Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.7
Gerry Gunawan, M. Dalhar, S. Kurniawan
Parkinson's disease is a neurodegenerative disorder that is progressive about the movement or control of movement. The disease often occurs in people over the age of 60 years. The etiology of Parkinson's disease caused by a combination of genetic and environmental factors. Because overall life expectancy increases, the number of people with Parkinson's disease will increase in the future. Treatment of Parkinson's disease can be used with pharmacological therapy and nonpharmacological therapy. Pharmacological therapy can use levodopa, monoamine oxidase-B inhibitors, dopamine agonists, anticholinergics and amantadine, while nonpharmacological therapies may use the method of stem cell therapy. Stem cells are master cells that have two important characteristics that can perform self-renewing through cell division and can be induced to become cells with specific functions. The aim of Stem cell therapy in Parkinson's disease to replace the damaged dopaminergic cells.
{"title":"PARKINSON AND STEM CELL THERAPY","authors":"Gerry Gunawan, M. Dalhar, S. Kurniawan","doi":"10.21776/UB.MNJ.2017.003.01.7","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.7","url":null,"abstract":"Parkinson's disease is a neurodegenerative disorder that is progressive about the movement or control of movement. The disease often occurs in people over the age of 60 years. The etiology of Parkinson's disease caused by a combination of genetic and environmental factors. Because overall life expectancy increases, the number of people with Parkinson's disease will increase in the future. Treatment of Parkinson's disease can be used with pharmacological therapy and nonpharmacological therapy. Pharmacological therapy can use levodopa, monoamine oxidase-B inhibitors, dopamine agonists, anticholinergics and amantadine, while nonpharmacological therapies may use the method of stem cell therapy. Stem cells are master cells that have two important characteristics that can perform self-renewing through cell division and can be induced to become cells with specific functions. The aim of Stem cell therapy in Parkinson's disease to replace the damaged dopaminergic cells.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"10 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.1
M. R. Indra, Eko Arisetijono Marhaendraputro, Rudi Rakhmad Hidayat
Background. Alzheimer's disease is a progressive neurologic disease of the brain that triggers irreversible neuronal cell loss, which can interfere with social and occupational functioning. The theory of ACH (Amyloid Cascade Hypothesis) states there are deposits and misfolding of beta amyloid protein thus lead to the formation of plaques and tangles in neurons cells. Objective. To identify the immunogenicity of beta amyloid polyclonal antibodies that can be developed as a first step early diagnosis of Alzheimer's disease. Methods. Rrandomized group post test only design conducted on rabbits. Blood samples were taken from rabbits that had been injected antigen once a week for 5 weeks. Variables were found in this study is the formation of beta amyloid polyclonal antibody with detection levels using dot blot and ELISA methods. Results. It has been reproduced specific polyclonal antibody beta amyloid which has been evidenced by the bond between the antigen with the antibody in a dot blot. Conclusion. The beta amyloid antibodies can be produced through production techniques with a polyclonal antibody against beta amyloid antigen induced rabbit.
{"title":"BETA AMYLOID POLYCLONAL ANTIBODY IMMUNOGENICITY AS EARLY DEVELOPMENT STUDY OF EARLY DIAGNOSIS FOR ALZHEIMER’S DISEASE","authors":"M. R. Indra, Eko Arisetijono Marhaendraputro, Rudi Rakhmad Hidayat","doi":"10.21776/UB.MNJ.2017.003.01.1","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.1","url":null,"abstract":"Background. Alzheimer's disease is a progressive neurologic disease of the brain that triggers irreversible neuronal cell loss, which can interfere with social and occupational functioning. The theory of ACH (Amyloid Cascade Hypothesis) states there are deposits and misfolding of beta amyloid protein thus lead to the formation of plaques and tangles in neurons cells. Objective. To identify the immunogenicity of beta amyloid polyclonal antibodies that can be developed as a first step early diagnosis of Alzheimer's disease. Methods. Rrandomized group post test only design conducted on rabbits. Blood samples were taken from rabbits that had been injected antigen once a week for 5 weeks. Variables were found in this study is the formation of beta amyloid polyclonal antibody with detection levels using dot blot and ELISA methods. Results. It has been reproduced specific polyclonal antibody beta amyloid which has been evidenced by the bond between the antigen with the antibody in a dot blot. Conclusion. The beta amyloid antibodies can be produced through production techniques with a polyclonal antibody against beta amyloid antigen induced rabbit.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.2
M. R. Indra, Zanella Yolanda Lie
Background. Grape peel and seed extract (Vitis vinifera), that has resveratrol, is one of many antioxidants that can pass through blood brain barrier and can induce release neurotrophic factor that contribute in ERK 1/2 pathway mechanism in post stroke. Objective . To prove that grape peel and seed extract can regenerate neuron in brain functional. Methods. True experimental design with five groups in this research. The five groups are negative control, positive control, grape peel and seed extract 50mg/KgBW, 100mg/KgBW, and 200mg/KgBW. rats are given grape peel and seed extract in variable dose to know how extract’s effect in neuron repairment. The repairment is monitored from ladder rung walking test score. Results. Range average score ladder rung walking test post stroke dan post treatment group N, K, Ra, Rb, dan Rc, were 0 ± 0, 0.001028933 ± 0.011664445, 0.123214286 ± 0.019834983, 0.064744427 ± 0.024296721, 0.03781401 ± 0.006888803. Statistical test used Annova significantly p;0,001.Dose 50mg/KgBW is effective in repairing neuron. Conclusion . Grape’s leather and Seed extract 50 mg/kgBW can improve neuron regeneration on animal model.
