Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-07
Y. E. Rozin
Objective. To conduct a comparative analysis of hemodynamic parameters during orthopedic and traumatological operations on the lower extremities in children performed under conditions of multicomponent balanced general and combined anesthesia. Materials and methods. A single-center prospective study. The present study included 60 children aged 6 to 17 years who underwent planned orthopedic and trauma surgery interventions on the lower extremities. Patients were divided into two groups depending on the method of anesthesia: Group 1 (n = 25) - multicomponent balanced general anesthesia, Group 2 (n = 35) - combined anesthesia (multicomponent balanced general anesthesia in combination with regional blocks of the sciatic and femoral nerves). To solve these problems, a comparative analysis of heart rate, systolic, diastolic and mean arterial blood pressure was carried out at seven stages of the study: 1st stage - initial, child on the operating table; 2nd stage - induction of anesthesia; 3rd stage - placement of the laryngeal mask; 4th stage - start of the operation; 5th stage - traumatic stage of the operation; 6th stage - completion of the operation; 7th stage - after removal of the laryngeal mask. Results. In all cases, the surgical interventions were successful. The study found that in the group of children who underwent multicomponent general anesthesia at the 4th (start of surgery), 5th (traumatic stage of surgery) and 6th (end of surgery) stages, heart rate (HR) was higher compared to the group of combined anesthesia. Systolic and mean arterial blood pressure during the traumatic phase of surgery were also higher in children undergoing multicomponent general anesthesia. The values of diastolic blood pressure did not differ between groups throughout the study with the exception of the 3rd stage (laryngeal mask placement) where lower values were observed in patients of the Group 1. Diastolic blood pressure values did not differ between groups throughout the study, with the exception of 3th stage (laryngeal mask placement), where lower values were noted in Group 1 patients. Conclusion. Combined methods of anesthesia based on the combination of multicomponent general anesthesia and conductive peripheral nerve blocks provide the greatest hemodynamic stability during orthopedic and traumatologic surgeries on the lower extremities in children.
{"title":"Comparative assessment of hemodynamic parameters during anesthesia in orthopedic and traumatological operations on the lower extremities in children","authors":"Y. E. Rozin","doi":"10.51523/2708-6011.2024-21-1-07","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-07","url":null,"abstract":" Objective. To conduct a comparative analysis of hemodynamic parameters during orthopedic and traumatological operations on the lower extremities in children performed under conditions of multicomponent balanced general and combined anesthesia. Materials and methods. A single-center prospective study. The present study included 60 children aged 6 to 17 years who underwent planned orthopedic and trauma surgery interventions on the lower extremities. Patients were divided into two groups depending on the method of anesthesia: Group 1 (n = 25) - multicomponent balanced general anesthesia, Group 2 (n = 35) - combined anesthesia (multicomponent balanced general anesthesia in combination with regional blocks of the sciatic and femoral nerves). To solve these problems, a comparative analysis of heart rate, systolic, diastolic and mean arterial blood pressure was carried out at seven stages of the study: 1st stage - initial, child on the operating table; 2nd stage - induction of anesthesia; 3rd stage - placement of the laryngeal mask; 4th stage - start of the operation; 5th stage - traumatic stage of the operation; 6th stage - completion of the operation; 7th stage - after removal of the laryngeal mask. Results. In all cases, the surgical interventions were successful. The study found that in the group of children who underwent multicomponent general anesthesia at the 4th (start of surgery), 5th (traumatic stage of surgery) and 6th (end of surgery) stages, heart rate (HR) was higher compared to the group of combined anesthesia. Systolic and mean arterial blood pressure during the traumatic phase of surgery were also higher in children undergoing multicomponent general anesthesia. The values of diastolic blood pressure did not differ between groups throughout the study with the exception of the 3rd stage (laryngeal mask placement) where lower values were observed in patients of the Group 1. Diastolic blood pressure values did not differ between groups throughout the study, with the exception of 3th stage (laryngeal mask placement), where lower values were noted in Group 1 patients. Conclusion. Combined methods of anesthesia based on the combination of multicomponent general anesthesia and conductive peripheral nerve blocks provide the greatest hemodynamic stability during orthopedic and traumatologic surgeries on the lower extremities in children.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"19 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-10
