Pub Date : 2023-12-07DOI: 10.22141/2224-0551.18.7.2023.1647
A. Abaturov, V. Babуch
The scientific review considers the mechanisms of microRNA regulation of biological processes in the human body with the help of food products, namely those of animal origin. To write the article, information was searched using Scopus, Web of Science, MEDLINE, PubMed, Google Scholar, Embase, Global Health, The Cochrane Library databases. It is known that microRNA molecules of milk retain their biological activity in the digestive tract for a long time, reach the intestinal mucosa and penetrate the internal continuum of the body. It is stated that in breast milk, microRNAs are mainly found in extracellular vesicles, which are signalosomes that mediate the effectiveness of molecular communication between the mother and her child. Breast milk has been shown to contain about 1,400 different miRNAs, most of which are located in exosomes. The authors indicate that the representation of miRNA in breast milk changes during the postpartum period. Scientists believe that large amounts of microRNAs are found both in raw cow’s milk and in commercial dairy foods. Formulas are miRNA-deficient dairy foods. The authors provide data that miR-148a deficiency is associated with the development of pathological processes of the hepatobiliary system such as inflammation, liver fibrosis, carcinogenesis and lipid metabolism disorders. It has been shown that meat products contain large amounts of various miRNAs, which can retain their functional activity even after heat treatment. So, in a comprehensive review using the latest information search databases, it was found that in the modern scientific literature, the authors determine the horizontal transfer of numerous microRNA molecules from animals to the human body. The main food product that restores microRNA deficiency is milk. Breast milk contains mRNA, microRNA and many other active substances. Feeding children with formulas leads to a pronounced deficiency of exogenous miRNAs. The change in the structure of the human transcriptome is due to the consumption of meat products.
该科学综述考虑了microRNA在食品(即动物源性食品)的帮助下调控人体生物过程的机制。为了撰写这篇文章，我们使用Scopus、Web of Science、MEDLINE、PubMed、Google Scholar、Embase、Global Health、Cochrane Library等数据库进行了信息搜索。众所周知，牛奶中的微rna分子在消化道内长期保持生物活性，到达肠黏膜，穿透机体内部连续体。研究表明，在母乳中，microrna主要存在于细胞外囊泡中，它是介导母婴分子通讯有效性的信号体。母乳含有大约1400种不同的mirna，其中大部分位于外泌体中。作者指出，miRNA在母乳中的表达在产后发生了变化。科学家认为，在生牛奶和商业乳制品中都发现了大量的microrna。配方奶是缺乏mirna的乳制品。作者提供的数据表明，miR-148a缺乏与肝胆系统的病理过程的发展有关，如炎症、肝纤维化、致癌和脂质代谢紊乱。研究表明，肉制品中含有大量的各种mirna，即使经过热处理也能保持其功能活性。因此，在使用最新信息检索数据库的综合回顾中，发现在现代科学文献中，作者确定了许多microRNA分子从动物到人体的水平转移。恢复microRNA缺乏的主要食品是牛奶。母乳中含有mRNA、microRNA和许多其他活性物质。用配方奶粉喂养儿童会导致外源性mirna的明显缺乏。人类转录组结构的变化是由于食用肉制品。
{"title":"Regulation of microRNA with food. Part 2. Food of animal origin","authors":"A. Abaturov, V. Babуch","doi":"10.22141/2224-0551.18.7.2023.1647","DOIUrl":"https://doi.org/10.22141/2224-0551.18.7.2023.1647","url":null,"abstract":"The scientific review considers the mechanisms of microRNA regulation of biological processes in the human body with the help of food products, namely those of animal origin. To write the article, information was searched using Scopus, Web of Science, MEDLINE, PubMed, Google Scholar, Embase, Global Health, The Cochrane Library databases. It is known that microRNA molecules of milk retain their biological activity in the digestive tract for a long time, reach the intestinal mucosa and penetrate the internal continuum of the body. It is stated that in breast milk, microRNAs are mainly found in extracellular vesicles, which are signalosomes that mediate the effectiveness of molecular communication between the mother and her child. Breast milk has been shown to contain about 1,400 different miRNAs, most of which are located in exosomes. The authors indicate that the representation of miRNA in breast milk changes during the postpartum period. Scientists believe that large amounts of microRNAs are found both in raw cow’s milk and in commercial dairy foods. Formulas are miRNA-deficient dairy foods. The authors provide data that miR-148a deficiency is associated with the development of pathological processes of the hepatobiliary system such as inflammation, liver fibrosis, carcinogenesis and lipid metabolism disorders. It has been shown that meat products contain large amounts of various miRNAs, which can retain their functional activity even after heat treatment. So, in a comprehensive review using the latest information search databases, it was found that in the modern scientific literature, the authors determine the horizontal transfer of numerous microRNA molecules from animals to the human body. The main food product that restores microRNA deficiency is milk. Breast milk contains mRNA, microRNA and many other active substances. Feeding children with formulas leads to a pronounced deficiency of exogenous miRNAs. The change in the structure of the human transcriptome is due to the consumption of meat products.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"116 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.22141/2224-0551.18.7.2023.1646
L.M. Bulat, O. Lysunets, N. Didyk
The problem of coronavirus disease (COVID) requires further study and generalization because the pathogenetic mechanisms are not fully elucidated, and the clinical data are varied. Therefore, it is necessary to accumulate data about the features of coronavirus disease in patients of different age groups in order to enrich the clinical experience. The treatment and diagnosis outcomes were used to make a conclusion about clinical manifestation in pediatric COVID patients. The data were extracted using PubMed/MEDLINE and Google Scholar databases. Generally, important components of coronavirus disease pathogenesis are viral tropism for cell host, cytokine storm, endothelial dysfunction, hemodynamic instability, and dehydration. These pathogenic ways lead to multiorgan injury, so clinical signs of coronavirus infection might be different. It depends on a system that suffers from coronavirus. However, pediatric COVID infection can be mild with respiratory signs, or it can be a multisystem inflammatory syndrome. On the other hand, there are typically laboratory features of coronavirus disease, including a significant rise in inflammatory and coagulation markers such as C-reactive protein, procalcitonin, fibrinogen and D-dimer. Moreover, coronavirus has displaced other expected etiological factors of some acute upper and lower respiratory tract infections and caused acute stenosis laryngitis, bronchiolitis, or pneumonia. Interestingly, coronavirus might be a cause of Kawasaki disease, therefore, physicians should pay attention to all clinical and laboratory signs in children in order to make a clear decision about diagnosis and treatment. The benefits of this study consist in presentation of pediatric COVID features. Modern knowledge will allow to choose necessary diagnostic test in time. General practitioners will be able to refer the patients to hospital before complication arise.
