Two naturally occurring fungal and bacterial species, Aspergillus flavus and Bacillus sp. H6 strains, were capable of utilizing diesel oil as a sole source of carbon in synthetic microcosoms. The initial diesel oil contamination of 1666 mg kg dry soil was reduced to 166.667 mg kg after 150 days of incubation in fungal-bacterial consortium microcosm. That is mean 89.9%, of the initial oil concentration was removed. Abiotic process reduced the diesel oil contamination to about 616 mg kgdry soil at the end of the experiment. Seven microcosms were set up to fulfill the experiments. The decontamination activity follow this order; Bacillus sp. H6 + A. flavus consortium > Bacillus sp. H6 > natural control > A. flavus > cycloheximide treated > benzyl Penicillin-Streptomycin treated > poisoned control. Gas chromatographic analysis data revealed that both A. flavus and Bacillus sp. H6 treatment led to complete utilization of carbon-17 compounds. Other biodegradation products such as C-15, 16, 20, 21, and 24 appears in the chromatogram after 150 days incubation. Increase of C-20, 21 and C-24 compounds also noticed. The fungaland bacterium consortium treatment depicted a decrease of all detected n-alkanes. The microbial success in biodegradation was evaluated by determining the number of germinating seeds of Phaseolus vulgaris. The highest level of germination (92 %) was detected in consortium microcosms after 150 days incubation. The treatment with A. flavus and Bacillus sp. H6 separately led to a lower percentage of germination (86%). The other treatments showed variable results except the poisoned control that showed negative germination and minor chemical degradation of diesel oil. Thus, bacterial-fungal consortium treatment is effective in bioremediation of contaminated oils than separate treatment.
{"title":"Assessment of diesel degrading potential of fungal and bacterial isolates from Egypt","authors":"MT Mohesien, E. El-Morsy, M. Abou-Dobara","doi":"10.21608/mb.2018.26272","DOIUrl":"https://doi.org/10.21608/mb.2018.26272","url":null,"abstract":"Two naturally occurring fungal and bacterial species, Aspergillus flavus and Bacillus sp. H6 strains, were capable of utilizing diesel oil as a sole source of carbon in synthetic microcosoms. The initial diesel oil contamination of 1666 mg kg dry soil was reduced to 166.667 mg kg after 150 days of incubation in fungal-bacterial consortium microcosm. That is mean 89.9%, of the initial oil concentration was removed. Abiotic process reduced the diesel oil contamination to about 616 mg kgdry soil at the end of the experiment. Seven microcosms were set up to fulfill the experiments. The decontamination activity follow this order; Bacillus sp. H6 + A. flavus consortium > Bacillus sp. H6 > natural control > A. flavus > cycloheximide treated > benzyl Penicillin-Streptomycin treated > poisoned control. Gas chromatographic analysis data revealed that both A. flavus and Bacillus sp. H6 treatment led to complete utilization of carbon-17 compounds. Other biodegradation products such as C-15, 16, 20, 21, and 24 appears in the chromatogram after 150 days incubation. Increase of C-20, 21 and C-24 compounds also noticed. The fungaland bacterium consortium treatment depicted a decrease of all detected n-alkanes. The microbial success in biodegradation was evaluated by determining the number of germinating seeds of Phaseolus vulgaris. The highest level of germination (92 %) was detected in consortium microcosms after 150 days incubation. The treatment with A. flavus and Bacillus sp. H6 separately led to a lower percentage of germination (86%). The other treatments showed variable results except the poisoned control that showed negative germination and minor chemical degradation of diesel oil. Thus, bacterial-fungal consortium treatment is effective in bioremediation of contaminated oils than separate treatment.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"253 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79443071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-08DOI: 10.21608/mb.2018.4276.1001
A. Darwish, M. Allam, E. Ayad
{"title":"Physicochemical Profile and Lactic Acid Bacteria Genera Inhabit Egyptian Raw Camel, Sheep, Goat, Buffalo and Cow milks","authors":"A. Darwish, M. Allam, E. Ayad","doi":"10.21608/mb.2018.4276.1001","DOIUrl":"https://doi.org/10.21608/mb.2018.4276.1001","url":null,"abstract":"","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82723091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of dairy-based functional foods has increased markedly over the last fewyears. Ginger is believed to exert a wide range of therapeutic properties. This study aimed toevaluate probiotic properties of twenty Lactic acid bacteria to apply in functional fermented milkfortified with fresh ginger juice to increase the therapeutic and nutritional effects of the product.Ginger juice was