Nickel (Ni) is an essential microelement for higher plants and an important one for many other living organisms. It is present in every part of the biosphere. Higher plants have small requirements towards Ni for their optimal growth and development. That is why its latent and acute deficiencies in nature rarely occur. Soil and water pollution with Ni is a global issue. This is the reason special attention is paid to the effects of higher Ni concentrations on plants metabolism, their organic production, as well as on the possibility of using plants for phytoremediation of Ni contaminated habitats. Depending on the present concentrations, nickel may favourably, unfavourably or not affect the metabolic processes of plants at all (enzyme activity, photosynthesis, respiration, water regime, mineral nutrition, etc.), and thus the growth and development of plants. There are still many questions about the mechanism of Ni influence on the physiological and biochemical processes of plants. Therefore, we believe the presentation of the previous knowledge about the effect of Ni on plants' life processes can contribute to a better understanding of the biotechnical and ecological significance of Ni, and it can indicate further research directions in this field.
{"title":"Functions of nickel in higher plants: A review","authors":"R. Kastori, M. Putnik-Delić, I. Maksimović","doi":"10.5937/aaser2253089k","DOIUrl":"https://doi.org/10.5937/aaser2253089k","url":null,"abstract":"Nickel (Ni) is an essential microelement for higher plants and an important one for many other living organisms. It is present in every part of the biosphere. Higher plants have small requirements towards Ni for their optimal growth and development. That is why its latent and acute deficiencies in nature rarely occur. Soil and water pollution with Ni is a global issue. This is the reason special attention is paid to the effects of higher Ni concentrations on plants metabolism, their organic production, as well as on the possibility of using plants for phytoremediation of Ni contaminated habitats. Depending on the present concentrations, nickel may favourably, unfavourably or not affect the metabolic processes of plants at all (enzyme activity, photosynthesis, respiration, water regime, mineral nutrition, etc.), and thus the growth and development of plants. There are still many questions about the mechanism of Ni influence on the physiological and biochemical processes of plants. Therefore, we believe the presentation of the previous knowledge about the effect of Ni on plants' life processes can contribute to a better understanding of the biotechnical and ecological significance of Ni, and it can indicate further research directions in this field.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Pešaković, J. Tomić, B. Rilak, Z. Karaklajic-Stajic, L. Mandić, Vesna Đurović, T. Vasić
Anthracnose disease can be successfully controlled by chemical pesticides in conventional fruit growing. However, in organic farming, in order to maintain environmental safety and fulfill consumer demand for pesticide-free food, the control of this disease is a major problem. The use of biocontrol agents with the antagonistic mechanism of control of many phytopathogens is an innovative alternative for cost-effective and eco-friendly production. The antagonistic activities of Trichoderma viride and Bacillus subtilis were tested in vitro against Colletotrichum acutatum, one of major plant pathogens responsible for anthracnose. The microbial antagonists inhibited mycelial growth in the dual culture. T. viride exhibited strong antagonism against C. acutatum isolates (80%). The B. subtilis isolate also had a strong effect on inhibiting the development of C. acutatum (37.5%). The results of this study identified T. viride and B. subtilis as promising biological control agents for further testing against anthracnose disease in fruits.
