Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00739
T. Yarnykh, O. Rukhmakova
The aim of this work was the development of technology of rectal suppositories of immunomodulatory action for children and their standardization. To obtain suppositories, a licorice root extract, chamomile and tea tree essential oils were used. Given that the introduction of ingredients in a dissolved form is preferable, we studied the possibility of using emulsion suppository bases. To evaluate the compositions, the homogeneity and colloidal stability of the suppositories obtained were studied. Estimation of homogeneity, melting temperature, decay time, average mass was carried out according to the State Pharmacopoeia. To confirm the authenticity of the active ingredients, a chromatography method was used. The quantitative determination of glycyrrhizin acid was carried out by spectrophotometric method. Suppositories were prepared by the pouring method. The licorice root extract was added to the suppository when dissolved in the hydrophilic phase of the base. Essential oils of chamomile and tea tree were introduced when a solid fat of type A dissolved in a melt, at a temperature of (42.0±2.0) ºC. Optimum indices for the criteria of “homogeneity” and “colloidal stability” were noted for the samples of the composition: purified water, polysorbate-80, lecithin, solid fat type A. To prove the authenticity of the licorice root extract in suppositories, the chloroform-methanol-water system was optimal (26:14:3). Identification of essential oils on a gas chromatograph showed that on the chromatogram the peaks and retention times of the solution under study coincide with the peaks and retention times of the reference solutions. The conducted studies of quantitative determination of glycyrrhizin acid have shown that its content in one suppository is not less than 0.035 g in terms of glycyram.
{"title":"THE ASPECTS OF DEVELOPMENT AND STANDARDIZATION OF CHILDREN’S SUPPOSITORIES WITH EXTRACT OF LICORICE ROOT","authors":"T. Yarnykh, O. Rukhmakova","doi":"10.21303/2585-663.2018.00739","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00739","url":null,"abstract":"The aim of this work was the development of technology of rectal suppositories of immunomodulatory action for children and their standardization. To obtain suppositories, a licorice root extract, chamomile and tea tree essential oils were used. Given that the introduction of ingredients in a dissolved form is preferable, we studied the possibility of using emulsion suppository bases. To evaluate the compositions, the homogeneity and colloidal stability of the suppositories obtained were studied. Estimation of homogeneity, melting temperature, decay time, average mass was carried out according to the State Pharmacopoeia. To confirm the authenticity of the active ingredients, a chromatography method was used. The quantitative determination of glycyrrhizin acid was carried out by spectrophotometric method. Suppositories were prepared by the pouring method. The licorice root extract was added to the suppository when dissolved in the hydrophilic phase of the base. Essential oils of chamomile and tea tree were introduced when a solid fat of type A dissolved in a melt, at a temperature of (42.0±2.0) ºC. Optimum indices for the criteria of “homogeneity” and “colloidal stability” were noted for the samples of the composition: purified water, polysorbate-80, lecithin, solid fat type A. To prove the authenticity of the licorice root extract in suppositories, the chloroform-methanol-water system was optimal (26:14:3). Identification of essential oils on a gas chromatograph showed that on the chromatogram the peaks and retention times of the solution under study coincide with the peaks and retention times of the reference solutions. The conducted studies of quantitative determination of glycyrrhizin acid have shown that its content in one suppository is not less than 0.035 g in terms of glycyram.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82316307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00774
O. Buznytska
Non-alcoholic fatty liver disease occurs in most obese people, the main pathway of which is the process of fibrogenesis. This disorder is currently classified into two types: hepatic steatosis and nonalcoholic steatohepatitis. Hepatic steatosis is a reversible condition in which large vacuoles of triglyceride fat accumulate in the liver cells, causing nonspecific inflammation. Most people with this condition experience few, if any, symptoms, and it does not usually lead to scarring or serious liver damage. The majority of patients with nonalcoholic fatty liver disease have this type. Nonalcoholic steatohepatitis is the more severe, progressive form that involves not only fat accumulation (steatosis) in the liver but also inflammation. Steatohepatitis can lead to fibrosis and eventually to cirrhosis, which is severe scarring that can lead to liver failure. The real frequency of the prevalence of the disease is difficult to establish, due to the insufficient use of non-invasive screening diagnostic methods, through which it is possible to detect the initial forms of the disease. The aim: to study the diagnostic significance of the serum biomarkers of liver fibrogenesis in adolescents with obesity. Methods. On the base of the Department of Endocrinology, SI “Institute of children and adolescence health care of NAMS” (Kharkov) 226 patients with obesity aged 8–18 years were examined. Investigation of liver fibrosis consisted of measurement in blood the levels of fibronectin, collagen type IV, N-terminal propeptides and C-terminal telopeptides of type I collagen by IFA method. Results. The study of liver fibrogenesis revealed a significant increase in levels of type IV collagen and fibronectin in children with obesity (p<0.05). As diagnostic criteria for two physiologically diverse processes – fibrogenesis and fibrolysis, the levels of N-terminal propeptides and C-terminal telopeptides of type I collagen, respectively, were determined. The serum level of N-terminal propeptides of type I collagen significantly exceeds the normal values in all children with obesity, in contrast to the children of the control group (p<0.05). Conclusion. It has been established that a biochemical method for determining the level of type IV collagen, fibronectin, N-terminal propeptides and C-terminal telopeptides of type I collagen has a high sensitivity for the diagnosis of liver fibrogenesis.
