Background: For elderly people, nocturia has been often found associated with other diseases. Case presentation: The case is 88-year-old male with hypertension, Cardiovascular Disease (CVD), Diabetes, Chronic Kidney Disease (CKD). He developed nocturia and insomnia, and then checked daily water balance by himself. Results: Basic situation showed water intake/urine volume were 750mL/1030mL/day, in which urine ratio of day/night was 27%/73% with waking up twice during night. He was given furosemide 20mg, per os at 1500h. Same study showed 960mL/1090 mL, and urine ratio as 77%/23% with no waking up. Discussion and Conclusion: From some reports, Odds Ratio (OR) for combination of nocturia shows 1.25 for hypertension, 1.23/1.74 for CVD (mild/severe). Consequently, current treatment would contribute adequate managements for nocturia problem.
{"title":"Detail Investigation of Frequent Nocturia and its Improvement by the Administration of Diuretic","authors":"Shimamura Tadao, B. Hiroshi, Takemura Masahiko, Shunto Jouji, Ogawa Hiroko, Nakanishi Miwako, Watanabe Osami, Nagahiro Shinji","doi":"10.33805/2690-2613.125","DOIUrl":"https://doi.org/10.33805/2690-2613.125","url":null,"abstract":"Background: For elderly people, nocturia has been often found associated with other diseases. Case presentation: The case is 88-year-old male with hypertension, Cardiovascular Disease (CVD), Diabetes, Chronic Kidney Disease (CKD). He developed nocturia and insomnia, and then checked daily water balance by himself. Results: Basic situation showed water intake/urine volume were 750mL/1030mL/day, in which urine ratio of day/night was 27%/73% with waking up twice during night. He was given furosemide 20mg, per os at 1500h. Same study showed 960mL/1090 mL, and urine ratio as 77%/23% with no waking up. Discussion and Conclusion: From some reports, Odds Ratio (OR) for combination of nocturia shows 1.25 for hypertension, 1.23/1.74 for CVD (mild/severe). Consequently, current treatment would contribute adequate managements for nocturia problem.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129483910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aging societies have been observed in Japan and other developed countries worldwide. In the light of anti-aging medicine, authors have continued several approaches such as Integrative Medicine (IM), music therapy, art therapy, frailty, rehabilitation, sports medicine and masters’ athletics. For successful aging, three important factors are necessary, which are i) no illness or disability, to maintain physical/cognitive function, to continue social participation and contribution. Music has large power similar to language and communication and clinical effects physically, psychologically and socially. Various plays of the activities for elderly such as music and sports can bring processes towards achieving successful aging.
{"title":"Perspectives of Music and Sports for Satisfactory Anti-Aging Daily Life","authors":"Takenaka Yuko, Konoike Seiji, B. Hiroshi","doi":"10.33805/2690-2613.124","DOIUrl":"https://doi.org/10.33805/2690-2613.124","url":null,"abstract":"Aging societies have been observed in Japan and other developed countries worldwide. In the light of anti-aging medicine, authors have continued several approaches such as Integrative Medicine (IM), music therapy, art therapy, frailty, rehabilitation, sports medicine and masters’ athletics. For successful aging, three important factors are necessary, which are i) no illness or disability, to maintain physical/cognitive function, to continue social participation and contribution. Music has large power similar to language and communication and clinical effects physically, psychologically and socially. Various plays of the activities for elderly such as music and sports can bring processes towards achieving successful aging.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114056704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Duarte de Brito Tiago Marçal Pedro, P. Maria, Machado Humberto
Introduction: Failure to Rescue (FTR) is the failure to prevent a patient’s death after a complication. It measures the ability of a hospital to prevent the death of patients who develop one or more complication that was not present at the time of admission. Therefore, the aim of this study is to review the factors that contribute to FTR, and the measures and strategies that can be applied to prevent the FTR events, in order to discuss the best way to improve patient outcomes in the hospital setting. Methods: A search was conducted on PUBMED retrieving a total of 464 articles. A review of the selected articles’ bibliography was conducted to find other relevant articles. Sixty studies were reviewed in this paper. Results: Patient factors as increasing age, comorbidities and frailty increase the risk of FTR, as well as an increasing number of complications. Several hospital factors, nursing care, and microsystem also influence FTR. Some track and Trigger Systems (TTS) and Early Warning Scores (EWS) have been shown to predict clinical deterioration. On the other hand, machine learning systems have outperformed EWS. Rapid response teams have become the standard approach to delivery and escalation of care, and cognitive aids and crisis checklists also have potential to help reduce FTR. Conclusion: Patient and hospital factors are often non-modifiable; thus, microsystem factors could be a target for improvement. Creating clinical pathways can improve surveillance, and communication tools like SBAR can help relay information. EWS, machine learning models and continuous monitoring are strategies that can help detect clinical deterioration. In the efferent limb rapid response teams have shown to reduce FTR.
