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Diversity of Ethiopian Fenugreek (Trigonella foenum-graecum L.) Accessions Based on Agromorphological Traits 埃塞俄比亚胡芦巴(Trigonella foenum-graecum L.)多样性基于农型性状的材料
Q3 Agricultural and Biological Sciences Pub Date : 2022-11-03 DOI: 10.1155/2022/4844828
Rameto Roba, W. Mohammed, Morgan L. Ruelle, T. Tana, Tariku Simion Dojamo
Information on the diversity of landraces is necessary to improve crops through selection or hybridization. This study was conducted to show the diversity of fenugreek landraces and associated traits. A total of 160 accessions including one local and four improved standard checks were evaluated in an augmented block design at the Haramaya University research site in 2016. Data collected include days to flowering, days to maturity, seed yield (kg/ha), thousand seed weight (g), the number of primary branches, plant height at flowering (cm), the number of pods per plant, the number of secondary branches, average pod length (cm), the number of seeds per pod, and seed yield per plant (g/plant) of quantitative traits. The analysis of variance revealed the existence of significant differences between accessions of all parameters. Genotypic and phenotypic coefficients of variation departed from 5.95–56.91% and 6.47–58.88%. Heritability in the broad sense and expected genetic gain as percent mean varied from 60.9 to 96.1% and from 2.5 to 70.3%. The seed yield per plant, the number of secondary branches, and the number of pod per plant had positive direct effects on the yield at both genotypic and phenotypic levels and the number of primary branches, and the average pod length via seed yield per plant, while the number of seeds per plant through the number of primary branches and the number of pods per plant had a positive indirect effect on the yield at the genotypic level, suggesting that these traits could be considered for indirect selection of genotypes for yield. The genetic distances of genotypes measured by Euclidean distance ranged from 0.07 to 10.6, and the dendrogram was constructed by using the unweighted pair group method using arithmetic mean. The presence of variability among fenugreek accessions suggested possibilities to improve the crop through the crossing of distant genotypes. This was an excellent opportunity to contribute to farmers’ food security and livelihoods by bringing about the improvement of fenugreek.
关于地方品种多样性的信息对于通过选择或杂交来改进作物是必要的。本研究旨在揭示胡芦巴地方品种及其相关性状的多样性。2016年,在原屋大学研究基地的一个增强块设计中,共评估了160个条目,其中包括一个本地和四个改进的标准检查。收集的数据包括数量性状的开花天数、成熟天数、种子产量(kg/ha)、千粒重(g)、一次枝数、开花株高(cm)、单株荚果数、次枝数、平均荚果长(cm)、每荚种子数和单株种子产量(g/株)。方差分析表明,各参数间存在显著差异。基因型变异系数为5.95 ~ 56.91%,表型变异系数为6.47 ~ 58.88%。广义遗传率和平均预期遗传增益百分比分别为60.9 ~ 96.1%和2.5 ~ 70.3%。单株种子产量、次枝数和单株荚果数在基因型和表型水平上对产量均有直接正向影响,单株一次枝数和平均荚果长通过单株种子产量均有正向影响,单株种子数通过一次枝数和单株荚果数对基因型水平上的产量均有间接正向影响。提示这些性状可作为产量基因型的间接选择。欧几里得距离测量的基因型遗传距离在0.07 ~ 10.6之间,采用算术平均的非加权对群法构建树形图。胡芦巴品种间变异的存在表明通过远缘基因型杂交改良作物的可能性。这是一个极好的机会,通过改善胡芦巴,为农民的粮食安全和生计做出贡献。
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引用次数: 0
Genetic Variability Analysis and Association of Traits in Common Bean (Phaseolus vulgaris L.) Landraces Collected from Ethiopia at Jimma 菜豆(Phaseolus vulgaris L.)性状遗传变异分析及关联分析在吉马从埃塞俄比亚收集的长马
Q3 Agricultural and Biological Sciences Pub Date : 2022-11-03 DOI: 10.1155/2022/4400711
Eyuel Mesera, Garome Shifaraw, S. Alamerew, Birhanu Amsalu
