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Effect of hypoxia and reoxygenation on antioxidant system in gillsof tilapia fish (Oreochromis niloticus) 缺氧和复氧对罗非鱼(Oreochromis niloticus)氧化系统的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220907064204
Z. Attia, Hala Abdelazem, Iman Nada
The effect of hypoxia and reoxygenation on Nile tilapia (Oreochromis niloticus) was studied. The fish were exposed to hypoxic oxygen concentrations (2 and 0.5 mg O2 L-1) for 1 day (short term) and 30 days (long term) followed by reoxygenation for 1 day and 7 days, respectively. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) significantly decreased in the gills of all the hypoxic groups after both short and long term of hypoxia exposures, while increased significantly below control levels after reoxygenation except that the activities of SOD and GST in the reoxygenated groups pre-exposed to short-term (2 mg O2 L-1), SOD activity in the reoxygenated group pre-exposed to long-term (0.5 mg O2 L-1), and the activities of CAT and GST in all the reoxygenated groups pre-exposed to long-term hypoxia, had no significant change compared to their respective hypoxic groups. The activities of glutathione peroxidase (GPx), glutathione reductase (GR) and xanthine oxidase (XO) significantly increased during hypoxia then decreased significantly after reoxygenation except for GPx activity in the reoxygenated group pre-exposed to short-term (2 mg O2 L-1). Malondialdehyde (MDA) concentrations significantly increased in all the hypoxic and reoxygenated groups compared to their respective controls. The significance of these alterations in enzyme activities and oxidative stress indices is discussed. In conclusion, hypoxia and subsequent reoxygenation induce oxidative stress in Oreochromis niloticus gills causing depletion of some antioxidant enzymes. Long-lasting oxidative stress can delay full recovery.
研究了缺氧和复氧对尼罗罗非鱼(Oreochromis niloticus)的影响。将鱼分别暴露于低氧浓度(2和0.5 mg O2 L-1)中1天(短期)和30天(长期),然后再充氧1天和7天。除短期缺氧预处理组(2 mg O2 L-1)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)活性、长期缺氧预处理组(0.5 mg O2 L-1)超氧化物歧化酶(SOD)活性和长期缺氧预处理组(0.5 mg O2 L-1)超氧化物歧化酶(SOD)活性显著高于对照组外,所有缺氧预处理组的鳃超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)活性均显著低于对照组。各复氧组的CAT和GST活性与各复氧组相比无显著变化。谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)和黄嘌呤氧化酶(XO)活性在缺氧期间显著升高,在复氧后显著降低,除短期(2 mg O2 -1)预氧化组的GPx活性外。与各自的对照组相比,所有缺氧组和再氧组的丙二醛(MDA)浓度显著增加。讨论了酶活性和氧化应激指标变化的意义。综上所述,缺氧和随后的再氧化可引起尼罗鱼鳃的氧化应激,导致一些抗氧化酶的消耗。长期的氧化应激会延迟完全恢复。
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引用次数: 0
Subtropical benthic freshwater nematode response to Low versus High water level alteration 亚热带底栖淡水线虫对高低水位变化的响应
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20230201023633
Ahmed Abada
Quantitative and qualitative responses of subtropical benthic freshwater nematodes to changing water levels (low versus high) have not been considered before. Three distinct locations with different depths in two freshwater channels (Rowaina and Shalma) of the northern Nile delta had been chosen for this purpose. Low and High-water levels (LWL & HWL) were in January and July 2019 respectively. Nematode abundance and diversity were significantly different between water levels, locations, and their interactions. They were categorized into six feeding groups in both water levels, locations and channels (bacterivores, plant ectoparasites, fungivores, Nematovorous, omnivorous, and predators). However, bacterivorous and omnivorous disappeared during HWL in Rowaina channel. The relative dominance of the feeding groups differed between water levels, localities, and channels. Dominancy was generally higher in Shalma. Diversity pattern in Rowaina contrasted that of Shalma. Environmental variables differently influenced nematode community. It could be concluded that water level fluctuation was not the only factor determining benthic nematode abundance, diversity, and trophic categorization. Specific water body conditions affect benthic nematode community structure. Nematode communities are environmentally sensitive agent. Furthermore, the current findings might improve our understanding of aquaculture, as water level fluctuations affect benthic nematodes, which directly or indirectly affect the reared organisms.
