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Evaluation and Optimization of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Salmonella enterica serovar Typhi Isolated from Food Samples 环介导等温扩增(LAMP)法检测食品中伤寒沙门氏菌血清型的评价与优化
Q4 Medicine Pub Date : 2020-11-01 DOI: 10.29252/IEM.6.4.259
F. Foroohi, H. Zamani, Sajjad Yazdan setad
CITATION LINKS A R T I C L E I N F O Article Type Original Research Authors Fatemeh Forohi, PhD1* Hadi Zamani, PhD1 Sajjad Yazdansetad, PhD2 How to cite this article Forohi F., Zamani H., Yazdansetad S. Evaluation and Optimization of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Salmonella enterica serovar Typhi Isolated from Food Samples. Infection Epidemiology and Microbiology. 2020;6(4): 259-267 1Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran 2Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran * Correspondence Address: Department of Microbiology, Faculty of Basic Sciences, Shahre-Qods-Branch, Islamic Azad University, Tehran, Iran. f_foroohi@ymail.com Article History Received: July 21 ,2020 Accepted: October 15 ,2020 Published: November 19 ,2020 [1] Bhandari DP. Characterization of flagellin antigen using a panel of ...[2] Babar A, Butt SJ, Ullah S. A review of prognostic models on diagnosis and ... [3] Olobatoke R. Public health burden of non-typhoidal Salmonella strains in sub-Saharan ... [4] Yang Q, Domesle KJ, Wang F, Ge B. Rapid detection of Salmonella in food and feed ... [5] Foddai AC, Grant IR. Methods for detection of viable foodborne pathogens: Current state-of-art ... [6] Zhai L, Liu H, Chen Q, Lu Z, Zhang C, Lv F, et al. Development of a real-time nucleic acid ... [7] Linh Quyen T, Nordentoft S, Chidambara Vinayaka A, Ngo TA, Engelsmenn P, Sun Y, et al. A sensitive, specific, and ... [8] Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated ... [9] HaraKudo Y, Yoshino M, Kojima T, Ikedo M. Loop-mediated isothermal amplification for ... [10] Havelaar A, Grace D, Wu F. Foodborne diseases from dairy products in developing ...[11] Shahbazi S, Karam MRA, Habibi M, Talebi A, Bouzari S. Distribution of ... [12] Nair A, Balasaravanan T, Malik SS, Mohan V, Kumar M, Vergis J, et al. Isolation and ... [13] Köchl S, Niederstätter H, Parson W. DNA extraction and quantitation of forensic ... [14] Vickers NJ. Animal communication: When I’m calling you, will you answer ... [15] Zhang G, Brown EW, González-Escalona N. Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for ...[16] Srisawat M, Panbangred W. Efficient and specific detection of Salmonella in food ... [17] Yang Q, Domesle KJ, Ge B. Loop-mediated isothermal amplification for Salmonella detection ... [18] Ravan H, Amandadi M. A rapid and specific detection of pathogenic serovar Salmonella ... [19] Moganedi K, Goyvaerts E, Venter SN, Sibara M. Optimisation of the PCR-invA primers for ... [20] Techathuvanan C, Draughon FA, D’Souza DH. Loop-mediated isothermal amplification (LAMP) for ... [21] Zhao X, Wang L, Chu J, Li Y, Li Y, Xu Z, et al. Development and application of a rapid and simple ... [22] Zhang Y, Shan X, Shi L, Lu X, Tang S, Wang Y, et al.