This study was conducted on one hundred common bean landraces at the Jimma Agricultural Research Center, Melko, with the objective of assessing genetic variability and association of traits in common bean landraces collected from different parts of Ethiopia. The experiment was laid out in a simple lattice design with two replications. Analysis of variance showed significant differences among genotypes for all traits. This highly significant difference indicates the existence of large variability among genotypes. High phenotypic coefficients of variation and genotypic coefficients of variation were obtained for plant height (19.43, 11.73), pod length (11.27, 10.69), and 100-seed weight (15.42, 12.74). High heritability in the broad sense was found for days to 50% flowering (66.98), days to 90% maturity (87.43), pod length (90.03), pod width (78.23), harvest index (98.67), and 100-seed weight (68.31). High genetic advance as a percentage of mean with high heritability was obtained for pod length, pod width, harvest index, and hundred seed weight. Grain yield had a positive and significant association with pod length (rp = 0.153 ∗ , rg = 0.282 ∗ ∗ ) and 100-seed weight (rp = 0.294 ∗ ∗ , rg = 0.492 ∗ ∗ ). Hundred seed weight exerted the highest positive direct effect (0.294) on grain yield at genotypic level. The D2 classified landraces into 7 clusters and one solitary, which makes them moderately divergent. The highest inter-cluster distance was observed between clusters VII and IV. The first five principal components with eigenvalues greater than one altogether explained about 79.56% of the total variation. In conclusion, the top high-yielding landraces, namely, P#1247, P#1092, P#1077, P#861, P#990, P#763, P#58, and P#857, should be included in the next breeding program. 100-seed weight had the highest direct effect and a positive significant association with grain yield. Thus, it should be considered as the selection criteria for further common bean yield improvement. However, the current result is merely indicative and cannot be used to draw definite conclusions. Therefore, the experiment should be replicated in different locations and seasons for greater consistency.
本研究在Melko的Jimma农业研究中心对100个普通豆地方品种进行了研究,目的是评估从埃塞俄比亚不同地区收集的普通豆地方品种的遗传变异和性状关联。实验以简单的格子设计进行,有两个重复。方差分析表明,各性状基因型间差异显著。这种高度显著的差异表明基因型之间存在很大的变异性。株高(19.43,11.73)、荚果长(11.27,10.69)和百粒重(15.42,12.74)的表型变异系数和基因型变异系数均较高。开花至50%天数(66.98)、成熟至90%天数(87.43)、荚果长度(90.03)、荚果宽度(78.23)、收获指数(98.67)和百粒重(68.31)具有较高的广义遗传力。在荚果长、荚果宽、收获指数和百粒重等性状上均取得较高的遗传优势,遗传力高于平均值。籽粒产量与荚果长(rp = 0.153∗,rg = 0.282∗)和百粒重(rp = 0.294∗,rg = 0.492∗)呈显著正相关。在基因型水平上,百粒重对籽粒产量的直接正向影响最大(0.294)。D2将地方小种分为7个集群和1个孤种,这使得它们有适度的分化。聚类VII和聚类IV的聚类间距离最大。特征值大于1的前5个主成分解释了总变异的79.56%。综上所述,高产地方品种p# 1247、p# 1092、p# 1077、p# 861、p# 990、p# 763、p# 58和p# 857应纳入下一步育种计划。百粒重对籽粒产量的直接影响最大,且与籽粒产量呈显著正相关。因此,应将其作为进一步提高普通豆产量的选择标准。然而,目前的结果仅仅是指示性的,不能用来得出明确的结论。因此,应该在不同的地点和季节重复实验,以获得更大的一致性。
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引用次数: 1
Tea (Camellia sinensis) Production, Opportunities, Challenges, and Future Prospects in Ethiopia 埃塞俄比亚茶(Camellia sinensis)生产、机遇、挑战和未来前景
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-30 DOI: 10.1155/2022/1942666
Nagassa Dechassa, Misgana Merga
Tea is one of the most commonly consumed stimulants in the world. It is cultivated commercially at Wushwush, Gumero, and Chewaka tea estate plantations, in Ethiopia. Over 150,000 hectares of Ethiopian land are available for the production of high-quality tea, but only 3,099 hectares are currently under cultivation. In Ethiopia, tea clones Mlk-1, Mlk-2, 11/56, S-15/10, FNF, 11/4, 6/8, L6, B9, Chai, BB-35, and SR-18 are commercially grown. Ethiopia’s favorable environmental conditions for high-quality tea production increased the availability of tea throughout the year, and the availability of a sufficient labor force is a significant opportunity for tea companies. However, tea production is still infant because it needs huge capital to establish the tea plantations and production, the pest problems, limitations of improved technologies like cultivars, and lack of tea genetic resources. Besides, there is a limitation of comprehensive research to develop innovative technologies in the case of tea agronomic, breeding, and pest management for Ethiopian conditions. Thus, the government should invite investors and facilitate necessary things for the investors to increase the tea industry in the country. The tea research should be strengthened for the development and implementation of the appropriate technologies.