{"title":"Failure To Rescue, What Can Be Done To Prevent It?","authors":"Duarte de Brito Tiago Marçal Pedro, P. Maria, Machado Humberto","doi":"10.33805/2690-2613.123","DOIUrl":"https://doi.org/10.33805/2690-2613.123","url":null,"abstract":"Introduction: Failure to Rescue (FTR) is the failure to prevent a patient’s death after a complication. It measures the ability of a hospital to prevent the death of patients who develop one or more complication that was not present at the time of admission. Therefore, the aim of this study is to review the factors that contribute to FTR, and the measures and strategies that can be applied to prevent the FTR events, in order to discuss the best way to improve patient outcomes in the hospital setting. Methods: A search was conducted on PUBMED retrieving a total of 464 articles. A review of the selected articles’ bibliography was conducted to find other relevant articles. Sixty studies were reviewed in this paper.\u0000Results: Patient factors as increasing age, comorbidities and frailty increase the risk of FTR, as well as an increasing number of complications. Several hospital factors, nursing care, and microsystem also influence FTR. Some track and Trigger Systems (TTS) and Early Warning Scores (EWS) have been shown to predict clinical deterioration. On the other hand, machine learning systems have outperformed EWS. Rapid response teams have become the standard approach to delivery and escalation of care, and cognitive aids and crisis checklists also have potential to help reduce FTR. Conclusion: Patient and hospital factors are often non-modifiable; thus, microsystem factors could be a target for improvement. Creating clinical pathways can improve surveillance, and communication tools like SBAR can help relay information. EWS, machine learning models and continuous monitoring are strategies that can help detect clinical deterioration. In the efferent limb rapid response teams have shown to reduce FTR.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123348480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chen Zong-Sian, Trejaut Jean, Loo Jun-Hun, Lai Ying-Hui, Huang Jin-yuan, Lin Marie
This study investigates the genetic relationship of the Mazu peoples on the east coast of China in the Taiwan Strait. Using partial and complete mitochondrial DNA (mtDNA) sequences, we compare Mazu with surrounding East Asia populations. Mazu shows no exclusive affinities with either Southeast or Northeast Asia. High genetic diversity and a very high number of exclusive haplogroups of various Asian origins suggest that Mazu resulted from a process of continuous resettlement that started when it first became an archipelago at the end of the last glacial maximum and that continued till the last century. As a result, genetic drift did not contribute to an exclusive Mazu profile. The structure of haplogroups that show signatures of the Neolithic era (N9a10a), or influx from Island Southeast Asia (F1a4a) suggest recent gene flows and Mazu relationship with it's pre-Neolithic era (presence of pre-E1a or R9/pre-F from Liangdao man) was not seen.
{"title":"Mitochondrial DNA Diversity of the Nangan Islanders Living in the Mazu Archipelago of the Taiwan Strait","authors":"Chen Zong-Sian, Trejaut Jean, Loo Jun-Hun, Lai Ying-Hui, Huang Jin-yuan, Lin Marie","doi":"10.33805/2690-2613.121","DOIUrl":"https://doi.org/10.33805/2690-2613.121","url":null,"abstract":"This study investigates the genetic relationship of the Mazu peoples on the east coast of China in the Taiwan Strait. Using partial and complete mitochondrial DNA (mtDNA) sequences, we compare Mazu with surrounding East Asia populations. Mazu shows no exclusive affinities with either Southeast or Northeast Asia. High genetic diversity and a very high number of exclusive haplogroups of various Asian origins suggest that Mazu resulted from a process of continuous resettlement that started when it first became an archipelago at the end of the last glacial maximum and that continued till the last century. As a result, genetic drift did not contribute to an exclusive Mazu profile. The structure of haplogroups that show signatures of the Neolithic era (N9a10a), or influx from Island Southeast Asia (F1a4a) suggest recent gene flows and Mazu relationship with it's pre-Neolithic era (presence of pre-E1a or R9/pre-F from Liangdao man) was not seen.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127402005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As to the development of treatment for diabetes, Continuous Glucose Monitoring (CGM) has been recently prevalent rapidly. By the analysis of real-time CGM, Ambulatory Glucose Profile (AGP) has been used. It includes time in range (TIR, 70-180 mg/dL), time above range (TAR, >181mg/dL), time below range (TBR, <69 mg/dL), Glycemic Variability (GV), Glucose Management Indicator (GMI), Glycemic variability, Coefficient Of Variation (CV%) and so on. TIR value indicating approximately 70% seems to correlate closely with the HbA1c level of 6.77.0%. Marked discordance of HbA1c values has been found between laboratory HbA1c and estimated HbA1c (eA1c) using GMI from CGM.
