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In silico Characterization of Biofilm-Associated Protein (Bap) Identified in a Multi-drug Resistant Acinetobacter baumannii Clinical Isolate 多药耐药鲍曼不动杆菌临床分离物生物膜相关蛋白(Bap)的计算机表征
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.210
Prof. M.R Shakibaie
dihedral angles, including the phi (φ) and psi (ψ) and backbone conformation using the PROCHECK module of the PDB Sum server was performed. The domains and key amino acids involved in protein structure were studied by the Pfam and Interpro softwares. Results: Analysis of the amino acid content of the Bap protein revealed the absence of Arg and Cys in the protein structure. Our Bap protein exhibited ~99.6% identity with other Bap sequences in the GenBank database. Stereochemical simulation identified 19 antiparallel β-sheets with two small α-helices. The N-terminal of Bap protein formed oligomers that mediate cellular adhesion. Conclusion: This study adds considerable information about Bap protein 3D structure, its conformation, domain analysis, and amino acids involved in cellular attachment.
利用PDB Sum服务器的PROCHECK模块进行二面角,包括φ (φ)和psi (ψ)和主干构象的计算。利用Pfam和Interpro软件对蛋白质结构域和关键氨基酸进行了研究。结果:对Bap蛋白的氨基酸含量分析显示,该蛋白结构中不含Arg和Cys。我们的Bap蛋白与GenBank数据库中其他Bap序列的同源性为99.6%。立体化学模拟鉴定出19个具有两个小α-螺旋的反平行β-片。Bap蛋白的n端形成低聚物,介导细胞粘附。结论:本研究为Bap蛋白的三维结构、构象、结构域分析和参与细胞附着的氨基酸提供了大量信息。
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引用次数: 0
Burkholderia cepacia Complex, an Emerging Nosocomial Pathogen at Health Care Facilities in Sebha, Libya 洋葱伯克霍尔德菌复合体,利比亚塞卜哈卫生保健设施中一种新出现的医院病原体
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.178
Shamsi Saad Shamsi, Abdelkader A. Elzen, Khadija M. Ahmad
Burkholderia cepacia Complex, an Emerging Nosocomial Pathogen at Health Care Facilities in Sebha, Libya Shamsi Saad Shamsi , Abdelkader A. Elzen , Khadija M. Ahmad 4 Department of Plant Science, Microbiology Program, Research laboratory, Faculty of Science, Sebha University; Department of Microbiology, Faculty of Science, Sebha University, Sebha, Libya; Department of Microbiology, Faculty of Medicine, Sebha University, Sebha, Libya; Department of Microbiology, Sebha medical center, Sebha
4塞卜哈大学科学学院植物科学系,微生物学项目,研究实验室塞卜哈大学理学院微生物系,利比亚塞卜哈;塞卜哈大学医学院微生物系,利比亚塞卜哈;塞卜哈医疗中心微生物科,塞卜哈
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引用次数: 0
The quandary over Antiviral Therapy for a COVID-19 Patient with Glucose-6-Phosphate Dehydrogenase Deficiency, Hypertension, and Resolved Hepatitis B Infection: A Case Report 1例糖-6-磷酸脱氢酶缺乏症合并高血压合并乙型肝炎治愈的COVID-19患者抗病毒治疗的困境
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.221
Prakash Gyandev Gondode, A. Garg, S. Sahoo, Ankit Sharma
The quandary over Antiviral Therapy for a COVID-19 Patient with Glucose-6Phosphate Dehydrogenase Deficiency, Hypertension, and Resolved Hepatitis B Infection: A Case Report Prakash Gyandev Gondode , Ankita Garg , Satyajeet Sahoo , Ankit Sharma Department of Anesthesiology, All India Institute of Medical Sciences, Nagpur, India; Department of Anaesthesiology, Dr Babasaheb Ambedkar Hospital, Rohini, Delhi, India; Department of Pulmonology, All India Institute of Medical Sciences, Nagpur, India; Department of Onco-Anesthesia, IRCH, All India Institute of Medical Sciences, Delhi, India
COVID-19合并葡萄糖-6磷酸脱氢酶缺乏症、高血压和已解决的乙型肝炎感染的抗病毒治疗困境:Prakash Gyandev Gondode, Ankita Garg, Satyajeet Sahoo, Ankit Sharma病例报告印度德里罗希尼Babasaheb Ambedkar医院麻醉科;全印度医学科学研究所肺科,印度那格浦尔;全印度医学科学研究所肿瘤麻醉科,印度德里
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引用次数: 0
High Frequency of Salivirus in Pediatric Patients with Acute Gastroenteritis 小儿急性胃肠炎患者唾液病毒的高发
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.191
Saber Basiri, B. Khalesi, S. D. Mousavi-Nasab, Zahra Ivani, M. Ghaderi