prepared from ginger rhizomes through sorting, washing, peeling, crushing andcrude juice extraction. It was assayed for antibacterial activity by minimal inhibitoryconcentration against antibiotic resistant pathogenic bacteria (E. coli BA 12296, Bacillus subtilisDB100, Klebsiella pneumoniaa ATCC12296, Salmonella senftenberg ATCC 8400,Staphylococcus aureus NCTC 10788, Staphylococcus epidermidis ATCC 35984). Total phenol,total flavonoids and antioxidant activity were determined, ginger juice showed an antibacterialactivity and antioxidant activity. Twelve strains out of twenty were resistant to bile saltsconcentrations (0.2, 0.3 and 0.4 w/v), to acid conditions (pH 2.0, 3.0 and 4.0) and were able togrow in the presence of 0.2 and 0.4 w/v of phenol. Seven strains (Lactobacillus delbrueckiisubsp. delbrueckii KT615, Lb. brevis KP653, Lb. delbrueckii subsp. lactis KP645, Lb. plantarumKP623, Lb. paracasei subsp. tolerans WT631, Enterococcus faecalis BM711, Enterococcusfaecium BT734) had the ability to adhere to rabbit intestinal epithelial cells in-vitro, and wereresistant to ginger juice. Ginger juice concentration showed indirect relationship with the milkcoagulation time and direct with syneresis of fermented milk. It was showed that used 2% ofginger juice concentrations was the best results on fermented milk products.
{"title":"Application of some insensitive probiotic lactic acid bacteria and ginger as functional dairy products","authors":"Allam Mg, Gomaa Mae, Ayad Ehe, Darwish Sm","doi":"10.21608/MB.2018.12360","DOIUrl":"https://doi.org/10.21608/MB.2018.12360","url":null,"abstract":"The use of dairy-based functional foods has increased markedly over the last fewyears. Ginger is believed to exert a wide range of therapeutic properties. This study aimed toevaluate probiotic properties of twenty Lactic acid bacteria to apply in functional fermented milkfortified with fresh ginger juice to increase the therapeutic and nutritional effects of the product.Ginger juice was prepared from ginger rhizomes through sorting, washing, peeling, crushing andcrude juice extraction. It was assayed for antibacterial activity by minimal inhibitoryconcentration against antibiotic resistant pathogenic bacteria (E. coli BA 12296, Bacillus subtilisDB100, Klebsiella pneumoniaa ATCC12296, Salmonella senftenberg ATCC 8400,Staphylococcus aureus NCTC 10788, Staphylococcus epidermidis ATCC 35984). Total phenol,total flavonoids and antioxidant activity were determined, ginger juice showed an antibacterialactivity and antioxidant activity. Twelve strains out of twenty were resistant to bile saltsconcentrations (0.2, 0.3 and 0.4 w/v), to acid conditions (pH 2.0, 3.0 and 4.0) and were able togrow in the presence of 0.2 and 0.4 w/v of phenol. Seven strains (Lactobacillus delbrueckiisubsp. delbrueckii KT615, Lb. brevis KP653, Lb. delbrueckii subsp. lactis KP645, Lb. plantarumKP623, Lb. paracasei subsp. tolerans WT631, Enterococcus faecalis BM711, Enterococcusfaecium BT734) had the ability to adhere to rabbit intestinal epithelial cells in-vitro, and wereresistant to ginger juice. Ginger juice concentration showed indirect relationship with the milkcoagulation time and direct with syneresis of fermented milk. It was showed that used 2% ofginger juice concentrations was the best results on fermented milk products.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"1076 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76694044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
d products. This study evaluates functional properties of an ectomycorrhizal wildmushroom Amanita sp. occurring in the lateritic scrub jungles of southwestern India. Based onthe ethnic knowledge, immature cooked fruit bodies of this mushroom are edible. Standardprotocols were followed to evaluate functional properties of uncooked and cooked immaturefruit bodies (pH-dependent protein solubility; least gelation concentration; water- and oilabsorptioncapacities; emulsion and foam properties). The protein solubility was significantlyhigher in uncooked against cooked samples (pH 2-8, p 0.05) despite water-absorption and oil-absorption capacities were higher in cookedsamples. The emulsion activity (p 0.05) were higher in cooked than uncooked samples. The PrincipalComponent Analysis (PCA) between proximal and functional properties reveals that the crudeprotein, total lipids and crude fibre influenced the protein-solubility, emulsion stability and foamcapacity in uncooked samples. In cooked samples, the crude fibre and carbohydrates influencedall the functional properties studied except for protein solubility. It is assumed that thecomposition and proportion of proximal components influence the functional attributes ofAmanita sp. The properties like high emulsion activity, emulsion stability and foam capacity incooked mushroom will be useful in formulation of value-added foods or nutraceutical products.
