Pub Date : 2023-04-03DOI: 10.24843/metamorfosa.2023.v10.i01.p09
Ichsan Luqmana Indra Putra, Winda Aulia Putri
Capung berperan sebagai predator hama serta sebagai bioindikator perairan sehingga perlu dijaga kelestariannya. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis, karakteristik spesies, serta spesies capung yang melimpah dan tidak melimpah di sekitar kampus 4 UAD. Lokasi sampling dibagi menjadi empat, yaitu persawahan, perumahan, lahan kosong dan area kampus. Setiap area terdapat 2 plot berukuran 35 x 20 m. Setiap plot memiliki 5 subplot berukuran 150 m2 yang tersebar dalam plot. Pengambilan sampel dilakukan pada pukul 08.00 - 11.00 WIB dan pukul 15.00 – 17.00 WIB. Pengambilan sampel dilakukan dengan menggunakan jaring. Capung yang tertangkap dimasukkan kertas papilot dan ditekan toraksnya hingga mati. Capung kemudian diidentifikasi sampai tingkat spesies dengan membandingkan morfologinya dengan buku acuan identifikasi. Faktor abiotik yang diukur berupa suhu dan kelembapan udara, kecepatan angin, CO, DO serta pH air. Data yang didapatkan dianalisis menggunakan analisis deskriptif inferensial. Indeks keanekaragaman dihitung dengan rumus Shannon - Wiener dan indeks dominansi dengan rumus Simpson. Hasil penelitian ini didapatkan 4 jenis capung yaitu Pantala flavescens, Orthretum sabina, Sympetrum fonscolombii dan Diplacodes trivialis dengan memiliki tingkat keanekaragaman rendah yaitu 0,36. Spesies capung yang paling melimpah yaitu Sympetrum fonscolombii sebanyak 249 ekor dengan nilai indeks dominansi sebesar 0,74 dan yang tidak melimpah yaitu Diplacodes trivialis sebanyak 10 ekor dengar nilai indeks dominansi sebesar 0,03. Kesimpulan dari penelitian ini pada area sekitar kampus 4 UAD terdapat adanya gangguan berupa alih fungsi lahan yang menyebabkan kecilnya indeks keanekaragaman jenis capung pada lokasi tersebut.
{"title":"Keanekaragaman Jenis Capung (Hexapoda: Odonata) Di Sekitar Kampus 4 Universitas Ahmad Dahlan, Yogyakarta","authors":"Ichsan Luqmana Indra Putra, Winda Aulia Putri","doi":"10.24843/metamorfosa.2023.v10.i01.p09","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p09","url":null,"abstract":"Capung berperan sebagai predator hama serta sebagai bioindikator perairan sehingga perlu dijaga kelestariannya. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis, karakteristik spesies, serta spesies capung yang melimpah dan tidak melimpah di sekitar kampus 4 UAD. Lokasi sampling dibagi menjadi empat, yaitu persawahan, perumahan, lahan kosong dan area kampus. Setiap area terdapat 2 plot berukuran 35 x 20 m. Setiap plot memiliki 5 subplot berukuran 150 m2 yang tersebar dalam plot. Pengambilan sampel dilakukan pada pukul 08.00 - 11.00 WIB dan pukul 15.00 – 17.00 WIB. Pengambilan sampel dilakukan dengan menggunakan jaring. Capung yang tertangkap dimasukkan kertas papilot dan ditekan toraksnya hingga mati. Capung kemudian diidentifikasi sampai tingkat spesies dengan membandingkan morfologinya dengan buku acuan identifikasi. Faktor abiotik yang diukur berupa suhu dan kelembapan udara, kecepatan angin, CO, DO serta pH air. Data yang didapatkan dianalisis menggunakan analisis deskriptif inferensial. Indeks keanekaragaman dihitung dengan rumus Shannon - Wiener dan indeks dominansi dengan rumus Simpson. Hasil penelitian ini didapatkan 4 jenis capung yaitu Pantala flavescens, Orthretum sabina, Sympetrum fonscolombii dan Diplacodes trivialis dengan memiliki tingkat keanekaragaman rendah yaitu 0,36. Spesies capung yang paling melimpah yaitu Sympetrum fonscolombii sebanyak 249 ekor dengan nilai indeks dominansi sebesar 0,74 dan yang tidak melimpah yaitu Diplacodes trivialis sebanyak 10 ekor dengar nilai indeks dominansi sebesar 0,03. Kesimpulan dari penelitian ini pada area sekitar kampus 4 UAD terdapat adanya gangguan berupa alih fungsi lahan yang menyebabkan kecilnya indeks keanekaragaman jenis capung pada lokasi tersebut.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42769038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-03DOI: 10.24843/metamorfosa.2023.v10.i01.p10