High Frequency of Salivirus in Pediatric Patients with Acute Gastroenteritis Saber Basiri , Bahman Khalesi , Seyed Dawood Mousavi-Nasab , Zahra Ivani , Mostafa Ghaderi Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran; Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran; Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran; Department of Science, Razi Vaccine and Serum Research Institute
小儿急性胃肠炎患者唾液病毒的高发性研究伊朗卡拉伊农业研究教育和推广组织(AREEO)拉兹疫苗和血清研究所家禽病毒疫苗研究和生产部;伊朗巴斯德研究所生产和研究中心研发部,伊朗德黑兰;拉兹疫苗与血清研究所科学部
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引用次数: 0
Tocilizumab in Post Hematopoietic Stem Cell Transplant COVID-19: A Pediatric Case Report 托珠单抗治疗造血干细胞移植后COVID-19:一份儿科病例报告
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.225
T. Rostami, N. Alijani, A. Kiumarsi, S. Rad, S. Mirhoseini, Mohammadreza Rostami, A. Hesabi, S. Mousavi, S. Mousavi
Tocilizumab in Post Hematopoietic Stem Cell Transplant COVID-19: A Pediatric Case Report Tahereh Rostami , Neda Alijani , Azadeh Kiumarsi , Soroush Rad , Seied Amirhosein Mirhoseini , Mohammadreza Rostami , Amir Hesabi , Seyed Ali Mousavi , Seied Asadollah Mousavi Department of Pediatric Cell Therapy, Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran; Department of Infectious Diseases, Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran; Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran
Tahereh Rostami, Neda Alijani, Azadeh Kiumarsi, Soroush Rad, Seied Amirhosein Mirhoseini, Mohammadreza Rostami, Amir Hesabi, Seyed Ali Mousavi, Seied Asadollah Mousavi儿科细胞治疗系,肿瘤学,血液学和细胞治疗研究所,伊朗德黑兰;德黑兰医科大学肿瘤学、血液学和细胞治疗研究所传染病系,伊朗德黑兰;德黑兰医科大学肿瘤学、血液学和细胞治疗研究所,伊朗德黑兰
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引用次数: 0
Molecular characterization of Extended Spectrum Beta lactamases producing bacteria causing Chronic Suppurative Otitis Media in South India 在南印度引起慢性化脓性中耳炎的广谱β内酰胺酶产生细菌的分子特征
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.196
A. Kavitha, R. Katragadda, L. Vajravelu, T. Ravinder
© The Author(s) Introduction: Chronic suppurative otitis media (CSOM) is one of the most common middle ear infections leading to extra and intracranial complications if not diagnosed promptly. Early identification and detection of the etiological agents and antibiotic susceptibility patterns assist in preventing complications. Methods: Two hundred twelve ear swabs were collected using sterile cotton swabs. Direct gram staining was done and then inoculated into blood, MacConkey, and Nutrient agar. Bacterial isolates were identified using conventional methods. According to CLSI guidelines, Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method. Minimum inhibitory concentration (MIC) was performed by the agar dilution method. Extended-spectrum beta-lactamases producing bacteria were detected by the phenotypic confirmatory test and then corroborated by uniplex PCR. Results: Out of 212 samples, 157 samples (74.06%) were culture-positive for bacteria. The isolated bacteria included Pseudomonas aeruginosa (46.24%), Staphylococcus aureus (26.59%), Klebsiella pneumoniae (14.45%), coagulasenegative Staphylococcus aureus (5.20%), Proteus mirabilis (4.05%), Enterococcus faecalis (2.89%), and Escherichia coli (0.58%). The P. aeruginosa isolates showed 96.25% and 95% susceptibility to amikacin and ofloxacin, respectively. All Gram-negative bacilli isolates were 100% sensitivite to imipenem. Ten (30.30%) isolates were ESBL producers with the CTX-M-14 gene detected in most of them. Conclusion: Our study found that P. aeruginosa was the most common isolated pathogen bacteria. Knowledge of CSOM causing bacteria and their susceptibility to antibiotics would help choose an appropriate treatment, thereby preventing antibiotic resistance and complications in these cases.
