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Physiological and histological alterations after post chronic treatment with calcium disodium EDTA on experimental male rats EDTA二钠钙对实验性雄性大鼠慢性治疗后的生理和组织学改变
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20210701062534
Fouad Zaid, E. Salim, S. Naggar, M. Maghawry
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引用次数: 0
Life cycle of Isospora vittati n. sp. (Apicomplexa: Eimeriidae) infecting the bridled skink Trachylepis (Syn. Mabuya) vittata (Reptilia: Scincidae) in Egypt with checklist of Isospora spp. infecting the Egyptian reptilian fauna 埃及爬行动物(爬行动物目:爬行动物科)感染的维塔异孢子虫(顶复体目:艾拉螨科)的生命周期与感染埃及爬行动物的维塔异孢子虫清单
Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190712010741
R. Mansour, Hewaydah E. Abou Shafeey
Abstract The developmental stages of a new Isospora species, I. vittati, were described from the bridled skink Trachylepis vittata for the first time in Egypt. Fifty four skinks were collected in April and May 2019 from Baltim, Kafr El Sheikh Governorate, Egypt and examined for the presence of Coccidia. The prevalence recorded as 24.1% and it was higher in males than females. Oocysts were shed unsporulated, in early stages of sporulation and rarely sporulated. Events of sporulation and sporulation time were described. The sporulated oocysts, limited by bilayered wall, were spherical to subspherical measuring 21-25.6 x 19.2-23.4 μm. No micropyle, polar granule or oocyst residuum was noticed. The sporocysts were ovoid to ellipsoidal measuring 13.2-15 x 8.5-9.5 μm. Stieda, substieda bodies and sporocyst residuum were present. Experimental infection by sporulated oocysts was carried out and the developmental endogenous stages (merogony and gamogony) were followed up and described. The prepatent and patent periods were 6 and 4 days, respectively. Endogenous stages took place in the lamina propria and enterocytes of small intestine of the experimentally infected skinks. One generation merogony was occurred at 24-72 h p.i. The mature meronts measured 5.5- 18.8 x 5.4-17.3 μm and yielded up to 28 merozoites. Gamogony occurred at 84-96 h p.i. The mature microgamonts measured 11.1-12.6 x 8.4-11.2 μm and contained 16-40 small nuclei while the mature macrogamonts measured 15.2-18.2 x 14.8-16.4 μm and contained a large central nucleus and the two types of wall-forming bodies. Newly-formed zygotes or young oocysts were seen at 120 h p.i. measuring 16.8-20.2 x 14.2-17.8 μm. In addition, the checklist of Isospora spp. infecting the Egyptian reptiles, including the present species, was provided.
摘要在埃及首次从有笼头的石龙子(Trachylepis vittata)身上描述了一种新的等孢子目物种I. vittati的发育阶段。2019年4月和5月,从埃及Kafr El Sheikh省的Baltim收集了54只石龙子,并检查了球虫的存在。患病率为24.1%,男性高于女性。卵囊脱落无孢子,在孢子形成的早期,很少有孢子。描述了产孢事件和产孢时间。孢子卵囊受双层壁限制,尺寸为21-25.6 x 19.2-23.4 μm,呈球形至亚球形。未见微孔、极性颗粒或卵囊残留。孢子囊大小为13.2 ~ 15 × 8.5 ~ 9.5 μm,呈卵形至椭球形。存在孢子体、代孢子体和孢子囊残体。实验采用孢子卵囊感染,并对其内源发育阶段(单性生殖和配子生殖)进行了跟踪和描述。专利前和专利期分别为6天和4天。内源性阶段发生在实验感染石龙子的固有层和小肠细胞。在24 ~ 72h发生了1代分生,成熟的分生子尺寸为5.5 ~ 18.8 x 5.4 ~ 17.3 μm,产生了多达28个分生子。成熟的小胞体尺寸为11.1 ~ 12.6 x 8.4 ~ 11.2 μm,含有16 ~ 40个小核;成熟的大胞体尺寸为15.2 ~ 18.2 x 14.8 ~ 16.4 μm,含有一个大的中心核和两种类型的成壁体。120h p.i.见新形成的受精卵或幼卵囊,尺寸为16.8 ~ 20.2 x 14.2 ~ 17.8 μm。此外,还提供了感染埃及爬行动物(包括本种)的异孢子虫的清单。
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引用次数: 1
Effect of GINGER AGAINST THE TOXICITY OF BRODIFACOUM ON ALBINO MALE MICE 姜抗溴法菌对白化雄性小鼠的毒性作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191129034810
M. Zowail, R. Balshy, A. Asran, Fatma K. Mohammed, Ghada G. Zaki
