Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00742
P. Zadorozhnii, Ihor O. Pokotylo
Alzheimer's disease is a neurodegenerative disease characterized by pathological features of neurofibrillary tangles and β-amyloid plaques in the cerebral cortex. In Alzheimer's disease, tau protein undergoes excess phosphorylation, due to which its threads begin to merge and form neurofibrillary tangles within nerve cells. It has been shown that glycogen synthase kinase-3β is a key factor in the phosphorylation of tau protein, its increased activity leading to pathologies of neurofibrillary tangles and, consequently, to neurodegenerative changes in the brain. In this connection, the search for effective inhibitors of GSK-3β is a very important and urgent task, for their further use in the treatment of Alzheimer's disease. Aim of research.The aim of this study is to search new inhibitors of GSK-3β among N-amidoalkylated derivatives of 2-amino-1,3,4-oxadiazole by molecular docking methods. Materials and methods. We have carried out geometry optimization of analyzed structures within PM3 semi-empirical method, and GSK-3β molecular docking using software ArgusLab 4.0.1. The three-dimensional crystal structure of co-crystallizer GSK-3β and inhibitor has been loaded from the data bank of protein molecules (PDB ID: 3F7Z). Results. In this study it has been shown that the structures being studied mainly form stronger complexes with the enzyme compared to the known inhibitor. Based on the results of molecular docking, the compounds leaders N-(((5-(2-bromophenyl)-1,3,4-oxadiazol-2-yl)amino)methyl)benzamide and 2,4-dichloro-N-(2,2,2-trichloro-1-((5-(p-tolyl)-1,3,4-oxadiazol-2-yl)amino)ethyl)benzamide have been chosen. The structures of the compounds leaders have been tested for compliance with Lipinski criteria. Conclusions. Proposed compounds leaders can be recommended for further studies in the treatment of Alzheimer's disease. Despite the good results obtained in silico analysis, it is mandatory to perform biological tests in vitro and in vivo.
{"title":"MOLECULAR DOCKING STUDIES OF SOME N-AMIDOALKYLATED DERIVATIVES OF 2-AMINO-1,3,4-OXADIAZOLE AS POTENTIAL INHIBITORS OF GLYCOGEN SYNTHASE KINASE-3β","authors":"P. Zadorozhnii, Ihor O. Pokotylo","doi":"10.21303/2585-663.2018.00742","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00742","url":null,"abstract":"Alzheimer's disease is a neurodegenerative disease characterized by pathological features of neurofibrillary tangles and β-amyloid plaques in the cerebral cortex. In Alzheimer's disease, tau protein undergoes excess phosphorylation, due to which its threads begin to merge and form neurofibrillary tangles within nerve cells. It has been shown that glycogen synthase kinase-3β is a key factor in the phosphorylation of tau protein, its increased activity leading to pathologies of neurofibrillary tangles and, consequently, to neurodegenerative changes in the brain. In this connection, the search for effective inhibitors of GSK-3β is a very important and urgent task, for their further use in the treatment of Alzheimer's disease. Aim of research.The aim of this study is to search new inhibitors of GSK-3β among N-amidoalkylated derivatives of 2-amino-1,3,4-oxadiazole by molecular docking methods. Materials and methods. We have carried out geometry optimization of analyzed structures within PM3 semi-empirical method, and GSK-3β molecular docking using software ArgusLab 4.0.1. The three-dimensional crystal structure of co-crystallizer GSK-3β and inhibitor has been loaded from the data bank of protein molecules (PDB ID: 3F7Z). Results. In this study it has been shown that the structures being studied mainly form stronger complexes with the enzyme compared to the known inhibitor. Based on the results of molecular docking, the compounds leaders N-(((5-(2-bromophenyl)-1,3,4-oxadiazol-2-yl)amino)methyl)benzamide and 2,4-dichloro-N-(2,2,2-trichloro-1-((5-(p-tolyl)-1,3,4-oxadiazol-2-yl)amino)ethyl)benzamide have been chosen. The structures of the compounds leaders have been tested for compliance with Lipinski criteria. Conclusions. Proposed compounds leaders can be recommended for further studies in the treatment of Alzheimer's disease. Despite the good results obtained in silico analysis, it is mandatory to perform biological tests in vitro and in vivo.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89832497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00777
I. Shuvalova, Lee Sang-kyu
The aim of the article was to study of the influence of the apparatus “MOXI” as an alternative method for the correction of arterial hypertension and improving the quality of life of patients using acupuncture reflexotherapy. Materials and methods. The study was conducted in 36 patients with arterial hypertension of 1 stage, low risk, with the experience of the disease from 1 to 2.5 years, without taking antihypertensive therapy. The average age of patients was 41.3±2.6 years. The state of the psycho-emotional sphere was assessed by the SAN test. The determination of the general non-specific adaptation reaction according to Garkavi-Kvakina-Ukolova was carried out. Control of blood pressure and pulse, was carried out in the center, and by the diaries of self-control. We calculated the arithmetic mean of blood pressure for the day and week. Baseline blood pressure was 152/106 mmHg when measured at the center and 149/102 mmHg at home. Results. The obtained results indicate that the adaptogenic set of acupuncture points allows you to simultaneous selective regulation of the function of organs and systems according to the principle of segmental and vegetative innervation. After a course of preventive recovery, a statistically significant (p<0.05) normalization of well-being indicators according to the SAN test was noted from 4.0 to 4.6 points, activity from 3.9 to 4.4 points, mood from 3.9 to 4.7 points. All patients showed a pronounced reduction of complaints and autonomic disorders. In addition, we revealed improvement in the functional state of the cardiovascular system. So, systolic pressure decreased from 152±1.5 mmHg up to 134±2.4 mmHg, and diastolic from 106±0.7 mmHg to 82±1.8 mmHg. Conclusions. As a result of the prophylactic treatment, all patients registered a normal type of adaptive response, since the training reaction was detected in 44.4 % of cases, the calm activation reaction was 22.2 %, in the area of increased activation - 33.4 %. The use of the “MOXI” device in the treatment of patients with arterial hypertension of 1 type, with a low level of risk gives a positive therapeutic effect in the normalization of the psycho-emotional state of patients. It normalizes non-specific adaptation reactions of the body, and allows you to achieve a decrease in blood pressure to normal levels.