{"title":"The Importance of Time in Range (TIR) for Continuous Glucose Monitoring (CGM) in the Clinical Practice for Diabetes","authors":"H. Bando","doi":"10.33805/2690-2613.119","DOIUrl":"https://doi.org/10.33805/2690-2613.119","url":null,"abstract":"As to the development of treatment for diabetes, Continuous Glucose Monitoring (CGM) has been recently prevalent rapidly. By the analysis of real-time CGM, Ambulatory Glucose Profile (AGP) has been used. It includes time in range (TIR, 70-180 mg/dL), time above range (TAR, >181mg/dL), time below range (TBR, <69 mg/dL), Glycemic Variability (GV), Glucose Management Indicator (GMI), Glycemic variability, Coefficient Of Variation (CV%) and so on. TIR value indicating approximately 70% seems to correlate closely with the HbA1c level of 6.77.0%. Marked discordance of HbA1c values has been found between laboratory HbA1c and estimated HbA1c (eA1c) using GMI from CGM.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124436601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rationale: A novel COVID-19 with a severe acute respiratory syndrome or pneumonia had arisen in Wuhan, China in December 2019. Emerging atrial fibrillation in COVID-19 patients is highly significant in cardiovascular medicine. A newly coronary artery spasm in the presentation of COVID-19 infection has certainly a risk impact on both morbidity and mortality of COVID-19 patients. Wavy triple an electrocardiographic sign (Yasser Sign) is an innovated sign of hypocalcaemia linked to tachypnea and acute respiratory distress. Patient concerns: An elderly male COVID-19 patient presented to physician outpatient clinic with bilateral pneumonia, atrial fibrillation, evidence of coronary artery spasm, and Wavy triple an electrocardiographic sign (Yasser Sign). Diagnosis: COVID-19 pneumonia with coronary artery spasm and the Wavy triple an electrocardiographic sign (Yasser Sign). Interventions: Chest CT scan, electrocardiography, oxygenation, and echocardiography. Outcomes: Gradual dramatic clinical, electrocardiographic, and radiological improvement had happened. Lessons: The reversal of electrocardiographic ST-segment depressions in a COVID-19 patient after adding oral nitroglycerine is an indicator for the presence of coronary artery spasm. It signifies the role of the anti-infective drugs, anticoagulants, antiplatelet, and steroids in COVID-19 patients with bilateral pneumonia, AF, coronary artery spasm are effective therapies. The disappearance of AF after initial therapy may a guide for a good prognosis in this case study. The evanescence of Wavy triple ECG sign as a hallmark for the existence of the Movable-weaning phenomenon of hypocalcaemia is recommended for further wide-study.
