Biomacromolecules最新文献_第7页

Bacteria-Induced Oligopeptide Assembly Enables Effective Bacterial Killing with Low Cytotoxicity and Hemolysis 细菌诱导的寡肽组装使低细胞毒性和溶血有效杀死细菌。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01737

Yi Cheng, He Zhao, Jiayi Sun, Shuaishuai Nie, Wen Li

Bacteria-induced on-membrane assembly of oligopeptides showed the potential for balancing their persistent conflict between antibacterial activity and biocompatibility. However, molecular determinants linking the peptide sequence, assembly behavior, antibacterial efficacy, and biocompatibility remain insufficiently understood. Here, we investigated a series of heptapeptides, (RX)₃R (X = A, I, F), and demonstrated the significant influence of hydrophobic residues on bacteria-induced assembly, antibacterial performance, cytotoxicity, and hemolytic activity. Negatively charged liposomes or bacterial membranes could induce the assembly of (RI)₃R and (RF)₃R into nanofibers, which in turn exhibited enhanced antibacterial efficacy against Gram-negative Escherichia coli with minimum inhibitory concentrations (MICs) of 60 μM but poor antibacterial activity on Gram-positive Staphylococcus aureus (MICs: 400–500 μM). Importantly, both peptides demonstrated excellent biocompatibility, with cytotoxicity thresholds of 600 μM (RI)₃R and 2000 μM (RF)₃R and hemolysis thresholds up to 2000 and 4000 μM, respectively. These findings provide valuable insights for the rational design of antibacterial oligopeptides.

细菌诱导的寡肽在膜上组装显示出平衡其抗菌活性和生物相容性之间持续冲突的潜力。然而，连接肽序列、组装行为、抗菌功效和生物相容性的分子决定因素仍未充分了解。在这里，我们研究了一系列七肽，（RX）3R (X = a， I， F)，并证明了疏水残基对细菌诱导的组装，抗菌性能，细胞毒性和溶血活性的显著影响。带负电荷的脂质体或细菌膜可诱导（RI）3R和（RF）3R组装成纳米纤维，纳米纤维对革兰氏阴性大肠杆菌的抑菌效果较好，最低抑菌浓度（mic）为60 μM，但对革兰氏阳性金黄色葡萄球菌（mic: 400-500 μM）的抑菌活性较差。重要的是，这两种肽均表现出良好的生物相容性，细胞毒性阈值分别为600 μM (RI)3R和2000 μM (RF)3R，溶血阈值分别为2000 μM和4000 μM。这些发现为抗菌寡肽的合理设计提供了有价值的见解。

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引用次数: 0

From Amorphous Bundles to Nanoporous Peptide Frameworks: Solvent-Driven Self-Assembly of Pro-Val-Pro-Val 从无定形束到纳米多孔肽框架：溶剂驱动的Pro-Val-Pro-Val自组装。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01800

Kacper Drużbicki , Piotr Paluch , Rafał Dolot , Irena Bąk-Sypień , Ewelina Kuc , Agata Jeziorna , Marek J. Potrzebowski

Peptides are ubiquitous and functionally diverse biomolecules with a strong propensity to form noncovalent nanostructures of unexpected complexity. Using nuclear magnetic resonance (NMR), X-ray diffraction, and computational modeling, we show that the amphiphilic tetrapeptide l-prolyl-l-valyl-l-prolyl-l-valine (PVPV) undergoes solvent-directed self-assembly into two molecular frameworks, each built upon a helical motif formed through charge-assisted hydrogen bonding. Water–methanol crystallization yields a solvent-stabilized porous tetragonal framework, whereas pure water produces either a low-hydration amorphous phase or a dense, disordered triclinic form. These behaviors challenge first-principles modeling and expose limitations of static semilocal density functional theory. Calculations indicate that the porous framework is energetically less favorable, highlighting the importance of water disorder and conformational flexibility in forming metastable structures. The low-hydration phase exhibits intrinsic conformational disorder, motivating an in-depth investigation via NMR crystallography. Advanced solid-state NMR on isotopically labeled samples provides assignments not achievable through conventional approaches, revealing subtle molecular distortions that modulate local ordering.

