M. Ja’afaru, Abubakar Gaure, J. U Ewansiha, O. Adeyemo
ABSTRACT
摘要
{"title":"Prevalence of Extended Spectrum β-Lactamases-Producing Escherichia coli Isolated from Clinical Samples and Their Antibiotic Resistance Pattern","authors":"M. Ja’afaru, Abubakar Gaure, J. U Ewansiha, O. Adeyemo","doi":"10.52547/jommid.11.1.34","DOIUrl":"https://doi.org/10.52547/jommid.11.1.34","url":null,"abstract":"ABSTRACT","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82129500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Cholera is a highly contagious disease that causes severe diarrhea and dehydration. This study investigated podophyllotoxin, deoxypodophyllotoxin, and ursolic acid as inhibitors of tcpA , ompW, and ctxB genes in Vibrio cholerae . Methods: We obtained the crystallized structure of podophyllotoxin, deoxypodophyllotoxin, and ursolic acid from the PubChem database for use as a ligand. The mm 2 method in Chem3D v20.1.1.125 was used to optimize the structure of the ligands. We used AutodackVina v.1.2.0 to evaluate the ligands as inhibitors against the active site of the tcpA , ompW , and ctxB proteins. The output results were analyzed and assessed by BIOVIA Discovery Studio 2016 V16.1.0 X64. Results: The reported affinities ranged from -6.8 and -8.7 kcal/mol. The highest diversity of links was found in tcpA and ctxB . Hydrogen bonds were established with Threonine (91, 111), Glycine (113, 114, 94), and Alanine (92) of tcpA , indicating the effectiveness of ligands against tcpA . The ligands podophyllotoxin, deoxypodophyllotoxin, and ursolic acid showed a variety of hydrogen bonds against ompW and ctxB , respectively, with Arginine, Isoleucine, Histidine, Glycine, and Glutamine. These results demonstrate the excellent inhibitory effects of the ligands against Vibrio cholerae . Conclusion: Vibrio cholerae plays a crucial role in causing pandemic cholera in humans. The predicted conformations of the ligands in this study showed that podophyllotoxin and deoxypodophyllotoxin have higher inhibitory potential than ursolic acid. Therefore, podophyllotoxin and deoxypodophyllotoxin can be potential agents for further research in developing Anti-Vibrio cholerae drugs.
霍乱是一种高度传染性疾病，可引起严重腹泻和脱水。本研究研究了鬼臼毒素、脱氧鬼臼毒素和熊果酸作为霍乱弧菌tcpA、ompW和ctxB基因抑制剂的作用。方法:从PubChem数据库中获得鬼臼毒素、脱氧鬼臼毒素和熊果酸的结晶结构，作为配体使用。采用Chem3D软件v20.1.1.125中的mm2方法对配体结构进行优化。我们使用AutodackVina v.1.2.0来评估这些配体作为抑制tcpA、ompW和ctxB蛋白活性位点的抑制剂。输出结果由BIOVIA Discovery Studio 2016 V16.1.0 X64进行分析和评估。结果:报告的亲和力范围为-6.8和-8.7 kcal/mol。在tcpA和ctxB中发现了最高的链接多样性。与tcpA的苏氨酸(91,111)、甘氨酸(113,114,94)和丙氨酸(92)建立了氢键，表明配体对tcpA的有效性。配体鬼臼毒素、脱氧鬼臼毒素和熊果酸分别与精氨酸、异亮氨酸、组氨酸、甘氨酸和谷氨酰胺对ompW和ctxB表现出不同的氢键。这些结果表明该配体对霍乱弧菌具有良好的抑制作用。结论:霍乱弧菌在引起人类大流行霍乱中起重要作用。本研究预测的配体构象表明，鬼臼毒素和脱氧鬼臼毒素比熊果酸具有更高的抑制潜力。因此，鬼臼毒素和脱氧鬼臼毒素可能是开发抗霍乱弧菌药物的潜在药物。
{"title":"Podophyllotoxin, Deoxypodophyllotoxin and Ursolic Acid as Potential Inhibitors of tcpA, ompW, and ctxB Genes in Vibrio cholerae: An in-Silico Study","authors":"Yasin SarveAhrabi, Ovis Rostamiyan, Sarina Nejati Khoei","doi":"10.52547/jommid.11.1.41","DOIUrl":"https://doi.org/10.52547/jommid.11.1.41","url":null,"abstract":"Introduction: Cholera is a highly contagious disease that causes severe diarrhea and dehydration. This study investigated podophyllotoxin, deoxypodophyllotoxin, and ursolic acid as inhibitors of tcpA , ompW, and ctxB genes in Vibrio cholerae . Methods: We obtained the crystallized structure of podophyllotoxin, deoxypodophyllotoxin, and ursolic acid from the PubChem database for use as a ligand. The mm 2 method in Chem3D v20.1.1.125 was used to optimize the structure of the ligands. We used AutodackVina v.1.2.0 to evaluate the ligands as inhibitors against the active site of the tcpA , ompW , and ctxB proteins. The output results were analyzed and assessed by BIOVIA Discovery Studio 2016 V16.1.0 X64. Results: The reported affinities ranged from -6.8 and -8.7 kcal/mol. The highest diversity of links was found in tcpA and ctxB . Hydrogen bonds were established with Threonine (91, 111), Glycine (113, 114, 94), and Alanine (92) of tcpA , indicating the effectiveness of ligands against tcpA . The ligands podophyllotoxin, deoxypodophyllotoxin, and ursolic acid showed a variety of hydrogen bonds against ompW and ctxB , respectively, with Arginine, Isoleucine, Histidine, Glycine, and Glutamine. These results demonstrate the excellent inhibitory effects of the ligands against Vibrio cholerae . Conclusion: Vibrio cholerae plays a crucial role in causing pandemic cholera in humans. The predicted conformations of the ligands in this study showed that podophyllotoxin and deoxypodophyllotoxin have higher inhibitory potential than ursolic acid. Therefore, podophyllotoxin and deoxypodophyllotoxin can be potential agents for further research in developing Anti-Vibrio cholerae drugs.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89089683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sameer Abdul Samad, Hafeedha Kallarakkal, N. Basheer
