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Foraging behaviour alterations of Culex quinquefasciatus Say (Diptera: Culicidae) mosquito larvae induced by Romanomermis iyengari Welch (Nematoda: Mermithidae) parasitism 致倦库蚊(双翅目:库蚊科)幼虫寄生对其觅食行为的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220301085416
H. Elbrense, A. Seif, Ibrahem B. Helal, Mohamed M. Shamseldean, Iman Elhussieny
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引用次数: 0
Genes controlling appetite, gut emptying rate and metabolism in the early stage of Rotenone-induced semi-Parkinsonism in Drosophila melanogaster 鱼藤酮诱导的黑腹果蝇半帕金森病早期控制食欲、肠道排空率和代谢的基因
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220811020747
Mai Fathy, Mohamed Shaheen, S. Kholy
Parkinson’s disease (PD) is one of the most serious neurodegenerative diseases. PD is characterized by progressive death of dopamine producing cells in Substantia nigra pars compacta within human brain causing serious locomotor defects such as tremor as well as non-motor symptoms including metabolic deficiency and constipation. Despite the plethora of information regarding the underlying mechanisms related to locomotion impairment, signal pathways/genes involved in metabolic deficiency and constipation remain unclear. This study aims to explore the most important key genes involved in metabolic syndrome and constipation observed in PD patients. To achieve this goal, real-time polymerase chain reaction (RT PCR) of a group of genes supposed in previous findings to have a role in these syndromes in rotenone - induced PD Drosophila model was performed. One -day- old Canton S flies were fed on filter paper saturated with rotenone glucose solution(5mM rotenone in 10% glucose) at 25 ̊C for 24h.Thereafter, we focus on genes whose up-regulated expression level for all subsequent experiment. Next, metabolic parameters (total protein, lipid and carbohydrates), rate of food consumption and defecation in flies that’s knocking down in the corresponding genes were evaluated. Results revealed that feeding rate decreased when CG17544 and CG16848 genes were up-regulated; consequently those flies had reduced body weight. Meanwhile, knocking down CG16848 and CG12913. This study provide evidence that using the human homologues of these Drosophila genes for molecular docking of new pharmacotherapeutics may help to enhance the quality of life of PD patients through improving metabolism as well as defecation.
帕金森病(PD)是最严重的神经退行性疾病之一。PD的特点是人脑致密黑质产生多巴胺的细胞进行性死亡,导致严重的运动缺陷,如震颤,以及代谢不足和便秘等非运动症状。尽管关于运动障碍相关的潜在机制的信息过多,但代谢缺乏和便秘相关的信号通路/基因仍不清楚。本研究旨在探讨PD患者代谢综合征和便秘最重要的关键基因。为了实现这一目标,我们在鱼藤酮诱导的帕金森病果蝇模型中对一组先前研究中被认为在这些综合征中起作用的基因进行了实时聚合酶链反应(RT PCR)。取1日龄广东蝇,用鱼藤酮葡萄糖溶液(10%葡萄糖中含有5mM鱼藤酮)饱和滤纸在25℃下饲养24h。此后,我们在后续的所有实验中都将重点关注表达水平上调的基因。接下来,研究人员评估了果蝇的代谢参数(总蛋白质、脂质和碳水化合物)、食物消耗率和排便率,这些参数都在相应的基因中被敲除。结果表明,上调CG17544和CG16848基因,摄食率降低;因此,这些苍蝇的体重减轻了。同时击倒CG16848和CG12913。本研究为利用这些果蝇基因的人类同源物进行新药物治疗的分子对接提供了证据,可能有助于通过改善代谢和排便来提高PD患者的生活质量。
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引用次数: 0
Histopathological Studies on Experimentally Eimeria tenella-Infected Broiler Chicken 嫩艾美耳球虫实验性感染肉鸡的组织病理学研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190810095333
I. Helal, S. Ahmed, S. Amer
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引用次数: 3
Description of two novel Echinochasmus species (Digenea: Echinostomatidae) from the squacco heron Ardeola ralloides (Aves: Ardeidae) in Egypt: A light and scanning electron microscopic study 埃及沙苍鹭Ardeola ralloides(鸟类:沙苍鹭科)两种棘孔虫新种的描述:光镜和扫描电镜研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190908034421
R. Mansour, Amina Elfatah
Adult forms of two new echinostomatids were recovered from small intestine of six out of ten squacco herons, Ardeola ralloides collected from March to July 2019 from Giza governorate, Egypt. These small-sized parasites had a single row of collar spines with middle dorsal interruption, vitellaria reaching the level of ventral sucker and attributed to genus Echinochasmus, namely, E. ralloides n. sp. (prevalence 40%) and E. rawashi n. sp. (prevalence 20%). The morphometric features and differential indices of two species were measured, compared and described using light and scanning electron microscopy (SEM). The body of E. ralloides n. sp. measured 959.2-1090.6 × 264.3-311.1 μm (L × MW). It was provided with 26 collar spines (9 dorsal, 2 lateral and 2 angle on each side of the dorsal collar interruption), lacked of tegumental spines except for small areas in lower left and lateral right margins of ventral sucker, and characterized by presence of many minute pores of 3.1-3.8 μm on dorsal surface of hindbody. The body of E. rawashi n. sp. measured 638.8-1173.5 × 138.8-207.7 μm (L × MW), provided with 24 collar spines (7 dorsal, 2 lateral and 2 angle on each side of the dorsal collar interruption) and had scale-like tegumental spines distributed on whole ventral surface and anterior 1/4 of dorsal surface of body, ended by up to 13 digit-like structures and measured 2.1-2.5 × 4.4-4.8 μm.