茶是世界上最常用的兴奋剂之一。它在埃塞俄比亚的Wushwush, Gumero和Chewaka茶园种植园进行商业种植。埃塞俄比亚有超过15万公顷的土地可用于生产高质量的茶叶,但目前只有3099公顷的土地正在种植。在埃塞俄比亚,茶叶无品系Mlk-1、Mlk-2、11/56、S-15/10、FNF、11/4、6/8、L6、B9、Chai、BB-35和SR-18已被商业化种植。埃塞俄比亚有利的环境条件有利于高质量的茶叶生产,增加了全年茶叶的供应,充足的劳动力是茶叶公司的重要机会。然而,茶叶生产仍然处于初级阶段,因为建立茶园和生产需要大量资金,害虫问题,改良技术如品种的局限性,以及缺乏茶叶遗传资源。此外,在埃塞俄比亚的茶叶农艺、育种和病虫害管理方面,综合研究开发创新技术也存在局限性。因此,政府应该邀请投资者,并为投资者提供必要的便利,以增加该国的茶业。应加强对茶叶的研究,以开发和实施适当的技术。
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引用次数: 1
Phenotypic Characterization and Reproductive Performance of Blackhead Somali Sheep in Fafen Zone of Somali Regional State, Ethiopia 埃塞俄比亚索马里地区法芬地区黑头索马里羊的表型特征和繁殖性能
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-27 DOI: 10.1155/2022/4865941
Abdimalik Hussein Mohamed, B. Gebrekidan, Niraj Kumar, Haben Fesseha Gebremeskel, Mesfin Mathewos Abose
Objective. The study aimed to characterize the reproductive performances and physical characteristics of Blackhead Somali indigenous sheep breeds. Methods. The data from 460 sheep were used for the determination of morphometric characterization while 110 male sheep and 150 females were used to characterize the reproductive performance of Blackhead Somali sheep breeds. Results. Accordingly, about 86% of sheep have a white body with a blackhead as the dominant coat color and about 81% of sheep have a straight facial head profile. Dewlap was present in 92.7% (91.8% for males and 92.7% for females). The overall average body weight, body length, height at wither, height at the rump, heart girth, chest depth, pelvic width, and tail circumference differs significantly ( p < 0.05 ) among males and females with the advancement of age. The average age at first service, age at first lambing, lambing interval, and life lamb crop in ewe were 13.75 ± 2.44, 19.12 ± 2.41, 9.66 ± 1.56, and 6.18 ± 2.01, respectively. The average reproductive life span of ewe and ram was 6.04 ± 1.43 and 6.63 ± 1.49 years, respectively. On average Blackhead Somali ewe delivers 13.1 ± 4.3 lambs in her lifetime. The twinning and triplet rate were recorded to be 12.30% and 1.90%, respectively. Conclusion. In conclusion, shorter lambing intervals, higher adult body weight, and frequent twinning are the most desirable features which may also be used as selection criteria. It is recommended to do further detailed analyses to determine the genetic variation between and within these small populations to develop an effective conservation and utilization program.
