Insect Biochemistry and Molecular Biology最新文献_第7页

V-ATPase B mediates Bt Cry1Ac binding and toxicity in Grapholita molesta V-ATPase B介导Bt - Cry1Ac的结合和毒性作用。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-24 DOI: 10.1016/j.ibmb.2025.104407

Dandan Pan , Jiayang Feng , Jiacheng Ye, Xiaoyan Yang, Xiaoyan Zhang, Xiaofang Sha, Donghan Wang, Yanshen Fu, Chunxiao Men, Xiangqun Yuan, Yiping Li

Grapholita molesta is a worldwide pest. Cry1Ac is a significant alternative Bacillus thuringiensis (Bt) protein that exhibits substantial toxicity towards Lepidoptera insects. This investigation employed ligand blot, SDS-PAGE and liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques to identify the proteins potentially binding Cry1Ac on the peritrophic membrane (PM) of G. molesta. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed the highest expression of GmolV-ATPase B in the midgut of 4th instar larvae. Following the ingestion of Cry1Ac protoxin by G. molesta larvae, a notable reduction in the expression level of GmolV-ATPase B was observed. The interaction between GmolV-ATPase B and activated Cry1Ac toxin was confirmed through ligand blot and homologous and heterologous competition experiments. Overexpression of GmolV-ATPase B in Sf9 cells led to an increase in Cry1Ac cytotoxicity, while RNAi targeting GmolV-ATPase B resulted in reduced mortality. In vivo bioassays demonstrated that the combined action of GmolV-ATPase B protein and Cry1Ac protoxin significantly enhanced the toxicity of Cry1Ac towards G. molesta larvae compared to Cry1Ac alone. These findings shed light on the binding of Cry1Ac to PM of G. molesta and its insecticidal mechanism, offering a valuable important reference for the development of biopesticides targeting midgut PM proteins.

墨氏笔蝗是一种世界性的害虫。Cry1Ac是一种重要的苏云金芽孢杆菌（Bacillus thuringiensis, Bt）替代蛋白，对鳞翅目昆虫具有显著的毒性。本研究采用配体印迹、SDS-PAGE和液相色谱-串联质谱（LC-MS/MS）技术，鉴定了鼠梨周围营养膜（PM）上可能结合Cry1Ac的蛋白。实时定量聚合酶链反应（RT-qPCR）结果显示，GmolV-ATPase B在4龄幼虫中肠的表达量最高。结果表明，食入Cry1Ac原毒素后，GmolV-ATPase B的表达量显著降低。GmolV-ATPase B与活化的Cry1Ac毒素通过配体印迹和同源、异源竞争实验证实了相互作用。Sf9细胞中过表达GmolV-ATPase B导致Cry1Ac细胞毒性增加，而靶向GmolV-ATPase B的RNAi导致死亡率降低。体内生物实验表明，与单独使用Cry1Ac相比，gmolv - atp酶B蛋白与Cry1Ac原蛋白的联合作用显著增强了Cry1Ac对鼠夜蛾幼虫的毒性。这些研究结果揭示了Cry1Ac与molesta的PM结合及其杀虫机制，为开发靶向中肠PM蛋白的生物农药提供了有价值的重要参考。

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引用次数: 0

Highly efficient hyPBase-mediated transgenesis facilitates gain-of-function analysis in the cricket Gryllus bimaculatus 高效的hypbase介导的转基因促进了蟋蟀双斑鸠的功能获得分析

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-24 DOI: 10.1016/j.ibmb.2025.104405

Takahiro Ohde , Taro Nakamura , Taro Mito , Takaaki Daimon

Gain-of-function analysis is critical to directly assessing gene functions but remains challenging in non-traditional model insects. Recent development of the hyperactive piggyBac transposase (hyPBase) has improved the efficiency of germline transformation in ametabolous and holometabolous insects. Here, we report highly efficient somatic and germline transformations mediated by hyPBase in the cricket Gryllus bimaculatus. hyPBase-mediated misexpression of the melanin pathway gene arylalkylamine N-Acetyltransferase (aaNAT) turned the cricket body color from black to a light color, indicating a broad effect on external phenotype in the injected generation. Mosaic expression of the homeotic gene Antennapedia with hyPBase successfully circumvented the potential embryonic lethality, and induced antenna-to-leg homeotic transformation in a hatched nymph. Our data provide a practical methodology for gain-of-function analysis in the hemimetabolous model to understand key evolutionary innovations in insects.

