Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001094
Ivan Voloshyn-Gaponov
The aim – to study the effectiveness of zinc salts in the treatment of patients with neurological forms of Wilson's disease (WD). Materials and methods. The analysis of the treatment results of 128 patients with hepatocerebral degeneration (71 men and 57 women) in the State Institution “Institute of Neurology, Psychiatry and Narcology of the National Academy of Medical Sciences of Ukraine” was carried out. At the time of hospitalization, the age of patients ranged from 5 to 55 years, an average of 27.3 years, and at the time of debut of the disease - from 1 year to 40 years, an average of 21.3 years. 33 patients underwent monotherapy with zinc salts, 63 – combined therapy with small doses of penicillamine and zinc salts, 32 – monotherapy with penicillamine. Results. Because of the treatment, 67.1 % of patients showed an improvement in neuropsychiatric status: speech improved significantly, tremor of the extremities and the amplitude of hyperkinesis decreased, muscle tone decreased, and cognitive functions improved. According to the international two-level rating scale (UWDRS), the total pathology index decreased by 21 points. Zinc salts are effective and low-toxic and can be the drug of choice in the treatment of patients with hepatocerebral degeneration in the pre-symptomatic stage of the disease, as well as at the stage of maintenance treatment, both as monotherapy and in combination with penicillamine. However, zinc salts and penicillamine are not enough for the treatment and rehabilitation of patients with hepatocerebral degeneration. Therefore, taking into account the clinical picture and the data of additional research methods, it is necessary to conduct courses of symptomatic treatment at least 1-2 times a year. Conclusions. Thus, it can be noted that zinc salts are very effective and low toxic, and, therefore, can be the drug of choice in the treatment of patients with HCD in the pre-symptomatic stage of the disease, as well as at the stage of maintenance therapy as a single drug, and in combination with penicillamine or other chelate drugs.
{"title":"MODERN ASPECTS OF PATHOGENETIC TREATMENT WITH ZINC SALTS OF PATIENTS WITH WILSON'S DISEASE IN UKRAINE","authors":"Ivan Voloshyn-Gaponov","doi":"10.21303/2585-663.2019.001094","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001094","url":null,"abstract":"The aim – to study the effectiveness of zinc salts in the treatment of patients with neurological forms of Wilson's disease (WD). Materials and methods. The analysis of the treatment results of 128 patients with hepatocerebral degeneration (71 men and 57 women) in the State Institution “Institute of Neurology, Psychiatry and Narcology of the National Academy of Medical Sciences of Ukraine” was carried out. At the time of hospitalization, the age of patients ranged from 5 to 55 years, an average of 27.3 years, and at the time of debut of the disease - from 1 year to 40 years, an average of 21.3 years. 33 patients underwent monotherapy with zinc salts, 63 – combined therapy with small doses of penicillamine and zinc salts, 32 – monotherapy with penicillamine. Results. Because of the treatment, 67.1 % of patients showed an improvement in neuropsychiatric status: speech improved significantly, tremor of the extremities and the amplitude of hyperkinesis decreased, muscle tone decreased, and cognitive functions improved. According to the international two-level rating scale (UWDRS), the total pathology index decreased by 21 points. Zinc salts are effective and low-toxic and can be the drug of choice in the treatment of patients with hepatocerebral degeneration in the pre-symptomatic stage of the disease, as well as at the stage of maintenance treatment, both as monotherapy and in combination with penicillamine. However, zinc salts and penicillamine are not enough for the treatment and rehabilitation of patients with hepatocerebral degeneration. Therefore, taking into account the clinical picture and the data of additional research methods, it is necessary to conduct courses of symptomatic treatment at least 1-2 times a year. Conclusions. Thus, it can be noted that zinc salts are very effective and low toxic, and, therefore, can be the drug of choice in the treatment of patients with HCD in the pre-symptomatic stage of the disease, as well as at the stage of maintenance therapy as a single drug, and in combination with penicillamine or other chelate drugs.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89854333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001097
O. Samoilenko
Aim: to evaluate the effectiveness of preventing the formation of microbial biofilms on the surfaces of implants made of titanium alloys VT5-1, VT6 with a transformed surface into anatase. Materials and methods. The study was carried out in the bacteriological laboratory of the Severodonetsk multidisciplinary city hospital from January 2019 to September 2019. The ability to prevent the formation of microbial biofilms of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated. The static cell adhesion method and the disco diffuse method were used. The surface of the plates was examined on a XS 6220 UCMJS05100KPA microscope. The intensity of the formation of microbial biofilms on the plates was evaluated visually by the four-cross system. Results. On all coatings with anatase, in comparison with unmodified plates, the intensity of initial adhesion and the number of bacteria fixed on the surface of the colonies markedly decreased. On surfaces with silica crystals, biofilm formation was minimal. The altered surface of titanium alloys VT5-1 and VT6 anatase and anatase with silicon dioxide crystals does not inhibit microflora growth. Conclusions. The transformed surface of titanium implants into anatase and anatase with silicon dioxide crystals prevents the fixation of biofilms of microbial associations.