d产品。本研究评估了发生在印度西南部红土灌丛丛林中的一种外生菌根野生蘑菇Amanita的功能特性。根据民族知识，这种蘑菇未成熟的熟果体是可食用的。按照标准方案评估未熟和未熟果体的功能特性(ph依赖性蛋白质溶解度;最小胶凝浓度;吸水和吸油能力;乳液和泡沫性能)。蛋白质溶解度在未煮熟的样品中明显高于煮熟的样品(pH 2-8, p 0.05)，尽管在煮熟的样品中吸水和吸油能力更高。煮熟的乳化液活性高于未煮熟的乳化液活性(p 0.05)。主成分分析(PCA)表明，粗蛋白、总脂质和粗纤维影响了未煮熟样品的蛋白质溶解度、乳化稳定性和泡沫容量。在煮熟的样品中，粗纤维和碳水化合物影响了除蛋白质溶解度外的所有功能特性。推测近端成分的组成和比例会影响其功能属性，使其具有较高的乳化活性、乳化稳定性和泡沫容量等特性，可用于制作增值食品或营养保健品。
{"title":"Functional attributes of ethnically edible ectomycorrhizal wild mushroom Amanita in India","authors":"Greeshma Aa, Sridhar Kr, M. Pavithra","doi":"10.21608/MB.2018.12358","DOIUrl":"https://doi.org/10.21608/MB.2018.12358","url":null,"abstract":"d products. This study evaluates functional properties of an ectomycorrhizal wildmushroom Amanita sp. occurring in the lateritic scrub jungles of southwestern India. Based onthe ethnic knowledge, immature cooked fruit bodies of this mushroom are edible. Standardprotocols were followed to evaluate functional properties of uncooked and cooked immaturefruit bodies (pH-dependent protein solubility; least gelation concentration; water- and oilabsorptioncapacities; emulsion and foam properties). The protein solubility was significantlyhigher in uncooked against cooked samples (pH 2-8, p 0.05) despite water-absorption and oil-absorption capacities were higher in cookedsamples. The emulsion activity (p 0.05) were higher in cooked than uncooked samples. The PrincipalComponent Analysis (PCA) between proximal and functional properties reveals that the crudeprotein, total lipids and crude fibre influenced the protein-solubility, emulsion stability and foamcapacity in uncooked samples. In cooked samples, the crude fibre and carbohydrates influencedall the functional properties studied except for protein solubility. It is assumed that thecomposition and proportion of proximal components influence the functional attributes ofAmanita sp. The properties like high emulsion activity, emulsion stability and foam capacity incooked mushroom will be useful in formulation of value-added foods or nutraceutical products.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74087059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Public and private institutions usually possess land space for buildings, play grounds,gardens, avenues and natural vegetation. Such landscapes possess diverse flora, fauna andmicrobiota. Inventory of biodiversity in the vicinity constitutes first step to follow the status ofhabitat based on diversity, carrying capacity and further steps for conservation or rehabilitation.The current study assessed macrofungal diversity in the Yenepoya Campus of southwest India incontinuation of assessment of flora and fauna. This preliminary inventory was carried out in 10habitats (three plantations, bamboo thickets, gardens, acacia groves, avenue trees, lawns, dumpedwood and termite mounds), which yielded 40 species of macrofungi belonging to 31 genera.Among them, twelve species were edible, twelve species were medicinal, two species wereectomycorrhizal and finally one species was entomophagous. Substrates supporting macrofungiinclude soil (particolous), humus (humicolous), woody debris (lignicolous) and insects(entomophagous). Results showed an abundance of five species (Amylosprous campbellii,Daldinia concentrica, Lenzites betulina, Marasmiellus stenophyllus and Schizophyllumcommune), 13 others were common and 22 species were occasional. In addition to flora andfauna, inventory of saprophytic macrofungi (involved in recycling the organic matter) occurringin an institution surroundings help designating the habitat as healthy or regenerated orimpoverished to follow appropriate measures to maintain the status quo, rehabilitation andconservation. This study suggests possibilities of domestication and utilization of several edible,medicinal and ectomycorrhizal fungi in this area.
