Pub Date : 2022-10-14DOI: 10.24843/metamorfosa.2022.v09.i02.p04
Maghfirotun Nisa, M. Proborini, I. Astarini
Tujuan penelitian adalah untuk mengetahui pengaruh pemberian kombinasi endomikoriza Glomus dan jamur Trichoderma serta dosis yang sesuai untuk meningkatkan produktivitas tanaman jagung (Zea mays L.). Penelitian diawali dengan perbanyakan spora endomikoriza Glomus di green house Program Studi Biologi Fakultas MIPA Universitas Udayana dan dilanjutkan dengan penyaringan spora endomikoriza, reisolasi jamur Trichoderma serta uji endomikoriza Glomus dan Trichoderma pada tanaman jagung. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan secara signifikan antara kontrol positif (KNO3) dan perlakuan endomikoriza dan Trichoderma pada tinggi tanaman, jumlah daun, panjang akar, panjang tongkol, diameter tongkol, berat basah dan kering tongkol dan berat pipilan serta berbeda nyata pada besar kecil pipilan. Dosis 125 spora Glomus dan 5 mL Trichoderma memberikan hasil terbaik pada besar kecil pipilan jagung dibandingkan dosis lainnya, sehingga kombinasi endomikoriza dan Trichoderma dapat diaplikasikan untuk mengurangi pupuk kimia yang berlebihan. Kata kunci: pertumbuhan, produktivitas, pupuk hayati, unsur hara
{"title":"The Effect Combination of Glomus Endomycorrhizal and Trichoderma on The Productivity of Corn (Zea Mays L.)","authors":"Maghfirotun Nisa, M. Proborini, I. Astarini","doi":"10.24843/metamorfosa.2022.v09.i02.p04","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p04","url":null,"abstract":"Tujuan penelitian adalah untuk mengetahui pengaruh pemberian kombinasi endomikoriza Glomus dan jamur Trichoderma serta dosis yang sesuai untuk meningkatkan produktivitas tanaman jagung (Zea mays L.). Penelitian diawali dengan perbanyakan spora endomikoriza Glomus di green house Program Studi Biologi Fakultas MIPA Universitas Udayana dan dilanjutkan dengan penyaringan spora endomikoriza, reisolasi jamur Trichoderma serta uji endomikoriza Glomus dan Trichoderma pada tanaman jagung. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan secara signifikan antara kontrol positif (KNO3) dan perlakuan endomikoriza dan Trichoderma pada tinggi tanaman, jumlah daun, panjang akar, panjang tongkol, diameter tongkol, berat basah dan kering tongkol dan berat pipilan serta berbeda nyata pada besar kecil pipilan. Dosis 125 spora Glomus dan 5 mL Trichoderma memberikan hasil terbaik pada besar kecil pipilan jagung dibandingkan dosis lainnya, sehingga kombinasi endomikoriza dan Trichoderma dapat diaplikasikan untuk mengurangi pupuk kimia yang berlebihan. \u0000Kata kunci: pertumbuhan, produktivitas, pupuk hayati, unsur hara","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41643378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-13DOI: 10.24843/metamorfosa.2022.v09.i02.p03
Putu Mila Ayustina, Ni Made Rai Suarni, Ni Gusti Ayu Manik Ermayanti
Meloxicam is a Non-Steroidal Anti-Inflammatory Drug (NSAID) given to humans and animals to treat inflammation that is most widely prescribed. The use of meloxicam that is not in accordance with the dose and in the long term will cause a toxic effect of meloxicam. Meloxicam can trigger oxidative stress and has a mechanism of inhibition of prostaglandin synthesis that can affect hormonal regulation and male reproduction. The purpose of this study was to analyze potential of (Moringa oleifera) leaf ethanol extract on sperm quality and testosterone hormone of male white rats (Rattus norvegicus) induced meloxicam. The design used was a (CRD) Completely Randomized Design consisting of three treatments and two controls with six replications. The treatment was given for 35 days. Meloxicam was given at a dose of 8,4 mg/kg body weight and moringa extract at a dose of 200 mg/kg body weight/day (P1), 400 mg/kg body weight/day (P2), and 600 mg/kg body weight/day (P3). The results showed that moringa extract dose of 400 mg/kgBW and 600 mg/kgBW has the potential to increase reproductive organ weight, motility, spermatozoa viability, spermatozoa count, and increase testosterone levels significantly (P<0,05).
