Pub Date : 2023-07-24DOI: 10.24880/maeuvfd.1288345
Y. S. Orta, M. Kale, S. Hasırcıoglu, Y. Yıldırım, Ö. Özmen, K. Atlı
A wart lesion was observed near the anus of a female Arabian horse housed in a private equine facility in Bucak District of Burdur Province. During the histopathological examination of the mass, revealed equine sarcoid was revealed. Polymerase chain reaction (PCR) revealed the presence of bovine papillomavirus (BPV) type 10 and type 12 viral genomes in the tissue. It was observed that the combined treatment approach of PAPILEND™® cream + Zylexis™ + AlquermoldTM premix powder was beneficial in treating the sarcoid associated with BPV type 10 and type 12 on the anal region. No recurrence of the lesion was observed during the 6-month follow-up period. In conclusion, it is recommended to diagnose the presence of BPV in horses with warts and to investigate the efficacy of this combined treatment approach in larger populations.
{"title":"A different treatment approach for Bovine papillomavirus in an Arabian horse","authors":"Y. S. Orta, M. Kale, S. Hasırcıoglu, Y. Yıldırım, Ö. Özmen, K. Atlı","doi":"10.24880/maeuvfd.1288345","DOIUrl":"https://doi.org/10.24880/maeuvfd.1288345","url":null,"abstract":"A wart lesion was observed near the anus of a female Arabian horse housed in a private equine facility in Bucak District of Burdur Province. During the histopathological examination of the mass, revealed equine sarcoid was revealed. Polymerase chain reaction (PCR) revealed the presence of bovine papillomavirus (BPV) type 10 and type 12 viral genomes in the tissue. It was observed that the combined treatment approach of PAPILEND™® cream + Zylexis™ + AlquermoldTM premix powder was beneficial in treating the sarcoid associated with BPV type 10 and type 12 on the anal region. No recurrence of the lesion was observed during the 6-month follow-up period. In conclusion, it is recommended to diagnose the presence of BPV in horses with warts and to investigate the efficacy of this combined treatment approach in larger populations.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48033542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-24DOI: 10.24880/maeuvfd.1272564
K. Stamatova-Yovcheva, Ö. Di̇lek, R. Dimitrov, David Yovcvhev
The focus was to investigate the anatomical specifics of v. portae, vv. hepaticae and ductus choledochus by corrosion. We investigated 10 sexually mature, clinically healthy New Zealand White rabbits, 8 months old, weighing 2.8 kg to 3.2 kg. To determine the veins and bile vessels, a cold-curing acrylic-based plastic (Duracryl +) was used. The main portal vessel was an intraorganic continuation of v. portae, after its branching into caudate lobe. The main portal vein was divided into v. portae dextra and v. portae sinistra, when entering lobus hepatis dexter and lobus hepatis sinister. V. portae sinistra caudalis was a branch of v. portae sinistra. The venous drainage of the rabbit liver in was carried out by v. hepatica sinistra caudalis, v. hepatica sinistra, v. hepatica dextra, v. hepatica media and venous vessel in lobus caudatus. V. hepatica sinistra and v. hepatica media had a common origin and took blood from lobus hepatis sinister medialis. V. hepatica dextra drained lobus hepatis dexter. V. hepatica sinistra caudalis was a direct tributary of the caudal vena cava. Ductus hepaticus communis was well developed and collected the bile from the main bile duct. Ductus hepaticus dexter drained lobus hepatis dexter and evacuated the bile into the main bile duct. Ductus hepaticus sinister caudalis flowed directly into ductus hepaticus communis. Ductus hepaticus sinister passed into the main bile duct.