Y. R. Irawan, N. N. Wirasiti, N. Suarni, A. Sukmaningsih
Juwet fruit is high antioxidants that can increase reproductive ability. This study aims to see the effect of juwet fruit extract on the morphology and ratio of X and Y spermatozoa after exposure to cigarette smoke. This study used a completely randomized design (CRD) with 5 treatments namely K-, K+ (given 1 commercial cigarette/day), P1 (given juwet fruit extract 1.8 mg/g BW and one commercial cigarette/day), P2 ( given juwet fruit extract 1.8 mg/g BW) and P3 (given one cigarette with juwet fruit extract filter/day) and each treatment had 4 replications. The parameters observed in this study were spermatozoa with abnormal morphology and the ratio of X : Y spermatozoa. The results showed that there was a significant difference in the percentage of abnormal spermatozoa between the control and treatment groups. In this study, juwet fruit extract did not significantly affect the ratio of X and Y spermatozoa. It can be concluded that juwet fruit extract is able to reduce the risk of morphological abnormalities in spermatozoa after exposure to cigarette smoke. However, these three methods have not been able to effectively affect the ratio of X and Y spermatozoa after exposure to cigarette smoke. Keyword: cigarettes, free radicals, juwet extract, mice, spermatozoa ratio.
{"title":"Morfologi Dan Rasio Spermatozoa X : Y Mencit (Mus Musculus) Yang Diberi Ekstrak Buah Juwet (Syzygium Cumini) Setelah Terpapar Asap Rokok","authors":"Y. R. Irawan, N. N. Wirasiti, N. Suarni, A. Sukmaningsih","doi":"10.24843/metamorfosa.2023.v10.i01.p10","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p10","url":null,"abstract":"Juwet fruit is high antioxidants that can increase reproductive ability. This study aims to see the effect of juwet fruit extract on the morphology and ratio of X and Y spermatozoa after exposure to cigarette smoke. This study used a completely randomized design (CRD) with 5 treatments namely K-, K+ (given 1 commercial cigarette/day), P1 (given juwet fruit extract 1.8 mg/g BW and one commercial cigarette/day), P2 ( given juwet fruit extract 1.8 mg/g BW) and P3 (given one cigarette with juwet fruit extract filter/day) and each treatment had 4 replications. The parameters observed in this study were spermatozoa with abnormal morphology and the ratio of X : Y spermatozoa. The results showed that there was a significant difference in the percentage of abnormal spermatozoa between the control and treatment groups. In this study, juwet fruit extract did not significantly affect the ratio of X and Y spermatozoa. It can be concluded that juwet fruit extract is able to reduce the risk of morphological abnormalities in spermatozoa after exposure to cigarette smoke. However, these three methods have not been able to effectively affect the ratio of X and Y spermatozoa after exposure to cigarette smoke. \u0000Keyword: cigarettes, free radicals, juwet extract, mice, spermatozoa ratio.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46330659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-03DOI: 10.24843/metamorfosa.2023.v10.i01.p08
Maria Matoetina Suprijon, T. E. W. Widyastuti, Anna Ingani Widjajaseputra
Jawa timur adalah salah satu sentra produksi ubi jalar oranye Cilembu yang memiliki kadar beta-karoten paling tinggi daripada ubi jalar lainnya. Ubi jalar segar memiliki masa simpan terbatas sehingga diolah menjadi tepung tetapi di sisi lain dapat menyebabkan degradasi warna tepung ubi karena cahaya, oksigen, sinar UV dan pemanasan. Oleh karena itu perlu diteliti penggunaan suhu dan penyiapan ubi jalar oranye yang akan dikeringkan yang masih dapat mempertahankan sifat fisikokimia, sekaligus memiliki sifat fungsional yang dibutuhkan dalam pengolahan tepung menjadi produk selanjutnya. Sampel ubi jalar oranye Cilembu yang digunakan dalam penelitian