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Agronomic performance of wheat genotypes and the use of nitrogen doses 小麦基因型的农艺性能及氮素用量的利用
Pub Date : 2023-07-04 DOI: 10.33158/asb.r193.v9.2023
Iago Jardim Santos, I. Carvalho, Leonardo Cesar Pradebon, Murilo Vieira Loro, E. D. Port, Guilherme Mallmann, Dassaiévis Gonzatto Maciel, Pedro Felipe Lopes, Gabriel Carioli
This work aimed to evaluate the agronomic performance of wheat cultivars and to evaluate the use of nitrogen doses. Two experiments took place at Centro de Pesquisas e Resultados Agronômicos das Missões (CEPRAM), in the municipality of Entre-Ijuís, Rio Grande do Sul, Brazil. Experiment I took place in a randomized block experimental design, evaluating 22 wheat genotypes in four replications. Experiment II took place in a randomized block experimental design, organized in a factorial arrangement of 7 nitrogen doses x 2 wheat genotypes. The doses of nitrogen were 0, 25, 50, 75, 100, 125, 150 kg ha-1 and two wheat cultivars ORS Senna and TBIO Audaz, with 4 replications. In 10 plants, randomly collected, in each experimental unit were evaluated the morphological and productive variables. Performing Analysis of variance and Tukey's mean comparison test in both experiments. Applying principal component analysis and genetic dissimilarity in experiment I. The cultivar TBIO Noble showed productive and morphological characteristics that maximize the agronomic performance of wheat. There is genetic divergence between wheat genotypes for productive and morphological traits. Cultivar ORS Senna enhanced ear length and number of tillers at doses of 44 kg of N ha-1 and 48 kg of N ha-1, respectively.
本研究旨在评价小麦品种的农艺性能,并对氮素用量进行评价。在巴西南里奥格兰德州Entre-Ijuís市的研究结果中心Agronômicos das Missões (CEPRAM)进行了两项实验。试验1采用随机区组试验设计,在4个重复中评估22种小麦基因型。试验II采用随机区组试验设计,按7个氮剂量x 2个小麦基因型进行因子安排。施氮量分别为0、25、50、75、100、125、150 kg hm -1,选用ORS Senna和TBIO Audaz 2个小麦品种,共4个重复。在每个试验单元随机抽取10株植物,对其形态和生产指标进行评价。对两个实验进行方差分析和Tukey均值比较检验。试验一:利用主成分分析和遗传差异分析,TBIO Noble品种表现出最大限度提高小麦农艺性能的生产和形态特征。小麦各基因型在生产性状和形态性状上存在遗传差异。44 kg N hm -1和48 kg N hm -1处理可显著提高苜蓿的穗长和分蘖数。
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引用次数: 0
Classification of soybean cultivars by means of artificial neural networks 基于人工神经网络的大豆品种分类
Pub Date : 2023-05-31 DOI: 10.33158/asb.r186.v9.2023
João Victor Costa Carneiro Paixão, É. Matsuo, Ithalo Coelho de Sousa, M. Nascimento, Igor Silva Oliveira, A. F. Macedo, Gustavo Martins Santana
The cultivation of soy has an economic importance for the Brazilian agricultural scenario. The aim of this study was to establish a network architecture for the classification of soybean genotypes, by means of morphological characters measured in the juvenile phase of the plant, and finally to compare the results obtained through Artificial Neural Network (ANN) and Anderson Discriminant Analysis. The study analyzed plants of 10 conventional cultivars in the initial stages of development (V1, V2 and V3 stages). The experiment was carried out in a randomized block design with 5 replications, and the experimental unit was represented by 9 plants. The data were submitted to the Anderson Discriminant Analysis and multilayer Perceptron ANN, with 1 or 2 hidden layers. To analyze the homogeneity of the variance and covariance matrix, the Box’s M-Test was adopted in the Program R, at 5% significance level. An input layer, one or two hidden layers, and an output layer formed the ANN architecture. The 5-fold cross validation was used to verify the efficiency of the discriminant functions and also in the ANN analysis. Subsequently, the apparent error rate (AER) was obtained. Box’s M-Test indicated inhomogeneity in the variance and covariance matrices, which indicated the need to perform Anderson's Quadratic Discriminant Analysis. The ANNs presented lower apparent error rate when compared to the Anderson's Quadratic Discriminant Analysis and the artificial neural network with 1 hidden layer was sufficient to perform the classification of soybean cultivars.