Intraventricular Administration of Cefepime for the Treatment of Meningitis due to Pseudomonas aeruginosa Sameer Abdul Samad , Hafeedha Kallarakkal , Noufal Basheer Department of Infectious Diseases, Aster Malabar Institute of Medical Sciences, Calicut, India; Department of Clinical Pharmacy, Aster Malabar Institute of Medical Sciences, Calicut, India; Department of Neurosurgery, Aster Malabar Institute of Medical Sciences, Calicut, India
脑室内给药头孢吡肟治疗铜绿假单胞菌所致脑膜炎Sameer Abdul Samad, Hafeedha Kallarakkal, Noufal Basheer感染性疾病部，Aster Malabar医学科学研究所，印度卡利卡特;印度卡利卡特阿斯特马拉巴尔医学研究所临床药学系;印度卡利卡特阿斯特马拉巴尔医学科学研究所神经外科
{"title":"Intraventricular Administration of Cefepime for the Treatment of Meningitis due to Pseudomonas aeruginosa","authors":"Sameer Abdul Samad, Hafeedha Kallarakkal, N. Basheer","doi":"10.52547/jommid.11.1.60","DOIUrl":"https://doi.org/10.52547/jommid.11.1.60","url":null,"abstract":"Intraventricular Administration of Cefepime for the Treatment of Meningitis due to Pseudomonas aeruginosa Sameer Abdul Samad , Hafeedha Kallarakkal , Noufal Basheer Department of Infectious Diseases, Aster Malabar Institute of Medical Sciences, Calicut, India; Department of Clinical Pharmacy, Aster Malabar Institute of Medical Sciences, Calicut, India; Department of Neurosurgery, Aster Malabar Institute of Medical Sciences, Calicut, India","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75651975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Ndong Mba, Hilaire Moundounga Kenguele, Ulrick Nzamba, A. B. Pambo-Pambo, Romial Zang Mintsa, P. Mickala
Introduction
介绍
{"title":"Prevalence of Salmonella enterica Subspecies enterica Serovar Typhi (Salmonella typhi) Infection in Febrile Patients at the Sino-Gabonese Friendship Hospital in Franceville: A Two-Year Retrospective Study in South East Gabon","authors":"T. Ndong Mba, Hilaire Moundounga Kenguele, Ulrick Nzamba, A. B. Pambo-Pambo, Romial Zang Mintsa, P. Mickala","doi":"10.52547/jommid.11.1.20","DOIUrl":"https://doi.org/10.52547/jommid.11.1.20","url":null,"abstract":"Introduction","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74924550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sabereh Ahmadzadeh, M. Sedighi, Amin Torkashvand, Pejman Hashemzadeh, F. Shafiei, M. Torkashvand, A. Hasan-Zadeh
ABSTRACT
摘要
{"title":"Pathological Effects of COVID-19 on Body Organs","authors":"Sabereh Ahmadzadeh, M. Sedighi, Amin Torkashvand, Pejman Hashemzadeh, F. Shafiei, M. Torkashvand, A. Hasan-Zadeh","doi":"10.52547/jommid.11.1.1","DOIUrl":"https://doi.org/10.52547/jommid.11.1.1","url":null,"abstract":"ABSTRACT","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88905693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Sreekanth, Amarendra Goviundu, Dhanalakshmi A, Manikonda Rajini
HIV-positive
艾滋病毒阳性
{"title":"A Recent Profile of HIV-Positive Patients Attending an Integrated Testing and Counseling Centre (ICTC) at a Tertiary Care Center in Bangalore, India","authors":"B. Sreekanth, Amarendra Goviundu, Dhanalakshmi A, Manikonda Rajini","doi":"10.52547/jommid.11.1.49","DOIUrl":"https://doi.org/10.52547/jommid.11.1.49","url":null,"abstract":"HIV-positive","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78100311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Arbabi, Aimohammad Bakhshi, Hossein Hooshyar, R. Ghasemikhah, Mahdi Delavari, M. Sehat
length, were measured in 70 worm isolates. The data were analyzed using the ANOVA test in SPSS 18 software. Results: Of 284 livestock (130 goats and 154 sheep) examined, 26 (9/15%) were infected with Trichostrongylus. The prevalence of infection in goats and sheep was 12.3% and 6.5%, respectively. The most frequent species were Trichostrongylus colubriformis (48.7%), followed by Trichostrongylus vitrinus (25.7%). Trichostrongylus capricula and Trichostrongylus probolurus had an incidence of 12.8%. T. probolurus showed a higher length of spicule and gubernaculum, while T. vitrinus showed a wider copulatory bursa compared to the other species ( P <0.001). Conclusion: The prevalence of Trichostrongylus infection in this region was remarkable. Morphometric and morphological methods are practical tools in differentiating