亚热带底栖淡水线虫对水位(高低)变化的定量和定性反应以前从未被考虑过。为此,在尼罗河三角洲北部的两条淡水水道(罗瓦伊纳和沙尔马)中选择了三个不同深度的不同地点。低水位和高水位(LWL和HWL)分别在2019年1月和7月。线虫的丰度和多样性在不同的水位、地点及其相互作用之间存在显著差异。它们在水位、位置和渠道上被分为6个摄食组(食细菌者、植物外寄生虫、食真菌者、线虫性、杂食性和捕食者)。然而,在罗维纳海峡的HWL期间,细菌食性和杂食性消失了。在不同的水位、不同的地区和不同的河道中,进食群体的相对优势是不同的。沙尔马的优势度普遍较高。罗维纳岛的多样性格局与沙尔马岛形成对比。环境变量对线虫群落的影响不同。由此可见,水位波动不是决定底栖线虫丰度、多样性和营养分类的唯一因素。特定的水体条件影响底栖线虫群落结构。线虫群落对环境敏感。此外,目前的研究结果可能会提高我们对水产养殖的理解,因为水位波动会影响底栖线虫,从而直接或间接地影响养殖的生物。
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引用次数: 0
Impact of salinity and flaxseed oil -enriched diet on growth and some enzymeactivity of Nile tilapia Oreochromis niloticus 盐度和富亚麻籽油饲料对尼罗罗非鱼生长及部分酶活性的影响
Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190425010119
Menna M. Harby, Elsie Rizk, Ibraheem Shourbagy, M. Bassiouny, M. Wafik
This study was conducted to evaluate the effect of flaxseed oil as a source of omega 3 and salinity factor on growth and some enzyme activity Nile tilapia (Oreochromis niloticus) (mean initial weight 1.18 ± 0.1 g ) in indoor recycle aquarium tanks for 45 days. Results showed that adding flaxseed oil and salinity factors did not alter negatively the growth rate of Nile tilapia. Also, our data showed that fish in salinity group exhibited higher aspartate transaminase and alanine transaminase activities in liver while decreased in the flaxseed oil groups in comparison with the control. In conclusion, rearing Nile tilapia in brackish water can be a viable substitute to fresh water in Nile tilapia aquaculture without affecting the growth of tilapia. Moreover, flaxseed oil could be a viable substitute to the fish oil in Nile tilapia aquaculture without affecting the growth of tilapia. Most importantly, salinity factor can improve some enzyme activities such as ALT and AST in liver of Nile tilapia. .
本试验研究了亚麻籽油作为omega - 3的来源和盐度因子对尼罗罗非鱼(Oreochromis niloticus)生长和部分酶活性的影响。尼罗罗非鱼(Oreochromis niloticus)初始体重为1.18±0.1 g。结果表明,添加亚麻籽油和盐度因素对尼罗罗非鱼的生长速率没有负影响。此外,我们的数据显示,与对照组相比,盐度组鱼的肝脏中天冬氨酸转氨酶和丙氨酸转氨酶活性较高,而亚麻籽油组鱼的肝脏中天冬氨酸转氨酶和丙氨酸转氨酶活性降低。综上所述,在不影响罗非鱼生长的情况下,在微咸水中养殖罗非鱼是一种可行的替代淡水养殖方法。在不影响罗非鱼生长的前提下,亚麻籽油可作为罗非鱼养殖中鱼油的替代饲料。最重要的是,盐度因素可以提高尼罗罗非鱼肝脏中ALT和AST等酶的活性。
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引用次数: 0
Insecticidal activity of four essential oils against the German, Blattella germanica (L.) and the American, Periplaneta americana (L.) cockroaches 4种精油对德国小蠊和美洲大蠊的杀虫活性研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220211081712
H. Elbrense, M. Aasr
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引用次数: 0
The effect of stevia sweetener on diabetic nephropathy in rats 甜叶菊甜味剂对大鼠糖尿病肾病的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220803073155
A. Marei, Doaa Mahmoud
Diabetic nephropathy (DN) is considered a clinical syndrome, which is a diabetic kidney disease characterized by increasing blood pressure, albuminuria, and chronic kidney disease. The current study aimed to determine the effect of sweetener (Stevia rebaudiana ) on diabetic nephropathy in a diabetic rat model. Four groups (n=7) of male albino rats (Rattus norvegicus): the control group, diabetic control group (induced streptozotocin intraperitoneally), two diabetic rat groups treated with 2 doses of stevia (200 and 400 mg/kg body weight) for 4 weeks. Biomarkers of nephropathy were measured in plasma and kidneys at the end of the experiment. The levels of plasma glucose, urea, uric acid, creatinine, renal malondialdehyde (MDA), and caspase-3 significantly increased in diabetic rats and levels of insulin, renal catalase, superoxide dismutase, and vascular endothelial growth factor A (VEGF-A) were significantly decreased. After treating the diabetic rats with natural sweetener (stevia), the kidney and plasma biochemical parameters were improved significantly. These results indicated the major role of stevia in diabetic nephropathy.