{"title":"ALTERNATIVE METHOD FOR TREATING ARTERIAL HYPERTENSION WITH THE USE OF THE «MOXI» DEVICE","authors":"I. Shuvalova, Lee Sang-kyu","doi":"10.21303/2585-663.2018.00777","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00777","url":null,"abstract":"The aim of the article was to study of the influence of the apparatus “MOXI” as an alternative method for the correction of arterial hypertension and improving the quality of life of patients using acupuncture reflexotherapy. Materials and methods. The study was conducted in 36 patients with arterial hypertension of 1 stage, low risk, with the experience of the disease from 1 to 2.5 years, without taking antihypertensive therapy. The average age of patients was 41.3±2.6 years. The state of the psycho-emotional sphere was assessed by the SAN test. The determination of the general non-specific adaptation reaction according to Garkavi-Kvakina-Ukolova was carried out. Control of blood pressure and pulse, was carried out in the center, and by the diaries of self-control. We calculated the arithmetic mean of blood pressure for the day and week. Baseline blood pressure was 152/106 mmHg when measured at the center and 149/102 mmHg at home. Results. The obtained results indicate that the adaptogenic set of acupuncture points allows you to simultaneous selective regulation of the function of organs and systems according to the principle of segmental and vegetative innervation. After a course of preventive recovery, a statistically significant (p<0.05) normalization of well-being indicators according to the SAN test was noted from 4.0 to 4.6 points, activity from 3.9 to 4.4 points, mood from 3.9 to 4.7 points. All patients showed a pronounced reduction of complaints and autonomic disorders. In addition, we revealed improvement in the functional state of the cardiovascular system. So, systolic pressure decreased from 152±1.5 mmHg up to 134±2.4 mmHg, and diastolic from 106±0.7 mmHg to 82±1.8 mmHg. Conclusions. As a result of the prophylactic treatment, all patients registered a normal type of adaptive response, since the training reaction was detected in 44.4 % of cases, the calm activation reaction was 22.2 %, in the area of increased activation - 33.4 %. The use of the “MOXI” device in the treatment of patients with arterial hypertension of 1 type, with a low level of risk gives a positive therapeutic effect in the normalization of the psycho-emotional state of patients. It normalizes non-specific adaptation reactions of the body, and allows you to achieve a decrease in blood pressure to normal levels.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80025316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00782
N. Bondarenko, A. Aksonova
Aim of the work. The focus of this study was to analyze the frequency of fetal intrauterine symptomatic realization as a result of B19 parvovirus infection of pregnant women during I and II gestation periods, with the combination of determination the levels of alpha-fetoprotein in maternal serum blood and histopathological investigation of placenta in cases of negative effects for infected fetuses. Materials and methods. A total of 478 pregnant women were at risk of infection and screened for parvovirus B19. The study was based on serological, morphological and virological testing of fetuses from mothers with confirmed parvovirus B19 infection. Infection of pregnant women was detected by serological diagnosis of specific antibodies IgM and IgG to parvovirus B19. Intrauterine fetal infection was confirmed by polymerase chain reaction method. Levels of alpha-fetoprotein in meternal venous blood were detected using an immunochemical test method. An antenatal diagnosis of hydrops fetalis was confirmed by ultrasound scanning. Placenta from the 8 infected fetuses was studied histologically with hematoxylin and eosin staining using electron microscope during gestational periods of 12-22 weeks. Statistical analysis of the obtained data was carried out using nonparametric statistics with the definition of Fisher's criterion. Results and discussion. The average gestational period of symptomatic manifestations of intrauterine fetal infection was 19.9±0.5 weeks of gestation. It was established that in 33 of cases were confirmed the intrauterine infection due to parvovirus B19 by using the morphological placenta tissue analysis. The research found that the levels of maternal serum alpha-fetoprotein was significantly higher (in 2.17 times) in group of fetuses who suffered from fetal hydrops and in cases of intrauterine fetal death before detecting the general echographic sings of fetal failure (p˂0.05). It will be very important to compare this indicator with the Doppler ultrasound measurements of the human fetal middle cerebral artery peak systolic velocity (MCA PSV) and blood flow parameters in the fetal ductus venosus and to predict the possibility of poor perinatal outcomes. It was identified a cytopathic effect in placental cells viral origin and detected in cells of the cytotrophoblast, syncytiotrophoblast, endothelial cells, and blood cells. Conclusions. Parvovirus B19 fractions were visualized in 100 % of cases of miscarriages with fetal hydrops and placental edema during 12-22 weeks of gestation, while acute maternal parvovirus infection (with the detection of IgM against to parvovirus B19) was diagnosed by immunoassay only in 62.5 % of cases. Detection of B19 particles in placental tissue from fetuses with non-immune origin hydrops is an auxiliary method in the diagnosis of vertical transmission of parvovirus during pregnancy. It is more preferable to estimate the levels of alpha-fetoprotein in maternal serum blood in case of parvovirus B19 affected
