Biomacromolecules最新文献_第10页

Redox-Responsive Self-Assembled Amphiphilic Nanosheets from Polyglycerol Sulfate–Lipoic Acid Copolymers for Targeted Cancer Drug Delivery 由聚甘油硫酸盐-硫辛酸共聚物组成的氧化还原反应自组装两亲性纳米片用于靶向癌症药物递送。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01204

Taylor M. Page , Kai Ludwig , Muhammad Shayan Haider , Elisa Quaas , Alexandros Mavroskoufis , Peng Tang , Rui Chen , Jun Feng, Raju Bej , Katharina Achazi , Rainer Haag , Ievgen S. Donskyi

Targeted drug delivery systems that are stimuli-responsive offer great potential for enhancing the therapeutic activity of drugs, decreasing off-target effects, and improving bioavailability. This proof-of-concept study introduces an amphiphilic drug delivery system (DDS) capable of loading hydrophobic cargo. Elevated glutathione (GSH) levels, characteristic of certain types of cancer cells’ microenvironment, degrade the nanostructures and release the cargo. Linear polyglycerol sulfate (LPGS), known for its excellent biocompatibility, is combined with lipoic acid (LA). LA facilitates the formation of cross-linked nanosheet amphiphiles sensitive to reductive conditions. Morphological changes are observed by scanning electron microscopy (SEM), cryogenic transmission electron microscopy (Cryo-TEM), and cryogenic electron tomography (Cryo-ET) upon UV irradiation (hν), creating a stable aggregate for loading hydrophobic cargo and assembling into sheets at elevated concentrations. The resulting material displays controlled release of model dyes under increased levels of GSH, tunable by the polymer size and LPGS:LA acid ratios. This behavior enhances targeted therapy and reduced off-target effects. Further loading with paclitaxel and subsequent release, together with in vitro assays, demonstrates the system’s compatibility with an anticancer drug.

刺激反应性的靶向药物传递系统在增强药物的治疗活性、减少脱靶效应和提高生物利用度方面提供了巨大的潜力。这项概念验证研究介绍了一种能够装载疏水货物的两亲性药物输送系统（DDS）。谷胱甘肽（GSH）水平升高是某些类型癌细胞微环境的特征，它会降解纳米结构并释放货物。线性聚甘油硫酸盐（LPGS）以其优异的生物相容性而闻名，与硫辛酸（LA）结合。LA促进了对还原条件敏感的交联两亲纳米片的形成。通过扫描电子显微镜（SEM）、低温透射电子显微镜（Cryo-TEM）和低温电子断层扫描（Cryo-ET）观察到紫外照射（hν）后的形态变化，形成稳定的聚集体，用于装载疏水货物并在高浓度下组装成薄片。所得到的材料在GSH水平增加的情况下显示出模型染料的可控释放，可通过聚合物尺寸和LPGS:LA酸比进行调节。这种行为增强了靶向治疗，减少了脱靶效应。进一步装载紫杉醇和随后的释放，以及体外试验，证明了该系统与抗癌药物的相容性。

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引用次数: 0

Fish-Based Biopolymer Complex Coacervate Coating for Improved Paper Oxygen and Water Barrier 鱼基生物聚合物复合凝聚涂层改善纸张的氧和水阻隔性。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02091

Sarah G. Fisher, Zachary Buck, Margaret J. Karim, Jaime C. Grunlan

Food packaging is critical to prevent food waste, but most of the packaging used today is not sustainable. Paper-based packaging materials offer a renewable option, but exhibit poor resistance to common permeants such as oxygen, grease, and water vapor. In this work, a complex coacervate coating is prepared from two waste biopolymers, gelatin and DNA, and applied to kraft paper to substantially improve its barrier properties. Thermally curing the coating after deposition decreases the water vapor transmission rate and oxygen transmission rate by 83 and 99%, respectively, relative to uncoated paper. This work represents one of the best fully biobased barrier coatings reported for paper and is a promising option for sustainable food packaging.

