50 and 21 isolates were identified as E. faecalis and E. faecium, respectively . Most of the Enterococcus species were isolated from urine, followed by wound samples. The most prevalent virulence genes among E. faecalis isolates were cylA (60%) and gelE (30%); also, 19 and 14% of E. faecium isolates were positive for cylA and gelE genes, respectively. Many isolates of E. faecalis (84%) and E. faecium (76%) were resistant to one or more antibiotics and showed high resistance to gentamicin and ciprofloxacin. Conclusion: This study revealed a high prevalence of ciprofloxacin and gentamicin resistance and a high frequency of virulence genes among E. faecalis isolates. Due to the high prevalence of MDR Enterococcus strains, control measures are necessary to prevent the emergence and transmission of these strains in different hospital wards.
{"title":"Virulence Genes and Antibiotic Susceptibility of Enterococcus spp. in Bandar Abbas City, Iran","authors":"T. Dehghani, A. Karmostaji, H. Alizade","doi":"10.52547/iem.8.2.129","DOIUrl":"https://doi.org/10.52547/iem.8.2.129","url":null,"abstract":"50 and 21 isolates were identified as E. faecalis and E. faecium, respectively . Most of the Enterococcus species were isolated from urine, followed by wound samples. The most prevalent virulence genes among E. faecalis isolates were cylA (60%) and gelE (30%); also, 19 and 14% of E. faecium isolates were positive for cylA and gelE genes, respectively. Many isolates of E. faecalis (84%) and E. faecium (76%) were resistant to one or more antibiotics and showed high resistance to gentamicin and ciprofloxacin. Conclusion: This study revealed a high prevalence of ciprofloxacin and gentamicin resistance and a high frequency of virulence genes among E. faecalis isolates. Due to the high prevalence of MDR Enterococcus strains, control measures are necessary to prevent the emergence and transmission of these strains in different hospital wards.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79608322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Setareh Zamani, M. Fazilati, Manijeh Hadian, H. Nazem, N. Noohi
Green synthesis of nanoparticles (NPs) is a simple, fast, and eco-friendly method which could be performed by various microorganisms or plant extracts. Silver NPs are well-known as antimicrobial and anti-fungal materials. They play an essential role in the control of tumors via their cytotoxic effects. Therefore, they have attracted significant attention for developing an effective treatment solution for cancer cells. This study aimed to investigate the potential of Penicillium chrysogenum for the synthesis of silver NPs and to evaluate their toxicity on liver cancer cell line (HepG2). , characterization of the synthesized NPs was performed by UV–Vis spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was carried out to detect biomolecules that may be responsible for the synthesis and stabilization of NPs. The cytotoxic activity of the synthesized AgclNPs on HepG2 cell line was evaluated using MTT assay. Findings: UV–Vis spectroscopy and XRD analysis confirmed the synthesis of AgclNPs using P. chrysogenum . TEM analysis revealed the spherical shape of AgclNPs with an average crystalline size of 15 to 45 nm. FTIR spectroscopy indicated the possible functional groups that could be responsible for the reduction of metal ions and the capping process. These nanoparticles showed a dose-dependent anticancer activity against HepG2 cells. Conclusion: The results suggest that biosynthesized silver chloride nanoparticles could offer potential applications in cancer therapy.
