Pub Date : 2018-11-18DOI: 10.33158/ASB.2018V4I2P93
D. J. Bertoncelli, Guilherme Augusto Cito Alves, Gustavo Henrique Freiria, F. Furlan, H. F. I. Neto, R. T. Faria
In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.
体外培养是兰花育苗生产中广泛采用的一种重要的生物技术方法，但由于不同品种的营养需求不同，有必要研究不同配方中所使用的营养成分的适宜性。目的是评价不同铁浓度对青苗离体培养的影响。幼苗是由离体萌发的种子获得的。采用改良MS培养基，常量营养物质浓度减半。微量营养素均按原配方添加，除铁从0.0的FeEDTA (FeSO4.7H2O: 5.6 g L-1, EDTA: 7.48 g L-1)原液中添加外;2.5;5.0;7.5;10.0和12.5mL L-1。在移栽200 d后，测定其茎高、根长、叶数、茎数、叶长、叶宽、地上和根干质量、叶绿素a、b和类胡萝卜素含量。试验采用完全随机设计，6个处理，10个重复，5个苗木。回归分析在5%显著性下进行。铁浓度的增加导致根长减少，叶和芽数增加。以4.13 mL L-1的FeEDTA浓度为最佳培养条件。高浓度的铁降低了幼苗的初始发育，但刺激了芽数的增加。
{"title":"Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa","authors":"D. J. Bertoncelli, Guilherme Augusto Cito Alves, Gustavo Henrique Freiria, F. Furlan, H. F. I. Neto, R. T. Faria","doi":"10.33158/ASB.2018V4I2P93","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I2P93","url":null,"abstract":"In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121151658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-18DOI: 10.33158/asb.2018v4i2p52
M. E. Escanferla, P. T. Wysmierski, W. F. Meirelles, L. Paccola-Meirelles
Maize white spot (MWS) is a disease widely spread in maize production regions in Brazil and causes serious economic damages to the culture. Little is known about the dissemination, growth and development conditions of the MWS causal agent, the bacterium Pantoea ananatis. The objective of this work was to determine the viability of this bacterium, as well as its distribution in the plant. P. ananatis after isolated was stored in both, Nutrient Broth (NB) medium and in mineral oil. For the viability tests, the cultures stored in NB were maintained at 12ºC and –6ºC and the cultures stored in mineral oil were maintained at room temperature. The isolate remained viable for seven months in NB medium at the two temperatures assessed, and four months when stored in mineral oil. The presence of the P. ananatis bacteria was analyzed in, 1) soil samples collected close to rhizosphere, 2) in seeds from plants severely attacked by MWS, 3) foliar segments of healthy plants and 4) the stem of healthy plants. P. ananatis was found surviving epiphytically on the leaf surface and its population increased with plant age, air relative humidity, and decreasing of temperature. This agent was not found neither rhizosphere nor in seeds, but it was isolated in low quantity in maize stem. P. ananatis resides epiphytically on leaves and, due to several factors, may trigger injuries to its host. All epiphytic isolates were characterized in regards to ice nucleation activity, a characteristic of this pathogen.
