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Biochemical changes of the Liver and Kidney of Albino Mice Mus musculus Exposed to Organphosphorus insecticide Imidacloprid (Commando 30%) 有机磷杀虫剂吡虫啉(突击30%)对白化小鼠肝脏、肾脏生化的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190801083542
N. Ibrahim, Elsie Rizk, Ibrahim Shourbagy, Dina Shammari
This study planned to assess the toxicity, biochemical investigation associated with hepatotoxicity and nephrotoxicity of insecticide Imidacloprid (Commando 30%) in addition to serum cholinesterase (chE) activity in albino mice at orally administered sublethal dose of Commando 1/20 of LD50. Results revealed that The LD50 values obtained at 24, 48, 72 and 96 hours exposures and the 95% confidence limits for the imidacloprid (Commando 30%) obtained from the sigmoid curve were 495, 437, 344 and 210 mg/kg bw, respectively. the net body weight gains of the animals intoxicated with sublethal doses of Commando 30% was markedly significant decrease (P≤ 0.5). Significant reduction (P ˂ 0.05) in total protein, and significant increase in ALT, AST, ALP and LDH enzyme activity levels as well as significant decrease in kidney function (urea and creatinine) in all the experimental groups of mice. Serum Cholinestrase (ChE) activity was markedly significant decrease by Commando exposure mice at sublethal dose. The blood parameters of all tested groups, results showed that the median values of blood parameters decreased in animals treated with Commando 30% at a dose of 10.5 mg/kg body weight, indicative of anemic syndrome. In addition, there was a significant reduction in the number of erythrocytes and of hemoglobin concentration, with reduced hematocrit and increased MCV, characteristic of macrocytic anemia.
本研究拟评估杀虫剂吡虫啉(Commando 30%)对白化病小鼠的毒性及与肝毒性和肾毒性相关的生化研究,并对口服Commando 1/20 LD50亚致死剂量的白化病小鼠血清胆碱酯酶(chE)活性进行研究。结果表明,暴露24、48、72和96 h时的LD50值和30%吡虫啉(Commando 30%)的95%置信限分别为495、437、344和210 mg/kg bw。突击队员30%亚致死剂量中毒动物的净增重显著降低(P≤0.5)。各实验组小鼠总蛋白含量均显著降低(P小于0.05),ALT、AST、ALP和LDH酶活性均显著升高,肾功能(尿素和肌酐)均显著降低。亚致死剂量的Commando暴露小鼠血清胆碱酯酶(ChE)活性显著降低。结果显示,给药10.5 mg/kg体重的动物血液参数中位数下降30%,提示贫血综合征。此外,红细胞数量和血红蛋白浓度显著减少,红细胞压积降低,MCV升高,这是大细胞性贫血的特征。
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引用次数: 0
Effect of hypoxia and reoxygenation on antioxidant system in gillsof tilapia fish (Oreochromis niloticus) 缺氧和复氧对罗非鱼(Oreochromis niloticus)氧化系统的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220907064204
Z. Attia, Hala Abdelazem, Iman Nada
The effect of hypoxia and reoxygenation on Nile tilapia (Oreochromis niloticus) was studied. The fish were exposed to hypoxic oxygen concentrations (2 and 0.5 mg O2 L-1) for 1 day (short term) and 30 days (long term) followed by reoxygenation for 1 day and 7 days, respectively. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) significantly decreased in the gills of all the hypoxic groups after both short and long term of hypoxia exposures, while increased significantly below control levels after reoxygenation except that the activities of SOD and GST in the reoxygenated groups pre-exposed to short-term (2 mg O2 L-1), SOD activity in the reoxygenated group pre-exposed to long-term (0.5 mg O2 L-1), and the activities of CAT and GST in all the reoxygenated groups pre-exposed to long-term hypoxia, had no significant change compared to their respective hypoxic groups. The activities of glutathione peroxidase (GPx), glutathione reductase (GR) and xanthine oxidase (XO) significantly increased during hypoxia then decreased significantly after reoxygenation except for GPx activity in the reoxygenated group pre-exposed to short-term (2 mg O2 L-1). Malondialdehyde (MDA) concentrations significantly increased in all the hypoxic and reoxygenated groups compared to their respective controls. The significance of these alterations in enzyme activities and oxidative stress indices is discussed. In conclusion, hypoxia and subsequent reoxygenation induce oxidative stress in Oreochromis niloticus gills causing depletion of some antioxidant enzymes. Long-lasting oxidative stress can delay full recovery.