{"title":"PLACENTAL CYTOPATHIC DAMAGE FACTOR DUE TO PARVOVIRUS B19 – AS A HALLMARK OF INTRAUTERINE SYMPTOMATHY OF INFECTED FETUS","authors":"N. Bondarenko, A. Aksonova","doi":"10.21303/2585-663.2018.00782","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00782","url":null,"abstract":"Aim of the work. The focus of this study was to analyze the frequency of fetal intrauterine symptomatic realization as a result of B19 parvovirus infection of pregnant women during I and II gestation periods, with the combination of determination the levels of alpha-fetoprotein in maternal serum blood and histopathological investigation of placenta in cases of negative effects for infected fetuses. Materials and methods. A total of 478 pregnant women were at risk of infection and screened for parvovirus B19. The study was based on serological, morphological and virological testing of fetuses from mothers with confirmed parvovirus B19 infection. Infection of pregnant women was detected by serological diagnosis of specific antibodies IgM and IgG to parvovirus B19. Intrauterine fetal infection was confirmed by polymerase chain reaction method. Levels of alpha-fetoprotein in meternal venous blood were detected using an immunochemical test method. An antenatal diagnosis of hydrops fetalis was confirmed by ultrasound scanning. Placenta from the 8 infected fetuses was studied histologically with hematoxylin and eosin staining using electron microscope during gestational periods of 12-22 weeks. Statistical analysis of the obtained data was carried out using nonparametric statistics with the definition of Fisher's criterion. Results and discussion. The average gestational period of symptomatic manifestations of intrauterine fetal infection was 19.9±0.5 weeks of gestation. It was established that in 33 of cases were confirmed the intrauterine infection due to parvovirus B19 by using the morphological placenta tissue analysis. The research found that the levels of maternal serum alpha-fetoprotein was significantly higher (in 2.17 times) in group of fetuses who suffered from fetal hydrops and in cases of intrauterine fetal death before detecting the general echographic sings of fetal failure (p˂0.05). It will be very important to compare this indicator with the Doppler ultrasound measurements of the human fetal middle cerebral artery peak systolic velocity (MCA PSV) and blood flow parameters in the fetal ductus venosus and to predict the possibility of poor perinatal outcomes. It was identified a cytopathic effect in placental cells viral origin and detected in cells of the cytotrophoblast, syncytiotrophoblast, endothelial cells, and blood cells. Conclusions. Parvovirus B19 fractions were visualized in 100 % of cases of miscarriages with fetal hydrops and placental edema during 12-22 weeks of gestation, while acute maternal parvovirus infection (with the detection of IgM against to parvovirus B19) was diagnosed by immunoassay only in 62.5 % of cases. Detection of B19 particles in placental tissue from fetuses with non-immune origin hydrops is an auxiliary method in the diagnosis of vertical transmission of parvovirus during pregnancy. It is more preferable to estimate the levels of alpha-fetoprotein in maternal serum blood in case of parvovirus B19 affected ","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83329739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00750
E. Stroy
It has been proven that the functions of vitamin D are not limited to the control of calcium-phosphorus metabolism, but its other pleiotropic effects are being studied. The aim of our study was to study vitamin D status and its effect on the lipid profile in children of risk group for the development of dyslipoproteinemia. Materials and methods 30 children aged 6–7 years (mean age 6.4±0.6 years) who were born to women with diabetes were examined. The control group – 30 children 6–7 years old (6.5±0.5 years) from healthy mothers. An assessment of physical development was carried out with the calculation of body mass index. We studied lipid metabolism indicators - total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol (mmol / l) in blood, atherogenic index. All children were determined with the content of 25-OH-D in the serum, the level of glucose. Results 10 (33.3 %) children from mothers with diabetes are overweight, 5 (16.7 %) – obesity, which is 2 times higher than in the control group. The fasting glucose level in all the examined children from mothers with diabetes was within physiological fluctuations. The level of 25-OH-D in children from mothers with diabetes mellitus, compared with the control group, was significantly reduced. Dyslipoproteinemia was found in children from diabetic mothers: a significant increase in serum triglyceride and cholesterol levels, higher levels of low-density lipoprotein cholesterol and lower levels of high-density lipoprotein cholesterol than in the control group (p <0.05), atherogenic index was significantly higher. Prospects for further research are to explore the possibility of using vitamin D for the primary prevention and treatment of metabolic syndrome, diabetes in children. Conclusions Vitamin D deficiency in the body of the surveyed can be one of the causes of their dyslipidemia, in addition, overweight and obesity are also risk factors for the development of atherosclerosis, metabolic syndrome. Children at risk need dynamic monitoring of anthropometric indicators, control of body mass index, lipid and carbohydrate blood profile, level 25-OH-D.