Brodifacoum is a 4-hydroxycoumarin anticoagulant rodenticide of Rodents. This denotes a high possibility that contaminated the diet of man and his farm animals this fact alarmed some scientists to study their biological influence. Ginger (Zingiber officinale) has been used as an antioxidant and preventing agent against numbers of diseases. The study deals with effects of brodifacoum on the chromosome and on the liver and testis of male mice as well as and the protective effects of ginger extract. 130 Adult albino male mice were used in this study. Group (G1) served as control. Groups (G2&3) was received ginger extract doses 1.5% and 3%. Groups (G4&5) received single dose (1/10 LD50) of brodifacoum for group (G4) and (1/20 LD50) for group (G5). Groups (G6, 7, 8 & 9) received brodifacoum then ginger extract. Groups (G10, 11, 12&13) received ginger extract then brodifacoum. Animals from all the groups were sacrificed and their bone marrow cells were analyzed for chromosomal damage. Liver and testis samples are collected for histopathological examination. The animals of the groups that treated with brodifacoum showed significant increase of chromosomal aberrations and the value of mitotic index were reduced. Treated male mice with brodifacoum induced various histological changes in liver and testis. Treated animals with brodifacoum and ginger lead to reduction in chromosomal aberrations, significant increase in mitotic index and slight improvement in the histological change in liver and testis. Results obtained from this study demonstrated that brodifacoum has a mutagenic effect on chromosome and histopathological change in different organ (liver and testis) and the use of ginger may be a potent protective against the toxicity of brodifacoum anticoagulant rodenticide.
Brodifacoum是一种4-羟基香豆素抗凝血灭鼠剂。这表明很有可能污染了人类和农场动物的饮食,这一事实使一些科学家开始研究它们的生物学影响。生姜(Zingiber officinale)已被用作抗氧化剂和预防许多疾病的剂。本研究探讨了溴代菌素对雄性小鼠染色体、肝脏和睾丸的影响以及生姜提取物的保护作用。本研究选用成年白化雄性小鼠130只。G1组为对照组。组(G2&3)分别给予生姜提取物1.5%和3%的剂量。G4组和G5组分别给予单剂量(1/10 LD50)和单剂量(1/20 LD50)。各组(G6、7、8、9)先服用溴代香菇,再服用生姜提取物。各组(G10、11、12和13)先服用生姜提取物,然后服用溴代香菇。各组动物均处死,分析骨髓细胞染色体损伤情况。采集肝脏和睾丸标本进行组织病理学检查。溴代醌处理组小鼠染色体畸变明显增加,有丝分裂指数降低。溴代法可引起雄性小鼠肝脏和睾丸的多种组织学改变。经布罗迪法乌姆和生姜处理的动物染色体畸变减少,有丝分裂指数显著增加,肝脏和睾丸的组织学改变略有改善。本研究结果表明,溴二磷对不同器官(肝脏和睾丸)的染色体和组织病理学改变具有致突变作用,生姜可能对溴二磷抗凝血灭鼠剂的毒性有有效的保护作用。
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引用次数: 1
Effects of two local anesthetics (Mepivacaine - Articaine) on histological features of submandibular salivary gland and thyroid hormones of experimental animals 两种局麻药(甲哌卡因-阿替卡因)对实验动物下颌下唾液腺组织学特征和甲状腺激素的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220919082539
Kadria Elkasheif, Noha Awad, Ibrahim Elhalafawy, Mostufa samad, H. Ismail
Background: To examine the histological features of the hamster's submandibular salivary gland following the administration of mepivacaine and articaine as local anaesthetics Objective: The purpose of this study is to assess the anaesthetic effectiveness of both anaesthetics on thyroid hormonal levels and the histopathological changes of the gland. Materials and Methods: Twenty-four male hamsters were divided into three groups. The submandibular glands were removed and preserved in 10% phosphate buffered formalin (PH7.4) for histological investigation, while Group B and C (each of eight animals) received injections of mepivacine and articaine 2% HCl local anaesthetic at a rate of 0.05 ml per second four times after administration. In order to evaluate the amounts of the hormones triiodothyronine (T3) and thyroxin in the blood samples collected (T4) Results: Salivary gland histology using light microscopy revealed normal acini, striated ducts, and glanular ducts in group I, but atrophic changes in serous acini, a loss of acinar architecture, and congestion of inter-acinar blood capillaries in groups II and III, with the latter change being more pronounced in group III as a result of a longer induction period. Long-term use of mepivacaine and articaine medicines results in toxic effects and changes to the salivary glands that indicate stress and damage. When compared to the control, there was a statistically significant rise in T3 and TSH and a substantial drop in T4 in the experimental animals.