功能增益分析对于直接评估基因功能至关重要，但在非传统模式昆虫中仍然具有挑战性。近年来，过度活跃的piggyBac转座酶（hyPBase）的发展提高了变性和全变性昆虫的种系转化效率。在这里，我们报道了由hyPBase介导的蟋蟀Gryllus bimaculatus的高效体细胞和种系转化。hypbase介导的黑色素通路基因芳基烷基胺n -乙酰转移酶（aaNAT）的错误表达使蟋蟀的体色从黑色变为浅色，表明在注射代中对外部表型有广泛的影响。同源基因Antennapedia与hyPBase的嵌合表达成功地避免了潜在的胚胎致死性，并在孵化若虫中诱导了天线到腿的同源转化。我们的数据为半代谢模型中的功能获得分析提供了实用的方法，以了解昆虫的关键进化创新。

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引用次数: 0

Cuticular proteins: Essential molecular code for insect survival 表皮蛋白：昆虫生存的基本分子密码

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-22 DOI: 10.1016/j.ibmb.2025.104402

Huitang Qi, Tian Liu

The insect cuticle is essential for survival and environmental adaptation, comprising over 100 cuticular proteins (CPs) that represent approximately 1 % of the insect genome coding genes. This complexity is unique among biological materials, underscoring the diverse roles of CPs in insect biology. However, the reasons for this extensive CP repertoire and their precise functional mechanisms persist as unresolved questions in entomology. Recently, advances in molecular biology and genomics have yielded significant progress in elucidating CP functions. Studies demonstrate that CPs are crucial not only for cuticle formation and development but also for regulating body size, coloration, cuticular structure development, environmental adaptation, immune defense, viral interactions, and insecticide resistance. This review comprehensively summarizes current insect CP research, highlights their diverse physiological and ecological roles, and explores their translational potential for pest management and biomimetic material design.

昆虫角质层对生存和环境适应至关重要，由100多种角质层蛋白（CPs）组成，约占昆虫基因组编码基因的1%。这种复杂性在生物材料中是独一无二的，强调了CPs在昆虫生物学中的不同作用。然而，这种广泛的CP库的原因及其精确的功能机制仍然是昆虫学中未解决的问题。近年来，分子生物学和基因组学的进展使CP功能的研究取得了重大进展。研究表明，CPs不仅对角质层的形成和发育至关重要，而且对调节体型、颜色、角质层结构发育、环境适应、免疫防御、病毒相互作用和杀虫剂抗性也至关重要。本文综述了目前昆虫CP的研究现状，强调了它们在生理和生态方面的多种作用，并探讨了它们在病虫害防治和仿生材料设计方面的应用潜力。

引用次数: 0

Sub-lethal insecticide stress alters epimutation rate but not genetic mutation rate in the pest insect Myzus persicae 亚致死杀虫剂胁迫改变了害虫桃蚜（Myzus persicae）的灭绝率，但没有改变其基因突变率。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-22 DOI: 10.1016/j.ibmb.2025.104403

Benjamin J. Hunt , Kumar Saurabh Singh , Bartlomiej J. Troczka , Joanna Mackisack , Emma Randall , Mark Mallott , Tobias Baril , James Galbraith , Bram Kuijper , Ralf Nauen , Alex Hayward , Chris Bass

Insect pests evolve heritable resistance to insecticides through selection acting on genetic variation generated by mutation. It has been hypothesised that exposure to low doses of pesticides may increase mutation rate in pest genomes and accelerate resistance evolution. However, the impact of pesticide-induced stress on the mutation rate of insects has never been empirically tested. Here we leverage new, high-quality genomic resources for the aphid pest Myzus persicae in conjunction with long-term mutation accumulation experiments to interrogate spontaneous genetic and epigenetic mutation rates in insecticide-exposed and unexposed aphid lines. Our data reveal that multigenerational exposure of Myzus persicae to sublethal concentrations of the neonicotinoid insecticide imidacloprid does not increase genetic mutation rate. Rather we show that imidacloprid exposure results in a significantly lower epimutation rate. These findings reveal the rate of origin and composition of (epi)mutations arising in a pest insect and challenge the proposed link between pesticide exposure and the rate of mutation.