{"title":"Evaluation of Anticancer Potential of Silver Chloride Nanoparticles\u0000Biosynthesized by Penicillium chrysogenum","authors":"Setareh Zamani, M. Fazilati, Manijeh Hadian, H. Nazem, N. Noohi","doi":"10.52547/iem.8.2.159","DOIUrl":"https://doi.org/10.52547/iem.8.2.159","url":null,"abstract":"Green synthesis of nanoparticles (NPs) is a simple, fast, and eco-friendly method which could be performed by various microorganisms or plant extracts. Silver NPs are well-known as antimicrobial and anti-fungal materials. They play an essential role in the control of tumors via their cytotoxic effects. Therefore, they have attracted significant attention for developing an effective treatment solution for cancer cells. This study aimed to investigate the potential of Penicillium chrysogenum for the synthesis of silver NPs and to evaluate their toxicity on liver cancer cell line (HepG2). , characterization of the synthesized NPs was performed by UV–Vis spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was carried out to detect biomolecules that may be responsible for the synthesis and stabilization of NPs. The cytotoxic activity of the synthesized AgclNPs on HepG2 cell line was evaluated using MTT assay. Findings: UV–Vis spectroscopy and XRD analysis confirmed the synthesis of AgclNPs using P. chrysogenum . TEM analysis revealed the spherical shape of AgclNPs with an average crystalline size of 15 to 45 nm. FTIR spectroscopy indicated the possible functional groups that could be responsible for the reduction of metal ions and the capping process. These nanoparticles showed a dose-dependent anticancer activity against HepG2 cells. Conclusion: The results suggest that biosynthesized silver chloride nanoparticles could offer potential applications in cancer therapy.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84800250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Rahsepar, Shahla Roudbar mohammadi, H. Delavari, M. Roudbari, Zuhair Mohammad Hassan
Design and Syn- thesis of Novel Thymoquinone-Ze-in Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm in Vitro. Infection Epidemiology and Microbiology. 2022;8(2): ingredient in Nigella sativa, which has considerable antifungal properties. The aim of this study was to investigate the inhibitory effects of thymoquinone-zein nanoparticles (TQ-ZNPs) on In the current study, TQ was encapsulated in zein (as a biodegradable carrier) and polyethylene glycol (PEG). The antifungal activity of TQ-ZNPs against (ATCC 10231; standard strain) and their inhibitory effects on biofilm formation were examined using standardized broth microdilution and MTT assays, respectively. The total oxidant status (TOS) of C. albicans was assessed using colorimetric method, and the toxic effect of nanoparticles on peripheral blood mononuclear cells (PBMCs) was evaluated by MTT assay. The minimum inhibitory concentration (MIC) of TQ-ZNPs was significantly reduced compared to that of free TQ. MIC values of TQ-ZNPs and free TQ were determined to be 7.4 and 50 µg/mL , respectively. Biofilm formation was inhibited, and oxidant production by fungal cells was increased. The findings of this study showed that TQ-ZNPs had no toxic effect on PBMCs. Conclusion: This study results revealed that the synthesized nanoparticles had a good antifungal activity without any toxicity. The results demonstrated the superior efficiency of TQ-ZNPs over free TQ. Hence, this structure could be used to load hydrophobic drugs. However, more studies are needed to evaluate the beneficial properties of TQ-ZNPs. and fluconazole (0.5 MIC, 1 MIC, and 2 MIC)
{"title":"Design and Synthesis of Novel Thymoquinone-Zein Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm Formation in Vitro","authors":"S. Rahsepar, Shahla Roudbar mohammadi, H. Delavari, M. Roudbari, Zuhair Mohammad Hassan","doi":"10.52547/iem.8.2.169","DOIUrl":"https://doi.org/10.52547/iem.8.2.169","url":null,"abstract":"Design and Syn- thesis of Novel Thymoquinone-Ze-in Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm in Vitro. Infection Epidemiology and Microbiology. 