Pub Date : 2022-10-27DOI: 10.24843/metamorfosa.2022.v09.i02.p16
Winda Tul Habibah, Tintrim Rahayu, M. Ramadhan
Koro Benguk (Mucuna pruriens D.C.) is a plant that produces medicinal ingredients containing secondary metabolites, one of which is L-DOPA. This compound can be used to treat nervous disorders, treat snake venom, increase body weight and muscle strength, and as an anthelmintic in humans. This study aims to analyze the Benguk Koro Bean (Mucuna pruriens D.C.) In Silico and detect the presence of L-DOPA compounds in the Benguk Koro Bean (Mucuna pruriens D.C.) species using UV-VIS Spectrometry. The methods used are the description method and the data processed descriptively qualitatively. The results showed that in In Silico analysis it was found that the Benguk Koro Bean (Mucuna pruriens D.C.) species in the seeds and leaves contained L-DOPA compounds and had a C9H11NO4 molecular structure, while for the detection of L-DOPA compounds using UV-VIS spectrometry it was detected that Koro Benguk Bean (Mucuna pruriens D.C.) contains L-DOPA with a wavelength of 462 nm with absorbance in seeds and leaves of 2,210 and 1,171, respectively. The conclusion of this study is that with 20 grams of koro benguk seeds it produces 1.105 ppm, while for 2 grams of koro benguk leaves it produces 0.5855 ppm.
{"title":"Analisis In Silico dan Kuantitatif Senyawa Metabolit Sekunder Senyawa L-DOPA Pada Ekstrak Biji Dan Daun Kacang Koro Benguk (Mucuna pruriens D.C.) menggunakan metode Spektrometri UV-VIS","authors":"Winda Tul Habibah, Tintrim Rahayu, M. Ramadhan","doi":"10.24843/metamorfosa.2022.v09.i02.p16","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p16","url":null,"abstract":"Koro Benguk (Mucuna pruriens D.C.) is a plant that produces medicinal ingredients containing secondary metabolites, one of which is L-DOPA. This compound can be used to treat nervous disorders, treat snake venom, increase body weight and muscle strength, and as an anthelmintic in humans. This study aims to analyze the Benguk Koro Bean (Mucuna pruriens D.C.) In Silico and detect the presence of L-DOPA compounds in the Benguk Koro Bean (Mucuna pruriens D.C.) species using UV-VIS Spectrometry. The methods used are the description method and the data processed descriptively qualitatively. The results showed that in In Silico analysis it was found that the Benguk Koro Bean (Mucuna pruriens D.C.) species in the seeds and leaves contained L-DOPA compounds and had a C9H11NO4 molecular structure, while for the detection of L-DOPA compounds using UV-VIS spectrometry it was detected that Koro Benguk Bean (Mucuna pruriens D.C.) contains L-DOPA with a wavelength of 462 nm with absorbance in seeds and leaves of 2,210 and 1,171, respectively. The conclusion of this study is that with 20 grams of koro benguk seeds it produces 1.105 ppm, while for 2 grams of koro benguk leaves it produces 0.5855 ppm.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41857148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.24843/metamorfosa.2022.v09.i02.p13
Anak Agung Gede Agung Putra Dalem, Job Nico Subagyo, N. M. Suartini
INTISARI Penelitian tentang “Biodiversitas Capung di Desa Petulu, Kecamatan Ubud, Kabupaten Gianyar, Bali” dilaksanakan pada bulan Mei-Oktober 2020. Tujuan penelitian adalah untuk mengetahui jenis-jenis capung dan biodiversitas atau keanekaragaman capung yang ditemukan di Desa Petulu, Kecamatan Ubud, Provinsi Bali, melalui eksplorasi. Sampel diambil pada beberapa lokasi mewakili habitat yang berbeda, diantaranya sawah, pemukiman, dan tegalan. Capung yang ditemukan di lapangan (jika memungkinkan) ditangkap dengan insect net dan dibawa ke laboratorium di Program Studi Biologi-FMIPA Unud untuk identifikasi jenisnya. Hasil pengamatan dicocokkan dengan panduan antara lain Wahyu Sigit Rhd (2013), Lilies S. (1992), dan CSIRO (1991). Data capung dicatat berupa jenis dan jumlahnya (relative), kemudian hasilnya ditampilkan dalam tabel. Data disampaikan antara lain menyangkut