肽是一种普遍存在且功能多样的生物分子，具有形成非共价纳米结构的强烈倾向，具有意想不到的复杂性。利用核磁共振（NMR）、x射线衍射和计算模型，我们发现两亲性四肽l-丙氨酸-l-丙氨酸-l-丙氨酸-l-丙氨酸-l-丙氨酸-l-缬氨酸（PVPV）经历了溶剂定向自组装成两个分子框架，每个分子框架都建立在通过电荷辅助氢键形成的螺旋基序上。水-甲醇结晶产生溶剂稳定的多孔四边形框架，而纯水产生低水化无定形相或致密，无序的三斜形式。这些行为挑战了第一性原理建模，暴露了静态半局部密度泛函理论的局限性。计算表明，多孔框架在能量上不太有利，突出了水无序性和构象柔韧性在形成亚稳结构中的重要性。低水化相表现出固有的构象紊乱，激发了通过核磁共振晶体学的深入研究。在同位素标记的样品上，先进的固态核磁共振提供了传统方法无法实现的分配，揭示了调节局部排序的微妙分子扭曲。

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引用次数: 0

Unravelling the Complexity of LignosulfonatesFractionation and Physicochemical Profiling 揭示木质素磺酸盐的复杂性──分馏和物理化学分析。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02229

Veslemøy Margrethe Selvik , Finn Lillelund Aachmann , Carlos Salas-Bringas , Vebjørn Eikemo

Understanding the structural architecture of lignosulfonates is essential for optimizing their performance and enabling targeted modifications for sustainable applications. This study investigates six sodium lignosulfonate fractions (2,200–78,000 g/mol; dispersity 1.7–12.2) obtained via two-step ultrafiltration. Functional group analysis (sulfonation degree, phenolic hydroxyl, and carboxylic acid) using elemental analysis and NMR revealed minimal variation across fractions. Hydrophobic interaction chromatography and intrinsic viscosity measurements showed that increasing molecular weight correlates with reduced charge-to-size ratios and enhanced hydrophobicity. Rheological data showed that high-molecular-weight fractions exhibit greater conformational changes and compaction under high ionic strength. 2D NMR of purified fractions uncovered new structural features, including guaiacylethanol and fully characterized mono- and disulfonated bonding patterns. These findings advance the structural mapping of lignosulfonates and demonstrate that molecular weight is the dominant factor influencing the physical properties. The study highlights the value of combining fractionation, rheology, and NMR techniques to deepen our understanding of lignosulfonate structure and guide future lignin-based materials applications.

了解木质素磺酸盐的结构结构对于优化其性能和实现可持续应用的针对性修改至关重要。研究了两步超滤得到的6个木质素磺酸钠馏分（2200 ~ 78000 g/mol，分散度1.7 ~ 12.2）。使用元素分析和核磁共振的官能团分析（磺化程度、酚羟基和羧酸）显示各馏分之间的变化最小。疏水相互作用色谱和特性粘度测量表明，分子量的增加与电荷尺寸比的降低和疏水性的增强有关。流变学数据表明，高分子量组分在高离子强度下表现出更大的构象变化和压实。纯化馏分的二维核磁共振揭示了新的结构特征，包括愈创木基乙醇和完全表征的单磺酸和二磺酸键模式。这些发现促进了木质素磺酸盐的结构作图，并证明分子量是影响其物理性质的主要因素。该研究强调了将分选、流变学和核磁共振技术相结合的价值，以加深我们对木质素磺酸盐结构的理解，并指导未来木质素基材料的应用。

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引用次数: 0

GSH-Responsive Prodrug Polymersomes for Copper-Free Cuproptosis and Synergistic Photothermal Cancer Therapy gsh反应性前药聚合体用于无铜铜增生和协同光热癌症治疗。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01893

Fangrong Tan, Yuandong Zeng, Donghua Dong, Yan Deng, Zhe Tang, LinGe Wang, Qianqian Yu

