O. O. Medvid, Zh. O. Peredera, N. S. Shcherbakova, S. Peredera
Products of plant origin, recommended as permanent components of the daily human diet, are rich in vitamins, minerals, plant fibers, as well as various active phytochemicals (polyphenols, flavonoids) and sterols, which have a low calorie content. According to the results of epidemiological and clinical studies, it has been established that the risks of various pathologies are reduced when using a diet that is balanced in terms of nutrients, rich in vegetable fiber and food products of plant origin. The evolution of food technology has led to the appearance on the shelves of Italian supermarkets of products defined as “ready foods” or products – semi-finished products of high quality and safety, which present all the characteristics of freshness, similar to products that have just been harvested. They also include products of vegetable origin, which belong to the fresh-cut category. A limited technological elaboration is applied to this category of products, after which they can be used without further manipulation before consumption. The products of vegetable origin of fresh-cut vegetable have the definition of “potentially dangerous products” due to their possible contamination with pathogenic microorganisms such as Escherichia coli, Salmonella spp. and Listeria monocytogenes. These pathogens very often contaminate fresh-cut vegetable, causing food poisoning in consumers. The main sources of pathogenic contamination by the causative agents of human intestinal infections are the water used for watering plants and exposure to inappropriate temperatures during the storage of plant products. The decisive aspect, without a doubt, remains the sanitary and hygienic characteristics of the product at the time of its consumption. It is fundamental to deepen our knowledge about the transmission, resistance, and growth mechanisms of pathogenic microorganisms in products of the IV range. This will allow the establishment of sampling norms for express diagnostics to reduce the possibility of low-quality products reaching the consumer.
{"title":"EU requirements regarding hygienic and sanitary aspects of products of vegetable origin of category IV range in Italy","authors":"O. O. Medvid, Zh. O. Peredera, N. S. Shcherbakova, S. Peredera","doi":"10.32718/nvlvet10914","DOIUrl":"https://doi.org/10.32718/nvlvet10914","url":null,"abstract":"Products of plant origin, recommended as permanent components of the daily human diet, are rich in vitamins, minerals, plant fibers, as well as various active phytochemicals (polyphenols, flavonoids) and sterols, which have a low calorie content. According to the results of epidemiological and clinical studies, it has been established that the risks of various pathologies are reduced when using a diet that is balanced in terms of nutrients, rich in vegetable fiber and food products of plant origin. The evolution of food technology has led to the appearance on the shelves of Italian supermarkets of products defined as “ready foods” or products – semi-finished products of high quality and safety, which present all the characteristics of freshness, similar to products that have just been harvested. They also include products of vegetable origin, which belong to the fresh-cut category. A limited technological elaboration is applied to this category of products, after which they can be used without further manipulation before consumption. The products of vegetable origin of fresh-cut vegetable have the definition of “potentially dangerous products” due to their possible contamination with pathogenic microorganisms such as Escherichia coli, Salmonella spp. and Listeria monocytogenes. These pathogens very often contaminate fresh-cut vegetable, causing food poisoning in consumers. The main sources of pathogenic contamination by the causative agents of human intestinal infections are the water used for watering plants and exposure to inappropriate temperatures during the storage of plant products. The decisive aspect, without a doubt, remains the sanitary and hygienic characteristics of the product at the time of its consumption. It is fundamental to deepen our knowledge about the transmission, resistance, and growth mechanisms of pathogenic microorganisms in products of the IV range. This will allow the establishment of sampling norms for express diagnostics to reduce the possibility of low-quality products reaching the consumer.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43801826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A leading role in the occurrence of infertility in male domestic animals is played by nutritional deficiency factors, in particular vitamin A deficiency, which contributes to the development of morphological and functional changes in the reproductive system. The aim of the work was to determine the effect of vitamin A (carotene) deficiency on the biochemical and mineral status of infertile boars, in particular, to establish changes in the main biochemical markers and assess the state of the antioxidant defence system and indicators of mineral homeostasis (Calcium, Phosphorus, Zinc, Copper, Manganese and Cobalt). In the experimental group of animals, a probably low level of vitamin A was noted (by 54.2 % lower than the control group, P ˂ 0.001) due to a significant increase in the content of thiobarbiturate acid-reactive compounds (by 2.32 times, P ˂ 0.001) and a decrease in the activity of catalase and superoxide dismutase (by 47.3 % and 28.8 %, respectively, P ˂ 0.001). A decrease in haemoglobin concentration by 14.3 % (Р ˂ 0.001), total protein by 11.6 % (Р ˂ 0.01), the number of erythrocytes by 9.3 % (Р ˂ 0.01) and acid capacity by 9.1 % (Р ˂ 0.05). These results demonstrate a general decrease in metabolic processes in the body of infertile boars with vitamin A deficiency. The reduced number of erythrocytes in the blood of boars of the experimental group was mainly due to antiradical and antihypoxic abilities. It should be noted that catalase activity and the content of reduced glutathione in erythrocytes were probably lower than the control indicators by 53.1 % and 16.3 % (Р ˂ 0.001), respectively. In addition, taking into account the reduced indicators of haemoglobin content and the number of erythrocytes, a negative dynamics of oxygen metabolism was established. This is also confirmed by a probable increase in the content of 2,3-diphosphoglycerate in erythrocytes (by 67.5 %, Р ˂ 0.001). Also, in boars of the experimental group, the content of Calcium was lower by 25.0 % (Р ˂ 0.001) than the control indicators, and the content of Phosphorus decreased by 12.6 % (Р ˂ 0.05). Similar changes were observed in the levels of Zinc and Copper (indicators were lower than the control by 52.0 % and 28.0 %, respectively, Р ˂ 0.001). Note that the Manganese content in the blood of experimental boars was reduced by 20.9 % (Р ˂ 0.05), while the Cobalt level was lower by 16.1 % (Р ˂ 0.05). The obtained results showed a significant effect of A-vitamin deficiency on the general antioxidant activity and mineral homeostasis in barren boars.