引用本文A R TI C L E IN F O文章类型主要作者Fatemeh Forohi, PhD1* Hadi Zamani, PhD1 Sajjad yazdansead, PhD2本文引用本文Forohi F, Zamani H, yazdansead S.环介导等温扩增(LAMP)方法检测食品样品中伤寒沙门氏菌血清型的评价与优化。1伊朗伊斯兰阿扎德大学Shahr-e-Qods分校微生物学系,伊朗德黑兰2伊朗戈勒斯坦医科大学实验室科学研究中心,伊朗戈尔根*通讯地址:伊朗德黑兰伊斯兰阿扎德大学Shahr-e-Qods分校基础科学学院微生物学系,伊朗德黑兰f_foroohi@ymail.com文章历史收稿日期:2020年7月21日收稿日期:2020年10月15日发布日期:2020年11月19日[1]Bhandari DP。鞭毛蛋白抗原的鉴定[2]刘建军,刘建军,刘建军,等。肿瘤预后模型的研究进展[j]。[3]王晓明,王晓明,王晓明,等。非伤寒沙门氏菌在撒哈拉以南地区的公共卫生负担。[4]杨强,王峰,葛波。食品和饲料中沙门氏菌的快速检测[j]。[5]李志强,李志强。食源性致病菌的检测方法:最新进展…[6]翟磊,刘辉,陈强,卢忠,张超,吕峰,等。研制实时核酸…[7]林春燕,孙勇,孙勇。一个敏感的,具体的,而且…[8]张建军,张建军,张建军,等。Loop-mediated……[9]张建军,张建军,张建军,等。[10]张晓明,王晓明,王晓明,等。食源性疾病的研究进展[11]Shahbazi S, Karam MRA, Habibi M, Talebi A, Bouzari S.…[12]李建军,李建军,李建军,等。隔离和…[13] Köchl S, Niederstätter H, Parson W. DNA提取与定量鉴定…[14]王志强,王志强。动物交流:当我叫你的时候,你会回答吗?[15]张刚,黄文华,González-Escalona .实时荧光定量PCR、逆转录酶实时荧光定量PCR、环介导等温扩增与FDA传统微生物学方法的比较[16]李建军,李建军。食品中沙门氏菌的高效特异性检测方法研究。[17]杨青,郭建军,葛波。环介导等温扩增检测沙门氏菌[j]。[18]王晓明,王晓明,王晓明,等。血清型沙门氏菌的快速检测方法[j]。[19]张建军,张建军,张建军,等。PCR-invA基因的克隆及克隆分析。[20]张建军,张建军,张建军。环介导等温扩增(LAMP)[21]赵旭,王磊,褚健,李勇,李勇,徐铮,等。开发和应用一种快速简便的…[22]张勇,单祥,石磊,卢鑫,唐松,王勇,等。基于纤维的环介导等温…[23]王丽,石丽,阿兰敏,耿勇,李丽。环介导等温扩增法对食源性沙门氏菌的特异性快速检测。食品科学,2008;41(1):69-74。[24]陈忠,张凯,尹慧,李强,王玲,刘铮。环介导等温扩增法检测食品中沙门氏菌及几种常见血清型。FSHW。2015; 4(2): 75 - 9。[25]王晓明,王晓明,王晓明,等。环介导等温扩增技术研究进展。背景:沙门氏菌引起的食源性疾病被认为是一个全球性的健康问题,特别是在低收入国家。快速和特异性地检测这种感染源对疫情控制非常重要。本研究旨在设计和优化一种LAMP检测方法,并与PCR法比较其检测食品中伤寒沙门氏菌的灵敏度和效率。材料与方法:将蛋黄酱、蔬菜沙拉等食品样品接种伤寒沙门氏菌。对不同浓度的污染蛋黄酱和蔬菜沙拉进行了LAMP试验的灵敏度和检出限研究。选择invA基因作为目的基因进行PCR和LAMP检测。结果:采用LAMP和PCR方法估计Samonella的检出限为16 CFU/mL。LAMP反应显示明显的浑浊,表明所选靶基因扩增准确,在所研究的食品样品中,不同稀释度下沙门氏菌的正确鉴定。结论:LAMP是一种快速、经济、特异的沙门氏菌鉴别方法。这种方法可以在实验室中使用最少的设备,而不需要昂贵的分子检测方法。
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引用次数: 0
A Review of COVID-19 Survival Potential in Food and Prevention Approaches COVID-19在食品中的生存潜力及预防方法综述
Q4 Medicine Pub Date : 2020-11-01 DOI: 10.29252/IEM.6.4.311
Mahsa Alikord, E. Molaee-aghaee, M. Rostam, Mehri Fallah Raufi
CITATION LINKS A R T I C L E I N F O Article Type Review Article Authors Mahsa Alikord, PhD1* Ebrahim Molaee-aghaee, PhD1* Mohammadreza Rostami, PhD1 Mehri Fallah raufi, MD2 How to cite this article Alikord M., Molaee-aghaee E., Rostami M.R., Fallah raufi M. A Review of COVID-19 Survival Potential in Food and Prevention Approaches. Infection Epidemiology and Microbiology. 2020;6(4): 311-326 1Department of Environmental Health, Food Safety Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. * Correspondence Address: Department of Environmental Health, Food Safety Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. malikord@razi.tums.ac.ir emolaeeaghaee@sina.tums.ac.ir Article History Received: September 05 ,2020 Accepted: November 05 ,2020 Published: November 19 ,2020 Background: The new unknown pandemic introduced in December 2019 in China is now known as SARS-CoV-2 induced COVID-19 (Coronavirus Disease 2019) disease. Some studies have been