{"title":"In vitro screening of antagonistic activity of microorganisms against anthracnose disease","authors":"M. Pešaković, J. Tomić, B. Rilak, Z. Karaklajic-Stajic, L. Mandić, Vesna Đurović, T. Vasić","doi":"10.5937/aaser2254165p","DOIUrl":"https://doi.org/10.5937/aaser2254165p","url":null,"abstract":"Anthracnose disease can be successfully controlled by chemical pesticides in conventional fruit growing. However, in organic farming, in order to maintain environmental safety and fulfill consumer demand for pesticide-free food, the control of this disease is a major problem. The use of biocontrol agents with the antagonistic mechanism of control of many phytopathogens is an innovative alternative for cost-effective and eco-friendly production. The antagonistic activities of Trichoderma viride and Bacillus subtilis were tested in vitro against Colletotrichum acutatum, one of major plant pathogens responsible for anthracnose. The microbial antagonists inhibited mycelial growth in the dual culture. T. viride exhibited strong antagonism against C. acutatum isolates (80%). The B. subtilis isolate also had a strong effect on inhibiting the development of C. acutatum (37.5%). The results of this study identified T. viride and B. subtilis as promising biological control agents for further testing against anthracnose disease in fruits.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to compare the yield and fruit quality characteristics of raspberry (Rubus idaeus L.) cultivar ʽMeeker' grown in organic and conventional cultivation systems. The total soluble solids, titratable acidity, ascorbic acid content, total phenolic and flavonoid contents and total antioxidant capacity of raspberry fruits were assessed. The analyses showed that raspberry yields per cane were significantly higher in the conventional cultivation system. In contrast, fruits from organic farming had higher antioxidant levels. The findings of this study indicated that organic farming in the study region increased raspberry quality, but decreased yield, as compared to conventional farming.
{"title":"Yield and fruit quality of 'Meeker' raspberry from conventional and organic cultivation systems","authors":"S. Murtić, Jasmin Fazlić, Amina Šerbo, Mirza Valjevac, Imran Muharemović, Fahrudin Topčić","doi":"10.5937/aaser2254143m","DOIUrl":"https://doi.org/10.5937/aaser2254143m","url":null,"abstract":"The aim of this study was to compare the yield and fruit quality characteristics of raspberry (Rubus idaeus L.) cultivar ʽMeeker' grown in organic and conventional cultivation systems. The total soluble solids, titratable acidity, ascorbic acid content, total phenolic and flavonoid contents and total antioxidant capacity of raspberry fruits were assessed. The analyses showed that raspberry yields per cane were significantly higher in the conventional cultivation system. In contrast, fruits from organic farming had higher antioxidant levels. The findings of this study indicated that organic farming in the study region increased raspberry quality, but decreased yield, as compared to conventional farming.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study was to analyze the use of avocado pulp in place of egg yolk in the preparation of a mayonnaise-like paste. Eight formulations of mayonnaise were obtained with avocado pulp ranging from 51.2 to 70% and sunflower oil ranging from 30 to 48.68%, with all other constituents remaining constant. Sensory evaluation revealed that the mayonnaise made from 55% avocado pulp and 45% sunflower oil was the best when compared to marketed products in terms of sensory evaluation, proximate analysis, microbiological analysis, acid value, and peroxide value. The optimized product was superior in terms of color and overall acceptability than the control sample (P < 0.05). The chemical analysis, i.e., the moisture, fat, protein, ash and carbohydrate contents were found to be 40.54%, 51.1%, 1.22%, 1.71% and 6.23%, respectively, for the optimized product and 26.41%, 66.22%, 0.34%, 1.11% and 6.33%, respectively, for the control sample. Total plate count and yeast and mold count (cfu/g) were found to be 170.3 and 89.33, respectively, for the optimized product and 160.67 and 84, respectively, for the control product. There was an increase in both acid value and peroxide value at all temperatures during storage but a lower increase was found in the sample stored at 25°C. Using the accelerated shelf life technique, the shelf life of optimized mayonnaise like paste based on acid value was found to be 61 days, 41 days and 13 days at 25°C, 40°C and 50°C, respectively. Similarly, the shelf life based on peroxide value was found to be 35 days, 20 days and 12 days at 25°C, 40°C and 50 °C, respectively. Avocado incorporated mayonnaise with acceptable sensory quality and good spreadability can be prepared. Determinations of acid and peroxide values were carried out at regular intervals, ranging from 5 days to 30 days during the storage of mayonnaise. No chemical analysis was done for longer than 30 days, and also no microbiological analysis was performed for the entire storage period; these analyses would be necessary in further research to confirm the shelf life of mayonnaise.