食品包装对防止食物浪费至关重要，但今天使用的大多数包装都是不可持续的。纸基包装材料提供了一种可再生的选择，但对氧气、油脂和水蒸气等常见渗透物的抵抗力较差。本文以明胶和DNA两种废生物聚合物为原料，制备了一种复合凝聚膜，并将其应用于牛皮纸上，大大提高了牛皮纸的阻隔性能。与未涂布纸相比，涂布后的涂层经热固化后，其水蒸气透过率和氧气透过率分别降低了83%和99%。这项工作代表了最好的全生物基屏障涂料之一，是可持续食品包装的一个有前途的选择。

引用次数: 0

Protein Self-Assembly States Modulate Lithium Carbonate Biomineralization: From Ion Chelation to Nucleation Sites 蛋白质自组装状态调节碳酸锂生物矿化：从离子螯合到成核位点。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01601

Zhichun Lin, Yizhen Yan , Archie Hunter, Huaiyu Yang

Understanding protein–salt interactions is important for controlling crystallization, including biomineralization, biopharmaceutical purification, biocatalytic enzymes, and environmental biointerfaces. This study for the first time investigated interactions between three proteins (lysozyme, red fluorescence protein, and bovine hemoglobin) and Li₂CO₃ (formation from the reaction of LiCl with Na₂CO₃) under different protein and salt concentrations. For the crystallization of Li₂CO₃, at low supersaturation (S), the proteins inhibited Li₂CO₃ nucleation by 20–40% through chelation. At high S, the proteins accelerated nucleation by 10–40%. The dual effects of the protein on Li₂CO₃ biomineralization have been discussed. With the increase S of Li₂CO₃, the dispersion state of proteins in solution undergoes a transition from dimers to oligomers and finally to aggregates. In all ranges of S, the protein reduced the agglomeration of Li₂CO₃ crystals. In lysozyme crystallization, increasing the Li₂CO₃ concentration yielded a larger number of smaller crystals. At equal concentration of lysozyme, twice more of LiCl and Na₂CO₃ in the solution led to more than 5 times the crystal number and 5 times smaller average crystal size. The interactions among protein molecules, salt ions in solution, and Li₂CO₃ crystals have been discussed. Dynamic light scattering measurements and the fluorescence microscopy image suggest that the dual effect of proteins on Li₂CO₃ crystallization at different supersaturation levels is associated with protein molecular aggregation under varying salt concentrations, resulting in both thermodynamic and kinetic influences on the crystallization process.

了解蛋白质-盐相互作用对于控制结晶非常重要，包括生物矿化、生物制药纯化、生物催化酶和环境生物界面。本研究首次研究了三种蛋白（溶菌酶、红色荧光蛋白和牛血红蛋白）在不同蛋白和盐浓度下与Li2CO3（由LiCl与Na2CO3反应生成）的相互作用。对于Li2CO3的结晶，在低过饱和度(S)下，蛋白质通过螯合作用抑制了Li2CO3的成核20-40%。在高S下，蛋白质的成核速度加快了10-40%。讨论了该蛋白对Li2CO3生物矿化的双重作用。随着Li2CO3浓度的增加，蛋白质在溶液中的分散状态经历了从二聚体到低聚体再到聚集体的转变。在S的所有范围内，该蛋白都减少了Li2CO3晶体的团聚。在溶菌酶结晶过程中，增加Li2CO3浓度会产生更多的小晶体。在溶菌酶浓度相同的情况下，溶液中LiCl和Na2CO3含量增加2倍，晶体数增加5倍以上，平均晶体尺寸减小5倍。讨论了蛋白质分子、溶液中的盐离子和Li2CO3晶体之间的相互作用。动态光散射测量和荧光显微镜图像表明，不同过饱和水平下蛋白质对Li2CO3结晶的双重影响与不同盐浓度下蛋白质分子聚集有关，从而对结晶过程产生热力学和动力学影响。

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引用次数: 0

Molecular Bottlebrush-Stabilized Polymeric Microspheres by Photoinitiated RAFT Dispersion Polymerization for Bead-Based Immunoassays 光引发RAFT分散聚合的瓶刷稳定聚合物微球用于珠基免疫分析。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02107