2019年3月至7月在埃及吉萨省收集的10只沙鹭中有6只的小肠中发现了两种新的棘孔虫成虫。这些小型寄生虫具有单排颈棘,中间背侧断裂,卵黄达到腹吸盘水平,属于棘球绦虫属,即E. ralloides n. sp.(患病率40%)和E. rawashi n. sp.(患病率20%)。利用光镜和扫描电镜对两种植物的形态特征和差异指标进行了测量、比较和描述。E. ralloides n.sp .的体长为959.2 ~ 1090.6 × 264.3 ~ 311.1 μm (L × MW)。体表有26根颈刺(颈突背向9根,颈突侧面2根,颈突两侧各2根),除腹侧吸盘左下缘和右外侧缘小面积外,无被盖棘,后体背表面有许多3.1 ~ 3.8 μm的微孔。rawwashi n. sp.体长为638.8-1173.5 × 138.8-207.7 μm (L × MW),体长24个领刺(背领中断两侧各有7个背向,2个侧向和2个角度),整个腹面和前1/4背面分布有鳞片状被棘,末端有多达13个趾状结构,体长为2.1-2.5 × 4.4-4.8 μm。
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引用次数: 1
Description of a novel aseptate gregarine Zygocystis Stein, 1848 (Apicomplexa: Monocystinae) from earthworms (Annelida: Oligochaeta) of Egypt 埃及蚯蚓(环节动物:寡毛纲)中一种新分离的双胞虫(Zygocystis Stein, 1848)的描述
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190810085854
N. Ramadan, Samia Fawzy, Medhat Ali
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引用次数: 0
Low- versus High-water level effect on subtropical benthic freshwater meiofauna 低水位与高水位对亚热带底栖淡水小型动物的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20221229092731
Ahmed Abada
Little is known about meiofaunal responses to Low- versus High-Water-Level (L- vs HWL) particularly in the subtropics. Meiofaunal reaction was evaluated in three locations (deeply-different) in two man-made freshwater channels in (Rowaina and Shalma) Northern Nile Egyptian delta: 50, 100, & 150, and 900, 950, & 1000 metres distances from the sluice, respectively. LWL and HWL were in January and July 2019 respectively. Eight vs ten and nine vs eleven major taxa were noted in Rowaina H- vs LWL and Shalma L- vs HWL, respectively. Taxa had different susceptibilities to L- vs HWL. Nematode, hydracarina, and oligochaetes dominated in L- and HWL, (Shalma), and in HWL (Rowaina). Whereas Nematoda, Copepod, and Oligochaeta prevailed in LWL (Rowaina). Oligochaeta biomass was generally high in LWL and HWL. Hydracarina in LWL, and Nematode & Copepod in HWL had low biomasses in Rowaina and Shalma respectively. Abundances and biomasses were significantly different within and among channels. Environmental variables were significantly different only between water levels. It could be concluded that L- vs HWL has both direct and indirect (variabilities in environmental factors) impacts on meiofauna. Channel width & morphology, may impose extra influences. This study may enhance invertebrate surveys with hydrologically different freshwater bodies.