{"title":"Biochemical and mineral status of the body of infertile boars with hypovitaminosis A","authors":"S. Naumenko, O. Miroshnikova, G. Vikulina","doi":"10.32718/nvlvet10906","DOIUrl":"https://doi.org/10.32718/nvlvet10906","url":null,"abstract":"A leading role in the occurrence of infertility in male domestic animals is played by nutritional deficiency factors, in particular vitamin A deficiency, which contributes to the development of morphological and functional changes in the reproductive system. The aim of the work was to determine the effect of vitamin A (carotene) deficiency on the biochemical and mineral status of infertile boars, in particular, to establish changes in the main biochemical markers and assess the state of the antioxidant defence system and indicators of mineral homeostasis (Calcium, Phosphorus, Zinc, Copper, Manganese and Cobalt). In the experimental group of animals, a probably low level of vitamin A was noted (by 54.2 % lower than the control group, P ˂ 0.001) due to a significant increase in the content of thiobarbiturate acid-reactive compounds (by 2.32 times, P ˂ 0.001) and a decrease in the activity of catalase and superoxide dismutase (by 47.3 % and 28.8 %, respectively, P ˂ 0.001). A decrease in haemoglobin concentration by 14.3 % (Р ˂ 0.001), total protein by 11.6 % (Р ˂ 0.01), the number of erythrocytes by 9.3 % (Р ˂ 0.01) and acid capacity by 9.1 % (Р ˂ 0.05). These results demonstrate a general decrease in metabolic processes in the body of infertile boars with vitamin A deficiency. The reduced number of erythrocytes in the blood of boars of the experimental group was mainly due to antiradical and antihypoxic abilities. It should be noted that catalase activity and the content of reduced glutathione in erythrocytes were probably lower than the control indicators by 53.1 % and 16.3 % (Р ˂ 0.001), respectively. In addition, taking into account the reduced indicators of haemoglobin content and the number of erythrocytes, a negative dynamics of oxygen metabolism was established. This is also confirmed by a probable increase in the content of 2,3-diphosphoglycerate in erythrocytes (by 67.5 %, Р ˂ 0.001). Also, in boars of the experimental group, the content of Calcium was lower by 25.0 % (Р ˂ 0.001) than the control indicators, and the content of Phosphorus decreased by 12.6 % (Р ˂ 0.05). Similar changes were observed in the levels of Zinc and Copper (indicators were lower than the control by 52.0 % and 28.0 %, respectively, Р ˂ 0.001). Note that the Manganese content in the blood of experimental boars was reduced by 20.9 % (Р ˂ 0.05), while the Cobalt level was lower by 16.1 % (Р ˂ 0.05). The obtained results showed a significant effect of A-vitamin deficiency on the general antioxidant activity and mineral homeostasis in barren boars.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44529959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the conditions for successfully managing rabbit breeding is ensuring the epizootological well-being of farms concerning invasive animal diseases. Helminthiases, protozooses, and acaroses cause significant economic damage to the industry due to a decrease in the biological value of rabbit meat and skins, a delay in growth, development, and a decrease in the growth of young animals, as well as their death. Carrying out epizootic monitoring and assessment of the epizootic situation in different regions of Ukraine is one of the main preventive measures, which, based on the results of the study of the epizootic state, analysis of veterinary reports, and the results of laboratory tests, will allow effective treatment and prevention of invasive diseases in rabbit farms. The purpose of the work was to conduct monitoring studies of the epizootic situation regarding rabbit parasitoses in the territory of some areas of Ukraine. It was established that the total incidence of domestic rabbits by parasitoses was 36.27 %. The weight of infestations caused by the simplest organisms was the highest, reaching 81.96 %. The specific weight of infestations caused by acariform mites, nematodes, and cestodes was insignificant and amounted to 9.88 %, 7.3 %, and 0.86 %, respectively. Among the protozooses – eimeriosis, nematodoses – pasalurosis, cestodoses – cysticercosis, acaroses –psoroptosis were found in rabbits. High indicators of the extensiveness of animal invasion were established for eimeriosis (50.23 %). Smaller values of infestation of rabbits were found for psoroptosis (18.0 %) and pasalurosis (16.87 %). For pysiform cysticercosis, the extent of invasion was insignificant – 5.97 %. Indicators of the extensiveness of invasions in different regions of Ukraine ranged from 15.28 to 100.0 % for eimeriosis, from 4.35 to 100.0 % for pasalurosis, from 50.0 to 100.0 % for cysticercosis, and from psoroptosis 11.54 to 100.0 %. The obtained results of monitoring studies on the epizootic status of invasive rabbit diseases on the territory of Ukraine indicate the relevance and perspective of further studying the distribution of parasite fauna in the population of domestic rabbits, taking into account the climatic conditions of different regions, the way rabbits are kept, their age and season.
