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Association of Anti-N and -S Seroprevalence in Asymptom atic, Mildly Symptomatic, and Symptomatic SARS-COV-2 Natural Infection 无症状、轻度症状和有症状的SARS-COV-2自然感染中抗n和-S血清阳性率的相关性
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.357
N. Hajiahmadi, Faezeh Mojtahedzade, A. Yari, M. Tat, H. Soleimanjahi, saeed amel jamehdar, M. Jafari, Samira Asli, R. Dorostkar, M. Nazemipour, M. Mansournia, T. Bamdad
Backgrounds: SARS-COV-2 infection is not always correlated with protection. Antibody seroprevalence in unvaccinated individuals, which is usually measured by N-specific antibodies, is not necessarily correlated with protection, while antibodies against S protein show a better correlation with protection due to its neutralizing epitopes. In this study, we tried to improve our conception of the hidden perspective of SARS-COV-2 in epidemiological reports and investigate anti-S antibody prevalence among anti-N antibody-positive asymptomatic and mildly symptomatic patients. Material(s) and Method(s): Blood samples were collected from asymptomatic or mildly symptomatic volunteer participants and symptomatic hospitalized patients with negative PCR results from May 30 to June 17, 2020. Detection of SARS-COV-2 antibodies was done using an ELISA kit targeting N or S protein. Finding(s): Totally, 716 samples from volunteer participants and 81 samples from symptomatic hospitalized patients with negative PCR results were evaluated. The test performance-adjusted seroprevalence (%95 CI) of SARS-COV-2 antibody was 17.3% (8.8-25.8%) for anti-N IgG in volunteers and 25.5% (12.8-39.7%) for anti-N and anti-S IgM in hospitalized patients. Among anti-N IgG positive infected individuals, %49.2 (21.4 and 78.8%) were anti-S antibody positive. Conclusion(s): The results showed that SARS-COV-2 infection sometimes occurs in individuals without symptoms or with mild symptoms, but in more than half of them, the produced antibody is not protective. The findings of hospitalized patients showed that the combination of IgM assay with real-time PCR improved the disease diagnosis by more than 25% in cases with negative molecular test results.Copyright © 2022, TMU Press.
背景:SARS-COV-2感染并不总是与保护相关。在未接种疫苗的个体中,通常用n特异性抗体测量的抗体血清阳性率与保护作用不一定相关,而针对S蛋白的抗体由于其中和表位而与保护作用表现出更好的相关性。在本研究中,我们试图完善流行病学报告中SARS-COV-2隐藏视角的概念,并调查抗n抗体阳性无症状和轻度症状患者的抗s抗体患病率。材料和方法:于2020年5月30日至6月17日采集无症状或轻度症状志愿者和PCR阴性的有症状住院患者的血液样本。采用针对N或S蛋白的ELISA试剂盒检测SARS-COV-2抗体。结果:共评估了志愿者716份样本和PCR阴性的有症状住院患者81份样本。志愿者抗n IgG血清阳性率(%95 CI)为17.3%(8.8-25.8%),住院患者抗n和抗s IgM血清阳性率(%95 CI)为25.5%(12.8-39.7%)。抗n IgG阳性感染者中,抗s抗体阳性的占比分别为21.4%和78.8%。结论:结果表明,SARS-COV-2感染有时发生在无症状或症状轻微的个体中,但在半数以上的个体中,产生的抗体不具有保护作用。住院患者的研究结果显示,在分子检测结果阴性的病例中,IgM检测与实时荧光定量PCR相结合可使疾病诊断提高25%以上。版权所有©2022,TMU出版社。
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引用次数: 0
High Incidence of MDR and XDR Pseudomonas aeruginosa Isolates Harboring blaGES, blaVEB, and blaPER Extended-Spectrum Beta-Lactamase Genes in Iran 伊朗耐多药和广泛耐药铜绿假单胞菌携带blaGES、blaVEB和blaPER扩展谱β -内酰胺酶基因的高发
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.285
Fahimeh Beiki, A. Tavakoli Kareshk, P. Askari, A. Hashemi, M. Yousefi
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引用次数: 0
Evaluation of the Synergistic Interaction between Satureja hortensis and Carum carvi Essential Oils and Fluconazole against Candida albicans: In Vitro 红花香精油与氟康唑对白色念珠菌体外协同作用的评价
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.327
Yasaman karbalaei, S. Amanloo, N. Gholami, A. Yazdinezhad
{"title":"Evaluation of the Synergistic Interaction between Satureja hortensis and Carum carvi Essential Oils and Fluconazole against Candida albicans: In Vitro","authors":"Yasaman karbalaei, S. Amanloo, N. Gholami, A. Yazdinezhad","doi":"10.52547/iem.8.4.327","DOIUrl":"https://doi.org/10.52547/iem.8.4.327","url":null,"abstract":"","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84955676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arboviruses, an Emerging Threat to Public Health: Focus on Nigeria, West Africa 虫媒病毒,对公共卫生的新威胁:重点关注西非尼日利亚
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.379
R. Adesola, I. Idris
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引用次数: 0
Molecular Detection and Characterization of Herpesvirus Papio 2 (HVP2) in Wild-Caught Olive Baboons from Selected Regions in Kenya 肯尼亚部分地区野生橄榄狒狒中疱疹病毒2型(HVP2)的分子检测和鉴定
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.345