大豆的种植对巴西农业具有重要的经济意义。本研究的目的是通过测定大豆幼嫩期的形态特征,建立大豆基因型分类的网络结构,并对人工神经网络(ANN)和安德森判别分析(Anderson Discriminant Analysis)的结果进行比较。本研究对10个常规品种发育初期(V1、V2和V3期)的植株进行了分析。试验采用随机区组设计,5个重复,每个试验单元以9株植物为代表。数据被提交给安德森判别分析和多层感知器ANN,有1或2个隐藏层。为了分析方差和协方差矩阵的齐性,程序R采用Box’s m检验,在5%显著性水平下。一个输入层、一个或两个隐藏层和一个输出层构成了人工神经网络的体系结构。5重交叉验证用于验证判别函数的效率,也用于人工神经网络分析。进而得到表观错误率(AER)。Box 's m检验表明方差和协方差矩阵不均匀,这表明需要进行安德森二次判别分析。与Anderson二次判别分析相比,人工神经网络的表观错误率较低,1隐层的人工神经网络足以对大豆品种进行分类。
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引用次数: 1
Analysis of morphological characters in soybean plants submitted to different levels of artificial shading 不同遮荫水平下大豆植株形态特征分析
Pub Date : 2023-05-31 DOI: 10.33158/asb.r185.v9.2023
Willian Daniel Reis Gontijo, É. Matsuo, A. B. Evaristo, P. Cecon, S. C. Ferreira, Marcely Araújo Martins Reis
Morphological characters of soybean can be influenced by shading which are occasionally caused in plants. Thus, this study aimed to evaluate the effect of different shading levels on epicotyl length, epicotyl diameter and height of soybean plants. The experiments were conducted in a greenhouse. When the plants reached the V2 development stage, measurements were taken of epicotyl length and epicotyl diameter in experiment I (conducted in autumn); and epicotyl length, epicotyl diameter, and plant height in experiments II (spring/summer) and III (summer). Each experiments were conducted in a randomized block design, in subdivided plots, with four repetitions. The plots were composed of four shading levels and the subplots were composed of eight conventional cultivars. Each experimental unit consisted of one plant grown in a 3 dm³ pot filled with soil and organic matter. The three characters evaluated in the experiment presented significant effect for the interaction cultivar x shading x sowing seasons. Epicotyl length, independent of the sowing season, increased as the shading level intensified. Both the shading level and the sowing season influenced the diameter of the epicotyl, in a general way, reducing the diameter as the shading level increased. With increasing shading rate there was an increase in plant height. Moreover, the cultivars formed statistically distinct groups for the three morphological characters evaluated.
