ACS Applied Bio Materials最新文献

This study examines the relationship between chondroitin sulfate, proteinoids, and computational neuron models, with a specific emphasis on the Izhikevich neuron model. We investigate the effect of chondroitin sulfate-proteinoid complexes on the behavior and dynamics of simulated neurons. Through the use of computational simulations, we provide evidence that these biomolecular components have the power to regulate the responsiveness of neurons, the patterns of their firing, and the ability of their synapses to change within the Izhikevich architecture. The findings suggest that the interactions between chondroitin sulfate and proteinoid cause notable alterations in the dynamics of membrane potential and the timing of spikes. We detect adjustments in the features of neuronal responses, such as shifts in the thresholds for firing, alterations in spike frequency adaptation, and changes to bursting patterns. The findings indicate that chondroitin sulfate and proteinoids may have a role in precisely adjusting neuronal information processing and network behavior. This study offers valuable information about the complex connection between the many components of the extracellular matrix, protein-based structures, and the functioning of neurons. In addition, our analysis of the proteinoid-chondroitine system using game theory uncovers a significant Prisoner's Dilemma scenario. The system's inclination toward defection, due to the appeal of cheating and the significant penalty for cooperation, with a mean voltage of -9.19 mV, indicates that defective behaviors may prevail in the long term dynamics of these neuronal interactions.

The multiple enzymatic properties of the Au³⁺-modified metal-organic framework (Au³⁺-MOFs) have made it a functional catalytic system for antitumor treatment. However, in the face of insufficient catalytic substrates in tumor tissue, it is still impossible to achieve efficient treatment of tumors. Herein, Au³⁺-MOFs loaded with hyaluronic acid (HA)-modified calcium peroxide nanoparticles (CaO₂ NPs) were used to construct a nanozyme (Au³⁺-MOF/CaO₂/HA) for substrate self-supplied and parallel catalytic/calcium-overload-mediated therapy of cancer. Due to the specific targeted ability and retention (EPR) effect of the HA, the built nanozyme can effectively accumulate at the tumor site. Due to the oxidase-like (OXD) activity and peroxidase-like (POD) activity of Au³⁺-MOFs, superoxide radical anion (O₂^•-) and hydroxyl radicals (·OH) were cooperatively formed for parallel catalytic therapy (PCT) of cancer. Subsequently, CaO₂ NPs were decomposed to Ca²⁺, H₂O₂, and O₂ in the weak acidic environment of the tumor microenvironment (TME). Thus, self-supplementation of O₂ as well as H₂O₂ was achieved, alleviating the deficiency of Au³⁺-MOF nanozyme catalytic substrate. In addition, Ca²⁺ can lead to oxidative stress for tumor calcification and calcium-overload-mediated therapy (COMT) to promote tumor necrosis in vivo. An effective paradigm of tumor PCT/COMT therapy with a self-supplying substrate has been successfully established for considerably enhanced therapeutic efficacy.

A multifunctional nanoplatform integrating multiple therapeutic functions may be an effective strategy to realize satisfactory therapeutic efficacy in the treatment of tumors. However, there is still a certain challenge in integrating multiple therapeutic agents into a single formulation using a simple method due to variations in their properties. In this work, multifunctional CuS-ICG@PDA-FA nanoparticles (CIPF NPs) with excellent ability to produce reactive oxygen species and photothermal conversion performance are fabricated by a simple and gentle method. Hollow mesoporous copper sulfide nanoparticles (HMCuS NPs) not only have excellent loading and photothermal conversion performance but also can cause a highly efficient Fenton-like reaction for chemodynamic therapy (CDT). The loaded photosensitizer indocyanine green (ICG) imparts excellent photodynamic properties to the NPs, which in turn enhances the stability of ICG. The polydopamine (PDA) coating improves the stability and biocompatibility of the NPs and creates the conditions for surface modification of folic acid. The FA-coated NPs show precise targeting of tumor cells. The results of the cellular uptake assay demonstrate that CIPF NPs enter tumor cells through an endocytic pathway. Lysosome colocalization and escape experiments prove that CIPF NPs possess good lysosomal escape ability under irradiation of NIR. Both in vitro and in vivo antitumor studies of CIPF NPs reveal excellent efficacy in photothermal/photodynamic/chemodynamic therapy. The construction of high-performance CIPF NPs offers valuable insights into the design of a multifunctional copper sulfide-based nanoplatform for combined cancer treatment and precise theranostics.

