别为10.8 mm和10.8%。使用固态激光雷达测量的物体和使用RGB相机获得的图像的轮廓边缘相似度得分超过0.90。这些发现表明,所提出的固态激光雷达和专门设计的算法可以有效地用于获取物体的几何参数,并开发用于温室应用的精确可变速率喷涂系统。关键词:冠层结构测量,点云,精准农业,精准喷雾技术,可变速率喷雾系统
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引用次数: 0
Inorganic Carbon-Limited Freshwater Algal Growth at High Ph: Revisited with Focus on Alkalinity 无机碳限制淡水藻类在高Ph下的生长:重访碱度
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15411
Mary Katherine Watson, Elizabeth Flanagan, Caye M. Drapcho
Highlights Non-carbonate components of BG11 media impact TIC calculation on average 4.00 mg/L at high pH. BG11 media non-carbonate alkalinity (NCA) varies with pH: NCA (meq/L) = 0.0393×e 0.2075×pH + (2.086×10 -9 )e 1.860×pH . Monod kinetic constants with CO 2 , HCO 3 - , and CO 3 2- as inorganic carbon sources are improved from a previous report. Kinetic constants continue to be the only known reports considering multiple inorganic carbon sources. Algal stoichiometric reactions are developed that account for variation in cell content and carbon source. Abstract. Due to increasing atmospheric CO2, algal growth systems at high pH are of interest to support enhanced diffusion and carbon capture. Given the interactions between algal growth, pH, and alkalinity, data from Watson and Drapcho (2016) were re-examined to determine the impact of the non-carbonate constituents in BG11 media on estimates of Monod kinetic parameters, biomass yield, and cell stoichiometry. Based on a computational method, non-carbonate alkalinity (NCA) in BG11 media varies with pH according to: NCA (meq/L) = 0.0393×e0.2075×pH + (2.086×10-9)e1.860×pH (R2 = 0.999) over the pH range of 10.3 – 11.5. Updated maximum specific growth rates were determined to be 0.060, 0.057, and 0.051 hr-1 for CO2, HCO3, and CO3, respectively. Generalizable stoichiometric algal growth equations that consider variable nutrient ratios and multiple inorganic carbon species were developed. Improved kinetic and stoichiometric parameters will serve as the foundation for a dynamic mathematical model to support the design of high pH algal carbon capture systems. Keywords: Algae, Alkalinity, Carbon Abatement, Carbon Capture, Kinetics, Stoichiometry, Total Inorganic Carbon.
BG11介质的非碳酸盐组分影响TIC计算,在高pH下平均为4.00 mg/L。BG11介质的非碳酸盐碱度(NCA)随pH值的变化而变化:NCA (meq/L) = 0.0393×e 0.2075×pH + (2.086×10 -9)e 1.860×pH。以co2、hco3 -和co32 -为无机碳源时,Monod动力学常数比以往的报道有所提高。动力学常数仍然是唯一已知的考虑多种无机碳源的报告。藻类化学计量反应的发展,说明变化的细胞含量和碳源。摘要由于大气中二氧化碳的增加,高pH值下的藻类生长系统支持增强扩散和碳捕获。考虑到藻类生长、pH值和碱度之间的相互作用,我们重新检查了Watson和Drapcho(2016)的数据,以确定BG11培养基中非碳酸盐成分对Monod动力学参数、生物量产量和细胞化学计量学的影响。根据计算方法,BG11培养基的非碳酸盐碱度(NCA)随pH值的变化规律为:在10.3 ~ 11.5的pH范围内,NCA (meq/L) = 0.0393×e0.2075×pH + (2.086×10-9)e1.860×pH (R2 = 0.999)。CO2、HCO3和CO3的更新最大比生长率分别为0.060、0.057和0.051 hr-1。建立了考虑可变营养比和多种无机碳种的藻类生长广义化学计量方程。改进的动力学和化学计量参数将作为动态数学模型的基础,以支持高pH藻类碳捕获系统的设计。关键词:藻类,碱度,碳减排,碳捕获,动力学,化学计量学,总无机碳
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引用次数: 0
A Multimodal Optical Sensing System for Automated and Intelligent Food Safety Inspection 用于食品安全自动化智能检测的多模态光学传感系统
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15526
J. Qin, Jeehwa Hong, Hyunjeong Cho, J. V. Van Kessel, I. Baek, K. Chao, M. Kim