糖尿病肾病(Diabetic nephropathy, DN)被认为是一种临床综合征,是一种以血压升高、蛋白尿和慢性肾脏疾病为特征的糖尿病肾病。目前的研究旨在确定甜味剂(甜菊糖)对糖尿病大鼠模型糖尿病肾病的影响。雄性白化大鼠(褐家鼠)4组(n=7):对照组、糖尿病对照组(腹腔注射链脲佐菌素)、2个糖尿病大鼠组分别给予2剂甜叶菊(200、400 mg/kg体重)治疗4周。在实验结束时,在血浆和肾脏中测量肾病的生物标志物。糖尿病大鼠血浆葡萄糖、尿素、尿酸、肌酐、肾丙二醛(MDA)、caspase-3水平显著升高,胰岛素、肾过氧化氢酶、超氧化物歧化酶、血管内皮生长因子A (VEGF-A)水平显著降低。用天然甜味剂甜叶菊治疗糖尿病大鼠后,肾脏和血浆生化指标均有明显改善。这些结果表明甜叶菊在糖尿病肾病中的主要作用。
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引用次数: 0
Melatonin improves the anticancer effects of mesenchymal stem cell against HCC in rat 褪黑素提高间充质干细胞对大鼠肝癌的抗癌作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190630042451
Mohamed A. Basyony, Nabila Desouki, W. Abdo, R. A. Hegazy, Yasser M. Mohamed
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引用次数: 2
Neuroprotective Effect of Chrysin in Rat Model of Parkinsons Disease: Histopathological Evidence 黄菊花素在帕金森病大鼠模型中的神经保护作用:组织病理学证据
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20210723020047
A. Gaballa, H. Swelim, A. Aal, S. Eldawlatly
{"title":"Neuroprotective Effect of Chrysin in Rat Model of Parkinsons Disease: Histopathological Evidence","authors":"A. Gaballa, H. Swelim, A. Aal, S. Eldawlatly","doi":"10.5455/egysebz.20210723020047","DOIUrl":"https://doi.org/10.5455/egysebz.20210723020047","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"164 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116395247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological, ultrastructural, and biochemical studies on the expected role of thyme oil on ameliorating renal toxicity induced by doxorubicin in female albino rats 百里香油改善阿霉素所致雌性白化大鼠肾毒性的组织学、超微结构和生化研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220507065051
E. Kandil, Y. Okdah, Ayat Moselhy
Doxorubicin is antibiotics anticancer drug which proved to be useful in treatment of cancer, but it has bad effect on healthy body organs. Thymus vulgaris (thyme) cultivated around the world for cosmetic, cooking, and medical uses. So, the present work aims to investigate the possible ameliorating effect of thyme oil on renal toxicity induced by doxorubicin. Adult female albino rats (Rattus norvegicus) were randomly divided into four groups (5 rats/group): control group, thyme group received orally 0.5 mL thyme oil/kg body weight once/week for 6 consecutive weeks, doxorubicin group received intraperitoneally 2 mg doxorubicin/kg body weight once/week for 6 consecutive weeks, and doxorubicin + thyme group received both doxorubicin and thyme oil once/week for 6 consecutive weeks. A significant rising in urea and creatinine level in the sera of rats injected with doxorubicin was recorded. Moreover, their kidney sections showed degenerated, vacuolated and little hemorrhage glomeruli, abnormal renal