背景:研究甲哌卡因和阿替卡因局部麻醉后仓鼠下颌下唾液腺的组织学特征。目的:本研究的目的是评估两种麻醉剂对甲状腺激素水平和腺体组织病理学变化的麻醉效果。材料与方法:24只雄性仓鼠分为3组。取下颌下腺,保存于10%磷酸盐缓冲福尔马林(PH7.4)中进行组织学观察,B组和C组(每组8只)分别在给药后4次以0.05 ml / s的速度注射甲哌卡因和2%盐酸局部麻醉。结果:光镜下唾液腺组织学显示ⅰ组腺泡、条纹管和腺泡管正常,ⅱ组和ⅲ组腺泡萎缩,腺泡结构丧失,腺泡间毛细血管充血,ⅲ组诱导时间较长,腺泡间毛细血管充血更为明显。长期使用甲哌卡因和阿替卡因药物会导致毒性作用和唾液腺的变化,这表明压力和损害。与对照组相比,实验动物的T3和TSH有统计学意义上的显著上升,T4有显著下降。
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引用次数: 0
Do organic additives stimulate specific body weight gain and reproductive parameters of Earthworms Eisenia fetida and Aporrectodea caliginosa? 有机添加剂是否会刺激粪Eisenia fetida和Aporrectodea caliginosa蚯蚓的特定体重增加和繁殖参数?
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220527100935
Basma Assiuty, A. Khalifa, M. Ageba
Food resource availability is one of the most important factors affecting the performance and different activities of earthworms. Thus the present study focuses on testing the biological responses of two different species of earthworms one of them represents an epigeic forms Eisenia fetida and the other belongs to edaphic (Aporrectodea caliginosa) towards a mixture of organic substances of rice straw, cow dung, leaf litter, sawdust, banana peel, and kitchen waste. Specific body weight gain (SBWG) and different reproduction parameters were measured in relation to the diet quality of organic additives. Significant effects of the used organic additives on different studied parameters of both worm species could be recorded. The two earthworm species showed different responses toward the organic additives. In conclusion, it could be suggested that species specificity, worm’s ecotype, as well as food resource availability, are the main factors controlling worm biomass and different earthworm activities.
食物资源的可利用性是影响蚯蚓生产性能和不同活动的重要因素之一。因此,本研究的重点是测试两种不同种类的蚯蚓对稻草、牛粪、落叶、木屑、香蕉皮和厨余垃圾等有机物质混合物的生物学反应,其中一种蚯蚓属于附生蚯蚓,另一种属于土生蚯蚓(Aporrectodea caliginosa)。测定了添加有机添加剂与饲料质量的比增重(SBWG)和不同繁殖参数的关系。所使用的有机添加剂对两种蚯蚓的不同研究参数有显著影响。两种蚯蚓对有机添加剂的反应不同。综上所述,物种特异性、蚯蚓生态型和食物资源可得性是控制蚯蚓生物量和不同蚯蚓活动的主要因素。
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引用次数: 0
New record of Diadegma oranginator Aubert as a parasitoid of Scrobipalpa ocellatella Boyd. in Egyptian sugar beet fields. 桔斑蝶(Diadegma oranginator Aubert)的新记录。在埃及的甜菜地里。
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191209061731
K. Bazazo, A. Ibrahim
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引用次数: 2
Biochemical changes of the Liver and Kidney of Albino Mice Mus musculus Exposed to Organphosphorus insecticide Imidacloprid (Commando 30%) 有机磷杀虫剂吡虫啉(突击30%)对白化小鼠肝脏、肾脏生化的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190801083542
N. Ibrahim, Elsie Rizk, Ibrahim Shourbagy, Dina Shammari
This study planned to assess the toxicity, biochemical investigation associated with hepatotoxicity and nephrotoxicity of insecticide Imidacloprid (Commando 30%) in addition to serum cholinesterase (chE) activity in albino mice at orally administered sublethal dose of Commando 1/20 of LD50. Results revealed that The LD50 values obtained at 24, 48, 72 and 96 hours exposures and the 95% confidence limits for the imidacloprid (Commando 30%) obtained from the sigmoid curve were 495, 437, 344 and 210 mg/kg bw, respectively. the net body weight gains of the animals intoxicated with sublethal doses of Commando 30% was markedly significant decrease (P≤ 0.5). Significant reduction (P ˂ 0.05) in total protein, and significant increase in ALT, AST, ALP and LDH enzyme activity levels as well as significant decrease in kidney function (urea and creatinine) in all the experimental groups of mice. Serum Cholinestrase (ChE) activity was markedly significant decrease by Commando exposure mice at sublethal dose. The blood parameters of all tested groups, results showed that the median values of blood parameters decreased in animals treated with Commando 30% at a dose of 10.5 mg/kg body weight, indicative of anemic syndrome. In addition, there was a significant reduction in the number of erythrocytes and of hemoglobin concentration, with reduced hematocrit and increased MCV, characteristic of macrocytic anemia.