{"title":"Clinical variants of the coronavirus disease in children (review of literature)","authors":"L.M. Bulat, O. Lysunets, N. Didyk","doi":"10.22141/2224-0551.18.7.2023.1646","DOIUrl":"https://doi.org/10.22141/2224-0551.18.7.2023.1646","url":null,"abstract":"The problem of coronavirus disease (COVID) requires further study and generalization because the pathogenetic mechanisms are not fully elucidated, and the clinical data are varied. Therefore, it is necessary to accumulate data about the features of coronavirus disease in patients of different age groups in order to enrich the clinical experience. The treatment and diagnosis outcomes were used to make a conclusion about clinical manifestation in pediatric COVID patients. The data were extracted using PubMed/MEDLINE and Google Scholar databases. Generally, important components of coronavirus disease pathogenesis are viral tropism for cell host, cytokine storm, endothelial dysfunction, hemodynamic instability, and dehydration. These pathogenic ways lead to multiorgan injury, so clinical signs of coronavirus infection might be different. It depends on a system that suffers from coronavirus. However, pediatric COVID infection can be mild with respiratory signs, or it can be a multisystem inflammatory syndrome. On the other hand, there are typically laboratory features of coronavirus disease, including a significant rise in inflammatory and coagulation markers such as C-reactive protein, procalcitonin, fibrinogen and D-dimer. Moreover, coronavirus has displaced other expected etiological factors of some acute upper and lower respiratory tract infections and caused acute stenosis laryngitis, bronchiolitis, or pneumonia. Interestingly, coronavirus might be a cause of Kawasaki disease, therefore, physicians should pay attention to all clinical and laboratory signs in children in order to make a clear decision about diagnosis and treatment. The benefits of this study consist in presentation of pediatric COVID features. Modern knowledge will allow to choose necessary diagnostic test in time. General practitioners will be able to refer the patients to hospital before complication arise.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"54 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138593044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.22141/2224-0551.18.7.2023.1644
PhD M.Z. Lisiecka
Background. The importance of issue is the rising incidence of allergies to volatile organic compounds, particularly in children and adults. This necessitates understanding the causes, risk factors, and methods for prevention and treatment. The purpose of the study was to clarify the relationship between the development of allergy to lipid transfer proteins (LTP) and various environmental influences, nutrition and the immune state of the body. Materials and methods. To achieve goal, the following research methods were used: content analysis of the database of medical organizations, the method of a representative sample and quantitative examination. In the practical part of the study, we used the Immuno Solid-phase Allergen Chip test. Results. The study results demonstrated adverse reactions in both children and adults with LTP allergies. Peach (Prunus persica) was identified as an allergenic product. It was found that childhood is a particularly sensitive period for the development of allergic reactions to LTP due to incomplete immune development. Additionally, the peculiarities of food allergy were investigated, taking into account its connection with antibodies and cause-and-effect relationships. Conclusions. Sensitization to lipid transfer proteins occurs in various groups, including children and adults, and depends on the sources of exposure.