{"title":"Epizootological monitoring of rabbit parasitoses on the territory of Ukraine","authors":"B. Gutyj, O. Boyko, L. Korchan","doi":"10.32718/nvlvet10901","DOIUrl":"https://doi.org/10.32718/nvlvet10901","url":null,"abstract":"One of the conditions for successfully managing rabbit breeding is ensuring the epizootological well-being of farms concerning invasive animal diseases. Helminthiases, protozooses, and acaroses cause significant economic damage to the industry due to a decrease in the biological value of rabbit meat and skins, a delay in growth, development, and a decrease in the growth of young animals, as well as their death. Carrying out epizootic monitoring and assessment of the epizootic situation in different regions of Ukraine is one of the main preventive measures, which, based on the results of the study of the epizootic state, analysis of veterinary reports, and the results of laboratory tests, will allow effective treatment and prevention of invasive diseases in rabbit farms. The purpose of the work was to conduct monitoring studies of the epizootic situation regarding rabbit parasitoses in the territory of some areas of Ukraine. It was established that the total incidence of domestic rabbits by parasitoses was 36.27 %. The weight of infestations caused by the simplest organisms was the highest, reaching 81.96 %. The specific weight of infestations caused by acariform mites, nematodes, and cestodes was insignificant and amounted to 9.88 %, 7.3 %, and 0.86 %, respectively. Among the protozooses – eimeriosis, nematodoses – pasalurosis, cestodoses – cysticercosis, acaroses –psoroptosis were found in rabbits. High indicators of the extensiveness of animal invasion were established for eimeriosis (50.23 %). Smaller values of infestation of rabbits were found for psoroptosis (18.0 %) and pasalurosis (16.87 %). For pysiform cysticercosis, the extent of invasion was insignificant – 5.97 %. Indicators of the extensiveness of invasions in different regions of Ukraine ranged from 15.28 to 100.0 % for eimeriosis, from 4.35 to 100.0 % for pasalurosis, from 50.0 to 100.0 % for cysticercosis, and from psoroptosis 11.54 to 100.0 %. The obtained results of monitoring studies on the epizootic status of invasive rabbit diseases on the territory of Ukraine indicate the relevance and perspective of further studying the distribution of parasite fauna in the population of domestic rabbits, taking into account the climatic conditions of different regions, the way rabbits are kept, their age and season.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44514367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most types of helminths are widespread in many countries and severely threaten human health. One of the most dangerous zooanthropohelminthiases is toxocarosis. The causative agent of toxocarosis – the nematode Toxocara canis in the imaginal stage parasitizes in the small intestine of carnivores and the larval stage (Visceralis larva migrans) – in various organs and tissues of animals and humans. Toxocarosis has both veterinary and social significance. Toxocara canis larvae can migrate in the human body and cause various pathologies: hepatitis, encephalitis, chorioretinitis. The purpose of the research was to analyze data from the reporting documentation of the Poltava Veterinary Center regarding the prevalence of toxocarosis among the domestic dog population. According to the results of the analysis of the statistical data of the reporting documentation of the veterinary center of the Shevchenkivskyi district of the city of Poltava for 2019–2022, it was established that the average infestation of dogs by the causative agent of toxocarosis in the territory of the city of Poltava is 22.89 %, with a range from 17.51 to 28.02 %. The share of toxocarosis in the total parasitic pathology diagnosed in dogs was 27 %. The mestizos were the most affected by the causative agent of toxocarosis, where the extent of invasion was 29.26 %. A lower rate of toxocares was found in dogs of decorative (24.05 %), service (21.24 %), and hunting (18.56 %) breeds. In particular, the highest values of the extent of infestation were found in Yorkshire terriers (39.71 %). Less often, toxocarosis was diagnosed in toy terriers (extent of infestation – 19.12 %), English cocker spaniels (19.83 %), dachshunds (18.03 %), pugs (16.37 %), Jack Russell terriers (15.82 %) and pickiness (12.6 %). Seasonal dynamics of toxocarosis in dogs are characterized by the peak of invasion in the spring and autumn periods, where the infection rates of animals were at 35.0 and 30.0 %, respectively. The extent of toxocarosis infestation declined in winter – by 12.0 %. The obtained data indicate the relevance of the problem of toxocarosis as a zooanthroponous invasion in connection with the significant damage to domestic dogs by toxocares in the city of Poltava.