Cuproptosis offers a promising alternative to apoptosis-based cancer therapies but faces challenges, such as poor tumor drug accumulation, rapid clearance of copper ionophores, and high intracellular glutathione (GSH). Herein, we developed a GSH-responsive prodrug polymersome (PED@ICG) for copper-independent cuproptosis and synergistic photothermal therapy. Diethyldithiocarbamate (DTC) was covalently attached via disulfide bonds, ensuring a GSH-triggered release and intracellular GSH depletion. The amphiphilic polyprodrug formed stable polymersomes, and ICG coloading endowed imaging ability and NIR-activated photothermal heating. PED@ICG exhibited efficient cellular uptake through dynamin-mediated endocytosis, achieved deep tumor penetration, and selectively released DTC within the reductive tumor microenvironment. In vitro and in vivo studies demonstrated potent anticancer efficacy without the need for exogenous copper, while NIR irradiation further enhanced therapeutic outcomes through localized hyperthermia. This work offers a promising strategy for precise cuproptosis modulation and photothermal synergism, providing a clinically translatable nanoplatform for copper-independent tumor therapy.

铜细胞凋亡是基于细胞凋亡的癌症治疗的一个有希望的替代方案，但面临着挑战，如肿瘤药物积累差，铜离子载体的快速清除和高细胞内谷胱甘肽（GSH）。在此，我们开发了一种gsh反应性前药聚合体（PED@ICG），用于不依赖铜的铜还原和协同光热治疗。二乙基二硫代氨基甲酸酯（DTC）通过二硫键共价连接，确保GSH触发的释放和细胞内GSH消耗。两亲性聚前药形成稳定的聚合体，ICG负载赋予成像能力和nir激活的光热加热。PED@ICG通过动力蛋白介导的内吞作用表现出有效的细胞摄取，实现了肿瘤深度渗透，并在还原性肿瘤微环境中选择性释放DTC。体外和体内研究表明，不需要外源性铜就能有效地抗癌，而近红外照射通过局部热疗进一步提高了治疗效果。这项工作提供了一种有前景的策略，用于精确的铜生长调节和光热协同作用，为不依赖铜的肿瘤治疗提供了临床可翻译的纳米平台。

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引用次数: 0

Redox-Responsive Polyurethane Nanonetworks with Tunable Cross-linking: A Robust Platform for Cocktail Chemotherapy 具有可调交联的氧化还原反应聚氨酯纳米网络：鸡尾酒化疗的强大平台。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01180

Soumya Kolay , Madhuchhanda Das , Sananda Dey , Tanushree Mondal , Arun Mondal , Subhadeep Kar , Arunima Sengupta , Subhash Haldar , Biplab Giri , Mijanur Rahaman Molla

We formulated a nanonetwork of an amphiphilic polyurethane integrated with a tertiary amine group on the backbone and a pyridyl disulfide group as a pendant. The disulfide linkages within the nanonetwork undergo reductive cleavage in the intracellular redox environment of cancer cells, enabling sustained and site-specific drug release. The positive surface-charge generation under tumor extracellular matrix-like mildly acidic conditions promotes selective uptake of the nanonetwork by cancer cells while minimizing interaction with normal cells, as shown by flow cytometry analysis. Biological evaluation confirmed selective internalization and potent cytotoxicity in HeLa and MDA-MB-231 cells following treatment with NN@DOX, while normal cells were effectively shielded from off-target toxicity. Furthermore, the dual-drug-loaded nanonetwork (NN@DOX-CPT) exhibited superior therapeutic outcomes, as demonstrated by a significantly lower IC₅₀ (3.98 μg/mL) value compared to single-drug formulations, NN@DOX (IC₅₀ = 9.89 μg/mL), confirming a combined effect. Overall, this polymeric nanonetwork could be a promising system for combination drug-based targeted chemotherapy.