status (dilindungi atau tidak), dan indeks biodiversitasnya. Indeks biodiversitas dihitung dengan indeks Shannon Wiener. Hasil penelitian menunjukkan bahwa telah berhasil diidentifikasi 12 species capung dalam penelitian ini, yaitu: Crocothemis servilia, Pantala flavescens, Orthetrum sabina, Neurothemis terminata, Diplacodes trivialis, Potamarcha congener, Neurothemis ramburii, Orthetrum chrysis, Ischnura senegalensis, Agriocnemis femina, Agrionoptera insignis, dan Agriocnemis pygmaea.Semua jenis capung yang ditemukan di wilayah studi termasuk capung yang tidak dilindungi. Indeks biodiversitas (H’) capung di Desa Petulu adalah sebesar 1,63, yang mengindikasikan kondisi struktur komunitas capung di Desa Petulu termasuk stabil dalam kategori sedang. Kata kunci: Bali, biodiversitas, capung, identifikasi, Petulu
{"title":"Biodiversitas Capung Di Desa Petulu, Kecamatan Ubud, Kabupaten Gianyar, Bali","authors":"Anak Agung Gede Agung Putra Dalem, Job Nico Subagyo, N. M. Suartini","doi":"10.24843/metamorfosa.2022.v09.i02.p13","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p13","url":null,"abstract":"INTISARI \u0000Penelitian tentang “Biodiversitas Capung di Desa Petulu, Kecamatan Ubud, Kabupaten Gianyar, Bali” dilaksanakan pada bulan Mei-Oktober 2020. Tujuan penelitian adalah untuk mengetahui jenis-jenis capung dan biodiversitas atau keanekaragaman capung yang ditemukan di Desa Petulu, Kecamatan Ubud, Provinsi Bali, melalui eksplorasi. Sampel diambil pada beberapa lokasi mewakili habitat yang berbeda, diantaranya sawah, pemukiman, dan tegalan. Capung yang ditemukan di lapangan (jika memungkinkan) ditangkap dengan insect net dan dibawa ke laboratorium di Program Studi Biologi-FMIPA Unud untuk identifikasi jenisnya. Hasil pengamatan dicocokkan dengan panduan antara lain Wahyu Sigit Rhd (2013), Lilies S. (1992), dan CSIRO (1991). Data capung dicatat berupa jenis dan jumlahnya (relative), kemudian hasilnya ditampilkan dalam tabel. Data disampaikan antara lain menyangkut status (dilindungi atau tidak), dan indeks biodiversitasnya. Indeks biodiversitas dihitung dengan indeks Shannon Wiener. Hasil penelitian menunjukkan bahwa telah berhasil diidentifikasi 12 species capung dalam penelitian ini, yaitu: Crocothemis servilia, Pantala flavescens, Orthetrum sabina, Neurothemis terminata, Diplacodes trivialis, Potamarcha congener, Neurothemis ramburii, Orthetrum chrysis, Ischnura senegalensis, Agriocnemis femina, Agrionoptera insignis, dan Agriocnemis pygmaea.Semua jenis capung yang ditemukan di wilayah studi termasuk capung yang tidak dilindungi. Indeks biodiversitas (H’) capung di Desa Petulu adalah sebesar 1,63, yang mengindikasikan kondisi struktur komunitas capung di Desa Petulu termasuk stabil dalam kategori sedang. \u0000Kata kunci: Bali, biodiversitas, capung, identifikasi, Petulu","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42465871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.24843/metamorfosa.2022.v09.i02.p15
Luh Gede Artha Saridewi Wijaya, N. L. Arpiwi, Ida Ayu Astarini
ABSTRACT A research was carried out to determine the yield, content of constituent compounds, and analysis of the quality of clove leaf essential oil (Syzygium aromaticum L.) at different altitudes. The study used dry clove leaves obtained from Buleleng Regency, Bali Province with an altitude of 300 meters above sea level (masl), 600 masl, and 900 masl. Extraction of essential oils was carried out by steam distillation. The essential oil obtained was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and the quality was tested using the quality requirements of SNI 06-2387-2006. Parameters observed were yield, identification of constituent compounds, analysis of essential oil quality including color, odor, specific gravity, and refractive index, solubility in alcohol, optical rotation, total eugenol and caryophyllene. The results showed that clove leaf essential oil in the lowlands (300 masl) had a higher yield of 2.05% and more types of active compounds than in the optimal altitude (600 masl) which was 1.45% and the highlands (900 masl) was 1.85%. The main compounds that make up clove leaf essential oil were eugenol, caryophyllene, and humulene. Other compounds were longifoline, nootkatone, naphthalene, citronellal, and cyclohexene. The clove leaf essential oil from the three heights met the quality requirements of SNI 06-2387-2006. Keywords: Essential oil, cloves, eugenol, GC-MS, secondary metabolites