{"title":"Optimization and shelf-life evaluation of mayonnaise like paste prepared using avocado pulp","authors":"A. Parajuli, Nirat Katuwal, A. Dangal","doi":"10.5937/aaser2254149p","DOIUrl":"https://doi.org/10.5937/aaser2254149p","url":null,"abstract":"The purpose of this study was to analyze the use of avocado pulp in place of egg yolk in the preparation of a mayonnaise-like paste. Eight formulations of mayonnaise were obtained with avocado pulp ranging from 51.2 to 70% and sunflower oil ranging from 30 to 48.68%, with all other constituents remaining constant. Sensory evaluation revealed that the mayonnaise made from 55% avocado pulp and 45% sunflower oil was the best when compared to marketed products in terms of sensory evaluation, proximate analysis, microbiological analysis, acid value, and peroxide value. The optimized product was superior in terms of color and overall acceptability than the control sample (P < 0.05). The chemical analysis, i.e., the moisture, fat, protein, ash and carbohydrate contents were found to be 40.54%, 51.1%, 1.22%, 1.71% and 6.23%, respectively, for the optimized product and 26.41%, 66.22%, 0.34%, 1.11% and 6.33%, respectively, for the control sample. Total plate count and yeast and mold count (cfu/g) were found to be 170.3 and 89.33, respectively, for the optimized product and 160.67 and 84, respectively, for the control product. There was an increase in both acid value and peroxide value at all temperatures during storage but a lower increase was found in the sample stored at 25°C. Using the accelerated shelf life technique, the shelf life of optimized mayonnaise like paste based on acid value was found to be 61 days, 41 days and 13 days at 25°C, 40°C and 50°C, respectively. Similarly, the shelf life based on peroxide value was found to be 35 days, 20 days and 12 days at 25°C, 40°C and 50 °C, respectively. Avocado incorporated mayonnaise with acceptable sensory quality and good spreadability can be prepared. Determinations of acid and peroxide values were carried out at regular intervals, ranging from 5 days to 30 days during the storage of mayonnaise. No chemical analysis was done for longer than 30 days, and also no microbiological analysis was performed for the entire storage period; these analyses would be necessary in further research to confirm the shelf life of mayonnaise.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Nikolić, M. Simić, S. Žilić, N. Kravić, Vojka Babić, M. Filipović, J. Srdić
This study's primary objective was to assess the quality parameters of fifteen different genotypes of Serbian maize hybrids in order to establish whether they were suitable for making high-quality silage for ruminant feed. The study was carried out as a two-year field experiment at the Maize Research Institute in Zemun Polje, Serbia. Laboratory analyses included the yield structure of the examined maize hybrids, the evaluation of the composition of lignocellulosic fiber and the percentage of their ratios, as well as the in vitro dry matter digestibility and NDF digestibility of the whole plant samples. The Fisher's LSD test was used to statistically analyse the data, which were then reported as the mean and standard deviation of at least three independent replicates. The graphical representation of correlations among the observed parameters used the principal component analysis (PCA) method. All maize hybrids have displayed high-quality characteristics, which are required for the production of high-quality silage.