{"title":"Development of allergy to LTP in children and adults","authors":"PhD M.Z. Lisiecka","doi":"10.22141/2224-0551.18.7.2023.1644","DOIUrl":"https://doi.org/10.22141/2224-0551.18.7.2023.1644","url":null,"abstract":"Background. The importance of issue is the rising incidence of allergies to volatile organic compounds, particularly in children and adults. This necessitates understanding the causes, risk factors, and methods for prevention and treatment. The purpose of the study was to clarify the relationship between the development of allergy to lipid transfer proteins (LTP) and various environmental influences, nutrition and the immune state of the body. Materials and methods. To achieve goal, the following research methods were used: content analysis of the database of medical organizations, the method of a representative sample and quantitative examination. In the practical part of the study, we used the Immuno Solid-phase Allergen Chip test. Results. The study results demonstrated adverse reactions in both children and adults with LTP allergies. Peach (Prunus persica) was identified as an allergenic product. It was found that childhood is a particularly sensitive period for the development of allergic reactions to LTP due to incomplete immune development. Additionally, the peculiarities of food allergy were investigated, taking into account its connection with antibodies and cause-and-effect relationships. Conclusions. Sensitization to lipid transfer proteins occurs in various groups, including children and adults, and depends on the sources of exposure.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"10 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.22141/2224-0551.18.7.2023.1643
K. Z. Kyzy, K. Sakibaev, A. Sattarov, G. Dzholdosheva, E. Zholdosheva, PhD K. Sakibaev
Background. Improving pediatric observation globally and in Kyrgyzstan is essential for early detection of developmental issues and monitoring normal physical parameters. The purpose of the study was to investigate the indicators of bone composition in children of Kyrgyz nationality of both sexes in the early childhood period. Materials and methods. To achieve the goal, 800 children from 4 to 7 years old were examined using anatomical-anthropometric and bioimpedance methods. Results. It was revealed that the absolute content of bone component in male respondents at the age of 4 years ranges from 3.24 to 5.17 (4.10 ± 0.01) kg, 5 years — from 3.27 to 5.81 (4.26 ± 0.01) kg, 6 years — from 3.34 to 5.87 (4.39 ± 0.01) kg and 7 years — from 3.23 up to 6.02 (4.59 ± 0.01) kg. The absolute index of the bone component in 4-year-old girls has an individual variability of 3.04–5.17 (3.97 ± 0.01) kg, 5-year-old — 3.27–5.61 (4.20 ± 0.01) kg, 6-year-old — 3.34–5.77 (4.34 ± 0.01) kg and 7-year-old — 3.43–6.02 (4.40 ± 0.01) kg. Conclusions. The highest bone content was found in individuals with muscular somatotype, while the lowest values were in asthenoid and thoracic somatotypes for both males and females. Boys had higher bone component index at ages of 4 and 7.
{"title":"Absolute content of bone component in the body of Kyrgyz children with different somatotypes in the early childhood","authors":"K. Z. Kyzy, K. Sakibaev, A. Sattarov, G. Dzholdosheva, E. Zholdosheva, PhD K. Sakibaev","doi":"10.22141/2224-0551.18.7.2023.1643","DOIUrl":"https://doi.org/10.22141/2224-0551.18.7.2023.1643","url":null,"abstract":"Background. Improving pediatric observation globally and in Kyrgyzstan is essential for early detection of developmental issues and monitoring normal physical parameters. The purpose of the study was to investigate the indicators of bone composition in children of Kyrgyz nationality of both sexes in the early childhood period. Materials and methods. To achieve the goal, 800 children from 4 to 7 years old were examined using anatomical-anthropometric and bioimpedance methods. Results. It was revealed that the absolute content of bone component in male respondents at the age of 4 years ranges from 3.24 to 5.17 (4.10 ± 0.01) kg, 5 years — from 3.27 to 5.81 (4.26 ± 0.01) kg, 6 years — from 3.34 to 5.87 (4.39 ± 0.01) kg and 7 years — from 3.23 up to 6.02 (4.59 ± 0.01) kg. The absolute index of the bone component in 4-year-old girls has an individual variability of 3.04–5.17 (3.97 ± 0.01) kg, 5-year-old — 3.27–5.61 (4.20 ± 0.01) kg, 6-year-old — 3.34–5.77 (4.34 ± 0.01) kg and 7-year-old — 3.43–6.02 (4.40 ± 0.01) kg. Conclusions. The highest bone content was found in individuals with muscular somatotype, while the lowest values were in asthenoid and thoracic somatotypes for both males and females. Boys had higher bone component index at ages of 4 and 7.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.22141/2224-0551.18.6.2023.1626
M.L. Aryayev, L.I. Senkіvska, V.S. Biryukov, V.A. Pavlova, M.S. Streltsov, M.S. Streltsov, T.R. Kengelyan
Мета: покращити моніторинг орфанних та соціально значущих захворювань у дітей на основі використання географічної інформаційної системи (ГІС) і вивчити зв’язок між поширеністю дефіциту гормону росту (ДГР), муковісцидозу (МВ), гострого лімфобластного лейкозу (ГЛЛ), цукрового діабету 1-го типу (Т1ЦД) й еколого-геофізичними факторами навколишньої території. Матеріали та методи. Моніторинг випадків ДГР, МВ, ГЛЛ та Т1ЦД у дітей в Одеській області проводився з 2016 по 2020 роки. Ми зареєстрували дані 862 дітей, серед яких 92 мали ДГР, 54 — МВ, 88 — ГЛЛ і 628 — T1ЦД. У дослідженні використано клінічні та епідеміологічні методи, а також локальну ГІС. Для аналізу даних застосовували локальний медико-соціологічний шар ГІС і накладали його на еколого-геофізичний шар тієї ж ГІС. У дослідженні використано інформацію, отриману при проведенні Чорноморської геофізичної експедиції в Одеській області. Поширеність захворювань аналізували за χ2-тестом. Значення р < 0,05 вважалося статистично значущим. Результати. Під час перевірки «нульової гіпотези» щодо розподілу випадків ДГР, МВ, ГЛЛ та Т1ЦД у дітей за трьома фізико-географічними зонами Одеської області виявлено суттєві відмінності в поширеності захворювань на різних територіях. У лісостеповій зоні, зокрема в Ананьївському районі, виявлено найбільшу поширеність ДГР, водночас у Миколаївському районі степової зони переважав Т1ЦД. У Придністровській зоні у Біляївському районі найвищими були показники Т1ЦД, тоді як в Овідіопольському районі — показники МВ, а в Одесі — ГЛЛ. При дослідженні поширеності ДГР, МВ, ГЛЛ та Т1ЦД у дітей разом із картуванням геофізичних та екологічних аномалій в Одеській області виявлено істотну роль еколого-геофізичних факторів. Висновки. Використання методу ГІС при епідеміологічному дослідженні ДГР, МВ, ГЛЛ та Т1ЦД у дітей сприяє покращенню моніторингу орфанних та соціально значущих захворювань.
