Bioelectricity最新文献

Background: It is widely accepted that the inevitable leakage of electrons from mitochondria during respiratory metabolism is managed intracellularly through a complex cascade involving the conversion and transport of reactive oxygen species (ROS) and antioxidant mechanisms. However, considering that electrons can travel through various media, including biomacromolecules, do leaked electrons from the electron transport chain necessarily undergo complicated intracellular disposal processes? Here, we demonstrate that leaked electrons may actually be transported out of the cell.

Methods: By expressing circularly permuted yellow fluorescent protein (cpYFP), a superoxide indicator, at the microtubules, actin, and integrin of human embryonic lung fibroblasts.

Results: All three corresponding transgenic strains emitted fluorescent light, illuminating an ROS pathway from mitochondria to the outer cell membrane. The fluorescent intensity was positively correlated with mitochondrial superoxide content and aerobic respiratory intensity but was independent of cytosolic ROS levels.

Conclusion: These results suggest that leaked electrons are transported out of the cell in their electron form. We anticipate that our findings will serve as a starting point for revising the understanding of ROS generation and homeostasis, implicating various physiological and pathological processes.

Introduction: Bioelectric properties of cells are an important aspect of development, regeneration, and cancer. Because of their relevance to the establishment and maintenance of tissue form and function, bioelectric patterns have been hypothesized to have a role in aging. However, no data on bioelectric patterns of the whole body of young and old individuals have been available.

Methods: We observed and quantified the bioelectrics of whole-body immortal (growing at 22°C) and aging mortal (growing at 10°C) cold-sensitive Hydra oligactis.

Results: We found that the membrane-voltage-sensitive dyes FluoVolt and VF2.1.Cl can be used to reveal large-scale patterns of cellular membrane resting voltage potentials in hydra. The consensus whole-body bioelectric atlas of immortal hydra shows a consistently depolarized foot and occasionally depolarized tentacles. Immortal hydra are, on average, more depolarized and exhibit less sharply defined bioelectric patterns than old mortal hydra. Immortal hydra have a sharper foot:central body ratio than old mortal hydra.

Conclusions: These data establish hydra as the first model system in which whole-body bioelectric imaging can be performed; the different bioelectric patterns of immortal versus old mortal hydra are consistent with a bioelectric component to the aging process and suggest a roadmap for using this model organism in antiaging therapeutic screens involving electroceuticals.

Purpose: This study investigates two electromagnetic field (EMF) modalities on cancer cells: near-infrared pulsating light (NIR) and hyperpolarized light (HPL).

Methods: PC3, MCF7, and HeLa were exposed to Bioptron (HPL, 40 mW/cm²) and VieLight NeuroPro (NIR, 4.59 mW/cm², 810 nm, 10 Hz) for 10-60 min, with intermittent breaks. Viability, cytoskeletal and mitochondrial morphology, ATP, and lactate levels were assessed.

Results: NIR primarily enhanced mitochondrial ATP production, while HPL induced early morphological changes. Both modalities showed exposure dependency on viability.

Conclusion: The analysis reveals distinct cellular responses to NIR and HPL, underscoring the importance of optimized protocols for effective EMF therapies.

Introduction: Feline injection-site sarcoma (FISS) can arise from various agents that induce inflammation in the tissue, such as the injection of vaccines, antibiotics, anti-inflammatories, or even sutures.

Patients and methods: In this retrospective study, 72 cats with FISS grade 1 to 3 located in the spine or costo-abdominal area were included. They were separated into two categories: (A) patients treated with surgery with clean margins alone, surgery plus orthovoltage radiotherapy, and surgery plus electrochemotherapy. And category (B), patients treated with surgery without clean margins, surgery plus orthovoltage radiotherapy, surgery plus electrochemotherapy, and surgery plus orthovoltage radiotherapy and electrochemotherapy. In all cases, surgery with adequate margins was intended. In the cases that the histopathology of the resected tissue showed suboptimal margins, orthovoltage radiotherapy was added after wound healing. In the cases where surgery was performed with insufficient margins, electrochemotherapy was performed in the resection bed during surgery, following the veterinary guidelines for electrochemotherapy.

Results and conclusion: Results showed that patients with clean margin resection who underwent surgery alone had no statistically significant improvement in overall survival (OS) or disease-free survival (DFS) when adding orthovoltage radiotherapy or electrochemotherapy.

Abstract:  Among patients who underwent surgery without clear margins, those treated with surgery alone had a statistically significant shorter DFS and OS. In contrast, patients who received adjuvant orthovoltage radiotherapy, electrochemotherapy, or a combination of both demonstrated improved DFS and OS. However, no statistically significant differences were observed between the various adjuvant treatment modalities.

Abstract:  In conclusion, when FISS cannot be resected without clean margins, the addition of orthovoltage radiotherapy or electrochemotherapy can provide equal benefits regarding the improvement of DFS and OS times.