ini umumnya berbentuk seperti gelondong dengan rata-rata panjang ubi 17.27+2.64 cm, diameter bagian atas 4.28+0.72 cm, tengah 7.04+0.69 cm dan bawah 5.08+1.12 cm. Pengeringan sampel ubi dengan cabinet drier pada suhu 50?C menghasilkan rendemen tepung hasil perlakuan irisan adalah 16.78% dan dari perlakuan pemarutan 6.34%. Perbedaan pelakuan irisan dan parutan sebelum pengeringan mempengaruhi warna, profil gelatinisasi, dan viskositas sampel tepung ubi, tetapi tidak mempengaruhi daya serap air-nya. Daya serap air kedua tepung adalah 1.90+0.05 ml/g. Warna kedua jenis tepung tampak lebih pucat dari warna ubi jalar segar, tetapi nilai L* tepung perlakuan parutan lebih tinggi daripada tepung perlakuan irisan. Nilai a* dan b* menurun nyata walau masih bernilai positif yang berarti masih berwarna oranye. Berdasarkan nilai L*, a*, b* perlakuan irisan lebih dapat mempertahankan warna sampel tepung ubi hasil irisan lebih baik (lebih oranye) daripada perlakuan parutan. Pemarutan menyebabkan tepung sampel ubi memiliki ukuran granula pati tepung sangat bervariasi. Hal ini mempengaruhi profil gelatinisasi dan viskositas yang tidak stabil.
{"title":"KARAKTERISTIK Karakteristik Fisikokimia Tepung Ubi Jalar Varietas Cilembu dari Proses Penepungan yang Berbeda","authors":"Maria Matoetina Suprijon, T. E. W. Widyastuti, Anna Ingani Widjajaseputra","doi":"10.24843/metamorfosa.2023.v10.i01.p08","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p08","url":null,"abstract":"Jawa timur adalah salah satu sentra produksi ubi jalar oranye Cilembu yang memiliki kadar beta-karoten paling tinggi daripada ubi jalar lainnya. Ubi jalar segar memiliki masa simpan terbatas sehingga diolah menjadi tepung tetapi di sisi lain dapat menyebabkan degradasi warna tepung ubi karena cahaya, oksigen, sinar UV dan pemanasan. Oleh karena itu perlu diteliti penggunaan suhu dan penyiapan ubi jalar oranye yang akan dikeringkan yang masih dapat mempertahankan sifat fisikokimia, sekaligus memiliki sifat fungsional yang dibutuhkan dalam pengolahan tepung menjadi produk selanjutnya. Sampel ubi jalar oranye Cilembu yang digunakan dalam penelitian ini umumnya berbentuk seperti gelondong dengan rata-rata panjang ubi 17.27+2.64 cm, diameter bagian atas 4.28+0.72 cm, tengah 7.04+0.69 cm dan bawah 5.08+1.12 cm. Pengeringan sampel ubi dengan cabinet drier pada suhu 50?C menghasilkan rendemen tepung hasil perlakuan irisan adalah 16.78% dan dari perlakuan pemarutan 6.34%. Perbedaan pelakuan irisan dan parutan sebelum pengeringan mempengaruhi warna, profil gelatinisasi, dan viskositas sampel tepung ubi, tetapi tidak mempengaruhi daya serap air-nya. Daya serap air kedua tepung adalah 1.90+0.05 ml/g. Warna kedua jenis tepung tampak lebih pucat dari warna ubi jalar segar, tetapi nilai L* tepung perlakuan parutan lebih tinggi daripada tepung perlakuan irisan. Nilai a* dan b* menurun nyata walau masih bernilai positif yang berarti masih berwarna oranye. Berdasarkan nilai L*, a*, b* perlakuan irisan lebih dapat mempertahankan warna sampel tepung ubi hasil irisan lebih baik (lebih oranye) daripada perlakuan parutan. Pemarutan menyebabkan tepung sampel ubi memiliki ukuran granula pati tepung sangat bervariasi. Hal ini mempengaruhi profil gelatinisasi dan viskositas yang tidak stabil.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68901500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.24843/metamorfosa.2023.v10.i01.p07
R. Oktarianti, Siti Agustina Wulandari, Hidayat teguh Wiyono, Mahriani Mahriani