我们配制了一种两亲性聚氨酯的纳米网络，在主链上集成了叔胺基团和吡啶二硫化基作为垂坠。纳米网络中的二硫键在癌细胞的细胞内氧化还原环境中进行还原性裂解，从而实现持续和位点特异性的药物释放。流式细胞术分析显示，在肿瘤细胞外基质样的温和酸性条件下，正表面电荷的产生促进了癌细胞对纳米网络的选择性摄取，同时最大限度地减少了与正常细胞的相互作用。生物学评价证实了NN@DOX治疗后HeLa和MDA-MB-231细胞的选择性内化和强大的细胞毒性，而正常细胞有效地屏蔽了脱靶毒性。此外，双药负载纳米网络（NN@DOX-CPT）表现出更好的治疗效果，其IC50值（3.98 μg/mL）显著低于单药配方NN@DOX (IC50 = 9.89 μg/mL)，证实了联合效应。总的来说，这种聚合物纳米网络可能是一种很有前途的基于联合药物的靶向化疗系统。

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引用次数: 0

Highly Tough, Barrier, and Biodegradable Copolyesters Synthesized from Furandicarboxylic Acid 由呋喃二羧酸合成的高韧性、阻隔性和可生物降解共聚聚酯。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02108

Jiangwei Zhao , Yunxiao Dong , Jin Zhu , Jinggang Wang

Biobased polyesters based on 2,5-furandicarboxylic acid show great potential for replacing conventional plastics, but their practical applications are still limited by the inherent brittleness of the materials. A series of high molecular-weight poly(ethylene-diethylene glycol 2,5-furandicarboxylate) (PEDF) copolyesters were synthesized by introducing diethylene glycol, which contained a flexible oxygen ether bond. The thermal stability, mechanical properties, and optical transparency of the PEDF copolyesters improved significantly with the increasing diethylene glycol content. The elongation at break of PED₄₀F can reach 43% which is up to 10.7 folds compared with PEF and maintains a high tensile modulus of 2440 MPa and a tensile strength of 74 MPa, as well as a mass loss of 45.79% after 49 days under CALB enzymatic degradation conditions. In addition, PEDF copolyester has excellent gas barrier properties, with carbon dioxide and oxygen barrier improvement factors (BIFp) 13.0 and 7.3 times higher than those of PET.

基于2,5-呋喃二羧酸的生物基聚酯显示出取代传统塑料的巨大潜力，但其实际应用仍然受到材料固有脆性的限制。通过引入含柔性氧醚键的二甘醇，合成了一系列高分子量聚乙二醇-二甘醇2,5-呋喃二羧酸酯（PEDF）共聚酯。随着二甘醇含量的增加，PEDF共聚酯的热稳定性、力学性能和光学透明度显著提高。在CALB酶解条件下，pe40f的断裂伸长率可达43%，是PEF的10.7倍，并在49天后保持2440 MPa的高抗拉模量和74 MPa的抗拉强度，质量损失率为45.79%。此外，PEDF共聚酯具有优异的气体阻隔性能，其二氧化碳和氧气阻隔改善因子（BIFp）分别是PET的13.0和7.3倍。

引用次数: 0

Design and Synthesis of an Aggregation-Induced Emission-Active Quad-Functional “Curcumin-Spiced Marvel” for Engineering of Sialic Acid-Targeted Nanoplatform for Cancer Therapy 聚合诱导发射-活性四功能“姜黄素香料奇迹”的设计与合成，用于唾液酸靶向癌症治疗的纳米平台工程。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02001

Twara Kikani , Krutika Patel , Aneri Joshi , Devanshi Gajjar , Sriram Seshadri , Sonal Thakore

Aggregation-Induced Emission (AIE) is a feature of fluorophores that overcomes the demerits of Aggregation-Caused Quenching (ACQ), which limits the utility of traditional dyes in cancer bioimaging. Tetraphenylethene (TPE) is the prototypical AIEgen among the very few compounds with this property. However, it lacks inherent targeting specificity or therapeutic functions. In an attempt to design an AIE-active, target-specific therapeutic agent, we herein report a quad-functional AIEgen derived from the golden spice – curcumin. The “Golden Spiced AIEgen” was engineered by introducing a phenylboronic acid group into curcumin, imparting pH-responsive and sialic acid-targeting properties. Further integration into self-assembling amphiphilic micelles co-loaded with chemotherapeutics enabled effective loading with extended release. The integrated nanosystem was fully biocompatible with noncancerous cells while effectively killing HepG2 liver cancer cells through sialic acid-mediated AIEgen uptake. It acted synergistically with chemotherapeutic drugs for improved treatment outcomes, and its efficacy was corroborated in hepatocarcinoma in vivo.