{"title":"Analisis Minyak Atsiri Daun Cengkeh (Syzygium aromaticum L.) Dari Tempat Tumbuh Dengan Ketinggian Yang Berbeda","authors":"Luh Gede Artha Saridewi Wijaya, N. L. Arpiwi, Ida Ayu Astarini","doi":"10.24843/metamorfosa.2022.v09.i02.p15","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p15","url":null,"abstract":"ABSTRACT \u0000A research was carried out to determine the yield, content of constituent compounds, and analysis of the quality of clove leaf essential oil (Syzygium aromaticum L.) at different altitudes. The study used dry clove leaves obtained from Buleleng Regency, Bali Province with an altitude of 300 meters above sea level (masl), 600 masl, and 900 masl. Extraction of essential oils was carried out by steam distillation. The essential oil obtained was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and the quality was tested using the quality requirements of SNI 06-2387-2006. Parameters observed were yield, identification of constituent compounds, analysis of essential oil quality including color, odor, specific gravity, and refractive index, solubility in alcohol, optical rotation, total eugenol and caryophyllene. The results showed that clove leaf essential oil in the lowlands (300 masl) had a higher yield of 2.05% and more types of active compounds than in the optimal altitude (600 masl) which was 1.45% and the highlands (900 masl) was 1.85%. The main compounds that make up clove leaf essential oil were eugenol, caryophyllene, and humulene. Other compounds were longifoline, nootkatone, naphthalene, citronellal, and cyclohexene. The clove leaf essential oil from the three heights met the quality requirements of SNI 06-2387-2006. \u0000Keywords: Essential oil, cloves, eugenol, GC-MS, secondary metabolites","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42695198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.24843/metamorfosa.2022.v09.i02.p14
Rendy Sinaga, Ida Bagus Wayan Gunam, N. S. Antara
INTISARI Penelitian ini bertujuan untuk mengetahui konsentrasi substrat selulosa kasar brangkasan jagung dan waktu sakarifikasi terbaik untuk menghasilkan kadar glukosa yang tinggi. Selulosa yang terkandung pada brangkasan jagung memiliki potensi menjadi glukosa melalu proses sakarifikasi enzimatis menggunakan enzim selulase. Produksi glukosa pada konsentrasi substrat dan waktu sakarifikasi yang berbeda menggunakan Rancangan Acak Kelompok (RAK) faktorial yang terdiri dari dua faktor. Faktor pertama adalah konsentrasi substrat yang terdiri dari 4 taraf yaitu 2%, 3%, 4%, dan 5% (b/v). Faktor kedua adalah waktu sakarifikasi yang terdiri dari 4 taraf yaitu 24 jam, 48 jam, 72 jam, dan 96 jam. Variabel yang diamati meliputi kadar glukosa, residu selulosa, pH, dan total padatan terlarut. Hasil penelitian menunjukkan bahwa pada konsentrasi substrat 5% dan waktu sakarifikasi 96 jam merupakan perlakuan terbaik untuk menghasilkan kadar glukosa maksimum sebesar 0,3003 mg/mL, residu selulosa minimum setelah sakarifikasi sebesar 17,75%, pH setelah sakarifikasi sebesar 6,1 dan total padatan terlarut tertinggi setelah sakarifikasi sebesar 2,7°Brix. Kata kunci : enzimatis, glukosa, sakarifikasi, selulosa kasar brangkasan jagung