N. Puzan, I. A. Cheshik, V. N. Belyakovsky
Objective. To study in vitro the effect of therapeutic doses of radiation on the albumin molecule using intrinsic and probe fluorescence methods. Materials and methods. In order to study radiation-induced changes in serum albumin during in vitro irradiation with therapeutic doses (2 Gy, 40 Gy and 70 Gy), the study was conducted in 2 directions: therapeutic doses of ionizing radiation irradiated a buffer, which was then used to prepare an albumin solution (pre-irradiation of the buffer); therapeutic doses of ionizing radiation irradiated an albumin buffer solution. The presence of structural and functional (conformational) changes in the albumin molecule was judged by changes in the values of intrinsic (λexc=280 nm) and probe (λexc=280nm, λexc=320 nm) fluorescence. Statistical processing of the obtained data was carried out using the program GraphPad Prism 6.0. Results. Irradiation with therapeutic doses of 2 Gy, 40 Gy and 70 Gy causes conformational changes (a statistically significant decrease in fluorescence intensity) in the albumin molecule, both during preliminary irradiation of the buffer solution used later for the preparation of albumin, and during irradiation of the buffer solution of albumin. Conclusions. Quantitative changes in the fluorescence intensity, both intrinsic and probe, differ under different modes of albumin irradiation.
{"title":"Methods of intrinsic and probe fluorescence in assessment of the effect of therapeutic radiation doses in vitro on the albumin molecule","authors":"N. Puzan, I. A. Cheshik, V. N. Belyakovsky","doi":"10.51523/2708-6011.2024-21-1-10","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-10","url":null,"abstract":" Objective. To study in vitro the effect of therapeutic doses of radiation on the albumin molecule using intrinsic and probe fluorescence methods. Materials and methods. In order to study radiation-induced changes in serum albumin during in vitro irradiation with therapeutic doses (2 Gy, 40 Gy and 70 Gy), the study was conducted in 2 directions: therapeutic doses of ionizing radiation irradiated a buffer, which was then used to prepare an albumin solution (pre-irradiation of the buffer); therapeutic doses of ionizing radiation irradiated an albumin buffer solution. The presence of structural and functional (conformational) changes in the albumin molecule was judged by changes in the values of intrinsic (λexc=280 nm) and probe (λexc=280nm, λexc=320 nm) fluorescence. Statistical processing of the obtained data was carried out using the program GraphPad Prism 6.0. Results. Irradiation with therapeutic doses of 2 Gy, 40 Gy and 70 Gy causes conformational changes (a statistically significant decrease in fluorescence intensity) in the albumin molecule, both during preliminary irradiation of the buffer solution used later for the preparation of albumin, and during irradiation of the buffer solution of albumin. Conclusions. Quantitative changes in the fluorescence intensity, both intrinsic and probe, differ under different modes of albumin irradiation.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"7 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-16
N. N. Spoda, O. L. Nikiforova, D. S. Gorbach, N. V. Lin
Systemic connective tissue diseases are a heterogeneous group of diseases with unclear etiology, the pathogenesis of which is represented by an autoimmune process, a heterogeneous clinical picture and a variable course. Classical variants of the disease that do not cause difficulties in their verification are most common in clinical practice. But some patients have signs characteristic of various connective tissue diseases. This combination of symptoms is called mixed connective tissue disease or “cross syndrome” (Overlap-syndrome). The multi-organ nature of the clinical picture of the disease presents a diagnostic difficulty for the clinician and leads to late diagnosis, ineffective therapy, and disability of the patient. The article presents a clinical case of mixed connective tissue disease in a middle-aged patient. An analysis of literary sources, clinical data, and laboratory results was carried out.