{"title":"Monitoring studies of the spread of toxocarosis in dogs in the city of Poltava","authors":"H. Pohorelova","doi":"10.32718/nvlvet10912","DOIUrl":"https://doi.org/10.32718/nvlvet10912","url":null,"abstract":"Most types of helminths are widespread in many countries and severely threaten human health. One of the most dangerous zooanthropohelminthiases is toxocarosis. The causative agent of toxocarosis – the nematode Toxocara canis in the imaginal stage parasitizes in the small intestine of carnivores and the larval stage (Visceralis larva migrans) – in various organs and tissues of animals and humans. Toxocarosis has both veterinary and social significance. Toxocara canis larvae can migrate in the human body and cause various pathologies: hepatitis, encephalitis, chorioretinitis. The purpose of the research was to analyze data from the reporting documentation of the Poltava Veterinary Center regarding the prevalence of toxocarosis among the domestic dog population. According to the results of the analysis of the statistical data of the reporting documentation of the veterinary center of the Shevchenkivskyi district of the city of Poltava for 2019–2022, it was established that the average infestation of dogs by the causative agent of toxocarosis in the territory of the city of Poltava is 22.89 %, with a range from 17.51 to 28.02 %. The share of toxocarosis in the total parasitic pathology diagnosed in dogs was 27 %. The mestizos were the most affected by the causative agent of toxocarosis, where the extent of invasion was 29.26 %. A lower rate of toxocares was found in dogs of decorative (24.05 %), service (21.24 %), and hunting (18.56 %) breeds. In particular, the highest values of the extent of infestation were found in Yorkshire terriers (39.71 %). Less often, toxocarosis was diagnosed in toy terriers (extent of infestation – 19.12 %), English cocker spaniels (19.83 %), dachshunds (18.03 %), pugs (16.37 %), Jack Russell terriers (15.82 %) and pickiness (12.6 %). Seasonal dynamics of toxocarosis in dogs are characterized by the peak of invasion in the spring and autumn periods, where the infection rates of animals were at 35.0 and 30.0 %, respectively. The extent of toxocarosis infestation declined in winter – by 12.0 %. The obtained data indicate the relevance of the problem of toxocarosis as a zooanthroponous invasion in connection with the significant damage to domestic dogs by toxocares in the city of Poltava.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47411754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heliceculture (breeding of snails) has been practiced in Europe for a long time, and here, in Ukraine, the first snail farms appeared literally 10 years ago. Ukrainians quickly realized that among all areas of animal husbandry, heliceculture is the most profitable. At the same time, you can start a business almost from scratch, and the risks are minimized. This is how the snail boom spread throughout the territory, and now molluscs are grown in almost all cities of Ukraine. It should be remembered veterinary and sanitary requirements on snail farms must be given important attention, because if they are violated, it can lead to the death of molluscs and cause great losses. Taking into account the importance of growing snails in farms and the lack of regulatory and technical documentation in our country, the purpose of this work was to clarify the main veterinary and sanitary requirements for such farms. In this work, the general veterinary and sanitary rules for snail breeding farms, requirements for water and feed, preparation for transportation, vehicles, types of containers, packaging materials and requirements for keeping snails in the field in the summer are carefully considered. Owners of snail farming farms are obliged to implement economic and veterinary-sanitary measures that ensure the prevention of the death of molluscs, maintain premises and facilities for storing feed and processing snail-farming products in proper condition, prevent environmental pollution with waste, comply with zoohygienic and veterinary-sanitary requirements for placement, construction, commissioning of objects related to keeping snails. Compliance with these requirements in the farm plays a big role in the cultivation and maintenance of snails. This will make it possible to grow snails, avoiding any diseases that are inherent to them and death, which will make it possible to obtain snail farming products of high quality and safety. Therefore, in order to prevent invasive diseases, snail poisoning, etc., managers and specialists of these farms (farms) are obliged to ensure the implementation of a set of general veterinary and sanitary measures, as well as the implementation of veterinary and sanitary requirements for the construction, equipment, operation of snail farms and their compliance sanitary regime.
{"title":"Veterinary and sanitary requirements for snail farms","authors":"I. Danilova, T. Danilova","doi":"10.32718/nvlvet10905","DOIUrl":"https://doi.org/10.32718/nvlvet10905","url":null,"abstract":"Heliceculture (breeding of snails) has been practiced in Europe for a long time, and here, in Ukraine, the first snail farms appeared literally 10 years ago. Ukrainians quickly realized that among all areas of animal husbandry, heliceculture is the most profitable. At the same time, you can start a business almost from scratch, and the risks are minimized. This is how the snail boom spread throughout the territory, and now molluscs are grown in almost all cities of Ukraine. It should be remembered veterinary and sanitary requirements on snail farms must be given important attention, because if they are violated, it can lead to the death of molluscs and cause great losses. Taking into account the importance of growing snails in farms and the lack of regulatory and technical documentation in our country, the purpose of this work was to clarify the main veterinary and sanitary requirements for such farms. In this work, the general veterinary and sanitary rules for snail breeding farms, requirements for water and feed, preparation for transportation, vehicles, types of containers, packaging materials and requirements for keeping snails in the field in the summer are carefully considered. Owners of snail farming farms are obliged to implement economic and veterinary-sanitary measures that ensure the prevention of the death of molluscs, maintain premises and facilities for storing feed and processing snail-farming products in proper condition, prevent environmental pollution with waste, comply with zoohygienic and veterinary-sanitary requirements for placement, construction, commissioning of objects related to keeping snails. Compliance with these requirements in the farm plays a big role in the cultivation and maintenance of snails. This will make it possible to grow snails, avoiding any diseases that are inherent to them and death, which will make it possible to obtain snail farming products of high quality and safety. Therefore, in order to prevent invasive diseases, snail poisoning, etc., managers and specialists of these farms (farms) are obliged to ensure the implementation of a set of general veterinary and sanitary measures, as well as the implementation of veterinary and sanitary requirements for the construction, equipment, operation of snail farms and their compliance sanitary regime.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49562712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. P. Goralskyi, I. Sokulskyi, N. Kolesnik, O. Dunaievska, N. L. Radzykhovskyi, B. Gutyj, S. Shevchuk