{"title":"Quality parameters of maize hybrids intended for silage production","authors":"V. Nikolić, M. Simić, S. Žilić, N. Kravić, Vojka Babić, M. Filipović, J. Srdić","doi":"10.5937/aaser2254157n","DOIUrl":"https://doi.org/10.5937/aaser2254157n","url":null,"abstract":"This study's primary objective was to assess the quality parameters of fifteen different genotypes of Serbian maize hybrids in order to establish whether they were suitable for making high-quality silage for ruminant feed. The study was carried out as a two-year field experiment at the Maize Research Institute in Zemun Polje, Serbia. Laboratory analyses included the yield structure of the examined maize hybrids, the evaluation of the composition of lignocellulosic fiber and the percentage of their ratios, as well as the in vitro dry matter digestibility and NDF digestibility of the whole plant samples. The Fisher's LSD test was used to statistically analyse the data, which were then reported as the mean and standard deviation of at least three independent replicates. The graphical representation of correlations among the observed parameters used the principal component analysis (PCA) method. All maize hybrids have displayed high-quality characteristics, which are required for the production of high-quality silage.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Vuković, V. Bursić, T. Stojanović, N. Puvača, D. Marinković, A. Petrovič, B. Konstantinović, N. Samardžić, M. Popov
Alternaria mycotoxins are the secondary metabolites for which the regulations, as well as the standardization, are yet going to be established by the European Commission and the European Committee for Standardization. This paper describes the possibility to apply a "dilute-and-shoot" method for the determination of tentoxin, alternariol and alternariol monomethyl ether in wheat. The used chromatographic method was liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The strategy involves extraction the samples with the acetonitrile and water mixture (84/16, v/v), followed by the vortexing, centrifugation and filtration before the injection into the LC-MS/MS system. The recovery was assessed by analysis of spiked samples with a mixture of standard solutions of all three mycotoxins at two spiking levels (0.02 and 0.1 mg kg-1) in six replicates. The obtained average recoveries and precisions (expressed as the RSDr, %) for "dilute-and-shoot" method were 76.3% (RSDr of 6.47%) for alternariol, 105.2% (RSDr of 2.16%) for tentoxin, and 86.0% (RSDr of 3.91%) for alternariol monomethyl ether. All the obtained validation data were in accordance with the Commission Decision 2002/657/EC and Commission Regulation (EC) No 401/2006. The main advantages of the present method are the simple and easy sample preparation, along with the high-sensitivity detection.
交替菌毒素是次级代谢物，欧盟委员会和欧洲标准化委员会尚未对其制定法规和标准化。本文介绍了用“稀释-射击”法测定小麦中藤毒素、互胺醇和互胺醇单甲醚含量的可能性。色谱方法为液相色谱-串联质谱联用(LC-MS/MS)。该策略包括用乙腈和水的混合物(84/16,v/v)提取样品，然后进行涡流、离心和过滤，然后进入LC-MS/MS系统。在6个重复中，用所有三种真菌毒素的标准溶液的混合物对加标样品进行两种加标水平(0.02和0.1 mg kg-1)的分析，以评估回收率。“稀释射击法”的平均加样回收率和精密度(RSDr， %)分别为:交替蒿油76.3% (RSDr为6.47%)、藤毒素105.2% (RSDr为2.16%)、交替蒿油单甲醚86.0% (RSDr为3.91%)。所有获得的验证数据均符合欧盟委员会决议2002/657/EC和欧盟委员会法规(EC) No 401/2006。该方法的主要优点是样品制备简单，检测灵敏度高。
{"title":"A \"dilute-and-shoot\" method for the Alternaria mycotoxins determination in wheat","authors":"G. Vuković, V. Bursić, T. Stojanović, N. Puvača, D. Marinković, A. Petrovič, B. Konstantinović, N. Samardžić, M. Popov","doi":"10.5937/aaser2253073v","DOIUrl":"https://doi.org/10.5937/aaser2253073v","url":null,"abstract":"Alternaria mycotoxins are the secondary metabolites for which the regulations, as well as the standardization, are yet going to be established by the European Commission and the European Committee for Standardization. This paper describes the possibility to apply a \"dilute-and-shoot\" method for the determination of tentoxin, alternariol and alternariol monomethyl ether in wheat. The used chromatographic method was liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The strategy involves extraction the samples with the acetonitrile and water mixture (84/16, v/v), followed by the vortexing, centrifugation and filtration before the injection into the LC-MS/MS system. The recovery was assessed by analysis of spiked samples with a mixture of standard solutions of all three mycotoxins at two spiking levels (0.02 and 0.1 mg kg-1) in six replicates. The obtained average recoveries and precisions (expressed as the RSDr, %) for \"dilute-and-shoot\" method were 76.3% (RSDr of 6.47%) for alternariol, 105.2% (RSDr of 2.16%) for tentoxin, and 86.0% (RSDr of 3.91%) for alternariol monomethyl ether. All the obtained validation data were in accordance with the Commission Decision 2002/657/EC and Commission Regulation (EC) No 401/2006. The main advantages of the present method are the simple and easy sample preparation, along with the high-sensitivity detection.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71194171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tamer Tashla, M. Ćosić, V. Kurćubić, R. Prodanović, N. Puvača