人们对低水位和高水位(L- vs HWL)的微生态反应知之甚少,特别是在亚热带地区。在北尼罗河埃及三角洲(Rowaina和Shalma)的两个人工淡水通道的三个地点(差异很大)评估了Meiofaunal反应:分别距离水闸50、100和150米,以及900、950和1000米。LWL和HWL分别为2019年1月和7月。罗维纳L- vs LWL和沙尔马L- vs HWL的主要分类群分别为8 vs 10和9 vs 11。不同分类群对L-和HWL的敏感性不同。线虫、水螅虫和寡毛纲在L-和HWL (sharma)以及HWL (Rowaina)中占主导地位。而线虫、桡足类和寡毛纲在罗维纳省占优势。低陆和高陆寡毛藻生物量普遍较高。LWL的水螅虫和HWL的线虫和桡足类动物分别在罗威纳和沙马的生物量较低。通道内和通道间丰度和生物量差异显著。环境变量仅在不同水位之间存在显著差异。由此可见,L- vs . HWL对小型动物有直接和间接(环境因子变异)影响。通道宽度和形态,可能会施加额外的影响。该研究可加强不同水文淡水水体的无脊椎动物调查。
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引用次数: 0
Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA 氧化锌纳米颗粒对新生和成年大鼠小脑的影响及EDTA可能的保护作用的组织学和免疫组化研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20221222014955
Siham B. Salem, S. Naggar, Ramadan M. Kandyel, M. Sharkawy
Zinc oxide nanoparticles (ZnO NPs) are involved in different applications such as nutrition, while, it causes damage to different organs of the body including the brain. This study aimed to compare between the effect of ZnO NPs on newborn and adult rats´ cerebellum and to evaluate the protective role of Ethylenediaminetetraacetic acid (EDTA) against ZnO NPs toxicity. Thirty-two newborn (NBM)and thirty-two adult male (ADM) Sprague Dawley rats are used. Four groups (n = 8) of each are divided as following; Gp1: is served as control, Gp2: injected intraperitoneal (IP) with ZnO NPs (3 g/ kg). Gp3: injected IP with EDTA (500 mg/ kg) for 7 days. Gp4: injected with ZnO NPs and treated with EDTA as in Gp3. Brain tissue is prepared for biochemical and histopathological investigations. Newborn and adult rats injected with ZnO NPs showed degeneration of purkinje and granular cells in cerebellum tissue confirmed with high expression of caspase-3, while, treatment with EDTA post ZnO NPs showed lightly improvement. The body weights and relative brain weights is decrease in all the rats treated with ZnO NPs. In addition, our current study proved that the effect of ZnO NPs on ADM rats is more than on the NBM ones. Collectively, ZnO NPs injection caused damage of the cerebellum in newborn and adult rats, however, the treatment with EDTA stopped somewhat this damage.
氧化锌纳米粒子(ZnO NPs)在营养等方面有着广泛的应用,同时也会对包括大脑在内的人体不同器官造成损害。本研究旨在比较氧化锌NPs对新生和成年大鼠小脑的影响,并评价乙二胺四乙酸(EDTA)对氧化锌NPs毒性的保护作用。采用32只新生大鼠(NBM)和32只成年雄性大鼠(ADM)。每组四组(n = 8)分为如下:Gp1:作为对照组,Gp2:腹腔注射ZnO NPs (3 g/ kg)。Gp3:给IP注射EDTA (500 mg/ kg),持续7天。Gp4:与Gp3一样,注入ZnO NPs并经EDTA处理。脑组织准备用于生化和组织病理学检查。新生大鼠和成年大鼠注射氧化锌NPs后,浦肯野细胞变性,小脑颗粒细胞高表达caspase-3,而氧化锌NPs后EDTA处理则有轻微改善。氧化锌NPs处理后大鼠的体重和相对脑重量均有所下降。此外,我们目前的研究证明ZnO NPs对ADM大鼠的影响大于对NBM大鼠的影响。总的来说,ZnO NPs注射引起新生和成年大鼠小脑损伤,但EDTA治疗在一定程度上阻止了这种损伤。
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引用次数: 0
RNA/DNA ratio and expression of GHR and IGF-1 hormones: A study on seasonal variation effect on growth characterization of male Oreochromis niloticus RNA/DNA比值及GHR和IGF-1激素的表达:季节变化对雄性尼罗鱼生长特性的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190715025948
I. Garawani, E. Khallaf, Alla A. Alne na ei, R. G. Elgendy
Nile tilapia (Oreochromis niloticus) is considered as one of the most economical and popular aquaculture species. One of the main concerns in tilapia aquaculture, as well as in the wild, is growth, because of its importance for the fish population, stock assessment and their production in aquaculture. Accordingly, this study was initiated to characterize growth on molecular basis. In the same respect, RNA/DNA ratio and GHR-1/IGF-1as growth-related genes are known to be involved in weight gain alterations. To assess the correlation between growth, biochemical and hormonal conditions of fish during summer and winter. Adult male O. niloticus (100 - 250gm) were collected from Bahr Shebeen Canal, for such investigation. The results revealed that the GHR-1 and IGF-1 mRNA were directly proportional to age, length and weight. However, they were up regulated in summer than in winter. However, RNA/DNA ratio gave an observable positive correlation with age and length only with higher values in winter. In conclusion, this study supports the use of GHR-1/IGF-1as reliable indices of growth and condition of O. niloticus in their natural habitat in addition to RNA/DNA ratio assessment.