2022;8(2): ingredient in Nigella sativa, which has considerable antifungal properties. The aim of this study was to investigate the inhibitory effects of thymoquinone-zein nanoparticles (TQ-ZNPs) on In the current study, TQ was encapsulated in zein (as a biodegradable carrier) and polyethylene glycol (PEG). The antifungal activity of TQ-ZNPs against (ATCC 10231; standard strain) and their inhibitory effects on biofilm formation were examined using standardized broth microdilution and MTT assays, respectively. The total oxidant status (TOS) of C. albicans was assessed using colorimetric method, and the toxic effect of nanoparticles on peripheral blood mononuclear cells (PBMCs) was evaluated by MTT assay. The minimum inhibitory concentration (MIC) of TQ-ZNPs was significantly reduced compared to that of free TQ. MIC values of TQ-ZNPs and free TQ were determined to be 7.4 and 50 µg/mL , respectively. Biofilm formation was inhibited, and oxidant production by fungal cells was increased. The findings of this study showed that TQ-ZNPs had no toxic effect on PBMCs. Conclusion: This study results revealed that the synthesized nanoparticles had a good antifungal activity without any toxicity. The results demonstrated the superior efficiency of TQ-ZNPs over free TQ. Hence, this structure could be used to load hydrophobic drugs. However, more studies are needed to evaluate the beneficial properties of TQ-ZNPs. and fluconazole (0.5 MIC, 1 MIC, and 2 MIC)","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86881117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bahareh Musivand, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh
gliotoxin production, cell membrane ergosterol content, ultrastructure, and gliP gene expression were evaluated in the fungus exposed to 0.5× MIC concentrations of EAC (1000 μg/mL) and fluconazole (FCZ: 64 μg/mL). Findings: Ergosterol content was significantly reduced to 0.53 and 0.45 µg/mg in EAC-and FCZ-treated fungal cells, respectively ( p < .001). The protease activity was significantly inhibited in both EAC- and FCZ-treated groups. The gliotoxin production was inhibited by 51.55 and 68.75% in the treated groups with FCZ and EAC, respectively. The expression of gliP in both EAC- and FCZ-treated was significantly reduced by 0.40 and 0.53-fold, respectively ( p < .05). Conclusion: This study findings revealed that growth and virulence bioactive Further are recommended to identify
{"title":"Allium cepa Inhibits Aspergillus fumigatus Growth and Virulence and Suppresses the Expression of glip Gene Involved in Fungal Pathogenesis","authors":"Bahareh Musivand, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh","doi":"10.52547/iem.8.2.149","DOIUrl":"https://doi.org/10.52547/iem.8.2.149","url":null,"abstract":"gliotoxin production, cell membrane ergosterol content, ultrastructure, and gliP gene expression were evaluated in the fungus exposed to 0.5× MIC concentrations of EAC (1000 μg/mL) and fluconazole (FCZ: 64 μg/mL). Findings: Ergosterol content was significantly reduced to 0.53 and 0.45 µg/mg in EAC-and FCZ-treated fungal cells, respectively ( p < .001). The protease activity was significantly inhibited in both EAC- and FCZ-treated groups. The gliotoxin production was inhibited by 51.55 and 68.75% in the treated groups with FCZ and EAC, respectively. The expression of gliP in both EAC- and FCZ-treated was significantly reduced by 0.40 and 0.53-fold, respectively ( p < .05). Conclusion: This study findings revealed that growth and virulence bioactive Further are recommended to identify","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88643596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Esmaeilzadeh, S. Sabzi, Aida Hajihossein Tabrizi
Specific Bacteriophages against Methicil-lin-Resistant Bacteriophage therapy could be an alternative strategy for the treatment of antibiotic-resistant bacteria. This study aimed to evaluate the antibacterial activities of isolated bacteriophages against methicillin-resistant Staphylococcus aureus (MRSA) isolates. Materials & Methods: A total of 16 clinical isolates of MRSA were collected from medical diagnostic laboratories in Tehran, Iran. A specific bacteriophage was isolated from hospital sewage using double-layer agar. Phage morphology was evaluated by transmission electron microscopy (TEM). Different bacteria were selected to determine the bacteriophage host range using spot test. Phage susceptibility to temperature and pH was evaluated by double-layer agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the adhesion of MRSA to human epithelial cells. Findings : TEM suggested the Myoviridae family for the isolated phage. The effective titer of bacteriophages was 1.8×10 The bacteriophage 4 and pH=8. The isolated bacteriophage was specific for all clinical isolates of MRSA and had no lytic activity against other pathogenic bacteria. In evaluating the binding and invasion of MRSA to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophages was observed following inoculation. Conclusion: The specificity and lytic activity of this phage on MRSA and MRSA-infected HEp-2 cell line emphasized that the isolated bacteriophage may serve as an effective prophylactic and alternative therapeutic agent in hospital settings.