An important issue of modern neuromorphology of animals is the study of spinal cord nodes, which play an important role as primary centers on the way to transmit sensory information from receptors to the central nervous system, providing appropriate reactions to the action of certain factors. The scientific article uses material that is a fragment of the research work of the department of normal and pathological morphology, hygiene and expertise “Development, morphology and histochemistry of animal organs in normal and pathological conditions”, state registration number – 0120U100796. Using anatomical, morphological, neurohistological and statistical methods of research, the histomorphology of spinal cord nodes in a comparative anatomical series of vertebrates: bony fish, amphibians, reptiles, which differ in motor activity and their place of existence in a certain environment, was clarified in the work. It has been established that in the process of phylogenesis, a certain structural and morphofunctional reorganization of the spinal nodes occurs. They differ in shape and size. Adaptation to various living conditions of animals was formed on the basis of changes in the density and size of neurons, their morphological structures, an increase in the total number of gliocytes and glial cells, and polymorphism in the degree of chromatophilia. Thus, according to neurohistological studies, it has been noted that the neurons of the spinal cord nodes of cold-blooded animals (pond frog, pond lizard) are characterized by a functional degree of relative polymorphism – chromatophilia. With the total impregnation of the spinal cord nodes with silver nitrate according to the Bolshovsky-Gross method, different intensity of staining of nerve cells is found in all the studied animals: light, light-dark and dark. It was found that the neurocytes of all groups of vertebrates differ in the volume of the perikaryon, the nucleus and the nuclear-cytoplasmic ratio, which is related to the peculiarities of species and age-related neuromorphology, the morpho-functional state of the nervous system and the type of higher nervous activity. Nissl staining of histopreparations of spinal nodes of poikilothermic animals showed that the neuroplasm of nerve cells of spinal nodes contains deep basophilic substances in the form of small grains of varying density. In carp neurons, the basophilic substance is concentrated on the periphery of the dust-like neuroplasm. Nuclear chromatin is more pronounced in the pond frog than in the carp, the depths of which are much larger. It should be noted that the highest degree of development of the protein-synthesizing apparatus is characteristic of the neurocytes of the spinal nodes of the swift lizard, compared to the previously studied vertebrates. The obtained research results have not only important general biological significance, which complements and expands modern scientific ideas about certain regularities of morpho-functional rearrang
{"title":"Evolutionary morphology of spinal nodes of poikilotherm vertebrate animals","authors":"L. P. Goralskyi, I. Sokulskyi, N. Kolesnik, O. Dunaievska, N. L. Radzykhovskyi, B. Gutyj, S. Shevchuk","doi":"10.32718/nvlvet10908","DOIUrl":"https://doi.org/10.32718/nvlvet10908","url":null,"abstract":"An important issue of modern neuromorphology of animals is the study of spinal cord nodes, which play an important role as primary centers on the way to transmit sensory information from receptors to the central nervous system, providing appropriate reactions to the action of certain factors. The scientific article uses material that is a fragment of the research work of the department of normal and pathological morphology, hygiene and expertise “Development, morphology and histochemistry of animal organs in normal and pathological conditions”, state registration number – 0120U100796. Using anatomical, morphological, neurohistological and statistical methods of research, the histomorphology of spinal cord nodes in a comparative anatomical series of vertebrates: bony fish, amphibians, reptiles, which differ in motor activity and their place of existence in a certain environment, was clarified in the work. It has been established that in the process of phylogenesis, a certain structural and morphofunctional reorganization of the spinal nodes occurs. They differ in shape and size. Adaptation to various living conditions of animals was formed on the basis of changes in the density and size of neurons, their morphological structures, an increase in the total number of gliocytes and glial cells, and polymorphism in the degree of chromatophilia. Thus, according to neurohistological studies, it has been noted that the neurons of the spinal cord nodes of cold-blooded animals (pond frog, pond lizard) are characterized by a functional degree of relative polymorphism – chromatophilia. With the total impregnation of the spinal cord nodes with silver nitrate according to the Bolshovsky-Gross method, different intensity of staining of nerve cells is found in all the studied animals: light, light-dark and dark. It was found that the neurocytes of all groups of vertebrates differ in the volume of the perikaryon, the nucleus and the nuclear-cytoplasmic ratio, which is related to the peculiarities of species and age-related neuromorphology, the morpho-functional state of the nervous system and the type of higher nervous activity. Nissl staining of histopreparations of spinal nodes of poikilothermic animals showed that the neuroplasm of nerve cells of spinal nodes contains deep basophilic substances in the form of small grains of varying density. In carp neurons, the basophilic substance is concentrated on the periphery of the dust-like neuroplasm. Nuclear chromatin is more pronounced in the pond frog than in the carp, the depths of which are much larger. It should be noted that the highest degree of development of the protein-synthesizing apparatus is characteristic of the neurocytes of the spinal nodes of the swift lizard, compared to the previously studied vertebrates. The obtained research results have not only important general biological significance, which complements and expands modern scientific ideas about certain regularities of morpho-functional rearrang","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45306550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Masliuk, O. Orobchenko, M. Romanko, Y. Koreneva, V. Klochkov, S. Yefimova, N. Kavok