{"title":"GRAPE’S LEATHER AND SEED EXTRACT (VITIS VINIFERA) IMPROVING THE FUNCTION OF WISTAR RATS’ MOTOR (RATTUS NORVEGICUS) ISCHEMIC STROKE MODEL","authors":"M. R. Indra, Zanella Yolanda Lie","doi":"10.21776/UB.MNJ.2017.003.01.2","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.2","url":null,"abstract":"Background. Grape peel and seed extract (Vitis vinifera), that has resveratrol, is one of many antioxidants that can pass through blood brain barrier and can induce release neurotrophic factor that contribute in ERK 1/2 pathway mechanism in post stroke. Objective . To prove that grape peel and seed extract can regenerate neuron in brain functional. Methods. True experimental design with five groups in this research. The five groups are negative control, positive control, grape peel and seed extract 50mg/KgBW, 100mg/KgBW, and 200mg/KgBW. rats are given grape peel and seed extract in variable dose to know how extract’s effect in neuron repairment. The repairment is monitored from ladder rung walking test score. Results. Range average score ladder rung walking test post stroke dan post treatment group N, K, Ra, Rb, dan Rc, were 0 ± 0, 0.001028933 ± 0.011664445, 0.123214286 ± 0.019834983, 0.064744427 ± 0.024296721, 0.03781401 ± 0.006888803. Statistical test used Annova significantly p;0,001.Dose 50mg/KgBW is effective in repairing neuron. Conclusion . Grape’s leather and Seed extract 50 mg/kgBW can improve neuron regeneration on animal model.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"5-11"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.4
Edwina Narulita Sari Agustin Junaidi, S. Winarsih, Tina Handayani Nasution
Background. Nutritional status is predictor that can affects on clinical outcome in patient with acute phase ischemic stroke. Objective. To investigate the difference of nutritional status level with clinical outcome in patient with acute phase ischemic stroke. Methods. Cohort retrospective design used medical record of all registered acute ischemic stroke patient in hospital Dr. Saiful Anwar Malang. 60 sample that inquired inclusion criteria and classified into three groups (the group of patient with acute phase ischemic stroke with normal nutrition status, undernutrition status, and overnutrition status). Nutritional status measured with body mass index parameter and clinical outcome measured with of NIHSS score admission and NIHSS score discharge. Results . There were 60 had good clinical outcome with undernutrition status (n=20) amount 65%, normal nutrition status (n=20) amount 70% and overnutrition status (n=20) amount 60%. The difference of nutritional status level with clininical outcome in patient with acute phase ischemic stroke showed that no significantly difference (chi square, p-value=0,803). Conclusion. There is no difference of nutritional status level with clinical outcome in patient with acute phase ischemic stroke.