目标。本研究旨在描述索马里黑头土着羊品种的繁殖性能和身体特征。方法。460只羊的数据用于形态计量学表征,110只公羊和150只母羊用于表征黑头索马里羊品种的繁殖性能。结果。因此,约86%的羊具有白色的身体,黑头是主要的被毛颜色,约81%的羊具有直的面部头部轮廓。Dewlap患者占92.7%(男性91.8%,女性92.7%)。总体平均体重、体长、腰高、臀高、心围、胸深、盆宽、尾围随着年龄的增长,男女差异有统计学意义(p < 0.05)。母羊平均初产龄、初产龄、产羔间隔和终身产羔数分别为13.75±2.44、19.12±2.41、9.66±1.56和6.18±2.01。母羊和公羊的平均生殖寿命分别为6.04±1.43年和6.63±1.49年。黑头索马里母羊一生平均产羔13.1±4.3只。双胞胎和三胞胎的比例分别为12.30%和1.90%。结论。综上所述,较短的产羔间隔,较高的成虫体重和频繁的双胞胎是最理想的特征,也可以作为选择标准。建议进一步详细分析这些小种群之间和内部的遗传变异,以制定有效的保护和利用方案。
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引用次数: 0
Establishment of a Highly Efficient Corn Stock-Degrading Microbial Consortium and Its Degradation Effect 高效玉米秸秆降解菌群的建立及其降解效果
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-26 DOI: 10.1155/2022/8034553
Erhong Zhang, Meng Wang, Xiaoliang Pan, Xinfeng Wang
Corn stalks are not easily degradable and thus have a low utilization rate. In this paper, a highly efficient corn stalk-degrading microbial consortium, designated as SDP, was established using the strains Z22 (Bacillus subtilis), Z15 (Bacillus megaterium), and Z08 (Aspergillus tubingensis). Moreover, the fermentation conditions for enzyme production by SDP were optimized through orthogonal experiments. The Van Soest method was used to determine the cellulose, hemicellulose, and lignin contents in the degraded corn stalks using the SDP consortium to evaluate its degrading effect. Scanning electron microscopy was used to analyze the micromorphological structure changes of the stalks to evaluate the degradation effects of SDP on corn stalks. The carboxymethyl cellulase activity of SDP reached 6.37 U/mL. Moreover, the enzyme production effect was optimal when the culture conditions were as follows: temperature, 30°C; time, 5 d; nitrogen source, NH4NO3; and initial pH, 7.0. The SDP consortium showed an improved ability to degrade corn stalks. The cellulose, hemicellulose, and lignin contents were reduced by 32.15%, 23.87%, and 7.98%, respectively, after culturing for 20 d. This study provides insights to guide further research and development of consortia for efficient corn stalk degradation.