植物间或发生的遮荫会影响大豆的形态特征。因此,本研究旨在评价不同遮荫水平对大豆植株上胚轴长度、上胚轴直径和上胚轴高度的影响。实验是在温室里进行的。当植株达到V2发育阶段时,在试验一(秋季)测量上胚轴长度和直径;上胚轴长度、上胚轴直径和株高在春/夏试验II和夏试验III中均有显著差异。每个实验采用随机分组设计,在细分的地块中进行,重复4次。样地由4个遮阳层组成,子样地由8个常规品种组成。每个实验单元包括一株植物生长在一个3立方厘米的装满土壤和有机物的花盆中。试验评价的3个性状在互作品种x遮荫x播种季节均有显著影响。上胚轴长度随遮荫程度的增强而增加,与播种季节无关。遮荫程度和播种季节对上胚轴直径均有影响,遮荫程度越高,上胚轴直径越小。随着遮阳率的增加,株高增加。此外,品种在统计上形成了不同的类群。
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引用次数: 1
Effects of thermotherapy and meristem culture techniques on macro and micronutrients content in elephant grass cultivars 热疗和分生组织培养技术对象草宏量和微量元素含量的影响
Pub Date : 2023-05-31 DOI: 10.33158/asb.r182.v9.2023
M. G. Karasawa, V. B. Tavares, J. C. Pinto, Francisco José Silva Lédo, A. V. Pereira, J. P. Pinto
Elephant grass is a tropical forage crop highly used in dairy cattle production, in Brazil. It has been getting special attention, because of its bioenergy potential, medicinal properties, and bioremediation profile. The aim of the present study is to investigate the effects of thermotherapy based on clonal cleaning methods and meristem culture on the mineral content of elephant grass (Cenchrus purpureus (Schumach.) Morrone). Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the following methods: thermotherapy (T) combined to meristem culture (MC), meristem culture and mature stems (control). The experiment assessed the mineral contents of phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S) at three cuttings, which were performed every 60 days, for 180 days. There was lack of effects from these methods on the mineral content of approximately 66% of the carried out assessments, standing out unanimity for all cultivars, methods, and most of the cuts. T+MC was the only method showing positive effect on P and Ca content, in all cuts, in the cultivars Taiwan A-147 and Pioneiro, respectively. There was clear negative effect of cleaning methods on P and Ca content, in all cuts, for cultivar Mineiro, and on Mg, for cultivar Taiwan A-147. These results, along with the positive effects observed in vegetative and nutritional parameters shown in other articles published in this Journal, show that the clonal cleaning methods are strongly recommended for cultivars with more than 15 years of ripe stem propagation.
象草是一种热带饲料作物,在巴西被广泛用于奶牛生产。由于其生物能源潜力、药用特性和生物修复特性,它已经得到了特别的关注。本研究旨在探讨无性系清洗法和分生组织培养热疗对象草(Cenchrus purpureus (Schumach.))矿物含量的影响。莫龙)。以“Mineiro”、“Taiwan A-147”和“pioneer iro”三个品种为研究对象,采用热疗(T)结合分生组织培养(MC)、分生组织培养和成熟茎培养(对照)的方法。试验测定了每60 d采3次岩屑中磷(P)、钾(K)、钙(Ca)、镁(Mg)和硫(S)的矿物含量,共180 d。在进行的评估中,这些方法对大约66%的矿物含量没有影响,突出了所有品种、方法和大多数切割的一致性。T+MC处理是唯一对台湾A-147和“先峰”品种的磷、钙含量有显著影响的处理方法。清洗方式对栽培品种“米内罗”和“台湾A-147”各砧木的磷、钙含量和镁含量均有明显的负向影响。这些结果,以及在该杂志发表的其他文章中观察到的营养和营养参数的积极影响,表明克隆清洗方法强烈推荐用于成熟茎繁殖超过15年的品种。
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引用次数: 0
Management tendencies and needs: a joint proposal to maximize soybean grain yield 管理趋势和需求:大豆籽粒产量最大化的联合建议
Pub Date : 2023-05-26 DOI: 10.33158/asb.r187.v9.2023
Leonardo Cesar Pradebon, I. Carvalho, J. Sangiovo, Murilo Vieira Loro, Victor Delino Barasuol Scarton, E. D. Port, Guilherme Mallmann, Guilherme Stasiak, Dassaiévis Gonzatto Maciel, Pedro Felipe Lopes, Gabriel Carioli
The objective of the study was to evidence efficient management strategies in order to maximize soybean grain yield. The experiments took place at Centro de Pesquisas e Resultados Agronômicos das Missões (CPRAM), located in Entre - Ijuís - Rio Grande do Sul, Brasil. Three experiments were carried out in a randomized block design with four replications per treatment. The experiments were based on soybean crop management. Soil cover with vetch enhanced soybean grain yield. The cultivar BRS 5804RR® presented the best productive performance in the arrangements of 7 and 11 seeds m-1. Artificial defoliation did not influence soybean grain yield.