{"title":"RESEARCH OF ANTIBACTERIAL PROPERTIES OF THE SURFACE OF THE TITANIUM ALLOYS VT5-1 AND VT6 CONVERTED TO ANATASE TO PREVENT BACTERIAL ADHESION","authors":"O. Samoilenko","doi":"10.21303/2585-663.2019.001097","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001097","url":null,"abstract":"Aim: to evaluate the effectiveness of preventing the formation of microbial biofilms on the surfaces of implants made of titanium alloys VT5-1, VT6 with a transformed surface into anatase. Materials and methods. The study was carried out in the bacteriological laboratory of the Severodonetsk multidisciplinary city hospital from January 2019 to September 2019. The ability to prevent the formation of microbial biofilms of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated. The static cell adhesion method and the disco diffuse method were used. The surface of the plates was examined on a XS 6220 UCMJS05100KPA microscope. The intensity of the formation of microbial biofilms on the plates was evaluated visually by the four-cross system. Results. On all coatings with anatase, in comparison with unmodified plates, the intensity of initial adhesion and the number of bacteria fixed on the surface of the colonies markedly decreased. On surfaces with silica crystals, biofilm formation was minimal. The altered surface of titanium alloys VT5-1 and VT6 anatase and anatase with silicon dioxide crystals does not inhibit microflora growth. Conclusions. The transformed surface of titanium implants into anatase and anatase with silicon dioxide crystals prevents the fixation of biofilms of microbial associations.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88730014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001093
M. Kuznetsova
The aim of this study was to determine the influence of rats-mothers food ration with nutrient deficiency on the morphology and functional status of the liver of their two months offspring. Materials and methods. The experiments were performed on the offspring of WAG population rats, which was prenatal under nutritional deficiency. The offspring of rats (26 specimens) were divided into two groups, 50 % of the rats constituted the control group (i.e., their mothers received the vivarium baseline diet), the other 13 animals were in the 2nd (main) group. The young rats were removed from the experiment two months after birth by decapitation. Morpho-functional condition of the liver of the offspring rats treated with pregnancy deficiency of fat and carbohydrate diet was evaluated according to the results of immunohistochemical study of markers of liver vascular endothelium damage - endothelial synthase of nitric oxide (eNOS) and inducible nitric oxide synthase (iNOS) and biochemical study of liver tissue homogenates (studied fractional composition of lipids and organ glycogen). Results. It was found that the nutritional deficiency of rats-mothers had a significant effect on the structural and functional state of the liver of their bi-monthly offspring, which was manifested by a decrease in the level of endothelial synthase expression, which indicated a high degree of damage to the liver endothelium. Biochemical study of liver homogenates revealed signs of impaired secretion and reuptake of lipids, which were manifested by an increase in CL, TG and EFAs accumulation, as well as a decrease in liver PL and glycogen, which indicated the presence of pronounced disorders of carbohydrate and lipid metabolism. Conclusions. The obtained data allow us to establish the negative nature of the effect of deficiency of fats and carbohydrates in the diet of rats-mothers on the structural and functional state of the liver of their bi-monthly offspring. This condition was accompanied, both by signs of liver vascular endothelial damage and metabolic disorders, this all suggests that the identified disorders may be a risk factor for fatty hepatosis as well as liver cirrhosis in the future.
{"title":"THE INFLUENCE OF RATS-MOTHERS FOOD RATION WITH NUTRIENT DEFICIENCY ON THE STRUCTURAL-FUNCTIONAL STATE OF THE LIVER OF THEIR TWO MONTHS OFFSPRING","authors":"M. Kuznetsova","doi":"10.21303/2585-663.2019.001093","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001093","url":null,"abstract":"The aim of this study was to determine the influence of rats-mothers food ration with nutrient deficiency on the morphology and functional status of the liver of their two months offspring. Materials and methods. The experiments were performed on the offspring of WAG population rats, which was prenatal under nutritional deficiency. The offspring of rats (26 specimens) were divided into two groups, 50 % of the rats constituted the control group (i.e., their mothers received the vivarium baseline diet), the other 13 animals were in the 2nd (main) group. The young rats were removed from the experiment two months after birth by decapitation. Morpho-functional condition of the liver of the offspring rats treated with pregnancy deficiency of fat and carbohydrate diet was evaluated according to the results of immunohistochemical study of markers of liver vascular endothelium damage - endothelial synthase of nitric oxide (eNOS) and inducible nitric oxide synthase (iNOS) and biochemical study of liver tissue homogenates (studied fractional composition of lipids and organ glycogen). Results. It was found that the nutritional deficiency of rats-mothers had a significant effect on the structural and functional state of the liver of their bi-monthly offspring, which was manifested by a decrease in the level of endothelial synthase expression, which indicated a high degree of damage to the liver endothelium. Biochemical study of liver homogenates revealed signs of impaired secretion and reuptake of lipids, which were manifested by an increase in CL, TG and EFAs accumulation, as well as a decrease in liver PL and glycogen, which indicated the presence of pronounced disorders of carbohydrate and lipid metabolism. Conclusions. The obtained data allow us to establish the negative nature of the effect of deficiency of fats and carbohydrates in the diet of rats-mothers on the structural and functional state of the liver of their bi-monthly offspring. This condition was accompanied, both by signs of liver vascular endothelial damage and metabolic disorders, this all suggests that the identified disorders may be a risk factor for fatty hepatosis as well as liver cirrhosis in the future.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86977834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001089