The prohibition of using antibiotics with growth-stimulating properties in the European Union led to the search for new, safer, more effective, and cheaper feed additives. One of these substances was rare earth elements (REE, atomic numbers 57–71) due to their low toxicity and protective and antioxidant properties. At the same time, higher efficiency of organic forms of REE was noted. Thanks to this, it is possible to assume their even more pronounced effect in nano-sized form as feed additives and the need to conduct research in this direction. Therefore, this work aims to study the metabolic parameters of the blood in rats under long-term oral administration of nanoparticles of one of the representatives of rare earth elements – gadolinium orthovanadate (NP GdVO4:Eu3+) under food stress. Experimental samples of gadolinium orthovanadate nanoparticles activated by Europium (spindle-shaped geometry; size 8×25 nm; initial concentration 1.0 g/dm3) were used in work. Experimental studies on rats were carried out based on the vivarium of the NSC “IEKVM”. The object of research was 140 mature male Wistar rats with an initial weight of 180–200 g. Four groups of animals, 35 rats each, were formed according to the principle of analogs. During the experiment, animals of the control group received drinking water without additives; rats of the І experimental group were given a solution of NP GdVO4:Eu3+ at a dose of 0.2 mg/dm3 (≈ 0.03 mg/kg of body weight); II research group – at a dose of 1.0 mg/dm3 (≈ 0.15 mg/kg of body weight) and rats of III research group – at a dose of 2.0 mg/dm3 (≈ 0.30 mg/kg of body weight). Drinking was carried out for 56 days, then it was completed, and the rats were observed for another 14 days. A nutritionally unbalanced diet was used as a stress factor. Taking into account the results of biochemical studies, the adaptogenic effect of NP GdVO4:Eu3+ in the range of doses of 0.2–1.0 mg/dm3 of drinking water (≈ 0.03–0.15 mg/kg of body weight) on the body of white rats was established under conditions of food stress with optimal duration of action – 28–42 days. Under the conditions of administration at a dose of 2.0 mg/dm3 of drinking water (≈ 0.30 mg/kg of body weight), a hepatic(cyto-)toxic effect of nanoparticles was detected, which was accompanied by an irreversible decrease in the structural indicators of lipid metabolism, the consumption of antioxidant resources and the induction of intensity processes of lipid peroxidation against the background of alanine aminotransferase hyperenzymemia.
{"title":"The state of metabolic parameters of the blood in white rats under conditions of long-term oral administration of gadolinium orthovanadate nanoparticles under food stress","authors":"A. Masliuk, O. Orobchenko, M. Romanko, Y. Koreneva, V. Klochkov, S. Yefimova, N. Kavok","doi":"10.32718/nvlvet10911","DOIUrl":"https://doi.org/10.32718/nvlvet10911","url":null,"abstract":"The prohibition of using antibiotics with growth-stimulating properties in the European Union led to the search for new, safer, more effective, and cheaper feed additives. One of these substances was rare earth elements (REE, atomic numbers 57–71) due to their low toxicity and protective and antioxidant properties. At the same time, higher efficiency of organic forms of REE was noted. Thanks to this, it is possible to assume their even more pronounced effect in nano-sized form as feed additives and the need to conduct research in this direction. Therefore, this work aims to study the metabolic parameters of the blood in rats under long-term oral administration of nanoparticles of one of the representatives of rare earth elements – gadolinium orthovanadate (NP GdVO4:Eu3+) under food stress. Experimental samples of gadolinium orthovanadate nanoparticles activated by Europium (spindle-shaped geometry; size 8×25 nm; initial concentration 1.0 g/dm3) were used in work. Experimental studies on rats were carried out based on the vivarium of the NSC “IEKVM”. The object of research was 140 mature male Wistar rats with an initial weight of 180–200 g. Four groups of animals, 35 rats each, were formed according to the principle of analogs. During the experiment, animals of the control group received drinking water without additives; rats of the І experimental group were given a solution of NP GdVO4:Eu3+ at a dose of 0.2 mg/dm3 (≈ 0.03 mg/kg of body weight); II research group – at a dose of 1.0 mg/dm3 (≈ 0.15 mg/kg of body weight) and rats of III research group – at a dose of 2.0 mg/dm3 (≈ 0.30 mg/kg of body weight). Drinking was carried out for 56 days, then it was completed, and the rats were observed for another 14 days. A nutritionally unbalanced diet was used as a stress factor. Taking into account the results of biochemical studies, the adaptogenic effect of NP GdVO4:Eu3+ in the range of doses of 0.2–1.0 mg/dm3 of drinking water (≈ 0.03–0.15 mg/kg of body weight) on the body of white rats was established under conditions of food stress with optimal duration of action – 28–42 days. Under the conditions of administration at a dose of 2.0 mg/dm3 of drinking water (≈ 0.30 mg/kg of body weight), a hepatic(cyto-)toxic effect of nanoparticles was detected, which was accompanied by an irreversible decrease in the structural indicators of lipid metabolism, the consumption of antioxidant resources and the induction of intensity processes of lipid peroxidation against the background of alanine aminotransferase hyperenzymemia.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45350493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Dubova, D. Feshchenko, O. Zghozinska, A. Dubovyi
The article presents the results of studying the course of some nematodoses in turtles and tortoises kept in terrariums. Helminth infections account for 35 % of all parasitic diseases of reptiles and are a significant danger, especially after wintering and for weakened individuals. The purpose of the work is to establish the helminth fauna of turtles and clinical testing of the topical application of an anthelmintic on a lipophilic basis. Research methods are coprological, statistical. The greatest extensiveness and intensity of invasion were found in nematodes Tachygonetria spp. Wedi, 1862 (family Oxyuridae) and Angusticaecum spp. Baylis, 1920 (family Ascaridae). Identified pathogens under the conditions of wintering in terrariums and after it are able to cause manifested forms of the disease in connection with the stress experienced by turtles. Invasion of Tachigonetria spp. manifested by gastrointestinal disorders: diarrhea, regurgitation of feed, weakness. Parasitism of Angusticaecum spp. characterized in addition to disorders of the digestive system by signs of respiratory pathology: shortness of breath of the expiratory type, secretion of serous and serous-purulent exudate, snoring, coughing. The highest intensity of invasion by both genera of nematodes was found in the spring, in the period after wintering, and the lowest – in the summer, in the season of the hottest weather. During a clinical trial of the use of various anthelmintics and different ways of administering the drug, it was established that Panacur orally and Profender topically have high intensive efficacy, however, 100 % extensive efficacy was not found in both drugs. Topical application of Profender on a lipophilic basis showed better results of intensity, especially against Angusticaecum spp., than enteral administration of Panacur. High, but incomplete intensity effectiveness of both drugs against Tachigonetria spp. is explained by the extraordinary spread of the causative agent's eggs in the environment, their pronounced resistance to extreme environmental conditions, commensalism of nematodes. Preparations on a lipophilic basis for parenteral (topical) use have prospects for use in reptile veterinary medicine.