The objective of this paper was to evaluate oxidative stress during different pregnancy stages in Lohi sheep reared in Libya. Investigations were carried out on Lohi sheep between 1.5 and 5 years of age. A total of 40 sheep were sub-grouped into various stages of pregnancy along with non-pregnant controls as follows: non-pregnant (n=10), early pregnancy (n=10), mid pregnancy (n=10), and late pregnancy (n=10). Blood samples were collected from pregnant sheep and preserved at -20°C and further used for evaluating the concentrations of total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and paraoxonase (PON-1). In pregnant sheep, TAS, TOS, MDA, SOD, CAT, and PON-1 values were significantly (P < 0.05) different. TOS and MDA increased from non-pregnant ewes to early, mid and late stages of pregnancy whereas TAS, SOD, CAT, and PON-1 significantly (P < 0.05) decreased with the progression of pregnancy. The study suggests that the use of oxidant and antioxidant markers along with the blood metabolic profile should be the recommended procedure for monitoring health status during pregnancy to evade reduced productive performance and economic losses.
{"title":"Occurrence of oxidative stress in sheep during different pregnancy periods","authors":"Tamer Tashla, M. Ćosić, V. Kurćubić, R. Prodanović, N. Puvača","doi":"10.5937/aaser2152111t","DOIUrl":"https://doi.org/10.5937/aaser2152111t","url":null,"abstract":"The objective of this paper was to evaluate oxidative stress during different pregnancy stages in Lohi sheep reared in Libya. Investigations were carried out on Lohi sheep between 1.5 and 5 years of age. A total of 40 sheep were sub-grouped into various stages of pregnancy along with non-pregnant controls as follows: non-pregnant (n=10), early pregnancy (n=10), mid pregnancy (n=10), and late pregnancy (n=10). Blood samples were collected from pregnant sheep and preserved at -20°C and further used for evaluating the concentrations of total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and paraoxonase (PON-1). In pregnant sheep, TAS, TOS, MDA, SOD, CAT, and PON-1 values were significantly (P < 0.05) different. TOS and MDA increased from non-pregnant ewes to early, mid and late stages of pregnancy whereas TAS, SOD, CAT, and PON-1 significantly (P < 0.05) decreased with the progression of pregnancy. The study suggests that the use of oxidant and antioxidant markers along with the blood metabolic profile should be the recommended procedure for monitoring health status during pregnancy to evade reduced productive performance and economic losses.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Marić, I. Glišić, N. Milosevic, S. Radičević, M. Đorđević, T. Vujović
Ethylene is the simplest signalling molecule with a hormone-like function that plays a major role in many developmental processes, including ripening of climacteric apple fruit. The allelic polymorphisms of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase, which catalyse the last two steps in the ethylene biochemical pathway, were analysed in nineteen indigenous apple accessions grown in individual growers' orchards in the regions of central and southwestern Serbia. A polymorphism was detected using the polymerase chain reaction (PCR) for the ACS1 gene and the additional enzymatic digestion of the PCR product with BamH1 and RsaI for the ACO1 gene. The ACS1-1 and ACS1-2 alleles of the ACS1 gene, as well as the a and c alleles of the ACO1 gene were identified. The polymorphisms observed upon PCRs and digestion with restriction enzymes were generated in two genotypes for both genes, i.e. the ACS1 gene - ACS1-1/1 and ACS1-1/2, and the ACO1 gene - aa and ac. Out of nineteen apple accessions, sixteen were homozygous for the allele ACS1-1 and three were heterozygous (ACS1-1/2); regarding the ACO1 genotype, fourteen were homozygous for the allele a and five were heterozygous (ac allelic constitution). The molecular survey in the current study provides an increase in the number of apple accessions with potential to be used as parents in breeding programmes, aiming to obtain high quality cultivars that retain fruit texture during long storage. Therefore, the heterozygous accessions for the ACS1 gene - 'J-LuN/1', 'Kraljica' and 'Šumatovka', may have an important position in future breeding programmes.