The taste of sensitivity of phenylthiocarbamide (PTC), is autosomal dominant trait inherited. The taster controled by dominant allele while the non taster determinated by recessive allele. The purpose of the study is to investigate the prevalence of taster and non taster, gene frequencyof taster and non taster and pedegree analysis of non taster samples of Gili Ketapang island population in Probolinggo regency.. Determination of the sample was carry out randomly. Detection of the ability to taste PTC is done by asking respondents to feel the PTC solution. The PTC solution used was from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). The results showed that the prevalence of tasters and non-tasters were 96.88% and 3.12%, respectively. The non-taster (t) and taster (T) alleles frequencies were 0.17 and 0.83 respectively. The results of pedegree analysis show that non-taster are born from heterozygous taster pairs (husband and wife are Tt) or from heterozygous taster pairs (Tt) with non-taster (tt). Keyword: Phenylthiocarbamide (PTC), taster, non taster. Metamorfosa:Journal of Biological Sciences 10(1): 67-74 (Maret 2023) eISSN: 2655-8122
{"title":"Prevalensi Kemampuan Mengecap Phenylthiocarbamide (PTC) Pada Penduduk Pulau Gili Ketapang Kabupaten Probolinggo","authors":"R. Oktarianti, Siti Agustina Wulandari, Hidayat teguh Wiyono, Mahriani Mahriani","doi":"10.24843/metamorfosa.2023.v10.i01.p07","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p07","url":null,"abstract":"The taste of sensitivity of phenylthiocarbamide (PTC), is autosomal dominant trait inherited. The taster controled by dominant allele while the non taster determinated by recessive allele. The purpose of the study is to investigate the prevalence of taster and non taster, gene frequencyof taster and non taster and pedegree analysis of non taster samples of Gili Ketapang island population in Probolinggo regency.. Determination of the sample was carry out randomly. Detection of the ability to taste PTC is done by asking respondents to feel the PTC solution. The PTC solution used was from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). The results showed that the prevalence of tasters and non-tasters were 96.88% and 3.12%, respectively. The non-taster (t) and taster (T) alleles frequencies were 0.17 and 0.83 respectively. The results of pedegree analysis show that non-taster are born from heterozygous taster pairs (husband and wife are Tt) or from heterozygous taster pairs (Tt) with non-taster (tt). Keyword: Phenylthiocarbamide (PTC), taster, non taster. Metamorfosa:Journal of Biological Sciences 10(1): 67-74 (Maret 2023) eISSN: 2655-8122","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46050703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.24843/metamorfosa.2023.v10.i01.p06
Kadek Andina Widiastuti, L. P. E. K. Yuni, Ida Ayu Astarini
ABSTRACT Coral transplantation is the activity of cultivating/breeding coral colonies using the fragmentation method. This activity is carried out as an effort to prevent damage to the coral reef ecosystem so the existence of coral reefs and the species that live around them can be maintained. Several techniques have been used in transplant activities, but knowledge of the right transplantation technique is needed so the activities can run well. This research aims to determine the effect of different types of substrates used on the growth and growth rate of coral Stylophora pistillata transplanted at three depths at Serangan Beach, so the transplantation activities run effectively and efficiently. The research was carried out for four months from November 2021 to March 2022 through field research using the Randomized Block Design method with two factors, namely the type of substrate used (cement and sand; bricks) and different depths (1 m, 3 m and 5 m). This study used 90 fragments of Stylophora pistillata. The results of the analysis showed that the different types of substrate gave significantly different effects on growth and growth rate at a depth of 1 m. However, at the other two depths, the effect of different substrate types were not differed significantly. The best coral growth and growth rate was seen in corals using cement and sand substrates transplanted at a depth of 1 m with growth of 3,29 cm (height) and 3,93 cm (width), while the highest growth rate was 0,82 cm/ month. (height) and 0,98 cm/month (width).