{"title":"Morphological Investigation of the Veins and Bile Vessels of Rabbit Liver","authors":"K. Stamatova-Yovcheva, Ö. Di̇lek, R. Dimitrov, David Yovcvhev","doi":"10.24880/maeuvfd.1272564","DOIUrl":"https://doi.org/10.24880/maeuvfd.1272564","url":null,"abstract":"The focus was to investigate the anatomical specifics of v. portae, vv. hepaticae and ductus choledochus by corrosion. We investigated 10 sexually mature, clinically healthy New Zealand White rabbits, 8 months old, weighing 2.8 kg to 3.2 kg. To determine the veins and bile vessels, a cold-curing acrylic-based plastic (Duracryl +) was used. The main portal vessel was an intraorganic continuation of v. portae, after its branching into caudate lobe. The main portal vein was divided into v. portae dextra and v. portae sinistra, when entering lobus hepatis dexter and lobus hepatis sinister. V. portae sinistra caudalis was a branch of v. portae sinistra. The venous drainage of the rabbit liver in was carried out by v. hepatica sinistra caudalis, v. hepatica sinistra, v. hepatica dextra, v. hepatica media and venous vessel in lobus caudatus. V. hepatica sinistra and v. hepatica media had a common origin and took blood from lobus hepatis sinister medialis. V. hepatica dextra drained lobus hepatis dexter. V. hepatica sinistra caudalis was a direct tributary of the caudal vena cava. Ductus hepaticus communis was well developed and collected the bile from the main bile duct. Ductus hepaticus dexter drained lobus hepatis dexter and evacuated the bile into the main bile duct. Ductus hepaticus sinister caudalis flowed directly into ductus hepaticus communis. Ductus hepaticus sinister passed into the main bile duct.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49124898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-23DOI: 10.24880/maeuvfd.1228850
Ertan Doğan
The purpose of this study is to determine the effect of vitamin C on the immune system in cattle immunized against blackleg. The study employed 28 cattle of different breeds and genders, aged 15-20 months, bred in the Ardahan region. The cattle were broken down into four groups, seven in each group. The first group (control group) 2 ml saline, the second group (vitamin group) 5 mg/kg vitamin c, the third group (vaccination group) 2 ml blackleg vaccine, and the fourth group (vaccine-vitamin group) 2 ml Blackleg vaccine and 5 mg/kg vitamin c was administered subcutaneously. Blood samples were collected from all animals in the groups just prior to vaccination and vitamin administration (day zero) and on the 14th and 28th days following administration. The number of formula leukocytes and the amount of serum IgG was measured in the blood samples. Serum IgG was found to increase substantially (p
{"title":"Effect of Vitamin C on the Immune System in Cattle Immunized With Blackleg Vaccine","authors":"Ertan Doğan","doi":"10.24880/maeuvfd.1228850","DOIUrl":"https://doi.org/10.24880/maeuvfd.1228850","url":null,"abstract":"The purpose of this study is to determine the effect of vitamin C on the immune system in cattle immunized against blackleg. The study employed 28 cattle of different breeds and genders, aged 15-20 months, bred in the Ardahan region. The cattle were broken down into four groups, seven in each group. The first group (control group) 2 ml saline, the second group (vitamin group) 5 mg/kg vitamin c, the third group (vaccination group) 2 ml blackleg vaccine, and the fourth group (vaccine-vitamin group) 2 ml Blackleg vaccine and 5 mg/kg vitamin c was administered subcutaneously. Blood samples were collected from all animals in the groups just prior to vaccination and vitamin administration (day zero) and on the 14th and 28th days following administration. The number of formula leukocytes and the amount of serum IgG was measured in the blood samples. Serum IgG was found to increase substantially (p","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-06DOI: 10.24880/maeuvfd.1278378
Nilay Keyvan, Melike Sultan Usluer, M. Kaya, H. Kahraman, H. Tutun, E. Keyvan
Pollen grains, honey, and lactic acid bacteria are combined to make bee bread, which serves as the hive's primary source of nutrition. This study evaluated a Turkish bee bread concerning the total phenolic content, antiradical, and antimicrobial activity against Bacillus cereus, Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella Typhimurium. The Folin-Ciocalteu method was applied to determine the total phenolic content (TPC) in bee bread's aqueous extracts and DPPH was used to assess the extract's radical scavenging activity. The extract's antibacterial activity on foodborne bacteria were evaluated using minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The current study determined the TPC of bee bread to be 24.45 ± 3.75 g of GAE/mg. The DPPH assay results indicated that the water-soluble extract of bee bread (1 mg/mL) had a scavenging activity of 3.40±2.99%. In this study, the bee bread extract's antibacterial effect on S. aureus, E. coli, S. Typhimurium, and B. cereus in various concentrations (6.25 to 25 mg/mL). The bee bread contains phenolic compounds and showed antiradical activity. Also, it has antibacterial activity on all of the tested bacteria. This research contributes to the knowledge of the bioactive properties of this unexplored natural material.