Highlights A multimodal optical sensing system was developed for food safety applications. The prototype system can conduct dual-band Raman spectroscopy at 785 and 1064 nm. The system can automatically measure samples in Petri dishes or well plates. The system with AI software is promising for identifying species of foodborne bacteria. Abstract. A novel multimodal optical sensing system was developed for automated and intelligent food safety inspection. The system uses two pairs of compact point lasers and dispersive spectrometers at 785 and 1064 nm to realize dual-band Raman spectroscopy and imaging, which is suitable to measure samples generating low- and high-fluorescence interference signals, respectively. Automated spectral acquisition can be performed using a direct-drive XY moving stage for solid, powder, and liquid samples placed in customized well plates or randomly scattered in standard Petri dishes (e.g., bacterial colonies). Three LED lights (white backlight, UV ring light, and white ring light) and two miniature color cameras are used for machine vision measurements of samples in the Petri dishes using different combinations of illuminations and imaging modalities (e.g., transmission, fluorescence, and color). Real-time image processing and motion control techniques are used to implement automated sample counting, positioning, sampling, and synchronization functions. System software was developed using LabVIEW with integrated artificial intelligence functions able to identify and label interesting targets instantly. The system capability was demonstrated by an example application for rapid identification of five common foodborne bacteria, including Bacillus cereus, E. coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp.. Using a machine learning model based on a linear support vector machine, a classification accuracy of 98.6% was achieved using Raman spectra automatically collected from 222 bacterial colonies of the five species grown on nutrient nonselective agar in 90 mm Petri dishes. The entire system was built on a 30×45 cm2 breadboard, enabling it compact and portable and its use for field and on-site biological and chemical food safety inspection in regulatory and industrial applications. Keywords: Artificial intelligence, Automated sampling, Bacteria, Food safety, Machine learning, Machine vision, Raman, Sensing.
开发了一种用于食品安全的多模态光学传感系统。原型系统可以在785和1064 nm处进行双波段拉曼光谱。该系统可以自动测量培养皿或孔板中的样品。这个带有人工智能软件的系统有望用于识别食源性细菌的种类。摘要为实现食品安全检测的自动化和智能化,研制了一种新型的多模态光学传感系统。该系统采用785 nm和1064 nm的两对紧凑点激光器和色散光谱仪实现双波段拉曼光谱和成像,分别适用于测量产生低荧光和高荧光干涉信号的样品。自动光谱采集可以使用直接驱动的XY移动平台,将固体、粉末和液体样品放置在定制的孔板中或随机分散在标准培养皿中(例如,细菌菌落)。三个LED灯(白色背光、UV环光和白色环光)和两个微型彩色相机用于皮氏培养皿中样品的机器视觉测量,使用不同的照明和成像模式组合(例如,透射、荧光和彩色)。实时图像处理和运动控制技术用于实现自动样本计数,定位,采样和同步功能。系统软件采用集成人工智能功能的LabVIEW开发,能够即时识别和标记感兴趣的目标。通过对5种常见食源性细菌(包括蜡样芽孢杆菌、大肠杆菌、单核增生李斯特菌、金黄色葡萄球菌和沙门氏菌)的快速鉴定,验证了该系统的功能。利用基于线性支持向量机的机器学习模型,对生长在90 mm培养皿中的5种细菌222个菌落的拉曼光谱进行自动采集,分类准确率达到98.6%。整个系统建立在30×45 cm2面包板上,使其紧凑便携,可用于监管和工业应用中的现场和现场生物和化学食品安全检查。关键词:人工智能,自动采样,细菌,食品安全,机器学习,机器视觉,拉曼,传感
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引用次数: 1
Calibration and Validation of RZWQM2-P Model to Simulate Phosphorus Loss in a Clay Loam Soil in Michigan RZWQM2-P模型模拟密歇根州黏壤土磷流失的定标与验证
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15283
Md Sami Bin Shokrana, E. Ghane, Z. Qi