{"title":"Viability and dissemination of Pantoea ananatis, etiological agent of Maize White Spot disease","authors":"M. E. Escanferla, P. T. Wysmierski, W. F. Meirelles, L. Paccola-Meirelles","doi":"10.33158/asb.2018v4i2p52","DOIUrl":"https://doi.org/10.33158/asb.2018v4i2p52","url":null,"abstract":"Maize white spot (MWS) is a disease widely spread in maize production regions in Brazil and causes serious economic damages to the culture. Little is known about the dissemination, growth and development conditions of the MWS causal agent, the bacterium Pantoea ananatis. The objective of this work was to determine the viability of this bacterium, as well as its distribution in the plant. P. ananatis after isolated was stored in both, Nutrient Broth (NB) medium and in mineral oil. For the viability tests, the cultures stored in NB were maintained at 12ºC and –6ºC and the cultures stored in mineral oil were maintained at room temperature. The isolate remained viable for seven months in NB medium at the two temperatures assessed, and four months when stored in mineral oil. The presence of the P. ananatis bacteria was analyzed in, 1) soil samples collected close to rhizosphere, 2) in seeds from plants severely attacked by MWS, 3) foliar segments of healthy plants and 4) the stem of healthy plants. P. ananatis was found surviving epiphytically on the leaf surface and its population increased with plant age, air relative humidity, and decreasing of temperature. This agent was not found neither rhizosphere nor in seeds, but it was isolated in low quantity in maize stem. P. ananatis resides epiphytically on leaves and, due to several factors, may trigger injuries to its host. All epiphytic isolates were characterized in regards to ice nucleation activity, a characteristic of this pathogen.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133579622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-18DOI: 10.33158/ASB.2018V4I1P28
R. Koyama, A. M. Assis, W. F. S. Borges, L. Yamamoto, R. Colombo, D. Zeffa, L. Barros, Beatriz Barreira, Ibrar Shahab, Saeed Ahmed, S. Roberto
The objective of this study was to evaluate the influence of length of herbaceous cuttings and substrates on the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings. The experimental design was entirely randomized. Four replicates of 10 cuttings per plot in a 3x2 factorial arrangement, three cutting lengths (5, 8, and 12 cm) and two types of substrate (rice hull ash and coconut fiber) were evaluated. The cuttings were collected in two seasons: summer and fall. After the cuttings were prepared, they were placed in perforated plastic boxes containing each substrate for rooting, which were placed in a mist chamber under intermittent mist. After 158 days, the following variables were evaluated: proportion of rooted cuttings, cutting survival, leaf retention, number of roots per cutting, root mean length, root dry mass, unrooted cuttings with callus, and the proportion of sprouting cuttings. The use of 5-cm herbaceous cuttings collected in the summer and grown in rice hull optimizes the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings.
{"title":"Multiplication of blueberry mini-cuttings in different growth media","authors":"R. Koyama, A. M. Assis, W. F. S. Borges, L. Yamamoto, R. Colombo, D. Zeffa, L. Barros, Beatriz Barreira, Ibrar Shahab, Saeed Ahmed, S. Roberto","doi":"10.33158/ASB.2018V4I1P28","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P28","url":null,"abstract":"The objective of this study was to evaluate the influence of length of herbaceous cuttings and substrates on the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings. The experimental design was entirely randomized. Four replicates of 10 cuttings per plot in a 3x2 factorial arrangement, three cutting lengths (5, 8, and 12 cm) and two types of substrate (rice hull ash and coconut fiber) were evaluated. The cuttings were collected in two seasons: summer and fall. After the cuttings were prepared, they were placed in perforated plastic boxes containing each substrate for rooting, which were placed in a mist chamber under intermittent mist. After 158 days, the following variables were evaluated: proportion of rooted cuttings, cutting survival, leaf retention, number of roots per cutting, root mean length, root dry mass, unrooted cuttings with callus, and the proportion of sprouting cuttings. The use of 5-cm herbaceous cuttings collected in the summer and grown in rice hull optimizes the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125510596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-18DOI: 10.33158/ASB.2018V4I1P36
M. Avanzi, L. S. Matsumoto, U. Albino, Janaína Emiliano, G. Liuti, M. F. L. Andreata, M. Dealis, E. Niekawa, M. Navarro, G. Andrade
O uso de herbicidas no Brasil tem aumentado nos últimos anos, juntamente com a preocupação com o efeito que esses produtos podem causar no meio ambiente. O objetivo deste trabalho foi avaliar o efeito do herbicida sulfosato sobre grupos funcionais de microrganismos dos ciclos N e C na rizosfera de soja. O experimento foi conduzido em casa de vegetação, em delineamento experimental inteiramente casualizado, com cinco repetições. Os tratamentos incluíram um controle sem aplicação de herbicida e aplicação de N-fosfonometilglicina trimetilsulfônico (sulfosato) nas doses de 0,96 e 1,92 kg ha-1. Foram avaliadas as populações de bactérias heterotróficas, fungos saprófitas, actinomicetos, Pseudomonas fluorescens, celulolíticas, amilolíticas, proteolíticas e fixadoras de nitrogênio de vida livre. A massa seca de raiz e broto, número de nódulos e massa seca foram avaliados nas plantas. Na dose de 1,92 kg ha-1 de sulfosato, um efeito inibitório apareceu nas populações de fungos e actinomicetos. O herbicida sulfosato não afetou o crescimento das plantas e a nodulação em ambas as doses. A análise das correlações entre populações de microrganismos e entre populações de microrganismos e atributos de plantas mostrou diferenças entre os tratamentos herbicidas e as plantas controle.