{"title":"Potensi Ekstrak Etanol Daun Kelor (Moringa Oleifera) Terhadap Kualitas Spermatozoa dan Hormon Testosteron Tikus Putih Jantan yang Diinduksi Meloxicam","authors":"Putu Mila Ayustina, Ni Made Rai Suarni, Ni Gusti Ayu Manik Ermayanti","doi":"10.24843/metamorfosa.2022.v09.i02.p03","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p03","url":null,"abstract":"Meloxicam is a Non-Steroidal Anti-Inflammatory Drug (NSAID) given to humans and animals to treat inflammation that is most widely prescribed. The use of meloxicam that is not in accordance with the dose and in the long term will cause a toxic effect of meloxicam. Meloxicam can trigger oxidative stress and has a mechanism of inhibition of prostaglandin synthesis that can affect hormonal regulation and male reproduction. The purpose of this study was to analyze potential of (Moringa oleifera) leaf ethanol extract on sperm quality and testosterone hormone of male white rats (Rattus norvegicus) induced meloxicam. The design used was a (CRD) Completely Randomized Design consisting of three treatments and two controls with six replications. The treatment was given for 35 days. Meloxicam was given at a dose of 8,4 mg/kg body weight and moringa extract at a dose of 200 mg/kg body weight/day (P1), 400 mg/kg body weight/day (P2), and 600 mg/kg body weight/day (P3). The results showed that moringa extract dose of 400 mg/kgBW and 600 mg/kgBW has the potential to increase reproductive organ weight, motility, spermatozoa viability, spermatozoa count, and increase testosterone levels significantly (P<0,05).","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43175338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.24843/metamorfosa.2022.v09.i02.p01
Ni Putu Eny Sulistya Dewi, N. I. Wiratmini, J. Sudiman
Oocyte developmental competence is one of the determining factors that in?uence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis of oocyte cumulus cells (CCs) can be used as a method to predict oocyte competence and viability that could potentially function as molecular predictors for IVF program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 and BAX) that are expressed in human CCs between mature (MII) and immature (MI) oocytes. This research is an analytic observational study. Twelve samples of CCs from mature (MII) and immature (MI) oocytes were collected after ovum pick up from patients undergoing ICSI. CCs samples were isolated using an ultrasonicator and protein expressions were quantified using ELISA method. The Mann-Whitney test was used to compare the protein expressions from CCs between mature and immature oocytes. The results showed HAS2, PTX3 and BCL2 proteins expressions was increase in CCs from mature oocytes but had no significant effect compared to CCs from immature oocytes, however, BAX protein expression was significantly higher (P<0.05) in CCs from immature oocytes which CCs from mature oocytes. This present study shows that BAX proteins could be used as markers for oocyte quality and maturation. Key words: ELISA, maturation, proteins marker, cumulus cells (CCs)
{"title":"Kuantifikasi Protein HAS2, PTX3, BCL2 dan BAX dalam Sel Kumulus Oosit Matur (MII) dan Imatur (MI) Pada Pasien Bayi Tabung","authors":"Ni Putu Eny Sulistya Dewi, N. I. Wiratmini, J. Sudiman","doi":"10.24843/metamorfosa.2022.v09.i02.p01","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p01","url":null,"abstract":"Oocyte developmental competence is one of the determining factors that in?uence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis of oocyte cumulus cells (CCs) can be used as a method to predict oocyte competence and viability that could potentially function as molecular predictors for IVF program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 and BAX) that are expressed in human CCs between mature (MII) and immature (MI) oocytes. This research is an analytic observational study. Twelve samples of CCs from mature (MII) and immature (MI) oocytes were collected after ovum pick up from patients undergoing