Highlights RZWQM2-P was tested and validated for clay loam soil using daily discharge and load data. The model performed satisfactorily in predicting hydrology and TP load, but DRP prediction was unsatisfactory. Inability of the model to simulate P loss in subsurface drainage discharge after fertilization event was one of the reasons for the unsatisfactory model performance. Abstract. Phosphorus (P) loss and transport through subsurface drainage systems is a primary focus for addressing harmful algal blooms in freshwater systems. The recent development of the phosphorus (P) routine of the Root Zone Water Quality Model (RZWQM2-P) has the potential to enhance our understanding of the fate and transport of P from subsurface-drained fields to surface water. However, there is a need to test the model under different fertilization, soil, climate, and cropping conditions. The objective of this study was to test the model's performance with daily drainage discharge, dissolved reactive phosphorus (DRP), and total phosphorus (TP) load collected from a subsurface-drained field with clay loam soil. We calibrated RZWQM2-P using two years of measured data. Subsequently, we validated RZWQM2-P using a year and nine months of measured data. We used the Nash-Sutcliffe model efficiency (NSE) and percentage bias (PBIAS) statistics for the RZWQM2-P model evaluation. The results showed that the model performance was “good” (daily NSE = 0.66 and PBIAS = -7.16) in predicting hydrology for the calibration period. For the validation period, the hydrology prediction of the model was “very good” (daily NSE = 0.76), but it had a “satisfactory” underestimation bias (PBIAS = 23.57). The model’s performance was “unsatisfactory” in simulating DRP for both calibration (daily NSE = 0.31 and PBIAS = -61.50) and validation (daily NSE = 0.32 and PBIAS = 43.68) periods. The P model showed “satisfactory” performance in predicting TP load for both calibration (daily NSE = 0.46 and PBIAS = -32.41) and validation (daily NSE = 0.39 and PBIAS = 42.90) periods, although both periods showed “unsatisfactory” percent bias. The underperformance may have been due to the model’s inability to partition fertilizer P into different P pools under high water tables or ponding conditions when using daily data. In conclusion, the RZWQM2-P model performed well for drainage discharge with daily data, but further investigation is needed to improve the P component of the model. Keywords: Field-scale modeling, Nutrient load, Phosphorus modeling, Subsurface drainage, Tile drainage, Water Quality.
RZWQM2-P在粘土壤土上进行了测试和验证,使用了每日排放和负荷数据。该模型对水文和TP负荷的预测效果较好,但对DRP的预测效果不理想。模型不能模拟施肥事件后地下排水的磷损失是模型性能不理想的原因之一。摘要磷(P)通过地下排水系统的损失和运输是解决淡水系统中有害藻华的主要重点。根区水质模型(RZWQM2-P)中磷(P)规律的最新发展有可能增强我们对地下排水田中磷的命运和向地表水的输送的认识。然而,有必要在不同施肥、土壤、气候和种植条件下对该模型进行测试。本研究的目的是通过从地下排水的粘土壤土中收集的日排水量、溶解活性磷(DRP)和总磷(TP)负荷来测试该模型的性能。我们使用两年的测量数据校准RZWQM2-P。随后,我们使用一年零九个月的测量数据验证了RZWQM2-P。我们使用Nash-Sutcliffe模型效率(NSE)和百分比偏差(PBIAS)统计对RZWQM2-P模型进行评估。结果表明,该模型在预测定标期水文方面表现“良好”(日NSE = 0.66, PBIAS = -7.16)。在验证期内,该模型的水文预测“非常好”(日NSE = 0.76),但存在“令人满意”的低估偏差(PBIAS = 23.57)。在校准(每日NSE = 0.31, PBIAS = -61.50)和验证(每日NSE = 0.32, PBIAS = 43.68)期间,模型在模拟DRP方面的表现都“不令人满意”。在校正期(每日NSE = 0.46, PBIAS = -32.41)和验证期(每日NSE = 0.39, PBIAS = 42.90), P模型在预测TP负荷方面表现出“令人满意”的表现,尽管这两个阶段都显示出“不令人满意”的百分比偏差。在使用日常数据时,由于模型无法在高地下水位或积水条件下将肥料磷划分为不同的磷池,因此表现不佳。综上所述,RZWQM2-P模型对日数据的排水流量表现良好,但需要进一步研究改进模型的P分量。关键词:田间模拟,养分负荷,磷模拟,地下排水,瓦排水,水质
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引用次数: 1
Predictor Selection and Machine Learning Regression Methods to Predict Saturated Hydraulic Conductivity From a Large Public Soil Database 预测器选择和机器学习回归方法从大型公共土壤数据库预测饱和水力传导性