该研究的目的是为了证明有效的管理策略,以最大限度地提高大豆籽粒产量。实验在巴西南里奥格兰德州中心(Ijuís -南里奥格兰德州)的研究结果中心Agronômicos das Missões (CPRAM)进行。3个试验采用随机区组设计,每个处理4个重复。试验以大豆作物管理为基础。紫薇覆盖可提高大豆籽粒产量。品种BRS 5804RR®在7粒和11粒m-1的排列中表现出最好的生产性能。人工落叶对大豆籽粒产量没有影响。
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引用次数: 1
Enhanced understanding of anthracnose resistance in Michigan Dark Red Kidney common bean cultivar 加深对密歇根暗红肾普通豆品种炭疽病抗性的认识
Pub Date : 2023-04-03 DOI: 10.33158/asb.r167.v9.2023
Rafaella Juliane Gonçalves, P. S. Vidigal Filho, Helio Souza Junior, M. C. Gonçalves‐Vidigal, G. F. Lacanallo, Mariana Vaz Bisneta
Anthracnose, caused by the fungus Colletotrichum lindemuthianum (Sacc. and Magnus) Briosi and Cavara, is a major disease affecting the common bean (Phaseolus vulgaris L.), potentially causing yield losses of up to 100%. Utilizing resistant cultivars is the most effective approach for disease control. This study aimed to characterize the genetic resistance of two cultivars, TU and Michigan Dark Red Kidney (MDRK), to anthracnose. Inheritance tests were performed on F2:3 families from the TU (R) × AND 277 (S) cross using C. lindemuthianum race 3, and on F2:3 families from the crosses TU (R) × Kaboon (S) and TU (R) × Perry Marrow (S) inoculated with race 39. Inoculation with three races of C. lindemuthianum showed that MDRK carried the allele of the Co-1 locus. However, inoculation of F2:3 families from the MDRK × TU cross with race 1545 revealed that MDRK's resistance to this race is conditioned by two dominant genes (Co-1 allele on Pv01 and another allele on Pv04). Segregation results from inheritance tests using F2:3 families with the TU resistant cultivar fitted to a 1RR:2RS:1SS ratio, indicating the presence of a single dominant gene in the TU cultivar. Both the Mesoamerican TU and the Andean MDRK cultivars represent valuable sources of resistance to C. lindemuthianum and can be incorporated into common bean breeding programs to enhance disease resistance.
炭疽病,由真菌炭疽菌引起。是一种影响普通豆(Phaseolus vulgaris L.)的主要病害,可能造成高达100%的产量损失。利用抗病品种是防治病害最有效的途径。本研究旨在鉴定两个品种TU和密歇根暗红肾(MDRK)对炭疽病的遗传抗性。对TU (R) ×与277 (S)杂交的F2:3家系进行遗传试验,对TU (R) × Kaboon (S)和TU (R) × Perry Marrow (S)接种的39种杂交的F2:3家系进行遗传试验。接种3个小种的MDRK携带Co-1位点的等位基因。然而,将MDRK × TU杂交的F2:3家族与1545小种接种后发现,MDRK对该小种的抗性是由Pv01上的Co-1等位基因和Pv04上的另一个等位基因决定的。利用F2:3家系对TU抗性品种进行遗传测试,分离结果符合1RR:2RS:1SS的比例,表明TU品种存在单一显性基因。中美洲的TU和安第斯的MDRK品种都代表了对C. lindemuthianum的抗性的宝贵来源,可以纳入普通的豆类育种计划,以增强抗病能力。
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引用次数: 0
Prediction and importance of predictors in approaches based on computational intelligence and machine learning 基于计算智能和机器学习的方法中的预测和预测器的重要性
Pub Date : 2023-03-08 DOI: 10.33158/asb.r179.v9.2023
Antônio Carlos da Silva Júnior, Waldênia Melo Moura, L. L. Bhering, Michele Jorge Silva Siqueira, W. G. Costa, M. Nascimento, C. Cruz
Machine learning and computational intelligence are rapidly emerging in plant breeding, allowing the exploration of big data concepts and predicting the importance of predictors. In this context, the main challenges are how to analyze datasets and extract new knowledge at all levels of research.  Predicting the importance of variables in genetic improvement programs allows for faster progress, carrying out an extensive phenotypic evaluation of the germplasm, and selecting and predicting traits that present low heritability and/or measurement difficulties. Although, simultaneous evaluation of traits provides a wide variety of information, identifying which predictor variable is most important is a challenge for the breeder. The traditional approach to variable selection is based on multiple linear regression. It evaluates the relationship between a response variable and two or more independent variables.  