{"title":"Produksi Glukosa dengan Substrat Selulosa Kasar Brangkasan Jagung Menggunakan Enzim Selulase dari Isolat B2S8","authors":"Rendy Sinaga, Ida Bagus Wayan Gunam, N. S. Antara","doi":"10.24843/metamorfosa.2022.v09.i02.p14","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p14","url":null,"abstract":"INTISARI \u0000Penelitian ini bertujuan untuk mengetahui konsentrasi substrat selulosa kasar brangkasan jagung dan waktu sakarifikasi terbaik untuk menghasilkan kadar glukosa yang tinggi. Selulosa yang terkandung pada brangkasan jagung memiliki potensi menjadi glukosa melalu proses sakarifikasi enzimatis menggunakan enzim selulase. Produksi glukosa pada konsentrasi substrat dan waktu sakarifikasi yang berbeda menggunakan Rancangan Acak Kelompok (RAK) faktorial yang terdiri dari dua faktor. Faktor pertama adalah konsentrasi substrat yang terdiri dari 4 taraf yaitu 2%, 3%, 4%, dan 5% (b/v). Faktor kedua adalah waktu sakarifikasi yang terdiri dari 4 taraf yaitu 24 jam, 48 jam, 72 jam, dan 96 jam. Variabel yang diamati meliputi kadar glukosa, residu selulosa, pH, dan total padatan terlarut. Hasil penelitian menunjukkan bahwa pada konsentrasi substrat 5% dan waktu sakarifikasi 96 jam merupakan perlakuan terbaik untuk menghasilkan kadar glukosa maksimum sebesar 0,3003 mg/mL, residu selulosa minimum setelah sakarifikasi sebesar 17,75%, pH setelah sakarifikasi sebesar 6,1 dan total padatan terlarut tertinggi setelah sakarifikasi sebesar 2,7°Brix. \u0000 \u0000Kata kunci : enzimatis, glukosa, sakarifikasi, selulosa kasar brangkasan jagung","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48569020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-19DOI: 10.24843/metamorfosa.2022.v09.i02.p10
Wan Fadilah, Rasyida ., Ulfa Mayasari
Penelitian ini bertujuan untuk mengetahui adanya bakteri heterotrofik pada kawasan perairan pantai Indah Kalangan, Tapanuli Tengah serta genus bakteri heterotrofik apa saja yang ditemukan pada kawasan perairan pantai Indah Kalangan, Tapanuli Tengah. Prosedur kerja dalam penelitian ini dengan karakterisasi morfologi bakteri, pewarnaan gram dan juga karakterisasi berdasarkan uji biokimia. Metode deskriptif akan digunakan dalam proses analisis data menggunakan panduan buku Bergey’s Manual of Determinative Bacteriology 8th Edition, Bergey’s Manual Of Systematic Bacteriology 2nd Edition dan jurnal. Hasil dari penelitian ini diperoleh 16 isolat bakteri heterotrofik, dimana 13 isolat bakteri tergolong genus Bacillus dan 3 isolat bakteri tergolong genus Vibrio. Dari hasil penelitian menunjukkan bahwa perairan pantai Indah Kalangan terdapat bakteri heterotrofik dengan genus Bacillus dan genus Vibrio. Kata Kunci: Bakteri Heterotrofik, Bacillus, Vibrio, Pantai Indah Kalangan
{"title":"Isolasi Dan Karakterisasi Bakteri Heterotrofik Pada Kawasan Perairan Pantai Indah Kalangan, Tapanuli Tengah","authors":"Wan Fadilah, Rasyida ., Ulfa Mayasari","doi":"10.24843/metamorfosa.2022.v09.i02.p10","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p10","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui adanya bakteri heterotrofik pada kawasan perairan pantai Indah Kalangan, Tapanuli Tengah serta genus bakteri heterotrofik apa saja yang ditemukan pada kawasan perairan pantai Indah Kalangan, Tapanuli Tengah. Prosedur kerja dalam penelitian ini dengan karakterisasi morfologi bakteri, pewarnaan gram dan juga karakterisasi berdasarkan uji biokimia. Metode deskriptif akan digunakan dalam proses analisis data menggunakan panduan buku Bergey’s Manual of Determinative Bacteriology 8th Edition, Bergey’s Manual Of Systematic Bacteriology 2nd Edition dan jurnal. Hasil dari penelitian ini diperoleh 16 isolat bakteri heterotrofik, dimana 13 isolat bakteri tergolong genus Bacillus dan 3 isolat bakteri tergolong genus Vibrio. Dari hasil penelitian menunjukkan bahwa perairan pantai Indah Kalangan terdapat bakteri heterotrofik dengan genus Bacillus dan genus Vibrio. \u0000 \u0000Kata Kunci: Bakteri Heterotrofik, Bacillus, Vibrio, Pantai Indah Kalangan","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45650271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-19DOI: 10.24843/metamorfosa.2022.v09.i02.p11
Putu Susilawati, B. K. Satriyasa, I. G. A. Widianti
Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) dapat menurunkan kualitas spermatozoa mencit (Mus musculus) dewasa muda sebagai bahan kontrasepsi pria alami. Penelitian ini merupakan penelitian eksperimental dengan menggunakan rancangan penelitian post-test only control group design. Sampel penelitian ini terdiri dari 36 ekor mencit (Mus musculus) jantan galur Swiss Webster usia 2-3 bulan dengan berat badan 20-30 gram yang dibagi menjadi dua kelompok secara acak. Kelompok kontrol (P0) diberikan CMC Na 0,3% sebanyak 0,5 ml dan kelompok perlakuan (P1) diberikan ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) 0,4 mg/gram BB sebanyak 0,5 ml selama 36 hari per oral. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) dapat menurunkan kualitas (konsentrasi, motilitas, viabilitas, dan morfologi) spermatozoa (P?0,05), sehingga Biji Klabet (Trigonella foenum-graecum Linn.) memiliki potensi sebagai bahan alami kontrasepsi pria.
{"title":"Pemberian Ekstrak Etanol Biji Klabet (Trigonella foenum-graecum L.) Menurunkan Kualitas Spermatozoa Mencit (Mus musculus) Dewasa Muda","authors":"Putu Susilawati, B. K. Satriyasa, I. G. A. Widianti","doi":"10.24843/metamorfosa.2022.v09.i02.p11","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p11","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) dapat menurunkan kualitas spermatozoa mencit (Mus musculus) dewasa muda sebagai bahan kontrasepsi pria alami. Penelitian ini merupakan penelitian eksperimental dengan menggunakan rancangan penelitian post-test only control group design. Sampel penelitian ini terdiri dari 36 ekor mencit (Mus musculus) jantan galur Swiss Webster usia 2-3 bulan dengan berat badan 20-30 gram yang dibagi menjadi dua kelompok secara acak. Kelompok kontrol (P0) diberikan CMC Na 0,3% sebanyak 0,5 ml dan kelompok perlakuan (P1) diberikan ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) 0,4 mg/gram BB sebanyak 0,5 ml selama 36 hari per oral. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol Biji Klabet (Trigonella foenum-graecum Linn.) dapat menurunkan kualitas (konsentrasi, motilitas, viabilitas, dan morfologi) spermatozoa (P?0,05), sehingga Biji Klabet (Trigonella foenum-graecum Linn.) memiliki potensi sebagai bahan alami kontrasepsi pria. \u0000 ","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44842881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-19DOI: 10.24843/metamorfosa.2022.v09.i02.p12
Khotima Dwi Cahya, Retno Kawuri, I. M. S. Wijana
Athelia rolfsii can cause stem rot on soybean plants with symptoms of infection at the base of the stem bordering the soil surface that caused reduce soybean yields up to 75%. Disease control with chemical fertilizers have negative impact on the environment. Bacillus sp. as antagonistic agent thats known produced enzymes, antibiotics, and siderophore capable to suppressed pathogenic fungus growth. This study aims to determine the potential of isolates Bacillus sp. which was isolated from the groundnut rhizosphere to inhibit A. rolfsii in vitro and to determine concentration of culture Bacillus sp. to suppress the growth of A. rolfsii in green house. The research used a completely randomized design (RAL), where the treatment was derived from the concentration volume of culture Bacillus sp. which shows the best resistance zone. Parameter observed in this research were the inhibition zone, disease incidence, and disease severity. Data were analyzed used analysis of variance (ANOVA). The results obtained 6 isolates of Bacillus sp. Isolate Bacillus sp.1 as isolate with the best percentage of inhibition in suppressing the growth of A. rolfsii (79,44%) then tested on greenhouse. Culture Bacillus sp.1 (10 mL) with a density 1x108 cells/mL was able to suppress incidene of stem rot disease up to 53,38 ± 18,23% and the percentage of disease severity 67,78 ± 19,40% during 7 HST. Amistar top as positive control can supress percentage incidence and disease severity up to 86,68 ± 18,25%. Culture of Bacillus sp.1 can be used as an antagonizing agent in stem rot disease caused by A. rolfsii. Keyword: Antagonist, Athelia rolfsii, Bacillus sp., Soybean