{"title":"Peculiarities of the some nematodoses course in turtles and tortoises under conditions of keeping in terrariums","authors":"O. Dubova, D. Feshchenko, O. Zghozinska, A. Dubovyi","doi":"10.32718/nvlvet10920","DOIUrl":"https://doi.org/10.32718/nvlvet10920","url":null,"abstract":"The article presents the results of studying the course of some nematodoses in turtles and tortoises kept in terrariums. Helminth infections account for 35 % of all parasitic diseases of reptiles and are a significant danger, especially after wintering and for weakened individuals. The purpose of the work is to establish the helminth fauna of turtles and clinical testing of the topical application of an anthelmintic on a lipophilic basis. Research methods are coprological, statistical. The greatest extensiveness and intensity of invasion were found in nematodes Tachygonetria spp. Wedi, 1862 (family Oxyuridae) and Angusticaecum spp. Baylis, 1920 (family Ascaridae). Identified pathogens under the conditions of wintering in terrariums and after it are able to cause manifested forms of the disease in connection with the stress experienced by turtles. Invasion of Tachigonetria spp. manifested by gastrointestinal disorders: diarrhea, regurgitation of feed, weakness. Parasitism of Angusticaecum spp. characterized in addition to disorders of the digestive system by signs of respiratory pathology: shortness of breath of the expiratory type, secretion of serous and serous-purulent exudate, snoring, coughing. The highest intensity of invasion by both genera of nematodes was found in the spring, in the period after wintering, and the lowest – in the summer, in the season of the hottest weather. During a clinical trial of the use of various anthelmintics and different ways of administering the drug, it was established that Panacur orally and Profender topically have high intensive efficacy, however, 100 % extensive efficacy was not found in both drugs. Topical application of Profender on a lipophilic basis showed better results of intensity, especially against Angusticaecum spp., than enteral administration of Panacur. High, but incomplete intensity effectiveness of both drugs against Tachigonetria spp. is explained by the extraordinary spread of the causative agent's eggs in the environment, their pronounced resistance to extreme environmental conditions, commensalism of nematodes. Preparations on a lipophilic basis for parenteral (topical) use have prospects for use in reptile veterinary medicine.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42238101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Sokolyuk, I. Ligomina, V. Dukhnytskyi, P. Boyko, V. Dzhmil, N. Boltyk
In the modern conditions of dairy farming, the presence of indigestible foreign bodies in the stomachs of cattle is common, which is due to many factors. In 15 cows of the farm (6 % of the total number of animals in the herd), culled due to the pathology of the digestive organs, damage to the mesh and adjacent organs was diagnosed. The largest number of indigestible foreign bodies was metal objects, which were found in 8 cows; nylon ropes, kapron nets, polyethylene bags – for 4 cows; rags and leather items – from 2 cows; gravel and pieces of concrete were found in one animal. Consumption of indigestible foreign bodies is a common occurrence in farmed cattle, which can be a significant cause of loss of productivity, premature culling, and even animal death. Proper handling of indigestible foreign objects, plastic waste on the farm should be an integral part of preventive measures for animal disease, reduction of culling and improvement of animal welfare. Comprehensive dispensation must be carried out in the following areas: determination of herd syndromes, clinical studies of animals, laboratory studies of blood, milk, urine, analysis of feeding and keeping of animals. One of the main preventive measures is the regular probing of cows for metal-carrying objects with magnetic probes, the introduction of metal object catchers. Among the workers serving the livestock industry, carry out explanatory work on the danger of spreading various indigestible objects on the territory of fodder lands and livestock farms.