{"title":"Prevalence and Morphometric Comparison of Trichostrongylus spp. among Sheep and Goats from Kashan Abattoir, Central Iran","authors":"M. Arbabi, Aimohammad Bakhshi, Hossein Hooshyar, R. Ghasemikhah, Mahdi Delavari, M. Sehat","doi":"10.52547/jommid.11.1.28","DOIUrl":"https://doi.org/10.52547/jommid.11.1.28","url":null,"abstract":"length, were measured in 70 worm isolates. The data were analyzed using the ANOVA test in SPSS 18 software. Results: Of 284 livestock (130 goats and 154 sheep) examined, 26 (9/15%) were infected with Trichostrongylus. The prevalence of infection in goats and sheep was 12.3% and 6.5%, respectively. The most frequent species were Trichostrongylus colubriformis (48.7%), followed by Trichostrongylus vitrinus (25.7%). Trichostrongylus capricula and Trichostrongylus probolurus had an incidence of 12.8%. T. probolurus showed a higher length of spicule and gubernaculum, while T. vitrinus showed a wider copulatory bursa compared to the other species ( P <0.001). Conclusion: The prevalence of Trichostrongylus infection in this region was remarkable. Morphometric and morphological methods are practical tools in differentiating","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82992680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exacerbation of Activated Latent Tuberculosis Following COVID-19: A Case Report","authors":"Lautika Sonkar, Rampal Singh, Jagdamba Sharan, Deepika Verma","doi":"10.52547/jommid.10.4.204","DOIUrl":"https://doi.org/10.52547/jommid.10.4.204","url":null,"abstract":"","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"47 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72544346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52547/jommid.10.4.153
Shalini Shukla, K. Kamini, B. Gupta, S. Bahadur, S. Kalhan, Madhuvan Gupta
Introduction: COVID-19 infection can be complicated by coagulation derangement and a high risk of thromboembolic episodes. Our study aimedto investigate coagulation parameters in COVID-19 patients and their correlation with clinical severity. Methods: We analyzed coagulation parameters PT, APTT, D-Dimer, and Fibrinogen in 98 RT-PCR-confirmed COVID-19 patients admitted to the Government Institute of Medical Sciences, Gautam Buddha Nagar, Uttar Pradesh, India. Results: This study involved 69 males (70.50%), and 29 (29.5%) were females. The mortality rate was 6.12% (n= 06). Forty-six patients (46.94%) had comorbidities. Thirty-four patients had elevated PT, and 7 had high APTT, whereas D-dimer and fibrinogen levels were raised in 68 and 61 patients, respectively. Among all four parameters, D-Dimer levels were significantly associated with disease severity. Conclusion: Derangement of D-dimer levels is significantly associated with disease severity in COVID-19 infection.
{"title":"Parameters of Coagulation in COVID-19 Patients: A Correlation with Clinical Severity","authors":"Shalini Shukla, K. Kamini, B. Gupta, S. Bahadur, S. Kalhan, Madhuvan Gupta","doi":"10.52547/jommid.10.4.153","DOIUrl":"https://doi.org/10.52547/jommid.10.4.153","url":null,"abstract":"Introduction: COVID-19 infection can be complicated by coagulation derangement and a high risk of thromboembolic episodes. Our study aimedto investigate coagulation parameters in COVID-19 patients and their correlation with clinical severity. Methods: We analyzed coagulation parameters PT, APTT, D-Dimer, and Fibrinogen in 98 RT-PCR-confirmed COVID-19 patients admitted to the Government Institute of Medical Sciences, Gautam Buddha Nagar, Uttar Pradesh, India. Results: This study involved 69 males (70.50%), and 29 (29.5%) were females. The mortality rate was 6.12% (n= 06). Forty-six patients (46.94%) had comorbidities. Thirty-four patients had elevated PT, and 7 had high APTT, whereas D-dimer and fibrinogen levels were raised in 68 and 61 patients, respectively. Among all four parameters, D-Dimer levels were significantly associated with disease severity. Conclusion: Derangement of D-dimer levels is significantly associated with disease severity in COVID-19 infection.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73871877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：