{"title":"Географічна інформаційна система в моніторингу орфанних та соціально значущих захворювань у дітей","authors":"M.L. Aryayev, L.I. Senkіvska, V.S. Biryukov, V.A. Pavlova, M.S. Streltsov, M.S. Streltsov, T.R. Kengelyan","doi":"10.22141/2224-0551.18.6.2023.1626","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1626","url":null,"abstract":"Мета: покращити моніторинг орфанних та соціально значущих захворювань у дітей на основі використання географічної інформаційної системи (ГІС) і вивчити зв’язок між поширеністю дефіциту гормону росту (ДГР), муковісцидозу (МВ), гострого лімфобластного лейкозу (ГЛЛ), цукрового діабету 1-го типу (Т1ЦД) й еколого-геофізичними факторами навколишньої території. Матеріали та методи. Моніторинг випадків ДГР, МВ, ГЛЛ та Т1ЦД у дітей в Одеській області проводився з 2016 по 2020 роки. Ми зареєстрували дані 862 дітей, серед яких 92 мали ДГР, 54 — МВ, 88 — ГЛЛ і 628 — T1ЦД. У дослідженні використано клінічні та епідеміологічні методи, а також локальну ГІС. Для аналізу даних застосовували локальний медико-соціологічний шар ГІС і накладали його на еколого-геофізичний шар тієї ж ГІС. У дослідженні використано інформацію, отриману при проведенні Чорноморської геофізичної експедиції в Одеській області. Поширеність захворювань аналізували за χ2-тестом. Значення р < 0,05 вважалося статистично значущим. Результати. Під час перевірки «нульової гіпотези» щодо розподілу випадків ДГР, МВ, ГЛЛ та Т1ЦД у дітей за трьома фізико-географічними зонами Одеської області виявлено суттєві відмінності в поширеності захворювань на різних територіях. У лісостеповій зоні, зокрема в Ананьївському районі, виявлено найбільшу поширеність ДГР, водночас у Миколаївському районі степової зони переважав Т1ЦД. У Придністровській зоні у Біляївському районі найвищими були показники Т1ЦД, тоді як в Овідіопольському районі — показники МВ, а в Одесі — ГЛЛ. При дослідженні поширеності ДГР, МВ, ГЛЛ та Т1ЦД у дітей разом із картуванням геофізичних та екологічних аномалій в Одеській області виявлено істотну роль еколого-геофізичних факторів. Висновки. Використання методу ГІС при епідеміологічному дослідженні ДГР, МВ, ГЛЛ та Т1ЦД у дітей сприяє покращенню моніторингу орфанних та соціально значущих захворювань.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.22141/2224-0551.18.6.2023.1631
V.O. Dytiatkovskyi, O.L. Krivusha, N.M. Tokareva
Background. Bronchial asthma (BA) in children is on one of the leading places in the morbidity and mortality structure among other allergic and atopic diseases. It can be developed in the form of a monoorganic phenotype (MOPh) or a polyorganic phenotype (POPh) with other nosologies of atopic march (AM): atopic dermatitis (AD) and allergic rhinitis/rhinoconjunctivitis (AR/ARC). This process is genetically determined, with single-nucleotide variants (SNV) of filaggrin (FLG), thymic stromal lymphopoietin (TSLP) and orsomucoid-like protein 3 (ORMDL3) genes playing a major role. The purpose of this study was to reveal the impact of rs_7927894 FLG, rs_11466749 TSLP and rs_7216389 ORMDL3 SNV genotype combinations in the development of MOPh and POPh of atopic BA in children. Materials and methods. One hundred and twenty-one children of the main group and 105 controls took part in the study. The criteria for inclusion into the main group were: age from 3 to 18 years, clinically established and laboratory confirmed diagnoses of MOPh BA, POPh BA + AR/ARC and AD + AR/ARC + BA. The criteria for inclusion in the control group were: age from 3 to 18 years, excluded diagnoses of BA, BA + AR/ARC and AD + AR/ARC + BA. All children underwent swabbing of the oral mucosa and real-time polymerase chain reaction with the obtained material to detect variants of rs_7927894 FLG, rs_11466749 TSLP and rs_7216389 ORMDL3 genotype combinations. The results were processed using the following statistical tools: logistic regression analysis with determination of odds ratio (OR) with 95% confidence interval (95% CI), receiver operating characteristic (ROC) analysis with determination of the area under the ROC curve (AUC), sensitivity (Se), specificity (Sp), Pearson’s correlation coefficient (r), Fisher’s exact test, Student’s t-test. The significance value was set at p < 0.05, trend to reliability — at p = 0.0–0.1. Results. The structure of the significantly most frequent genotypes in the cohorts of the main group was as follows: C/T rs_7927894 FLG + C/T rs_7216389 ORMDL3 — BA = 8.7 %; C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: BA = 21.7 %, BA + AR/ARC = 18.1 %, AD + AR/ARC + BA = 15.4 %; C/T rs_7927894 FLG + A/A rs_11466749 TSLP: BA + AR/ARC = 31.9 %, AD + AR/ARC + BA = 42.3 %. Next, indicators of the genotypic combinations impact on the risk of BA phenotypes development related to the control group are provided. MOPh BA: C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.299, OR = 9.44 (95% CI 2.07–43.03), AUC = 0.594 (0.507–0.682), Se/Sp = 21.7/97.1 % (p < 0.001). POPh BA + AR/ARC: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.136, OR = 1.88 (95% CI 0.94–3.74), AUC = 0.560 (0.493–0.626), Se/Sp 31.9/80.0 % (p = 0.071); C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.260, OR = 7.49 (95% CI 2.05–27.37), AUC = 0.576 (0.528–0.624), Se/Sp = 18.1/97.1 % (p < 0.001). POPh AD + AR/ARC + BA: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.207, OR = 2.93 (95% CI 1.18–7.31),
背景。儿童支气管哮喘(BA)在其他过敏性和特应性疾病的发病率和死亡率结构中处于领先地位。它可以以单有机表型(MOPh)或多有机表型(POPh)的形式发展，并伴有其他特应性进行性疾病(AM):特应性皮炎(AD)和过敏性鼻炎/鼻结膜炎(AR/ARC)。这一过程是由遗传决定的，聚丝蛋白(FLG)、胸腺基质淋巴生成素(TSLP)和orsomucloid like protein 3 - (ORMDL3)基因的单核苷酸变异(SNV)起主要作用。本研究旨在揭示rs_7927894 FLG、rs_11466749 TSLP和rs_7216389 ORMDL3 SNV基因型组合在特应性BA患儿MOPh和POPh发生中的影响。材料和方法。主组的121名儿童和对照组的105名儿童参加了这项研究。纳入主要组的标准为:年龄3 ~ 18岁，临床确诊和实验室确诊为MOPh BA、POPh BA + AR/ARC和AD + AR/ARC + BA。纳入对照组的标准为:年龄3 ~ 18岁，排除BA、BA + AR/ARC和AD + AR/ARC + BA诊断。所有患儿均采用口腔黏膜拭子，用获得的材料进行实时聚合酶链反应，检测rs_7927894 FLG、rs_11466749 TSLP和rs_7216389 ORMDL3基因型组合的变异。使用以下统计工具对结果进行处理:采用95%置信区间(95% CI)确定优势比(OR)的logistic回归分析，确定ROC曲线下面积(AUC)的受试者工作特征(ROC)分析，灵敏度(Se)，特异性(Sp)， Pearson相关系数(r)， Fisher精确检验，学生t检验。显著性值设为p <0.05，信度趋势- p = 0.0-0.1。结果。主组队列中显著频率最高的基因型结构为:C/T rs_7927894 FLG + C/T rs_7216389 - ormdl3 - BA = 8.7%;C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: BA = 21.7%， BA + AR/ARC = 18.1%， AD + AR/ARC + BA = 15.4%;C/T rs_7927894 FLG + A/A rs_11466749 TSLP: BA + AR/ARC = 31.9%， AD + AR/ARC + BA = 42.3%。接下来，我们提供了基因型组合对与对照组相关的BA表型发展风险的影响指标。MOPh BA: C/T rs_7927894 FLG + T/T rs_7216389 -ORMDL3: r = 0.299, OR = 9.44 (95% CI 2.07-43.03)， AUC = 0.594 (0.507-0.682)， Se/Sp = 21.7/ 97.1% (p <0.001)。POPh BA + AR/ARC: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.136, OR = 1.88 (95% CI 0.94 ~ 3.74)， AUC = 0.560 (0.493 ~ 0.626)， Se/Sp 31.9/ 80.0% (p = 0.071);C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.260, OR = 7.49 (95% CI 2.05 ~ 27.37)， AUC = 0.576 (0.528 ~ 0.624)， Se/Sp = 18.1/ 97.1% (p <0.001)。POPh AD + AR/ARC + BA: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.207, OR = 2.93 (95% CI 1.18 ~ 7.31)， AUC为0.612 (0.507 ~ 0.716)，Se/Sp = 42.3/ 80.0% (r <0.05);C/T rs_7927894 FLG + C/T rs_7216389 ORMDL3: r = 0.173, OR = 2.50 (95% CI 0.99 ~ 6.30)， AUC = 0.592 (0.489 ~ 0.695)， Se/Sp = 38.5/ 80.0% (p <0.05);C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.222, OR = 6.18 (95% CI 1.29-29.6)， AUC = 0.563 (0.490-0.635)， Se/Sp = 15.4/ 97.1% (p <0.01)。发生表型相关的关联比和风险:BA + AR/ARC与BA相关:C/T rs_7927894 FLG + C/T rs_7216389 ORMDL3: r = 0.171, OR = 3.50 (95% CI 0.75 ~ 16.41)， AUC = 0.582 (0.504 ~ 0.659)， Se/Sp = 25.0/ 91.3% (p = 0.095);AD + AR/ARC + BA与BA相关:C/T rs_7927894 FLG + C/T rs_7216389 -ORMDL3: r = 0.345, OR = 6.56 (95% CI 1.26-34.23)， AUC = 0.649 (0.537-0.761)， Se/Sp = 38.5/ 91.3% (r <0.05);C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.270, OR = 3.48 (95% CI 0.92 ~ 13.17)， AUC = 0.625 (0.500 ~ 0.750)， Se/Sp = 42.3/ 82.6% (p = 0.059)。结论。MOPh BA与SNV基因型组合C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3有显著关联，且发展风险增加。POPh BA + AR/ARC与C/T rs_7927894 FLG + A/A rs_11466749 TSLP和C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3的SNV基因型组合有显著相关性，且发展风险增加。POPh AD + AR/ARC + BA在以下SNV基因型组合中具有最大的相关性和增加的发展风险:C/T rs_7927894 FLG + A/A rs_11466749 TSLP, C/T rs_7927894 FLG + C/T rs_7216389 ORMDL3, C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3。
{"title":"Development of monoorganic and polyorganic phenotypes of bronchial asthma in children: the role of combined single-nucleotide variants","authors":"V.O. Dytiatkovskyi, O.L. Krivusha, N.M. Tokareva","doi":"10.22141/2224-0551.18.6.2023.1631","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1631","url":null,"abstract":"Background. Bronchial asthma (BA) in children is on one of the leading places in the morbidity and mortality structure among other allergic and atopic diseases. It can be developed in the form of a monoorganic phenotype (MOPh) or a polyorganic phenotype (POPh) with other nosologies of atopic march (AM): atopic dermatitis (AD) and allergic rhinitis/rhinoconjunctivitis (AR/ARC). This process is