{"title":"EFFECT OF VITAMIN D STATUS ON LIPID METABOLISM OF CHILDREN","authors":"E. Stroy","doi":"10.21303/2585-663.2018.00750","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00750","url":null,"abstract":"It has been proven that the functions of vitamin D are not limited to the control of calcium-phosphorus metabolism, but its other pleiotropic effects are being studied. The aim of our study was to study vitamin D status and its effect on the lipid profile in children of risk group for the development of dyslipoproteinemia. Materials and methods 30 children aged 6–7 years (mean age 6.4±0.6 years) who were born to women with diabetes were examined. The control group – 30 children 6–7 years old (6.5±0.5 years) from healthy mothers. An assessment of physical development was carried out with the calculation of body mass index. We studied lipid metabolism indicators - total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol (mmol / l) in blood, atherogenic index. All children were determined with the content of 25-OH-D in the serum, the level of glucose. Results 10 (33.3 %) children from mothers with diabetes are overweight, 5 (16.7 %) – obesity, which is 2 times higher than in the control group. The fasting glucose level in all the examined children from mothers with diabetes was within physiological fluctuations. The level of 25-OH-D in children from mothers with diabetes mellitus, compared with the control group, was significantly reduced. Dyslipoproteinemia was found in children from diabetic mothers: a significant increase in serum triglyceride and cholesterol levels, higher levels of low-density lipoprotein cholesterol and lower levels of high-density lipoprotein cholesterol than in the control group (p <0.05), atherogenic index was significantly higher. Prospects for further research are to explore the possibility of using vitamin D for the primary prevention and treatment of metabolic syndrome, diabetes in children. Conclusions Vitamin D deficiency in the body of the surveyed can be one of the causes of their dyslipidemia, in addition, overweight and obesity are also risk factors for the development of atherosclerosis, metabolic syndrome. Children at risk need dynamic monitoring of anthropometric indicators, control of body mass index, lipid and carbohydrate blood profile, level 25-OH-D.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80301172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00783
R. Sahaidak-Nikitiuk, S. Velma
Aim: to determine the factors of influence on the magnitude of logistics costs and developing measures to minimize them. Materials and methods. The methods used in the study include the expert, systematic method of scientific knowledge, structural analysis, the method of abstract modeling. An expert method was conducted with the help of questionnaires of specialists of pharmaceutical companies, pharmacies and wholesale pharmaceutical companies. 118 respondents participated in the survey. The degree of consistency of expert opinions was determined with the help of the coefficient of concordance, which is 0.8374 (the normative value of the coefficient of concordance is 0.75), the Pearson criterion also exceeds the table value for the corresponding number of degrees of freedom, which testifies to the non-randomness of their judgments. Results. The factors influencing the amount of logistics costs are determined. The internal factors of influence include the activities of the subject of the pharmaceutical industry, assortment structure and structure of the use of substances and materials, the magnitude of stocks, etc. External factors of influence are the level of prices for logistics services provided by outside organizations; rate of transport tax; environmental taxes etc.; competitors suppliers of substances and materials; consumers; intermediaries; legislative base, etc. The conducted questioning of specialists and taking into account the current experience of leading foreign pharmaceutical companies allowed to determine the main measures to minimize logistics costs for all parts of the logistics system - procurement, production, transportation, storage, distribution and recycling, and types of flows - material, financial, information, labor. Conclusions. Based on the carried out research, the factors influencing the magnitude of logistics costs, which experts are divided into external and internal, are determined. The matrix of the components of LV savings is proposed, which takes into account the type of flow and the links of the logistic system. The constructed matrix allows to minimize the amount of LV, which helps to reduce the price of drugs for the end user.