本研究拟评估杀虫剂吡虫啉(Commando 30%)对白化病小鼠的毒性及与肝毒性和肾毒性相关的生化研究,并对口服Commando 1/20 LD50亚致死剂量的白化病小鼠血清胆碱酯酶(chE)活性进行研究。结果表明,暴露24、48、72和96 h时的LD50值和30%吡虫啉(Commando 30%)的95%置信限分别为495、437、344和210 mg/kg bw。突击队员30%亚致死剂量中毒动物的净增重显著降低(P≤0.5)。各实验组小鼠总蛋白含量均显著降低(P小于0.05),ALT、AST、ALP和LDH酶活性均显著升高,肾功能(尿素和肌酐)均显著降低。亚致死剂量的Commando暴露小鼠血清胆碱酯酶(ChE)活性显著降低。结果显示,给药10.5 mg/kg体重的动物血液参数中位数下降30%,提示贫血综合征。此外,红细胞数量和血红蛋白浓度显著减少,红细胞压积降低,MCV升高,这是大细胞性贫血的特征。
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引用次数: 0
Comparitive histological, histochemical and ultrastructural studies on the ileum of African cat fish (Clarias gariepinus) and (Oreochromis niloticus) 非洲猫鱼(Clarias gariepinus)和非洲猫鱼(Oreochromis niloticus)回肠的组织学、组织化学和超微结构比较研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191119083729
Eman El Nahass, M. Elwan
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引用次数: 1
Embryonic Development of the Ramshorn Snail Helisoma duryi (Wetherby 1879) (Gastropoda, Planorbidae) from Gharbia Province, Egypt 埃及加尔比亚省Ramshorn蜗牛Helisoma duryi (Wetherby 1879)(腹足目,Planorbidae)的胚胎发育
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220519025006
S. Eissa, Hoda K. Salem, Manar A. Kandeil, S. Hassan
Embryonic development of the freshwater planorbid snail, Helisoma duryi, reared under laboratory controlled conditions was described in detail. The morphological characters and the time course of embryonic stages are presented. H. duryi generally lay egg masses at night or in the early morning hours. The egg type of H. duryi is isolecithal, and the cleavage is spiral holoblastic. After early cleavages followed by the appearance of the blastula, gastrula, and larval (trochophore, veliger, veliconch) stages, the fully developed young snail start hatched on the eighth day. Many unique developmental features in studied snail were observed and discussed.
详细描述了在实验室控制条件下饲养的淡水扁螺(Helisoma duryi)胚胎发育。介绍了其胚期的形态特征和时间进程。杜氏夜蛾一般在夜间或清晨产卵。杜氏双歧杆菌卵型为等卵型,卵裂为螺旋形全裂。经过早期的卵裂,随后出现囊胚,原肠胚和幼虫(trochophore, veliger, veliconch)阶段,完全发育的小蜗牛开始在第八天孵化。观察并讨论了所研究蜗牛的许多独特发育特征。
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引用次数: 0
Scanning electron microscope studies of the dorsal lingual surface of some reptilian species 某些爬行动物舌背表面的扫描电子显微镜研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191024054829
Siham B. Salem, D. Mies, A. Eldeen, Ramadan M. Kandyel
{"title":"Scanning electron microscope studies of the dorsal lingual surface of some reptilian species","authors":"Siham B. Salem, D. Mies, A. Eldeen, Ramadan M. Kandyel","doi":"10.5455/egysebz.20191024054829","DOIUrl":"https://doi.org/10.5455/egysebz.20191024054829","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133375522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)
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