花粉粒、蜂蜜和乳酸菌混合在一起制成蜜蜂面包，这是蜂巢的主要营养来源。本研究评估了土耳其蜜蜂面包的总酚含量、抗自由基和抗蜡样芽孢杆菌、大肠杆菌O157:H7、金黄色葡萄球菌和鼠伤寒沙门氏菌的抗菌活性。采用Folin-Ciocalteu法测定蜜蜂面包水提物中总酚含量(TPC)，并采用DPPH法评价其自由基清除能力。采用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)评价提取物对食源性细菌的抑菌活性。本研究确定蜜蜂面包的TPC为24.45±3.75 g GAE/mg。DPPH测定结果表明，蜜蜂面包水溶性提取物(1 mg/mL)对DPPH的清除活性为3.40±2.99%。本研究考察了不同浓度(6.25 ~ 25 mg/mL)蜜蜂面包提取物对金黄色葡萄球菌、大肠杆菌、鼠伤寒沙门氏菌和蜡样芽孢杆菌的抑菌效果。蜜蜂面包含有酚类化合物，具有抗自由基活性。此外，它对所有被测试的细菌都有抗菌活性。这项研究有助于了解这种未开发的天然材料的生物活性特性。
{"title":"Total Phenolic Content, Antibacterial and Antiradical Properties of Bee Bread from Turkey","authors":"Nilay Keyvan, Melike Sultan Usluer, M. Kaya, H. Kahraman, H. Tutun, E. Keyvan","doi":"10.24880/maeuvfd.1278378","DOIUrl":"https://doi.org/10.24880/maeuvfd.1278378","url":null,"abstract":"Pollen grains, honey, and lactic acid bacteria are combined to make bee bread, which serves as the hive's primary source of nutrition. This study evaluated a Turkish bee bread concerning the total phenolic content, antiradical, and antimicrobial activity against Bacillus cereus, Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella Typhimurium. The Folin-Ciocalteu method was applied to determine the total phenolic content (TPC) in bee bread's aqueous extracts and DPPH was used to assess the extract's radical scavenging activity. The extract's antibacterial activity on foodborne bacteria were evaluated using minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The current study determined the TPC of bee bread to be 24.45 ± 3.75 g of GAE/mg. The DPPH assay results indicated that the water-soluble extract of bee bread (1 mg/mL) had a scavenging activity of 3.40±2.99%. In this study, the bee bread extract's antibacterial effect on S. aureus, E. coli, S. Typhimurium, and B. cereus in various concentrations (6.25 to 25 mg/mL). The bee bread contains phenolic compounds and showed antiradical activity. Also, it has antibacterial activity on all of the tested bacteria. This research contributes to the knowledge of the bioactive properties of this unexplored natural material.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-21DOI: 10.24880/maeuvfd.1226362
Evrim Sönmez, Arzu GÜRSOY ERGEN, S. Çenesiz, Ayris Gökçeoğlu, M. Öğün, E. Uzlu, M. Çenesi̇z
Antioxidants provide protection against free radicals formed as a result of increased metabolism in living organisms and the damage these radicals cause to the cell. For this reason, determining the antioxidant levels can help eco-physiologists in the field in understanding the physiological state of the animal at that moment and in conservation biology. In this study, TAC, TOC and OSI values of 12 Long-legged Buzzards (Buteo rufinus), 7 Common Buzzards (Buteo buteo) and 6 Golden Eagles (Aquila chrysaetos), 15 Grey Herons (Ardea cinerea), 7 Eurasian Eagle Owls (Bubo bubo) brought to rehabilitation centre with injuries due to various reasons were examined. First of all, physical examinations of the birds brought to the centre were made. They were then kept in species-specific rooms until recovery. After they were rehabilitated, blood was taken from the birds 1-2 days before they were released into the nature. As a result of blood analysis, TOC and OSI values were found to be high in Eurasian Eagle Owl, Golden Eagle, Long-legged Buzzard and Common Buzzards which were brought with a diagnosis of gunshot wounds, soft tissue trauma, femur or wing fractures. Although these birds were rehabilitated, the reason why TOC and OSI values were high in the individuals brought with these diagnoses may be the trauma experienced by these free-living birds and their subsequent detention in captivity. After the birds were treated and rehabilitated, they were released back to nature in habitats specific to each species.