{"title":"Clinical observation of a patient with mixed connective tissue disease in an outpatient practice","authors":"N. N. Spoda, O. L. Nikiforova, D. S. Gorbach, N. V. Lin","doi":"10.51523/2708-6011.2024-21-1-16","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-16","url":null,"abstract":" Systemic connective tissue diseases are a heterogeneous group of diseases with unclear etiology, the pathogenesis of which is represented by an autoimmune process, a heterogeneous clinical picture and a variable course. Classical variants of the disease that do not cause difficulties in their verification are most common in clinical practice. But some patients have signs characteristic of various connective tissue diseases. This combination of symptoms is called mixed connective tissue disease or “cross syndrome” (Overlap-syndrome). The multi-organ nature of the clinical picture of the disease presents a diagnostic difficulty for the clinician and leads to late diagnosis, ineffective therapy, and disability of the patient. The article presents a clinical case of mixed connective tissue disease in a middle-aged patient. An analysis of literary sources, clinical data, and laboratory results was carried out.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"27 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-08
V. Ziamko, A. Dzyadzko
Objective. To study the effect of drugs regulating acute immune responses on liver in septic processes. Materials and methods. The study was conducted on 39 male white rats weighing 250-400 g. The animals were initiated septic process development by intraperitoneal injection of Klebsiella pneumoniae culture suspension with simultaneous intravenous injection of polyvinylpyrrolidone (PVP) and gadolinium in the volume of 0.6 ml. On the 14th day the animals were removed from the experiment by decapitation under light ether anesthesia, blood and liver were taken for biochemical and histological studies. Results. When analyzing the morphometric parameters of liver preparations, an increase in the number of nuclei and a slight increase in the area of nuclei in three experimental groups was established by 1,2 times (p < 0,05) compared to the control group, which reflects the ongoing processes of hepatocyte regeneration due to possible mechanisms, namely hypertrophy nuclei and proliferation with both PVP and gadolinium in systemic inflammatory response and sepsis. Moreover, the use of PVP and gadolinium led to a decrease in the likelihood of perinuclear edema, protein degeneration and large droplet vacuolization (p < 0,05). When using gadolinium, the lumen diameter of the sinusoids was the largest and amounted to 4,47; 3,22-5,63 µm (p<0,05), and in septic shock it did not differ from the group where PVP was used (p > 0,05). Of the laboratory parameters of surviving individuals, the lowest ALaT level was noted in the gadolinium experimental group – 53,7; 51,8-55,1 U/L (p<0,05), while the lowest urea level was observed when using PVP (5,0; 4,99-5,15 mmol/l) (p < 0,05) as part of a systemic inflammatory response. Conclusion. Gadolinium and PVP have a positive effect on the detoxification function of liver. Moreover, the effect of PVP on the morphology and function of the liver differs at the stages of septic process.
{"title":"Effect of drugs regulating acute immune responses on the liver in septic processes","authors":"V. Ziamko, A. Dzyadzko","doi":"10.51523/2708-6011.2024-21-1-08","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-08","url":null,"abstract":" Objective. To study the effect of drugs regulating acute immune responses on liver in septic processes. Materials and methods. The study was conducted on 39 male white rats weighing 250-400 g. The animals were initiated septic process development by intraperitoneal injection of Klebsiella pneumoniae culture suspension with simultaneous intravenous injection of polyvinylpyrrolidone (PVP) and gadolinium in the volume of 0.6 ml. On the 14th day the animals were removed from the experiment by decapitation under light ether anesthesia, blood and liver were taken for biochemical and histological studies. Results. When analyzing the morphometric parameters of liver preparations, an increase in the number of nuclei and a slight increase in the area of nuclei in three experimental groups was established by 1,2 times (p < 0,05) compared to the control group, which reflects the ongoing processes of hepatocyte regeneration due to possible mechanisms, namely hypertrophy nuclei and proliferation with both PVP and gadolinium in systemic inflammatory response and sepsis. Moreover, the use of PVP and gadolinium led to a decrease in the likelihood of perinuclear edema, protein degeneration and large droplet vacuolization (p < 0,05). When using gadolinium, the lumen diameter of the sinusoids was the largest and amounted to 4,47; 3,22-5,63 µm (p<0,05), and in septic shock it did not differ from the group where PVP was used (p > 0,05). Of the laboratory parameters of surviving individuals, the lowest ALaT level was noted in the gadolinium experimental group – 53,7; 51,8-55,1 U/L (p<0,05), while the lowest urea level was observed when using PVP (5,0; 4,99-5,15 mmol/l) (p < 0,05) as part of a systemic inflammatory response. Conclusion. Gadolinium and PVP have a positive effect on the detoxification function of liver. Moreover, the effect of PVP on the morphology and function of the liver differs at the stages of septic process.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"25 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140359875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-17
I. D. Shlyaga, M. O. Miazheinikava, N. P. Cheliabiyeva, I. P. Hlavatskaya, G. V. Tishchenko, A. A. Suharev
The article describes a clinical case of atypical aggressive progression of mycosis-associated hyperplasia of the lingual tonsil with proliferative changes in the pharyngeal and palatine tonsils. The features of etiology, pathogenesis, diagnostic challenges, and an effective treatment method for this pathology are presented. Based on clinical experience, it is important to note that the prevalence of lingual tonsil diseases is significantly higher than their detectability. This is attributed to its anatomical-topographical and morphological characteristics of location and structure, as well as the relative difficulty of examination (due to a high pharyngeal reflex), the need for the specialist physician to master hypopharyngoscopy techniques, and the lack of a clear algorithm for clinical examination of such patients.