玉米秸秆不易降解,利用率低。以枯草芽孢杆菌(Bacillus subtilis) Z22、巨芽孢杆菌(Bacillus megaterium) Z15和塔宾曲霉(Aspergillus tubingensis) Z08为菌株,建立了玉米秸秆高效降解菌群SDP。通过正交试验对SDP产酶的发酵条件进行了优化。采用Van Soest法测定降解后玉米秸秆中纤维素、半纤维素和木质素的含量,评价其降解效果。采用扫描电子显微镜对秸秆的微观形态结构变化进行分析,评价SDP对玉米秸秆的降解效果。SDP羧甲基纤维素酶活性达到6.37 U/mL。当培养条件为温度30℃时,产酶效果最佳;时间,5天;氮源NH4NO3;初始pH 7.0。SDP菌群对玉米秸秆的降解能力有所提高。培养20 d后,纤维素、半纤维素和木质素含量分别降低32.15%、23.87%和7.98%。该研究为指导玉米秸秆高效降解菌群的进一步研究和开发提供了参考。
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引用次数: 0
Growth Promotion of Rice (Oryza sativa L.) Seedlings Using Plant Growth-Promoting Rhizobacteria (PGPR) Isolated from Northwest Ethiopia 水稻的生长促进作用埃塞俄比亚西北部植物促生根瘤菌(PGPR)育苗研究
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-26 DOI: 10.1155/2022/1710737
Z. T. Awlachew, Gebeyehu Yibeltie Mengistie
Plant growth-promoting rhizobacteria (PGPR) are beneficial soil microorganisms that colonize plant roots and enhance plant growth by a wide variety of mechanisms. In this work, five Bacillus and two Cyanobacteria isolates were successfully isolated and characterized. A pot experiment was conducted to evaluate the effect of PGPR on the growth of three cultivars of rice seedlings. Pots were laid down in a complete random design and 100 ml of spore and Cyanobacteria suspension were poured on the soil surface surrounding each seedling. After 45 days, the seedlings were uprooted and shoot and root parameters were recorded. All the Bacillus and Cyanobacteria isolates showed positive effects on the growth of rice seedlings as compared to control; however, their effectiveness varies from isolate to isolate and also from cultivar to cultivar. Bacterial isolates B3 and B5 showed the highest mean value and statistically significant difference ( P < 0.05 ) in most of the root and shoot parameters of cultivars Jegna and Getachew, respectively, as compared to other bacterial isolates. Both cyanobacterial isolates showed the highest and statistically significant difference ( P < 0.01 ) in almost all the above ground and underground growth parameters compared to other bacterial isolates in all the three cultivars of rice. Similarly, C2 and C1 recorded the highest growth promotion efficacy of shoot and root length (50.07% and 78.27%) on Edget and Getachew cultivars, respectively. Hence, the present study suggests that the use of PGPR isolates such as B3, B5, C1, and C2 as inoculant biofertilizers might be beneficial for rice cultivation as they enhanced the growth of rice seedlings.
促进植物生长的根瘤菌(PGPR)是一种有益的土壤微生物,它们定殖于植物根系并通过多种机制促进植物生长。本研究成功分离了5株芽孢杆菌和2株蓝藻,并对其进行了表征。通过盆栽试验,研究了PGPR对3个水稻品种幼苗生长的影响。以完全随机设计的方式放置花盆,将100毫升孢子和蓝藻悬浮液倒在每个幼苗周围的土壤表面。45 d后,将幼苗连根拔起,记录茎部和根系参数。与对照相比,芽孢杆菌和蓝藻菌株对水稻幼苗的生长均有积极影响;然而,它们的效力因分离物和品种而异。菌株B3和B5在Jegna和Getachew的大部分根和茎参数上的平均值最高,与其他菌株相比差异有统计学意义(P < 0.05)。蓝藻菌株在地上和地下几乎所有生长参数上与其他菌株相比差异均最高(P < 0.01)。同样,C2和C1在Edget和Getachew品种上对茎长和根长促进效果最高,分别为50.07%和78.27%。因此,本研究表明,使用PGPR分离物B3、B5、C1和C2作为接种生物肥料可能有利于水稻种植,因为它们可以促进水稻幼苗的生长。
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引用次数: 1
A Review on Major Rust Resistance Gene and Amino Acid Changes on Wheat (Triticum aestivum L) 小麦(Triticum aestium L)主要抗锈基因及氨基酸变化研究进展
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-25 DOI: 10.1155/2022/7419326
Bikas Basnet, Philomin Juliana, K. Bhattarai, Umisha Upreti
Wheat ranks first in the production and productivity of staple cereal crops in the world. Several diseases, including Stripe (Puccinia striiformis f. Sp. tritici), Black (Puccinia graminis f. Sp. tritici), and Brown (Puccinia recondita), have a major negative impact on wheat output, with 20 to 80% loss annually. Growing rust-resistant varieties is the most durable, cost-effective, and environmentally friendly way to combat rust pathogens. In the present review, we provide updated information on all black stem rust, yellow leaf rust, and brown leaf rust resistance genes including chromosomal position, those derived from different sources, nature of resistance type, and amino acid changes done by this gene against rust pathogen. This study summarized the 68 black stem rust, 101 leaf rust, and 108 stripe rust resistance genes from diverse cultivars of wheat and wheat primary and secondary gene pools. This review will be valuable to wheat breeders in cloning rust-resistant genes and developing leaf as well as stem rust-resistant wheat cultivars using gene pyramiding as well as frequency multiplication through introgression of the gene of interest for disease-free, sustainable grain production of wheat. The success of pyramiding genes from other sources to bread wheat depends on the nature of germplasm, the gap between flanking marker and targeted genes, the selection of genotypes in each generation, large number of gentoyes large genotype-environment interaction, etc., which is the future area of study.