{"title":"Isolation and in Vitro Characterization of Specific Bacteriophages against Methicillin-Resistant Staphylococcus aureus","authors":"M. Esmaeilzadeh, S. Sabzi, Aida Hajihossein Tabrizi","doi":"10.52547/iem.8.2.121","DOIUrl":"https://doi.org/10.52547/iem.8.2.121","url":null,"abstract":"Specific Bacteriophages against Methicil-lin-Resistant Bacteriophage therapy could be an alternative strategy for the treatment of antibiotic-resistant bacteria. This study aimed to evaluate the antibacterial activities of isolated bacteriophages against methicillin-resistant Staphylococcus aureus (MRSA) isolates. Materials & Methods: A total of 16 clinical isolates of MRSA were collected from medical diagnostic laboratories in Tehran, Iran. A specific bacteriophage was isolated from hospital sewage using double-layer agar. Phage morphology was evaluated by transmission electron microscopy (TEM). Different bacteria were selected to determine the bacteriophage host range using spot test. Phage susceptibility to temperature and pH was evaluated by double-layer agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the adhesion of MRSA to human epithelial cells. Findings : TEM suggested the Myoviridae family for the isolated phage. The effective titer of bacteriophages was 1.8×10 The bacteriophage 4 and pH=8. The isolated bacteriophage was specific for all clinical isolates of MRSA and had no lytic activity against other pathogenic bacteria. In evaluating the binding and invasion of MRSA to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophages was observed following inoculation. Conclusion: The specificity and lytic activity of this phage on MRSA and MRSA-infected HEp-2 cell line emphasized that the isolated bacteriophage may serve as an effective prophylactic and alternative therapeutic agent in hospital settings.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73453408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saba Jalalifar, Tahereh Motallebirad, Shirin Dashtbin, Rasoul Mirzaei, M. Khorshidi, Bahram Nasr Esfahani
B antibiotics (cMLSB phenotype) has become a global concern. On the other hand, little is known about the genetic relatedness and diversity of GBS isolates isolated from various patients in Iran. Hence, this study aimed to determine the genetic relatedness and molecular typing of cMLSB-GBS isolates using enterobacterial repetitive intergenic consensus-PCR (ERIC- PCR) technique. Materials & Methods: A total of 100 GBS isolates were collected from patients with urinary tract infections (UTIs). Among them, 52 erythromycin-resistant GBS isolates were selected, and double-disc diffusion (D-zone) technique was applied to determine the MLSB phenotype among the isolates based on CLSI criteria. Then the genetic relatedness of MLSB-GBS isolates was assessed using ERIC-PCR fingerprinting method. Findings: Among 52 erythromycin-resistant GBS isolates, 38 isolates were identified with cMLSB phenotype, nine isolates with M phenotype, and five isolates with iMLSB phenotype. The analysis of ERIC-PCR patterns revealed eight different ERIC types that were divided into seven clusters (A-G) and one single type. Also, four isolates were non-typeable. ERIC type A/ serotype Ib was the most prevalent clone among the isolates. Conclusion: The current study findings showed a high level of diversity and multiclonal spread of the cMLSB phenotype in Isfahan. ERIC type A/ serotype Ib is the predominant clone circulating among erythromycin-resistant GBS strains.