Contemporary therapies following heart failure center on regenerative approaches to account for the loss of cardiomyocytes and limited regenerative capacity of the adult heart. While the delivery of cardiac progenitor cells has been shown to improve cardiac function and repair following injury, recent evidence has suggested that their paracrine effects (or secretome) provides a significant contribution towards modulating regeneration, rather than the progenitor cells intrinsically. The direct delivery of secretory biomolecules, however, remains a challenge due to their lack of stability and tissue retention, limiting their prolonged therapeutic efficacy. We hypothesized that polyurethane-based nanoparticles with heteropolar-hydrophobic-ionic chemistry (DPHI-NPs) could enable the delivery of a subset of pro-regenerative cardiac progenitor cell proteins [bone morphogenetic protein-4 (BMP-4) and angiotensin 1-7 (Ang1-7)] to promote biological pathways conducive to repair processes such as antisenescence (through the quantification of β-galactosidase and interleukin-6) and vasculogenesis (through the formation of endothelial tubes), demonstrated in vitro with human cardiac fibroblasts (hCFs) and human microvascular endothelial cells (hMECs), respectively. DPHI-NPs with a diameter of 190 ± 2 nm (polydispersity index < 0.2) and a zeta potential of -40 ± 1 mV were generated using an emulsion inversion technique and loaded with both therapeutic proteins (BMP-4 and Ang1-7) by optimizing surface charge, loading solution concentration, coating duration, and coating efficiency. Senescence-induced hCFs treated with functionalized DPHI-NPs were found to exhibit a significant reduction in expressed β-galactosidase and IL-6 (p < 0.05). Additionally, hMECs treated with NP_BMP-4 were found to display enhanced vasculogenesis compared to control culture conditions alone (p < 0.05). The development of a DPHI-NP vector for the delivery of pro-regenerative secretome biomolecules may present an effective translatable strategy to improve their therapeutic efficacy with respect to cell function.

Cyanogenic glycosides are plant-derived, nitrogen-containing secondary metabolites that release toxic cyanide ions upon hydrolysis by glycosidic enzymes. Therefore, consuming food items enriched with such compounds without proper remediation can cause acute cyanide intoxication. Thus, in this work, we utilize cyanide-responsive oxidized bisindole-based chromogenic probes to detect cyanogenic glycosides, such as amygdalin and linamarin (LOD: 0.12 μM), in phospholipid membranes. The bilayer surface, owing to its distinct microenvironment, enhances both the sensitivity and specificity of the probes toward amygdalin. The chromogenic response (red to yellow) is influenced by the nature of the lipid membrane (order, polarity, and interfacial hydration) as well as the number of bis-indolyl units in the probe molecules. Semiquantitative analysis of food samples before and after cooking revealed that soaking in water at room temperature significantly reduces the cyanogenic glycoside content. The ability to directly detect cyanogenic glycosides in food samples without pretreatment is a notable aspect of this investigation.

COVID-19 has become one of the deadliest epidemics in the past years. In efforts to combat the deadly disease besides vaccines, drug therapies, and facemasks, significant focus has been on designing specific methods for the sensitive and accurate detection of SARS-CoV-2. Of these, surface-enhanced Raman scattering (SERS) is an attractive analytical tool for the identification of SARS-CoV-2. SERS is the phenomenon of enhancement of Raman intensity signals from molecular analytes anchored onto the surfaces of roughened plasmonic nanomaterials. This work gives an updated summary of plasmonic gold nanomaterials (AuNMs) and silver nanomaterials (AgNMs)-based SERS technologies to identify SARS-CoV-2. Due to extreme "hot spots" promoting higher electromagnetic fields on their surfaces, different shapes of AuNMs and AgNMs combined with Raman probes have been reviewed for enhancing Raman signals of probe molecules for quantifying the virus. It also reviews progress made recently in the design of certain specific Raman probe molecules capable of imparting characteristic SERS response/tags for SARS-CoV-2 detection.