{"title":"ADVANTAGES OF BIOCHEMICAL METHODS OF DIAGNOSING FIBROSIS IN NON-ALCOHOLIC FATTY LIVER DISEASE IN ADOLESCENTS WITH OBESITY","authors":"O. Buznytska","doi":"10.21303/2585-663.2018.00774","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00774","url":null,"abstract":"Non-alcoholic fatty liver disease occurs in most obese people, the main pathway of which is the process of fibrogenesis. This disorder is currently classified into two types: hepatic steatosis and nonalcoholic steatohepatitis. Hepatic steatosis is a reversible condition in which large vacuoles of triglyceride fat accumulate in the liver cells, causing nonspecific inflammation. Most people with this condition experience few, if any, symptoms, and it does not usually lead to scarring or serious liver damage. The majority of patients with nonalcoholic fatty liver disease have this type. Nonalcoholic steatohepatitis is the more severe, progressive form that involves not only fat accumulation (steatosis) in the liver but also inflammation. Steatohepatitis can lead to fibrosis and eventually to cirrhosis, which is severe scarring that can lead to liver failure. The real frequency of the prevalence of the disease is difficult to establish, due to the insufficient use of non-invasive screening diagnostic methods, through which it is possible to detect the initial forms of the disease. The aim: to study the diagnostic significance of the serum biomarkers of liver fibrogenesis in adolescents with obesity. Methods. On the base of the Department of Endocrinology, SI “Institute of children and adolescence health care of NAMS” (Kharkov) 226 patients with obesity aged 8–18 years were examined. Investigation of liver fibrosis consisted of measurement in blood the levels of fibronectin, collagen type IV, N-terminal propeptides and C-terminal telopeptides of type I collagen by IFA method. Results. The study of liver fibrogenesis revealed a significant increase in levels of type IV collagen and fibronectin in children with obesity (p<0.05). As diagnostic criteria for two physiologically diverse processes – fibrogenesis and fibrolysis, the levels of N-terminal propeptides and C-terminal telopeptides of type I collagen, respectively, were determined. The serum level of N-terminal propeptides of type I collagen significantly exceeds the normal values in all children with obesity, in contrast to the children of the control group (p<0.05). Conclusion. It has been established that a biochemical method for determining the level of type IV collagen, fibronectin, N-terminal propeptides and C-terminal telopeptides of type I collagen has a high sensitivity for the diagnosis of liver fibrogenesis.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83508664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00779
I. Zhabchenko, S. Tertychnaya-Telyuk
Aim of the research is to study identification of peculiar issues associated with psycho-emotional state and the related stress-associated hormones and micronutrients in pregnant-displace individuals for further improvement of programmes for antenatal research. Materials and methods. In order to reach the research objective, a dynamic prospective clinical-paraclinical examination of 96 pregnant women (experimental group) – temporary displaced individuals with gestation period of more than 22 weeks who have been treated and gave birth to their children in the Central Municipal Hospital in Rubizhne and Perinatal Centre in Severodonetsk, Lugansk region. The control group consists of 39 randomly selected pregnant women for the purposes of prospective research that are living in the areas under the control of Ukraine government. All pregnant women were examined (clinical-laboratory and special midwifery examinations), the evaluation of psycho-emotional state of examined pregnant women was conducted with the help of screening by performing clinical interviews together with routine questioning. The questionnaires that contained the questions of Spielberg’s tests in the modification of Khanin were composed in advance. Concentration of stress-associated hormones in the blood serum, Ca and Mg was identified using the method of solid-phase immuno-ferment analysis. Results. According to the Spielberg’s test, the average score of reactive anxiety in the experimental group exceeded the analogous indicator in the control group by 1.6 times. The indicators of personal anxiety exceeded the normal indicator in the control group by 