{"title":"Clinical case of aggressive course of hyperplasia of the lingual tonsil","authors":"I. D. Shlyaga, M. O. Miazheinikava, N. P. Cheliabiyeva, I. P. Hlavatskaya, G. V. Tishchenko, A. A. Suharev","doi":"10.51523/2708-6011.2024-21-1-17","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-17","url":null,"abstract":" The article describes a clinical case of atypical aggressive progression of mycosis-associated hyperplasia of the lingual tonsil with proliferative changes in the pharyngeal and palatine tonsils. The features of etiology, pathogenesis, diagnostic challenges, and an effective treatment method for this pathology are presented. Based on clinical experience, it is important to note that the prevalence of lingual tonsil diseases is significantly higher than their detectability. This is attributed to its anatomical-topographical and morphological characteristics of location and structure, as well as the relative difficulty of examination (due to a high pharyngeal reflex), the need for the specialist physician to master hypopharyngoscopy techniques, and the lack of a clear algorithm for clinical examination of such patients.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"31 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-13
V. S. Volchek, T. Sharshakova, D. M. Los’
Objective. To analyze the time series of morbidity and mortality from breast cancer, prostate cancer, colorectal cancer and cervical cancer in Gomel region for the period from 1982 to 2022 using ARIMA method and to make a prediction up to 2030. Materials and methods. We analyzed morbidity and mortality data from these cancers using statistical methods, including ARIMA modeling. Data on age at diagnosis, stage of disease, percentages of cancer detected at different stages, percentages of mortality and its dynamics after diagnosis were used. Results. On the basis of data analysis using ARIMA model, a model for predicting the incidence of cancer in the near future was developed. According to the forecast, it should be expected that there will be an increase in the incidence of breast, prostate, cervical and colorectal cancer until 2030, a decrease in the age of patients at first detection, a change in the pattern of detection at different stages and an increase in the percentage of disease detection in preventive examinations. Mortality from these types of cancer is projected to increase, with some changes in the average age of patientsof death indicators and stage dynamics. Conclusion. Predicting cancer incidence and mortality rates emphasizes the need for increased screening and early diagnosis of malignant neoplasms. This can improve earlier detection of the disease and increase the effectiveness of treatment, reducing overall cancer mortality.