published by World Health Organization (WHO), Environmental Protection Agency (EPA), and Food and Drug Administration (FDA); however, there is a little information about food safety and COVID-19. The world has not sufficiently addressed the effects of COVID-19 on food safety. The remarkable point is the hypothesis that this epidemic has passed through a food source eaten by an individual and subsequently turned humans into an intermediate host. In particular, the recent state of information about SARS-CoV-2 is challenging owing to its high transmission and mortality rate in people as a potential source of pathogen and infections. However, there is currently no evidence about COVID-19 spread through food. Materials & Methods: Due to the fact that food is a basic humans need and could be an indirect carrier for the virus; therefore, hygiene protocols must be carefully implemented. Also, some studies have suggested that taking supplements, fermented dairy foods, probiotic products, as well as Vitamins C and D could be helpful. Heat treatment and pasteurization could prevent SARS-CoV-2 transmission through food. Conclusion: It is recommended that further studies be performed on the duration of COVs survival at different levels of contact with foods under certain conditions with nanoparticles, nano-packaging, nano-emulsions, and nano-encapsulation to evaluate their size effect. Copyright© 2020, TMU Press. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. 10.29252/iem.6.4.311
A R TI C L E IN F O文章类型综述文章作者Mahsa Alikord, phd * Ebrahim Molaee-aghaee, phd * Mohammadreza Rostami, phd Mehri Fallah raufi, MD2如何引用本文Alikord M., Molaee-aghaee E., Rostami M.R, Fallah raufi M.新冠病毒在食品和预防方法中的生存潜力综述1德黑兰医科大学公共卫生学院环境卫生系,食品安全系,伊朗德黑兰;2德黑兰大学兽医学院,伊朗德黑兰。*通讯地址:伊朗德黑兰德黑兰医科大学公共卫生学院食品安全处环境卫生系。malikord@razi.tums.ac.ir emolaeeaghaee@sina.tums.ac.ir文章历史收稿日期:2020年9月05日收稿日期:2020年11月05日发布日期:2020年11月19日背景:2019年12月在中国引入的新型未知大流行,现称为SARS-CoV-2诱导的COVID-19(冠状病毒病2019)病。世界卫生组织(WHO)、美国环境保护署(EPA)和美国食品和药物管理局(FDA)发表了一些研究报告;然而,关于食品安全和COVID-19的信息很少。全球尚未充分应对COVID-19对食品安全的影响。值得注意的一点是,这种流行病是通过个体食用的食物来源传播的,随后将人类变成了中间宿主。特别是,由于SARS-CoV-2作为病原体和感染的潜在来源,其在人群中的高传播率和死亡率,最近关于SARS-CoV-2的信息状况具有挑战性。然而,目前没有证据表明COVID-19会通过食物传播。材料与方法:由于食物是人类的基本需求,可能是病毒的间接载体;因此,卫生规程必须认真执行。此外,一些研究表明,服用补充剂、发酵乳制品、益生菌产品以及维生素C和D可能会有所帮助。热处理和巴氏消毒可以防止SARS-CoV-2通过食物传播。结论:建议进一步研究纳米颗粒、纳米包装、纳米乳剂和纳米胶囊在一定条件下不同程度与食品接触的冠状病毒的存活时间,以评价其尺寸效应。版权所有©2020,TMU出版社。这篇开放获取的文章是在知识共享署名-非商业4.0国际许可证的条款下发布的,该许可证允许在署名-非商业条款下共享(以任何媒介或格式复制和再分发材料)和改编(重新混合,转换和构建材料)。10.29252 / iem.6.4.311
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引用次数: 3
The Main Causes of Mortality in the Respiratory ICU and Infectious diseases Ward of Sina Hospital in Hamadan 哈马丹市新浪医院呼吸道重症监护病房和传染病病房主要死亡原因分析
Q4 Medicine Pub Date : 2020-06-01 DOI: 10.29252/iem.6.2.135
F. Keramat, Sima Sadat Ahmadinejad, Z. Sanaei, A. Saadatmand