©作者简介:慢性化脓性中耳炎(CSOM)是最常见的中耳感染之一,如果不及时诊断,可导致颅内外并发症。早期识别和检测病原和抗生素敏感性模式有助于预防并发症。方法:采用无菌棉签采集耳拭子212份。直接革兰氏染色,然后接种到血液、麦康基和营养琼脂中。采用常规方法对分离的细菌进行鉴定。根据CLSI指南,采用Kirby-Bauer圆盘扩散法进行抗生素敏感性试验。琼脂稀释法测定最低抑菌浓度(MIC)。采用表型验证试验检测广谱β -内酰胺酶产生菌,并用单链PCR进行验证。结果:212份样品中细菌培养阳性157份(74.06%)。其中,铜绿假单胞菌(46.24%)、金黄色葡萄球菌(26.59%)、肺炎克雷伯菌(14.45%)、凝固阴性金黄色葡萄球菌(5.20%)、奇异变形杆菌(4.05%)、粪肠球菌(2.89%)、大肠杆菌(0.58%)。铜绿假单胞菌对阿米卡星和氧氟沙星的敏感性分别为96.25%和95%。所有革兰氏阴性杆菌对亚胺培南均100%敏感。10株(30.30%)分离株为ESBL产生菌,大部分分离株检测到CTX-M-14基因。结论:本研究发现铜绿假单胞菌是最常见的分离病原菌。了解引起CSOM的细菌及其对抗生素的敏感性有助于选择适当的治疗方法,从而预防这些病例的抗生素耐药性和并发症。
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引用次数: 1
Identification of Carbapenemase within Class 1 Integron Structure in Intrinsically Colistin-resistant Enterobacteriaceae 内在耐粘菌素肠杆菌科1类整合子结构碳青霉烯酶的鉴定
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.203
N. Bolourchi, O. Azizi, M. Rohani, Soha Seyyedi Abhari, Armaghan Soltani Shirazi, F. Badmasti
Identification of Carbapenemase within Class 1 Integron Structure in Intrinsically Colistin-resistant Enterobacteriaceae Negin Bolourchi , Omid Azizi , Mahdi Rohani , Soha Seyyedi Abhari , Armaghan Soltani Shirazi , Farzad Badmasti * Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran; Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences; Microbiology Research Center (MRC), Pasteur Institute of Iran
neginbolourchi, Omid Azizi, Mahdi Rohani, Soha Seyyedi Abhari, Armaghan Soltani Shirazi, Farzad Badmasti *伊朗巴斯德研究所细菌学系,伊朗德黑兰;Torbat Heydariyeh医科大学健康科学研究中心;伊朗巴斯德研究所微生物学研究中心
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引用次数: 0
Expression Analysis of MicroRNAs, miR-20a, miR-30a, miR-210, and miR-874 in Helicobacter pylori-infected patients with or without Gastric Cancer microrna、miR-20a、miR-30a、miR-210和miR-874在合并或非胃癌幽门螺杆菌感染患者中的表达分析
Pub Date : 2021-12-01 DOI: 10.52547/jommid.9.4.185
Saba Hariri Monfared, Mina Yaghoub Kazemi, Fahimeh Nemati Mansoor, Mahnaz Mohammadi
Saba Hariri Monfared , Mina Yaghoub Kazemi , Fahimeh Nemati Mansour , Mahnaz Mohammadi M.Sc, Department of Biotechnology and Microbiology, Medicine Branch, Islamic Azad University, Tehran, Iran; Associate Professor, Department of Biotechnology, Medicine Branch, Islamic Azad University, Tehran, Iran; Associate Professor, Department of Biology, Faculty of Biological Sciences, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
伊朗德黑兰伊斯兰阿扎德大学生物技术与微生物学系,Mina Yaghoub Kazemi, Fahimeh Nemati Mansour, Mahnaz Mohammadi理学硕士;伊朗德黑兰伊斯兰阿扎德大学医学部生物技术系副教授;伊朗伊斯兰阿扎德大学伊斯兰沙赫尔分校生物科学学院生物系副教授
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引用次数: 0
Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine 巴勒斯坦加沙地带患者分离的广谱β -内酰胺酶(ESBLs)产生大肠杆菌和肺炎克雷伯菌的频率和抗生素耐药性
