Pub Date : 2019-12-06DOI: 10.24265/horizmed.2019.v19n4.12
Jorge Luis Vélez, Mario Montalvo, Santiago Aguayo, P. Vélez, G. Velarde, F. E. Jara González, Joshuan Barboza-Meca
espanolEl glicocalix endotelial es una estructura sin forma definida que recubre la capa luminal del endotelio vascular y que esta constituido, principalmente, por tres elementos: proteoglicanos, glucosaminoglicanos y glicoproteinas. Cumple distintas funciones, como regular la permeabilidad vascular a las moleculas y liquidos, la transduccion de las fuerzas mecanicas de tension y las cascadas de fibrinolisis y coagulacion vascular; ademas, protege de la adhesion leucocitaria, plaquetaria y de patogenos. Los determinantes de lesion del glicocalix pueden ser de varios tipos, por ejemplo, incremento las fuerzas de tension, especies reactivas de oxigeno (O ), aumento, a nivel plasmatico, de sustancias como el sodio (hipernatremia), glucosa (hiperglicemia) y colesterol (hipercolesterolemia), y las moleculas proinflamatorias. Cualquiera de las noxas citadas, individualmente o combinadas, lesionan el glicocalix y la disfuncion resultante se expresara clinicamente como disfuncion endotelial, aumento de la permeabilidad vascular, paso de lipoproteinas al subendotelio, activacion de la coagulacion o aumento de la adhesion de plaquetas y leucocitos al endotelio. EnglishEndothelial glycocalyx is an undefined structure covering the luminal layer of the vascular endothelium and consisting mainly of three elements: proteoglycans, glycosaminoglycans and glycoproteins. It has different functions, such as the regulation of vascular permeability to liquids and molecules; transduction of the mechanical forces of vascular tension; regulation of coagulation and fibrinolysis cascades; and protection of leukocyte, platelet and pathogen adhesion. The determinants of a glycocalyx lesion can be of several types―e.g., increased tensile forces; reactive oxygen (O ) species; increased plasma level of substances such as sodium (hypernatremia), glucose (hyperglycemia) and cholesterol (hypercholesterolemia); and pro-inflammatory molecules. Any of the above-mentioned noxas, alone or combined, injure the glycocalyx. Its dysfunction will be clinically expressed as endothelial dysfunction, increased vascular permeability, filtration of lipoproteins to the subendothelium, activation of coagulation, or increased adhesion of leukocytes and platelets to the endothelium.
{"title":"Glicocálix endotelial: relevancia clínica y enfoque traslacional","authors":"Jorge Luis Vélez, Mario Montalvo, Santiago Aguayo, P. Vélez, G. Velarde, F. E. Jara González, Joshuan Barboza-Meca","doi":"10.24265/horizmed.2019.v19n4.12","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.12","url":null,"abstract":"espanolEl glicocalix endotelial es una estructura sin forma definida que recubre la capa luminal del endotelio vascular y que esta constituido, principalmente, por tres elementos: proteoglicanos, glucosaminoglicanos y glicoproteinas. Cumple distintas funciones, como regular la permeabilidad vascular a las moleculas y liquidos, la transduccion de las fuerzas mecanicas de tension y las cascadas de fibrinolisis y coagulacion vascular; ademas, protege de la adhesion leucocitaria, plaquetaria y de patogenos. Los determinantes de lesion del glicocalix pueden ser de varios tipos, por ejemplo, incremento las fuerzas de tension, especies reactivas de oxigeno (O ), aumento, a nivel plasmatico, de sustancias como el sodio (hipernatremia), glucosa (hiperglicemia) y colesterol (hipercolesterolemia), y las moleculas proinflamatorias. Cualquiera de las noxas citadas, individualmente o combinadas, lesionan el glicocalix y la disfuncion resultante se expresara clinicamente como disfuncion endotelial, aumento de la permeabilidad vascular, paso de lipoproteinas al subendotelio, activacion de la coagulacion o aumento de la adhesion de plaquetas y leucocitos al endotelio. EnglishEndothelial glycocalyx is an undefined structure covering the luminal layer of the vascular endothelium and consisting mainly of three elements: proteoglycans, glycosaminoglycans and glycoproteins. It has different functions, such as the regulation of vascular permeability to liquids and molecules; transduction of the mechanical forces of vascular tension; regulation of coagulation and fibrinolysis cascades; and protection of leukocyte, platelet and pathogen adhesion. The determinants of a glycocalyx lesion can be of several types―e.g., increased tensile forces; reactive oxygen (O ) species; increased plasma level of substances such as sodium (hypernatremia), glucose (hyperglycemia) and cholesterol (hypercholesterolemia); and pro-inflammatory molecules. Any of the above-mentioned noxas, alone or combined, injure the glycocalyx. Its dysfunction will be clinically expressed as endothelial dysfunction, increased vascular permeability, filtration of lipoproteins to the subendothelium, activation of coagulation, or increased adhesion of leukocytes and platelets to the endothelium.