ICSI. CCs samples were isolated using an ultrasonicator and protein expressions were quantified using ELISA method. The Mann-Whitney test was used to compare the protein expressions from CCs between mature and immature oocytes. The results showed HAS2, PTX3 and BCL2 proteins expressions was increase in CCs from mature oocytes but had no significant effect compared to CCs from immature oocytes, however, BAX protein expression was significantly higher (P<0.05) in CCs from immature oocytes which CCs from mature oocytes. This present study shows that BAX proteins could be used as markers for oocyte quality and maturation. \u0000Key words: ELISA, maturation, proteins marker, cumulus cells (CCs)","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48041837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Iniobong, J. Adedipe, A. Yakub, K. Balogun, B. Bello, O. Abiodun
{"title":"Phytoplankton Composition, Abundance and Diversity in Some Selected Parts of Lagos Lagoon","authors":"A. Iniobong, J. Adedipe, A. Yakub, K. Balogun, B. Bello, O. Abiodun","doi":"10.3923/jbs.2022.80.90","DOIUrl":"https://doi.org/10.3923/jbs.2022.80.90","url":null,"abstract":"","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87368402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: E-cigarettes (e-cig) produce smoke with high levels of free oxidants, which can be eliminated by endogenous antioxidants such as glutathione peroxidase (GPx) and catalase (CAT) in the human body. The GPx catabolizes hydrogen peroxide (H2O2), while catalase counteracts free radicals by catalyzing H2O2 into H2O and O2. However, oxidative stress is shown in the imbalance condition between free oxidants and endogenous antioxidants. The effect of e-cigarette smoking on the human body has not been discovered. Therefore, this research aimed to analyze the levels of GPx and CAT in the rat blood exposed to e-cig. Materials and Methods: The exposure to smoke with nicotine 3, 6 and 9 mg was applied to 30 rats divided into five groups for 30 days. The analysis results by one-way ANOVA showed significant differences in the GPx and CAT levels between the control and treatment groups. Nicotine at 9 mg gave the GPx level at 40.25±2.03 U mgG1 and CAT at 2.46±0.50 nmol mLG1. Results: The results indicated that e-cigarette smoke reduced the levels of endogenous antioxidants in rat blood. Moreover, the body weight of rats increased and decreased due to the unstable metabolic rate. Conclusion: Exposure to e-cigarette smoke has an effect on reducing glutathione peroxidase (GPx) and catalase (CAT) levels in rat blood.
背景与目的:电子烟(e- cigg)产生的烟雾中含有高浓度的游离氧化剂，可被人体内的谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)等内源性抗氧化剂消除。GPx分解过氧化氢(H2O2)，而过氧化氢酶通过将H2O2催化成H2O和O2来抵消自由基。然而，氧化应激表现为游离氧化剂与内源性抗氧化剂之间的失衡状态。电子烟对人体的影响尚未被发现。因此，本研究旨在分析暴露于电子烟的大鼠血液中GPx和CAT的水平。材料与方法:将30只大鼠分为5组，分别给予含尼古丁3、6、9 mg的烟雾暴露30 d。单因素方差分析结果显示，对照组和治疗组之间GPx和CAT水平存在显著差异。9 mg尼古丁使GPx水平为40.25±2.03 U mgG1, CAT水平为2.46±0.50 nmol mLG1。结果:电子烟烟雾降低了大鼠血液中内源性抗氧化剂的水平。此外，由于代谢率不稳定，大鼠的体重有增有减。结论:电子烟烟雾可降低大鼠血液中谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)水平。
{"title":"Analysis of Endogenous Antioxidants of Rat Blood Exposed to E-Cigarette","authors":"L. Lisdiana, N. Harini, Priyantini Widiyaning","doi":"10.3923/jbs.2022.91.98","DOIUrl":"https://doi.org/10.3923/jbs.2022.91.98","url":null,"abstract":"Background and Objective: E-cigarettes (e-cig) produce smoke with high levels of free oxidants, which can be eliminated by endogenous antioxidants such as glutathione peroxidase (GPx) and catalase (CAT) in the human body. The GPx catabolizes hydrogen peroxide (H2O2), while catalase counteracts free radicals by catalyzing H2O2 into H2O and O2. However, oxidative stress is shown in the imbalance condition between free oxidants and endogenous antioxidants. The effect of e-cigarette smoking on the human body has not been discovered. Therefore, this research aimed to analyze the levels of GPx and CAT in the rat blood exposed to e-cig. Materials and Methods: The exposure to smoke with nicotine 3, 