研究了缺氧和复氧对尼罗罗非鱼(Oreochromis niloticus)的影响。将鱼分别暴露于低氧浓度(2和0.5 mg O2 L-1)中1天(短期)和30天(长期),然后再充氧1天和7天。除短期缺氧预处理组(2 mg O2 L-1)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)活性、长期缺氧预处理组(0.5 mg O2 L-1)超氧化物歧化酶(SOD)活性和长期缺氧预处理组(0.5 mg O2 L-1)超氧化物歧化酶(SOD)活性显著高于对照组外,所有缺氧预处理组的鳃超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)活性均显著低于对照组。各复氧组的CAT和GST活性与各复氧组相比无显著变化。谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)和黄嘌呤氧化酶(XO)活性在缺氧期间显著升高,在复氧后显著降低,除短期(2 mg O2 -1)预氧化组的GPx活性外。与各自的对照组相比,所有缺氧组和再氧组的丙二醛(MDA)浓度显著增加。讨论了酶活性和氧化应激指标变化的意义。综上所述,缺氧和随后的再氧化可引起尼罗鱼鳃的氧化应激,导致一些抗氧化酶的消耗。长期的氧化应激会延迟完全恢复。
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引用次数: 0
Impact of salinity and flaxseed oil -enriched diet on growth and some enzymeactivity of Nile tilapia Oreochromis niloticus 盐度和富亚麻籽油饲料对尼罗罗非鱼生长及部分酶活性的影响
Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190425010119
Menna M. Harby, Elsie Rizk, Ibraheem Shourbagy, M. Bassiouny, M. Wafik
This study was conducted to evaluate the effect of flaxseed oil as a source of omega 3 and salinity factor on growth and some enzyme activity Nile tilapia (Oreochromis niloticus) (mean initial weight 1.18 ± 0.1 g ) in indoor recycle aquarium tanks for 45 days. Results showed that adding flaxseed oil and salinity factors did not alter negatively the growth rate of Nile tilapia. Also, our data showed that fish in salinity group exhibited higher aspartate transaminase and alanine transaminase activities in liver while decreased in the flaxseed oil groups in comparison with the control. In conclusion, rearing Nile tilapia in brackish water can be a viable substitute to fresh water in Nile tilapia aquaculture without affecting the growth of tilapia. Moreover, flaxseed oil could be a viable substitute to the fish oil in Nile tilapia aquaculture without affecting the growth of tilapia. Most importantly, salinity factor can improve some enzyme activities such as ALT and AST in liver of Nile tilapia. .
本试验研究了亚麻籽油作为omega - 3的来源和盐度因子对尼罗罗非鱼(Oreochromis niloticus)生长和部分酶活性的影响。尼罗罗非鱼(Oreochromis niloticus)初始体重为1.18±0.1 g。结果表明,添加亚麻籽油和盐度因素对尼罗罗非鱼的生长速率没有负影响。此外,我们的数据显示,与对照组相比,盐度组鱼的肝脏中天冬氨酸转氨酶和丙氨酸转氨酶活性较高,而亚麻籽油组鱼的肝脏中天冬氨酸转氨酶和丙氨酸转氨酶活性降低。综上所述,在不影响罗非鱼生长的情况下,在微咸水中养殖罗非鱼是一种可行的替代淡水养殖方法。在不影响罗非鱼生长的前提下,亚麻籽油可作为罗非鱼养殖中鱼油的替代饲料。最重要的是,盐度因素可以提高尼罗罗非鱼肝脏中ALT和AST等酶的活性。
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引用次数: 0
Melatonin improves the anticancer effects of mesenchymal stem cell against HCC in rat 褪黑素提高间充质干细胞对大鼠肝癌的抗癌作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190630042451
Mohamed A. Basyony, Nabila Desouki, W. Abdo, R. A. Hegazy, Yasser M. Mohamed
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引用次数: 2
Insecticidal activity of four essential oils against the German, Blattella germanica (L.) and the American, Periplaneta americana (L.) cockroaches 4种精油对德国小蠊和美洲大蠊的杀虫活性研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220211081712
H. Elbrense, M. Aasr
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引用次数: 0
The effect of stevia sweetener on diabetic nephropathy in rats 甜叶菊甜味剂对大鼠糖尿病肾病的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220803073155
A. Marei, Doaa Mahmoud
Diabetic nephropathy (DN) is considered a clinical syndrome, which is a diabetic kidney disease characterized by increasing blood pressure, albuminuria, and chronic kidney disease. The current study aimed to determine the effect of sweetener (Stevia rebaudiana ) on diabetic nephropathy in a diabetic rat model. Four groups (n=7) of male albino rats (Rattus norvegicus): the control group, diabetic control group (induced streptozotocin intraperitoneally), two diabetic rat groups treated with 2 doses of stevia (200 and 400 mg/kg body weight) for 4 weeks. Biomarkers of nephropathy were measured in plasma and kidneys at the end of the experiment. The levels of plasma glucose, urea, uric acid, creatinine, renal malondialdehyde (MDA), and caspase-3 significantly increased in diabetic rats and levels of insulin, renal catalase, superoxide dismutase, and vascular endothelial growth factor A (VEGF-A) were significantly decreased. After treating the diabetic rats with natural sweetener (stevia), the kidney and plasma biochemical parameters were improved significantly. These results indicated the major role of stevia in diabetic nephropathy.