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"84-92"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49198417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.24265/horizmed.2019.v19n4.11
Carlos Barrionuevo Cornejo, Daniela Dueñas Hancco
In recent years, the rapid technological development and the better knowledge of genetic and molecular aspects in medicine have allowed a better approach to understand, diagnose and treat various oncological diseases. Regarding lung carcinoma, there has been a remarkable evolution from the first classifications, which were purely morphological, to the last one issued in 2015. This last classification includes histological, immunophenotypic, molecular genetics, clinical and radiological information, which allows a better evaluation and therapeutic management of these patients. In the present article, the first classifications of lung carcinoma to the last one are reviewed, and the most relevant changes and the importance of the molecular genetics findings are examined for a better clinical and therapeutic approach.
{"title":"Clasificación actual del carcinoma de pulmón. Consideraciones histológicas, inmunofenotípicas, moleculares y clínicas","authors":"Carlos Barrionuevo Cornejo, Daniela Dueñas Hancco","doi":"10.24265/horizmed.2019.v19n4.11","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.11","url":null,"abstract":"In recent years, the rapid technological development and the better knowledge of genetic and molecular aspects in medicine have allowed a better approach to understand, diagnose and treat various oncological diseases. Regarding lung carcinoma, there has been a remarkable evolution from the first classifications, which were purely morphological, to the last one issued in 2015. This last classification includes histological, immunophenotypic, molecular genetics, clinical and radiological information, which allows a better evaluation and therapeutic management of these patients. In the present article, the first classifications of lung carcinoma to the last one are reviewed, and the most relevant changes and the importance of the molecular genetics findings are examined for a better clinical and therapeutic approach.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"74-83"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44655295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.24265/horizmed.2019.v19n4.06
Rosario Margarita Yslado Méndez, R. Figueroa, T. Poma, Eva Zarzosa Marquez, L. Castro, Irene Pinto Flores, Angelita Rojas Gamboa
Objetivo: El objetivo del estudio es estimar las relaciones funcionales entre el burnout y la satisfaccion laboral de los profesionales de la salud de dos hospitales del Peru. Se aplico el “Inventario de burnout de Maslach” MBI y la “Escala de satisfaccion laboral (SL-SPC)” Materiales y metodos: Es un estudio cuantitativo, descriptivo correlacional y transversal. Se encuestaron 177 profesionales de la salud (medicos, odontologos, quimicos farmaceuticos, psicologos, enfermeros, obstetras, tecnologos medicos, nutricionistas y trabajadores sociales) Resultados: Mediante el analisis de ecuaciones estructurales se encontro que existe relacion significativa (AGFI = 0,946) y negativa (coeficiente estructural = -0,62) entre el burnout y la satisfaccion laboral en los profesionales de la salud; el burnout es predictor de la satisfaccion laboral. La dimension predominante del burnout fue la despersonalizacion (37%=0,72/ (0,56+0,72+0,65)); y el factor predominante de la satisfaccion laboral fueron los beneficios economicos (35%=0,99/ (0,67+0,99+0,48+0,66)). Conclusiones: Se propone realizar investigaciones futuras sobre las variables moderadoras, que explican la relacion entre el burnout y la satisfaccion laboral e implementar programas de intervencion ocupacional y organizacional.