{"title":"Biodiversity of macrofungi in Yenepoya Campus, Southwest India","authors":"Karun Nc, Bhagya Bs, Sridhar Kr","doi":"10.21608/MB.2018.12354","DOIUrl":"https://doi.org/10.21608/MB.2018.12354","url":null,"abstract":"Public and private institutions usually possess land space for buildings, play grounds,gardens, avenues and natural vegetation. Such landscapes possess diverse flora, fauna andmicrobiota. Inventory of biodiversity in the vicinity constitutes first step to follow the status ofhabitat based on diversity, carrying capacity and further steps for conservation or rehabilitation.The current study assessed macrofungal diversity in the Yenepoya Campus of southwest India incontinuation of assessment of flora and fauna. This preliminary inventory was carried out in 10habitats (three plantations, bamboo thickets, gardens, acacia groves, avenue trees, lawns, dumpedwood and termite mounds), which yielded 40 species of macrofungi belonging to 31 genera.Among them, twelve species were edible, twelve species were medicinal, two species wereectomycorrhizal and finally one species was entomophagous. Substrates supporting macrofungiinclude soil (particolous), humus (humicolous), woody debris (lignicolous) and insects(entomophagous). Results showed an abundance of five species (Amylosprous campbellii,Daldinia concentrica, Lenzites betulina, Marasmiellus stenophyllus and Schizophyllumcommune), 13 others were common and 22 species were occasional. In addition to flora andfauna, inventory of saprophytic macrofungi (involved in recycling the organic matter) occurringin an institution surroundings help designating the habitat as healthy or regenerated orimpoverished to follow appropriate measures to maintain the status quo, rehabilitation andconservation. This study suggests possibilities of domestication and utilization of several edible,medicinal and ectomycorrhizal fungi in this area.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76544325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Informations on Algerian mycobiota are scattered through a wide array of journals,books, dissertations and the lack of comprehensive catalogs or checklists makes difficult toapprehend it diversity. By screening all available bibliographic sources and herbaria catalogues itwas possible to delineate 97 species belonging to 31 genera and from which 9 species have notbeen yet reported in the literature for Algeria. This checklist is the first comprehensive specieslist fully dedicated to Algerian Protozoan Fungal Analogues.
{"title":"Checklist of Algerian fungi – Part 1: Protozoan Fungal Analogues (Myxomycetes)","authors":"S. Amrani, Abdel-Azeem Am","doi":"10.21608/MB.2018.12359","DOIUrl":"https://doi.org/10.21608/MB.2018.12359","url":null,"abstract":"Informations on Algerian mycobiota are scattered through a wide array of journals,books, dissertations and the lack of comprehensive catalogs or checklists makes difficult toapprehend it diversity. By screening all available bibliographic sources and herbaria catalogues itwas possible to delineate 97 species belonging to 31 genera and from which 9 species have notbeen yet reported in the literature for Algeria. This checklist is the first comprehensive specieslist fully dedicated to Algerian Protozoan Fungal Analogues.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86776016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：