糖尿病肾病(Diabetic nephropathy, DN)被认为是一种临床综合征,是一种以血压升高、蛋白尿和慢性肾脏疾病为特征的糖尿病肾病。目前的研究旨在确定甜味剂(甜菊糖)对糖尿病大鼠模型糖尿病肾病的影响。雄性白化大鼠(褐家鼠)4组(n=7):对照组、糖尿病对照组(腹腔注射链脲佐菌素)、2个糖尿病大鼠组分别给予2剂甜叶菊(200、400 mg/kg体重)治疗4周。在实验结束时,在血浆和肾脏中测量肾病的生物标志物。糖尿病大鼠血浆葡萄糖、尿素、尿酸、肌酐、肾丙二醛(MDA)、caspase-3水平显著升高,胰岛素、肾过氧化氢酶、超氧化物歧化酶、血管内皮生长因子A (VEGF-A)水平显著降低。用天然甜味剂甜叶菊治疗糖尿病大鼠后,肾脏和血浆生化指标均有明显改善。这些结果表明甜叶菊在糖尿病肾病中的主要作用。
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引用次数: 0
Cranberry (Vaccinium macrocarpon) and rosemary (Rosmarinus officinalis) extracts protect against doxorubicin-induced cardiotoxicity in albino rats 蔓越莓(Vaccinium macrocarpon)和迷迭香(Rosmarinus officinalis)提取物对白化大鼠阿霉素诱导的心脏毒性有保护作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190417104454
Nabila Desouki, M. Salem, M. Elwan, Maysa Abosenna
{"title":"Cranberry (Vaccinium macrocarpon) and rosemary (Rosmarinus officinalis) extracts protect against doxorubicin-induced cardiotoxicity in albino rats","authors":"Nabila Desouki, M. Salem, M. Elwan, Maysa Abosenna","doi":"10.5455/egysebz.20190417104454","DOIUrl":"https://doi.org/10.5455/egysebz.20190417104454","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114641729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Protective effect of Origanum majorana against the toxicity of bromadialone on adult male albino rat 牛头草对成年雄性白化大鼠溴二酮毒性的保护作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191201101401
M. Zowail, R. Balshy, A. Asran, Fatma K. Khidr, N. Amer
{"title":"Protective effect of Origanum majorana against the toxicity of bromadialone on adult male albino rat","authors":"M. Zowail, R. Balshy, A. Asran, Fatma K. Khidr, N. Amer","doi":"10.5455/egysebz.20191201101401","DOIUrl":"https://doi.org/10.5455/egysebz.20191201101401","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123720693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Histological, ultrastructural, and biochemical studies on the expected role of thyme oil on ameliorating renal toxicity induced by doxorubicin in female albino rats 百里香油改善阿霉素所致雌性白化大鼠肾毒性的组织学、超微结构和生化研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220507065051
E. Kandil, Y. Okdah, Ayat Moselhy
Doxorubicin is antibiotics anticancer drug which proved to be useful in treatment of cancer, but it has bad effect on healthy body organs. Thymus vulgaris (thyme) cultivated around the world for cosmetic, cooking, and medical uses. So, the present work aims to investigate the possible ameliorating effect of thyme oil on renal toxicity induced by doxorubicin. Adult female albino rats (Rattus norvegicus) were randomly divided into four groups (5 rats/group): control group, thyme group received orally 0.5 mL thyme oil/kg body weight once/week for 6 consecutive weeks, doxorubicin group received intraperitoneally 2 mg doxorubicin/kg body weight once/week for 6 consecutive weeks, and doxorubicin + thyme group received both doxorubicin and thyme oil once/week for 6 consecutive weeks. A significant rising in urea and creatinine level in the sera of rats injected with doxorubicin was recorded. Moreover, their kidney sections showed degenerated, vacuolated and little hemorrhage glomeruli, abnormal renal tubular cells, aggregation of mononuclear leucocytes and hyaline cast. Ultrastructure examination showed degenerated glomerular endothelial, untypical filtrating barrier, degenerated podocyte, mesangial cell, pathological proximal and distal tubular cell with damage microvilli. Treating rats with thyme oil after doxorubicin decreases the elevated levels of urea and creatinine. The glomeruli, Podocyte, intraglomerular mesangial cells and glomerular filtration barrier approximately restored its typical structure, the winding in Bouma’s space is disappeared. Most tubular cells have normal nuclei and cytoplasm. Thyme oil can improve renal functions, histological and ultrastructural changes induced by doxorubicin. So, it can be used to decrease its side effect on kidney.Keywords: Doxorubicin, Histology, Ultrastructure, Thyme oil, urea, creatinine.