{"title":"Dynamics of morbidity and mortality from cancer in Gomel region: statistical analysis and forecast using ARIMA model up to 2030","authors":"V. S. Volchek, T. Sharshakova, D. M. Los’","doi":"10.51523/2708-6011.2024-21-1-13","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-13","url":null,"abstract":" Objective. To analyze the time series of morbidity and mortality from breast cancer, prostate cancer, colorectal cancer and cervical cancer in Gomel region for the period from 1982 to 2022 using ARIMA method and to make a prediction up to 2030. Materials and methods. We analyzed morbidity and mortality data from these cancers using statistical methods, including ARIMA modeling. Data on age at diagnosis, stage of disease, percentages of cancer detected at different stages, percentages of mortality and its dynamics after diagnosis were used. Results. On the basis of data analysis using ARIMA model, a model for predicting the incidence of cancer in the near future was developed. According to the forecast, it should be expected that there will be an increase in the incidence of breast, prostate, cervical and colorectal cancer until 2030, a decrease in the age of patients at first detection, a change in the pattern of detection at different stages and an increase in the percentage of disease detection in preventive examinations. Mortality from these types of cancer is projected to increase, with some changes in the average age of patientsof death indicators and stage dynamics. Conclusion. Predicting cancer incidence and mortality rates emphasizes the need for increased screening and early diagnosis of malignant neoplasms. This can improve earlier detection of the disease and increase the effectiveness of treatment, reducing overall cancer mortality.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"14 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-15
I. A. Novikova, A. A. Ramaniva, S. A. Khoduleva, N. G. Kadochkina, S. S. Prokopovich
The article presents a case of common variable immunodeficiency without clinically significant infectious syndrome with manifestation in the form of Crohn’s disease. The article emphasizes the expediency of including in the complex of examination in chronic inflammatory diseases of the gastrointestinal tract the test for assessing the level of immunoglobulins of the main classes in serum.
{"title":"Crohn’s disease as a debut of common variable immunodeficiency","authors":"I. A. Novikova, A. A. Ramaniva, S. A. Khoduleva, N. G. Kadochkina, S. S. Prokopovich","doi":"10.51523/2708-6011.2024-21-1-15","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-15","url":null,"abstract":" The article presents a case of common variable immunodeficiency without clinically significant infectious syndrome with manifestation in the form of Crohn’s disease. The article emphasizes the expediency of including in the complex of examination in chronic inflammatory diseases of the gastrointestinal tract the test for assessing the level of immunoglobulins of the main classes in serum.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140359673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-18
O. L. Nikiforova, O. Osipkina, N. V. Galinovskaya, E. Voropaev
Objective. To assess the state of the systemic inflammatory response and humoral immune response in middle-aged patients came through COVID-19 infection in mild and moderate severity. Materials and methods. A cohort single-center study involving 83 patients came through COVID-19 infection in mild and moderate severity (women - 69.9% (58 patients), men - 30.1% (25 patients); median age 53 years [49; 56] was conducted over the period 2022-2023. All patients underwent clinical examination, evaluation of humoral response with determination of IgG and IgM antibodies to SARS-CoV-2 virus, as well as concentration of interleukins: interleukin-1β, interleukin-4, interleukin-6, interleukin-8, interleukin-10, high-sensitivity C-reactive protein, tumour necrosis factor α, transforming growth factor β1 and procalcitonin concentrations. Patients were divided into three groups: 1-3 months; 3-6 months; and 6 months to 1 year after COVID-19 infection. Results. Within a year the percentage of IgM-positive patients in all patient groups was about a quarter (25 %, 21 % and 25 % respectively). While remaining within the reference values for the whole group, procalcitonin and interleukin-10 levels in the selected cohort exceeded those of the test-negative cohort (p = 0.0053 and p = 0.0044, respectively), which we considered as persistent chronic compensated inflammation. Conclusion. A quarter of patients who came through COVID-19 infection in mild and moderate severity have persistently positive IgM levels for a year, combined with proinflammatory status according to systemic inflammatory response parameters.