{"title":"Síndrome de burnout y la satisfacción laboral en profesionales de la salud","authors":"Rosario Margarita Yslado Méndez, R. Figueroa, T. Poma, Eva Zarzosa Marquez, L. Castro, Irene Pinto Flores, Angelita Rojas Gamboa","doi":"10.24265/horizmed.2019.v19n4.06","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.06","url":null,"abstract":"Objetivo: El objetivo del estudio es estimar las relaciones funcionales entre el burnout y la satisfaccion laboral de los profesionales de la salud de dos hospitales del Peru. Se aplico el “Inventario de burnout de Maslach” MBI y la “Escala de satisfaccion laboral (SL-SPC)” Materiales y metodos: Es un estudio cuantitativo, descriptivo correlacional y transversal. Se encuestaron 177 profesionales de la salud (medicos, odontologos, quimicos farmaceuticos, psicologos, enfermeros, obstetras, tecnologos medicos, nutricionistas y trabajadores sociales) Resultados: Mediante el analisis de ecuaciones estructurales se encontro que existe relacion significativa (AGFI = 0,946) y negativa (coeficiente estructural = -0,62) entre el burnout y la satisfaccion laboral en los profesionales de la salud; el burnout es predictor de la satisfaccion laboral. La dimension predominante del burnout fue la despersonalizacion (37%=0,72/ (0,56+0,72+0,65)); y el factor predominante de la satisfaccion laboral fueron los beneficios economicos (35%=0,99/ (0,67+0,99+0,48+0,66)). Conclusiones: Se propone realizar investigaciones futuras sobre las variables moderadoras, que explican la relacion entre el burnout y la satisfaccion laboral e implementar programas de intervencion ocupacional y organizacional.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"41-49"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.24265/horizmed.2019.v19n4.08
Marco Antonio Alfaro Angulo, Ana Maria Ruth Canevaro Alva
Objective: To describe the characteristics of a scabies outbreak and assess the effect of the control measures. Materials and methods: A descriptive and prospective epidemiological study was conducted at a reference hospital with220 inpatient beds. Data was collected by visiting the Medicine Service on a daily basis, interviewing the healthcare personnel and examining the medical records. The description of the outbreak includes epidemiological variables such as subject, place and time. Descriptive statistics was used to determine the absolute and relative frequencies, as well as the attack rate. Patients and healthcare personnel were requested to sign an informed consent and received information about the benefits of the research. The intervention consisted of isolation measures and contact transmission-based precautions. The use of non-sterile gloves was emphasized. Results: The outbreak took place between the epidemiological weeks 29 and 37 in the year 2017. The index case was a 93-year-old male patient who had Norwegian scabies and was admitted to the hospital due to Alzheimer’s disease. The scabies occurred in nine female nurses, seven residents, six nurse technicians, one intern y five inpatients. Conclusions: This propagated-source outbreak lasted nine epidemiological weeks; and affected nurses, residents, interns and inpatients. The attack rate was 40 %. The prevention and control measures were isolation of the index case and patients, hand hygiene before and after patient care, use of non-sterile gloves, use of scrubs, use of individual medical supplies for each patient, handling of clothing from scabies-infected patients in sealed bags and using gloves, surface cleaning, withdrawal of scabies-infected health workers from their duties until 24 hours of treatment onset, and epidemiological surveillance. Medical treatment was 5 % permethrin lotion for three days. Use of biosafety standards is recommended for patient care, especially when patients are admitted to the hospital with skin lesions. The implemented measures must be considered to prevent and control this type of outbreak.