However, this approach has limitations regarding its ability to analyze high-dimensional data and not capture complex and multivariate relationships between traits. In summary, machine learning and computational intelligence approaches allow inferences about complex interactions in plant breeding. Given this, a systematic review to disentangle machine learning and computational intelligence approaches is relevant to breeders and was considered in this review. We present the main steps for developing each strategy (from data selection to evaluating classification/prediction models and quantifying the best predictor).  
机器学习和计算智能在植物育种中迅速兴起,允许探索大数据概念并预测预测因子的重要性。在这种情况下,主要的挑战是如何分析数据集,并在各个层面的研究中提取新的知识。预测遗传改良项目中变量的重要性可以加快进展,对种质进行广泛的表型评估,选择和预测具有低遗传力和/或测量困难的性状。虽然性状的同时评估提供了各种各样的信息,但确定哪个预测变量是最重要的对育种者来说是一个挑战。传统的变量选择方法是基于多元线性回归的。它评估一个响应变量与两个或多个自变量之间的关系。然而,这种方法在分析高维数据的能力方面存在局限性,并且不能捕获特征之间复杂和多元的关系。总之,机器学习和计算智能方法可以推断植物育种中复杂的相互作用。鉴于此,本综述考虑了对机器学习和计算智能方法的系统综述,这与育种有关。我们介绍了开发每种策略的主要步骤(从数据选择到评估分类/预测模型和量化最佳预测器)。
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引用次数: 0
Selection of alfalfa genotypes for dry matter yield and persistence with repeated measures 苜蓿干物质产量和持续性基因型的重复测定选择
Pub Date : 2023-03-08 DOI: 10.33158/asb.r177.v9.2023
Cristiano Ferreira de Oliveira, Jacqueline Enequio Souza, Michele Jorge Silva Siqueira, Antônio Carlos da Silva Júnior, Reinaldo Paula Ferreira, D. Vilela, C. Cruz
The biggest challenge in the alfalfa breeding program is to obtain cultivars with high persistence, high productivity, and adaptability. Therefore, studies about selection methods are necessary for the success of alfalfa breeding programs. This study aimed to evaluate dry matter yield and persistence in alfalfa for selecting genotypes, using appropriate statistical models for experiments with repeated measures. The experiment was conducted at Embrapa Southeast Livestock, in São Carlos, state of São Paulo, Brazil in a randomized blocks design, in plots subdivided in time, with three replicates. Eight genotypes were evaluated, and the agronomic trait evaluated was dry matter yield. The experiments in split-plots were used with two and three errors and generalized linear models with the following correlation structures: composite symmetry (CS), heterogeneous composite symmetry (HCS), auto regressive (AR), heterogeneous auto regressive (HAR), and variance components (VC). The best model was selected according to the lowest value of the Akaike Information Criterion (AIC), and three methodologies were used to identify the genotype with greater productivity and persistence: Average test for multiple comparisons, adaptability, and stability by multi-information, and similarity between genotype and ideotype. The interaction between genotypes and cuts was significant, demonstrating the existence of the different behavior of the alfalfa genotypes over the cuts. Different methodologies allowed to measure the average yield of the alfalfa genotype and the persistence over the cuts. PSB 4 genotype demonstrated promissory behavior in terms of productivity and persistence throughout the production cycle of alfalfa.