害虫通过选择作用于突变产生的遗传变异，进化出对杀虫剂的遗传抗性。据推测，暴露于低剂量农药可能会增加害虫基因组的突变率并加速抗性进化。然而，农药胁迫对昆虫突变率的影响尚未得到实证检验。在这里，我们利用新的、高质量的蚜虫基因组资源，结合长期的突变积累实验，来调查暴露于杀虫剂和未暴露于杀虫剂的蚜虫系的自发遗传和表观遗传突变率。我们的数据显示，多代接触亚致死浓度的新烟碱类杀虫剂吡虫啉不会增加桃蚜的基因突变率。相反，我们表明，吡虫啉暴露导致显着较低的增殖率。这些发现揭示了害虫中（epi）突变率的起源和组成，并挑战了农药暴露与突变率之间的联系。

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引用次数: 0

miR-927 links nutrient signals and 20-hydroxyecdysone regulation and mediates oviposition in Bactrocera dorsalis miR-927在背小实蝇中连接营养信号和20-羟基脱皮激素调节并介导产卵。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-17 DOI: 10.1016/j.ibmb.2025.104401

Tong-Jun Jin , Zheng-Yang Wang , Qian-Ping Xie , Li-Yuan Zheng , Guy Smagghe , Jin-Jun Wang , Qiang Zhang , Wei Dou

The interplay between lipid homeostasis and hormonal regulation is critical for reproductive success in female insects. The molecular mechanisms linking lipid metabolism to hormonal pathways, particularly ecdysteroid synthesis, remain poorly understood. In this study, we reveal a novel regulatory role of miR-927 in the oriental fruit fly, Bactrocera dorsalis. Using in vivo miRNA mimic/inhibitor assays, combined with an in vitro dual-luciferase reporter and RNA pull-down experiments, we demonstrated that miR-927 directly targets BdCYP315a1, a key enzyme in ecdysteroidogenesis, thereby repressing the 20-hydroxyecdysone (20E) levels. RNA interference of BdCYP315a1 confirmed its essential role in maintaining 20E homeostasis and proper developmental progression. Further, we show that miR-927 expression is regulated by the insulin/insulin-like signaling (IIS) pathway, linking nutritional status to hormonal control. Changes in miR-927, BdCYP315a1, and 20E levels corresponded with alterations in lipid content, ultimately influencing fecundity. These findings reveal a previously uncharacterized miRNA-mediated network integrating nutritional signals, hormonal regulation, and lipid metabolism to control reproductive outcomes. Our work provides novel insights into the molecular mechanisms underlying insect reproduction, highlights miRNAs as key mediators of hormone-lipid interactions, and offers valuable resources for identifying miRNA targets in non-model insect species.

脂质平衡和激素调节之间的相互作用对雌性昆虫的繁殖成功至关重要。脂质代谢与激素途径的分子机制，特别是外甾体合成，仍然知之甚少。在这项研究中，我们揭示了miR-927在东方果蝇小实蝇（Bactrocera dorsalis）中的新调控作用。通过体内miRNA模拟/抑制实验，结合体外双荧光素酶报告基因和RNA下拉实验，我们证明miR-927直接靶向BdCYP315a1，这是脱皮激素形成的关键酶，从而抑制20-羟基脱皮激素（20E）水平。BdCYP315a1的RNA干扰证实了其在维持20E稳态和正常发育进程中的重要作用。此外，我们发现miR-927的表达受胰岛素/胰岛素样信号通路（IIS）的调节，将营养状况与激素控制联系起来。miR-927、BdCYP315a1和20E水平的变化与脂质含量的改变相对应，最终影响生育力。这些发现揭示了一个先前未被描述的mirna介导的网络，整合了营养信号、激素调节和脂质代谢来控制生殖结果。我们的工作为昆虫繁殖的分子机制提供了新的见解，强调了miRNA作为激素-脂质相互作用的关键介质，并为鉴定非模式昆虫物种中的miRNA靶点提供了宝贵的资源。

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引用次数: 0

Is the CncC/Keap1 complex a major factor in conferring pesticide resistance in arthropods? - A critical review CncC/Keap1复合体是节肢动物产生农药抗性的主要因素吗？-批评性评论

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-10 DOI: 10.1016/j.ibmb.2025.104400

Dries Amezian, Thomas Van Leeuwen

The CncC/Keap1 signalling pathway regulates antioxidant and detoxification gene expression in arthropods and is frequently associated with metabolic insecticide resistance. This review critically assesses evidence for its role in resistance phenotypes across key pest species. Although overactivation of CncC/Keap1 correlates with increased detoxification enzyme expression and pesticide tolerance, causal mutations in the coding or regulatory regions of CncC, Keap1, or Maf remain unidentified. We evaluate the evidence supporting the role of CncC/Keap1 in pesticide resistance in insects and mites and report the latest advancements in our understanding of this system in arthropods. We further highlight the need for unbiased genetic mapping and reverse genetic approaches to resolve the mechanisms of constitutive CncC activation in resistant populations. Understanding these mechanisms is crucial for elucidating the origins of metabolic resistance and developing sustainable pest management strategies.