{"title":"Brote de escabiosis en el Hospital Víctor Lazarte Echegaray, Trujillo, 2017","authors":"Marco Antonio Alfaro Angulo, Ana Maria Ruth Canevaro Alva","doi":"10.24265/horizmed.2019.v19n4.08","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.08","url":null,"abstract":"Objective: To describe the characteristics of a scabies outbreak and assess the effect of the control measures. \u0000Materials and methods: A descriptive and prospective epidemiological study was conducted at a reference hospital with220 inpatient beds. Data was collected by visiting the Medicine Service on a daily basis, interviewing the healthcare personnel and examining the medical records. The description of the outbreak includes epidemiological variables such as subject, place and time. Descriptive statistics was used to determine the absolute and relative frequencies, as well as the attack rate. Patients and healthcare personnel were requested to sign an informed consent and received information about the benefits of the research. The intervention consisted of isolation measures and contact transmission-based precautions. The use of non-sterile gloves was emphasized. \u0000Results: The outbreak took place between the epidemiological weeks 29 and 37 in the year 2017. The index case was a 93-year-old male patient who had Norwegian scabies and was admitted to the hospital due to Alzheimer’s disease. The scabies occurred in nine female nurses, seven residents, six nurse technicians, one intern y five inpatients. \u0000Conclusions: This propagated-source outbreak lasted nine epidemiological weeks; and affected nurses, residents, interns and inpatients. The attack rate was 40 %. The prevention and control measures were isolation of the index case and patients, hand hygiene before and after patient care, use of non-sterile gloves, use of scrubs, use of individual medical supplies for each patient, handling of clothing from scabies-infected patients in sealed bags and using gloves, surface cleaning, withdrawal of scabies-infected health workers from their duties until 24 hours of treatment onset, and epidemiological surveillance. Medical treatment was 5 % permethrin lotion for three days. Use of biosafety standards is recommended for patient care, especially when patients are admitted to the hospital with skin lesions. The implemented measures must be considered to prevent and control this type of outbreak.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"57-62"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47584381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.24265/horizmed.2019.v19n4.05
M. T. Rivera-Encinas, J. Huarcaya-Victoria
espanolObjetivo: Establecer la validez y fiabilidad de la Escala de actitud religiosa (EAR) en una muestra de estudiantes de medicina de una universidad privada del Peru. Materiales y metodos: Estudio observacional y transversal. Se aplico la EAR a una muestra de 687 estudiantes de medicina para valorar las propiedades psicometricas. Resultados: El puntaje medio de la EAR fue de 62,97 ± 15,74. Destaca la diferencia en variables genero (p=0,011), ano de estudio (p=0,035), y religion (p=0,000). Se encontraron 3 factores con autovalores de 9,34, 1,53 y 1,02 respectivamente. Cada uno aporta el 35,22 %, 17,68 % y 17,09 % de la varianza total explicada en la rotacion, que es, en conjunto, el 70 % de la varianza total. La EAR tiene una consistencia interna aceptable (alfa de Cronbach=0,94), pero en su version de 14 items aumenta a 0,951. Conclusiones: Se puede inferir que la EAR, en su version de 14 items, cuenta con propiedades psicometricas adecuadas para poder evaluar la actitud de la poblacion de estudiantes peruanos de Medicina Humana hacia la religion. EnglishObjective: To establish the validity and reliability of the Religious Attitude Scale (RAS) in a sample of medical students from a Peruvian private university. Materials and methods: An observational and cross-sectional study was conducted. The RAS was administered to a sample of 687 medical students to assess the psychometric properties. Results: The mean score of the RAS was 62.97 ± 15.74. Differences in gender (p = 0.011), academic year (p = 0.035) and religion (p = 0.000) stand out. Three factors with eigenvalues of 9.34, 1.53 and 1.02 were found, each contributing 35.22 %, 17.68 % and 17.09 % of the total variance explained in t he rotation, which accounts for 70 % of the total variance. The RAS has an acceptable internal consistency (Cronbach's alpha = 0.94); however, in its 14-item version it increases to 0.951. Conclusions: It can be concluded that the RAS, in its 14-item version, has adequate psychometric properties to evaluate the attitude of the population of Peruvian students of human medicine toward religion.