苜蓿育种计划面临的最大挑战是获得高持久性、高生产力和适应性的品种。因此,选择方法的研究是苜蓿育种计划成功的必要条件。本研究旨在利用适当的统计模型进行重复试验,评价苜蓿干物质产量和持久性,以选择基因型。实验在巴西圣保罗州奥卡洛斯州东南畜牧研究所进行,采用随机区组设计,按时间细分,重复3次。评价了8个基因型,农艺性状评价为干物质产量。实验采用二误差和三误差的分裂图和广义线性模型,相关结构为复合对称(CS)、异质复合对称(HCS)、自回归(AR)、异质自回归(HAR)和方差成分(VC)。根据赤池信息准则(Akaike Information Criterion, AIC)的最小值选择最佳模型,并采用多重比较的平均检验、多信息的适应性和稳定性以及基因型与理想型的相似性3种方法来鉴定具有较高生产力和持久性的基因型。基因型与切口间的交互作用显著,说明苜蓿基因型在切口上存在不同的行为。不同的方法允许测量苜蓿基因型的平均产量和切割后的持久性。psb4基因型在紫花苜蓿整个生产周期的生产力和持久性方面表现出预期的行为。
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引用次数: 0
Effect of fertilization with basalt powder and inoculation of Bradyrhizobium spp in common bean 玄武岩粉施肥与接种蚕豆缓生根瘤菌的效果
Pub Date : 2023-03-07 DOI: 10.33158/asb.r183.v9.2023
J. G. Ferreira, Helio Souza Junior, A. Scherer, Ellen Rúbia Diniz
The common bean is a legume capable of performing biological nitrogen fixation (BNF). The objective of this work was to evaluate the effect of the application of rock powder associated with inoculation of Bradyrhizobium spp. in isolation or associated on the relative chlorophyll index in common bean leaves. Two experiments were conducted at the Federal Institute of Paraná Campus (IF-PR) in Ivaiporã- PR with four treatments and four completely randomized replicates. The treatments were: Inoculation of seeds with Bradyrhizobium spp; Inoculation of Bradyrhizobium spp. with basalt application; basalt application and control. The chemical analysis of the soil was performed. The seeds were inoculated with MasterFix based on Bradyrhizobium spp. following the manufacturer's recommendations. The relative chlorophyll index (IRC) was evaluated after the opening of the second trifoliate leaf. The IRC in experiment 1 was evaluated at 23, 38 and 53 days after sowing. In experiment 2, the IRC of the plants was evaluated at 25 and 41 days after sowing. The significant effect of treatments at 38 and 53 DAP was verified in relation to IRC, when the plants were cultivated with application of rock powder associated or not with Bradyrhizobium spp. There were treatment effects in relation to IRC in plants inoculated with Bradyrhizobium spp. and inoculated with Bradyrhizobium spp associated with rock powder, when compared with basalt powder and control.  There was benefit of increased IRC in both treatments inoculated with Bradyrhizobium spp.