L. Oreshko, E. Semenova, A. Shomin, Stanislav Sitkin
Aim: to investigate a fecal microbiota composition and to identify candidate biomarkers of celiac disease (CD) by serum metabolomics analysis. Methods: the quantitative real-time polymerase chain reaction was used for fecal microbiota assessment. Serum metabolomic assays were conducted using the GC–MS. Results: serum of CD patients showed significant increases in stearic acid, 2-HIVA, succinate, fumarate and benzoate compared to HC. A decrease in the level of eicosadiene and an increase in AA in blood were determined. The ratio of AA to EDA was statistically significant (4.84 vs. 3.28, p=0.033). The elongase activity index in patients with celiac disease tended to increase (p=0.067). The colon microbiome in CD was characterized by decreasing in the level of butyrate-producing Faecalibacterium prausnitzii (F.p.) and Bifidobacterium spp.. Significant negative correlations were observed; between the levels of Bifidobacterium spp. and F.p. and the concentration of succinic acid (rs=–0.343 [p=0.026] and rs=–0.430 [p=0.005], respectively); the F.p. and the fumaric acid (r=–0.429, p=0.005); the benzoic acid and the amount of Bifidobacterium spp. (r=–0.341, p=0.025). Conclusion: significant changes in serum levels of microbial and endogenous metabolites, reflecting some metabolic pathways disturbances were observed in CD. Metabolites and metabolomic index reflecting the balance between pro-inflammatory and anti-inflammatory components, may be considered as candidate biomarkers of chronic inflammation and metabolic dysbiosis in CD. An increased B. fragilis/F.p. ratio can serve as available biomarker for intestinal pro-inflammatory dysbiosis in CD.
{"title":"VIOLATION OF MICROBIAL AND ENDOGENOUS METABOLISM IN CELIAC DISEASE","authors":"L. Oreshko, E. Semenova, A. Shomin, Stanislav Sitkin","doi":"10.21303/2585-663.2019.001089","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001089","url":null,"abstract":"Aim: to investigate a fecal microbiota composition and to identify candidate biomarkers of celiac disease (CD) by serum metabolomics analysis. Methods: the quantitative real-time polymerase chain reaction was used for fecal microbiota assessment. Serum metabolomic assays were conducted using the GC–MS. Results: serum of CD patients showed significant increases in stearic acid, 2-HIVA, succinate, fumarate and benzoate compared to HC. A decrease in the level of eicosadiene and an increase in AA in blood were determined. The ratio of AA to EDA was statistically significant (4.84 vs. 3.28, p=0.033). The elongase activity index in patients with celiac disease tended to increase (p=0.067). The colon microbiome in CD was characterized by decreasing in the level of butyrate-producing Faecalibacterium prausnitzii (F.p.) and Bifidobacterium spp.. Significant negative correlations were observed; between the levels of Bifidobacterium spp. and F.p. and the concentration of succinic acid (rs=–0.343 [p=0.026] and rs=–0.430 [p=0.005], respectively); the F.p. and the fumaric acid (r=–0.429, p=0.005); the benzoic acid and the amount of Bifidobacterium spp. (r=–0.341, p=0.025). Conclusion: significant changes in serum levels of microbial and endogenous metabolites, reflecting some metabolic pathways disturbances were observed in CD. Metabolites and metabolomic index reflecting the balance between pro-inflammatory and anti-inflammatory components, may be considered as candidate biomarkers of chronic inflammation and metabolic dysbiosis in CD. An increased B. fragilis/F.p. ratio can serve as available biomarker for intestinal pro-inflammatory dysbiosis in CD.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83316608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001092
A. Gavryluk, V. Voronov, R. Zharlinska
Expert diagnostics of forensic causal determinations should be based on a dialectical-materialistic approach, provided with a modern level of specially applied theoretical knowledge of pathology, etiology and pathogenesis of traumatic processes, as well as stimulated by urgent requests of the theory and practice of forensic medical expertise (FME). The aim of this work is to determine causal estimates of the components of forensic objects: natural and causal – through retrospective causal modelling. Materials and methods. The research material was archival documents of the Vinnytsia Regional Bureau of Forensics (Ukraine) for 2009–2012 with cases of violent death from injuries. 27 deterministic models were constructed by modelling the regular relationships in traumatic processes. Result. The qualitative unity of the forensic object is simulated: empirical (natural) and causal (determinant). The existence of an object determinant in concordance of the determinant object with the deterministic object is stated. The method of investigation of the determinants of expertise was proposed – logical retrospective modelling of object determinants dependencies. An assumed model of the dependency model is a multi-link time-chain of forensic medical determination. The adequacy of the proposed method of logical modelling of determinants in forensic examination is confirmed with the help of known scientific knowledge about dual, causal determination, causal diagnostics of determinants and forensic cause and effect relationships. It is proved that forensic expertise is a multidisciplinary branch of science and reflects the realized integration of different scientific knowledge. However, diagnosis of "causes of violent and non-violent death" requires the development of an applied methodology for FME from the standpoint of modern philosophical concepts of causality and general theory of systems, the achievements of which must be used in the construction of the methodology of FME. Conclusions. From the results of this study, it follows that causation is only a moment of determination, and purely causal modelling as an exploration of a particular type of determination cannot give a complete explanation for the deterministic relationship. The property of necessity of a specific reason determines the tendency, the orientation of development in the form of possibility, which is actualized only by the complete set of determinants - causal and noncausal, conditional.