genetically determined, with single-nucleotide variants (SNV) of filaggrin (FLG), thymic stromal lymphopoietin (TSLP) and orsomucoid-like protein 3 (ORMDL3) genes playing a major role. The purpose of this study was to reveal the impact of rs_7927894 FLG, rs_11466749 TSLP and rs_7216389 ORMDL3 SNV genotype combinations in the development of MOPh and POPh of atopic BA in children. Materials and methods. One hundred and twenty-one children of the main group and 105 controls took part in the study. The criteria for inclusion into the main group were: age from 3 to 18 years, clinically established and laboratory confirmed diagnoses of MOPh BA, POPh BA + AR/ARC and AD + AR/ARC + BA. The criteria for inclusion in the control group were: age from 3 to 18 years, excluded diagnoses of BA, BA + AR/ARC and AD + AR/ARC + BA. All children underwent swabbing of the oral mucosa and real-time polymerase chain reaction with the obtained material to detect variants of rs_7927894 FLG, rs_11466749 TSLP and rs_7216389 ORMDL3 genotype combinations. The results were processed using the following statistical tools: logistic regression analysis with determination of odds ratio (OR) with 95% confidence interval (95% CI), receiver operating characteristic (ROC) analysis with determination of the area under the ROC curve (AUC), sensitivity (Se), specificity (Sp), Pearson’s correlation coefficient (r), Fisher’s exact test, Student’s t-test. The significance value was set at p < 0.05, trend to reliability — at p = 0.0–0.1. Results. The structure of the significantly most frequent genotypes in the cohorts of the main group was as follows: C/T rs_7927894 FLG + C/T rs_7216389 ORMDL3 — BA = 8.7 %; C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: BA = 21.7 %, BA + AR/ARC = 18.1 %, AD + AR/ARC + BA = 15.4 %; C/T rs_7927894 FLG + A/A rs_11466749 TSLP: BA + AR/ARC = 31.9 %, AD + AR/ARC + BA = 42.3 %. Next, indicators of the genotypic combinations impact on the risk of BA phenotypes development related to the control group are provided. MOPh BA: C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.299, OR = 9.44 (95% CI 2.07–43.03), AUC = 0.594 (0.507–0.682), Se/Sp = 21.7/97.1 % (p < 0.001). POPh BA + AR/ARC: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.136, OR = 1.88 (95% CI 0.94–3.74), AUC = 0.560 (0.493–0.626), Se/Sp 31.9/80.0 % (p = 0.071); C/T rs_7927894 FLG + T/T rs_7216389 ORMDL3: r = 0.260, OR = 7.49 (95% CI 2.05–27.37), AUC = 0.576 (0.528–0.624), Se/Sp = 18.1/97.1 % (p < 0.001). POPh AD + AR/ARC + BA: C/T rs_7927894 FLG + A/A rs_11466749 TSLP: r = 0.207, OR = 2.93 (95% CI 1.18–7.31), ","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"83 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.22141/2224-0551.18.6.2023.1637
T.O. Kryuchko, S.M. Tanianska
Diseases of cardiovascular system in children present an extremely urgent problem nowadays. According to the latest epidemiological studies, there has been an increase by almost 3.2 % in congenital heart disease in Ukrainian children over the past 20–30 years. Left ventricular non-compaction belongs to a group of unclassified cardiomyopathies with undetermined prevalence. At the same time, based on literature, this pathology is characterized by a late diagnosis and very high mortality. In the pathogenesis of the disease, structural changes in the myocardium occur during the embryonic stage, leading to its abnormal development. Left ventricular non-compaction is considered a genetically heterogenous disease, which is inherited in an autosomal dominant pattern. The article deals with a clinical case of diagnosis and management of a child with left ventricular non-compaction, which demonstrates to general practitioners, pediatricians, and pediatric cardiologists the algorithm for diagnosing and managing patients with a rare disease in order to make earlier diagnosis, prevent complications, and preserve patient’s life. The patient who has been suffered from pneumonia, complained of breathlessness and rapid fatigue during minor physical exertion was timely referred by the family doctor for consultation with a pediatric cardiologist. Laboratory and instrumental studies allowed to suspect a rare congenital heart disease — left ventricular non-compaction. Subsequently, the patient was regularly examined by cardiologists and cardiac surgeons, received protocol therapy, was registered in a state transplant registry, which allowed to wait for a donor with subsequent successful heart transplant surgery.