{"title":"Total antioxidant, total oxidant and oxidative stress levels in free-living birds","authors":"Evrim Sönmez, Arzu GÜRSOY ERGEN, S. Çenesiz, Ayris Gökçeoğlu, M. Öğün, E. Uzlu, M. Çenesi̇z","doi":"10.24880/maeuvfd.1226362","DOIUrl":"https://doi.org/10.24880/maeuvfd.1226362","url":null,"abstract":"Antioxidants provide protection against free radicals formed as a result of increased metabolism in living organisms and the damage these radicals cause to the cell. For this reason, determining the antioxidant levels can help eco-physiologists in the field in understanding the physiological state of the animal at that moment and in conservation biology. In this study, TAC, TOC and OSI values of 12 Long-legged Buzzards (Buteo rufinus), 7 Common Buzzards (Buteo buteo) and 6 Golden Eagles (Aquila chrysaetos), 15 Grey Herons (Ardea cinerea), 7 Eurasian Eagle Owls (Bubo bubo) brought to rehabilitation centre with injuries due to various reasons were examined. First of all, physical examinations of the birds brought to the centre were made. They were then kept in species-specific rooms until recovery. After they were rehabilitated, blood was taken from the birds 1-2 days before they were released into the nature. As a result of blood analysis, TOC and OSI values were found to be high in Eurasian Eagle Owl, Golden Eagle, Long-legged Buzzard and Common Buzzards which were brought with a diagnosis of gunshot wounds, soft tissue trauma, femur or wing fractures. Although these birds were rehabilitated, the reason why TOC and OSI values were high in the individuals brought with these diagnoses may be the trauma experienced by these free-living birds and their subsequent detention in captivity. After the birds were treated and rehabilitated, they were released back to nature in habitats specific to each species.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.24880/maeuvfd.1243440
Öner Yildiz, Onur Köse
Ectoparasite infestations are of the most important and neglected diseases of birds. Ectoparasites cause sirious harmful effects such as; irritation, feathers and skin damage, restlessness, anemia, weight loss, transmission of other pathogens, reducing overall fitness and decreasing long term survival depending on the intensity of the infestation. The main objective of this study was to investigate the ectoparasites of budgerigars and canaries in Burdur city of Turkey. The material of the study was consisted of randomly selected 555 budgies in 192 cages and 121 canaries in 49 cages belongs to 12 different breeders in center district of Burdur city, Turkey. The feathers of head, neck, abdomen, legs, tail, beak, under the wings, around the cloaca and feet of all birds, in addition cages and cage equipments have been examined in terms of ectoparasites. As a result; no ectoparasite species were found on/between feathers and other body parts of overall 676 cage birds, however, a large number of Dermanyssus gallinae were found in cages and cage equipments. Dermanyssus gallinae have been detected in rates of 28.65% and 28.57% in budgerigar and canary cages, respectively. The present study is the first investigation study of ectoparasites of budgerigar and canaries in Turkey and rarely in the world. In addition, within this study D. gallinae infestation is being reported for the first time from budgerigar and canaries from Turkey.