尼罗罗非鱼(Oreochromis niloticus)被认为是最经济和最受欢迎的水产养殖品种之一。罗非鱼水产养殖以及野生罗非鱼的主要问题之一是生长,因为它对鱼类种群、种群评估及其水产养殖生产具有重要意义。因此,本研究开始在分子基础上表征生长。同样,已知RNA/DNA比率和GHR-1/ igf -1生长相关基因与体重增加的改变有关。评估鱼类在夏季和冬季的生长、生化和激素状况之间的相关性。在巴尔谢比运河采集成蚊雄蚊(100 ~ 250克)进行调查。结果显示,GHR-1和IGF-1 mRNA与年龄、身高和体重成正比。然而,它们在夏季比冬季受到更高的管制。RNA/DNA比值与年龄和体长呈显著正相关,且在冬季较高。综上所述,本研究支持除RNA/DNA比值评估外,GHR-1/ igf -1作为niloticus自然生境生长状况的可靠指标。
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引用次数: 0
Rationalized manner for controlling Sitophilus oryzae (L.) and Rhyzopertha dominica (F.) using spinosad and ivermectin biocides. 利用spinosad和伊维菌素杀菌剂防治米象和米曲蝇的合理方法。
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190708120158
T. Ismail, Mostafa E. E. Elebiary
In this study, we evaluated the efficiency of two biocides, spinosad and ivermectin against two important stored grain insects, Sitophilus oryzae (L.) and Rhyzopertha dominica (F.) by using bioassay method, mixing with medium. Obtained results demonstrated that ivermectin had the highest effect on adult mortality for both insects using complete dose. Rates of spinosad or ivermectin from 50-150 ppm completely prevented emergence of adults of both insects. R. dominica was more susceptible to biocides tested than S. oryzae. Half-dose of spinosad or ivermectin nearly produced the same effect with complete dose on S. oryzae and R. dominica, while the one-third dose was less effect. The current results encourage using the half-dose instead of the full dose of both insecticides for controlling the tested insects to rationalize the insecticide application and to minimize their expected hazards.
本研究采用生物测定法,与培养基混合,评价了两种杀菌剂spinosad和伊维菌素对两种重要储粮昆虫米象(Sitophilus oryzae, L.)和米纹虫(Rhyzopertha dominica, F.)的杀虫效果。结果表明,伊维菌素在完全剂量下对两种昆虫的成虫死亡率影响最大。50- 150ppm的spinosad或伊维菌素完全阻止了这两种昆虫成虫的出现。多米尼克菌对杀菌剂的敏感性高于稻瘟病菌。一半剂量的spinosad或伊维菌素对米曲菌和多米尼加弧菌的效果与完全剂量几乎相同,而三分之一剂量的效果较差。目前的研究结果鼓励使用两种杀虫剂的半剂量而不是全剂量来控制受试昆虫,以使杀虫剂的施用合理化并将其预期危害降到最低。
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引用次数: 1
Role of milk thistle (Silybum marianum) oil in the improvement of carbon tetrachloride-induced liver fibrosis and cirrhosis in albino mice 水飞蓟油在改善四氯化碳诱导的白化小鼠肝纤维化和肝硬化中的作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220404091009
Nabila Desouki, Basyouny Elfotouh, Soha Okba, Buthina Alshammari
{"title":"Role of milk thistle (Silybum marianum) oil in the improvement of carbon tetrachloride-induced liver fibrosis and cirrhosis in albino mice","authors":"Nabila Desouki, Basyouny Elfotouh, Soha Okba, Buthina Alshammari","doi":"10.5455/egysebz.20220404091009","DOIUrl":"https://doi.org/10.5455/egysebz.20220404091009","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133001217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)
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