6 and 9 mg was applied to 30 rats divided into five groups for 30 days. The analysis results by one-way ANOVA showed significant differences in the GPx and CAT levels between the control and treatment groups. Nicotine at 9 mg gave the GPx level at 40.25±2.03 U mgG1 and CAT at 2.46±0.50 nmol mLG1. Results: The results indicated that e-cigarette smoke reduced the levels of endogenous antioxidants in rat blood. Moreover, the body weight of rats increased and decreased due to the unstable metabolic rate. Conclusion: Exposure to e-cigarette smoke has an effect on reducing glutathione peroxidase (GPx) and catalase (CAT) levels in rat blood.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85531024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.24843/metamorfosa.2022.v09.i01.p18
Retno Kawuri, I. Darmayasa
Pencemaranan limbah minyak dan lemak ke lingkungan perairan yang berasal dari pengolahan makanan industri, restoran dan dapur atau oleh tumpahan minyak yang tidak disengaja saat ini semakin banyak ditemukan. Biodegradasi menggunakan konsorsium bakteri dapat menjadi salah satu cara yang efektif untuk mengatasi hal tersebut. Tujuan penelitian untuk mengisolasi dan mengidentifikasi strain bakteri dari limbah minyak dan lemak yang mampu melakukan biodegradasi serta potensi dari bakteri pendegradasi dalam mendegradasi minyak dan lemak secara invitro. Sampel diambil dari limbah minyak dan lemak. Pada seluruh sampel dilakukan teknik pengayaan diikuti dengan isolasi strain bakteri untuk menentukan strain mana yang mampu mendegradasi minyak dan lemak secara in vitro. Identifikasi bakteri menggunakan Identifikasi kit BBL Crystal System. Penghitungan total bakteri dilakukan dengan menggunakan metode pengenceran. Hasil menunjukan ditemukan 3 strain bakteri pendegradasi minyak dan lemak yaitu Bacillus licheneformis , Bacillus coagulan dan Psedomonas diminuta. Konsorsium dari ketiga jenis bakteri tersebut mampu mendegradasi limbah minyak dan lemak dengan total bakteri 624x105 CFU/ml hingga 816x105 CFU/ml dengan kontrol tanpa penambahan konsorsium bakteri yaitu sebesar 56x101 CFU/ml. Total bakteri pada berbagai limbah perlakuan dengan konsorsium bakteri berbahan dasar molase pada uji secara invitro menunjukan kemampuan hidup dan berkembang dari konsorsium bakteri yang cukup tinggi yaitu 292x104 CFU/ml hingga 904x104 CFU/ml.. Bakteri yang ditemukan mempunyai kemampuan mendegradasi minyak dan lemak yang bekerja secara sinergistik. Manfaat dari penelitian ini bakteri yang telah ditemukan dapat digunakan sebagai starter pengolahan limbah lemak dan minyak di lingkungan yang tercemar limbah lemak dan minyak domestik. Kata kunci: konsorsium bakteri, biodegradasi, limbah minyak dan lemak
{"title":"Potensi Bakteri Sebagai Biodegradasi Lemak Dan Minyak Pada Lingkungan Yang Tercemar Limbah Domestik","authors":"Retno Kawuri, I. Darmayasa","doi":"10.24843/metamorfosa.2022.v09.i01.p18","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i01.p18","url":null,"abstract":"Pencemaranan limbah minyak dan lemak ke lingkungan perairan yang berasal dari pengolahan makanan industri, restoran dan dapur atau oleh tumpahan minyak yang tidak disengaja saat ini semakin banyak ditemukan. Biodegradasi menggunakan konsorsium bakteri dapat menjadi salah satu cara yang efektif untuk mengatasi hal tersebut. Tujuan penelitian untuk mengisolasi dan mengidentifikasi strain bakteri dari limbah minyak dan lemak yang mampu melakukan biodegradasi serta potensi dari bakteri pendegradasi dalam mendegradasi minyak dan lemak secara invitro. Sampel diambil dari limbah minyak dan lemak. Pada seluruh sampel dilakukan teknik pengayaan diikuti dengan isolasi strain bakteri untuk menentukan strain mana yang mampu mendegradasi minyak dan lemak secara in vitro. Identifikasi bakteri menggunakan Identifikasi kit BBL Crystal System. Penghitungan total bakteri dilakukan dengan menggunakan metode pengenceran. Hasil menunjukan ditemukan 3 strain bakteri pendegradasi minyak dan lemak yaitu Bacillus licheneformis , Bacillus coagulan dan Psedomonas