{"title":"Molecular Typing of Streptococcus agalactiae- cMLSB Phenotype Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) in Isfahan, Iran","authors":"Saba Jalalifar, Tahereh Motallebirad, Shirin Dashtbin, Rasoul Mirzaei, M. Khorshidi, Bahram Nasr Esfahani","doi":"10.52547/iem.8.2.139","DOIUrl":"https://doi.org/10.52547/iem.8.2.139","url":null,"abstract":"B antibiotics (cMLSB phenotype) has become a global concern. On the other hand, little is known about the genetic relatedness and diversity of GBS isolates isolated from various patients in Iran. Hence, this study aimed to determine the genetic relatedness and molecular typing of cMLSB-GBS isolates using enterobacterial repetitive intergenic consensus-PCR (ERIC- PCR) technique. Materials & Methods: A total of 100 GBS isolates were collected from patients with urinary tract infections (UTIs). Among them, 52 erythromycin-resistant GBS isolates were selected, and double-disc diffusion (D-zone) technique was applied to determine the MLSB phenotype among the isolates based on CLSI criteria. Then the genetic relatedness of MLSB-GBS isolates was assessed using ERIC-PCR fingerprinting method. Findings: Among 52 erythromycin-resistant GBS isolates, 38 isolates were identified with cMLSB phenotype, nine isolates with M phenotype, and five isolates with iMLSB phenotype. The analysis of ERIC-PCR patterns revealed eight different ERIC types that were divided into seven clusters (A-G) and one single type. Also, four isolates were non-typeable. ERIC type A/ serotype Ib was the most prevalent clone among the isolates. Conclusion: The current study findings showed a high level of diversity and multiclonal spread of the cMLSB phenotype in Isfahan. ERIC type A/ serotype Ib is the predominant clone circulating among erythromycin-resistant GBS strains.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83353357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Majidpour, Nastaran Arianpor, Sara Fathizadeh, Samira Rasouli koohi, Somayeh Soleymanzadeh moghaddam
they were hospitalized in Rasoul Akram hospital in Iran from August 2017 to February 2018. Patients’ demographic and laboratory data, such as RDW (red cell distribution width), PDW (platelet distribution width), RBC (red blood cell), CRP (C-reactive protein), ESR (erythrocyte sedimentation rate), and, WBC (white blood cells), were evaluated. Findings: This study results showed that mortality rate in sepsis cases was higher than in other cases (42.1%). Changes in blood parameters such as RDW, PDW, and EDR levels as well as monocyte, basophil, and eosinophil counts were significant among patients with different infectious diseases, while there was no significant difference in terms of changes in some blood parameters, such as WBC, neutrophil, and lymphocyte counts and CRP level between patients with different infectious diseases. For statistical analysis, one-way ANOVA and LSD post hoc tests were used. Conclusion: According to this study results, it was found that the range of blood parameters varies in different types of infectious diseases. Therefore, the could employ routine blood parameters along with other diagnostic factors to more accurately diagnose the type of infection prescribe appropriate
{"title":"Association between Hematology Indices as Early Markers and Urinary Tract Infection, Septicemia, Pneumonia, and Diabetic Foot Infection","authors":"A. Majidpour, Nastaran Arianpor, Sara Fathizadeh, Samira Rasouli koohi, Somayeh Soleymanzadeh moghaddam","doi":"10.52547/iem.8.1.7","DOIUrl":"https://doi.org/10.52547/iem.8.1.7","url":null,"abstract":"they were hospitalized in Rasoul Akram hospital in Iran from August 2017 to February 2018. Patients’ demographic and laboratory data, such as RDW (red cell distribution width), PDW (platelet distribution width), RBC (red blood cell), CRP (C-reactive protein), ESR (erythrocyte sedimentation rate), and, WBC (white blood cells), were evaluated. Findings: This study results showed that mortality rate in sepsis cases was higher than in other cases (42.1%). Changes in blood parameters such as RDW, PDW, and EDR levels as well as monocyte, basophil, and eosinophil counts were significant among patients with different infectious diseases, while there was no significant difference in terms of changes in some blood parameters, such as WBC, neutrophil, and lymphocyte counts and CRP level between patients with different infectious diseases. For statistical analysis, one-way ANOVA and LSD post hoc tests were used. Conclusion: According to this study results, it was found that the range of blood parameters varies in different types of infectious diseases. Therefore, the could employ routine blood parameters along with other diagnostic factors to more accurately diagnose the type of infection prescribe appropriate","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72790813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
more effectively improve the implantation and targeting of the colon cancer cell model. Materials & Methods: In this study, the structure of the CD44 gene was designed in lentiviral vectors and transfected to the HEK293T cell line along with auxiliary plasmids PSPAX2 and PMDG2. The growth medium of virus-containing cells was collected at optimized intervals, and transduction into mice mesenchymal stem cells, injection into mice, and homing processes were traced. Findings: Successful production of lentiviral vectors and proper expression of the corresponding factor after transduction were effective in improving the MSC homing in cancer cell. Findings: According to these findings, it could be suggested that high expression of CD44v6 factor could be effective in improving the implantation process in cancer cells and targeting treatment.