{"title":"CAUSAL EVALUATIONS OF THE FORENSIC OBJECTS IN THE TRAUMATIC PROCESS","authors":"A. Gavryluk, V. Voronov, R. Zharlinska","doi":"10.21303/2585-663.2019.001092","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001092","url":null,"abstract":"Expert diagnostics of forensic causal determinations should be based on a dialectical-materialistic approach, provided with a modern level of specially applied theoretical knowledge of pathology, etiology and pathogenesis of traumatic processes, as well as stimulated by urgent requests of the theory and practice of forensic medical expertise (FME). The aim of this work is to determine causal estimates of the components of forensic objects: natural and causal – through retrospective causal modelling. Materials and methods. The research material was archival documents of the Vinnytsia Regional Bureau of Forensics (Ukraine) for 2009–2012 with cases of violent death from injuries. 27 deterministic models were constructed by modelling the regular relationships in traumatic processes. Result. The qualitative unity of the forensic object is simulated: empirical (natural) and causal (determinant). The existence of an object determinant in concordance of the determinant object with the deterministic object is stated. The method of investigation of the determinants of expertise was proposed – logical retrospective modelling of object determinants dependencies. An assumed model of the dependency model is a multi-link time-chain of forensic medical determination. The adequacy of the proposed method of logical modelling of determinants in forensic examination is confirmed with the help of known scientific knowledge about dual, causal determination, causal diagnostics of determinants and forensic cause and effect relationships. It is proved that forensic expertise is a multidisciplinary branch of science and reflects the realized integration of different scientific knowledge. However, diagnosis of \"causes of violent and non-violent death\" requires the development of an applied methodology for FME from the standpoint of modern philosophical concepts of causality and general theory of systems, the achievements of which must be used in the construction of the methodology of FME. Conclusions. From the results of this study, it follows that causation is only a moment of determination, and purely causal modelling as an exploration of a particular type of determination cannot give a complete explanation for the deterministic relationship. The property of necessity of a specific reason determines the tendency, the orientation of development in the form of possibility, which is actualized only by the complete set of determinants - causal and noncausal, conditional.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85582945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-09DOI: 10.21303/2585-663.2019.001096
I. Sakhanda, O. Orlov
The aim. To substantiate the effectiveness of the use of VAC-therapy in the treatment of wounds in patients with diabetic foot syndrome. Materials and methods. The patient with a complicated form of diabetic foot syndrome was examined. The monitoring of the course of the wound process is supplemented by clinical, cytological, microbiological and morphological criteria. In the treatment of patients we used vacuum therapy apparatus. Vacuum wound therapy was performed in negative pressure regimens in the range of 80–125 mm Hg. Results. In the pattern of morbidity in economically developed countries, diabetes mellitus (DM) occupies one of the first places. Its prevalence is 1.5–6 %. The number of patients with diabetes in Ukraine is likely to reach 14 million by 2020. Lesions of the lower extremities of different genesis occur in 30–80 % of people with impaired carbohydrate metabolism. Most often, these lesions are complicated by the development of chronic peptic ulcer defects, which, when untimely diagnosed and inadequate treatment, leads to amputation of the affected limb. Even when radical surgery is avoided, the long and costly treatment of trophic disorders of the soft tissues of the feet and legs leads to huge costs of both material resources and time for medical staff. In 15 % of patients with DM, trophic ulcers of the lower extremities are diagnosed. Recent studies have focused on such important aspects of the study of this issue as the early diagnosis of soft tissue lesions in individuals with DM, the development of treatment aimed at preventing amputations, facilitating the further rehabilitation of patients. Vacuum therapy is one of the new methods of treating wound defects, including in patients with diabetic foot syndrome. Topical application of vacuum therapy has already become an integral therapeutic option for wound debridement in patients with diabetic foot syndrome. However, when applying the classic vacuum therapy technique, these patients have the problem of delaying the outflow of tissue exudate and lysis detritus to the vacuum system. Conclusions. Optimization of the technique of the influence of negative pressure on the wound by applying forced drainage with irrigation of the wound with an antiseptic can maintain a constantly high concentration of antimicrobial agent in the wound, increase the speed of wound dialysis and prevent clogging of pores of the sponge and the bacterial colonies in it. The application of this technique avoids high amputation, accelerates the time of wound healing, and retains the supporting function of the lower extremity.