Article Type Original Research Authors Fariba keramat, MD1, 2 Sima Sadat Ahmadinejad, MD3* Zahra Sanaei, PhD4 Ali Saadatmand, MSc1 How to cite this article keramat F., Ahmadinejad S.S., Sanaei Z., Saadatmand A., The Main Causes of Mortality in the Respiratory ICU and Infectious diseases Ward of Sina Hospital in Hamadan. Infection Epidemiology and Microbiology. 2020;6(2): 135-141 1 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. 2 Department of Infectious Diseases, Sina Hospital, Hamadan University of Medical Sciences, Hamadan, Iran. 3 General Practitioner, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. 4 Department of Community Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. * Correspondence Address: Department of Infectious Diseases, Sina Hospital, Hamadan University of Medical Sciences, Hamadan, Iran. simasadatahmadinejad@gmail.com Article History Received: March 15 ,2020 Accepted: May 15 ,2020 Published: June 10 ,2020 [1] Kasper DL, Madoff LC. Introduction to infectious ... [2] Gupta I, Guin P. Communicable diseases in the South-East... [3] Boutayeb A, Boutayeb S. The burden of non communicable diseases in ... [4] Lankarani KB, Alavian SM, Peymani P. Health in the Islamic... [5] Wester AL, Dunlop O, Melby KK, Dahle UR, Wyller TB. Age-related... [6] Fernández de la Hoz K, de Mateo S, Regidor E. Trends in infectious... [7] Dore GJ, Li Y, Plant AJ, Kaldor JM. Trends in infectious disease... [8] TavanaeeSani A, Mirkarimi H. Evaluation of etiology of infectious ... [9] Mouton CP, Bazaldua OV, Pierce B, Espino DV. Common infections in older... [10] Armstrong GL, Conn LA, Pinner RW. Trends in infectious disease mortality in the United... [11] Golsha R, Golshah E, Jalali Z, Rajabli N, Roshandel G. Final diagnosis and outcomes in old patients admitted to the infectious diseases ward. Med J Mashhad Univ... [12] Hasibi M, Soudbakhsh A, Abadi Z, Mehdipoor P. Mortality rate of infectious disease in relation to holidays: Three year study in Imam Khomeini... [13] Mahmoudi GA, Astaraki P, Anbari K, Khayatpisheh S. Epidemiolgical survey of mortality rate in patients admitted to Shohada hospital of Khorramabad in... [14] Noorizad S, Tabesh H, Mahdian M, Akbari H, Taghadosi M. Causes of mortality and morbidity in a neurosurgery ICU in... [15] Norman DC, Grahn D, Yoshikawa TT. Fever and aging. J Am Geriatr... [16] Norman DC, Yoshikawa TT. Fever in the elderly. Infect Dis Clin... [17] Almario CV, Metz DC, Haynes K, Yang YX. Risk of community-acquired pneumonia in patients with a diagnosis of pernicious anemia: A population-based retrospective cohort study. Eur J Gastroenterol ... [18] Serraino D, Bidoli E, Piselli P, Angeletti C, Bruzzone S, Pappagallo M, et al. Time trends in infectious disease mortality in Italy: 1999-1969. Epidemiol... [19] Tellioğlu E, Balcı G, Mertoğlu A. Duration of stay of patients with community-acquired pneumonia in influenza season. Turk