{"title":"Clinical case of diagnosis of left ventricular non-compaction in a child","authors":"T.O. Kryuchko, S.M. Tanianska","doi":"10.22141/2224-0551.18.6.2023.1637","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1637","url":null,"abstract":"Diseases of cardiovascular system in children present an extremely urgent problem nowadays. According to the latest epidemiological studies, there has been an increase by almost 3.2 % in congenital heart disease in Ukrainian children over the past 20–30 years. Left ventricular non-compaction belongs to a group of unclassified cardiomyopathies with undetermined prevalence. At the same time, based on literature, this pathology is characterized by a late diagnosis and very high mortality. In the pathogenesis of the disease, structural changes in the myocardium occur during the embryonic stage, leading to its abnormal development. Left ventricular non-compaction is considered a genetically heterogenous disease, which is inherited in an autosomal dominant pattern. The article deals with a clinical case of diagnosis and management of a child with left ventricular non-compaction, which demonstrates to general practitioners, pediatricians, and pediatric cardiologists the algorithm for diagnosing and managing patients with a rare disease in order to make earlier diagnosis, prevent complications, and preserve patient’s life. The patient who has been suffered from pneumonia, complained of breathlessness and rapid fatigue during minor physical exertion was timely referred by the family doctor for consultation with a pediatric cardiologist. Laboratory and instrumental studies allowed to suspect a rare congenital heart disease — left ventricular non-compaction. Subsequently, the patient was regularly examined by cardiologists and cardiac surgeons, received protocol therapy, was registered in a state transplant registry, which allowed to wait for a donor with subsequent successful heart transplant surgery.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.22141/2224-0551.18.6.2023.1628
Farhad Shaker Armishty, Ronahi Younis Ibrahim, Adam J. Adam, Racheen S. Haji, Zeen Khalil Ghazi, Parwar I. Tahir
Background. Healthcare systems are very concerned about childhood obesity, which continues to be a barrier to medical innovation. Over the last three decades, the frequency of childhood overweight/ obesity has increased significantly. Children spend a significant amount of time in front of electronic devices such as cell phones, games, computers, and television that is linked to a decreased physical activity and weight gain. The purpose of the study was to find the association between watching screen media and obesity in school-aged children from Zakho. Materials and methods. This is a cross-sectional, case-control, face-to-face survey study. All students aged 9–15 years from different schools were invited to complete the questionnaire. Well-trained medical students collected the anthropometric measurements of weight and height. Results. A total of 500 students between 9 and 15 years were interviewed. Approximately 72 % of them were males and 28 % were females. There was a significant association between a history of eating while watching screens and obesity (P = 0.003), as well as between the average number of hours spent daily using a computer and obesity (P = 0.016). Conclusions. In summary, this study supported the link between screen usage and childhood obesity in school-aged children. As a result, it is vital to create health promotion initiatives that emphasize a healthy 24-hour period, which includes a balanced amount of time spent engaging in physical activity, watching television, and sleeping.
{"title":"Obesity among school-age children from Zakho (Kurdistan, Iraq) is linked to viewing screen media","authors":"Farhad Shaker Armishty, Ronahi Younis Ibrahim, Adam J. Adam, Racheen S. Haji, Zeen Khalil Ghazi, Parwar I. Tahir","doi":"10.22141/2224-0551.18.6.2023.1628","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1628","url":null,"abstract":"Background. Healthcare systems are very concerned about childhood obesity, which continues to be a barrier to medical innovation. Over the last three decades, the frequency of childhood overweight/ obesity has increased significantly. Children spend a significant amount of time in front of electronic devices such as cell phones, games, computers, and television that is linked to a decreased physical activity and weight gain. The purpose of the study was to find the association between watching screen media and obesity in school-aged children from Zakho. Materials and methods. This is a cross-sectional, case-control, face-to-face survey study. All students aged 9–15 years from different schools were invited to complete the questionnaire. Well-trained medical students collected the anthropometric measurements of weight and height. Results. A total of 500 students between 9 and 15 years were interviewed. Approximately 72 % of them were males and 28 % were females. There was a significant association between a history of eating while watching screens and obesity (P = 0.003), as well as between the average number of hours spent daily using a computer and obesity (P = 0.016). Conclusions. In summary, this study supported the link between screen usage and childhood obesity in school-aged children. As a result, it is vital to create health promotion initiatives that emphasize a healthy 24-hour period, which includes a balanced amount of time spent engaging in physical activity, watching television, and sleeping.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"88 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.22141/2224-0551.18.6.2023.1636
Ola Hassouneh, Haytham Al-Oran, Eman Al-Smadi, Eman Qzih
Background. This review aims to discuss the definition of preoperative fasting in terms of gastric emptying in children prepared for surgery, to discuss the conflict between preoperative fasting duration and volume, and to explain the benefits and hazards associated with fasting which may contribute to physical distress or well-being. Materials and methods. A search in CINAHL, EBSCO, Science Direct, Google Scholar, PubMed, and the MЕDLINE database was conducted. The search generated 150 articles, 17 articles were included in this review. Results. The review revealed that preoperative fasting is one of the safe measures that should be concerned by all health care providers, at the same time focusing on the comprehensive assessment before surgery which helps in the prediction of hazards that may expose children to dangers. Conclusions. A major conflict is to have a policy for preoperative fasting, so new guidelines for children who undergo surgery should be applied and implemented.