{"title":"Potensi Bacillus sp. Sebagai Agen Antagonis Terhadap Athelia rolfsii Penyebab Busuk Pangkal Batang Kedelai (Glycine max L.)","authors":"Khotima Dwi Cahya, Retno Kawuri, I. M. S. Wijana","doi":"10.24843/metamorfosa.2022.v09.i02.p12","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p12","url":null,"abstract":"Athelia rolfsii can cause stem rot on soybean plants with symptoms of infection at the base of the stem bordering the soil surface that caused reduce soybean yields up to 75%. Disease control with chemical fertilizers have negative impact on the environment. Bacillus sp. as antagonistic agent thats known produced enzymes, antibiotics, and siderophore capable to suppressed pathogenic fungus growth. This study aims to determine the potential of isolates Bacillus sp. which was isolated from the groundnut rhizosphere to inhibit A. rolfsii in vitro and to determine concentration of culture Bacillus sp. to suppress the growth of A. rolfsii in green house. The research used a completely randomized design (RAL), where the treatment was derived from the concentration volume of culture Bacillus sp. which shows the best resistance zone. Parameter observed in this research were the inhibition zone, disease incidence, and disease severity. Data were analyzed used analysis of variance (ANOVA). The results obtained 6 isolates of Bacillus sp. Isolate Bacillus sp.1 as isolate with the best percentage of inhibition in suppressing the growth of A. rolfsii (79,44%) then tested on greenhouse. Culture Bacillus sp.1 (10 mL) with a density 1x108 cells/mL was able to suppress incidene of stem rot disease up to 53,38 ± 18,23% and the percentage of disease severity 67,78 ± 19,40% during 7 HST. Amistar top as positive control can supress percentage incidence and disease severity up to 86,68 ± 18,25%. Culture of Bacillus sp.1 can be used as an antagonizing agent in stem rot disease caused by A. rolfsii. \u0000Keyword: Antagonist, Athelia rolfsii, Bacillus sp., Soybean","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44438685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.24843/metamorfosa.2022.v09.i02.p09
Dewa Adi Putra, N. L. Watiniasih, A. Dewi
Plankton merupakan organisme yang hidupnya melayang-layang di perairan yang pergerakannya dipengaruhi oleh arus. Keberadaan plankton di suatu perairan dapat digunakan sebagai bioindikator karena memiliki tingkat kepekaan tinggi dan juga dipengaruhi oleh perubahan kondisi perairan. Perubahan-perubahan kondisi perairan dapat diakibatkan oleh adanya aktivitas masyarakat. Salah satu pantai yang banyak memiliki aktivitas masyakarat yaitu Pantai Samuh. Penelitian ini dilakukan pada bulan September-Oktober 2021 di Pantai Samuh. Terdapat 3 stasiun pengamatan yaitu stasiun I diperuntukkan sebagai pendaratan kapal, stasiun II umumnya digunakan sebagai tempat rekreasi dan aktifitas masyarakat berwisata, stasiun III diperuntukkan sebagai kawasan perhotelan. Metode yang digunakan mentode deskriptif kuantitatif. Penentuan stasiun dilakukan dengan metode purposive sampling. Berdasarkan hasil pengamatan fitoplankton yang didapatkan di Pantai Samuh sebanyak 4 kelas yakni Bacillariophceae (13 genus), Zygnemophyceae (1 genus), Cyanophyceae (2 genus) dan Dinophyceae (1 genus). Zooplankton yang di temukan di Pantai Samuh sebanyak 4 kelas yaitu Hexanauplia (1 genus), Branchiopoda (1 genus), Crustacea (1 genus) dan Monogonta (1 Genus). Nilai kelimpahan rata-rata fitoplankton yaitu sebesar 347 dan nilai kelimpahan rata-rata zooplankton yaitu sebesar 25, nilai indeks keanekaragaman rata-rata fitoplankton sebesar 1,56 dan nilai indeks keanekaragaman rata-rata zooplankton sebesar 0,79, nilai keseragaman rata-rata fitoplankton yaitu sebesar 0,72dan nilai keseragaman rata-rata zooplankton yaitu sebesar 0,96, nilai dominansi rata-rata fitoplankton yaitu sebesar 0,31 dan nilai dominansi rata-rata zooplankton yaitu sebesar 0,51.