Wenyu Zhu , Yiying Ye , Li Zhang , Guangyao Zhao , Jianbo Tan

Polymeric microspheres are indispensable in catalysis, coatings, and biomedical analysis, yet conventional dispersion polymerization requires high stabilizer loadings and often yields surfaces with limited functional accessibility. Here, we report a molecular bottlebrush strategy that overcomes these limitations by employing reversible addition–fragmentation chain transfer (RAFT)-derived macromonomers, followed by ring-opening metathesis polymerization (ROMP) to generate bottlebrush stabilizers for photoinitiated RAFT dispersion polymerization. These bottlebrushes afford robust steric stabilization at significantly reduced loadings and enrich microsphere surfaces with RAFT end groups, enabling facile postpolymerization modification. The resulting PMMA microspheres exhibit exceptional monodispersity, tunable diameters, and versatile surface functionality. Subsequent photoiniferter polymerization yields carboxyl-functionalized microspheres with grafted poly(acrylic acid) chains, providing a high density of accessible carboxyl groups for biomacromolecule conjugation. Optimization of the chain length reveals a critical balance between loading capacity and accessibility, leading to markedly improved performance in bead-based immunoassays. This bottlebrush-mediated approach thus establishes a general and scalable platform for producing size-controlled, surface-functional polymeric microspheres with enhanced bioanalytical utility.

聚合物微球在催化、涂料和生物医学分析中是不可或缺的，然而传统的分散聚合需要高稳定剂负载，并且通常产生的表面功能有限。在这里，我们报道了一种分子瓶刷策略，通过采用可逆加成-破碎链转移（RAFT）衍生的大单体，然后通过开环分解聚合（ROMP）产生用于光引发RAFT分散聚合的瓶刷稳定剂，克服了这些限制。这些瓶刷在显著降低负载的情况下提供了强大的空间稳定性，并在微球表面丰富了RAFT端基，使聚合后修饰变得容易。由此产生的PMMA微球具有优异的单分散性、可调直径和多功能表面功能。随后的光干扰聚合产生带有接枝聚丙烯酸链的羧基功能化微球，为生物大分子偶联提供了高密度的可接近羧基。优化链长揭示了负载能力和可及性之间的关键平衡，从而显著提高了基于珠的免疫测定的性能。因此，这种瓶刷介导的方法建立了一个通用的、可扩展的平台，用于生产具有增强生物分析效用的尺寸控制、表面功能的聚合物微球。

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引用次数: 0

Angle- and Lateral Drag-Dependent Pull-Off Behavior of a Single Gecko Spatula: Insights from a Concurrent Molecular-Continuum Model 单个壁虎抹刀的角度和横向阻力依赖的拔离行为：来自并发分子连续体模型的见解。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01991

Saeed Norouzi , Tobias Materzok , Stanislav Gorb , Florian Müller-Plathe

Geckos adhere reliably to vertical and inverted surfaces, making them an appealing model for biomimetic adhesives. Yet a complete understanding of how this adhesion works remains elusive, primarily because the underlying mechanisms span multiple length scales. We used a hybrid particle-continuum model to simulate a gecko seta during pull-off tests. We examined how dragging the seta laterally on the substrate, varying drag distance and direction (distal or proximal), and the seta stalk angle influence the pull-off force. Across all conditions, increasing the drag distance raised the pull-off force. This resulted from a reduced peel-off angle before detachment, not from a larger contact area. Higher stalk angles (62° and 72°) also increased adhesion via a lower peel-off angle. Furthermore, distal dragging yielded up to 50% greater pull-off forces than proximal dragging, revealing anisotropic, directionally controllable adhesion. These results clarify how adhesion is tuned and suggest principles for bioinspired, direction-switchable adhesives.

壁虎可靠地粘附在垂直和倒置的表面，使它们成为仿生粘合剂的一个吸引人的模型。然而，完全理解这种粘附是如何工作的仍然是难以捉摸的，主要是因为潜在的机制跨越多个长度尺度。我们使用混合粒子-连续体模型来模拟下入测试中的壁虎集。我们研究了在基底上横向拖拽植骨、改变拖拽距离和方向（远端或近端）以及植骨柄角度对拔离力的影响。在所有情况下，增加阻力距离可以提高拉离力。这是由于剥离前的剥离角度减小，而不是由于更大的接触面积。较高的柄角（62°和72°）也通过较低的剥离角增加附着力。此外，远端拉力比近端拉力高出50%，显示出各向异性、方向可控的粘附性。这些结果阐明了粘合是如何调节的，并提出了生物启发的、方向可切换的粘合剂的原则。

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引用次数: 0

Silk Fibroin Hydrospongel with Interconnected Porosity and Robust Adhesion for Rapid Hemostasis and Scarless Repair 丝素蛋白水海绵具有相互连接的孔隙和强大的粘连性，用于快速止血和无疤痕修复。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02040