{"title":"An Engineered Mesenchymal Stem Cell by Lentiviral Vector Expressing CD44 as a Candidate to Target Colon Cancer Tissue in Mice Model","authors":"A. Nabizadeh, M. Ravanshad","doi":"10.52547/iem.8.1.69","DOIUrl":"https://doi.org/10.52547/iem.8.1.69","url":null,"abstract":"more effectively improve the implantation and targeting of the colon cancer cell model. Materials & Methods: In this study, the structure of the CD44 gene was designed in lentiviral vectors and transfected to the HEK293T cell line along with auxiliary plasmids PSPAX2 and PMDG2. The growth medium of virus-containing cells was collected at optimized intervals, and transduction into mice mesenchymal stem cells, injection into mice, and homing processes were traced. Findings: Successful production of lentiviral vectors and proper expression of the corresponding factor after transduction were effective in improving the MSC homing in cancer cell. Findings: According to these findings, it could be suggested that high expression of CD44v6 factor could be effective in improving the implantation process in cancer cells and targeting treatment.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"312 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85384465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abozar Nasiri-Jahrodi, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh
Backgrounds: Aspergillus fumigatus is a pathogen responsible for invasive aspergillosis and the main leading cause of death in immunosuppressed individuals. The present study aimed to evaluate the impact of eugenol-loaded chitosan nanoparticles on the expression of CYP51a and CYP51b , two well-known genes responsible for triazole drug resistance in A. fumigatus . The minimum inhibitory concentration (MIC) of eugenol-loaded chitosan nanoparticles, chitosan, eugenol, and itraconazole was determined based on the Clinical and Laboratory Standards Institute M38-E3 method at concentrations of 4.6-2400, 11.7-12000, 2-2048, and 1-256 μ g/mL, respectively. The expression of CYP51A and CYP51B was evaluated in A. fumigatus exposed to 0.5, 1, and 2× of MIC concentration of NPs and itraconazole using the real-time polymerase chain reaction. Findings: The obtained results showed that eugenol-loaded chitosan nanoparticles sucessfully reduced eugenol, and itraconazole was measured to be 6000, 256, and 4 μg/mL, respectively. The results of real-time PCR also revealed that eugenol-loaded chitosan nanoparticles increased the expression of both CYP51A and CYP51B in a dose-dependent manner. The expression of fungal CYP51A and CYP51B at mRNA level was significantly increased 1.26, 1.93, and 3.1-fold as well as 1.2, 2.1, and 2.4-fold at concentrations of 150, 300, and 600 μg/mL , respectively ( p <.05). However, it seems that the prepared nanoparticles had a lower impact on the expression of these genes compared to itraconazole. findings the treatment of with eugenol-chitosan nanoparticles increase the of the CYP51 suggesting the antifungal property of these nanoparticles.