{"title":"Pertumbuhan Dan Laju Pertumbuhan Karang Stylophora pistillata Dengan Jenis Substrat Berbeda Yang Ditanam Pada Tiga Kedalaman Di Pantai Serangan","authors":"Kadek Andina Widiastuti, L. P. E. K. Yuni, Ida Ayu Astarini","doi":"10.24843/metamorfosa.2023.v10.i01.p06","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p06","url":null,"abstract":"ABSTRACT \u0000Coral transplantation is the activity of cultivating/breeding coral colonies using the fragmentation method. This activity is carried out as an effort to prevent damage to the coral reef ecosystem so the existence of coral reefs and the species that live around them can be maintained. Several techniques have been used in transplant activities, but knowledge of the right transplantation technique is needed so the activities can run well. This research aims to determine the effect of different types of substrates used on the growth and growth rate of coral Stylophora pistillata transplanted at three depths at Serangan Beach, so the transplantation activities run effectively and efficiently. The research was carried out for four months from November 2021 to March 2022 through field research using the Randomized Block Design method with two factors, namely the type of substrate used (cement and sand; bricks) and different depths (1 m, 3 m and 5 m). This study used 90 fragments of Stylophora pistillata. The results of the analysis showed that the different types of substrate gave significantly different effects on growth and growth rate at a depth of 1 m. However, at the other two depths, the effect of different substrate types were not differed significantly. The best coral growth and growth rate was seen in corals using cement and sand substrates transplanted at a depth of 1 m with growth of 3,29 cm (height) and 3,93 cm (width), while the highest growth rate was 0,82 cm/ month. (height) and 0,98 cm/month (width).","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42851670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.24843/metamorfosa.2023.v10.i01.p05
Risqa Izzatul Zulfa, M. Joni, I. Wijaya
Corn (Zea mays L.) is one of the most important carbohydrate-producing food crops in the world. One of the problems in corn production is a decrease in the quantity and quality of corn due to the presence of weeds that grow around corn fields. This study aims to determine the structure and composition of weeds in the corn fields of Belayu Village, Marga District, Tabanan Regency, Bali Province. The method used in this study is the permanent quadrat method with plot size 1x1 m. Data were analyzed using vegetation parameters consisting of Important Value Index (IVI), diversity index (H'), dominance index (C), evenness index (E), and similarity index (IS). The results obtained were the composition of weeds found in the 3rd week, namely 18 species from 9 families, at the 6th week, 25 species from 16 families, and at the 9th week, 31 species from 19 families. The highest IVI values ??were found in Cyperus rotundus L of the Cyperaceae families at the 3rd, 6th, and 9th weeks. Biodiversity (H') index is moderate ranges from 2.29 to 2.77, the dominance index (C) indicates that no weeds dominate with values ??ranging from 0.09-0.13 The evenness index (E) indicates that the species Weed types were classified as evenly distributed with values ??ranging from 0.78-0.80, and a similarity index (IS) of 72.9%, indicating that the similarity of weed species in each category was classified as high.