{"title":"Characterisation of indigenous apple accessions with respect to polymorphism of ACS1 and ACO1 genes","authors":"S. Marić, I. Glišić, N. Milosevic, S. Radičević, M. Đorđević, T. Vujović","doi":"10.5937/aaser2152151m","DOIUrl":"https://doi.org/10.5937/aaser2152151m","url":null,"abstract":"Ethylene is the simplest signalling molecule with a hormone-like function that plays a major role in many developmental processes, including ripening of climacteric apple fruit. The allelic polymorphisms of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase, which catalyse the last two steps in the ethylene biochemical pathway, were analysed in nineteen indigenous apple accessions grown in individual growers' orchards in the regions of central and southwestern Serbia. A polymorphism was detected using the polymerase chain reaction (PCR) for the ACS1 gene and the additional enzymatic digestion of the PCR product with BamH1 and RsaI for the ACO1 gene. The ACS1-1 and ACS1-2 alleles of the ACS1 gene, as well as the a and c alleles of the ACO1 gene were identified. The polymorphisms observed upon PCRs and digestion with restriction enzymes were generated in two genotypes for both genes, i.e. the ACS1 gene - ACS1-1/1 and ACS1-1/2, and the ACO1 gene - aa and ac. Out of nineteen apple accessions, sixteen were homozygous for the allele ACS1-1 and three were heterozygous (ACS1-1/2); regarding the ACO1 genotype, fourteen were homozygous for the allele a and five were heterozygous (ac allelic constitution). The molecular survey in the current study provides an increase in the number of apple accessions with potential to be used as parents in breeding programmes, aiming to obtain high quality cultivars that retain fruit texture during long storage. Therefore, the heterozygous accessions for the ACS1 gene - 'J-LuN/1', 'Kraljica' and 'Šumatovka', may have an important position in future breeding programmes.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wild or dog rose (Rosa canina L.) is a successful colonizer of various habitats and different soil types and is widely distributed across the Republic of Croatia. In this research, in order to estimate pomological variability in native dog rose populations, four genotypes from four locations in different geographic areas of Croatia were selected and sampled. The genotypes selected were: genotype G1, originating from the continental part of Croatia (Pitomača); genotypes G2 and G3, originating from the Mediterranean part of Croatia (Kukurini and Posedarje, respectively); and genotype G4, originating from the upland part of Croatia (Gračac). Fruits were harvested at optimum harvest dates in 2010 and 2012. Genotype had a significant effect on each studied pomological trait (length, width, geometric mean diameter, sphericity, volume, surface, shape index, weight, flesh weight, flesh ratio and total dry matter content), while year significantly affected all parameters except sphericity and shape index. The highest values for most pomological traits in 2010 and 2012 were found in the G4 and G3 genotypes, respectively. This research highlighted the existence of high variability in pomological traits among dog rose populations in Croatia, which emphasizes the possibility of further breeding and cultivation.