近年来，巴西除草剂的使用有所增加，同时人们担心这些产品可能对环境造成的影响。本研究的目的是评价除草剂对大豆根际N和C循环微生物官能团的影响。试验在温室中进行，采用完全随机设计，5个重复。处理包括不施用除草剂的对照和施用N-磷甲基甘氨酸三甲基磺酸(磺酸)，剂量分别为0.96和1.92 kg hm - 2。对异养细菌、腐生真菌、放线菌、荧光假单胞菌、纤维素水解菌、淀粉水解菌、蛋白水解菌和自由生活固氮菌的种群进行了评价。测定了植株的根、芽干质量、节数和干质量。在1.92 kg hm -1的剂量下，对真菌和放线菌有抑制作用。两种剂量均不影响植物生长和结瘤。微生物居群之间以及微生物居群与植物属性之间的相关性分析表明，除草剂处理与对照植物之间存在差异。
{"title":"Impact of sulfosate on functional groups of microorganisms of the C and N cycles in the soybean rhizosphere","authors":"M. Avanzi, L. S. Matsumoto, U. Albino, Janaína Emiliano, G. Liuti, M. F. L. Andreata, M. Dealis, E. Niekawa, M. Navarro, G. Andrade","doi":"10.33158/ASB.2018V4I1P36","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P36","url":null,"abstract":"O uso de herbicidas no Brasil tem aumentado nos últimos anos, juntamente com a preocupação com o efeito que esses produtos podem causar no meio ambiente. O objetivo deste trabalho foi avaliar o efeito do herbicida sulfosato sobre grupos funcionais de microrganismos dos ciclos N e C na rizosfera de soja. O experimento foi conduzido em casa de vegetação, em delineamento experimental inteiramente casualizado, com cinco repetições. Os tratamentos incluíram um controle sem aplicação de herbicida e aplicação de N-fosfonometilglicina trimetilsulfônico (sulfosato) nas doses de 0,96 e 1,92 kg ha-1. Foram avaliadas as populações de bactérias heterotróficas, fungos saprófitas, actinomicetos, Pseudomonas fluorescens, celulolíticas, amilolíticas, proteolíticas e fixadoras de nitrogênio de vida livre. A massa seca de raiz e broto, número de nódulos e massa seca foram avaliados nas plantas. Na dose de 1,92 kg ha-1 de sulfosato, um efeito inibitório apareceu nas populações de fungos e actinomicetos. O herbicida sulfosato não afetou o crescimento das plantas e a nodulação em ambas as doses. A análise das correlações entre populações de microrganismos e entre populações de microrganismos e atributos de plantas mostrou diferenças entre os tratamentos herbicidas e as plantas controle.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133847745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-18DOI: 10.33158/ASB.2018V4I1P46
Geniane Lopes Carvalho Ozelame, Luana Borges Affonso, Thaís Helena Cappellaro, M. Schuch, Zeni Fonseca Pinto Tomaz
The expansion of Brazilian cherry tree cultivation is affected by propagation difficulties and slow seedling production development. The cuttings and minicuttings technique is an alternative that offers several advantages, and it proved to be highly promising for many fruit tree species. Minicuttings taken from a clonal garden were rooted in 22 x 14 x 10 cm plastic boxes containing vermiculite, at IAB concentration of 2.000 mg.L-1. The experimental design was totally randomized with eight treatments (cultivation weeks), with four replications, with five minicuttings each. They were evaluated eight times (once a week), for a period of two months. The following variables were analyzed: number of swollen minicuttings, rooted minicuttings and minicuttings with roots longer than 1 cm. From the third week onwards, rooting was positive, with 70% of rooted minicuttings and 5% with swellings. In the fourth evaluation, 55% already showed roots longer than one centimeter, with a mean of 2.66 cm, five with roots emergence and three with swellings. In general, in seven weeks’ time the minicuttings showed efficient root development and were ready to be transplanted to plastic bags to develop quality seedlings.