{"title":"Struktur Komunitas Plankton di Pantai Samuh, Nusa Dua, Bali","authors":"Dewa Adi Putra, N. L. Watiniasih, A. Dewi","doi":"10.24843/metamorfosa.2022.v09.i02.p09","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p09","url":null,"abstract":"Plankton merupakan organisme yang hidupnya melayang-layang di perairan yang pergerakannya dipengaruhi oleh arus. Keberadaan plankton di suatu perairan dapat digunakan sebagai bioindikator karena memiliki tingkat kepekaan tinggi dan juga dipengaruhi oleh perubahan kondisi perairan. Perubahan-perubahan kondisi perairan dapat diakibatkan oleh adanya aktivitas masyarakat. Salah satu pantai yang banyak memiliki aktivitas masyakarat yaitu Pantai Samuh. Penelitian ini dilakukan pada bulan September-Oktober 2021 di Pantai Samuh. Terdapat 3 stasiun pengamatan yaitu stasiun I diperuntukkan sebagai pendaratan kapal, stasiun II umumnya digunakan sebagai tempat rekreasi dan aktifitas masyarakat berwisata, stasiun III diperuntukkan sebagai kawasan perhotelan. Metode yang digunakan mentode deskriptif kuantitatif. Penentuan stasiun dilakukan dengan metode purposive sampling. Berdasarkan hasil pengamatan fitoplankton yang didapatkan di Pantai Samuh sebanyak 4 kelas yakni Bacillariophceae (13 genus), Zygnemophyceae (1 genus), Cyanophyceae (2 genus) dan Dinophyceae (1 genus). Zooplankton yang di temukan di Pantai Samuh sebanyak 4 kelas yaitu Hexanauplia (1 genus), Branchiopoda (1 genus), Crustacea (1 genus) dan Monogonta (1 Genus). Nilai kelimpahan rata-rata fitoplankton yaitu sebesar 347 dan nilai kelimpahan rata-rata zooplankton yaitu sebesar 25, nilai indeks keanekaragaman rata-rata fitoplankton sebesar 1,56 dan nilai indeks keanekaragaman rata-rata zooplankton sebesar 0,79, nilai keseragaman rata-rata fitoplankton yaitu sebesar 0,72dan nilai keseragaman rata-rata zooplankton yaitu sebesar 0,96, nilai dominansi rata-rata fitoplankton yaitu sebesar 0,31 dan nilai dominansi rata-rata zooplankton yaitu sebesar 0,51.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47365779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-16DOI: 10.24843/metamorfosa.2022.v09.i02.p07
E. Hidayati, Muhammad Sarkono, Faturrahman Faturrahman
This research aimed to study the growth responses of maize and soybean to the indigenous bacterial consortium application isolated from Lombok dryland farm. The study was tested in the sterile soil and nonsterile soil of growing media and with a different water capacity of growing media. The soil was taken from North Lombok dryland farm. Sterile soil was prepared by sterilization using an autoclave. The bacterial consortium was prepared by mixing 15 inoculums of rhizosphere bacteria isolated from Lombok dryland farm. The consortium inoculation by soil inoculation technique. The water capacity of growing media was set to 25%, 50%, and 75%. This research was done in the greenhouse scale. Growth parameters that analyzed were leaf number, leaf wide, leaf length, plant length, fresh weight of upper biomass, and dry weight of upper biomass. The results showed that plant growth is better in sterile soil compared to nonsterile soil. Plant growth is better also in the sterile soil inoculated with the bacterial consortium compared to nonsterile soil inoculated with the bacterial consortium. Plant growth was better in 75% and 50% than 25% water holding capacity of growing Keyword: bacterial consortium, dryland farm, rhizosphere, North Lombok
{"title":"Growth Responses Of Maize And Soybean To Application Of Rhizosphere Bacterial Consortium From Dry Land North Lombok","authors":"E. Hidayati, Muhammad Sarkono, Faturrahman Faturrahman","doi":"10.24843/metamorfosa.2022.v09.i02.p07","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p07","url":null,"abstract":"This research aimed to study the growth responses of maize and soybean to the indigenous bacterial consortium application isolated from Lombok dryland farm. The study was tested in the sterile soil and nonsterile soil of growing media and with a different water capacity of growing media. The soil was taken from North Lombok dryland farm. Sterile soil was prepared by sterilization using an autoclave. The bacterial consortium was prepared by mixing 15 inoculums of rhizosphere bacteria isolated from Lombok dryland farm. The consortium inoculation by