{"title":"SUBSTANTIATION OF THE MECHANISM OF LOGISTIC EXPENDITURE MINIMIZATION BY SUBJECTS OF THE PHARMACEUTICAL INDUSTRY","authors":"R. Sahaidak-Nikitiuk, S. Velma","doi":"10.21303/2585-663.2018.00783","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00783","url":null,"abstract":"Aim: to determine the factors of influence on the magnitude of logistics costs and developing measures to minimize them. Materials and methods. The methods used in the study include the expert, systematic method of scientific knowledge, structural analysis, the method of abstract modeling. An expert method was conducted with the help of questionnaires of specialists of pharmaceutical companies, pharmacies and wholesale pharmaceutical companies. 118 respondents participated in the survey. The degree of consistency of expert opinions was determined with the help of the coefficient of concordance, which is 0.8374 (the normative value of the coefficient of concordance is 0.75), the Pearson criterion also exceeds the table value for the corresponding number of degrees of freedom, which testifies to the non-randomness of their judgments. Results. The factors influencing the amount of logistics costs are determined. The internal factors of influence include the activities of the subject of the pharmaceutical industry, assortment structure and structure of the use of substances and materials, the magnitude of stocks, etc. External factors of influence are the level of prices for logistics services provided by outside organizations; rate of transport tax; environmental taxes etc.; competitors suppliers of substances and materials; consumers; intermediaries; legislative base, etc. The conducted questioning of specialists and taking into account the current experience of leading foreign pharmaceutical companies allowed to determine the main measures to minimize logistics costs for all parts of the logistics system - procurement, production, transportation, storage, distribution and recycling, and types of flows - material, financial, information, labor. Conclusions. Based on the carried out research, the factors influencing the magnitude of logistics costs, which experts are divided into external and internal, are determined. The matrix of the components of LV savings is proposed, which takes into account the type of flow and the links of the logistic system. The constructed matrix allows to minimize the amount of LV, which helps to reduce the price of drugs for the end user.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80290349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00762
Y. Vyzhga
The activity of the juvenile idiopathic arthritis evaluates the speed of the process progression, intense of its clinical manifestation, effect of the carried treatment. The aim of our study was to estimate changes of the inflammatory response signs as well as content of NF-kB due to basic therapy of the JIA. Materials and methods. At our study, we had checked 68 children with JIA, who passed their treatment at Vinnytsya regional children’s hospital within the period from 2011 to 2014 years. Results. At the patients we studied currency of the JIA was characterized with articular variant of the disease, mainly in monoarthritis type. Laboratory activity of the inflammatory response characterized with increased content of the C-reactive protein (71.2±3.7 %), inflammatory cytokines – ІL-1β (54.8±4.1 %) and ІL-6 (56.2±2.4 %), as well high quantity of the nuclear factor-kB (70.5±3.1 %). Currency of the JIA in children characterized with high increasing of the inflammatory response signs especially C-reactive protein (6.55(4.2;9.8)), ІL-1β (7.3(3.5;11.9)), ІL-6 (6.8(4.5;10.6)) and NF-kB (6.76 (4.8; 9.1)), that are in correlative connections with clinical signs (number of the injured and swelled joints, evaluation of the general condition of the child according to doctors and own response) of the disease activity (rxy = +0.34 up to 0.62, p< 0.01). Conclusion. During the managing of the basic therapy in children with JIA we estimated decreasing of the IL-1β content in patients at the background of methotrexate administration (38.7±3.7 %), at the second group with use of sulfasalazine (28.5±3.5 %) and the third with leflunomide prescription (29.1±5.1 %), but significant decreasing of the ІL-6 content, that is one of the main inflammatory mediators and as well NF-kB was found just in group of the patients with methotrexate administration (on 36.3±3.8 % and 32.4±2.4 % for NF-kB).
{"title":"EVALUATION OF THE MAIN DATA OF INFLAMMATORY RESPONSE TO THE BASE THERAPY OF THE JUVENILE IDIOPATHIC ARTHRITIS","authors":"Y. Vyzhga","doi":"10.21303/2585-663.2018.00762","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00762","url":null,"abstract":"The activity of the juvenile idiopathic arthritis evaluates the speed of the process progression, intense of its clinical manifestation, effect of the carried treatment. The aim of our study was to estimate changes of the inflammatory response signs as well as content of NF-kB due to basic therapy of the JIA. Materials and methods. At our study, we had checked 68 children with JIA, who passed their treatment at Vinnytsya regional children’s hospital within the period from 2011 to 2014 years. Results. At the patients we studied currency of the JIA was characterized with articular variant of the disease, mainly in monoarthritis type. Laboratory activity of the inflammatory response characterized with increased content of the C-reactive protein (71.2±3.7 %), inflammatory cytokines – ІL-1β (54.8±4.1 %) and ІL-6 (56.2±2.4 %), as well high quantity of the nuclear factor-kB (70.5±3.1 %). Currency of the JIA in children characterized with high increasing of the inflammatory response signs especially C-reactive protein (6.55(4.2;9.8)), ІL-1β (7.3(3.5;11.9)), ІL-6 (6.8(4.5;10.6)) and NF-kB (6.76 (4.8; 9.1)), that are in correlative connections with clinical signs (number of the injured and swelled joints, evaluation of the general condition of the child according to doctors and own response) of the disease activity (rxy = +0.34 up to 0.62, p< 0.01). Conclusion. During the managing of the basic therapy in children with JIA we estimated decreasing of the IL-1β content in patients at the background of methotrexate administration (38.7±3.7 %), at the second group with use of sulfasalazine (28.5±3.5 %) and the third with leflunomide prescription (29.1±5.1 %), but significant decreasing of the ІL-6 content, that is one of the main inflammatory mediators and as well NF-kB was found just in group of the patients with methotrexate administration (on 36.3±3.8 % and 32.4±2.4 % for NF-kB).","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"116 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77274937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00751
O. Lys