{"title":"Rooting dynamics of Brazilian cherry tree minicuttings","authors":"Geniane Lopes Carvalho Ozelame, Luana Borges Affonso, Thaís Helena Cappellaro, M. Schuch, Zeni Fonseca Pinto Tomaz","doi":"10.33158/ASB.2018V4I1P46","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P46","url":null,"abstract":"The expansion of Brazilian cherry tree cultivation is affected by propagation difficulties and slow seedling production development. The cuttings and minicuttings technique is an alternative that offers several advantages, and it proved to be highly promising for many fruit tree species. Minicuttings taken from a clonal garden were rooted in 22 x 14 x 10 cm plastic boxes containing vermiculite, at IAB concentration of 2.000 mg.L-1. The experimental design was totally randomized with eight treatments (cultivation weeks), with four replications, with five minicuttings each. They were evaluated eight times (once a week), for a period of two months. The following variables were analyzed: number of swollen minicuttings, rooted minicuttings and minicuttings with roots longer than 1 cm. From the third week onwards, rooting was positive, with 70% of rooted minicuttings and 5% with swellings. In the fourth evaluation, 55% already showed roots longer than one centimeter, with a mean of 2.66 cm, five with roots emergence and three with swellings. In general, in seven weeks’ time the minicuttings showed efficient root development and were ready to be transplanted to plastic bags to develop quality seedlings.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125514106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. B. Lone, R. Colombo, Clandio Medeiros da Silva, Alex Takeshi Inagati, S. Roberto
O objetivo deste trabalho foi avaliar o enraizamento e brotação de estacas de pitaya (Hylocereus undatus) sob diferentes níveis de sombreamento. As tonalidades obtidas por tela foram: 37,8%, 49,6%, 86,3% e sob o sol. Foram analisadas as seguintes características: sobrevivência de estacas (%), brotação (%), número de brotações, comprimento médio dos brotos (cm), massa seca da parte aérea (g), estacas enraizadas (%), comprimento médio da raiz (cm) (mL), matéria seca da raiz (g) e concentração de clorofila aeb (mg g-1). As médias percentuais de sobrevivência foram entre 90 e 100%. As respostas para fotografar e secar a matéria seca foram quadráticas, com ponto máximo em 23,48% e 42,7% de sombreamento, respectivamente. Em relação ao enraizamento de estacas, as médias foram entre 80 e 95%. O volume radicular e a massa seca das raízes apresentaram redução linear de acordo com o nível de sombreamento. Os teores de clorofila aeb apresentaram aumento linear na função o nível de sombreamento. O sombreamento parcial (entre 23 e 42%) aumentou a porcentagem de estacas com brotações, o acúmulo de matéria seca sobre elas e o volume afetado e o acúmulo de matéria seca nas raízes de viveiros pitaya propagados por estacas.
{"title":"Shading levels in the development of dragon fruit (pitaya) nurseries","authors":"A. B. Lone, R. Colombo, Clandio Medeiros da Silva, Alex Takeshi Inagati, S. Roberto","doi":"10.33158/ASB.2018V4I1P8","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P8","url":null,"abstract":"O objetivo deste trabalho foi avaliar o enraizamento e brotação de estacas de pitaya (Hylocereus undatus) sob diferentes níveis de sombreamento. As tonalidades obtidas por tela foram: 37,8%, 49,6%, 86,3% e sob o sol. Foram analisadas as seguintes características: sobrevivência de estacas (%), brotação (%), número de brotações, comprimento médio dos brotos (cm), massa seca da parte aérea (g), estacas enraizadas (%), comprimento médio da raiz (cm) (mL), matéria seca da raiz (g) e concentração de clorofila aeb (mg g-1). As médias percentuais de sobrevivência foram entre 90 e 100%. As respostas para fotografar e secar a matéria seca foram quadráticas, com ponto máximo em 23,48% e 42,7% de sombreamento, respectivamente. Em relação ao enraizamento de estacas, as médias foram entre 80 e 95%. O volume radicular e a massa seca das raízes apresentaram redução linear de acordo com o nível de sombreamento. Os teores de clorofila aeb apresentaram aumento linear na função o nível de sombreamento. O sombreamento parcial (entre 23 e 42%) aumentou a porcentagem de estacas com brotações, o acúmulo de matéria seca sobre elas e o volume afetado e o acúmulo de matéria seca nas raízes de viveiros pitaya propagados por estacas.