{"title":"THE DIFFERENCE LEVEL OF NUTRITIONAL STATUS WITH CLINICAL OUTCOME IN PATIENT WITH ACUTE PHASE ISCHEMIC STROKE","authors":"Edwina Narulita Sari Agustin Junaidi, S. Winarsih, Tina Handayani Nasution","doi":"10.21776/UB.MNJ.2017.003.01.4","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.4","url":null,"abstract":"Background. Nutritional status is predictor that can affects on clinical outcome in patient with acute phase ischemic stroke. Objective. To investigate the difference of nutritional status level with clinical outcome in patient with acute phase ischemic stroke. Methods. Cohort retrospective design used medical record of all registered acute ischemic stroke patient in hospital Dr. Saiful Anwar Malang. 60 sample that inquired inclusion criteria and classified into three groups (the group of patient with acute phase ischemic stroke with normal nutrition status, undernutrition status, and overnutrition status). Nutritional status measured with body mass index parameter and clinical outcome measured with of NIHSS score admission and NIHSS score discharge. Results . There were 60 had good clinical outcome with undernutrition status (n=20) amount 65%, normal nutrition status (n=20) amount 70% and overnutrition status (n=20) amount 60%. The difference of nutritional status level with clininical outcome in patient with acute phase ischemic stroke showed that no significantly difference (chi square, p-value=0,803). Conclusion. There is no difference of nutritional status level with clinical outcome in patient with acute phase ischemic stroke.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.6
Fathia Annis Pramesti, M. Husna, S. Kurniawan, Masruroh Rahayu
Status epilepticus is an emergency condition in the field of neurology are often undiagnosed and are associated with high mortality and long-term disability. One type of status epilepticus is nonconvulsive status epilepticus (NCSE) in which the diagnosis of NCSE is very difficult because the clinical manifestations appear is agitation or confusion, nystagmus or bizarre behavior such as lip smacking or take goods in the air. The diagnosis was based on clinical features, especially the mental status or the disrupted of consciousness and the changes in the EEG. Diagnosis of NCSE is an important first step, which can avoid the delay in therapy in order to prevent irreversible brain damage. Treatment is by administering benzodiazepines and antiepileptic drugs, while the prognosis is determined by the etiology and associated with brain damage there.
{"title":"DIAGNOSIS AND MANAGEMENT OF NONCONVULSIVE STATUS EPILEPTICUS (NCSE)","authors":"Fathia Annis Pramesti, M. Husna, S. Kurniawan, Masruroh Rahayu","doi":"10.21776/UB.MNJ.2017.003.01.6","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.6","url":null,"abstract":"Status epilepticus is an emergency condition in the field of neurology are often undiagnosed and are associated with high mortality and long-term disability. One type of status epilepticus is nonconvulsive status epilepticus (NCSE) in which the diagnosis of NCSE is very difficult because the clinical manifestations appear is agitation or confusion, nystagmus or bizarre behavior such as lip smacking or take goods in the air. The diagnosis was based on clinical features, especially the mental status or the disrupted of consciousness and the changes in the EEG. Diagnosis of NCSE is an important first step, which can avoid the delay in therapy in order to prevent irreversible brain damage. Treatment is by administering benzodiazepines and antiepileptic drugs, while the prognosis is determined by the etiology and associated with brain damage there.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"30-38"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.5
Rania Arif Mahfud, D. Lyrawati, Imam Sarwono
Background. Diabetic neuropaty is a condition that can affect pyramidal cells and neuronal cells in the hippocampus. Alpha lipoic acid is effective in pathological conditions where ROS (Reactive Oxygen Species) have been implicated, include in brain. Objective. To investigate effects of ALA on oxidative stress in diabetic brain of male Wistar rats. Methods. True experimental design and Posttest Only Control Group are used in this study. Thirty male rats were randomly divided into 5 groups: normal rats without ALA (NTA), diabetic rats without ALA (DTA), diabetic rats with ALA 80 mg, ALA dose 200 mg, and ALA dose 500 mg/kg/day. ALA therapy in mice conducted orally once a day. Diabetes was induced in rats by single intraperitonial injection of streptozotocin (STZ) at 60 mg/kg body weight. The content of malondialdehyde (MDA) in the brain was measured by spectrophotometeric assays. Brain structure (pyramidal cell in hippocampus) was assessed by staining with hematoxylin and eosin. Results. MDA levels in the DTA, DA80 and DA200 is greater than the levels of MDA in the NTA group, but not statistically significant at the MDA values (p = 0,260). Test-Product Moment Pearson correlation showed a weak positive relationship and not significant (r = 0,327) between the groups of NTA, DA80 and DA200 with MDA. No differences pyramidal cell structure between NTA and DTA. Conclusion. The treatment for 4 weeks with ALA had not reduced oxidative stress in diabetic brain.