soil inoculation technique. The water capacity of growing media was set to 25%, 50%, and 75%. This research was done in the greenhouse scale. Growth parameters that analyzed were leaf number, leaf wide, leaf length, plant length, fresh weight of upper biomass, and dry weight of upper biomass. The results showed that plant growth is better in sterile soil compared to nonsterile soil. Plant growth is better also in the sterile soil inoculated with the bacterial consortium compared to nonsterile soil inoculated with the bacterial consortium. Plant growth was better in 75% and 50% than 25% water holding capacity of growing \u0000 \u0000Keyword: bacterial consortium, dryland farm, rhizosphere, North Lombok","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46456401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-14DOI: 10.24843/metamorfosa.2022.v09.i02.p05
Alia Ambara Putri Purbaya, L. Yuni, Fransiscus Xaverius Sudaryanto
The Southern Cassowary (Casuarius casuarius Linnaeus, 1758) is an endemic species whose presence has a major influence on forest ecology in Papua. As a frugivore, this bird plays a role in spreading the seeds of fruit trees which make up 90% of tree species in Papua's forests. However, the population of this bird in its natural habitat has decreased due to forest conversion as the population grows, and is exacerbated by hunting by the local community. Ex-situ conservation is carried out with the aim of breeding animals outside their natural habitat, so research on the daily activities of the Southern cassowary is required. This study was conducted from January 10 to February 21, 2022 on five individual Southern Cassowaries at the Bali Zoo. The method used was focal animal sampling with instantaneous recording for 30 minutes with one-minute intervals. The data obtained were tabulated and analyzed using Microsoft Excel, then described descriptively. The most common activity for the Southern Cassowary at Bali Zoo was resting (35.69%), followed by moving activities (19.69%), foraging (18.58%), preening (16.74%), alert (6.69%), vocalization (1.19%), defecation (0.48%), and urination (0.14%). This study shows that the Southern Cassowary can reflect its daily activities naturally while at the Bali Zoo conservation institution which indicates that animal welfare is fulfilled. Keyword : meloxicam, moringa oleifera, sperm quality, testosterone hormone
{"title":"Daily Activity of Southern Cassowary (Casuarius casuarius Linnaeus, 1758) in Bali Zoo, Gianyar","authors":"Alia Ambara Putri Purbaya, L. Yuni, Fransiscus Xaverius Sudaryanto","doi":"10.24843/metamorfosa.2022.v09.i02.p05","DOIUrl":"https://doi.org/10.24843/metamorfosa.2022.v09.i02.p05","url":null,"abstract":"The Southern Cassowary (Casuarius casuarius Linnaeus, 1758) is an endemic species whose presence has a major influence on forest ecology in Papua. As a frugivore, this bird plays a role in spreading the seeds of fruit trees which make up 90% of tree species in Papua's forests. However, the population of this bird in its natural habitat has decreased due to forest conversion as the population grows, and is exacerbated by hunting by the local community. Ex-situ conservation is carried out with the aim of breeding animals outside their natural habitat, so research on the daily activities of the Southern cassowary is required. This study was conducted from January 10 to February 21, 2022 on five individual Southern Cassowaries at the Bali Zoo. The method used was focal animal sampling with instantaneous recording for 30 minutes with one-minute intervals. The data obtained were tabulated and analyzed using Microsoft Excel, then described descriptively. The most common activity for the Southern Cassowary at Bali Zoo was resting (35.69%), followed by moving activities (19.69%), foraging (18.58%), preening (16.74%), alert (6.69%), vocalization (1.19%), defecation (0.48%), and urination (0.14%). This study shows that the Southern Cassowary can reflect its daily activities naturally while at the Bali Zoo conservation institution which indicates that animal welfare is fulfilled. \u0000Keyword : meloxicam, moringa oleifera, sperm quality, testosterone hormone \u0000 ","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49381256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：