阿霉素是一种抗菌素抗癌药,在治疗癌症方面有较好的效果,但对健康的身体器官有不良影响。世界各地种植的百里香,用于化妆品、烹饪和医疗用途。因此,本研究旨在探讨百里香油对阿霉素所致肾毒性的可能改善作用。将成年雌性褐家鼠(Rattus norvegicus)随机分为4组(5只/组):对照组、百里香组口服百里香油0.5 mL /kg体重1次/周,连续6周;阿霉素组腹腔注射阿霉素2 mg /kg体重1次/周,连续6周;阿霉素+百里香组同时服用阿霉素和百里香油1次/周,连续6周。大鼠注射阿霉素后血清尿素和肌酐水平明显升高。肾切片示肾小球变性、空泡化、少量出血,肾小管细胞异常,单核白细胞聚集,铸型透明。超微结构检查显示肾小球内皮变性,滤过屏障不典型,足细胞、系膜细胞变性,近端和远端小管细胞病理性,微绒毛受损。用百里香油治疗阿霉素后降低了尿素和肌酐的升高水平。肾小球、足细胞、肾小球系膜细胞及肾小球滤过屏障大致恢复其典型结构,鲍氏间隙内的缠绕消失。大多数小管细胞有正常的细胞核和细胞质。百里香油能改善阿霉素所致肾功能及组织超微结构的改变。因此,它可以用来减少其对肾脏的副作用。关键词:阿霉素,组织学,超微结构,百里香油,尿素,肌酐。
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引用次数: 0
Effect of nutritional quality of rotifers and copepods on sea bream (Sparus aurata) fry fish productivity 轮虫和桡足类营养质量对真鲷鱼苗产量的影响
Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190303091611
M. Mona, El-Sayed T. Rizk, M. Fiky, Mona Elawany
The present study aims to evaluate the effects of zooplankton rotifers and copepods on sea bream Sparus aurata fry fish productivity concerning their growth performance, production, and feeding utilization. Two hundred seventy fry of the sea bream (Sparus aurata) with a mean body weight of 0.11±0.01 g were collected from fish hatchery, Alexandria, Egypt and transported to the fish rearing unit in El-Max Research Station, National Institute of Oceanography and Fishers (NIOF), Alexandria, Egypt and maintained under standard laboratory conditions. Fry fish were divided into 3 experimental groups, 120 fish each. The feeding regime of experimental group was: group 1 (T1) fed natural rotifer diet with 20% commercial diet, group 2 (T2) fed natural copepod diet with 20% commercial diet, group 3 (T3) fed natural rotifer and copepod with 20% commercial diet. The feeding was provided four times a day (8.00 &10:00 am and 12.00 & 2:00pm) at a rate of 15% of the live body weight of the fish. The present results revealed that the environmental factors: temperatures (20°C, 25°C, 30°C) and salinities (20 ppt, 25 ppt, 30 ppt) significantly affect the total number and the growth performance of rotifers (Brachionus plicatilis) and copepods (Cyclops vernalis). Additionally mixing feeding (rotifers with copepods) resulted in significant increase the carbohydrates and lipids percentage in fry fish comparing to feeding with diet
本研究旨在评估浮游动物轮虫和桡足类对真鲷鱼苗生长性能、产量和摄食利用率的影响。从埃及亚历山大鱼苗孵化场收集了 270 尾平均体重为 0.11±0.01 g 的鲷鱼苗,将其运到埃及亚历山大国家海洋学和渔业研究所(NIOF)El-Max 研究站的鱼类饲养单元,并在标准实验室条件下进行饲养。鱼苗被分为 3 个实验组,每组 120 尾。实验组的投喂方式为:第 1 组(T1)投喂天然轮虫饲料和 20% 的商品饲料;第 2 组(T2)投喂天然桡足类饲料和 20% 的商品饲料;第 3 组(T3)投喂天然轮虫和桡足类饲料和 20% 的商品饲料。每天投喂四次(上午 8:00 和 10:00,中午 12:00 和下午 2:00),投喂量为活鱼体重的 15%。本研究结果表明,环境因素:温度(20°C、25°C、30°C)和盐度(20 ppt、25 ppt、30 ppt)会显著影响轮虫(Brachionus plicatilis)和桡足类(Cyclops vernalis)的总数和生长表现。此外,轮虫与桡足类混合投喂与投喂日粮相比,鱼苗体内碳水化合物和脂类的比例明显增加。
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引用次数: 7
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THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)
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