{"title":"Escala de actitud religiosa en estudiantes de Medicina Humana","authors":"M. T. Rivera-Encinas, J. Huarcaya-Victoria","doi":"10.24265/horizmed.2019.v19n4.05","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.05","url":null,"abstract":"espanolObjetivo: Establecer la validez y fiabilidad de la Escala de actitud religiosa (EAR) en una muestra de estudiantes de medicina de una universidad privada del Peru. Materiales y metodos: Estudio observacional y transversal. Se aplico la EAR a una muestra de 687 estudiantes de medicina para valorar las propiedades psicometricas. Resultados: El puntaje medio de la EAR fue de 62,97 ± 15,74. Destaca la diferencia en variables genero (p=0,011), ano de estudio (p=0,035), y religion (p=0,000). Se encontraron 3 factores con autovalores de 9,34, 1,53 y 1,02 respectivamente. Cada uno aporta el 35,22 %, 17,68 % y 17,09 % de la varianza total explicada en la rotacion, que es, en conjunto, el 70 % de la varianza total. La EAR tiene una consistencia interna aceptable (alfa de Cronbach=0,94), pero en su version de 14 items aumenta a 0,951. Conclusiones: Se puede inferir que la EAR, en su version de 14 items, cuenta con propiedades psicometricas adecuadas para poder evaluar la actitud de la poblacion de estudiantes peruanos de Medicina Humana hacia la religion. EnglishObjective: To establish the validity and reliability of the Religious Attitude Scale (RAS) in a sample of medical students from a Peruvian private university. Materials and methods: An observational and cross-sectional study was conducted. The RAS was administered to a sample of 687 medical students to assess the psychometric properties. Results: The mean score of the RAS was 62.97 ± 15.74. Differences in gender (p = 0.011), academic year (p = 0.035) and religion (p = 0.000) stand out. Three factors with eigenvalues of 9.34, 1.53 and 1.02 were found, each contributing 35.22 %, 17.68 % and 17.09 % of the total variance explained in t he rotation, which accounts for 70 % of the total variance. The RAS has an acceptable internal consistency (Cronbach's alpha = 0.94); however, in its 14-item version it increases to 0.951. Conclusions: It can be concluded that the RAS, in its 14-item version, has adequate psychometric properties to evaluate the attitude of the population of Peruvian students of human medicine toward religion.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"31-40"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43038927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.24265/horizmed.2019.v19n4.03
Enrique Eduardo Sanchez-Castro, Renato LaTorre-Ramírez, Carlos Patricio Padilla Rojas, Frank Lizaraso-Soto, J. Calderón Ticona, José Enrique Solís Villanueva, Wilser Andrés García-Quispes, Mónica Yolanda Paredes Anaya
espanolObjetivo: Analizar la asociacion de los polimorfismos de un solo nucleotido (SNP) rs941798 y rs914458 del gen PTPN1 con la diabetes tipo 2 en familias peruanas de Lima. Materiales y metodos: Veintitres trios familiares fueron captados en el Hospital Nacional Arzobispo Loayza. Se extrajeron muestras de sangre periferica para obtener el ADN y luego las frecuencias alelicas y genotipicas de los SNP. La genotipificacion de los SNP se realizo mediante secuenciacion. El analisis de asociacion basado en familias entre los SNP y la diabetes tipo 2 se realizo con el programa FBAT. Resultados: Se observaron los 3 genotipos posibles para cada SNP, rs941798 (A>G) y rs914458 (G>C). Las pruebas de asociacion basada en familias a nivel alelico mostraron al alelo A del SNP rs941798 asociado a la diabetes tipo 2 (p = 0.023) bajo uno de los modelos evaluados; no obstante, tras la correccion de Bonferroni para comparaciones multiples, esta asociacion se perdio. No se evidencio asociacion entre los SNP y la enfermedad en ningun nivel (alelico, genotipico o haplotipico). Conclusiones: No se encontraron evidencias de asociacion significativa entre los SNP rs941798 y rs914458 del gen PTPN1 con la DT2 en familias peruanas de Lima, en ninguno de los niveles estudiados (alelico, genotipico y haplotipico). EnglishObjective: To analyze the association that PTPN1 gene single nucleotide polymorphisms (SNPs) rs941798 and rs914458 have with type 2 diabetes in Peruvian families from Lima. Materials and methods: Twenty-three (23) families consisting of three members each were recruited at the Hospital Nacional Arzobispo Loayza. Peripheral blood samples were collected to obtain the DNA, and then the allele and genotype frequencies of the SNPs. SNP genotyping was performed using the sequencing method. The family-based analysis of the association between SNPs and type 2 diabetes was conducted using the family-based association test (FBAT) program. Results: Three (3) possible genotypes were observed for each SNP, i.e. rs941798 (A>G) and rs914458 (G>C). In one of the assessed models, the family-based association tests showed at the allele level that allele A of SNP rs941798 is associated with type 2 diabetes (p = 0.023). However, after using the Bonferroni correction for multiple comparisons, this association was lost. No association was demonstrated between the SNPs and the disease at any level (allele, genotype or haplotype). Conclusions: No evidence of significant association was found between PTPN1 gene SNPs rs941798 and rs914458 and type 2 diabetes at the studied levels (allele, genotype or haplotype) in Peruvian families from Lima.