2.2 times. Of the pregnant women in the experimental group had the concentration of cortisol in the blood serum exceeded the upper physiological standard by 5.5 % and exceeded the analogous indicator in the control group by 43.4 %. The increase concentration of prolactin was yet observed in the II trimester of pregnancy if compared to the upper level of physiological standard by 13.3 %; if compared to the average indicator of this hormone in the control group - by 20.3 %. In the III trimester - concentration in both groups was within normal ranges, but in the experimental group this indicator was higher by 10.5 %. In the experimental group of pregnant women the concentration of Ca in the blood serum in comparison to the physiological standard was higher by 19.2 % and by 9.6 % higher if compared to the analogous indicator in the control group of pregnant women. At the same time, the pregnant women in the experimental group have low concentration of Mg. Conclusions. Taking into account the identified patterns, it is useful to categorise the pregnant women from among the internally displaced individuals as the group of high risk with respect to the development of obstetric and perinatal complications.
{"title":"POSTTRAUMATIC SYNDROME DURING PREGNANCY: EXPECTATIONS AND REALITY","authors":"I. Zhabchenko, S. Tertychnaya-Telyuk","doi":"10.21303/2585-663.2018.00779","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00779","url":null,"abstract":"Aim of the research is to study identification of peculiar issues associated with psycho-emotional state and the related stress-associated hormones and micronutrients in pregnant-displace individuals for further improvement of programmes for antenatal research. Materials and methods. In order to reach the research objective, a dynamic prospective clinical-paraclinical examination of 96 pregnant women (experimental group) – temporary displaced individuals with gestation period of more than 22 weeks who have been treated and gave birth to their children in the Central Municipal Hospital in Rubizhne and Perinatal Centre in Severodonetsk, Lugansk region. The control group consists of 39 randomly selected pregnant women for the purposes of prospective research that are living in the areas under the control of Ukraine government. All pregnant women were examined (clinical-laboratory and special midwifery examinations), the evaluation of psycho-emotional state of examined pregnant women was conducted with the help of screening by performing clinical interviews together with routine questioning. The questionnaires that contained the questions of Spielberg’s tests in the modification of Khanin were composed in advance. Concentration of stress-associated hormones in the blood serum, Ca and Mg was identified using the method of solid-phase immuno-ferment analysis. Results. According to the Spielberg’s test, the average score of reactive anxiety in the experimental group exceeded the analogous indicator in the control group by 1.6 times. The indicators of personal anxiety exceeded the normal indicator in the control group by 2.2 times. Of the pregnant women in the experimental group had the concentration of cortisol in the blood serum exceeded the upper physiological standard by 5.5 % and exceeded the analogous indicator in the control group by 43.4 %. The increase concentration of prolactin was yet observed in the II trimester of pregnancy if compared to the upper level of physiological standard by 13.3 %; if compared to the average indicator of this hormone in the control group - by 20.3 %. In the III trimester - concentration in both groups was within normal ranges, but in the experimental group this indicator was higher by 10.5 %. In the experimental group of pregnant women the concentration of Ca in the blood serum in comparison to the physiological standard was higher by 19.2 % and by 9.6 % higher if compared to the analogous indicator in the control group of pregnant women. At the same time, the pregnant women in the experimental group have low concentration of Mg. Conclusions. Taking into account the identified patterns, it is useful to categorise the pregnant women from among the internally displaced individuals as the group of high risk with respect to the development of obstetric and perinatal complications.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89360380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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