diminuta. Konsorsium dari ketiga jenis bakteri tersebut mampu mendegradasi limbah minyak dan lemak dengan total bakteri 624x105 CFU/ml hingga 816x105 CFU/ml dengan kontrol tanpa penambahan konsorsium bakteri yaitu sebesar 56x101 CFU/ml. Total bakteri pada berbagai limbah perlakuan dengan konsorsium bakteri berbahan dasar molase pada uji secara invitro menunjukan kemampuan hidup dan berkembang dari konsorsium bakteri yang cukup tinggi yaitu 292x104 CFU/ml hingga 904x104 CFU/ml.. Bakteri yang ditemukan mempunyai kemampuan mendegradasi minyak dan lemak yang bekerja secara sinergistik. Manfaat dari penelitian ini bakteri yang telah ditemukan dapat digunakan sebagai starter pengolahan limbah lemak dan minyak di lingkungan yang tercemar limbah lemak dan minyak domestik. \u0000Kata kunci: konsorsium bakteri, biodegradasi, limbah minyak dan lemak \u0000 ","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41321300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-17DOI: 10.24843/metamorfosa.2022.v09.i01.p22
Ida Bagus Made Bramasta Wirabumi, Eniek Kriswiyanti, A. Darmadi
The aim of this research are to know 1)Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 2) Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 3) Index of Cultural Significance (ICS) Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area. This research is conducted by collecting Ceremonial Plant of Ngaben exist on every quadrat level of the Mandala. The ploting method being used in this research is stratified random sampling with type of plot is single square plot. The data were analyzed using Shanon – Wienner Index, Simpson Index and ICS Turner Index. The result of the research are as follows: 1) Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area considerably high with score of 2,5302; 2) Score of Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area is also high, which is 0,994214; 3) Higher score of ICS were granted by Kelapa (Cocos nucifera) with score 232; Cabai (Capsicum frustecens) with score of 80; Biu (Musa paradisiaca) with score of 70
{"title":"Analisis Keanekaragaman, Indeks Nilai Penting dan Index of Cultural Significance Tumbuhan Upacara Ngaben Berdasarkan Tri Mandala di Desa Penglipuran, Bali.","authors":"Ida Bagus Made Bramasta Wirabumi, Eniek Kriswiyanti, A. Darmadi","doi":"10.24843/metamorfosa.2022.v09.i01.p22","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i01.p22","url":null,"abstract":"The aim of this research are to know 1)Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 2) Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 3) Index of Cultural Significance (ICS) Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area. This research is conducted by collecting Ceremonial Plant of Ngaben exist on every quadrat level of the Mandala. The ploting method being used in this research is stratified random sampling with type of plot is single square plot. The data were analyzed using Shanon – Wienner Index, Simpson Index and ICS Turner Index. The result of the research are as follows: 1) Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area considerably high with score of 2,5302; 2) Score of Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area is also high, which is 0,994214; 3) Higher score of ICS were granted by Kelapa (Cocos nucifera) with score 232; Cabai (Capsicum frustecens) with score of 80; Biu (Musa paradisiaca) with score of 70","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49024010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-15DOI: 10.24843/metamorfosa.2022.v09.i01.p20
Puji Wahyu Lestari, M. Defiani, M. Kosmiatin