的目标。目的:探讨vaca治疗糖尿病足综合征患者伤口的有效性。材料和方法。对患有复杂形式糖尿病足综合征的患者进行了检查。对伤口过程的监测由临床、细胞学、微生物学和形态学标准补充。在对患者的治疗中，我们使用了真空治疗仪。负压方案在80-125 mm Hg范围内进行真空创面治疗。在经济发达国家的发病模式中，糖尿病(DM)占据了第一位。其患病率为1.5% - 6%。到2020年，乌克兰的糖尿病患者人数可能达到1400万。30 - 80%的碳水化合物代谢受损患者会出现不同原因的下肢病变。大多数情况下，这些病变会并发慢性消化性溃疡缺陷，如果诊断不及时，治疗不充分，会导致受影响肢体截肢。即使避免了根治性手术，对足部和腿部软组织营养性疾病的长期和昂贵的治疗也会给医务人员带来巨大的物质资源和时间成本。15%的糖尿病患者被诊断为下肢营养性溃疡。最近的研究集中在研究这一问题的重要方面，如糖尿病患者软组织病变的早期诊断，旨在预防截肢的治疗方法的发展，促进患者的进一步康复。真空疗法是治疗创面缺损的新方法之一，包括治疗糖尿病足综合征患者。局部应用真空疗法已经成为糖尿病足综合征患者伤口清创的一种整体治疗选择。然而，当应用经典的真空治疗技术时，这些患者存在组织渗出物和裂解碎屑向真空系统流出延迟的问题。结论。优化负压对创面影响的技术，采用强制引流配合消菌剂冲洗创面，可以保持创面中抗菌药物的持续高浓度，加快创面透析速度，防止海绵毛孔和菌落堵塞。该技术的应用避免了高位截肢，加快了创面愈合时间，保留了下肢的支撑功能。
{"title":"APPLICATION OF VAC-THERAPY IN THE TREATMENT OF DIABETIC FOOT","authors":"I. Sakhanda, O. Orlov","doi":"10.21303/2585-663.2019.001096","DOIUrl":"https://doi.org/10.21303/2585-663.2019.001096","url":null,"abstract":"The aim. To substantiate the effectiveness of the use of VAC-therapy in the treatment of wounds in patients with diabetic foot syndrome. Materials and methods. The patient with a complicated form of diabetic foot syndrome was examined. The monitoring of the course of the wound process is supplemented by clinical, cytological, microbiological and morphological criteria. In the treatment of patients we used vacuum therapy apparatus. Vacuum wound therapy was performed in negative pressure regimens in the range of 80–125 mm Hg. Results. In the pattern of morbidity in economically developed countries, diabetes mellitus (DM) occupies one of the first places. Its prevalence is 1.5–6 %. The number of patients with diabetes in Ukraine is likely to reach 14 million by 2020. Lesions of the lower extremities of different genesis occur in 30–80 % of people with impaired carbohydrate metabolism. Most often, these lesions are complicated by the development of chronic peptic ulcer defects, which, when untimely diagnosed and inadequate treatment, leads to amputation of the affected limb. Even when radical surgery is avoided, the long and costly treatment of trophic disorders of the soft tissues of the feet and legs leads to huge costs of both material resources and time for medical staff. In 15 % of patients with DM, trophic ulcers of the lower extremities are diagnosed. Recent studies have focused on such important aspects of the study of this issue as the early diagnosis of soft tissue lesions in individuals with DM, the development of treatment aimed at preventing amputations, facilitating the further rehabilitation of patients. Vacuum therapy is one of the new methods of treating wound defects, including in patients with diabetic foot syndrome. Topical application of vacuum therapy has already become an integral therapeutic option for wound debridement in patients with diabetic foot syndrome. However, when applying the classic vacuum therapy technique, these patients have the problem of delaying the outflow of tissue exudate and lysis detritus to the vacuum system. Conclusions. Optimization of the technique of the influence of negative pressure on the wound by applying forced drainage with irrigation of the wound with an antiseptic can maintain a constantly high concentration of antimicrobial agent in the wound, increase the speed of wound dialysis and prevent clogging of pores of the sponge and the bacterial colonies in it. The application of this technique avoids high amputation, accelerates the time of wound healing, and retains the supporting function of the lower extremity.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84095552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00765
S. Omel'chuk, A. Syrota
Some studies have proven possible potentiating toxic effect of combined pesticides. The potentiating effect may be related to the impact of a certain substance on enzymatic activity of another one. Due to combined preparations popularity, the importance of evaluating probability of summation and synergetic or antagonizing action of the one-purpose substances becomes particularly important. Aim of the research is to study peculiarities of histomorphological liver changes associated with combined effect of active substances with one-purpose action. Methods. The authors have used toxicological, histochemical and statistical study methods. 45 rats have been used for the study. The liver specimens for histochemical analysis were selected immediately after dissection of the rats, from which the 5 micrometer sections were prepared, using freezing microtome. After leaving the sections in cold acetone, the histochemical reaction on determining the γ-glutamyl transpeptidase, a transformed hepatocyte marker, which forms nodules while proliferates, was conducted. Results. The authors have established that the above-mentioned characteristics in animals which were administered the drug (benthiavalicarb-isopropyl+folpet) do not differ from negative control group. General specific area of nodules per сm2 and specific quantity of nodules per сm2 haven’t reliably changed, the control group exceeding these data by 18 % and 12 %, respectively. Discussion. The study of the benthiavalicarb-isopropyl and folpet mixture effect on animals has not revealed liver tissue proliferation of the carcinogen-transformed cells and appearance of hyperplastic nodes, which express the γ-glutamyl transpeptidase, a histochemical marker of pre-tumor changes. So, the fungicide (benthiavalicarb-isopropyl+folpet) has not induced multiplication or size increase of glutamyl transpeptidase nodules, which evidences about loss of the modifying effect of folpet on the benthiavalicarb-isopropyl carcinogenicity in this preparation formula. The obtained results may be used when deciding on administration of combined fungicides with active substances of one-purpose action.