{"title":"COVID-19 Pneumonia with Atrial Fibrillation, Coronary Spasm, and Wavy Triple Sign (Yasser’s Sign); Dramatic Reversal at Home Management","authors":"Y. Elsayed","doi":"10.33805/2639.6807.130","DOIUrl":"https://doi.org/10.33805/2639.6807.130","url":null,"abstract":"Rationale: A novel COVID-19 with a severe acute respiratory syndrome or pneumonia had arisen in Wuhan, China in December 2019. Emerging atrial fibrillation in COVID-19 patients is highly significant in cardiovascular medicine. A newly coronary artery spasm in the presentation of COVID-19 infection has certainly a risk impact on both morbidity and mortality of COVID-19 patients. Wavy triple an electrocardiographic sign (Yasser Sign) is an innovated sign of hypocalcaemia linked to tachypnea and acute respiratory distress.\u0000Patient concerns: An elderly male COVID-19 patient presented to physician outpatient clinic with bilateral pneumonia, atrial fibrillation, evidence of coronary artery spasm, and Wavy triple an electrocardiographic sign (Yasser Sign). \u0000Diagnosis: COVID-19 pneumonia with coronary artery spasm and the Wavy triple an electrocardiographic sign (Yasser Sign). \u0000Interventions: Chest CT scan, electrocardiography, oxygenation, and echocardiography. \u0000Outcomes: Gradual dramatic clinical, electrocardiographic, and radiological improvement had happened. \u0000Lessons: The reversal of electrocardiographic ST-segment depressions in a COVID-19 patient after adding oral nitroglycerine is an indicator for the presence of coronary artery spasm. It signifies the role of the anti-infective drugs, anticoagulants, antiplatelet, and steroids in COVID-19 patients with bilateral pneumonia, AF, coronary artery spasm are effective therapies. The disappearance of AF after initial therapy may a guide for a good prognosis in this case study. The evanescence of Wavy triple ECG sign as a hallmark for the existence of the Movable-weaning phenomenon of hypocalcaemia is recommended for further wide-study.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129893936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nagahiro, Kei Tanaka, H. Bando, M. Bando, Maki Nishizaki, Satoko Goto
Background: The authors and colleagues have been involved in treatment and care for the elderly with rehabilitation, Integrative Medicine (IM), art in hospitals for artworks using masking tape. Combined together, we conducted a pilot study for medical application of masking tape art-work for elderly subjects. Subjects and Methods: Subjects enrolled were 12 elderly patients with dementia regularly coming to day care center for rehabilitation (M/F 3/9, 78-100 years, 88.3±6.0 years). As methods, masking tape has been known as Washi Tape (wa=Japanese and shi=paper), which is adhesive tape easily to stick and peel off for decorative work. Protocol included i) working 2-3 times a week, ii) sticking masking tape on a rough sketch paper, iii) evaluation of several biomarkers before and after the intervention. Results: i) Artworks were performed satisfactory for each case, ii) grasping power (right) showed increasing tendency from 13.5±7.7kg to 14.8±6.8kg (p=0.11), iii) vitality test showed significantly increase (p=0.02). Discussion and Conclusion: Artworks of masking tape seem to be beneficial for improving grasping power and psychological condition. This study included other biomarkers such as Mini-Mental State Examination (MMSE), pinch power, language word test and Barthel index. We plan to develop further detail analyses in the future research.
{"title":"Masking Tape Art-Work May Provide Beneficial Positive Effects","authors":"S. Nagahiro, Kei Tanaka, H. Bando, M. Bando, Maki Nishizaki, Satoko Goto","doi":"10.33805/2690-2613.117","DOIUrl":"https://doi.org/10.33805/2690-2613.117","url":null,"abstract":"Background: The authors and colleagues have been involved in treatment and care for the elderly with rehabilitation, Integrative Medicine (IM), art in hospitals for artworks using masking tape. Combined together, we conducted a pilot study for medical application of masking tape art-work for elderly subjects. Subjects and Methods: Subjects enrolled were 12 elderly patients with dementia regularly coming to day care center for rehabilitation (M/F 3/9, 78-100 years, 88.3±6.0 years). As methods, masking tape has been known as Washi Tape (wa=Japanese and shi=paper), which is adhesive tape easily to stick and peel off for decorative work. Protocol included i) working 2-3 times a week, ii) sticking masking tape on a rough sketch paper, iii) evaluation of several biomarkers before and after the intervention. Results: i) Artworks were performed satisfactory for each case, ii) grasping power (right) showed increasing tendency from 13.5±7.7kg to 14.8±6.8kg (p=0.11), iii) vitality test showed significantly increase (p=0.02). Discussion and Conclusion: Artworks of masking tape seem to be beneficial for improving grasping power and psychological condition. This study included other biomarkers such as Mini-Mental State Examination (MMSE), pinch power, language word test and Barthel index. We plan to develop further detail analyses in the future research.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132670667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Shimamura, H. Bando, S. Nagahiro, M. Nakanishi, Osami Watanabe
Case: The case is 86-year-old male hypertensive patient with anti-hypertensive drug for 5 years. He has been provided Amlodipine besilate 5mg at 0800h and doxazosin mesylate 2mg at 2300h for long. In June 2020, he noticed unstable fluctuation of Blood Pressure (BP) during morning, afternoon and night. Results: Then, he checked the circadian rhythm of BP, which showed higher BP in early morning, decreasing BP 0800-1000h, minimum BP during 1000-1400h, increasing BP during 1400-1800h and stable BP during 1800-2400h. Due to the result, he changed to take amlodipine at 2300h. Consequently, his BP gradually became stable during 24 hours after 2 weeks. Discussion: Some factors may exist for contributing improved BP fluctuation. They include a) pathophysiological characteristics of BP circadian rhythm, b) effective time for anti-hypertensive drug, c) accuracy of the obtained BP data and d) the social and psychological reliability of the patient. Regarding d) he was engaged in research and development work as a senior researcher at a chemical company. He has been also a member of New Elderly Association (NEA), which was established by Shigeaki Hinohara. He lives on the philosophy of Hinohara-ism for long, associated with stable mind and body.