{"title":"EFFECT OF ALPHA LIPOIC ACID ON MDA LEVELS AND HISTOLOGY OF BRAIN IN TYPE 1 DM","authors":"Rania Arif Mahfud, D. Lyrawati, Imam Sarwono","doi":"10.21776/UB.MNJ.2017.003.01.5","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.5","url":null,"abstract":"Background. Diabetic neuropaty is a condition that can affect pyramidal cells and neuronal cells in the hippocampus. Alpha lipoic acid is effective in pathological conditions where ROS (Reactive Oxygen Species) have been implicated, include in brain. Objective. To investigate effects of ALA on oxidative stress in diabetic brain of male Wistar rats. Methods. True experimental design and Posttest Only Control Group are used in this study. Thirty male rats were randomly divided into 5 groups: normal rats without ALA (NTA), diabetic rats without ALA (DTA), diabetic rats with ALA 80 mg, ALA dose 200 mg, and ALA dose 500 mg/kg/day. ALA therapy in mice conducted orally once a day. Diabetes was induced in rats by single intraperitonial injection of streptozotocin (STZ) at 60 mg/kg body weight. The content of malondialdehyde (MDA) in the brain was measured by spectrophotometeric assays. Brain structure (pyramidal cell in hippocampus) was assessed by staining with hematoxylin and eosin. Results. MDA levels in the DTA, DA80 and DA200 is greater than the levels of MDA in the NTA group, but not statistically significant at the MDA values (p = 0,260). Test-Product Moment Pearson correlation showed a weak positive relationship and not significant (r = 0,327) between the groups of NTA, DA80 and DA200 with MDA. No differences pyramidal cell structure between NTA and DTA. Conclusion. The treatment for 4 weeks with ALA had not reduced oxidative stress in diabetic brain.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"23-29"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.21776/UB.MNJ.2017.003.01.3
G. Santoso, H. Sujuti, D. Hidayati
Background. Tuberculosis caused by mycobacterium tuberculosis. The support of these inflamated factors contained in this focus determine the prognosis of TB towards Central Nervous System. One of the inflammation factors is TNF-α. Objective. To find the expression of TNF-α on the brain which are infected by M. tuberculosis. Methods. Experimental study, Wild Mus musculus to compare the group which has got infected before. The similiar study has been conducted by Laksmi Wulandari, pulmonologist. The observation towards the expression of TNF-a into a mouse’s brain was done by using the imunohistokimia method. It is marked by the brown color in the core, sitoplasma and the cell with upgrading size 100x times 20. Results. This is evident that significanly corelation (P = 0.000) between independent Mycobacterium tuberculosis infection at incubation periode 8 and 16 weeks) and dependent variabel (TNF-α expression on brain winstar tissue that infected by Mycobacterium tuberculosis for 8 and 16 weeks). Conclusion. Strong relationship between expression of TNF-a and the M. Tuberculosis’ infection.
{"title":"THE EFFECT OF INFECTION OF MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV TOWARDS THE EXPRESSION OF TNF- α IN THE BRAIN","authors":"G. Santoso, H. Sujuti, D. Hidayati","doi":"10.21776/UB.MNJ.2017.003.01.3","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2017.003.01.3","url":null,"abstract":"Background. Tuberculosis caused by mycobacterium tuberculosis. The support of these inflamated factors contained in this focus determine the prognosis of TB towards Central Nervous System. One of the inflammation factors is TNF-α. Objective. To find the expression of TNF-α on the brain which are infected by M. tuberculosis. Methods. Experimental study, Wild Mus musculus to compare the group which has got infected before. The similiar study has been conducted by Laksmi Wulandari, pulmonologist. The observation towards the expression of TNF-a into a mouse’s brain was done by using the imunohistokimia method. It is marked by the brown color in the core, sitoplasma and the cell with upgrading size 100x times 20. Results. This is evident that significanly corelation (P = 0.000) between independent Mycobacterium tuberculosis infection at incubation periode 8 and 16 weeks) and dependent variabel (TNF-α expression on brain winstar tissue that infected by Mycobacterium tuberculosis for 8 and 16 weeks). Conclusion. Strong relationship between expression of TNF-a and the M. Tuberculosis’ infection.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"3 1","pages":"12-16"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-27DOI: 10.21776/UB.MNJ.2016.002.02.5
Brigitta Ida, F. Nugroho, Inke Triana Arysanthi
Background. Breastfeeding is an important activity to maintain and prepare the next generation in the future. The low of exclusive breastfeeding at the family is one of the triggers for low of nutritional status and it associated with neurodevelopmental delays of infants. Objective. To identify and analyze the correlation of nutritional status on neurodevelopmental development in infants aged 0-6 months who are exclusive and non-exclusive breastfeeding in Kedungkandang Health Center Malang. Methods. An observational analytic using a cross-sectional approach . The samples were taken by Consecutive Sampling technique. Data were analyzed by Spearman Rank correlation test. Results. There is a significant correlation between nutritional status with neurodevelopmental development in infants aged 0-6 months with significance value p = 0.000 ( 0.000 < 0.05 ) and the strength of correlation (r=0,552) is medium. Conclusion. There is a positive correlation between nutritional status with neurodevelopmental progress of infants aged 0-6 months who are exclusively breastfed . Exclusive breastfeeding affects the nutritional status better, then a good nutritional status affected neurodevelopmental development also better.