Pub Date : 2021-09-01 DOI: 10.52547/jommid.9.3.133
G. Tayh, N. A. Al Laham, Imene Fhoula, Nahed Abedelateef, Mohamed D El-laham, Abed Elkader Elottol, K. Ben Slama
Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine Ghassan Tayh 7* , Nahed Al Laham , Imene Fhoula , Nahed Abedelateef , Mohamed El-Laham , Abed Elkader Elottol , Karim Ben Slama 6 Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie; Department of Laboratory Medicine, Faculty of Applied Medical Sciences, AlAzhar University-Gaza, Palestine; Department of Biological Science, Al-Aqsa University –Gaza, Palestine; Department of Biological Science and Medical Technology, Israa University – Gaza, Palestine; Medical Science Department, University College of Science and Technology Gaza, Palestine; 6 Institut Supérieur des Sciences Biologiques Appliquées de TunisUniversité de Tunis El Manar, 1006 Tunis, Tunisie;Service de Microbiologie et d’Immunologie, Ecole Nationale de Médecine Vétérinaire, Univ. Manouba, Sidi Thabet, Tunisie
巴勒斯坦加沙地带患者中产生大肠杆菌和肺炎克莱伯菌的广谱β -内酰胺酶(ESBLs)的频率和抗生素耐药性Ghassan Tayh 7*, Nahed Al Laham, Imene Fhoula, Nahed Abedelateef, Mohamed El-Laham, Abed Elkader Elottol, Karim Ben Slama 6微生物和生物化学化学活性实验室,突尼斯大学El Manar,突尼斯,2092;巴勒斯坦加沙阿拉资哈尔大学应用医学学院检验医学系;巴勒斯坦加沙阿克萨大学生物科学系;以色列大学生物科学和医学技术系-巴勒斯坦加沙;巴勒斯坦加沙科技大学医学院;6突尼斯大学埃尔马纳尔校区,突尼斯,1006;马努巴大学,突尼斯,西迪塔贝特,突尼斯,突尼斯,马努巴,突尼斯,西迪塔贝特,突尼斯,马努巴,突尼斯,西迪塔贝特,突尼斯,马努巴,突尼斯,西迪塔贝特
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引用次数: 3
Global Variability of V3 Loop Tetrapeptide Motif: a Concern for HIV-1 Neutralizing Antibodies-based Vaccine Design and Antiretroviral Therapy V3环四肽基序的全局变异性:基于HIV-1中和抗体的疫苗设计和抗逆转录病毒治疗的关注
Pub Date : 2021-09-01 DOI: 10.52547/jommid.9.3.108
L. R. Lopes, Antônio Carlos da Silva Júnior, Paulo Bandiera-Paiva, J. Casseb
Global Variability of V3 Loop Tetrapeptide Motif: a Concern for HIV-1 Neutralizing Antibodies-based Vaccine Design and Antiretroviral Therapy Luciano Rodrigo Lopes , Antonio Carlos da Silva Junior , Paulo Bandiera-Paiva , Jorge Casseb Bioinformatics and Bio-Data Science Division, Health Informatics Department, Universidade Federal de São Paulo– UNIFESP, São Paulo, SP, Brazil; Institute of Tropical Medicine of Sao Paulo University of Sao Paulo, Laboratory of Medical Investigation LIM-56 / Faculty of Medicine –USP
V3环四肽基序的全球变异:基于HIV-1中和抗体的疫苗设计和抗逆转录病毒治疗的关注Luciano Rodrigo Lopes, Antonio Carlos da Silva Junior, Paulo Bandiera-Paiva, Jorge Casseb卫生信息部生物信息学和生物数据科学部,圣保罗联邦大学- UNIFESP,圣保罗,SP,巴西;圣保罗大学热带医学研究所,医学调查实验室LIM-56 /医学院
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引用次数: 0
期刊
Journal of Medical Microbiology and Infectious Diseases
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