西班牙lobjetivo:分析秘鲁利马家庭PTPN1基因rs941798和rs914458单核苷酸多态性与2型糖尿病的关系。本研究的目的是确定在墨西哥洛伊扎大主教国立医院(Loayza大主教国立医院)的23名家庭成员。采集周围血液样本进行dna分析，然后测定等位基因和SNP基因型频率。通过测序对SNP进行基因分型。使用FBAT程序对SNP和2型糖尿病进行基于家庭的关联分析。结果:每个SNP有3种可能的基因型，rs941798 (A>G)和rs914458 (G>C)。基于等位基因水平的家族关联试验显示SNP rs941798的a等位基因与2型糖尿病相关(p = 0.023);然而，在Bonferroni对多次比较进行修正后，这种关联消失了。在任何水平上(等位基因、基因型或单倍型)均未发现SNP与疾病之间的关联。结论:在秘鲁利马家庭中，PTPN1基因的SNP rs941798和rs914458与tt2基因的任何水平(等位基因、基因型和单倍型)均无显著相关性。目的:分析来自利马的秘鲁家庭的PTPN1基因单核苷酸多态性(SNPs) rs941798和rs914458与2型糖尿病的关系。材料和方法:在洛伊扎大主教国立医院招募了23个家庭，每个家庭有3名成员。Peripheral血液samples民工指导to obtain the DNA,然后allele和genotype frequencies of the SNPs。= =地理= =根据美国人口普查，这个县的总面积为，其中土地和(0.964平方公里)水。使用以家庭为基础的关联试验(FBAT)方案对SNPs与2型糖尿病之间的关联进行了基于家庭的分析。结果:在每个SNP中观察到3个可能的基因型，即rs941798 (A>G)和rs914458 (G>C)。在一种评估模型中，基于家庭的关联试验显示，SNP rs941798的水平与2型糖尿病有关(p = 0.023)。然而，在使用Bonferroni修正进行多次比较后，这种关联就消失了。在任何水平(allele、基因型或单倍型)，SNPs与疾病之间没有关联。结论:在来自利马的秘鲁家庭中，没有证据表明PTPN1基因SNPs rs941798和rs914458与研究水平(allele、基因型或单倍型)的2型糖尿病之间存在显著关联。
{"title":"Análisis de asociación entre polimorfismos (rs941798 y rs914458) del gen PTPN1 y diabetes tipo 2 en familias de Lima-Perú","authors":"Enrique Eduardo Sanchez-Castro, Renato LaTorre-Ramírez, Carlos Patricio Padilla Rojas, Frank Lizaraso-Soto, J. Calderón Ticona, José Enrique Solís Villanueva, Wilser Andrés García-Quispes, Mónica Yolanda Paredes Anaya","doi":"10.24265/horizmed.2019.v19n4.03","DOIUrl":"https://doi.org/10.24265/horizmed.2019.v19n4.03","url":null,"abstract":"espanolObjetivo: Analizar la asociacion de los polimorfismos de un solo nucleotido (SNP) rs941798 y rs914458 del gen PTPN1 con la diabetes tipo 2 en familias peruanas de Lima. Materiales y metodos: Veintitres trios familiares fueron captados en el Hospital Nacional Arzobispo Loayza. Se extrajeron muestras de sangre periferica para obtener el ADN y luego las frecuencias alelicas y genotipicas de los SNP. La genotipificacion de los SNP se realizo mediante secuenciacion. El analisis de asociacion basado en familias entre los SNP y la diabetes tipo 2 se realizo con el programa FBAT. Resultados: Se observaron los 3 genotipos posibles para cada SNP, rs941798 (A>G) y rs914458 (G>C). Las pruebas de asociacion basada en familias a nivel alelico mostraron al alelo A del SNP rs941798 asociado a la diabetes tipo 2 (p = 0.023) bajo uno de los modelos evaluados; no obstante, tras la correccion de Bonferroni para comparaciones multiples, esta asociacion se perdio. No se evidencio asociacion entre los SNP y la enfermedad en ningun nivel (alelico, genotipico o haplotipico). Conclusiones: No se encontraron evidencias de asociacion significativa entre los SNP rs941798 y rs914458 del gen PTPN1 con la DT2 en familias peruanas de Lima, en ninguno de los niveles estudiados (alelico, genotipico y haplotipico). EnglishObjective: To analyze the association that PTPN1 gene single nucleotide polymorphisms (SNPs) rs941798 and rs914458 have with type 2 diabetes in Peruvian families from Lima. Materials and methods: Twenty-three (23) families consisting of three members each were recruited at the Hospital Nacional Arzobispo Loayza. Peripheral blood samples were collected to obtain the DNA, and then the allele and genotype frequencies of the SNPs. SNP genotyping was performed using the sequencing method. The family-based analysis of the association between SNPs and type 2 diabetes was conducted using