Jeruk siam Kintamani disukai oleh konsumen karena memiliki rasa manis, harum, dan daging buahnya lunak. Namun jeruk ini memiliki biji relatif banyak dan warna kulit kurang menarik sehingga kalah bersaing dengan jeruk yang diproduksi oleh negara lain. Alternatif untuk meningkatkan kualitas buah jeruk adalah melalui induksi mutasi in vitro menggunakan sinar gamma. Tunas putative mutan jeruk siam diperbanyak secara in vitro sehingga diperoleh duplikat dari masing-masing mutan. Penggunaan jenis media dan zat pengatur tumbuh pada konsentrasi yang tepat merupakan salah satu kunci keberhasilan dalam kultur jaringan. Tujuan penelitian ini adalah untuk memacu multiplikasi tunas dari eksplan buku. Bahan yang digunakan yaitu plantlet jeruk siam Kintamani hasil iradiasi sinar gamma dengan dosis 0, 4,5, 5, dan 5,5 Gy. Penelitian multiplikasi tunas secara in vitro menggunakan eksplan buku dengan perlakuan 7 jenis media. Hasil penelitian menunjukkan bahwa pada perlakuan media MS VMW + BAP2 + GA2 mampu memperbanyak jumlah tunas, sedangkan media MS VMW mampu meningkatkan jumlah daun dan perbanyakan jumlah akar. Kata kunci : putative mutan, karakterisasi, multiplikasi, mutasi
{"title":"Perbanyakan Klonal Galur Jeruk Siam Kintamani (Citrus nobilis Lour) Hasil Iradiasi Sinar Gamma","authors":"Puji Wahyu Lestari, M. Defiani, M. Kosmiatin","doi":"10.24843/metamorfosa.2022.v09.i01.p20","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i01.p20","url":null,"abstract":"Jeruk siam Kintamani disukai oleh konsumen karena memiliki rasa manis, harum, dan daging buahnya lunak. Namun jeruk ini memiliki biji relatif banyak dan warna kulit kurang menarik sehingga kalah bersaing dengan jeruk yang diproduksi oleh negara lain. Alternatif untuk meningkatkan kualitas buah jeruk adalah melalui induksi mutasi in vitro menggunakan sinar gamma. Tunas putative mutan jeruk siam diperbanyak secara in vitro sehingga diperoleh duplikat dari masing-masing mutan. Penggunaan jenis media dan zat pengatur tumbuh pada konsentrasi yang tepat merupakan salah satu kunci keberhasilan dalam kultur jaringan. Tujuan penelitian ini adalah untuk memacu multiplikasi tunas dari eksplan buku. Bahan yang digunakan yaitu plantlet jeruk siam Kintamani hasil iradiasi sinar gamma dengan dosis 0, 4,5, 5, dan 5,5 Gy. Penelitian multiplikasi tunas secara in vitro menggunakan eksplan buku dengan perlakuan 7 jenis media. Hasil penelitian menunjukkan bahwa pada perlakuan media MS VMW + BAP2 + GA2 mampu memperbanyak jumlah tunas, sedangkan media MS VMW mampu meningkatkan jumlah daun dan perbanyakan jumlah akar. \u0000Kata kunci : putative mutan, karakterisasi, multiplikasi, mutasi","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46013020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-15DOI: 10.24843/metamorfosa.2022.v09.i01.p21
Riska Rismawati, Diana Hernawati, D. M. Chaidir
Green turtle (Chelonia mydas) is one of the protected animals which has a very important role for marine life. Pangumbahan Beach is one of the green turtle nesting habitats in Indonesia which consists of six landing stations with a length of 2.3 km. The success of nesting green turtles is greatly influenced by the conditions of the habitat and the activities contained therein. The purpose of this study is to determine the frequency of landing of green turtles at each station and to describe the green turtle nesting activity on Pangumbahan beach. The method used was observation, with the determination of the research point using purposive sampling. The green turtle egg laying activity consists of ten stages starting from appearing on the shoreline, then crawling up to the coast, looking for the right laying of the appropriate eggs, making a body fit, compacting the body hole, making small holes to place eggs, the process of laying eggs, then after laying its eggs, the green turtle closes the hole it made and makes a camouflage hole before returning to the sea. The number of green turtles that landed during March was 47. The results of the analysis of the highest green turtle landing frequency were at station 2 and station 3, namely 35.48%, which was included rare category. Then the station 1 frequency was 19.35%, the station 4 frequency was 16.13%, the station 5 frequency was 22.58% and the station 6 frequency was 0% included in the very rare category.The result of this study can be used as additional information for the management of green turtle (Chelonia mydas) conservation.