{"title":"Investigation of ectoparasites in budgerigar and canaries in Burdur city of Turkey","authors":"Öner Yildiz, Onur Köse","doi":"10.24880/maeuvfd.1243440","DOIUrl":"https://doi.org/10.24880/maeuvfd.1243440","url":null,"abstract":"Ectoparasite infestations are of the most important and neglected diseases of birds. Ectoparasites cause sirious harmful effects such as; irritation, feathers and skin damage, restlessness, anemia, weight loss, transmission of other pathogens, reducing overall fitness and decreasing long term survival depending on the intensity of the infestation. The main objective of this study was to investigate the ectoparasites of budgerigars and canaries in Burdur city of Turkey. The material of the study was consisted of randomly selected 555 budgies in 192 cages and 121 canaries in 49 cages belongs to 12 different breeders in center district of Burdur city, Turkey. The feathers of head, neck, abdomen, legs, tail, beak, under the wings, around the cloaca and feet of all birds, in addition cages and cage equipments have been examined in terms of ectoparasites. As a result; no ectoparasite species were found on/between feathers and other body parts of overall 676 cage birds, however, a large number of Dermanyssus gallinae were found in cages and cage equipments. Dermanyssus gallinae have been detected in rates of 28.65% and 28.57% in budgerigar and canary cages, respectively. The present study is the first investigation study of ectoparasites of budgerigar and canaries in Turkey and rarely in the world. In addition, within this study D. gallinae infestation is being reported for the first time from budgerigar and canaries from Turkey.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48094486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.24880/maeuvfd.1249794
E. Ipek, E. T. Epikmen, Çağatay Nuhay, R. Tunca
In this study, Corynebacterium kutscheri infection was reported in Sprague-Dawley rats that were stressed due to intense exercise during an experimental study. Weight loss, indifference to the environment, fluffy feathers, and hunched posture were observed in affected rats. The lungs contained numerous, randomly distributed, variably sized, slightly raised, cream-colored caseopurulent foci. There were occasional weak adhesions between the lung lobes and the adjacent costal pleura. Multifocal to coalescing necro-suppurative pneumonia with intralesional scattered large colonies of bacteria was observed histopathologically. Adjacent in the pulmonary parenchyma, interalveolar hypercellularity, type II pneumocyte hyperplasia, fibrinonecrotic vasculitis, and pleuritis were observed. Brown-Breen staining revealed gram-positive cocobacilli in the lesion areas. Furthermore, cardiac lesions in which the atria were more severely affected than the ventricles were identified. This lesion was characterized by thickening of the epicardium with intense infiltrates of macrophages admixed with scattered neutrophils. In severely affected rats, this lesion was also involved to the underlying myocardium. Bacterial culture yielded positive growth for C. kutsheri from the lesioned organ. Polymerase chain reaction was used to confirm the presence of genetic material for C. kutscheri. As a result, it was revealed that Sprague-Dawley rats were infected with C. kutscheri due to intense exercise stress. Periodic controls of C. kutscheri have been suggested in units where experimental animals are raised, both because of its negative effects on the results of the studies to be conducted and because of its zoonotic nature.
{"title":"Intense exercise stress may trigger Corynebacterium kutscheri infection in Sprague-Dawley rats","authors":"E. Ipek, E. T. Epikmen, Çağatay Nuhay, R. Tunca","doi":"10.24880/maeuvfd.1249794","DOIUrl":"https://doi.org/10.24880/maeuvfd.1249794","url":null,"abstract":"In this study, Corynebacterium kutscheri infection was reported in Sprague-Dawley rats that were stressed due to intense exercise during an experimental study. Weight loss, indifference to the environment, fluffy feathers, and hunched posture were observed in affected rats. The lungs contained numerous, randomly distributed, variably sized, slightly raised, cream-colored caseopurulent foci. There were occasional weak adhesions between the lung lobes and the adjacent costal pleura. Multifocal to coalescing necro-suppurative pneumonia with intralesional scattered large colonies of bacteria was observed histopathologically. Adjacent in the pulmonary parenchyma, interalveolar hypercellularity, type II pneumocyte hyperplasia, fibrinonecrotic vasculitis, and pleuritis were observed. Brown-Breen staining revealed gram-positive cocobacilli in the lesion areas. Furthermore, cardiac lesions in which the atria were more severely affected than the ventricles were identified. This lesion was characterized by thickening of the epicardium with intense infiltrates of macrophages admixed with scattered neutrophils. In severely affected rats, this lesion was also involved to the underlying myocardium. Bacterial culture yielded positive growth for C. kutsheri from the lesioned organ. Polymerase chain reaction was used to confirm the presence of genetic material for C. kutscheri. As a result, it was revealed that Sprague-Dawley rats were infected with C. kutscheri due to intense exercise stress. Periodic controls of C. kutscheri have been suggested in units where experimental animals are raised, both because of its negative effects on the results of the studies to be conducted and because of its zoonotic nature.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.24880/maeuvfd.1240829