the family-based association test (FBAT) program. Results: Three (3) possible genotypes were observed for each SNP, i.e. rs941798 (A>G) and rs914458 (G>C). In one of the assessed models, the family-based association tests showed at the allele level that allele A of SNP rs941798 is associated with type 2 diabetes (p = 0.023). However, after using the Bonferroni correction for multiple comparisons, this association was lost. No association was demonstrated between the SNPs and the disease at any level (allele, genotype or haplotype). Conclusions: No evidence of significant association was found between PTPN1 gene SNPs rs941798 and rs914458 and type 2 diabetes at the studied levels (allele, genotype or haplotype) in Peruvian families from Lima.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"19 1","pages":"14-19"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48583380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.24265/horizmed.2018.v18n4.06
Frank Dennys Tamariz Chavarria
{"title":"Nivel de conocimiento y práctica de medidas de bioseguridad: Hospital San José, 2016","authors":"Frank Dennys Tamariz Chavarria","doi":"10.24265/horizmed.2018.v18n4.06","DOIUrl":"https://doi.org/10.24265/horizmed.2018.v18n4.06","url":null,"abstract":"","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68832670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.24265/horizmed.2018.v18n4.03
Gerly Edson Guzmán Calderón
{"title":"Resangrado de la úlcera péptica en pacientes con y sin enfermedad renal crónica","authors":"Gerly Edson Guzmán Calderón","doi":"10.24265/horizmed.2018.v18n4.03","DOIUrl":"https://doi.org/10.24265/horizmed.2018.v18n4.03","url":null,"abstract":"","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.24265/horizmed.2018.v18n4.03","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68832618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.24265/horizmed.2018.v18n1.03
Carlos René Rodríguez Garcés, Geraldo Bladimir Padilla Fuentes
Objetivo: modelar estadisticamente los niveles de riesgo al absentismo preventivo de Papanicolaou que presentan las mujeres chilenas segun variables sociodemograficas. Materiales y metodos: Encuesta de Caracterizacion Socioeconomica Nacional. Muestra de 73 324 casos segun alcances CASEN. Modelamiento segun Arboles de Decision para establecer perfiles de la mujer que se abstiene y analizar luego los motivos que esgrimen para tal decision. Resultados: el riesgo de abstencion al examen varia considerablemente en razon de tomar otros examenes de prevencion de cancer (no tomar mamografia), encontrarse en determinada etapa de la adultez (tardia) y tener algun tipo de vinculo conyugal; al tradicional deficit de informacion y acceso le sustituyen los motivos personales como excusa frecuente para no considerar el Papanicolaou como accion recurrente. Asi, la percepcion de poca utilidad y la falta de tiempo configuran el comportamiento de abstencion como una decision en lugar de una imposibilidad. Conclusiones: Tales resultados problematizan las caracteristicas de la mujer contemporanea y sus comportamientos, ademas de plantear la necesidad de considerar la intervencion socioeducativa como requisito para generar actitudes favorables hacia la prevencion; factores que debiera considerar la politica social de prevencion del cancer en general y de cervix en particular.