{"title":"Aktivitas Bertelur dan Frekuensi Pendaratan Penyu Hijau (Chelonia mydas) di Pantai Pangumbahan Sukabumi","authors":"Riska Rismawati, Diana Hernawati, D. M. Chaidir","doi":"10.24843/metamorfosa.2022.v09.i01.p21","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i01.p21","url":null,"abstract":"Green turtle (Chelonia mydas) is one of the protected animals which has a very important role for marine life. Pangumbahan Beach is one of the green turtle nesting habitats in Indonesia which consists of six landing stations with a length of 2.3 km. The success of nesting green turtles is greatly influenced by the conditions of the habitat and the activities contained therein. The purpose of this study is to determine the frequency of landing of green turtles at each station and to describe the green turtle nesting activity on Pangumbahan beach. The method used was observation, with the determination of the research point using purposive sampling. The green turtle egg laying activity consists of ten stages starting from appearing on the shoreline, then crawling up to the coast, looking for the right laying of the appropriate eggs, making a body fit, compacting the body hole, making small holes to place eggs, the process of laying eggs, then after laying its eggs, the green turtle closes the hole it made and makes a camouflage hole before returning to the sea. The number of green turtles that landed during March was 47. The results of the analysis of the highest green turtle landing frequency were at station 2 and station 3, namely 35.48%, which was included rare category. Then the station 1 frequency was 19.35%, the station 4 frequency was 16.13%, the station 5 frequency was 22.58% and the station 6 frequency was 0% included in the very rare category.The result of this study can be used as additional information for the management of green turtle (Chelonia mydas) conservation.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47456911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kompatibilitas suatu persilangan ditandai dengan terbentuknya buah. Kompatibilitas suatu persilangan interspesies atau persilangan antara spesies anggrek yang berbeda digunakan untuk meningkatkan keragaman. Untuk membandingkan dan mengetahui daya kompatibilitas perlu dilakukan persilangan secara bolak-balik (resiprok). Tujuan dari penelitian ini adalah untuk mengetahui kompatibel persilangan interspesies dan resiprok anggrek Dendrobium dan mengamati pertumbuhan buah hasil persilangan. Prosedur penelitian yaitu menyilangkan pollen pada satu spesies bunga anggrek ke stigma spesies anggrek lain, kemudian variabel yang diamati adalah saat terbentuknya buah (hari setelah penyerbukan/hsp), hari layu sepal dan petal (hsp), panjang dan diameter buah (cm) selama dua bulan pengamatan, kompatibilitas persilangan, dan buah/bunga rontok. Data yang diperoleh dianalisis menggunakan analisis deskriptif kuantitatif. Hasil dari penelitian ini diketahui hari layu sepal dan petal sangat bervariasi dari 10-22 hari. Terbentuknya buah dari hari ke-7-16. Hari terbentuknya buah sangat bervariasi tergantung pada spesies yang disilangkan. Bentuk buah ditentukan oleh indukan betina, sedangkan ukuran buah bergantung pada nutrisi yang terdapat pada tanaman. Kompatibilitas persilangan ini adalah 75% dan dapat dikatakan bahwa indukan yang digunakan kompatibel. Kata kunci: Persilangan, Interspesies, Resiprok, Dendrobium, Kompatibilitas.
{"title":"Uji Kompatibilitas Persilangan Interspesies dan Resiprok Anggrek Dendrobium","authors":"Reza Priski Dwi Jayanti, Tintrim Rahayu, Gatra Ervi Jayanti","doi":"10.24843/metamorfosa.2022.v09.i01.p19","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i01.p19","url":null,"abstract":"Kompatibilitas suatu persilangan ditandai dengan terbentuknya buah. Kompatibilitas suatu persilangan interspesies atau persilangan antara spesies anggrek yang berbeda digunakan untuk meningkatkan keragaman. Untuk membandingkan dan mengetahui daya kompatibilitas perlu dilakukan persilangan secara bolak-balik (resiprok). Tujuan dari penelitian ini adalah untuk mengetahui kompatibel persilangan interspesies dan resiprok anggrek Dendrobium dan mengamati pertumbuhan buah hasil persilangan. Prosedur penelitian yaitu menyilangkan pollen pada satu spesies bunga anggrek ke stigma spesies anggrek lain, kemudian variabel yang diamati adalah saat terbentuknya buah (hari setelah penyerbukan/hsp), hari layu sepal dan petal (hsp), panjang dan diameter buah (cm) selama dua bulan pengamatan, kompatibilitas persilangan, dan buah/bunga rontok. Data yang diperoleh dianalisis menggunakan analisis deskriptif kuantitatif. Hasil dari penelitian ini diketahui hari layu sepal dan petal sangat bervariasi dari 10-22 hari. Terbentuknya buah dari hari ke-7-16. Hari terbentuknya buah sangat bervariasi tergantung pada spesies yang disilangkan. Bentuk buah ditentukan oleh indukan betina, sedangkan ukuran buah bergantung pada nutrisi yang terdapat pada tanaman. Kompatibilitas persilangan ini adalah 75% dan dapat dikatakan bahwa indukan yang digunakan kompatibel. \u0000 \u0000Kata kunci: Persilangan, Interspesies, Resiprok, Dendrobium, Kompatibilitas.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45353737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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