{"title":"Structure and Composition of Weed In Corn Fields (Zea mays L.) Village Belayu Marga District Tabanan Regency Bali Province","authors":"Risqa Izzatul Zulfa, M. Joni, I. Wijaya","doi":"10.24843/metamorfosa.2023.v10.i01.p05","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p05","url":null,"abstract":"Corn (Zea mays L.) is one of the most important carbohydrate-producing food crops in the world. One of the problems in corn production is a decrease in the quantity and quality of corn due to the presence of weeds that grow around corn fields. This study aims to determine the structure and composition of weeds in the corn fields of Belayu Village, Marga District, Tabanan Regency, Bali Province. The method used in this study is the permanent quadrat method with plot size 1x1 m. Data were analyzed using vegetation parameters consisting of Important Value Index (IVI), diversity index (H'), dominance index (C), evenness index (E), and similarity index (IS). The results obtained were the composition of weeds found in the 3rd week, namely 18 species from 9 families, at the 6th week, 25 species from 16 families, and at the 9th week, 31 species from 19 families. The highest IVI values ??were found in Cyperus rotundus L of the Cyperaceae families at the 3rd, 6th, and 9th weeks. Biodiversity (H') index is moderate ranges from 2.29 to 2.77, the dominance index (C) indicates that no weeds dominate with values ??ranging from 0.09-0.13 The evenness index (E) indicates that the species Weed types were classified as evenly distributed with values ??ranging from 0.78-0.80, and a similarity index (IS) of 72.9%, indicating that the similarity of weed species in each category was classified as high.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47675750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Makanan dan minuman fungsional dengan banyak manfaat kesehatan sangatlah penting untuk meningkatkan imun tubuh paska pandemi Covid-19. Salah satu tanaman kesehatan yang sudah terkenal sejak lama adalah teh hijau (Camellia sinensis L.). Sitotoksisitas teh hijau baik pada sel normal maupun sel penyakit, masih terus dipelajari sampai dengan saat ini. Penelitian ini bertujuan untuk melihat viabilitas sel makrofag RAW 264.7 yang diberi perlakuan ekstrak teh hijau yang diperoleh dari Pusat Penelitian Teh dan Kina (PPTK) Gambung, Jawa Barat. Penelitian ini merupakan penelitian pendahuluan untuk mencari konsentrasi yang aman digunakan pada sel RAW 264.7 secara in vitro. Sel makrofag RAW 264.7 diberi perlakuan ekstrak teh hijau dengan konsentrasi 12,5, 25, 50, dan 75 µg/ml, dan juga kuersetin 12,5, 25, 50, dan 75 µM sebagai senyawa pembanding. Viabilitas diukur dengan menggunakan analisis 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Perlakukan ekstrak teh hijau pada konsentrasi 12,5, 25, and 50 µg/ml menujukkan viabilitas sel makrofag RAW 264.7 di atas 90% yang berarti konsentrasi tersebut tidak bersifat toksik terhadap sel RAW 264.7 sehingga dapat digunakan untuk uji efikasi ekstrak teh hijau.
{"title":"Sitotoksisitas Ekstrak Teh Hijau (Camellia sinensis L.) Dari Gambung, Jawa Barat Terhadap Sel Makrofag Raw 264.7","authors":"Arina Novilla, Gina Khairinisa, Dwi Davidson Rihibiha, Heri Syahrian, S. Shabri","doi":"10.24843/metamorfosa.2023.v10.i01.p03","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p03","url":null,"abstract":"Makanan dan minuman fungsional dengan banyak manfaat kesehatan sangatlah penting untuk meningkatkan imun tubuh paska pandemi Covid-19. Salah satu tanaman kesehatan yang sudah terkenal sejak lama adalah teh hijau (Camellia sinensis L.). Sitotoksisitas teh hijau baik pada sel normal maupun sel penyakit, masih terus dipelajari sampai dengan saat ini. Penelitian ini bertujuan untuk melihat viabilitas sel makrofag RAW 264.7 yang diberi perlakuan ekstrak teh hijau yang diperoleh dari Pusat Penelitian Teh dan Kina (PPTK) Gambung, Jawa Barat. Penelitian ini merupakan penelitian pendahuluan untuk mencari konsentrasi yang aman digunakan pada sel RAW 264.7 secara in vitro. Sel makrofag RAW 264.7 diberi perlakuan ekstrak teh hijau dengan konsentrasi 12,5, 25, 50, dan 75 µg/ml, dan juga kuersetin 12,5, 25, 50, dan 75 µM sebagai senyawa pembanding. Viabilitas diukur dengan menggunakan analisis 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). Perlakukan ekstrak teh hijau pada konsentrasi 12,5, 25, and 50 µg/ml menujukkan viabilitas sel makrofag RAW 264.7 di atas 90% yang berarti konsentrasi tersebut tidak bersifat toksik terhadap sel RAW 264.7 sehingga dapat digunakan untuk uji efikasi ekstrak teh hijau.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43132308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-28DOI: 10.24843/metamorfosa.2023.v10.i01.p04