Nowadays, one of the most important problems are stressful reaction of human and animal systems. The purpose of our study was to find out the peculiarities of the dynamics of changes in the indices of diene conjugates and malonic dialdehyde in blood in rats under the conditions of development of immobilization stress. Material and methods of research. The experiments were carried out on white male rats of the Vistar line weighing 180–200 g which were divided into 3 groups for 10 animals (one control and three tested). The control group of rats was injected with 1 mg physiological saline per kg body weight intraperitoneally. The animals were decapitated under anes the siaca used by sodium thiopental (intraperitoneal injection of 1 % solution of 50 mg / kg) and withdrawn from the experiment after 1, 3, and 5 days. In blood plasma was determined the content of diene conjugates and malonic dialdehyde in stress-induced rats, the groups were compared with the control group rats. Results. The state of the processes (POFs) was assessed by the content of blood vessels detected in different periods (1 st, 3 rd, and 5 th day) after conducting of IS in rats. Under the conditions of the development of the IS, an increase in the content of DK was observed at 116.96 % (p<0.05) in the experimental group1 accordingly on the 1st day after the IS compared with the control. For the 3rd day of the study, the level of DK increased by 44.24 % (p<0.05) in experimental group 2, against the intact group of animals. Experimental group 3, in which animals were withdrawn from the IS for the 5th day, was characterized by an increase in the content of DK by 41.84 % (p<0.05) compared with the control group. Another indicator of MDA in the blood for 1 day of the experiment in experimental group 1 increased by 104.15 % (p<0.05) in the case of IS in relation to the intact group. On the 3rd day of the experiment under the conditions of development of IP, the level of MDA increased by 83.9 % (p<0.05), against control. At the 5th day of the experiment, MDA level was 30.1 % (p<0.05) for IS, when compared with control Conclusion. Consequently, the experiment made it possible to establish the significant activation of the LP processes, which we determined with increasing levels of DC and MDA in animals under IS conditions, the most of it was on the 1th day of the experiment.
{"title":"CONTENT OF DIENE KOH'UGATIVES AND MALONIC DIALDEHYDE IN BLOOD FOR RATS IN DYNAMICS OF FORMATION OF IMMOBILIZATIONAL STRESS","authors":"O. Lys","doi":"10.21303/2585-663.2018.00751","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00751","url":null,"abstract":"Nowadays, one of the most important problems are stressful reaction of human and animal systems. The purpose of our study was to find out the peculiarities of the dynamics of changes in the indices of diene conjugates and malonic dialdehyde in blood in rats under the conditions of development of immobilization stress. Material and methods of research. The experiments were carried out on white male rats of the Vistar line weighing 180–200 g which were divided into 3 groups for 10 animals (one control and three tested). The control group of rats was injected with 1 mg physiological saline per kg body weight intraperitoneally. The animals were decapitated under anes the siaca used by sodium thiopental (intraperitoneal injection of 1 % solution of 50 mg / kg) and withdrawn from the experiment after 1, 3, and 5 days. In blood plasma was determined the content of diene conjugates and malonic dialdehyde in stress-induced rats, the groups were compared with the control group rats. Results. The state of the processes (POFs) was assessed by the content of blood vessels detected in different periods (1 st, 3 rd, and 5 th day) after conducting of IS in rats. Under the conditions of the development of the IS, an increase in the content of DK was observed at 116.96 % (p<0.05) in the experimental group1 accordingly on the 1st day after the IS compared with the control. For the 3rd day of the study, the level of DK increased by 44.24 % (p<0.05) in experimental group 2, against the intact group of animals. Experimental group 3, in which animals were withdrawn from the IS for the 5th day, was characterized by an increase in the content of DK by 41.84 % (p<0.05) compared with the control group. Another indicator of MDA in the blood for 1 day of the experiment in experimental group 1 increased by 104.15 % (p<0.05) in the case of IS in relation to the intact group. On the 3rd day of the experiment under the conditions of development of IP, the level of MDA increased by 83.9 % (p<0.05), against control. At the 5th day of the experiment, MDA level was 30.1 % (p<0.05) for IS, when compared with control Conclusion. Consequently, the experiment made it possible to establish the significant activation of the LP processes, which we determined with increasing levels of DC and MDA in animals under IS conditions, the most of it was on the 1th day of the experiment.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89668217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00763
A. Antonenko, O. Vavrinevych
The chemical plant protection products application is an integral part of intensive crop production technologies. However, scientists have proven that the increase in chemical pollution of the environment in 2 times increases the general level of morbidity of the adult population by 25%. That is why the risk assessment of pesticide-contaminated products consumption is an important part of the regulatory process. Methods. The parameters of fungicides difenoconazole, tebuconazole, cyflufenamid, oxathiapiprolin, famoxadone persistence in agricultural crops were studied in the field experiments. For the integrated assessment of the potential risk of pesticide-contaminated products consumption for human (RPCPC), a methodology developed on the basis of the Institute of Hygiene and Ecology was used. The acceptable daily intake (ADI), the half-life period (τ50) in plants and the average daily consumption of the product have been evaluated using four-graded scale. Results. According to the persistency in tomatoes and potatoes, fungicides difenoconazole, cyflufenamid, famoxadone are pertained to 3 classes of hazard (5-14 days); tebuconazole - to 2 class (15-30 days); oxathiapiprolin - to 4 class (less than 5 days). After the addition of all the obtained points, the RPCPC value (tomatoes and potatoes) for difenoconazole, cyflufenamid, famoxadone amounted 8 points (2+2+2× 2 and 2+2+4, respectively); tebuconazole - 8 points (3+1+2×2 or 3+1+4, respectively); oxathiapiprolin - 6 points (1+1+2×2 or 1+1+4, respectively). Discussion. Thus, the studied compounds belong to the 3rd class of hazard according to pesticide-contaminated products consumption for human index value. The results obtained by us correlate with the studies carried out in the previous stage. The exception was only difenoconazole, which was classified as hazard class 2 due to its high toxicity for human.