{"title":"NUTRITIONAL STATUS RELATION TOWARD DEVELOPMENT OF NEURODEVELOPMENTAL TO INFANTS AGED 0-6 MONTHS WHO RECEIVED BREAST MILK EXCLUSIVELY AND NONEXCLUSIVELY (IN PUSKESMAS KEDUNGKANDANG MALANG)","authors":"Brigitta Ida, F. Nugroho, Inke Triana Arysanthi","doi":"10.21776/UB.MNJ.2016.002.02.5","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2016.002.02.5","url":null,"abstract":"Background. Breastfeeding is an important activity to maintain and prepare the next generation in the future. The low of exclusive breastfeeding at the family is one of the triggers for low of nutritional status and it associated with neurodevelopmental delays of infants. Objective. To identify and analyze the correlation of nutritional status on neurodevelopmental development in infants aged 0-6 months who are exclusive and non-exclusive breastfeeding in Kedungkandang Health Center Malang. Methods. An observational analytic using a cross-sectional approach . The samples were taken by Consecutive Sampling technique. Data were analyzed by Spearman Rank correlation test. Results. There is a significant correlation between nutritional status with neurodevelopmental development in infants aged 0-6 months with significance value p = 0.000 ( 0.000 < 0.05 ) and the strength of correlation (r=0,552) is medium. Conclusion. There is a positive correlation between nutritional status with neurodevelopmental progress of infants aged 0-6 months who are exclusively breastfed . Exclusive breastfeeding affects the nutritional status better, then a good nutritional status affected neurodevelopmental development also better.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"2 1","pages":"71-78"},"PeriodicalIF":0.0,"publicationDate":"2016-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-27DOI: 10.21776/UB.MNJ.2016.002.02.2
Rima Nor Kaspia, D. Hidayati, Aris Widayati
Background. Ischemia on the cells and the lack of supply of glucose, will trigger a Ca2 + influx into the cell and increased expression of glutamate. Result in mitochondrial Ca2 + Influx be "overloaded". Glucose metabolism then switched to the anaerobic process that makes ATP increasingly depleted and there acidosis. This situation makes the neuron cell apoptosis triggered to occur. Apoptosis is programmed cell death. Neuron cell apoptosis is thought to have a strong connection to the tuberculosis infection in the brain. Objective. To determine the number of cells undergoing apoptosis neurons in brain tissue of mice. Methods. This study is a semiquantitative by comparing the number of cells undergoing apoptosis neurons in the three groups of samples. Observations apoptosis of neuronal cells in the brain tissue of mice was conducted using TUNEL staining technique (terminal deoxynucleotidyl transferase dUTP nick end labeling) are seen in a microscope with a magnification of 1000x. Results. The results showed that neuronal cells undergo apoptosis in brain tissue infected with M.tb for 8 and 16 weeks were marked with brown color in the cell nucleus. Neuron cell apoptosis were observed at M.tb-infected brain tissue for 16 weeks. Conclusion. The longer the M.tb infection can affect the increase in the number of neuronal cell apoptosis.