4区 农林科学 Q3 AGRICULTURAL ENGINEERING Pub Date : 2023-01-01 DOI: 10.13031/ja.15068
Toby A. Adjuik, S. Nokes, M. Montross, M. Sama, O. Wendroth
Highlights In this study, six machine learning (ML) models were developed using a large database of soils to predict saturated hydraulic conductivity of these soils using easily measured soil characteristics. Tree-based regression models outperformed all other ML models tested. Neural networks were not suitable for predicting saturated hydraulic conductivity. Clay content, followed by bulk density, explained the highest amount of variation in the data of the predictors examined. Abstract. One of the most important soil hydraulic properties for modeling water transport in the vadose zone is saturated hydraulic conductivity. However, it is challenging to measure it in the field. Pedotransfer Functions (PTFs) are mathematical models that can predict saturated hydraulic conductivity (Ks) from easily measured soil characteristics. Though the development of PTFs for predicting Ks is not new, the tools and methods used to predict Ks are continuously evolving. Model performance depends on choosing soil features that explain the largest amount of Ks variance with the fewest input variables. In addition, the lack of interpretability in most “black box” machine learning models makes it difficult to extract practical knowledge as the machine learning process obfuscates the relationship between inputs and outputs in the PTF models. The objective of this study was to develop a set of new PTFs for predicting Ks using machine learning algorithms and a large database of over 8000 soil samples (the Florida Soil Characterization Database) while incorporating statistical methods to inform predictor selection for the model inputs. Of the machine learning (ML) models tested, random forest regression (RF) and gradient-boosted regression (GB) gave the best performances, with R2 = 0.71 and RMSE = 0.47 cm h-1 on the test data for both. Using the permutation feature importance technique, the GB and RF regression models showed similar results, where clay content described the most variation in the data, followed by bulk density. The implication of this study is that, when predicting Ks using the Florida Soil Characterization Database, priority should be given to obtaining quality data on clay content and bulk density as they are the most influential predictors for estimating Ks. Keywords: Deep learning, Gradient boosted regression, Pedotransfer functions, Random forest regression, Soil database, Soil properties.
在这项研究中,利用一个大型土壤数据库开发了六个机器学习(ML)模型,利用易于测量的土壤特征来预测这些土壤的饱和水力传导性。基于树的回归模型优于所有其他测试的ML模型。神经网络不适合预测饱和水导率。粘土含量,其次是体积密度,解释了所检查的预测数据中最大的变化。摘要其中一个最重要的土壤水力性质的模拟水在渗透带是饱和水力传导性。然而,在现场测量它是具有挑战性的。土壤传递函数(PTFs)是一种数学模型,可以根据容易测量的土壤特性预测饱和水力传导率(Ks)。虽然用于预测k的ptf的发展并不新鲜,但用于预测k的工具和方法仍在不断发展。模型的性能取决于选择用最少的输入变量解释最大数量的k方差的土壤特征。此外,大多数“黑箱”机器学习模型缺乏可解释性,这使得提取实用知识变得困难,因为机器学习过程模糊了PTF模型中输入和输出之间的关系。本研究的目的是开发一套新的ptf,用于使用机器学习算法和超过8000个土壤样本的大型数据库(佛罗里达土壤特征数据库)来预测k,同时结合统计方法来为模型输入的预测器选择提供信息。在测试的机器学习(ML)模型中,随机森林回归(RF)和梯度增强回归(GB)的性能最好,两者的测试数据的R2 = 0.71, RMSE = 0.47 cm h-1。使用排列特征重要性技术,GB和RF回归模型显示了相似的结果,其中粘土含量描述了数据中最大的变化,其次是体积密度。本研究的含义是,当使用佛罗里达土壤特征数据库预测k时,应优先考虑获得粘土含量和容重的高质量数据,因为它们是估计k的最具影响力的预测因子。关键词:深度学习,梯度增强回归,土壤传递函数,随机森林回归,土壤数据库,土壤性质
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引用次数: 0
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