小麦在世界主要谷类作物的产量和生产力中居首位。小麦条锈病(striiformis f. Sp. tritici)、黑病(Puccinia graminis f. Sp. tritici)和褐病(Puccinia recondita)等几种病害对小麦产量产生重大负面影响,每年损失20%至80%。种植抗锈病品种是对抗锈病病原体最持久、最经济、最环保的方法。本文综述了黑茎锈病、黄叶锈病和褐叶锈病抗性基因的最新研究进展,包括染色体位置、不同来源的基因、抗性类型的性质以及该基因对病原菌的氨基酸变化。本研究总结了来自不同小麦品种和小麦主次基因库的68个黑茎锈病抗性基因、101个叶锈病抗性基因和108个条锈病抗性基因。本文的研究成果对小麦育种人员克隆抗锈病基因,利用基因金字塔和基因基因的遗传育种技术培育抗锈病小麦品种,实现小麦的无害化、可持续生产具有重要的参考价值。将其他来源的基因金字塔化到面包小麦上的成功取决于种质的性质、侧翼标记与靶基因之间的差距、每代基因型的选择、大量的gentoyes、大的基因型与环境的相互作用等,这是未来的研究领域。
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引用次数: 1
Participatory Evaluation of Open Pollinated Maize (Zea mays L.) Varieties for Green Cob Production Under Irrigation in the North Shewa Lowlands, Ethiopia 开放授粉玉米(Zea mays L.)参与式评价埃塞俄比亚北谢瓦低地灌溉条件下生产绿玉米棒的品种
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-19 DOI: 10.1155/2022/1984478
Shumet Chakle, Fekadu Tewolde, D. Mamo
In the experimental area, there is a lack of improved maize varieties for green cob production. The experiment was conducted at Efratana gidim woreda Yimlo Kebele FTC-station in the North Shewa zone during the 2017 and 2018 experimental years under irrigation conditions. The objective of the study was to select adaptive, high-green cob yielders and farmers who preferred open-pollinated maize varieties for the lowland areas of North Shewa. The experiment was laid out in a randomized complete block design with three replications. Six nationally released open-pollinated maize, varieties including local check, were evaluated for two subsequent years. The combined analysis of variance showed highly significant ( p < 0.01 ) differences for days to anthesis, days to silking, and number of cobs ha−1. In each year, farmers participated and selected the top preferred varieties. During the 2017 experimental year, farmers used a number of cobs per plant, bear tip, cob length, uniformity, husk tip coverage, earliness, and stalk strength as selection criteria, and during the 2018 experimental year, farmers also used cob length, bear tip, earliness, lodging tolerance, and biomass as variety selection criteria. Varieties Melkasa-2 and Melkasa-6Q have been selected in both years. Analysis of variance revealed that both farmers preferred varieties that gave a high number of cobs, which is 46,914 and 41,358 cobs ha−1 for varieties Melkasa-2 and Melkasa-6Q, respectively. Based on this result, varieties Melkasa-2 and Melkasa-6Q were recommended for the North Shewa lowlands and similar agroecology.