聚集诱导发射（AIE）是荧光团的一个特征，它克服了聚集引起猝灭（ACQ）的缺点，这限制了传统染料在癌症生物成像中的应用。四苯基乙烯（TPE）是具有这种性质的极少数化合物中的典型化合物。然而，它缺乏固有的靶向特异性或治疗功能。为了设计一种具有aie活性的靶向治疗药物，我们在此报道了一种从黄金香料-姜黄素中提取的四功能AIEgen。“黄金香料AIEgen”是通过在姜黄素中引入苯硼酸基团来设计的，赋予ph响应和唾液酸靶向特性。进一步整合到自组装的两亲性胶束中，与化疗药物共载，使其有效加载并延长释放。该集成纳米系统与非癌细胞具有完全的生物相容性，同时通过唾液酸介导的AIEgen摄取有效杀死HepG2肝癌细胞。它与化疗药物协同作用，改善治疗效果，其有效性在体内肝癌中得到证实。

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引用次数: 0

Cholesterol-Rich Membranes Wetting by Polypeptide Coacervate: Pathway and Mechanism via the Liquid-Disordered Phase 多肽凝聚体润湿富含胆固醇的膜：液体无序相的途径和机制。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02341

Yiwei Wang, Rongrong Zou, Minghao Wang, Yeqiang Zhou, Yang Liu, Mingming Ding

Polypeptide coacervates exhibit remarkable cell membrane permeability for drug delivery, but the precise internalization mechanism is unclear. Here, taking histidine-rich beak derivative protein (HBpep-SR) coacervate as a model, we investigate the interactions of coacervates with ternary lipid raft membranes and mammalian plasma membranes using the Martini 3.0 force field. We show that coacervates preferentially wet the liquid disordered (Ld) phase in a cholesterol-dependent manner with an encapsulation efficiency in the Ld phase of approximately 60%. In rigid, cholesterol-depleted ternary membranes, coacervates fail to induce membrane bending and disperse over time. Conversely, the flexibility of unsaturated lipids in the Ld phase of lipid rafts promotes coacervate wrapping, a process mediated by aromatic rings in polypeptides. Similar trends are observed in plasma membrane systems and other polypeptide coacervate systems. Our findings reveal a universal pathway for coacervate uptake via Ld regions, offering crucial insights for designing coacervates with enhanced cellular internalization.

多肽凝聚体具有明显的细胞膜渗透性，但其内化机制尚不清楚。本研究以富组氨酸喙衍生蛋白（hbpeg - sr）凝聚体为模型，利用Martini 3.0力场研究凝聚体与三元脂筏膜和哺乳动物质膜的相互作用。我们发现凝聚体以胆固醇依赖的方式优先湿润液态无序（Ld）相，Ld相的包封效率约为60%。在刚性，胆固醇耗尽三元膜，凝聚体不能诱导膜弯曲和分散随着时间的推移。相反，在脂筏的Ld期，不饱和脂质的柔韧性促进凝聚包膜，这是一个由多肽中的芳香环介导的过程。在质膜系统和其他多肽凝聚系统中也观察到类似的趋势。我们的研究结果揭示了凝聚体通过Ld区域摄取的普遍途径，为设计增强细胞内化的凝聚体提供了重要的见解。

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引用次数: 0

Distinct Cation Roles and Shared Anion Mechanism in Hydroxide-Based Cellulose Solvents. 羟基纤维素溶剂中不同阳离子的作用和共用阴离子机制。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02076

Lingfeng Zhou, Wenze Cao, Jinghua Wu, Yiwei Li, Jierui Ye, Jingyu Li, Haisong Qi, Jie Cai, Ang Lu, Pan Chen