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132759824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the species Olea europaea L. the tissue culture techniques have not been sufficiently studied by the lack of efficient establishment of protocols for various cultivars. The objective of this work was to evaluate the effect of different culture media and sampling times of explants on in vitro establishment of six cultivars of olive (Ascolano 315, Leccino, Maria da Fé, Coratina, Arbequina and Frantoio). The work was divided into two experiments conducted in the Fruit Tree Propagation Laboratory, Crop Science Department, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Brazil. Stock plants of the olive cultivars maintained in semi- hydroponic system were used. In the first experiment, nodal segments were used to establish in vitro culture medium MO and WPM, consisting of salts and vitamins. In the second experiment four seasons to obtain the explants were evaluate (autumn, winter, spring and summer). The WPM medium promoted greater establishment of olive explants. There is a higher phenolic oxidation in olive explants collected in winter. Spring is indicated for collecting explants, therefore favors the in vitro cultivar Maria da Fé, while Ascolano 315 and Arbequina cultivars have a higher rate of in vitro establishment by collecting explants over the year
在欧罗巴油橄榄(Olea europaea L.)中，由于缺乏有效的栽培方案，组织培养技术还没有得到充分的研究。本研究的目的是评价不同培养基和外植体取样次数对6个橄榄品种(Ascolano 315、Leccino、Maria da f、Coratina、Arbequina和Frantoio)离体培养的影响。这项工作分为两个实验，在巴西佩洛塔斯联邦大学Eliseu Maciel农学学院作物科学系果树繁殖实验室进行。采用半水培栽培的橄榄品种的后备植株。第一个实验采用节段法分别建立由盐和维生素组成的MO和WPM离体培养基。在第二次试验中，分别评价了秋、冬、春、夏4个季节获得外植体的条件。WPM培养基促进了橄榄外植体的建立。冬收橄榄外植体的酚氧化程度较高。春季是收集外植体的季节，因此有利于Maria da f的离体培养，而Ascolano 315和Arbequina的离体培养率较高
{"title":"Olive tree in vitro establishment under different culture media and explant collection periods","authors":"Roseane Maidana Moreira, M. Schuch","doi":"10.33158/ASB.2018V4I1P1","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P1","url":null,"abstract":"In the species Olea europaea L. the tissue culture techniques have not been sufficiently studied by the lack of efficient establishment of protocols for various cultivars. The objective of this work was to evaluate the effect of different culture media and sampling times of explants on in vitro establishment of six cultivars of olive (Ascolano 315, Leccino, Maria da Fé, Coratina, Arbequina and Frantoio). The work was divided into two experiments conducted in the Fruit Tree Propagation Laboratory, Crop Science Department, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Brazil. Stock plants of the olive cultivars maintained in semi- hydroponic system were used. In the first experiment, nodal segments were used to establish in vitro culture medium MO and WPM, consisting of salts and vitamins. In the second experiment four seasons to obtain the explants were evaluate (autumn, winter, spring and summer). The WPM medium promoted greater establishment of olive explants. There is a higher phenolic oxidation in olive explants collected in winter. Spring is indicated for collecting explants, therefore favors the in vitro cultivar Maria da Fé, while Ascolano 315 and Arbequina cultivars have a higher rate of in vitro establishment by collecting explants over the year","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123497806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-18DOI: 10.33158/ASB.2018V4I1P22
Andrés Vargas, A. M. Assis, M. Nadal, M. Schuch, Vanussa Tunes
A eficácia na produção de plântulas de maracujá-amarelo (Passiflora edulis Sims e F. flavicarpa Deg) pode ser afetada pela germinação irregular, devido, principalmente, a um curto período de dormência das sementes, bem como ao uso do substrato errado. Assim, o objetivo deste trabalho foi avaliar o efeito de três temperaturas de armazenamento e três substratos durante o desenvolvimento inicial de plântulas de maracujazeiro amarelo. O experimento foi conduzido em casa de vegetação agrícola na Universidade Federal de Pelotas, RS. Após a retirada da mucilagem e secagem, as sementes foram acondicionadas em embalagens de papel Kraft e armazenadas a 5 ºC, 10 ºC e temperatura ambiente (18 ± 0,6 ºC) por 15 dias. Em seguida, eles foram cultivados em fibra de coco Amafibra®, casca de arroz carbonizada e S-10Beifort®. Sementes mantidas a 18 ºC apresentaram menor comprimento da raiz sem diferença entre os armazenados a 5 ºC. Não houve efeito da temperatura na emergência, comprimento da parte aérea, número de folhas e biomassa seca da parte aérea e das raízes. A maior porcentagem de germinação foi encontrada no substrato da casca de arroz carbonizada; no entanto, com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento das plantas foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo. com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento da planta foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo. com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento da planta foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo.