Gloria Kemunto, Mbogo Kevin, Kamau W. Joseph, A. Nyachieo
{"title":"Molecular Detection and Characterization of Herpesvirus Papio 2 (HVP2) in Wild-Caught Olive Baboons from Selected Regions in Kenya","authors":"Gloria Kemunto, Mbogo Kevin, Kamau W. Joseph, A. Nyachieo","doi":"10.52547/iem.8.4.345","DOIUrl":"https://doi.org/10.52547/iem.8.4.345","url":null,"abstract":"","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85890518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Detection of Blastocystis Subtypes in Domestic Pigeons and Their Owners in Tafresh City 塔什市家鸽及其饲主囊虫亚型的分子检测
Q4 Medicine Pub Date : 2022-12-01 DOI: 10.52547/iem.8.4.317
Samane Abdolahi, A. Dalimi, Majid Piranestani
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引用次数: 0
Phenotypic and Genotypic Characterization of Antibiotic Resistance in Escherichia coli Strains Isolated from Broiler Chickens with Suspected Colibacillosis in Isfahan Province, Iran 伊朗伊斯法罕省疑似大肠杆菌病肉鸡大肠杆菌耐药表型和基因型分析
Q4 Medicine Pub Date : 2022-09-01 DOI: 10.52547/iem.8.3.193
M. Horri, M. Gholami-Ahangaran
the qnrA and sul1 genes were amplified with specific primers. Findings: The results showed that 85% of E. coli isolates were resistant to at least two antibiotics, and 6% of E. coli strains were resistant to all 13 antibiotics used in this study. E. coli isolates showed the highest resistance to enrofloxacin (70%) and the lowest resistance to gentamicin (6%). Examination of resistance genes showed that about 54% of enrofloxacin-resistant E. coli strains contained the qnrA gene, and 48% of sulfonamide-resistant E. coli strains contained the sul1 gene. Conclusion: In this study, some resistant strains lacked the resistance genes studied, indicating the importance of other resistance genes in inducing resistance against sulfonamides and fluoroquinolones. Also, the lack of resistance in some strains harboring qnrA and sul1 genes indicates the importance of gene expression in mediating resistance, and that the presence of resistance genes alone is not sufficient to induce antibiotic resistance in E. coli strains.
用特异性引物扩增qnrA和sul1基因。结果显示,85%的大肠杆菌分离株对至少两种抗生素耐药,6%的大肠杆菌菌株对本研究中使用的所有13种抗生素都耐药。大肠杆菌分离株对恩诺沙星的耐药性最高(70%),对庆大霉素的耐药性最低(6%)。耐药基因检测显示,54%的恩诺沙星耐药大肠杆菌含有qnrA基因,48%的磺胺耐药大肠杆菌含有sul1基因。结论:本研究中部分耐药菌株缺乏所研究的耐药基因,提示其他耐药基因在诱导对磺胺类药物和氟喹诺酮类药物耐药中的重要性。此外,一些含有qnrA和sul1基因的菌株缺乏耐药,表明基因表达在介导耐药中的重要性,仅存在耐药基因不足以诱导大肠杆菌菌株对抗生素产生耐药。
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引用次数: 0
Prevalence of ESBLs and Biofilm Formation in Escherichia coli Isolated from Urinary Tract Infection in Isfahan, Iran 伊朗伊斯法罕地区尿路感染分离大肠埃希菌ESBLs流行及生物膜形成
Q4 Medicine Pub Date : 2022-09-01 DOI: 10.52547/iem.8.3.223
Elham Haghighifar, A. Rezaei
was detected in 93 (66.9%) isolates. The PCR test results showed that
检出93株(66.9%)。PCR检测结果显示
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引用次数: 0
Assessment of Antifungal Activity of Saccharomyces boulardii against Candida albicans Biofilm 博氏酵母菌对白色念珠菌生物膜的抑菌活性评价
Q4 Medicine Pub Date : 2022-09-01 DOI: 10.52547/iem.8.3.243
Z. Sadeghi Ardestani, M. Yadegari, R. Kachuei
) and MTT reduction reaction. The collected data were analyzed using student’s t-test in SPSS statistical software. Findings: In this study, the probiotic yeast S. boulardii reduced the pathogenicity and virulence of C. albicans in vitro. According to the results of CVS and MTT assays, a considerable reduction ( p < .001) in the biomass and viability of C. albicans biofilms was observed after 48 hours of incubation in the presence of S. boulardii extract. Conclusion: There was a significant association between S. boulardii extract concentration and biofilm formation in both CVS and MTT assays. Biofilm formation decreased with increasing S. boulardii extract concentration and incubation time in both methods compared to the control group .
)和MTT还原反应。收集的数据在SPSS统计软件中使用学生t检验进行分析。结果:在本研究中,益生菌酵母博氏梭菌在体外降低了白色念珠菌的致病性和毒力。根据CVS和MTT试验的结果,在博氏弧菌提取物存在下孵育48小时后,观察到白色念珠菌生物膜的生物量和活力显著降低(p < 0.001)。结论:在CVS和MTT试验中,博氏弧菌提取物浓度与生物膜形成有显著相关性。与对照组相比,两种方法的生物膜形成均随博氏弧菌提取物浓度和孵育时间的增加而减少。
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引用次数: 0
Strategies for Vaccine Design for Corona Virus for All Variants of Concern Using Immunoinformatics Techniques 利用免疫信息学技术设计冠状病毒所有变体的疫苗策略
Q4 Medicine Pub Date : 2022-09-01 DOI: 10.52547/iem8.3.259
Anamika Basu
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引用次数: 1
期刊
Infection Epidemiology and Microbiology
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