tubular cells, aggregation of mononuclear leucocytes and hyaline cast. Ultrastructure examination showed degenerated glomerular endothelial, untypical filtrating barrier, degenerated podocyte, mesangial cell, pathological proximal and distal tubular cell with damage microvilli. Treating rats with thyme oil after doxorubicin decreases the elevated levels of urea and creatinine. The glomeruli, Podocyte, intraglomerular mesangial cells and glomerular filtration barrier approximately restored its typical structure, the winding in Bouma’s space is disappeared. Most tubular cells have normal nuclei and cytoplasm. Thyme oil can improve renal functions, histological and ultrastructural changes induced by doxorubicin. So, it can be used to decrease its side effect on kidney.Keywords: Doxorubicin, Histology, Ultrastructure, Thyme oil, urea, creatinine.
阿霉素是一种抗菌素抗癌药,在治疗癌症方面有较好的效果,但对健康的身体器官有不良影响。世界各地种植的百里香,用于化妆品、烹饪和医疗用途。因此,本研究旨在探讨百里香油对阿霉素所致肾毒性的可能改善作用。将成年雌性褐家鼠(Rattus norvegicus)随机分为4组(5只/组):对照组、百里香组口服百里香油0.5 mL /kg体重1次/周,连续6周;阿霉素组腹腔注射阿霉素2 mg /kg体重1次/周,连续6周;阿霉素+百里香组同时服用阿霉素和百里香油1次/周,连续6周。大鼠注射阿霉素后血清尿素和肌酐水平明显升高。肾切片示肾小球变性、空泡化、少量出血,肾小管细胞异常,单核白细胞聚集,铸型透明。超微结构检查显示肾小球内皮变性,滤过屏障不典型,足细胞、系膜细胞变性,近端和远端小管细胞病理性,微绒毛受损。用百里香油治疗阿霉素后降低了尿素和肌酐的升高水平。肾小球、足细胞、肾小球系膜细胞及肾小球滤过屏障大致恢复其典型结构,鲍氏间隙内的缠绕消失。大多数小管细胞有正常的细胞核和细胞质。百里香油能改善阿霉素所致肾功能及组织超微结构的改变。因此,它可以用来减少其对肾脏的副作用。关键词:阿霉素,组织学,超微结构,百里香油,尿素,肌酐。
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引用次数: 0
Histopathological alterations of submandibular salivary glands in Experimental Animal Albino Rat Induced by excess vitamin A 过量维生素A诱导实验动物白化大鼠下颌下唾液腺的组织病理学改变
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190723094132
and kasheif
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引用次数: 0
Comparative taxonomical studies on the Egyptian Mediterranean Squids 埃及地中海鱿鱼的比较分类学研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20210608120218
R. Riad
Squids are known to be commercially important around the world. Worth mentioning it constitutes a major part of the Egyptian fishing industry. This study aims to differentiate between the teuthoid species dwelling in the Egyptian Mediterranean waters. Squid Specimens were obtained from the fishing trawlers. The specimens were photographed by a CanonG7X digital Camera and the different organs were illustrated using a zoom stereoscopic WildM8 microscope provided with its special Camera Lucida drawing tube.
鱿鱼在世界各地都有重要的商业价值。值得一提的是,它是埃及渔业的重要组成部分。本研究的目的是区分居住在埃及地中海水域的双齿鱼种类。从拖网渔船上获得鱿鱼标本。用CanonG7X数码相机对标本进行拍摄,用带有专用Camera Lucida画管的变焦立体WildM8显微镜对不同器官进行图示。
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引用次数: 0
期刊
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)
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