Yajing Liu , Bo Zou , Zhenhao Teng , Litao Wang , Ning Zhang , Na Zhao , Xiaofeng Wang , Qian Li , Xiaomeng Li

Rapid hemorrhage control and effective tissue regeneration are essential for improving outcomes in trauma and surgical wound management. Adhesive hydrogels adapt to irregular wounds but lack sufficient interconnected macroporosity for effective cell infiltration and tissue remodeling. Herein, a macroporous silk fibroin methacryloyl (SilMA) hydrospongel was developed via a simple foaming strategy. The foamed hydrospongel exhibits gel-like behavior before cross-linking and can be further stabilized through in situ photo-cross-linking. The SilMA hydrospongel achieves rapid hemostasis by absorbing blood and enhancing platelet aggregation and clot formation. Beyond hemostatic performance, its interconnected porous structure facilitates cellular infiltration and supports tissue integration, while its conformability and adhesion ensure seamless conformity to irregular wounds. Furthermore, the hydrospongel reduces fibrosis and scar formation in cutaneous and cardiac wounds by modulating the immune microenvironment. Collectively, this study demonstrates the translational potential of the hydrospongel as a multifunctional system for hemostasis, tissue regeneration, and drug delivery.

快速出血控制和有效的组织再生对于改善创伤和外科伤口处理的结果至关重要。黏附水凝胶适应于不规则的伤口，但缺乏足够的相互连接的大孔隙来有效的细胞浸润和组织重塑。本文通过简单的发泡策略制备了一种大孔丝素甲基丙烯酰（SilMA）水海绵。泡沫水凝胶在交联前表现出凝胶样行为，并且可以通过原位光交联进一步稳定。SilMA水海绵通过吸收血液和促进血小板聚集和凝块形成来实现快速止血。除了止血功能外，其相互连接的多孔结构促进细胞浸润并支持组织整合，而其顺应性和粘附性确保了不规则伤口的无缝整合。此外，水海绵通过调节免疫微环境减少皮肤和心脏伤口的纤维化和瘢痕形成。总的来说，这项研究证明了水凝胶作为止血、组织再生和药物输送的多功能系统的转化潜力。

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引用次数: 0

Ligand-Mediated Protein Corona on MIL-101(Fe) Governs Cytotoxicity via a Structure-Protein-Cell Cascade MIL-101（Fe）上配体介导的蛋白冕通过结构-蛋白-细胞级联调控细胞毒性。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c02147

Yi-Bo Yuan, Miao-Miao Yin, Zhi-Yu Zuo, Na Zhang, He-Chang Huang, Meng-Kang Jiang, Xin Ding, Yan-Jun Hu

Metal–organic frameworks’ (MOFs) biomedical performance is largely determined by surface ligands and the “protein corona” that forms in biological environments. Corona composition critically influences MOF behavior, yet how ligands direct corona formation and subsequent structure-protein-cell cascades remains poorly understood. Here, using MIL-101(Fe) as a model, three functionalized derivatives (H-, NH₂-, NO₂-MIL-101(Fe)) were constructed via electron-donating and electron-withdrawing groups. Ligand electronic effects critically modulate binding to serum proteins (HSA and transferrin), yielding distinct affinities (H ≥ NH₂ > NO₂) and interaction mechanisms (hydrogen bonding vs electrostatic). Cellular studies revealed that HSA coronas enhance the biocompatibility of MOFs in normal hepatocytes, while TRF coronas promote uptake, reactive oxygen species generation, and mitochondrial damage in 4T1 cancer cells, thereby amplifying cytotoxicity. This work systematically elucidates how ligand functionalization orchestrates protein corona structure and the subsequent protein-cell cascade, providing a mechanistic basis and design strategy for precise biomedical applications of MOFs.