生产效率幼苗盅(西番莲小人flavicarpa Deg)可能影响的主要是不规则的,因为短时间内休眠的种子,以及使用的介质。因此,这项工作的目标是评估三储存温度和基质的影响在幼苗的早期发展的maracujazeiro黄色。试验在美国佩洛塔斯联邦大学(Universidade Federal de Pelotas, RS)的农业温室中进行，种子去除粘液干燥后，装入牛皮纸包装，在5℃、10℃和室温(18±0.6℃)下保存15天。然后他们种植椰子纤维Amafibra®,皮烧焦的大米和-10年代beifort®。18℃贮藏的种子根长较短，5℃贮藏的种子根长无差异。温度对出苗、茎长、叶数和茎和根的干生物量无影响。比例最大的是被烧焦的介质的大米;然而，除了较高的根长和茎和根的干生物量外，植物的发育在其他基质中较高。,使用温度储存10ºC, S - -10和椰子纤维Beifort®作为基板的出现和发展是最好的建议,改善原盅的黄色。除了最大长度和地上部分的生物量和根的根,就是发展的植物和其他有关。因此，在贮藏过程中使用10ºC的温度，以及椰子纤维和S-10 Beifort®作为基质是改善西番莲果出苗和早期发育的最佳建议。除根长较大、地上部和根干生物量较大外，植株在其他基质上的发育较高。,使用温度储存10ºC, S - -10和椰子纤维Beifort®作为基板的出现和发展是最好的建议,改善原盅的黄色。
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Pub Date : 2018-05-10DOI: 10.33158/ASB.2018V4I1P14
W. S. Ricce, S. Roberto, J. Tonietto, P. Caramori
Paraná is the fourth largest grape producing state in Brazil and the cultivation of Vitis vinifera L. cultivars for winemaking is expanding is several regions of the state. The objective of this work was to characterize the potential of wine grape production based on the Géoviticulture Multicriteria Climatic Classification System for Paraná. A 30-year database constituted of 21 IAPAR (Agronomic Institute of Paraná) meteorological stations and 455 rainfall stations from Instituto das Águas do Paraná (Paraná State Water Institute) generated the following climatic indexes: dryness index (DI), heliothermal index (HI) and cool night index (CI) for the periods of October to March and April to September. According to the results, the viticultural climate of some regions in the state of Paraná belongs to the climatic groups where are found several traditional wine-producing regions in the world, showing potential for the expansion of winemaking in the state. Viticultural climate, associated with latitude in Paraná and with thermal conditions for vine growing cycle all over the year, make production displacement possible in the West, North and Northeastern regions and the production of the best quality grapes for winemaking in the fall and winter period, due to the most favorable cold night index and the lowest rainfall volume. In the coldest regions of the state (Center, South and East), it is only possible to have one production cycle, since the risk of frosts prevents the exploration of grapes in different periods.
帕拉纳州是巴西第四大葡萄生产州，酿酒葡萄品种的种植在该州的几个地区正在扩大。这项工作的目的是表征酿酒葡萄生产潜力的基础上的g葡萄栽培多标准气候分类系统为paran。由21个帕拉纳农业研究所 (IAPAR)气象站和Instituto das Águas do paran国家水研究所(Instituto das Water Institute) 455个雨站点组成的30年数据库生成了10 - 3月和4 - 9月的干燥指数(DI)、日温指数(HI)和凉爽夜晚指数(CI)。根据结果，帕拉纳州一些地区的葡萄栽培气候属于世界上几个传统葡萄酒产区的气候群，显示出该州扩大葡萄酒酿造的潜力。葡萄栽培气候，与帕拉纳岛的纬度和全年葡萄生长周期的热条件有关，使得西部、北部和东北部地区的生产转移成为可能，并且由于最有利的寒夜指数和最低的降雨量，秋冬季节可以生产出最优质的葡萄用于酿酒。在该州最冷的地区(中部、南部和东部)，只能有一个生产周期，因为霜冻的风险阻止了不同时期的葡萄探索。
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