{"title":"FORECASTING OF TRIAZOLE, AMIDE, PIPEREDINYLE THIAZOL ISOXAZOLINE, OXAZOLE FUNGICIDES HAZARDOUS EFFECT ON HUMAN HEALTH IN CONSUMPTION OF VEGETABLES GROWED IN THEIR APPLICATION","authors":"A. Antonenko, O. Vavrinevych","doi":"10.21303/2585-663.2018.00763","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00763","url":null,"abstract":"The chemical plant protection products application is an integral part of intensive crop production technologies. However, scientists have proven that the increase in chemical pollution of the environment in 2 times increases the general level of morbidity of the adult population by 25%. That is why the risk assessment of pesticide-contaminated products consumption is an important part of the regulatory process. Methods. The parameters of fungicides difenoconazole, tebuconazole, cyflufenamid, oxathiapiprolin, famoxadone persistence in agricultural crops were studied in the field experiments. For the integrated assessment of the potential risk of pesticide-contaminated products consumption for human (RPCPC), a methodology developed on the basis of the Institute of Hygiene and Ecology was used. The acceptable daily intake (ADI), the half-life period (τ50) in plants and the average daily consumption of the product have been evaluated using four-graded scale. Results. According to the persistency in tomatoes and potatoes, fungicides difenoconazole, cyflufenamid, famoxadone are pertained to 3 classes of hazard (5-14 days); tebuconazole - to 2 class (15-30 days); oxathiapiprolin - to 4 class (less than 5 days). After the addition of all the obtained points, the RPCPC value (tomatoes and potatoes) for difenoconazole, cyflufenamid, famoxadone amounted 8 points (2+2+2× 2 and 2+2+4, respectively); tebuconazole - 8 points (3+1+2×2 or 3+1+4, respectively); oxathiapiprolin - 6 points (1+1+2×2 or 1+1+4, respectively). Discussion. Thus, the studied compounds belong to the 3rd class of hazard according to pesticide-contaminated products consumption for human index value. The results obtained by us correlate with the studies carried out in the previous stage. The exception was only difenoconazole, which was classified as hazard class 2 due to its high toxicity for human.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87904577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00772
A. Pavliak
The mortality from surgical sepsis remains high and ranges from 17 to 83.7 %. The cause of high lethality is endogenous intoxication and multiple organ failure. The most common laboratory markers for determining the syndrome of endogenous intoxication in surgical sepsis are leukocyte and hematological intoxication indices, such as LII, HII, NII. Aim of the work: to study the predictive value of laboratory and hematological indicators of intoxication in the diagnosis of surgical sepsis. Materials and methods. A retrospective analysis of disease history and prospective follow-up of 41 patients treated for surgical sepsis in the Ivano-Frankivsk (Ukraine) Surgical Department of the CCCH in period between August 2015 and August 2018 was conducted. In the first day after the operation, all the patients been calculated the leukocyte index of intoxication, the hematological index of intoxication and the nuclear index of intoxication to assess their predictive and diagnostic significance. Methods of nonparametric statistics were used in the study: ROC-analysis, Mann-Whitney U-test, Spierman's criterion (Rs). Results. The results of the diagnostic and prognostic significance of LII, HII, NII for 1 day postoperative period, with regard to the prediction of lethality by means of ROC analysis, Mann-Whitney U-test, Spirman criteria (Rs) showed low sensitivity and specificity in patients with surgical sepsis. Common laboratory leukocyte and hematological non-specific indicators of endogenous intoxication determination have low prognostic and diagnostic significance in assessing the degree of endotoxicosis in surgical sepsis and can not serve as prognostic criteria for lethality. Conclusions. The problem of diagnosing endotoxicosis in surgical sepsis requires the further study and introduction into clinical practice of new specific and integrated clinical and laboratory methods for the quantitative determination of endotoxicosis, which could serve as a reliable diagnostic criterion in assessing the severity of the condition and the prediction of hospital mortality.