{"title":"Effects of Eugenol-Loaded Chitosan Biopolymer Nanoparticles on CYP51A and CYP51B Expression in Aspergillus fumigatus","authors":"Abozar Nasiri-Jahrodi, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh","doi":"10.52547/iem.8.1.17","DOIUrl":"https://doi.org/10.52547/iem.8.1.17","url":null,"abstract":"Backgrounds: Aspergillus fumigatus is a pathogen responsible for invasive aspergillosis and the main leading cause of death in immunosuppressed individuals. The present study aimed to evaluate the impact of eugenol-loaded chitosan nanoparticles on the expression of CYP51a and CYP51b , two well-known genes responsible for triazole drug resistance in A. fumigatus . The minimum inhibitory concentration (MIC) of eugenol-loaded chitosan nanoparticles, chitosan, eugenol, and itraconazole was determined based on the Clinical and Laboratory Standards Institute M38-E3 method at concentrations of 4.6-2400, 11.7-12000, 2-2048, and 1-256 μ g/mL, respectively. The expression of CYP51A and CYP51B was evaluated in A. fumigatus exposed to 0.5, 1, and 2× of MIC concentration of NPs and itraconazole using the real-time polymerase chain reaction. Findings: The obtained results showed that eugenol-loaded chitosan nanoparticles sucessfully reduced eugenol, and itraconazole was measured to be 6000, 256, and 4 μg/mL, respectively. The results of real-time PCR also revealed that eugenol-loaded chitosan nanoparticles increased the expression of both CYP51A and CYP51B in a dose-dependent manner. The expression of fungal CYP51A and CYP51B at mRNA level was significantly increased 1.26, 1.93, and 3.1-fold as well as 1.2, 2.1, and 2.4-fold at concentrations of 150, 300, and 600 μg/mL , respectively ( p <.05). However, it seems that the prepared nanoparticles had a lower impact on the expression of these genes compared to itraconazole. findings the treatment of with eugenol-chitosan nanoparticles increase the of the CYP51 suggesting the antifungal property of these nanoparticles.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76900439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Rezaei, F. Javanmardi, Nastaran Shahriari, N. Pirbonyeh, A. Emami, Hamid Zare
to the laboratory for culture and biochemical tests. After accurate identification of bacterial agents, antibiotic susceptibility of all isolated bacteria was evaluated by disk diffusion method based on CLSI guidelines. Data were analyzed using SPSS software (Version 19). Findings: In this study, 166 patients with diabetic foot ulcers were evaluated. The mean age of patients was 55.8± 13.2 years, and 109 (66.4%) cases were male. Also, 62% of patients had an underlying disease, while most of them had hypertension (27%). The most prevalent isolated bacterium was Staphylococcus epidermidis . The most effective antibiotics against isolated Gram-positive and Gram-negative bacteria were vancomycin and amikacin, respectively. Conclusion: In this study, it was concluded that the frequency of Gram-negative bacteria in diabetic foot ulcer infections was higher than that of Gram-positive bacteria.
{"title":"Bacterial Etiology and Antibiotic Resistance Pattern of Diabetic Foot Infection in Patients Admitted to Shiraz Hospitals, Iran","authors":"A. Rezaei, F. Javanmardi, Nastaran Shahriari, N. Pirbonyeh, A. Emami, Hamid Zare","doi":"10.52547/iem.8.1.1","DOIUrl":"https://doi.org/10.52547/iem.8.1.1","url":null,"abstract":"to the laboratory for culture and biochemical tests. After accurate identification of bacterial agents, antibiotic susceptibility of all isolated bacteria was evaluated by disk diffusion method based on CLSI guidelines. Data were analyzed using SPSS software (Version 19). Findings: In this study, 166 patients with diabetic foot ulcers were evaluated. The mean age of patients was 55.8± 13.2 years, and 109 (66.4%) cases were male. Also, 62% of patients had an underlying disease, while most of them had hypertension (27%). The most prevalent isolated bacterium was Staphylococcus epidermidis . The most effective antibiotics against isolated Gram-positive and Gram-negative bacteria were vancomycin and amikacin, respectively. Conclusion: In this study, it was concluded that the frequency of Gram-negative bacteria in diabetic foot ulcer infections was higher than that of Gram-positive bacteria.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73047735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：