背景。细胞缺血和葡萄糖供应不足，会触发Ca2 +内流进入细胞，谷氨酸表达增加。导致线粒体Ca2 +内流“超载”。葡萄糖代谢随后转变为厌氧过程，使ATP逐渐耗尽，并导致酸中毒。这种情况使神经元细胞凋亡触发发生。细胞凋亡是细胞程序性死亡。神经元细胞凋亡被认为与脑结核感染有密切联系。目标。目的:测定小鼠脑组织中发生凋亡的神经元细胞数量。方法。本研究采用半定量方法，比较三组样品中发生凋亡神经元的细胞数量。用TUNEL染色技术(末端脱氧核苷酸转移酶dUTP nick end labeling)观察小鼠脑组织神经元细胞凋亡，显微镜放大1000倍。结果。结果表明，感染结核分枝杆菌8周和16周的脑组织神经元细胞发生凋亡，细胞核呈棕色。结核分枝杆菌感染16周后观察脑组织神经元细胞凋亡。结论。结核分枝杆菌感染时间越长，可影响神经元细胞凋亡数量的增加。
{"title":"THE INFECTION EFFECT OF STRAIN H37Rv MYCOBACTERIUM TUBERCULOSIS ON APOPTOSIS OF MICE’S NEURON CELL BRAIN (MUS MUSCULUS)","authors":"Rima Nor Kaspia, D. Hidayati, Aris Widayati","doi":"10.21776/UB.MNJ.2016.002.02.2","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2016.002.02.2","url":null,"abstract":"Background. Ischemia on the cells and the lack of supply of glucose, will trigger a Ca2 + influx into the cell and increased expression of glutamate. Result in mitochondrial Ca2 + Influx be \"overloaded\". Glucose metabolism then switched to the anaerobic process that makes ATP increasingly depleted and there acidosis. This situation makes the neuron cell apoptosis triggered to occur. Apoptosis is programmed cell death. Neuron cell apoptosis is thought to have a strong connection to the tuberculosis infection in the brain. Objective. To determine the number of cells undergoing apoptosis neurons in brain tissue of mice. Methods. This study is a semiquantitative by comparing the number of cells undergoing apoptosis neurons in the three groups of samples. Observations apoptosis of neuronal cells in the brain tissue of mice was conducted using TUNEL staining technique (terminal deoxynucleotidyl transferase dUTP nick end labeling) are seen in a microscope with a magnification of 1000x. Results. The results showed that neuronal cells undergo apoptosis in brain tissue infected with M.tb for 8 and 16 weeks were marked with brown color in the cell nucleus. Neuron cell apoptosis were observed at M.tb-infected brain tissue for 16 weeks. Conclusion. The longer the M.tb infection can affect the increase in the number of neuronal cell apoptosis.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"2 1","pages":"52-59"},"PeriodicalIF":0.0,"publicationDate":"2016-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68323977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-27DOI: 10.21776/UB.MNJ.2016.002.02.6
M. Husna, Bowo Hery Prasetyo
Malaria is still becoming a health problem in the world and in Indonesia. Cerebral malaria is one of many features of severe and life threatening malaria. Many hypotheses underlying the pathophysiology of cerebral malaria have been disclosed, but the one that evolved nowdays is the hyphotesis of mechanical, permeability, humoral, and MMPs. These hypotheses proposed about biomolecular aspects of cerebral malaria and the mechanism is still not well understood. The understanding of this pathophysiology will aid the treatment of cerebral malaria. Current basic treatment of cerebral malaria is the ACT (artemisinin base combination treatment) drugs, supportive treatment and the management of its complications which is indispensable. Many research has been conducted and still in progress for finding the best optimal treatment.
{"title":"BIOMOLECULAR ASPECTS AND UPDATE ON TREATMENT OF CEREBRAL MALARIA","authors":"M. Husna, Bowo Hery Prasetyo","doi":"10.21776/UB.MNJ.2016.002.02.6","DOIUrl":"https://doi.org/10.21776/UB.MNJ.2016.002.02.6","url":null,"abstract":"Malaria is still becoming a health problem in the world and in Indonesia. Cerebral malaria is one of many features of severe and life threatening malaria. Many hypotheses underlying the pathophysiology of cerebral malaria have been disclosed, but the one that evolved nowdays is the hyphotesis of mechanical, permeability, humoral, and MMPs. These hypotheses proposed about biomolecular aspects of cerebral malaria and the mechanism is still not well understood. The understanding of this pathophysiology will aid the treatment of cerebral malaria. Current basic treatment of cerebral malaria is the ACT (artemisinin base combination treatment) drugs, supportive treatment and the management of its complications which is indispensable. Many research has been conducted and still in progress for finding the best optimal treatment.","PeriodicalId":31552,"journal":{"name":"Malang Neurology Journal","volume":"2 1","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2016-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68324363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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