{"title":"PECULIARITIES OF HISTOMORPHOLOGICAL LIVER CHANGES ASSOCIATED WITH COMBINED EFFECT OF ACTIVE SUBSTANCES WITH ONE-PURPOSE ACTION","authors":"S. Omel'chuk, A. Syrota","doi":"10.21303/2585-663.2018.00765","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00765","url":null,"abstract":"Some studies have proven possible potentiating toxic effect of combined pesticides. The potentiating effect may be related to the impact of a certain substance on enzymatic activity of another one. Due to combined preparations popularity, the importance of evaluating probability of summation and synergetic or antagonizing action of the one-purpose substances becomes particularly important. Aim of the research is to study peculiarities of histomorphological liver changes associated with combined effect of active substances with one-purpose action. Methods. The authors have used toxicological, histochemical and statistical study methods. 45 rats have been used for the study. The liver specimens for histochemical analysis were selected immediately after dissection of the rats, from which the 5 micrometer sections were prepared, using freezing microtome. After leaving the sections in cold acetone, the histochemical reaction on determining the γ-glutamyl transpeptidase, a transformed hepatocyte marker, which forms nodules while proliferates, was conducted. Results. The authors have established that the above-mentioned characteristics in animals which were administered the drug (benthiavalicarb-isopropyl+folpet) do not differ from negative control group. General specific area of nodules per сm2 and specific quantity of nodules per сm2 haven’t reliably changed, the control group exceeding these data by 18 % and 12 %, respectively. Discussion. The study of the benthiavalicarb-isopropyl and folpet mixture effect on animals has not revealed liver tissue proliferation of the carcinogen-transformed cells and appearance of hyperplastic nodes, which express the γ-glutamyl transpeptidase, a histochemical marker of pre-tumor changes. So, the fungicide (benthiavalicarb-isopropyl+folpet) has not induced multiplication or size increase of glutamyl transpeptidase nodules, which evidences about loss of the modifying effect of folpet on the benthiavalicarb-isopropyl carcinogenicity in this preparation formula. The obtained results may be used when deciding on administration of combined fungicides with active substances of one-purpose action.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73522207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00771
L. Demianenko
According to the consensus of An Endocrine Society Clinical Practice in Princeton, USA, worsening well-being and dystrophic mood, permanent weakness and altered sexual function, including libido decline and lack of orgasm, were considered as typical signs of androgen deficiency syndrome. Particular attention is required to study the state of the genitourinary system, which is hormone dependent, so it is obvious that age-associated symptoms of pathological processes of the lower urinary tract are likely to be considered as a "urological mask" of the deficiency of sex hormones. Aim of the work. Identify and describe the most common urogenital disorders in women of reproductive age with androgen deficiency. Materials and methods. The study was conducted at the Ukrainian Scientific and Practical Center of Endocrine Surgery, Endocrine organs and tissues transplantation of the Ministry of Health of Ukraine during 2017–2018 years. A survey was conducted on 80 women of reproductive age who had major complaints of sexual disturbances (decreased libido and lack of orgasm, as well as dyspareunia as the main manifestations of androgen deficiency in women) and 30 healthy reproductive women without complaints of sexual dysfunction. General clinical methods were used (questionnaire of patients with a detailed study of socio-economic status, somatic, gynecological, obstetric and sexual history). The condition of external and internal genital organs was evaluated in the study of cervix and vagina in mirrors and gynecological bimanual study. At the same time, batches of analyzes for bacteriological research and colpocytology studies were conducted. The “HAWK 2102 EXL” (Germany) apparatus was used for ultrasound examination of pelvic organs using transabdominal and transvaginal convection sensors with frequencies of 3.5 and 5 MHz, respectively. Consultation of the urologist in order to exclude organic urological pathology and, if necessary, cystoscopy was also done. In the plasma of venous blood, the following hormonal indices were also determined by solid phase IFA, namely, content: free testosterone, DHEA, androstenedione, prolactin, ACTH, cortisol, FSH, LH, estradiol, progesterone. Results. It was revealed after exclusion of the urologist of the organic pathology of the urinary tract, in 49 % (39) of the main group, there were disturbances of urination associated with atrophic changes in the distal sections of the urinary tract. The revealed signs of vulvovaginal atrophy: pH 6.1±0.7 in women of the main group and pH 4.3±0.5 in women of the control group, in addition reduction of the karyopyknotic index and increase of the ripening index as signs of atrophic changes in the vaginal mucosa and cervical canal, was detected in 70 % (56) women in the main group and not found in the control group. At the same time, the prolapse of the genitalia of mild degree in women of the main group was detected in 32 % (27), and in the control group, this pathology was not found. C
