Pub Date : 1900-01-01DOI: 10.5455/egysebz.20210701062534
Fouad Zaid, E. Salim, S. Naggar, M. Maghawry
{"title":"Physiological and histological alterations after post chronic treatment with calcium disodium EDTA on experimental male rats","authors":"Fouad Zaid, E. Salim, S. Naggar, M. Maghawry","doi":"10.5455/egysebz.20210701062534","DOIUrl":"https://doi.org/10.5455/egysebz.20210701062534","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"369 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125298624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/EGYSEBZ.20190712010741
R. Mansour, Hewaydah E. Abou Shafeey
Abstract The developmental stages of a new Isospora species, I. vittati, were described from the bridled skink Trachylepis vittata for the first time in Egypt. Fifty four skinks were collected in April and May 2019 from Baltim, Kafr El Sheikh Governorate, Egypt and examined for the presence of Coccidia. The prevalence recorded as 24.1% and it was higher in males than females. Oocysts were shed unsporulated, in early stages of sporulation and rarely sporulated. Events of sporulation and sporulation time were described. The sporulated oocysts, limited by bilayered wall, were spherical to subspherical measuring 21-25.6 x 19.2-23.4 μm. No micropyle, polar granule or oocyst residuum was noticed. The sporocysts were ovoid to ellipsoidal measuring 13.2-15 x 8.5-9.5 μm. Stieda, substieda bodies and sporocyst residuum were present. Experimental infection by sporulated oocysts was carried out and the developmental endogenous stages (merogony and gamogony) were followed up and described. The prepatent and patent periods were 6 and 4 days, respectively. Endogenous stages took place in the lamina propria and enterocytes of small intestine of the experimentally infected skinks. One generation merogony was occurred at 24-72 h p.i. The mature meronts measured 5.5- 18.8 x 5.4-17.3 μm and yielded up to 28 merozoites. Gamogony occurred at 84-96 h p.i. The mature microgamonts measured 11.1-12.6 x 8.4-11.2 μm and contained 16-40 small nuclei while the mature macrogamonts measured 15.2-18.2 x 14.8-16.4 μm and contained a large central nucleus and the two types of wall-forming bodies. Newly-formed zygotes or young oocysts were seen at 120 h p.i. measuring 16.8-20.2 x 14.2-17.8 μm. In addition, the checklist of Isospora spp. infecting the Egyptian reptiles, including the present species, was provided.
摘要在埃及首次从有笼头的石龙子(Trachylepis vittata)身上描述了一种新的等孢子目物种I. vittati的发育阶段。2019年4月和5月,从埃及Kafr El Sheikh省的Baltim收集了54只石龙子,并检查了球虫的存在。患病率为24.1%,男性高于女性。卵囊脱落无孢子,在孢子形成的早期,很少有孢子。描述了产孢事件和产孢时间。孢子卵囊受双层壁限制,尺寸为21-25.6 x 19.2-23.4 μm,呈球形至亚球形。未见微孔、极性颗粒或卵囊残留。孢子囊大小为13.2 ~ 15 × 8.5 ~ 9.5 μm,呈卵形至椭球形。存在孢子体、代孢子体和孢子囊残体。实验采用孢子卵囊感染,并对其内源发育阶段(单性生殖和配子生殖)进行了跟踪和描述。专利前和专利期分别为6天和4天。内源性阶段发生在实验感染石龙子的固有层和小肠细胞。在24 ~ 72h发生了1代分生,成熟的分生子尺寸为5.5 ~ 18.8 x 5.4 ~ 17.3 μm,产生了多达28个分生子。成熟的小胞体尺寸为11.1 ~ 12.6 x 8.4 ~ 11.2 μm,含有16 ~ 40个小核;成熟的大胞体尺寸为15.2 ~ 18.2 x 14.8 ~ 16.4 μm,含有一个大的中心核和两种类型的成壁体。120h p.i.见新形成的受精卵或幼卵囊,尺寸为16.8 ~ 20.2 x 14.2 ~ 17.8 μm。此外,还提供了感染埃及爬行动物(包括本种)的异孢子虫的清单。
{"title":"Life cycle of Isospora vittati n. sp. (Apicomplexa: Eimeriidae) infecting the bridled skink Trachylepis (Syn. Mabuya) vittata (Reptilia: Scincidae) in Egypt with checklist of Isospora spp. infecting the Egyptian reptilian fauna","authors":"R. Mansour, Hewaydah E. Abou Shafeey","doi":"10.5455/EGYSEBZ.20190712010741","DOIUrl":"https://doi.org/10.5455/EGYSEBZ.20190712010741","url":null,"abstract":"Abstract \u0000The developmental stages of a new Isospora species, I. vittati, were described from the bridled skink Trachylepis vittata for the first time in Egypt. Fifty four skinks were collected in April and May 2019 from Baltim, Kafr El Sheikh Governorate, Egypt and examined for the presence of Coccidia. The prevalence recorded as 24.1% and it was higher in males than females. Oocysts were shed unsporulated, in early stages of sporulation and rarely sporulated. Events of sporulation and sporulation time were described. The sporulated oocysts, limited by bilayered wall, were spherical to subspherical measuring 21-25.6 x 19.2-23.4 μm. No micropyle, polar granule or oocyst residuum was noticed. The sporocysts were ovoid to ellipsoidal measuring 13.2-15 x 8.5-9.5 μm. Stieda, substieda bodies and sporocyst residuum were present. Experimental infection by sporulated oocysts was carried out and the developmental endogenous stages (merogony and gamogony) were followed up and described. The prepatent and patent periods were 6 and 4 days, respectively. Endogenous stages took place in the lamina propria and enterocytes of small intestine of the experimentally infected skinks. One generation merogony was occurred at 24-72 h p.i. The mature meronts measured 5.5- 18.8 x 5.4-17.3 μm and yielded up to 28 merozoites. Gamogony occurred at 84-96 h p.i. The mature microgamonts measured 11.1-12.6 x 8.4-11.2 μm and contained 16-40 small nuclei while the mature macrogamonts measured 15.2-18.2 x 14.8-16.4 μm and contained a large central nucleus and the two types of wall-forming bodies. Newly-formed zygotes or young oocysts were seen at 120 h p.i. measuring 16.8-20.2 x 14.2-17.8 μm. In addition, the checklist of Isospora spp. infecting the Egyptian reptiles, including the present species, was provided.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122737077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/egysebz.20191129034810