文章类型原创作者Fariba keramat, md1,2 Sima Sadat Ahmadinejad, MD3* Zahra Sanaei, PhD4 Ali Saadatmand, MSc1本文引用方式:keramat F., Ahmadinejad S.S., Sanaei Z., Saadatmand A.,哈马丹市新浪医院呼吸道重症监护病房及感染性疾病病区主要死亡原因分析。感染流行病学与微生物学,2020;6(2):135-141 1伊朗哈马丹医学院布鲁氏菌病研究中心,伊朗哈马丹。2伊朗哈马丹医科大学Sina医院感染性疾病科。3伊朗哈马丹医科大学医学院全科医生,伊朗哈马丹。4伊朗哈马丹医科大学医学院社区医学系,伊朗哈马丹。*通讯地址:伊朗哈马丹医学院Sina医院传染病科。simasadatahmadinejad@gmail.com文章历史收稿日期:2020年3月15日收稿日期:2020年5月15日发布日期:2020年6月10日[1]Kasper DL, Madoff LC。介绍传染性…[2]古普塔,金。东南地区传染病…[3]张建军,张建军,张建军,等。北京市非传染性疾病负担的研究进展[4]刘建军,刘建军,刘建军,等。[5]刘建军,刘建军,李建军,等。与年龄相关的…[6]张建军,张建军,张建军,等。中国传染病流行病学研究进展[j] . Fernández[7]张建军,李勇。传染病趋势…[8]王晓明,王晓明,王晓明,等。猪传染性疾病的病原学分析。[9]张建军,李建军,张建军。老年人常见的感染…[10]王志强,王志强,王志强。美国传染病死亡率趋势[11]王晓明,王晓明,王晓明,等。老年感染性疾病患者的临床诊断及预后分析。马什哈德医学院…[12]王晓明,王晓明,王晓明,等。中国农村人口传染性疾病的研究进展[j]。[13]张建军,张建军,张建军,等。河南省医院住院病人死亡率调查[j]。[14]王晓华,王晓华,王晓华,等。急性脑卒中重症监护病房的临床分析[j]。[15]李建军,李建军,李建军。发烧和衰老。我是杰里亚特……[16]李志强,李志强。老年人发烧。感染与疾病临床…[17]杨永平,杨永平,杨永平。诊断为恶性贫血的患者发生社区获得性肺炎的风险:一项基于人群的回顾性队列研究Eur J Gastroenterol…[18]张晓明,张晓明,张晓明,等。意大利传染病死亡率的时间趋势:1999-1969年。论文……[19] Tellioğlu E, balcki, Mertoğlu A.流感季节社区获得性肺炎患者住院时间分析。Turk Thorac……[20]王晓明,王晓明,王晓明,等。肺炎住院时间的缩短和出院后的结果。我…目的:在世界许多地方,传染病是可能导致死亡的最严重疾病之一。本研究旨在调查哈马丹市新浪医院感染病区及呼吸内科ICU住院患者的主要死亡原因。资料与方法:对2012年3月至2017年3月感染病区及呼吸内科ICU住院患者的病历进行调查,对住院期间死亡的患者进行统计。结果:共回顾593例患者的病历,其中534例纳入本研究。其中呼吸道ICU住院患者426例(79.8%),感染病房住院患者108例(20.2%),2012 - 2016年死亡率计算为:2012年4.69%,2013年6.31%,2014年4.68%,2015年5.2%,2016年5.8%。本研究结果显示,呼吸道ICU最常见的死亡原因是败血症(44.4%),感染病房最常见的死亡原因是肺炎(25.3%)。病死率最高的是败血症(237例,48.2%)和肺栓塞(15例,44.1%)。结论:本研究结果显示,脓毒症和肺炎是感染性疾病病房和呼吸内科ICU住院患者最常见的死亡原因;此外,败血症和肺栓塞的致死率最高。
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引用次数: 0
Prevalence of blaTEM and blaVEB Genes in Multidrug-Resistant Acinetobacter baumannii Strains Isolated from Burn Wound Infection in Isfahan, Iran 伊朗伊斯法罕烧伤感染多药耐药鲍曼不动杆菌株中blaTEM和blaVEB基因的流行
Q4 Medicine Pub Date : 2020-06-01 DOI: 10.29252/iem.6.2.109
Elham Haghighifar, R. Dolatabadi, A. Rezaei
Article History Received: March 15 ,2020 Accepted: May 15 ,2020 Published: June 10 ,2020 [1] Hashim IA, Wdaah QH, Atya AA. Potential effect of ... [2] Peck MD. Epidemiology of burns throughout the world. Part I: Distribution and ... [3] Capoor MR., Sarabahi S, Tiwari VK, Narayanan RP. Fungal ... [4] Zubair M, Malik A, Ahmad J, Rizvi M, Farooqui KJ, Rizvi MW. A study of ... [5] Asadollahi P, Akbari M, Soroush S, Taherikalani M, Asadollahi K, Sayehmiri K, et al. Antimicrobial resistance patterns and their encoding genes ... [6] Rezaei A, Fazeli H, Moghadampour M, Halaji M, Faghri J. Determination of antibiotic resistance pattern and prevalence of OXA-type carbapenemases among Acinetobacter baumannii clinical isolates from inpatients in Isfahan, central ... [7] Rezaei A, Fazeli H, Halaji M, Moghadampour M, Faghri J. Prevalence of ... [8] Heidari H, Halaji M, Taji A, Kazemian H, Abadi MSS, Sisakht MT, et al. Molecular analysis of drug-resistant ... [9] García-Patiño MG, García-Contreras R, Licona-Limón