{"title":"UROGENITAL DISORDERS IN WOMEN OF REPRODUCTIVE AGE WITH ANDROGEN DEFICIENCY","authors":"L. Demianenko","doi":"10.21303/2585-663.2018.00771","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00771","url":null,"abstract":"According to the consensus of An Endocrine Society Clinical Practice in Princeton, USA, worsening well-being and dystrophic mood, permanent weakness and altered sexual function, including libido decline and lack of orgasm, were considered as typical signs of androgen deficiency syndrome. Particular attention is required to study the state of the genitourinary system, which is hormone dependent, so it is obvious that age-associated symptoms of pathological processes of the lower urinary tract are likely to be considered as a \"urological mask\" of the deficiency of sex hormones. Aim of the work. Identify and describe the most common urogenital disorders in women of reproductive age with androgen deficiency. Materials and methods. The study was conducted at the Ukrainian Scientific and Practical Center of Endocrine Surgery, Endocrine organs and tissues transplantation of the Ministry of Health of Ukraine during 2017–2018 years. A survey was conducted on 80 women of reproductive age who had major complaints of sexual disturbances (decreased libido and lack of orgasm, as well as dyspareunia as the main manifestations of androgen deficiency in women) and 30 healthy reproductive women without complaints of sexual dysfunction. General clinical methods were used (questionnaire of patients with a detailed study of socio-economic status, somatic, gynecological, obstetric and sexual history). The condition of external and internal genital organs was evaluated in the study of cervix and vagina in mirrors and gynecological bimanual study. At the same time, batches of analyzes for bacteriological research and colpocytology studies were conducted. The “HAWK 2102 EXL” (Germany) apparatus was used for ultrasound examination of pelvic organs using transabdominal and transvaginal convection sensors with frequencies of 3.5 and 5 MHz, respectively. Consultation of the urologist in order to exclude organic urological pathology and, if necessary, cystoscopy was also done. In the plasma of venous blood, the following hormonal indices were also determined by solid phase IFA, namely, content: free testosterone, DHEA, androstenedione, prolactin, ACTH, cortisol, FSH, LH, estradiol, progesterone. Results. It was revealed after exclusion of the urologist of the organic pathology of the urinary tract, in 49 % (39) of the main group, there were disturbances of urination associated with atrophic changes in the distal sections of the urinary tract. The revealed signs of vulvovaginal atrophy: pH 6.1±0.7 in women of the main group and pH 4.3±0.5 in women of the control group, in addition reduction of the karyopyknotic index and increase of the ripening index as signs of atrophic changes in the vaginal mucosa and cervical canal, was detected in 70 % (56) women in the main group and not found in the control group. At the same time, the prolapse of the genitalia of mild degree in women of the main group was detected in 32 % (27), and in the control group, this pathology was not found. C","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81625203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00773
L. Semeniuk
The article presents new data on the significance of vitamin D deficiency in the formation of endometrial structure disorders in women with luteal phase defect and unrupted follicle syndrome. Disorders of the menstrual cycle caused by a progesterone deficiency from the position of influence on the onset and progression of pregnancy are considered. Fertility of women, especially with endocrine imbalances, deserves special attention, since the frequency of early pregnancy loss in them is about 67 %. Aim: study of the effects of vitamin D deficiency and progesterone deficiency in the luteinized unruptured follicle syndrome (Geis-Genes) and the luteal phase defect on the reproductive potential of women. Materials and methods: a hormonal study, determination of vitamin D levels, a sonographic study conducted on days 5–7 and 21–24 of the menstrual cycle in the periconceptional supplementation program of 45 women with luteal phase deficiency (LPD) and luteinized unruptured follicle syndrome (LUF). Results: The relationship between the content of vitamin D and the hormonal levels of the reproductive system of women with LPD and LUF is traced. The role of reducing vitamin D levels in the formation of hormonal imbalances in women of reproductive age has been established. Conclusions: – progesterone deficiency entails changes in various regulatory substances of the reproductive system, and therefore the periconceptional supplementation must be individual. – The introduction of the definition of vitamin D in the periconceptional supplementation plan and its correction are justified and necessary to improve the structure of the endometrium and its secretory transformation in women with progesterone imbalance.