金属有机框架（mof）的生物医学性能在很大程度上取决于表面配体和在生物环境中形成的“蛋白质冠”。电晕组成严重影响MOF行为，但配体如何直接电晕形成和随后的结构-蛋白质-细胞级联仍然知之甚少。本文以MIL-101（Fe）为模型，通过给电子和吸电子基团构建了3个功能化衍生物（H-、NH2-、NO2-MIL-101(Fe)）。配体电子效应严重调节与血清蛋白（HSA和转铁蛋白）的结合，产生不同的亲和力（H ≥ NH2 > NO2）和相互作用机制（氢键vs静电）。细胞研究表明，HSA冠状体增强了正常肝细胞中mof的生物相容性，而TRF冠状体促进了4T1癌细胞的摄取、活性氧的产生和线粒体损伤，从而增强了细胞毒性。这项工作系统地阐明了配体功能化如何协调蛋白质冠结构和随后的蛋白质-细胞级联，为mof的精确生物医学应用提供了机制基础和设计策略。

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引用次数: 0

Engineering pH-Responsive Dendrimer–STAT3 Inhibitor Conjugates for Intracellular Delivery ph响应树突状分子- stat3抑制剂偶联细胞内递送。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01648

Chenikkayala Siva Sankara , Akanksha Ramadas Shanbhag , Daniel Rincón Díaz , Caitlyn A. Hodges , Chenglong Li , Jeffrey K. Harrison , Fan Zhang

Small-molecule STAT3 inhibitors face numerous challenges in clinical translation, including poor water solubility, rapid systemic clearance, low bioavailability, poor selectivity, and high cytotoxicity. To address these limitations, we conjugated the potent STAT3 inhibitor–LLL12 to generate six (G6) hydroxyl-terminated (poly(amidoamine)) PAMAM dendrimers using pH-sensitive linkers: sulfonyl carbamate (carbamate), sulfonyl carbamoyl (amide), and hydrazone for intracellular drug delivery. Conjugation greatly enhanced LLL12 solubility (up to 10 mg/mL) and reduced cytotoxicity without altering dendrimer size or surface charge. All three G6-LLL12 conjugates remained stable under neutral pH but exhibited sustained, pH-dependent drug release correlating with in vitro potency and cytotoxicity. Notably, hydrazone-linked conjugate showed an IC₅₀ = 0.42 ± 0.035 μg/mL, comparable to free LLL12 (IC₅₀ = 0.31 ± 0.05 μg/mL) and superior to amide- and carbamate-linked conjugates. In bone marrow-derived immune suppressive myeloid cells, hydrazone-based G6-LLL12 effectively reduciii-derrive, hydrazone-based G6-LLL12 effectively reduced monocytic myeloid-derived suppressor cell expansion and promoted antigen-presenting cell maturation, highlighting a promising pH-responsive delivery system that enhances solubility and safety while retaining potency.

小分子STAT3抑制剂在临床转化中面临着许多挑战，包括水溶性差、快速全身清除、生物利用度低、选择性差和高细胞毒性。为了解决这些限制，我们偶联了有效的STAT3抑制剂- lll12，使用ph敏感的连接物：磺酰基氨基甲酸酯（氨基甲酸酯）、磺酰基氨基甲酸酯（酰胺）和腙，生成了六个（G6）羟基端（聚（氨基胺））PAMAM树状大分子，用于细胞内药物递送。偶联极大地提高了LLL12的溶解度（高达10 mg/mL），降低了细胞毒性，而不改变树突分子的大小或表面电荷。所有三种G6-LLL12偶联物在中性pH下保持稳定，但表现出持续的pH依赖性药物释放，与体外效力和细胞毒性相关。值得注意的是，腙偶联物的IC50 = 0.42±0.035 μg/mL，与游离LLL12 （IC50 = 0.31±0.05 μg/mL）相当，优于酰胺和氨基甲酸酯偶联物。在骨髓来源的免疫抑制性骨髓细胞中，基于腙的G6-LLL12有效地还原衍生物，基于腙的G6-LLL12有效地减少单核骨髓来源的抑制细胞扩增并促进抗原呈递细胞成熟，突出了一个有前途的ph响应递送系统，在保持效力的同时提高了溶解度和安全性。

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引用次数: 0

An Anti-Inflammatory and Antioxidant Patch Based on Injectable Bioadhesive Hydrogel Prevents Postoperative Atrial Fibrillation 基于可注射生物黏附水凝胶的抗炎抗氧化贴片预防术后房颤。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c01718

Wei-Qi Lu , Ming-Min Zhou , Jin-Qiang Peng , Xiao-Yun Han , Jian-Ling Mo , Jian Ji , Ke-Feng Ren , Xia Sheng