P. The immune response against Acinetobacter baumannii, an ... [10] Kittinger C, Kirschner A, Lipp M, Baumert R, Mascher F, Farnleitner AH, et al. Antibiotic resistance of Acinetobacter spp. isolates from the river ... [11] JafariSales A, Bagherizadeh Y, Khalifehpour M, Abdoli-senejan M, Helali-Pargali R. Antibiotic resistance pattern and blaTEM gene expression in Acinetobacter baumannii isolated from clinical specimens ... [12] Ghaima KK, Abdulhassan AA, Mahdi ZH. Molecular study of ... [13] Torkar KG, Bedenić B. Antimicrobial susceptibility and characterization of metalloβ-lactamases, extended ... [14] Fazeli H, Taraghian A, Poursina F, Kamali R. Molecular ... [15] Sohail M, Rashid A, Aslam B, Waseem M, Shahid M, Akram M, et al. Antimicrobial ... [16] Rezai MS, Rafiei A, Ahangarkani F, Bagheri-Nesami M, Nikkhah A, Shafahi K, et al. Emergence ... [17] Burcu B, Acik L, Sultan L. Phenotypic and molecular ... [18] Vahidi Emami H, Khalilian M, Yadollahi Movahhed N. Antibiotic ... [19] Moosavian M, Sirous M, Shams N. Phenotypic and ... [20] Bali EB, Accedil L, Sultan N. Phenotypic ... [21] Salih TS, Shafeek RR. In silico detection ... [22] Abdar MH, Taheri-Kalani M, Taheri K, Emadi B, Hasanzadeh A, Sedighi A, et al. Prevalence of ... [23] Ahmadikiya F, Mosadegh A, Moradi M, Hossieni-Nave H. Antimicrobial ... [24] Azizi O, Shahcheraghi F. The frequency of blaPER, blaVEB, blaCTX-M, tetA and tetB genes among ... [25] Shahcheraghi F, Akbari Shahmirzadi N, Abbasalipour Bashash M, Jabbar H, Amirmozafari N. Detection ... [26] Safari M, Nejad ASM, Bahador A, Jafari R, Alikhani MY. Prevalence of ... Aims: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii strains. The aim of this study was to determine the frequency of blaTEM and blaVEB genes in clinical isolates of A. baumannii and the relationship between the anti
收稿日期:2020年3月15日收稿日期:2020年5月15日发布日期:2020年6月10日[1]哈希姆·IA, Wdaah QH, Atya AA。的潜在影响……[2]李建军,李建军。烧伤流行病学研究进展。第一部分:分布和……[3]刘建军,刘建军,李建军。真菌……[4]李建军,李建军,李建军,等。对……的研究[5]李建军,李建军,李建军,等。抗菌素耐药性模式及其编码基因…[6]张晓明,王晓明,王晓明,等。云南省云南省不同类型鲍曼不动杆菌的耐药特征及耐药性分析[j]。[7]李建军,李建军,李建军,等。中国烟草烟草行业的发展趋势。[8]王晓明,王晓明,王晓明,等。耐药分子分析…[9] García-Patiño王志强,García-Contreras R, Licona-Limón P.鲍曼不动杆菌的免疫应答,[j]。[10]李建军,李建军,李建军,等。河流中不动杆菌的耐药性研究…[11]张建军,张建军,张建军,等。鲍曼不动杆菌blaTEM基因的克隆及耐药性分析[j]。[12]张建军,张建军,张建军。的分子研究……[13]张建军,张建军,张建军,等。金属β-内酰胺酶的研究进展[j]。[14]王晓明,王晓明,王晓明,等。[15]李建军,李建军,李建军,等。抗菌……[16]李建军,李建军,李建军,等。出现……[17]李建军,李建军,李建军,等。[18]张晓明,张晓明,张晓明,等。抗菌药物的研究进展。[19]张建军,张建军,张建军,等。[20]李建军,李建军,李建军,等。[21]张志强,李志强。在硅检测…[22]李建军,李建军,李建军,等。流行的……[23]王晓明,王晓明,王晓明,等。[24]王晓明,王晓明,王晓明,等。中国小麦blaPER、blaVEB、blaCTX-M、tetA和tetB基因多态性分析。[25]张建军,张建军,张建军,等。[26]张建军,张建军,张建军,等。流行的……目的:鲍曼不动杆菌是一种条件致病菌,对包括β -内酰胺在内的许多抗生素具有耐药性。β-内酰胺酶的产生是鲍曼不动杆菌产生β-内酰胺耐药的主要机制。本研究的目的是确定临床分离的鲍曼不动杆菌中blabem和blaVEB基因的频率,以及在伊斯法罕烧伤创面感染分离的菌株中ESBL基因的存在与抗生素耐药性的关系。材料与方法:本研究从烧伤创面感染中分离出123株耐多药鲍曼不动杆菌。采用Kirby-Bauer圆盘扩散法进行抗生素耐药性评估后,采用聚合酶链反应(PCR)技术检测ESBL基因,最后进行统计学分析。结果:123株鲍曼不动杆菌PCR结果显示,77株(62.60%)为ESBL阳性。blaTEM和blaVEB基因的频率分别为52个(42.3%)和67个(54.5%)。鲍曼不动杆菌对抗生素的耐药性与ESBL基因(blaTEM和blaVEB)的存在有显著关系。结论:本研究发现的鲍曼不动杆菌株中blabem和blaVEB基因的高流行率是伊斯法罕和伊朗地区烧伤创面感染的主要问题,因为这些菌株引起的感染治疗非常复杂。本研究结果强调需要采取感染控制措施,以防止耐药分离株和ESBL基因的传播,特别是在烧伤医院。