普通豆是一种能够进行生物固氮(BNF)的豆类。本研究旨在评价岩石粉与单独或联合接种缓生根瘤菌对普通豆叶片相对叶绿素指数的影响。在Ivaiporã- PR的Federal Institute of paranus Campus (IF-PR)进行了两项实验,包括四种处理和四个完全随机重复。处理方法为:用慢生根瘤菌接种种子;玄武岩接种缓生根瘤菌的研究玄武岩的应用与控制。对土壤进行了化学分析。种子接种MasterFix基于慢生根瘤菌属,按照制造商的建议。测定三叶草第二叶开叶后的相对叶绿素指数(IRC)。试验1分别在播后23、38和53 d评价IRC。试验2分别在播种后25天和41 d测定植株的IRC。在施用缓生根瘤菌和不施用缓生根瘤菌伴生岩粉的情况下,38和53 DAP处理对IRC均有显著影响。与玄武岩粉和对照相比,接种缓生根瘤菌和接种缓生根瘤菌伴生岩粉的植株对IRC均有显著影响。接种慢生根瘤菌两种处理均有提高IRC的效果。
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引用次数: 0
Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars 热疗和分生组织培养对象草品种饲料产量和营养价值的影响
Pub Date : 2023-02-28 DOI: 10.33158/asb.r176.v9.2023
M. G. Karasawa, Valdir Tavares Botega, J. Pinto, Francisco José Silva Lédo, A. V. Pereira, J. C. Pinto
Elephant grass plays important role in dairy cattle production and has received special attention due to its potential in bioenergy and phytoremediation, as well as to its medicinal properties. The aim was investigating the effects of thermotherapy and meristem culture on elephant grass (Cenchrus purpureus (Schumach.) Morrone) forage production and nutritional value. Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the methods: thermotherapy plus meristem culture, meristem culture and mature stems (control). The experiment assessed the tiller number (TN), tiller height (TH), number of leaves/tiller (NLT), leaf/stem ratio (LSR), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), at three cuts performed every 60 days for 180 days. It was observed beneficial influence on the evaluated traits, which indicated how cleaning methods work. Cultivar “Mineiro”, with more than 20 years of use, was more sensitive to the cleaning methods showing than cultivars “Taiwan A-147” (15 years of use) and “Pioneiro” (less than 10 years). It was observed that methods have affected the NT, TH, SDM, LSR, LDM, CP, and NDF. And, that the cultivars genotype effect in a different way the NT, TH, NLT, SDM, LSR, and CP. Overall, Cleaning methods produced an increase in the evaluated parameters over 100% for the cultivars Mineiro and Taiwan when compared with the traditional method and presented low or negative effect for Pioneiro cultivar This evidenced that the traditional propagation method affects forage production and quality over generations in vegetative propagated species. Basic biotechnology techniques such as meristem culture associated or not with thermotherapy can restore the productive potential being recommended for old asexually propagated cultivars with more than fifteen years of cultivation.
象草在奶牛生产中发挥着重要作用,由于其在生物能源和植物修复方面的潜力以及其药用特性而受到特别关注。目的是研究热疗和分生组织培养对象草(Cenchrus purpureus (Schumach))的影响。牧草生产和营养价值。以“米内罗”、“台湾A-147”和“先锋”为研究对象,采用热疗+分生组织培养、分生组织培养+成熟茎(对照)的方法。试验评价了分蘖数(TN)、分蘖高(TH)、叶/分蘖数(NLT)、叶/茎比(LSR)、粗蛋白质(CP)、酸性洗涤纤维(ADF)和中性洗涤纤维(NDF),每60 d切3次,共180 d。观察到对被评价性状的有益影响,这表明清洁方法是如何工作的。使用年限超过20年的品种“Mineiro”对清洗方法的敏感性高于“Taiwan A-147”(15年)和“pioneer iro”(不到10年)。观察到方法影响了NT、TH、SDM、LSR、LDM、CP和NDF。此外,NT、TH、NLT、SDM、LSR和CP对不同品种的影响不同。总体而言,清洗方法对米内罗和台湾品种的评价参数比传统方法提高了100%以上,而对富西罗品种的影响较低或为负。这表明传统繁殖方法对营养繁殖品种的产量和品质有影响。基本的生物技术,如分生组织培养与热疗相结合或不相结合,可以恢复生产潜力,被推荐用于栽培超过15年的无性繁殖的老品种。
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引用次数: 1
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Agronomy Science and Biotechnology
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