Pub Date : 1900-01-01DOI: 10.5455/egysebz.20190801083542
N. Ibrahim, Elsie Rizk, Ibrahim Shourbagy, Dina Shammari
This study planned to assess the toxicity, biochemical investigation associated with hepatotoxicity and nephrotoxicity of insecticide Imidacloprid (Commando 30%) in addition to serum cholinesterase (chE) activity in albino mice at orally administered sublethal dose of Commando 1/20 of LD50. Results revealed that The LD50 values obtained at 24, 48, 72 and 96 hours exposures and the 95% confidence limits for the imidacloprid (Commando 30%) obtained from the sigmoid curve were 495, 437, 344 and 210 mg/kg bw, respectively. the net body weight gains of the animals intoxicated with sublethal doses of Commando 30% was markedly significant decrease (P≤ 0.5). Significant reduction (P ˂ 0.05) in total protein, and significant increase in ALT, AST, ALP and LDH enzyme activity levels as well as significant decrease in kidney function (urea and creatinine) in all the experimental groups of mice. Serum Cholinestrase (ChE) activity was markedly significant decrease by Commando exposure mice at sublethal dose. The blood parameters of all tested groups, results showed that the median values of blood parameters decreased in animals treated with Commando 30% at a dose of 10.5 mg/kg body weight, indicative of anemic syndrome. In addition, there was a significant reduction in the number of erythrocytes and of hemoglobin concentration, with reduced hematocrit and increased MCV, characteristic of macrocytic anemia.
{"title":"Biochemical changes of the Liver and Kidney of Albino Mice Mus musculus Exposed to Organphosphorus insecticide Imidacloprid (Commando 30%)","authors":"N. Ibrahim, Elsie Rizk, Ibrahim Shourbagy, Dina Shammari","doi":"10.5455/egysebz.20190801083542","DOIUrl":"https://doi.org/10.5455/egysebz.20190801083542","url":null,"abstract":"This study planned to assess the toxicity, biochemical investigation associated with hepatotoxicity and nephrotoxicity of insecticide Imidacloprid (Commando 30%) in addition to serum cholinesterase (chE) activity in albino mice at orally administered sublethal dose of Commando 1/20 of LD50. Results revealed that The LD50 values obtained at 24, 48, 72 and 96 hours exposures and the 95% confidence limits for the imidacloprid (Commando 30%) obtained from the sigmoid curve were 495, 437, 344 and 210 mg/kg bw, respectively. the net body weight gains of the animals intoxicated with sublethal doses of Commando 30% was markedly significant decrease (P≤ 0.5). Significant reduction (P ˂ 0.05) in total protein, and significant increase in ALT, AST, ALP and LDH enzyme activity levels as well as significant decrease in kidney function (urea and creatinine) in all the experimental groups of mice. Serum Cholinestrase (ChE) activity was markedly significant decrease by Commando exposure mice at sublethal dose. The blood parameters of all tested groups, results showed that the median values of blood parameters decreased in animals treated with Commando 30% at a dose of 10.5 mg/kg body weight, indicative of anemic syndrome. In addition, there was a significant reduction in the number of erythrocytes and of hemoglobin concentration, with reduced hematocrit and increased MCV, characteristic of macrocytic anemia.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130655452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/egysebz.20220907064204
Z. Attia, Hala Abdelazem, Iman Nada
The effect of hypoxia and reoxygenation on Nile tilapia (Oreochromis niloticus) was studied. The fish were exposed to hypoxic oxygen concentrations (2 and 0.5 mg O2 L-1) for 1 day (short term) and 30 days (long term) followed by reoxygenation for 1 day and 7 days, respectively. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) significantly decreased in the gills of all the hypoxic groups after both short and long term of hypoxia exposures, while increased significantly below control levels after reoxygenation except that the activities of SOD and GST in the reoxygenated groups pre-exposed to short-term (2 mg O2 L-1), SOD activity in the reoxygenated group pre-exposed to long-term (0.5 mg O2 L-1), and the activities of CAT and GST in all the reoxygenated groups pre-exposed to long-term hypoxia, had no significant change compared to their respective hypoxic groups. The activities of glutathione peroxidase (GPx), glutathione reductase (GR) and xanthine oxidase (XO) significantly increased during hypoxia then decreased significantly after reoxygenation except for GPx activity in the reoxygenated group pre-exposed to short-term (2 mg O2 L-1). Malondialdehyde (MDA) concentrations significantly increased in all the hypoxic and reoxygenated groups compared to their respective controls. The significance of these alterations in enzyme activities and oxidative stress indices is discussed. In conclusion, hypoxia and subsequent reoxygenation induce oxidative stress in Oreochromis niloticus gills causing depletion of some antioxidant enzymes. Long-lasting oxidative stress can delay full recovery.
{"title":"Effect of hypoxia and reoxygenation on antioxidant system in gills\u0000of tilapia fish (Oreochromis niloticus)","authors":"Z. Attia, Hala Abdelazem, Iman Nada","doi":"10.5455/egysebz.20220907064204","DOIUrl":"https://doi.org/10.5455/egysebz.20220907064204","url":null,"abstract":"The effect of hypoxia and reoxygenation on Nile tilapia (Oreochromis niloticus) was studied. The fish were exposed to hypoxic oxygen concentrations (2 and 0.5 mg O2 L-1) for 1 day (short term) and 30 days (long term) followed by reoxygenation for 1 day and 7 days, respectively. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) significantly decreased in the gills of all the hypoxic groups after both short and long term of hypoxia exposures, while increased significantly below control levels after reoxygenation except that the activities of SOD and GST in the reoxygenated groups pre-exposed to short-term (2 mg O2 L-1), SOD activity in the reoxygenated group pre-exposed to long-term (0.5 mg O2 L-1), and the activities of CAT and GST in all the reoxygenated groups pre-exposed to long-term hypoxia, had no significant change compared to their respective hypoxic groups. The activities of glutathione peroxidase (GPx), glutathione reductase (GR) and xanthine oxidase (XO) significantly increased during hypoxia then decreased significantly after reoxygenation except for GPx activity in the reoxygenated group pre-exposed to short-term (2 mg O2 L-1). Malondialdehyde (MDA) concentrations significantly increased in all the hypoxic and reoxygenated groups compared to their respective controls. The significance of these alterations in enzyme activities and oxidative stress indices is discussed. In conclusion, hypoxia and subsequent reoxygenation induce oxidative stress in Oreochromis niloticus gills causing depletion of some antioxidant enzymes. Long-lasting oxidative stress can delay full recovery.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"17 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134139087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}