CncC/Keap1信号通路调节节肢动物抗氧化和解毒基因的表达，并经常与代谢性杀虫剂抗性相关。本综述批判性地评估了其在主要害虫物种抗性表型中的作用的证据。尽管CncC/Keap1的过度激活与解毒酶表达增加和农药耐受性相关，但CncC、Keap1或Maf编码或调控区域的因果突变仍未确定。我们评估了支持CncC/Keap1在昆虫和螨的农药抗性中的作用的证据，并报告了我们在节肢动物中对该系统的最新理解进展。我们进一步强调需要无偏遗传作图和反向遗传方法来解决耐药群体中组成型CncC激活的机制。了解这些机制对于阐明代谢抗性的起源和制定可持续的害虫管理策略至关重要。

引用次数: 0

Single-cell transcriptomic profiling reveals diet-dependent dynamics of glucosinolate sulfatases expression and cellular origin in the midgut of Plutella xylostella 单细胞转录组学分析揭示了小菜蛾中肠硫代葡萄糖苷硫酸盐酶表达的饮食依赖动力学和细胞起源。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-09 DOI: 10.1016/j.ibmb.2025.104399

Hanwen Ye , Chanqin Zheng , Bing Wang , Yuqing Zhu , Chenjing Wang , Zhuobing Wang , Zhanjun Lu , Weiyi He , Wei Chen

The diamondback moth (Plutella xylostella), a globally destructive pest, has Brassicaceae as its long-term co-evolved host and can also utilize Fabaceae as an alternative field host. The primary differential factor between these plant families is glucosinolates (GLs). Conventional transcriptome data revealed high midgut expression of glucosinolate sulfatases (GSSs) in response to glucosinolates. However, due to the inability of conventional transcriptomics to resolve cellular heterogeneity, it remained unclear which specific cell types express GSSs and whether exposure to different field hosts triggers distinct differentiation fates in these cells. To address these questions, we constructed a high-resolution atlas of 28,451 midgut cells from larvae reared on radish and pea leaves. Marker gene-based clustering identified 16 distinct cell types, including enterocytes (ECs), enteroendocrine cells (EEs), and intestinal stem cells (ISCs). ScRNA-seq and qRT-PCR analyses revealed host-dependent differential expression patterns of three GSS genes (GSS1-GSS3) among ECs, EEs, and ISCs. Notably, elevated GSS3 protein levels in radish-fed larvae were confirmed by Western blot (WB) and Immunohistochemistry (IHC), while GSS1 and GSS2 exhibited more variable expression patterns. Pseudo-time trajectory analysis further demonstrated that all three GSSs followed differentiation pathways from ISCs into EEs or ECs, but dietary conditions led to distinct differentiation trajectories. This study elucidates the diet-dependent regulatory landscape of GSSs in P. xylostella and delineates the differentiation trajectory of GSS-expressing cells.

小菜蛾（Plutella xylostella）是一种全球性的破坏性害虫，它以十字花科作为长期共同进化的寄主，也可以利用豆科作为替代的田间寄主。这些植物家族之间的主要差异因素是硫代葡萄糖苷（GLs）。常规转录组数据显示，在对硫代葡萄糖苷的反应中，中肠高表达硫代葡萄糖苷硫酸酯酶（gss）。然而，由于传统转录组学无法解决细胞异质性，目前尚不清楚哪些特定细胞类型表达gss，以及暴露于不同的野宿主是否会触发这些细胞的不同分化命运。为了解决这些问题，我们构建了28,451个中肠细胞的高分辨率图谱，这些细胞来自萝卜和豌豆叶上饲养的幼虫。基于标记基因的聚类鉴定出16种不同的细胞类型，包括肠细胞（ECs）、肠内分泌细胞（EEs）和肠干细胞（ISCs）。ScRNA-seq和qRT-PCR分析揭示了三个GSS基因（GSS1-GSS3）在ECs、EEs和ISCs中宿主依赖性的差异表达模式。Western blot （WB）和免疫组化（IHC）结果显示，萝卜饲幼虫中GSS3蛋白水平升高，而GSS1和GSS2表达模式变化较大。伪时间轨迹分析进一步表明，所有三种gss都遵循从ISCs到EEs或ECs的分化途径，但饮食条件导致不同的分化轨迹。本研究阐明了小菜中gss的饮食依赖性调控格局，并描绘了gss表达细胞的分化轨迹。