M. Zowail, R. Balshy, A. Asran, Fatma K. Mohammed, Ghada G. Zaki
Brodifacoum is a 4-hydroxycoumarin anticoagulant rodenticide of Rodents. This denotes a high possibility that contaminated the diet of man and his farm animals this fact alarmed some scientists to study their biological influence. Ginger (Zingiber officinale) has been used as an antioxidant and preventing agent against numbers of diseases. The study deals with effects of brodifacoum on the chromosome and on the liver and testis of male mice as well as and the protective effects of ginger extract. 130 Adult albino male mice were used in this study. Group (G1) served as control. Groups (G2&3) was received ginger extract doses 1.5% and 3%. Groups (G4&5) received single dose (1/10 LD50) of brodifacoum for group (G4) and (1/20 LD50) for group (G5). Groups (G6, 7, 8 & 9) received brodifacoum then ginger extract. Groups (G10, 11, 12&13) received ginger extract then brodifacoum. Animals from all the groups were sacrificed and their bone marrow cells were analyzed for chromosomal damage. Liver and testis samples are collected for histopathological examination. The animals of the groups that treated with brodifacoum showed significant increase of chromosomal aberrations and the value of mitotic index were reduced. Treated male mice with brodifacoum induced various histological changes in liver and testis. Treated animals with brodifacoum and ginger lead to reduction in chromosomal aberrations, significant increase in mitotic index and slight improvement in the histological change in liver and testis. Results obtained from this study demonstrated that brodifacoum has a mutagenic effect on chromosome and histopathological change in different organ (liver and testis) and the use of ginger may be a potent protective against the toxicity of brodifacoum anticoagulant rodenticide.
{"title":"Effect of GINGER AGAINST THE TOXICITY OF BRODIFACOUM \u0000ON ALBINO MALE MICE","authors":"M. Zowail, R. Balshy, A. Asran, Fatma K. Mohammed, Ghada G. Zaki","doi":"10.5455/egysebz.20191129034810","DOIUrl":"https://doi.org/10.5455/egysebz.20191129034810","url":null,"abstract":"Brodifacoum is a 4-hydroxycoumarin anticoagulant rodenticide of Rodents. This denotes a high possibility that contaminated the diet of man and his farm animals this fact alarmed some scientists to study their biological influence. Ginger (Zingiber officinale) has been used as an antioxidant and preventing agent against numbers of diseases. The study deals with effects of brodifacoum on the chromosome and on the liver and testis of male mice as well as and the protective effects of ginger extract. 130 Adult albino male mice were used in this study. Group (G1) served as control. Groups (G2&3) was received ginger extract doses 1.5% and 3%. Groups (G4&5) received single dose (1/10 LD50) of brodifacoum for group (G4) and (1/20 LD50) for group (G5). Groups (G6, 7, 8 & 9) received brodifacoum then ginger extract. Groups (G10, 11, 12&13) received ginger extract then brodifacoum. Animals from all the groups were sacrificed and their bone marrow cells were analyzed for chromosomal damage. Liver and testis samples are collected for histopathological examination. The animals of the groups that treated with brodifacoum showed significant increase of chromosomal aberrations and the value of mitotic index were reduced. Treated male mice with brodifacoum induced various histological changes in liver and testis. Treated animals with brodifacoum and ginger lead to reduction in chromosomal aberrations, significant increase in mitotic index and slight improvement in the histological change in liver and testis. Results obtained from this study demonstrated that brodifacoum has a mutagenic effect on chromosome and histopathological change in different organ (liver and testis) and the use of ginger may be a potent protective against the toxicity of brodifacoum anticoagulant rodenticide.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125793864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}