Yohana Deni Suryati, I. Setyawati, D. S. Yusup
Penelitian bertujuan untuk mengkaji aspek morfometri dan biologi reproduksi ikan tongkol lisong (Auxis rochei, Risso 1810) yang didaratkan di Pangkalan Pendaratan Ikan (PPI) Kedonganan. Aspek morfometri meliputi sebaran panjang dan hubungan panjang berat ikan, sedangkan kematangan gonad diamati secara histologis berdasarkan perkembangan sel gonad. Hasil penelitian menunjukkan ikan tongkol lisong memiliki sebaran panjang antara 16,5 - 35,3 cmFL dan berat tubuh antara 64,8 - 539,4 gram dengan pola pertumbuhan ikan tongkol lisong bersifat allometrik positif (b>3). Secara umum ikan tongkol lisong yang didaratkan di PPI Kedonganan didominasi ikan yang memiliki TKG sudah matang gonad (TKG III, IV dan V) sebanyak 123 ekor (58,85 %) pada kisaran panjang antara 22 - 23 cmFL. Perkembangan gonad yang tidak bersamaan mengindikasikan bahwa reproduksi ikan tongkol lisong bersifat asynchrounous (pemijahan berganda / multiple spawning) dengan periode mijah tidak sekaligus (partial spawning). Kata kunci : tongkol lisong, hubungan panjang - berat, aspek reproduksi, gonad
{"title":"Analisis Aspek Morfometri Dan Biologi Reproduksi Tongkol Lisong (Auxis rochei) yang Didaratkan Di Ppi Kedonganan, Bali","authors":"Yohana Deni Suryati, I. Setyawati, D. S. Yusup","doi":"10.24843/metamorfosa.2023.v10.i01.p04","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p04","url":null,"abstract":"Penelitian bertujuan untuk mengkaji aspek morfometri dan biologi reproduksi ikan tongkol lisong (Auxis rochei, Risso 1810) yang didaratkan di Pangkalan Pendaratan Ikan (PPI) Kedonganan. Aspek morfometri meliputi sebaran panjang dan hubungan panjang berat ikan, sedangkan kematangan gonad diamati secara histologis berdasarkan perkembangan sel gonad. Hasil penelitian menunjukkan ikan tongkol lisong memiliki sebaran panjang antara 16,5 - 35,3 cmFL dan berat tubuh antara 64,8 - 539,4 gram dengan pola pertumbuhan ikan tongkol lisong bersifat allometrik positif (b>3). Secara umum ikan tongkol lisong yang didaratkan di PPI Kedonganan didominasi ikan yang memiliki TKG sudah matang gonad (TKG III, IV dan V) sebanyak 123 ekor (58,85 %) pada kisaran panjang antara 22 - 23 cmFL. Perkembangan gonad yang tidak bersamaan mengindikasikan bahwa reproduksi ikan tongkol lisong bersifat asynchrounous (pemijahan berganda / multiple spawning) dengan periode mijah tidak sekaligus (partial spawning). \u0000 \u0000 Kata kunci : tongkol lisong, hubungan panjang - berat, aspek reproduksi, gonad","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44667768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.24843/metamorfosa.2023.v10.i01.p02
Vanisa Sri Elvani, Syauqi Susana Rahmani, Safrida Dwiningsih, Tetty Marta Linda, Windi Dona Fitri Fitri
{"title":"Identifikasi Molekuler Bakteri Endofit Tanaman Karet (Hevea Brasiliensis) dan Aktivitasnya dalam Memproduksi Inhibitor Alfa Amilase","authors":"Vanisa Sri Elvani, Syauqi Susana Rahmani, Safrida Dwiningsih, Tetty Marta Linda, Windi Dona Fitri Fitri","doi":"10.24843/metamorfosa.2023.v10.i01.p02","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p02","url":null,"abstract":"","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43141220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-27DOI: 10.24843/metamorfosa.2023.v10.i01.p01
I. G. A. A. S. Ekaryani, A. Indraningrat, Ni Made Ayu Suardani Singapurwa, I. Sudiarta, A. A. M. Semariyani, I. Candra
{"title":"Isolasi Bakteri Dari Rumput Laut Eucheuma spinosum dan Skrining Aktivitas Antibakteri Terhadap Beberapa Bakteri Gram Positif dan Negatif","authors":"I. G. A. A. S. Ekaryani, A. Indraningrat, Ni Made Ayu Suardani Singapurwa, I. Sudiarta, A. A. M. Semariyani, I. Candra","doi":"10.24843/metamorfosa.2023.v10.i01.p01","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p01","url":null,"abstract":"","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47091676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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