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引用次数: 0
Measuring the Level of Main Mycotoxins in Wheat Samples Collected from Flour Factories Silos in Alborz and Tehran Provinces Using LC-MS/MS 用LC-MS/MS法测定Alborz省和德黑兰省面粉厂筒仓小麦样品中主要真菌毒素的含量
Q4 Medicine Pub Date : 2020-06-01 DOI: 10.29252/iem.6.2.95
Ghasem Heidari, S. J. H. Hazaveh, M. Bayat, B. Daraei
Article Type Original Research Authors Ghasem Heidari, PhD Seyed Jamal Hashemi Hazaveh, PhD1 Mansour Bayat, PhD1, Bahram Daraei, PhD3* How to cite this article Heidari GH., Hashemi Hazaveh S. J., Bayat M., Daraei B. Measuring the Level of Main Mycotoxins in Wheat Samples Collected from Flour Factories Silos in Alborz and Tehran Provinces Using LC-MS/ MS. Infection Epidemiology and Microbiology. 2020;6(2): 95-107 1 Department of pathobiology, Science and Research Branch, Islamic Azad University,Tehran,Iran 2 Department of Medical Parasitology and Mycology, School of Public Health, Food Microbiology Research Center, Tehran University of Medical Sciences,Tehran,Iran 3 Departement of Toxicology and Pharmacology, School of pharmacy, Shahid Beheshti University of Medical Sciences,Tehran,Iran * Correspondence Address: Departement of Toxicology and Pharmacology, School of pharmacy, Shahid Beheshti University of Medical Sciences,Tehran,Iran. bdaraei@sbmu.ac.ir Article History Received: April 05 ,2020 Accepted: March 30 ,2020 Published: June 10 ,2020 [1] Bhat R, Rai RV, Karim AA. Mycotoxins ... [2] Cheng CT, Perak M. Mass poisoning tale of ... [3] Bryden WL. Mycotoxin contamination of the feed supply chain: Implications for animal productivity and ... [4] Marquardt RR. Effects of molds and their toxins on livestock performance: A western Canadian perspective. Anim Feed ... [5] Richard JL. Some major mycotoxins and their mycotoxicosesAn overview. Int J Food ... [6] Kim DH, Hong SY, Kang JW, Cho SM, Lee KR, An TK, et al. Simultaneous determination of ... [7] Vaclavikova M, MacMahon S, Zhang K, Begley TH. Application of single immunoaffinity clean-up for ... [8] Park J, Kim DH, Moon JY, An JA, Kim YW, Chung SH, et al. Distribution analysis of ... [9] Sarrafian MR. Country report: Iran. Thailand, Chiang Rai: Fourth session of the technical ... [10] Reddy CS. Mycotoxin ... [11] Zamani-Zadeh HR, Khoursandi H. Occurrence of ... [12] Sulyok M, Berthiller F, Krska R, Schuhmacher R. Development and... [13] Rasmussen RR, Storm IMLD, Rasmussen PH, Smedsgaard J, Nielsen K F. Multi-mycotoxin analysis of ... [14] Sorensen LK, Elbaek TH. Determination... [15] Frenich AG, Vidal JLM, Romero-Gonzalez R, Aguilera-Luiz MM. Simple and ... [16] Razzazi-Fazeli E. A review of