The synthesis of nanomaterials from renewable resources has emerged as an environmentally friendly alternative. This approach helps to reduce the use of chemical fertilizers in agricultural production, further reducing the potential harm to the ecosystem and effectively reducing the burden on the environment. In this work, we synthesized Rosa roxburghii derived carbon dots (CDs) using the microwave hydrothermal method (RR-CDs) and the electrolytic oxidation method (GRR-CDs), and the results showed that RR-CDs had a wider ultraviolet absorption range and emitted blue fluorescence. These properties make RR-CDs more effective as light-harvesting materials in plants, thus promoting photosynthesis. In the cultivation of lettuce, RR-CDs significantly enhanced both the biomass and the quality of the lettuce. In addition, compared to the control group, the chlorophyll content of lettuce treated with RR-CDs increased by 31.83%, the net photosynthetic rate increased by 60.76%, and the electron transport rate of photosystem II increased by 38.72%. Therefore, we found that the microwave hydrothermal method could bring better benefits, with a yield of up to 40.20% after just 2 h of reaction. RR-CDs promote photosynthesis by promoting light conversion and improving nutrient efficiency while also boasting the dual advantages of low cost and easy large-scale production, thus opening up avenues for sustainable agricultural production.

The Coronavirus Disease 2019 (COVID-19) recently emerged as a life-threatening global pandemic that has ravaged millions of lives. The affected patients are known to frequently register numerous comorbidities induced by COVID-19 such as diabetes, asthma, cardiac arrest, hypertension, and neurodegenerative diseases, to name a few. The expensiveness and probability of false negative results of conventional screening tests often delay timely diagnosis and treatment. In such cases, the deployment of a suitable biosensing platform can readily expedite the rapid diagnosis process for enhanced patient outcomes. We report the development of an electrochemical genosensor based on DNA/OGCN (DNA/oxygenated graphitic carbon nitride) nanohybrids for the quantification of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) DNA─the key biomarker for COVID-19. This is achieved by exploiting the molecular nanowire-formation capability of the [Ru(NH₃)₆]^2+/3+ redox probe onto the DNA phosphate backbone via electrostatic interactions. The microstructural characterization of OGCN was performed using scanning electron microscopy (SEM) coupled with an energy-dispersive X-ray (EDX) module, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy. The electrochemical analyses were performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), while the analytical performance of the sensor was evaluated using square wave voltammetry (SWV). The developed sensor exhibited a wide linear detection range within 10 fM-10 μM, with a limit of detection (LoD) of ∼7.23 fM with a high degree of selectivity toward SARS-CoV-2 target DNA, thereby indicating its potential to be employed in a point-of-care scenario toward providing affordable healthcare to the global populace.

Bioprinting of nanohydroxyapatite (nHA)-based bioinks has attracted considerable interest in bone tissue engineering. However, the role and relevance of the physicochemical properties of nHA incorporated in a bioink, particularly in terms of its printability and the biological behavior of bioprinted cells, remain largely unexplored. In this study, two bioinspired nHAs with different chemical compositions, crystallinity, and morphologies were synthesized and characterized: a more crystalline, needle-like Mg²⁺-doped nHA (N-HA) and a more amorphous, rounded Mg²⁺- and CO₃^2--doped nHA (R-HA). To investigate the effects of the different compositions and morphologies of these nanoparticles on the bioprinting of human bone marrow stromal cells (hBMSCs), gelatin and gelatin methacryloyl (GelMA) were selected as the bioink backbone. The addition of 1% (w/w) of these bioceramic nanoparticles significantly improved the printability of GelMA in terms of extrudability, buildability, and filament spreading. The biological potential of the bioinks was evaluated by examining the hBMSC viability, metabolic activity, and osteogenic differentiation over 21 days. Both nHAs showed high cell viability, with N-HA showing a significant increase in metabolic activity under nonosteogenic conditions and R-HA showing a notable increase with osteogenic stimulation. These results suggest that the two nHAs interact differently with their environment, highlighting the importance of both the chemistry and morphology in bioink performance. In addition, osteogenic differentiation further highlighted how the physicochemical properties of nHAs influence osteogenic markers at both the RNA and protein levels. Clearly, tailoring the physicochemical properties of hydroxyapatite nanoparticles is critical to developing more biomimetic bioinks with great potential for advancing bone bioprinting applications.