M. Tatar, Kıymet Kübra Tüfekci
The liver is susceptible to toxic effects, as it is the main site of acrylamide biotransformation and detoxification. Researchers have claimed that placental growth factor (PlGF) and its pathway are potentially involved in numerous diseases, including liver fibrosis and angiogenesis. Oleuropein is a natural phenolic compound with potent antioxidant effects. The purpose of this study was to examine the role of PlGF and the potential protection provided by oleuropein in acrylamide hepatotoxicity. Wistar albino rats were assigned into control, acrylamide (ACR) (5 mg/kg), oleuropein (OLE) (4.2 mg/kg), and ACR+OLE groups. Acrylamide and oleuropein were administered for 21 days. The control group received only physiological saline. Liver tissues were evaluated histologically and immunohistochemically. Histological examinations revealed significant enlargement of the sinusoidal vessels and abundant hepatocytes with pyknotic nuclei in the ACR group. Acrylamide toxicity resulted in elevated PlGF, accumulation of 8-hydroxydeoxyguanosine (8-OHdG), and increased Caspase-3 immunoreactivity in the liver. Oleuropein treatment reduced the increased expression of PlGF, 8-OHdG, and Caspase-3 against these deleterious effects observed in the ACR group. A positive correlation was observed between PlGF levels as well as oxidative stress and apoptosis markers in acrylamide toxicity. Oleuropein probably counteracted this mechanism by exhibiting antioxidant activity.
{"title":"Oleuropein attenuates placental growth factor expression by regulating oxidative stress and apoptosis in acrylamide hepatotoxicity","authors":"M. Tatar, Kıymet Kübra Tüfekci","doi":"10.24880/maeuvfd.1240829","DOIUrl":"https://doi.org/10.24880/maeuvfd.1240829","url":null,"abstract":"The liver is susceptible to toxic effects, as it is the main site of acrylamide biotransformation and detoxification. Researchers have claimed that placental growth factor (PlGF) and its pathway are potentially involved in numerous diseases, including liver fibrosis and angiogenesis. Oleuropein is a natural phenolic compound with potent antioxidant effects. The purpose of this study was to examine the role of PlGF and the potential protection provided by oleuropein in acrylamide hepatotoxicity. Wistar albino rats were assigned into control, acrylamide (ACR) (5 mg/kg), oleuropein (OLE) (4.2 mg/kg), and ACR+OLE groups. Acrylamide and oleuropein were administered for 21 days. The control group received only physiological saline. Liver tissues were evaluated histologically and immunohistochemically. Histological examinations revealed significant enlargement of the sinusoidal vessels and abundant hepatocytes with pyknotic nuclei in the ACR group. Acrylamide toxicity resulted in elevated PlGF, accumulation of 8-hydroxydeoxyguanosine (8-OHdG), and increased Caspase-3 immunoreactivity in the liver. Oleuropein treatment reduced the increased expression of PlGF, 8-OHdG, and Caspase-3 against these deleterious effects observed in the ACR group. A positive correlation was observed between PlGF levels as well as oxidative stress and apoptosis markers in acrylamide toxicity. Oleuropein probably counteracted this mechanism by exhibiting antioxidant activity.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69069692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-26DOI: 10.24880/maeuvfd.1230538
B. Sarkarati, Nursel SÖYLEMEZ MİLLİ, O. Eren
The aim of this study is to describe a fast method for the purification of high-purity myoglobin for Mass Spectroscopy analyses and to use it as standard-grade material. A three-step Fast Protein Liquid Chromatography (FPLC) method was used to produce high-purity myoglobin. SEC 650 gel filtration followed by an Enrich Q anion exchange chromatography was used to produce myoglobin in acceptable purity for most research methods. A second filtration step was carried out by narrow field SEC 70 gel to prepare high-purity myoglobin at standard grade purity and capable of Mass Spectroscopy analyses. At least 90% pure myoglobin was obtained by applying two chromatography steps in the samples of three species, and over 99% pure myoglobin was obtained in standard material quality and suitable for mass spectroscopy when the additional narrow field SEC 70 chromatography step was applied. The proposed method provides higher purity compared to other methods and can be applied in a shorter time. FPLC columns significantly reduce the duration of the chromatography steps. At the same time, the use of solid extraction columns instead of dialysis reduced the long overnight dialysis process to a few minutes.