recent trends in applications of liquid chromatography-mass spectrometry for determination of mycotoxins. J Liq Chromatogr ... [17] Di Mavungu JD, Monbaliu S, Scippo ML, Maghuin Rogister G, Schneider YJ, Larondelle Y, et al. LC-MS/MS multi-analyte method for ... [18] Monbaliu S, Van Poucke C, van Peteghem C, Van Poucke K, Heungens K, de Saeger S. Development of ... [19] Boyd RK, Basic C, Bethem RA. Trace ... [20] Ediage EN, Di Mavungu JD, Monbaliu S, Van Peteghem C, De Saeger S. A validated multianalyte LC–MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and ... [21] Ediage EN, Van Poucke C, De Saeger S. A multi-analyte... [22] Commission Regulation ... [23] Soleimany F, Jinap S,
文章类型原始研究作者Ghasem Heidari, Seyed Jamal Hashemi Hazaveh, PhD1 Mansour Bayat, PhD1, Bahram Daraei, PhD3*如何引用本文Heidari GH。[3]张建军,张建军,张建军,等。基于LC-MS/ ms法测定小麦主要真菌毒素含量的研究进展[J] .中国农业科学,2010;6(2):95-107 1伊朗德黑兰伊斯兰阿扎德大学科学与研究分部病理生物学教研室2伊朗德黑兰德黑兰医科大学食品微生物研究中心公共卫生学院医学寄生虫学与真菌学教研室3伊朗德黑兰Shahid Beheshti医科大学药学院毒理学与药学系*通讯地址:伊朗德黑兰Shahid Beheshti医科大学药学院毒理学与药理学教研室。bdaraei@sbmu.ac.ir文章历史收稿日期:2020年04月05日收稿日期:2020年03月30日发布日期:2020年06月10日[1]Bhat R, Rai RV, Karim AA。真菌毒素……[2] Cheng CT, Perak M.大规模中毒事件…b[3]布莱顿WL。霉菌毒素污染的饲料供应链:对动物生产力的影响和…b[4]马夸特RR。霉菌及其毒素对牲畜生产性能的影响:加拿大西部的观点。动物饲料…b[5] Richard JL。一些主要的真菌毒素及其真菌毒性综述。Int J Food…[10]金东辉,洪世文,姜建文,赵思敏,李克仁,安国坤,等。同时测定……[10]张建军,张建军,张建军。单免疫亲和清理技术在…[10]朴俊,金德华,文建勇,安佳,金耀文,钟山,等。的分布分析。国家报道:伊朗。泰国,清莱:第四届技术…Reddy CS。霉菌毒素……[11] Zamani-Zadeh, Khoursandi H.…[12] Sulyok M, Berthiller F, Krska R, Schuhmacher R.发展与…[10]杨建军,李建军,李建军,等。真菌毒素分析方法的研究进展。[14] Sorensen LK, Elbaek TH。决心……[15] Frenich AG, Vidal JLM, Romero-Gonzalez R, Aguilera-Luiz MM。[10]刘志强,刘志强。液相色谱-质谱法检测真菌毒素的研究进展。[J]液相色谱…[10] Di Mavungu JD, Monbaliu S, Scippo ML, Maghuin roister G, Schneider YJ, Larondelle Y,等。LC-MS/MS多分析方法测定…[10]孟巴鲁,Van Poucke, Van Peteghem, Van Poucke K, Heungens K, de Saeger S。[19] Boyd RK, Basic C, Bethem RA。跟踪……[10]王晓明,王晓明,王晓明,等。hplc - MS/MS法测定木薯粉、花生蛋糕和玉米中25种真菌毒素的含量[10]王志强,王志强,王志强,等。[22]委员会条例……[10]李建军,李建军,李建军,等。测定……[24] Kumar R, Ansari KM, Saxena N, Dwivedi PD, Jain SK, Das M.检测…[10]周强,李峰,陈磊,姜东。[26] Hussein HS, Brasel JM。毒性,新陈代谢,还有…[27] Yazdanpanah H, Miraglia M, Calfapietra FR, Brera C. Natural…[10] Zinedine A, Brera C, Elakhdari S, Catano C, Debegnach F, Angelinib S,等. ...目的:谷物和谷物制品容易被产生真菌毒素的真菌感染。本研究旨在利用UHPLC-MS/MS装置对德黑兰和阿尔博尔兹省小麦筒仓样品中11种主要真菌毒素的污染水平进行调查。材料与方法:样品制备分别采用乙腈/水/乙酸溶剂和Myco6in1免疫亲和柱进行提取和纯化。采用反相超高效液相色谱-串联质谱(UHPLC-MS/MS)和电喷雾电离技术,在正离子模式下,在MRM程序中运行15分钟,同时检测选定的真菌毒素。采用标度曲线法克服基质效应,测定霉菌毒素残留量。结果:AFB1和OTA真菌毒素的定量限和检出限分别为2和0.7 ppb;DON, FB1和FB2分别为100和33.3 ppb;ZER分别为50和16.6 ppb; AFB2、AFG1、AFG2和T-2分别为5和1.6 ppb;HT-2分别为20和6.6 ppb。该方法检测真菌毒素的准确度和线性良好。霉菌毒素的平均回收率为72 ~ 123%,相对标准偏差(RSDr)为0.6 ~ 24.2%,说明方法的准确性。采用验证的方法对30份小麦样品进行真菌毒素残留评价。在小麦样品中发现了OTA、T-2和HT-2真菌毒素。
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Infection Epidemiology and Microbiology
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