{"title":"PROGESTERONE INSUFFICIENCY AS A PREDICTOR OF FUTURE REPRODUCTIVE LOSSES","authors":"L. Semeniuk","doi":"10.21303/2585-663.2018.00773","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00773","url":null,"abstract":"The article presents new data on the significance of vitamin D deficiency in the formation of endometrial structure disorders in women with luteal phase defect and unrupted follicle syndrome. Disorders of the menstrual cycle caused by a progesterone deficiency from the position of influence on the onset and progression of pregnancy are considered. Fertility of women, especially with endocrine imbalances, deserves special attention, since the frequency of early pregnancy loss in them is about 67 %. Aim: study of the effects of vitamin D deficiency and progesterone deficiency in the luteinized unruptured follicle syndrome (Geis-Genes) and the luteal phase defect on the reproductive potential of women. Materials and methods: a hormonal study, determination of vitamin D levels, a sonographic study conducted on days 5–7 and 21–24 of the menstrual cycle in the periconceptional supplementation program of 45 women with luteal phase deficiency (LPD) and luteinized unruptured follicle syndrome (LUF). Results: The relationship between the content of vitamin D and the hormonal levels of the reproductive system of women with LPD and LUF is traced. The role of reducing vitamin D levels in the formation of hormonal imbalances in women of reproductive age has been established. Conclusions: – progesterone deficiency entails changes in various regulatory substances of the reproductive system, and therefore the periconceptional supplementation must be individual. – The introduction of the definition of vitamin D in the periconceptional supplementation plan and its correction are justified and necessary to improve the structure of the endometrium and its secretory transformation in women with progesterone imbalance.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84035895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00744
S. Hryshchuk
In case of combined pharmacotherapy, between drugs can occur interactions, which can be dangerous to the life and health of patients. Such combinations of drugs are considered to be one of the serious clinical problems, and are considered as errors that can be prevented. Therefore, it is relevant to determine the frequency of such appointments for further work on their prevention. Aim of the research. Determine the structure and prevalence of the appointments of potentially dangerous combinations of drugs in the treatment of patients under inpatient treatment for further optimization of pharmacotherapy. Materials and methods. In the automated mode, the lists of medical appointments of patients who were under inpatient treatment of 30 health facilities of Zhytomyr region in 2017 were monitored for the purpose of the simultaneous use of drugs, the interaction of which is dangerous. For further analysis by the levels of clinical significance using the Drug Interaction Checker system, the "Major" (hazardous) and "Moderate" (significant) combinations were selected on the Internet resource www.drugs.com. The frequency of the appointments of dangerous combinations, their prevalence by the nosological classes and the profiles of the offices of medical institutions was determined. Results. As a result of the monitoring of 69405 cases, it was found that the average number of prescriptions per patient was 5.6±1.2 titles. In 1390 cases of treatment (2.0 % of 69405) simultaneously prescribed medicines, the interaction of which could be dangerous. In the structure of dangerous combinations, 81.5 % is taken with the simultaneous administration of drugs containing active substances ketorolac and pentoxifylline. The frequency of potentially dangerous appointments was greatest in the treatment of patients with diseases of the blood and blood-forming organs (D50-D89) – 11.6 %, in diseases of the musculoskeletal system and connective tissue (M00-M99) – 7.7 %, in diseases of the nervous system – 5.0 % of all cases of treatment.
{"title":"RESULTS OF MONITORING OF PRESCRIPTION OF POTENTIALLY DANGEROUS COMBINATIONS OF DRUGS IN PHARMACOTHERAPY UNDER INPATIENT TREATMENT","authors":"S. Hryshchuk","doi":"10.21303/2585-663.2018.00744","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00744","url":null,"abstract":"In case of combined pharmacotherapy, between drugs can occur interactions, which can be dangerous to the life and health of patients. Such combinations of drugs are considered to be one of the serious clinical problems, and are considered as errors that can be prevented. Therefore, it is relevant to determine the frequency of such appointments for further work on their prevention. Aim of the research. Determine the structure and prevalence of the appointments of potentially dangerous combinations of drugs in the treatment of patients under inpatient treatment for further optimization of pharmacotherapy. Materials and methods. In the automated mode, the lists of medical appointments of patients who were under inpatient treatment of 30 health facilities of Zhytomyr region in 2017 were monitored for the purpose of the simultaneous use of drugs, the interaction of which is dangerous. For further analysis by the levels of clinical significance using the Drug Interaction Checker system, the \"Major\" (hazardous) and \"Moderate\" (significant) combinations were selected on the Internet resource www.drugs.com. The frequency of the appointments of dangerous combinations, their prevalence by the nosological classes and the profiles of the offices of medical institutions was determined. Results. As a result of the monitoring of 69405 cases, it was found that the average number of prescriptions per patient was 5.6±1.2 titles. In 1390 cases of treatment (2.0 % of 69405) simultaneously prescribed medicines, the interaction of which could be dangerous. In the structure of dangerous combinations, 81.5 % is taken with the simultaneous administration of drugs containing active substances ketorolac and pentoxifylline. The frequency of potentially dangerous appointments was greatest in the treatment of patients with diseases of the blood and blood-forming organs (D50-D89) – 11.6 %, in diseases of the musculoskeletal system and connective tissue (M00-M99) – 7.7 %, in diseases of the nervous system – 5.0 % of all cases of treatment.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83031418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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