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引用次数: 0

Horizontally transferred NADAR genes contribute to immune defense of ladybird beetles against bacterial infection 水平转移的NADAR基因有助于瓢虫抵抗细菌感染的免疫防御

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-08 DOI: 10.1016/j.ibmb.2025.104397

Kun-Yu Yang, Yi-Fei Sun, Yuan-Sen Liang, Hao Li, Meng-Xue Qi, Zhaowei Wang, Luis Carlos Ramos Aguila, Li-Qun Cai, Hao-Sen Li, Hong Pang

Horizontal gene transfer (HGT) is now widely recognized as an important mechanism contributing to host immunity and adaptation. Ladybird beetles, with their diverse diets and habitats, encounter a broad spectrum of microbial threats, making effective immune responses critical for their survival. However, the immune roles of HGT-acquired genes in ladybirds remain largely unexplored. To address this gap, we investigated HGT of a NADAR (NAD- and ADP-ribose-associated) domain-containing gene from microorganisms to insects. Phylogenetic analyses revealed that NADAR genes in ladybird beetles form a well-supported clade nested within a larger group composed primarily of bacterial sequences, providing strong evidence for an HGT origin. Sampling across 69 ladybird species suggests that NADAR genes originated in the Coccinellidae family and were subsequently retained or duplicated across ladybird genomes, indicating their functional importance. Using the ladybird Cryptolaemus montrouzieri as a model, we observed that the expression levels of CmNADAR1 and CmNADAR2 were significantly upregulated in response to bacterial infection. Immune challenges combined with RNA interference targeting NADAR genes led to reduced survival rates and marked necrosis in intestinal tissues, compared to controls exposed to either bacterial infection or dsRNA alone. Together, our results demonstrate that NADAR genes in ladybird beetles were acquired through horizontal gene transfer and contribute to immune defense against bacterial infection.

水平基因转移（HGT）被广泛认为是促进宿主免疫和适应的重要机制。瓢虫有着多样化的饮食和栖息地，面临着广泛的微生物威胁，因此有效的免疫反应对它们的生存至关重要。然而，hgt获得性基因在瓢虫中的免疫作用在很大程度上仍未被探索。为了解决这一空白，我们研究了微生物和昆虫中含有NADAR （NAD-和adp -核糖相关）结构域基因的HGT。系统发育分析显示，瓢虫的NADAR基因在一个主要由细菌序列组成的更大的群体中形成了一个很好的支持分支，为HGT起源提供了有力的证据。对69种瓢虫的抽样表明，NADAR基因起源于瓢虫科，随后在瓢虫基因组中保留或复制，表明其功能重要性。以montrouzieri隐虫瓢虫为模型，我们观察到CmNADAR1和CmNADAR2的表达水平在对细菌感染的反应中显著上调。与单独暴露于细菌感染或dsRNA的对照组相比，免疫挑战结合靶向NADAR基因的RNA干扰导致肠道组织存活率降低和明显坏死。总之，我们的研究结果表明，瓢虫的NADAR基因是通过水平基因转移获得的，并有助于免疫防御细菌感染。

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引用次数: 0

Exploration of the hemocyte surfaceome of Apis mellifera by a proteomic and transcriptomic approach 用蛋白质组学和转录组学方法研究蜜蜂血细胞表面体。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-09-05 DOI: 10.1016/j.ibmb.2025.104398

Merel Braeckman , Lina De Smet , Bart Devreese , Dirk C. de Graaf

This study maps the surfaceome of Apis mellifera hemocytes, the protagonist cells in honey bee cellular immunity. The surfaceome, proteins expressed at the cell surface, is crucial as it determines how cells interact with their microenvironment. Through a combination of proteomic and transcriptomic analyses, 1142 genes encoding cell surface proteins were identified, revealing a high level of diversity. Our analyses identified receptors associated with the major insect immune pathways and proteins previously recognized as hemocyte markers in other invertebrates. Notably, several of the detected genes suggest to encode viral receptors, phagocytosis-related proteins, or proteins involved in hemocyte proliferation. A gene ontology analysis highlighted important functions of the hemocytes. The most prominent cluster was transmembrane receptor protein kinase activity, encompassing over 25 % of the identified terms. Other significant clusters included cell adhesion molecule binding, signalling receptor binding, olfactory receptor activity, and metalloendopeptidase activity. This study suggests several potential honey bee hemocyte markers and establishes a foundation for a novel hemocyte classification based on cell surface markers.