Cellulose dissolution remains a fundamental challenge due to its recalcitrant crystalline structure, governed by interchain hydrogen bonds and dispersion interactions. Hydroxide-based systems are industrially relevant but require energy-intensive subzero temperatures. This study employs molecular dynamics simulations to elucidate the molecular mechanisms underlying the dissolution performance of benzyltrimethylammonium hydroxide (BzMe₃NOH) and NaOH. Na⁺ binds to cellulose primarily through electrostatic interactions, whereas the amphiphilic benzyltrimethylammonium cation (BzMe₃N⁺) engages predominantly via vdW interactions, accumulating along the hydrophobic backbone. Both systems exhibit anion-cellulose interactions with hydroxide ions, forming bifurcated hydrogen bonds that facilitate transient deprotonation of hydroxyl groups. A key thermodynamic advantage of BzMe₃N⁺ is that each cation displaces more water molecules away from cellulose's solvation shell than Na⁺ does, reducing the entropic penalty of dissolution. This work establishes that effective dissolution in hydroxide systems requires a synergistic combination of anion-driven hydrogen-bonding disruption and cation-driven dispersion compensation.

纤维素溶解仍然是一个基本的挑战，由于其顽固性的晶体结构，由链间氢键和分散相互作用。基于氢氧化物的系统在工业上是相关的，但需要在零度以下的温度下消耗大量能源。本研究采用分子动力学模拟来阐明苯三甲基氢氧化铵（BzMe3NOH）和氢氧化钠溶解性能的分子机制。Na+主要通过静电相互作用与纤维素结合，而两亲性的苄基三甲基铵阳离子（BzMe3N+）主要通过vdW相互作用与纤维素结合，沿着疏水主链积累。这两种体系都表现出阴离子-纤维素与氢氧根离子的相互作用，形成分叉的氢键，促进羟基的瞬时去质子化。BzMe3N+的一个关键热力学优势是，每个阳离子都比Na+从纤维素的溶剂化壳中置换出更多的水分子，从而减少了溶解的熵损失。这项工作表明，氢氧化物体系中的有效溶解需要阴离子驱动的氢键破坏和阳离子驱动的分散补偿的协同组合。

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引用次数: 0

Anisotropic Janus Hydrogel Patch for Abdominal Wall Regeneration and Postoperative Antiadhesion 各向异性Janus水凝胶贴片用于腹壁再生和术后抗粘连。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02331

Penghui Wang , Jialing Li , Yingying Yang , Wenjie Zhang , Ji Zhu , Bo Chi

The reconstruction of abdominal wall defects and prevention of postoperative visceral adhesions remain critical clinical challenges in managing abdominal trauma. Here, a highly bioactive macroporous hyaluronic acid hydrogel was firmly integrated onto the upper layer of the poly(vinyl alcohol) hydrogel through dynamic borate ester bonds to construct the biomimetic repair patch (PHA). The anisotropic backbone prepared based on a top-down solvent exchange strategy achieved strong mechanical strength (2.69 MPa), excellent elastic deformation (369.73%), and biomimetic structure of asymmetric porous surfaces with upper (79.62 μm) and lower (0.63 μm) pore sizes. The in vivo abdominal wall defect repair model demonstrated that the asymmetric porous structure of the PHA patch simultaneously enables in situ regeneration of abdominal wall tissues and suppression of visceral adhesions. Therefore, the design of the PHA patch opens a new avenue for developing an integrated biomimetic abdominal wall material, holding significant practical implications for enhancing the clinical treatment efficacy of abdominal wall defects.

腹壁缺损的重建和术后内脏粘连的预防仍然是处理腹部创伤的关键临床挑战。在这里，高生物活性的大孔透明质酸水凝胶通过动态硼酸酯键牢固地集成到聚乙烯醇水凝胶的上层，构建仿生修复贴片（PHA）。基于自顶向下溶剂交换策略制备的各向异性骨架具有较强的机械强度（2.69 MPa）、优异的弹性变形（369.73%）和上孔径（79.62 μm）和下孔径（0.63 μm）的非对称多孔表面仿生结构。体内腹壁缺损修复模型表明，PHA贴片的不对称多孔结构可以同时实现腹壁组织的原位再生和内脏粘连的抑制。因此，PHA贴片的设计为开发一种集成的仿生腹壁材料开辟了新的途径，对提高腹壁缺陷的临床治疗效果具有重要的现实意义。

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引用次数: 0