{"title":"A fast protein liquid chromatography method for purification of myoglobin from different species","authors":"B. Sarkarati, Nursel SÖYLEMEZ MİLLİ, O. Eren","doi":"10.24880/maeuvfd.1230538","DOIUrl":"https://doi.org/10.24880/maeuvfd.1230538","url":null,"abstract":"The aim of this study is to describe a fast method for the purification of high-purity myoglobin for Mass Spectroscopy analyses and to use it as standard-grade material. A three-step Fast Protein Liquid Chromatography (FPLC) method was used to produce high-purity myoglobin. SEC 650 gel filtration followed by an Enrich Q anion exchange chromatography was used to produce myoglobin in acceptable purity for most research methods. A second filtration step was carried out by narrow field SEC 70 gel to prepare high-purity myoglobin at standard grade purity and capable of Mass Spectroscopy analyses. At least 90% pure myoglobin was obtained by applying two chromatography steps in the samples of three species, and over 99% pure myoglobin was obtained in standard material quality and suitable for mass spectroscopy when the additional narrow field SEC 70 chromatography step was applied. The proposed method provides higher purity compared to other methods and can be applied in a shorter time. FPLC columns significantly reduce the duration of the chromatography steps. At the same time, the use of solid extraction columns instead of dialysis reduced the long overnight dialysis process to a few minutes.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85843672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.24880/maeuvfd.1172488
S. Okur, L. Yanmaz, Ugur Ersoz, M. Senocak, Ayşe Gölgeli Bedir, F. Turgut, Burak Gümürçi̇nler
Preputioplasty is a surgical procedure performed to increase the orifice diameter and phimosis, which is defined by the inability of the penis to extrude due to the absence or restriction of the preputial opening, is uncommon in cats. A total of eight male cats were referred to Ataturk University Veterinary Faculty Animal Hospital due to the complaint of difficulty in urination and prolonged sitting in the litter box. Narrowed preputial opening and abdominal distension were noted in all cats. The penis was too stenotic to be extruded from the preputial orifice, and urine accumulated in the preputial cavity. The serum biochemistry and hemogram results of cats were within the reference values. After the clinical examination, acquired phimosis was diagnosed. The scar tissues around the preputial orifice that prevented the extrusion of the penis were resected surgically (preputioplasty). Normal urination was recorded following preputioplasty in all cases. In one case, recurrence was postoperatively detected on the 30th day and perineal urethrostomy was performed. Preputioplasty to enlarge the preputial orifice may be used to treat acquired phimosis in cat with a low rate complication.
{"title":"Outcome of preputioplasty in cats with acquired phimosis: Eight cases","authors":"S. Okur, L. Yanmaz, Ugur Ersoz, M. Senocak, Ayşe Gölgeli Bedir, F. Turgut, Burak Gümürçi̇nler","doi":"10.24880/maeuvfd.1172488","DOIUrl":"https://doi.org/10.24880/maeuvfd.1172488","url":null,"abstract":"Preputioplasty is a surgical procedure performed to increase the orifice diameter and phimosis, which is defined by the inability of the penis to extrude due to the absence or restriction of the preputial opening, is uncommon in cats. A total of eight male cats were referred to Ataturk University Veterinary Faculty Animal Hospital due to the complaint of difficulty in urination and prolonged sitting in the litter box. Narrowed preputial opening and abdominal distension were noted in all cats. The penis was too stenotic to be extruded from the preputial orifice, and urine accumulated in the preputial cavity. The serum biochemistry and hemogram results of cats were within the reference values. After the clinical examination, acquired phimosis was diagnosed. The scar tissues around the preputial orifice that prevented the extrusion of the penis were resected surgically (preputioplasty). Normal urination was recorded following preputioplasty in all cases. In one case, recurrence was postoperatively detected on the 30th day and perineal urethrostomy was performed. Preputioplasty to enlarge the preputial orifice may be used to treat acquired phimosis in cat with a low rate complication.","PeriodicalId":32481,"journal":{"name":"Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48198755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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