{"title":"Interactions of humoral immune response indicators and parameters of systemic inflammatory response in patients with post-COVID-19 syndrome","authors":"O. L. Nikiforova, O. Osipkina, N. V. Galinovskaya, E. Voropaev","doi":"10.51523/2708-6011.2024-21-1-18","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-18","url":null,"abstract":" Objective. To assess the state of the systemic inflammatory response and humoral immune response in middle-aged patients came through COVID-19 infection in mild and moderate severity. Materials and methods. A cohort single-center study involving 83 patients came through COVID-19 infection in mild and moderate severity (women - 69.9% (58 patients), men - 30.1% (25 patients); median age 53 years [49; 56] was conducted over the period 2022-2023. All patients underwent clinical examination, evaluation of humoral response with determination of IgG and IgM antibodies to SARS-CoV-2 virus, as well as concentration of interleukins: interleukin-1β, interleukin-4, interleukin-6, interleukin-8, interleukin-10, high-sensitivity C-reactive protein, tumour necrosis factor α, transforming growth factor β1 and procalcitonin concentrations. Patients were divided into three groups: 1-3 months; 3-6 months; and 6 months to 1 year after COVID-19 infection. Results. Within a year the percentage of IgM-positive patients in all patient groups was about a quarter (25 %, 21 % and 25 % respectively). While remaining within the reference values for the whole group, procalcitonin and interleukin-10 levels in the selected cohort exceeded those of the test-negative cohort (p = 0.0053 and p = 0.0044, respectively), which we considered as persistent chronic compensated inflammation. Conclusion. A quarter of patients who came through COVID-19 infection in mild and moderate severity have persistently positive IgM levels for a year, combined with proinflammatory status according to systemic inflammatory response parameters.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"112 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-09
E. Pashinskaya, I. S. Sobolevskaya, A. K. Pashinskaya, I. V. Ignateva, V. Pobyarzhin, S. L. Sobolevsky, K. A. Chicherova
Objective. To assess the significance of light chronodestruction in the development of the embryotoxic effect in the experiment. Materials and methods. The experiment was conducted on 60 female rats of the Wistar line. To assess the significance of chronodestruction in the development of embryotoxic effect, the uterus was isolated in the experiment, in which the number of implantation sites, the total number of embryos, the number of live and dead embryos, and the number of resorptions were estimated. The number of corpus luteum in the harvested ovaries was estimated. In addition, the mean embryo weight (g) and mean craniocaudal size (mm) were recorded. Indicators of embryotoxic effects of light deprivation were determined by pre- and post-implantation death, which was calculated in accordance with methodological recommendations. Results. Exposure to light deprivation reduces the number of implantation sites as of 7th, 14th and 21st days by 1,5-1,8 times, the total number of embryos by 1,6-1,8 times, the number of living embryos by 2,2-9 times and increases the number of dead embryos as of 14th and 21st days by 4-5,5 times, the number of resorptions – 1,6-11 times. The average craniocaudal size (mm) of embryos in experimental animals was recorded below control values by 1,5 times, 1,3 and 3,7 times by 7th, 14th, 21st days respectively. In females exposed to light deprivation, there was a significant increase in pre-implantation mortality by 35-41,8 times and post-implantation mortality by 7,2-20,4 times compared to the control. Conclusion. Light deprivation may have a negative effect on pregnancy and fetal development in female rats, which is confirmed by an increase in pre- and post-implantation mortality.
{"title":"The significance of light chronodestruction in the development of the embryotoxic effect in the experiment","authors":"E. Pashinskaya, I. S. Sobolevskaya, A. K. Pashinskaya, I. V. Ignateva, V. Pobyarzhin, S. L. Sobolevsky, K. A. Chicherova","doi":"10.51523/2708-6011.2024-21-1-09","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-09","url":null,"abstract":" Objective. To assess the significance of light chronodestruction in the development of the embryotoxic effect in the experiment. Materials and methods. The experiment was conducted on 60 female rats of the Wistar line. To assess the significance of chronodestruction in the development of embryotoxic effect, the uterus was isolated in the experiment, in which the number of implantation sites, the total number of embryos, the number of live and dead embryos, and the number of resorptions were estimated. The number of corpus luteum in the harvested ovaries was estimated. In addition, the mean embryo weight (g) and mean craniocaudal size (mm) were recorded. Indicators of embryotoxic effects of light deprivation were determined by pre- and post-implantation death, which was calculated