{"title":"Cáncer de cérvix y autorresponsabilidad: perfilando el riesgo de abstención al Papanicolaou entre las mujeres chilenas mediante Árboles de Decisión","authors":"Carlos René Rodríguez Garcés, Geraldo Bladimir Padilla Fuentes","doi":"10.24265/horizmed.2018.v18n1.03","DOIUrl":"https://doi.org/10.24265/horizmed.2018.v18n1.03","url":null,"abstract":"Objetivo: modelar estadisticamente los niveles de riesgo al absentismo preventivo de Papanicolaou que presentan las mujeres chilenas segun variables sociodemograficas. Materiales y metodos: Encuesta de Caracterizacion Socioeconomica Nacional. Muestra de 73 324 casos segun alcances CASEN. Modelamiento segun Arboles de Decision para establecer perfiles de la mujer que se abstiene y analizar luego los motivos que esgrimen para tal decision. Resultados: el riesgo de abstencion al examen varia considerablemente en razon de tomar otros examenes de prevencion de cancer (no tomar mamografia), encontrarse en determinada etapa de la adultez (tardia) y tener algun tipo de vinculo conyugal; al tradicional deficit de informacion y acceso le sustituyen los motivos personales como excusa frecuente para no considerar el Papanicolaou como accion recurrente. Asi, la percepcion de poca utilidad y la falta de tiempo configuran el comportamiento de abstencion como una decision en lugar de una imposibilidad. Conclusiones: Tales resultados problematizan las caracteristicas de la mujer contemporanea y sus comportamientos, ademas de plantear la necesidad de considerar la intervencion socioeducativa como requisito para generar actitudes favorables hacia la prevencion; factores que debiera considerar la politica social de prevencion del cancer en general y de cervix en particular.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.24265/horizmed.2018.v18n1.03","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68832636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.24265/horizmed.2018.v18n3.12
Elert Zapata Aguilar, Víctor Hugo Calderón Arenas, Lizeth Y. Cabanillas Burgos
El sindrome de Jeune es una displasia esqueletica de baja frecuencia, de transmision autosomica recesiva y potencialmente mortal. Clinicamente tiene una amplia variedad de manifestaciones; En forma clasica se presenta con un torax estrecho, hipoplasia pulmonar secundaria, braquimelia, anormalidades pelvicas, cardiacas y renales. El compromiso pulmonar es variable y puede ser letal. El diagnostico puede sospecharse prenatalmente con el estudio ultrasonografico al medir la circunferencia toracica y de la jaula costal, estudiar su relacion entre si y con la circunferencia abdominal. En este articulo se describe el caso clinico de una paciente de 3 meses de edad, con forma de presentacion leve y diagnostico posnatal.
{"title":"Síndrome de Jeune en un paciente pediátrico: Reporte de caso clínico","authors":"Elert Zapata Aguilar, Víctor Hugo Calderón Arenas, Lizeth Y. Cabanillas Burgos","doi":"10.24265/horizmed.2018.v18n3.12","DOIUrl":"https://doi.org/10.24265/horizmed.2018.v18n3.12","url":null,"abstract":"El sindrome de Jeune es una displasia esqueletica de baja frecuencia, de transmision autosomica recesiva y potencialmente mortal. Clinicamente tiene una amplia variedad de manifestaciones; En forma clasica se presenta con un torax estrecho, hipoplasia pulmonar secundaria, braquimelia, anormalidades pelvicas, cardiacas y renales. El compromiso pulmonar es variable y puede ser letal. El diagnostico puede sospecharse prenatalmente con el estudio ultrasonografico al medir la circunferencia toracica y de la jaula costal, estudiar su relacion entre si y con la circunferencia abdominal. En este articulo se describe el caso clinico de una paciente de 3 meses de edad, con forma de presentacion leve y diagnostico posnatal.","PeriodicalId":30557,"journal":{"name":"Horizonte Medico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68832603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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