{"title":"Improved Hypertension by Investigating Circadian Rhythm of Blood Pressure","authors":"T. Shimamura, H. Bando, S. Nagahiro, M. Nakanishi, Osami Watanabe","doi":"10.33805/2690-2613.116","DOIUrl":"https://doi.org/10.33805/2690-2613.116","url":null,"abstract":"Case: The case is 86-year-old male hypertensive patient with anti-hypertensive drug for 5 years. He has been provided Amlodipine besilate 5mg at 0800h and doxazosin mesylate 2mg at 2300h for long. In June 2020, he noticed unstable fluctuation of Blood Pressure (BP) during morning, afternoon and night. Results: Then, he checked the circadian rhythm of BP, which showed higher BP in early morning, decreasing BP 0800-1000h, minimum BP during 1000-1400h, increasing BP during 1400-1800h and stable BP during 1800-2400h. Due to the result, he changed to take amlodipine at 2300h. Consequently, his BP gradually became stable during 24 hours after 2 weeks. Discussion: Some factors may exist for contributing improved BP fluctuation. They include a) pathophysiological characteristics of BP circadian rhythm, b) effective time for anti-hypertensive drug, c) accuracy of the obtained BP data and d) the social and psychological reliability of the patient. Regarding d) he was engaged in research and development work as a senior researcher at a chemical company. He has been also a member of New Elderly Association (NEA), which was established by Shigeaki Hinohara. He lives on the philosophy of Hinohara-ism for long, associated with stable mind and body.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121771128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-09DOI: 10.20944/preprints202001.0068.v1
S. H. Goudarzi, B. Kabat, M. Cannon, M. Gashkoff, Rachel Zurek
This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the Short Chain Fatty Acids (SCFA) by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for SCFA analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973-mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in BHI supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acetic acid, propionic acid, butanoic acid, pentanoic acid, ethyl butaric acid, 4-methylvaleric acid, hexanoic acid. When the BHI broth supplemented with 2% or 10% sucrose containing erythritol was used as media for this Streptococcus mutans strain, the following were produced: ethanol, acetoin, and acetic acid. Our results would indicate that constituents of the bacteria media may affect the bacterial metabolite production.
本实验对变形链球菌ATCC 35668进行了初步研究，在含和不含多元醇(赤藓糖醇)的培养基中生长，通过头部空间分析测量了产生的代谢物，包括短链脂肪酸(SCFA)。脑心灌注肉汤(BHI2或BHI10)添加2%或10%的蔗糖，不含多元醇或赤四糖醇或木糖醇，以及变形链球菌(ATCC 35668)进行有氧培养。生长48小时后，收集上清液并离心成球细菌。从细菌微球中去除上清液，然后用Agilent Technologies (Santa Clara, CA 95051)系统进行SCFA分析，该系统由三个组件组成，5973-质量选择检测器，6890N气相色谱仪和7697A顶空取样器。在添加2%或10%蔗糖但不含多元醇的BHI中生长的变形链球菌产生以下短链脂肪酸:异戊酸甲酯、乙酸、丙酸、丁酸、戊酸、丁酸乙酯、4-甲基戊酸、己酸。将添加2%或10%蔗糖的BHI肉汤作为该变形链球菌菌株的培养基，可产生乙醇、乙糖苷和乙酸。我们的结果表明，细菌培养基的成分可能会影响细菌代谢物的产生。
{"title":"Pilot study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols","authors":"S. H. Goudarzi, B. Kabat, M. Cannon, M. Gashkoff, Rachel Zurek","doi":"10.20944/preprints202001.0068.v1","DOIUrl":"https://doi.org/10.20944/preprints202001.0068.v1","url":null,"abstract":"This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the Short Chain Fatty Acids (SCFA) by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for SCFA analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973-mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in BHI supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acetic acid, propionic acid, butanoic acid, pentanoic acid, ethyl butaric acid, 4-methylvaleric acid, hexanoic acid. When the BHI broth supplemented with 2% or 10% sucrose containing erythritol was used as media for this Streptococcus mutans strain, the following were produced: ethanol, acetoin, and acetic acid. Our results would indicate that constituents of the bacteria media may affect the bacterial metabolite production.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133860481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gloria Phebeni, Nomsa Nxumalo-Magagula, R. N. Mkhonta, Tengetile R. Mathunjwa-Dlamini