在试验区,缺乏用于绿穗轴生产的改良玉米品种。该试验于2017年和2018年在北谢瓦地区的Efratana gidim woreda Yimlo Kebele ftc站进行,试验条件为灌溉。该研究的目的是为北谢瓦低地地区选择适应性强、高青穗轴产量的作物和喜欢开放授粉的玉米品种的农民。试验采用完全随机区组设计,设3个重复。六个国家发布的开放授粉玉米品种,包括地方检查,在随后的两年进行了评估。组合方差分析表明,花期、出丝期和穗轴数差异极显著(p < 0.01)。每年,农民都参加并选出最受欢迎的品种。在2017年试验年,农民以每株穗轴数、穗尖、穗轴长度、均匀性、壳尖覆盖率、早熟性和茎秆强度作为选择标准,在2018年试验年,农民还以穗轴长度、穗尖、早熟性、耐倒伏性和生物量作为品种选择标准。品种Melkasa-2和Melkasa-6Q在这两年都被选中。方差分析显示,两名农民都更喜欢穗轴数量多的品种,Melkasa-2和Melkasa-6Q分别为46,914和41,358穗轴ha - 1。在此基础上,推荐品种Melkasa-2和Melkasa-6Q用于北舍瓦低地和类似的农业生态。
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引用次数: 0
Physiological Responses and Tolerance of Halophyte Sesuvium portulacastrum L. to Cesium 盐生植物马齿苋对铯的生理反应及耐受性
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-18 DOI: 10.1155/2022/9863002
G. Nikalje, M. Shrivastava, T. Nikam, P. Suprasanna
Cesium (Cs) is a soil contaminant and toxic to the ecosystem, especially the plant species. In this study, we have assessed the potential of a halophyte Sesuvium portulacastrum for its Cs tolerance and accumulation. Thirty days old S. portulacastrum plants were subjected to different concentrations of Cs (0, 5, 10, 25, 50, and 150 mg·L−1 Cs) using cesium chloride. The biomass and photosynthetic pigments were not affected up to 25 mg·L−1 Cs treatment while a significant decline in pigment levels was observed at higher concentrations. The Cs treatments increased protein content at low concentrations while higher concentrations were inhibitory. Under Cs exposure, significant induction of antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) was observed. The antioxidant enzyme activities were upregulated up to 50 mg·L−1 Cs but decreased significantly at 150 mg·L−1. The accumulation of Cs was dose and tissue-dependent as evidenced by a higher accumulation of Cs in leaves (536.10 μg·g−1) as compared to stem (413.74 μg·g−1) and roots (284.69 μg·g−1). The results suggest that S. portulacastrum is a hyper-accumulator of Cs and could be useful for the phytoremediation of Cs-contaminated soils.
铯(Cs)是一种土壤污染物,对生态系统,特别是植物物种具有毒性。在这项研究中,我们评估了盐生植物马齿苋(Sesuvium portulacastrum)的Cs耐受性和积累潜力。以30 d龄马齿苋植株为研究对象,采用氯化铯处理不同浓度Cs(0、5、10、25、50和150 mg·L−1 Cs)。在25 mg·L−1 Cs处理下,生物量和光合色素未受影响,但在较高浓度下,色素水平显著下降。低浓度Cs处理提高了蛋白质含量,高浓度Cs处理抑制了蛋白质含量。在Cs暴露下,抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和超氧化物歧化酶(SOD)等抗氧化酶显著诱导。当浓度为50 mg·L−1 Cs时,抗氧化酶活性显著升高,而当浓度为150 mg·L−1 Cs时,抗氧化酶活性显著降低。Cs积累具有剂量和组织依赖性,叶片Cs积累量(536.10 μg·g−1)高于茎(413.74 μg·1)和根(284.69 μg·1)。结果表明,马齿苋是一种超积累Cs的植物,可用于修复Cs污染土壤。
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引用次数: 3
Effect of Presprouting Plant Growth Regulators and Natural Materials on Dormancy, Growth, and Yield of Potatoes (Solanum tuberosum L.) 发芽前植物生长调节剂和天然物质对马铃薯休眠、生长和产量的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-18 DOI: 10.1155/2022/9932606
M. Moletsane, P. Kimurto, M. Oyoo