{"title":"Variability in pomological traits of dog rose (Rosa canina L.) under the ecological conditions of the Republic of Croatia","authors":"Nikola Tomljenović, T. Jemrić, M. Vuković","doi":"10.5937/aaser2151041t","DOIUrl":"https://doi.org/10.5937/aaser2151041t","url":null,"abstract":"Wild or dog rose (Rosa canina L.) is a successful colonizer of various habitats and different soil types and is widely distributed across the Republic of Croatia. In this research, in order to estimate pomological variability in native dog rose populations, four genotypes from four locations in different geographic areas of Croatia were selected and sampled. The genotypes selected were: genotype G1, originating from the continental part of Croatia (Pitomača); genotypes G2 and G3, originating from the Mediterranean part of Croatia (Kukurini and Posedarje, respectively); and genotype G4, originating from the upland part of Croatia (Gračac). Fruits were harvested at optimum harvest dates in 2010 and 2012. Genotype had a significant effect on each studied pomological trait (length, width, geometric mean diameter, sphericity, volume, surface, shape index, weight, flesh weight, flesh ratio and total dry matter content), while year significantly affected all parameters except sphericity and shape index. The highest values for most pomological traits in 2010 and 2012 were found in the G4 and G3 genotypes, respectively. This research highlighted the existence of high variability in pomological traits among dog rose populations in Croatia, which emphasizes the possibility of further breeding and cultivation.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Mamlic, I. Maksimović, J. Crnobarac, V. Đorđević, Marina Delić-Putnik, V. Đukić, A. Uhlarik
Priming of legume seeds before sowing was performed by Roman farmers in order to increase the germination rate and synchronize germination, as reported by the Roman naturalist Gaius Plinius Secundus. Several centuries later, this technique is still used for a wide range of species. However, in order for this measure to be used successfully in production, it is necessary to find a solution for the successful storage of primed seeds. The storage potential of primed soybean seeds was determined by a temperature of 25 °C during the period of 90 days. Soybean seeds were primed with KNO3 (1%), AsA (100 mg L-1) and KCl (1%) solutions, and then stored in natron paper bags, and their quality was tested every 15 days. The results showed that a reduction in the quality of primed seeds was considerably faster than in non-primed seeds. Primed soybean seeds were successfully stored at a temperature of 25 °C for 60 days after priming, and then a significant reduction in their quality occurred. One of the causes of seed quality reduction was an increase in MDA content, especially after 75 and 90 days of storage. Also, free proline concentration was reduced while the content of vitamin C increased after 15 days primarily in seeds primed in AsA and KCl solutions, and decreased after 45 days.
{"title":"How long can primed soybean (Glycine max L.) seeds be stored in natron paper bags?","authors":"Z. Mamlic, I. Maksimović, J. Crnobarac, V. Đorđević, Marina Delić-Putnik, V. Đukić, A. Uhlarik","doi":"10.5937/aaser2152145m","DOIUrl":"https://doi.org/10.5937/aaser2152145m","url":null,"abstract":"Priming of legume seeds before sowing was performed by Roman farmers in order to increase the germination rate and synchronize germination, as reported by the Roman naturalist Gaius Plinius Secundus. Several centuries later, this technique is still used for a wide range of species. However, in order for this measure to be used successfully in production, it is necessary to find a solution for the successful storage of primed seeds. The storage potential of primed soybean seeds was determined by a temperature of 25 °C during the period of 90 days. Soybean seeds were primed with KNO3 (1%), AsA (100 mg L-1) and KCl (1%) solutions, and then stored in natron paper bags, and their quality was tested every 15 days. The results showed that a reduction in the quality of primed seeds was considerably faster than in non-primed seeds. Primed soybean seeds were successfully stored at a temperature of 25 °C for 60 days after priming, and then a significant reduction in their quality occurred. One of the causes of seed quality reduction was an increase in MDA content, especially after 75 and 90 days of storage. Also, free proline concentration was reduced while the content of vitamin C increased after 15 days primarily in seeds primed in AsA and KCl solutions, and decreased after 45 days.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：