{"title":"Indigesible foreign bodies in feedfor livestock and preventive measures in farm conditions","authors":"V. Sokolyuk, I. Ligomina, V. Dukhnytskyi, P. Boyko, V. Dzhmil, N. Boltyk","doi":"10.32718/nvlvet10915","DOIUrl":"https://doi.org/10.32718/nvlvet10915","url":null,"abstract":"In the modern conditions of dairy farming, the presence of indigestible foreign bodies in the stomachs of cattle is common, which is due to many factors. In 15 cows of the farm (6 % of the total number of animals in the herd), culled due to the pathology of the digestive organs, damage to the mesh and adjacent organs was diagnosed. The largest number of indigestible foreign bodies was metal objects, which were found in 8 cows; nylon ropes, kapron nets, polyethylene bags – for 4 cows; rags and leather items – from 2 cows; gravel and pieces of concrete were found in one animal. Consumption of indigestible foreign bodies is a common occurrence in farmed cattle, which can be a significant cause of loss of productivity, premature culling, and even animal death. Proper handling of indigestible foreign objects, plastic waste on the farm should be an integral part of preventive measures for animal disease, reduction of culling and improvement of animal welfare. Comprehensive dispensation must be carried out in the following areas: determination of herd syndromes, clinical studies of animals, laboratory studies of blood, milk, urine, analysis of feeding and keeping of animals. One of the main preventive measures is the regular probing of cows for metal-carrying objects with magnetic probes, the introduction of metal object catchers. Among the workers serving the livestock industry, carry out explanatory work on the danger of spreading various indigestible objects on the territory of fodder lands and livestock farms.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46539369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Despite the benefits and biological value of dairy products, they can also be a source of dangerous substances of natural and artificial origin. Such substances include the estrogen hormone 17β-estradiol, an excessive amount of which causes oncological diseases and reproductive system dysfunction. A study was conducted to determine the toxicological evaluation of raw milk with different 17β-estradiol content on the culture of the ciliate Tetrachymena pyriformis. The content of 17β-estradiol was determined by enzyme immunoassay using the RIDASCREEN®17β-őstradiol test system (Art-Biopharm/R-Biopharm, Darmstadt, Germany). During the determination of acute toxicity, under the condition of the content of 17ß-estradiol in the raw milk in the amount from 20.0 to 2500 pg/ml, no changes in motor activity, body shape and inhibition of reproduction of ciliates were observed. At the content of 17ß-estradiol in milk in the range of 5000.0−5500.0 pg/ml, a slowdown in the motor activity of ciliates was observed without changing the nature of the movement. Thus, the amount of 17ß-estradiol in milk greater than 5000.0 pg/ml causes a minor effect on the vital activity and motor activity of Tetrachymena pyriformis ciliates. During 96 hours of the existence of ciliates in milk, chronic toxic effects on cells were not observed for the content of 17ß-estradiol from 20.0 to 2500.0 pg/ml. Addition of 17ß-estradiol to raw milk in the amount of 5000.0 to 5500.0 pg/ml caused a chronic toxic effect on ciliates, as a result of which the total score in all determined indicators increased to 2 points. Thus, raw milk with an amount of 17ß-estradiol from 5000.0 to 5500.0 pg/ml may exhibit some chronic toxic effect on the cells of the ciliated infusoria. It was established that the relative biological value of raw milk with a content of 17ß-estradiol up to 2500.0 pg/ml was from 98.6 to 97.7 %, and in samples of raw milk with the maximum amount of the hormone (5000.0−5500.0 pg/ml) decreased by an average of 10 % to 89.22 %. Therefore, if the amount of 17ß-estradiol in milk is more than 5000.0 mg/ml, a chronic toxic effect on Tetrachymena pyriformis cells is possible.
{"title":"Toxicological characteristics of raw milk with different contents of 17β-estradiol","authors":"","doi":"10.32718/nvlvet10904","DOIUrl":"https://doi.org/10.32718/nvlvet10904","url":null,"abstract":"Despite the benefits and biological value of dairy products, they can also be a source of dangerous substances of natural and artificial origin. Such substances include the estrogen hormone 17β-estradiol, an excessive amount of which causes oncological diseases and reproductive system dysfunction. A study was conducted to determine the toxicological evaluation of raw milk with different 17β-estradiol content on the culture of the ciliate Tetrachymena pyriformis. The content of 17β-estradiol was determined by enzyme immunoassay using the RIDASCREEN®17β-őstradiol test system (Art-Biopharm/R-Biopharm, Darmstadt, Germany). During the determination of acute toxicity, under the condition of the content of 17ß-estradiol in the raw milk in the amount from 20.0 to 2500 pg/ml, no changes in motor activity, body shape and inhibition of reproduction of ciliates were observed. At the content of 17ß-estradiol in milk in the range of 5000.0−5500.0 pg/ml, a slowdown in the motor activity of ciliates was observed without changing the nature of the movement. Thus, the amount of 17ß-estradiol in milk greater than 5000.0 pg/ml causes a minor effect on the vital activity and motor activity of Tetrachymena pyriformis ciliates. During 96 hours of the existence of ciliates in milk, chronic toxic effects on cells were not observed for the content of 17ß-estradiol from 20.0 to 2500.0 pg/ml. Addition of 17ß-estradiol to raw milk in the amount of 5000.0 to 5500.0 pg/ml caused a chronic toxic effect on ciliates, as a result of which the total score in all determined indicators increased to 2 points. Thus, raw milk with an amount of 17ß-estradiol from 5000.0 to 5500.0 pg/ml may exhibit some chronic toxic effect on the cells of the ciliated infusoria. It was established that the relative biological value of raw milk with a content of 17ß-estradiol up to 2500.0 pg/ml was from 98.6 to 97.7 %, and in samples of raw milk with the maximum amount of the hormone (5000.0−5500.0 pg/ml) decreased by an average of 10 % to 89.22 %. Therefore, if the amount of 17ß-estradiol in milk is more than 5000.0 mg/ml, a chronic toxic effect on Tetrachymena pyriformis cells is possible.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41373364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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