Irish potatoes are amongst the most highly grown and demanded crops in Kenya for food, industrial starch, and animal feed. Farmers, however, face a serious challenge regarding the timely availability of well-sprouted seed potato tubers due to the physiological seed dormancy period of 2–3 months, thereby reducing production cycles. This study determined the effects of different chitting methods on enhancing the presprouting of different potato varieties in Kenya. Plant growth regulators (PGR) (Gibberellins (GA3), 6-Benzylaminopurine, and Zeatin) and natural materials (grass, banana leaves, and soil) were evaluated for their effects in breaking dormancy and stimulating the growth of sprouts under greenhouse conditions in a complete randomized design (CRD) with three replicates. The evaluation of the presprouted seed in the field was conducted at Egerton University and Kenya Agricultural and Livestock Research Organization (KALRO), Molo, in a split-plot design for two seasons. Data was taken on crop emergence, length, thickness, and colour of sprouts, plant height, tubers per plant, tuber thickness, and tuber yield. Data were subjected to a general linear model to partition the variance component using SAS software version 9.13, and means were separated using the least significant difference ( p ≤ 0.05 ). There were significant ( p ≤ 0.05 ) main effects on the prespouting time, growth, and yield of tubers. The interaction effects due to variety and treatment were also significant ( p ≤ 0.05 ) for sprout thickness. Natural materials produced the most vigorous sprouts, increased crop emergence, plant height, and superior tuber yield. Natural materials and PGRs increased tuber size for chitted potato seed by 261% and 103%, respectively. Control treatments had a significantly higher frequency of small-sized tubers than natural materials and PGRs, proving the importance of chitting in increasing tuber size and yields. Natural materials increased sprout quality (thickness and length) better than PRGs and control treatments. This study showed that small-holder farmers could adopt the use of readily available soil, grass, and banana leaves while large-scale growers, with access to better facilities, could use PGRs to break tuber dormancy for increased potato tuber yield.
爱尔兰马铃薯是肯尼亚种植最多、需求量最大的作物之一,可作为食品、工业淀粉和动物饲料。然而,由于马铃薯种子的生理休眠期为2-3个月,从而缩短了生产周期,农民在及时获得发芽良好的马铃薯块茎方面面临着严峻的挑战。本研究确定了在肯尼亚不同的马铃薯品种中,不同的催芽方法对提高其发芽的效果。采用3个完全随机设计(CRD),研究了温室条件下植物生长调节剂(赤霉素(giberellins, GA3)、6-苄基氨基嘌呤(6-Benzylaminopurine)和玉米素(Zeatin)和天然材料(草、香蕉叶和土壤)对破休眠和促进芽苗生长的影响。Egerton大学和位于Molo的肯尼亚农畜研究组织(KALRO)采用两个季节的分块设计,在田间对预发芽种子进行了评价。数据包括作物出苗、芽的长度、厚度和颜色、株高、每株块茎、块茎厚度和块茎产量。采用SAS 9.13版软件对数据进行一般线性模型划分方差成分,采用最小显著差异(p≤0.05)进行均值分离。对块茎发芽前时间、生长和产量有显著(p≤0.05)的主要影响。品种和处理对芽粗的互作效应也显著(p≤0.05)。天然材料产生最有力的芽,增加作物出苗率,株高,和优越的块茎产量。天然材料和pgr分别使马铃薯马铃薯种子块茎大小增加261%和103%。对照处理产生小块茎的频率显著高于天然材料和pgr处理,证明了劈裂在提高块茎大小和产量方面的重要性。天然材料比PRGs和对照处理更能提高芽质量(厚度和长度)。这项研究表明,小农可以利用现成的土壤、草和香蕉叶,而拥有更好设施的大规模种植者可以利用pgr打破块茎休眠,以提高马铃薯块茎产量。
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引用次数: 1
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Advances in Agriculture
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