{"title":"ANALYSIS OF PROGNOSTIC VALUE OF LEUKOCYTE AND HEMATOLOGICAL INDEXES OF INTOXICATION IN THE DIAGNOSIS OF SURGICAL SEPSIS","authors":"A. Pavliak","doi":"10.21303/2585-663.2018.00772","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00772","url":null,"abstract":"The mortality from surgical sepsis remains high and ranges from 17 to 83.7 %. The cause of high lethality is endogenous intoxication and multiple organ failure. The most common laboratory markers for determining the syndrome of endogenous intoxication in surgical sepsis are leukocyte and hematological intoxication indices, such as LII, HII, NII. Aim of the work: to study the predictive value of laboratory and hematological indicators of intoxication in the diagnosis of surgical sepsis. Materials and methods. A retrospective analysis of disease history and prospective follow-up of 41 patients treated for surgical sepsis in the Ivano-Frankivsk (Ukraine) Surgical Department of the CCCH in period between August 2015 and August 2018 was conducted. In the first day after the operation, all the patients been calculated the leukocyte index of intoxication, the hematological index of intoxication and the nuclear index of intoxication to assess their predictive and diagnostic significance. Methods of nonparametric statistics were used in the study: ROC-analysis, Mann-Whitney U-test, Spierman's criterion (Rs). Results. The results of the diagnostic and prognostic significance of LII, HII, NII for 1 day postoperative period, with regard to the prediction of lethality by means of ROC analysis, Mann-Whitney U-test, Spirman criteria (Rs) showed low sensitivity and specificity in patients with surgical sepsis. Common laboratory leukocyte and hematological non-specific indicators of endogenous intoxication determination have low prognostic and diagnostic significance in assessing the degree of endotoxicosis in surgical sepsis and can not serve as prognostic criteria for lethality. Conclusions. The problem of diagnosing endotoxicosis in surgical sepsis requires the further study and introduction into clinical practice of new specific and integrated clinical and laboratory methods for the quantitative determination of endotoxicosis, which could serve as a reliable diagnostic criterion in assessing the severity of the condition and the prediction of hospital mortality.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83595340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-21DOI: 10.21303/2585-663.2018.00739
T. Yarnykh, O. Rukhmakova
The aim of this work was the development of technology of rectal suppositories of immunomodulatory action for children and their standardization. To obtain suppositories, a licorice root extract, chamomile and tea tree essential oils were used. Given that the introduction of ingredients in a dissolved form is preferable, we studied the possibility of using emulsion suppository bases. To evaluate the compositions, the homogeneity and colloidal stability of the suppositories obtained were studied. Estimation of homogeneity, melting temperature, decay time, average mass was carried out according to the State Pharmacopoeia. To confirm the authenticity of the active ingredients, a chromatography method was used. The quantitative determination of glycyrrhizin acid was carried out by spectrophotometric method. Suppositories were prepared by the pouring method. The licorice root extract was added to the suppository when dissolved in the hydrophilic phase of the base. Essential oils of chamomile and tea tree were introduced when a solid fat of type A dissolved in a melt, at a temperature of (42.0±2.0) ºC. Optimum indices for the criteria of “homogeneity” and “colloidal stability” were noted for the samples of the composition: purified water, polysorbate-80, lecithin, solid fat type A. To prove the authenticity of the licorice root extract in suppositories, the chloroform-methanol-water system was optimal (26:14:3). Identification of essential oils on a gas chromatograph showed that on the chromatogram the peaks and retention times of the solution under study coincide with the peaks and retention times of the reference solutions. The conducted studies of quantitative determination of glycyrrhizin acid have shown that its content in one suppository is not less than 0.035 g in terms of glycyram.
{"title":"THE ASPECTS OF DEVELOPMENT AND STANDARDIZATION OF CHILDREN’S SUPPOSITORIES WITH EXTRACT OF LICORICE ROOT","authors":"T. Yarnykh, O. Rukhmakova","doi":"10.21303/2585-663.2018.00739","DOIUrl":"https://doi.org/10.21303/2585-663.2018.00739","url":null,"abstract":"The aim of this work was the development of technology of rectal suppositories of immunomodulatory action for children and their standardization. To obtain suppositories, a licorice root extract, chamomile and tea tree essential oils were used. Given that the introduction of ingredients in a dissolved form is preferable, we studied the possibility of using emulsion suppository bases. To evaluate the compositions, the homogeneity and colloidal stability of the suppositories obtained were studied. Estimation of homogeneity, melting temperature, decay time, average mass was carried out according to the State Pharmacopoeia. To confirm the authenticity of the active ingredients, a chromatography method was used. The quantitative determination of glycyrrhizin acid was carried out by spectrophotometric method. Suppositories were prepared by the pouring method. The licorice root extract was added to the suppository when dissolved in the hydrophilic phase of the base. Essential oils of chamomile and tea tree were introduced when a solid fat of type A dissolved in a melt, at a temperature of (42.0±2.0) ºC. Optimum indices for the criteria of “homogeneity” and “colloidal stability” were noted for the samples of the composition: purified water, polysorbate-80, lecithin, solid fat type A. To prove the authenticity of the licorice root extract in suppositories, the chloroform-methanol-water system was optimal (26:14:3). Identification of essential oils on a gas chromatograph showed that on the chromatogram the peaks and retention times of the solution under study coincide with the peaks and retention times of the reference solutions. The conducted studies of quantitative determination of glycyrrhizin acid have shown that its content in one suppository is not less than 0.035 g in terms of glycyram.","PeriodicalId":33846,"journal":{"name":"Technology Transfer Innovative Solutions in Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82316307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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