本研究绘制了蜜蜂细胞免疫的主要细胞——蜜蜂血细胞的表面体。表面体是细胞表面表达的蛋白质，它决定了细胞如何与微环境相互作用。通过蛋白质组学和转录组学的结合分析，鉴定出1142个编码细胞表面蛋白的基因，显示出高度的多样性。我们的分析确定了与主要昆虫免疫途径和蛋白质相关的受体，这些受体以前被认为是其他无脊椎动物的血细胞标记物。值得注意的是，一些检测到的基因提示编码病毒受体、吞噬相关蛋白或参与血细胞增殖的蛋白。基因本体论分析强调了血细胞的重要功能。最突出的集群是跨膜受体蛋白激酶活性，包含超过25%的已识别术语。其他重要的簇包括细胞粘附分子结合、信号受体结合、嗅觉受体活性和金属内肽酶活性。本研究提出了几种潜在的蜜蜂血细胞标记物，并为基于细胞表面标记物的新型血细胞分类奠定了基础。

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引用次数: 0

Characterizing the symbiotic relationship between Wolbachia (wSpic) and Spodoptera picta (Lepidoptera: Noctuidae): From genome to phenotype 沃尔巴克氏体（wSpic）与象形夜蛾（鳞翅目：夜蛾科）共生关系的表征：从基因组到表型

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology

Pub Date : 2025-08-27 DOI: 10.1016/j.ibmb.2025.104396

Zhixin Niu , Huizhen Guo , Dan Li , Yajing Xu , Jianqiu Liu , Yingdan Xiao , Wanshun Li , Amornrat Promboon , Qingyou Xia , Marian R. Goldsmith , Kazuei Mita

Wolbachia is a genus of symbiotic bacteria prevalent in arthropods, with diverse effects on host reproduction and fecundity; however, it is unclear how Wolbachia modulates the host reproductive system. In this study, a novel Wolbachia strain, wSpic, was identified in the Noctuid moth Spodoptera picta and its effect on the reproduction of this host was investigated. We sequenced and annotated the 1,339,720 bp genome of wSpic. We identified a total of five WO phage regions in the genome and found no evidence of any plasmids associated with wSpic. Evolutionary analysis revealed that wSpic belongs to supergroup B and has undergone horizontal transmission between S. picta and Trichogramma pretiosum, a wasp parasitoid of insect eggs. The removal of Wolbachia by antibiotic treatment resulted in significantly decreased fecundity and abnormal development of S. picta ovaries, but no differences in egg hatching rate. An integrated transcriptome and proteome analysis indicated that major molecular pathways for Wolbachia-induced reproduction fitness benefits include its effects on insect juvenile hormone, vitellogenesis, choriogenesis, and nutritional metabolism. Our findings demonstrate that wSpic plays a critical role in promoting ovary development and sustaining fecundity in S. picta hosts.

沃尔巴克氏体是一种在节肢动物中普遍存在的共生细菌属，对宿主的繁殖和繁殖力有不同的影响；然而，目前尚不清楚沃尔巴克氏体如何调节宿主的生殖系统。本研究在夜蛾（Spodoptera picta）中鉴定出一种新的沃尔巴克氏体菌株wSpic，并对其对夜蛾繁殖的影响进行了研究。我们对wSpic的1,339,720 bp基因组进行了测序和注释。我们在基因组中共鉴定了5个WO噬菌体区域，没有发现任何与wSpic相关的质粒的证据。进化分析表明，wSpic属于超B群，并在picta和蜂卵寄生蜂Trichogramma pretiosum之间进行了水平传播。抗生素处理去除沃尔巴克氏体后，雌蜂卵巢繁殖力明显下降，发育异常，但卵孵化率无显著差异。综合转录组和蛋白质组分析表明，沃尔巴克氏体诱导的生殖适应性益处的主要分子途径包括其对昆虫幼体激素、卵黄形成、绒毛膜形成和营养代谢的影响。我们的研究结果表明，wSpic在促进picta寄主卵巢发育和维持繁殖力方面起着关键作用。

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引用次数: 0