in accordance with methodological recommendations. Results. Exposure to light deprivation reduces the number of implantation sites as of 7th, 14th and 21st days by 1,5-1,8 times, the total number of embryos by 1,6-1,8 times, the number of living embryos by 2,2-9 times and increases the number of dead embryos as of 14th and 21st days by 4-5,5 times, the number of resorptions – 1,6-11 times. The average craniocaudal size (mm) of embryos in experimental animals was recorded below control values by 1,5 times, 1,3 and 3,7 times by 7th, 14th, 21st days respectively. In females exposed to light deprivation, there was a significant increase in pre-implantation mortality by 35-41,8 times and post-implantation mortality by 7,2-20,4 times compared to the control. Conclusion. Light deprivation may have a negative effect on pregnancy and fetal development in female rats, which is confirmed by an increase in pre- and post-implantation mortality.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"6 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-31DOI: 10.51523/2708-6011.2024-21-1-12
L. V. Lagun, Ya. A. Kulvinsky, N. A. Kulvinskaya
Objective. To evaluate the sensitivity of methicillin-resistant Staphylococcus aureus strains to antibiotics and therapeutic and prophylactic preparations of bacteriophages. Materials and methods. The antibiotic sensitivity of strains of methicillin-resistant S.aureus was studied by the disco-diffusion method, the determination of the minimum inhibitory concentrations of vancomycin and linezolid by broth microdilution method, and ceftaroline by E-tests. The sensitivity of methicillin-resistant S.aureus to bacteriophage preparations was studied. Results. It has been established that linezolid and vancomycin are quite active drugs against methicillin-resistant S.aureus (MRSA), however, resistance to them begins to develop. MRSA resistance to fluoroquinolones and cefoxitin was the highest of all antibiotics tested; the results obtained with cefoxitin can be interpreted for other cephalosporins (except ceftaroline) and all penicillins. Only 67.9% of MRSA strains retained sensitivity to ceftaroline, but ceftaroline-resistant strains had low levels of minimal inhibitory concentration. The preparations of the bacteriophages “Sextaphage” and “Piobacteriophage Phagio” showed their lytic activity only in relation to 35.8% and 18.9% of MRSA isolates, respectively. Conclusion. Monitoring the development of antibiotic resistance in methicillin-resistant S.aureus is an important part of the strategy of rational antibiotic therapy for staphylococcal infections. Only taking into account the preliminary step-by-step determination of the phage sensitivity of the causative agent of the disease, bacteriophage preparations can be considered as a completely possible alternative to antibiotics for the treatment of purulent inflammatory diseases caused by MRSA.
{"title":"Study of the sensitivity of methicillin-resistant Staphylococcus aureus to antibiotics and bacteriophage preparations","authors":"L. V. Lagun, Ya. A. Kulvinsky, N. A. Kulvinskaya","doi":"10.51523/2708-6011.2024-21-1-12","DOIUrl":"https://doi.org/10.51523/2708-6011.2024-21-1-12","url":null,"abstract":" Objective. To evaluate the sensitivity of methicillin-resistant Staphylococcus aureus strains to antibiotics and therapeutic and prophylactic preparations of bacteriophages. Materials and methods. The antibiotic sensitivity of strains of methicillin-resistant S.aureus was studied by the disco-diffusion method, the determination of the minimum inhibitory concentrations of vancomycin and linezolid by broth microdilution method, and ceftaroline by E-tests. The sensitivity of methicillin-resistant S.aureus to bacteriophage preparations was studied. Results. It has been established that linezolid and vancomycin are quite active drugs against methicillin-resistant S.aureus (MRSA), however, resistance to them begins to develop. MRSA resistance to fluoroquinolones and cefoxitin was the highest of all antibiotics tested; the results obtained with cefoxitin can be interpreted for other cephalosporins (except ceftaroline) and all penicillins. Only 67.9% of MRSA strains retained sensitivity to ceftaroline, but ceftaroline-resistant strains had low levels of minimal inhibitory concentration. The preparations of the bacteriophages “Sextaphage” and “Piobacteriophage Phagio” showed their lytic activity only in relation to 35.8% and 18.9% of MRSA isolates, respectively. Conclusion. Monitoring the development of antibiotic resistance in methicillin-resistant S.aureus is an important part of the strategy of rational antibiotic therapy for staphylococcal infections. Only taking into account the preliminary step-by-step determination of the phage sensitivity of the causative agent of the disease, bacteriophage preparations can be considered as a completely possible alternative to antibiotics for the treatment of purulent inflammatory diseases caused by MRSA.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"12 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：