{"title":"Preoperative fasting among children who undergoing surgery","authors":"Ola Hassouneh, Haytham Al-Oran, Eman Al-Smadi, Eman Qzih","doi":"10.22141/2224-0551.18.6.2023.1636","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1636","url":null,"abstract":"Background. This review aims to discuss the definition of preoperative fasting in terms of gastric emptying in children prepared for surgery, to discuss the conflict between preoperative fasting duration and volume, and to explain the benefits and hazards associated with fasting which may contribute to physical distress or well-being. Materials and methods. A search in CINAHL, EBSCO, Science Direct, Google Scholar, PubMed, and the MЕDLINE database was conducted. The search generated 150 articles, 17 articles were included in this review. Results. The review revealed that preoperative fasting is one of the safe measures that should be concerned by all health care providers, at the same time focusing on the comprehensive assessment before surgery which helps in the prediction of hazards that may expose children to dangers. Conclusions. A major conflict is to have a policy for preoperative fasting, so new guidelines for children who undergo surgery should be applied and implemented.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"87 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cat scratch disease (CSD), a zoonotic infection caused mainly by the bacterium Bartonella henselae (B.henselae), is widespread throughout the world. The main carriers of B.henselae are domestic cats that get infected by fleas. In humans, the disease occurs after a contact with a cat, typically through a bite or scratch from an infected animal. Difficulties in diagnosing the disease are associated with the variety of its clinical manifestations. Three clinical cases of CSD in children are presented, in two of them skin manifestations preceded common clinical symptoms such as fever and lymphadenopathy. In these patients, false diagnoses of scarlet fever and pityriasis rosea made it difficult to diagnose CSD in a timely manner. As a result, etiotropic treatment was started on days 16 and 21 of the illness, respectively. In the third case, a patient experienced abscess formation in one of the affected lymph nodes after prolonged fever for a month and regional lymphadenopathy, which necessitated surgical intervention. In all three cases, the family physicians did not adequately consider the epidemiological history, specifically failing to recognize the persistent, long-term contact of the children with cats. Patients received antibacterial therapy with clarithromycin, which led to clear positive dynamics and recovery. These clinical cases emphasize the importance of considering B.henselae infection in all cases of lymphadenopathy accompanied by skin rashes to ensure early diagnosis and appropriate treatment, thereby preventing complications, particularly when children have contact with cats.
{"title":"Difficulties in diagnosing cat scratch disease in children","authors":"O.V. Prokopiv, S.A. Lyshenyuk, V.S. Zhukovskyi, H.M. Karmazyn","doi":"10.22141/2224-0551.18.6.2023.1638","DOIUrl":"https://doi.org/10.22141/2224-0551.18.6.2023.1638","url":null,"abstract":"Cat scratch disease (CSD), a zoonotic infection caused mainly by the bacterium Bartonella henselae (B.henselae), is widespread throughout the world. The main carriers of B.henselae are domestic cats that get infected by fleas. In humans, the disease occurs after a contact with a cat, typically through a bite or scratch from an infected animal. Difficulties in diagnosing the disease are associated with the variety of its clinical manifestations. Three clinical cases of CSD in children are presented, in two of them skin manifestations preceded common clinical symptoms such as fever and lymphadenopathy. In these patients, false diagnoses of scarlet fever and pityriasis rosea made it difficult to diagnose CSD in a timely manner. As a result, etiotropic treatment was started on days 16 and 21 of the illness, respectively. In the third case, a patient experienced abscess formation in one of the affected lymph nodes after prolonged fever for a month and regional lymphadenopathy, which necessitated surgical intervention. In all three cases, the family physicians did not adequately consider the epidemiological history, specifically failing to recognize the persistent, long-term contact of the children with cats. Patients received antibacterial therapy with clarithromycin, which led to clear positive dynamics and recovery. These clinical cases emphasize the importance of considering B.henselae infection in all cases of lymphadenopathy accompanied by skin rashes to ensure early diagnosis and appropriate treatment, thereby preventing complications, particularly when children have contact with cats.","PeriodicalId":338009,"journal":{"name":"CHILD`S HEALTH","volume":"84 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：