Postoperative atrial fibrillation (POAF) is a common surgical complication linked to atrial inflammation and oxidative stress. Here, we developed a strategy integrating chemical modification of therapeutics, poly(lactic-co-glycolic acid) (PLGA)-mediated sustained kinetics, and hydrogel-enabled spatial targeting to achieve continuous, local drug delivery to the atrial region. Andrographolide was modified with phenylboronic acid (PBAn) to change its solubility and enhance drug-loading capacity in PLGA microspheres, while enabling responsive drug release. We further constructed an injectable bioadhesive hydrogel via mixing of a copolymer and O-carboxymethyl chitosan, which adhered to cardiac tissue. In vitro, PBAn demonstrated ROS-responsive release, along with anti-inflammatory and antioxidant effects on RAW264.7 and HL-1 cells. In a rat pericarditis model, this localized system significantly reduced atrial inflammation and oxidative stress, promoted anti-inflammatory M2 macrophage polarization, enhanced electrical stability, and markedly decreased POAF susceptibility.

术后心房颤动（POAF）是一种常见的手术并发症，与心房炎症和氧化应激有关。在这里，我们开发了一种整合治疗化学修饰，聚乳酸-羟基乙酸（PLGA）介导的持续动力学和水凝胶空间靶向的策略，以实现连续的局部药物递送到心房区域。以苯硼酸（PBAn）修饰穿心莲内酯，改变其溶解度，提高其在PLGA微球中的载药量，同时使药物释放反应灵敏。我们进一步通过共聚物和o -羧甲基壳聚糖的混合构建了可注射的生物胶粘剂水凝胶，并粘附在心脏组织上。在体外，PBAn对RAW264.7和HL-1细胞表现出ros响应性释放，并具有抗炎和抗氧化作用。在大鼠心包炎模型中，该局部系统显著降低心房炎症和氧化应激，促进抗炎M2巨噬细胞极化，增强电稳定性，显著降低POAF易感性。

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引用次数: 0

A Degradable Macromolecular Antioxidant for Efficient Arthritis Treatment 有效治疗关节炎的可降解大分子抗氧化剂。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules

Pub Date : 2026-01-12 DOI: 10.1021/acs.biomac.5c00986

Xianheng Wang , Jie Fang , Lei Yang , Weijie Liu , Li Xiao , Yiwen Li

Reactive oxygen species (ROS) are vital but harmful in excess, and numerous small molecule antioxidants (e.g., polyphenols) can help to maintain an optimal ROS balance. Considering factors like biocompatibility, it is more commonly preferred to transform these natural antioxidants into nanoscavengers through a macromolecular engineering strategy. However, conventional macromolecular antioxidants typically exhibit decreased scavenging capacity compared to monomers due to steric hindrance and oxidation during the polymerization. To tackle this issue, we developed robust degradable macromolecular nanoscavengers by polymerizing dopamine and 4-formylphenylboronic acid. Featuring boronate–catechol linkages, these nanoscavengers boost antioxidation by degrading and exposing functional groups in acidic or ROS environments, showing greater capacity than monomers and polydopamine nanoparticles. Those nanoscavengers were subsequently employed to temporomandibular joint osteoarthritis, suppressing both oxidative stress and inflammation to effectively alleviate the disease progression. This study offers new insights into the design and synthesis of degradable macromolecular antioxidants for inflammation treatment.

活性氧（ROS）是至关重要的，但过量是有害的，许多小分子抗氧化剂（如多酚）可以帮助维持最佳的ROS平衡。考虑到生物相容性等因素，通常更倾向于通过大分子工程策略将这些天然抗氧化剂转化为纳米清除剂。然而，由于空间位阻和聚合过程中的氧化作用，传统的大分子抗氧化剂的清除能力通常低于单体。为了解决这个问题，我们通过聚合多巴胺和4-甲酰苯基硼酸，开发了强大的可降解大分子纳米清除剂。这些纳米清除剂具有硼酸-儿茶酚键，通过降解和暴露在酸性或活性氧环境中的官能团来增强抗氧化能力，表现出比单体和聚多巴胺纳米颗粒更大的能力。这些纳米清除剂随后用于颞下颌关节骨性关节炎，抑制氧化应激和炎症，有效缓解疾病进展。本研究为设计和合成用于炎症治疗的可降解大分子抗氧化剂提供了新的见解。

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引用次数: 0