Background: In women cervical cancer is the leading cause of death among all cancers in developing countries, but it can be controlled through prevention and early detection of precursor lesions. In 2013 there were 223 new cases of cervical cancer in Swaziland with an estimated 118 cervical cancer related deaths. Most clients suffering from cervical cancer were below the age of 40 years and were diagnosed in the late stage. The study determined knowledge, attitudes and practices of women in relation to cervical cancer screening and treatment at one of the health facilities in the Hhohho Region, in Swaziland. Methodology: A quantitative-descriptive approach was utilized among 56 participants selected using purposive sampling. Respondents were women who came for health care services at the Health Facility’s Antiretroviral Therapy (ART) Department. The collected data were entered into SPSS and analyzed using descriptive statistics and Pearson’s correlation. Findings: Ninety-four percent (94.6%) of the respondents reported to have heard of cervical cancer, and 96.4% reported that screening for cervical cancer could detect symptoms before they appeared. Only 1.8% was aware of the association between cervical cancer and the Human Papilloma Virus (HPV). Thirty-seven percent (37.5%) of the respondents reported to have ever screened for cervical cancer. The major reasons reported for not screening were busy work schedule, and being turned back by nurses. There was a significant relationship between level of education and knowledge of risk factors for cervical cancer (r=0.306, p=0.022). Data also supported a significant relationship between age of the respondents and knowledge on how to protect self from getting cervical cancer(r=-0.402, p=0.002). Data supported a significant relationship between knowledge on risk factors and knowledge on how to protect self from acquiring cervical cancer (r=0.295, p=0.027). Recommendations: It is recommended that nursing practice should also focus on the provision of services to the working class by offering cervical cancer screening services on weekends and public holidays. Nurses need to be more responsive to clients’ health needs and avoid turning clients back.
{"title":"Knowledge, Attitudes and Practices of Women Attending One of the Health Facilities in Hhohho Region, Swaziland, in Relation to Cervical Cancer and Screening","authors":"Gloria Phebeni, Nomsa Nxumalo-Magagula, R. N. Mkhonta, Tengetile R. Mathunjwa-Dlamini","doi":"10.33805/2690-2613.106","DOIUrl":"https://doi.org/10.33805/2690-2613.106","url":null,"abstract":"Background: In women cervical cancer is the leading cause of death among all cancers in developing countries, but it can be controlled through prevention and early detection of precursor lesions. In 2013 there were 223 new cases of cervical cancer in Swaziland with an estimated 118 cervical cancer related deaths. Most clients suffering from cervical cancer were below the age of 40 years and were diagnosed in the late stage. The study determined knowledge, attitudes and practices of women in relation to cervical cancer screening and treatment at one of the health facilities in the Hhohho Region, in Swaziland.\u0000Methodology: A quantitative-descriptive approach was utilized among 56 participants selected using purposive sampling. Respondents were women who came for health care services at the Health Facility’s Antiretroviral Therapy (ART) Department. The collected data were entered into SPSS and analyzed using descriptive statistics and Pearson’s correlation.\u0000Findings: Ninety-four percent (94.6%) of the respondents reported to have heard of cervical cancer, and 96.4% reported that screening for cervical cancer could detect symptoms before they appeared. Only 1.8% was aware of the association between cervical cancer and the Human Papilloma Virus (HPV). Thirty-seven percent (37.5%) of the respondents reported to have ever screened for cervical cancer. The major reasons reported for not screening were busy work schedule, and being turned back by nurses. There was a significant relationship between level of education and knowledge of risk factors for cervical cancer (r=0.306, p=0.022). Data also supported a significant relationship between age of the respondents and knowledge on how to protect self from getting cervical cancer(r=-0.402, p=0.002). Data supported a significant relationship between knowledge on risk factors and knowledge on how to protect self from acquiring cervical cancer (r=0.295, p=0.027).\u0000Recommendations: It is recommended that nursing practice should also focus on the provision of services to the working class by offering cervical cancer screening services on weekends and public holidays. Nurses need to be more responsive to clients’ health needs and avoid turning clients back.","PeriodicalId":315411,"journal":{"name":"Edelweiss Journal of Biomedical Research and Review","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130233111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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