Journal of Chromatography A最新文献_第9页

Recoverable and reusable light-induced multi-arm azobenzenes-Fe3O4 hybrid sorbent for enrichment of phthalate plasticizer and utilized as a SALDI substrate for the detection of 2-naphthol 可回收和重复使用的光诱导多臂偶氮苯-Fe3O4 混合吸附剂，用于富集邻苯二甲酸酯类增塑剂，并用作检测 2-萘酚的 SALDI 底物。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-05 DOI: 10.1016/j.chroma.2024.465418

Abdullah Alhendal , Mohamed Rashad , Laila Alshatti , Fouzi Mouffouk , Ali Husain , Abrar Alrashed

The construction, structural identifications along with compositional properties, using TEM, FT-IR, and XPS spectroscopies, of an innovative light-induced multi-arm azobenzenes based Fe₃O₄ magnetic nanoparticles (Azo-Fe₃O₄ MNPs) are being reported. Such organic (light-sensitive dendrimers-like structure), inorganic (magnet core), hybrid material has been applied as an efficient recoverable/reusable extractive sorbent for the detection of phthalate plasticizers from acetate buffer solution. The extraction study was controlled within consecutive procedures via UV-light exposure to achieve pore-size control which then further subjected for the evaluation of the analytes’ retention, separation, and release as well as the detection of the phthalate pollutants using GCMS–. Various experimental conditions, such as time of extraction, salt concentration, pH, and desorption time, were studied and adjusted. Additionally, the extraction repeatability (RSD from 0.46 % to 6.12%, n = 5) of the studied sorbent was comparable to other published work. The linear range extended from 6.25 to 100 μg L^-¹ and detection limits (LOD) within the range of 41- 150 ng L^-1 were achieved, demonstrating good linearity with values ranging from 0.9992 to 0.9892. The inter-batch and intra-batch RSD ranged from 0.46 % to 6.12 %, respectively. Additionally, it provides effective detection of 2-naphthol when used as a SALDI substrate.

本报告利用 TEM、FT-IR 和 XPS 光谱对一种创新的光诱导多臂偶氮苯基 Fe3O4 磁性纳米粒子（Azo-Fe3O4 MNPs）进行了构建、结构鉴定和成分特性分析。这种有机（类光敏树枝状聚合物结构）、无机（磁芯）混合材料已被用作一种高效的可回收/可重复使用的萃取吸附剂，用于检测醋酸盐缓冲溶液中的邻苯二甲酸酯类增塑剂。萃取研究通过紫外光照射进行连续控制，以实现孔径控制，然后进一步评估分析物的保留、分离和释放情况，并使用 GCMS 检测邻苯二甲酸酯污染物。研究并调整了各种实验条件，如萃取时间、盐浓度、pH 值和解吸时间。此外，所研究的吸附剂的萃取重复性（RSD 从 0.46 % 到 6.12%，n = 5）与其他已发表的研究成果相当。线性范围从 6.25 μg L-¹ 扩展到 100 μg L-¹，检出限（LOD）在 41-150 ng L-1 之间，线性范围为 0.9992 到 0.9892。批间和批内 RSD 分别为 0.46 % 至 6.12 %。此外，在用作 SALDI 底物时，它还能有效检测 2-萘酚。

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引用次数: 0

Maximizing sensitivity and selectivity in LC × LC-HRMS for pesticide analysis via exploitation of per-aqueous liquid chromatography 利用过水液相色谱法最大限度提高用于农药分析的 LC × LC-HRMS 的灵敏度和选择性

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-05 DOI: 10.1016/j.chroma.2024.465403

Elena Bandini , Stephanie A. Schuster , Turaj Rahmani , Frédéric Lynen

Accurate monitoring of pesticide residues at minimal concentrations is imperative for adherence to stringent regulatory standards in numerous countries. This study presents an innovative methodology employing comprehensive two-dimensional liquid chromatography coupled with high-resolution mass spectrometry (LC × LC-HRMS). The approach ensures high sensitivity and selectivity in detecting targeted compounds. A pivotal component of this methodology is the utilization of per-aqueous liquid chromatography (PALC) as the first dimension, facilitating the use of water-based mobile phases and addressing solvent mismatch issues. The second dimension employs reversed-phase liquid chromatography (RPLC), enhancing the separation of compounds. PALC proves instrumental in refocusing and enables the practical application of narrow-diameter columns (1.5 mm I.D.). This column design permits a direct split-free connection of the LC × LC to an electrospray-based mass spectrometer (ESI-MS), contributing to heightened sensitivity. The MS acquisition is performed in a targeted single-ion monitoring mode, ensuring reliable quantification and identification of the pesticide compounds. A comprehensive evaluation of key performance metrics, including signal-to-noise ratio, limit of detection, and response linearity, is conducted. The methodology achieves a limit of detection below the ng mL⁻¹ and exhibits response linearity within the concentration range of 1–100 ng mL⁻¹. The robustness of the approach is further demonstrated through intra-day and inter-day repeatability validations. Furthermore, the platform is finally tested on a surface water sample. This study not only introduces an advanced analytical methodology for pesticide multi-residue analysis but also underscores the significance of PALC in enhancing sensitivity by facilitating the use of smaller-diameter columns and water-based mobile phases, along with the role of RPLC in enhancing separation. The proposed approach showcases promising results in achieving detection limits that match the stringent regulatory standards and reliable quantification for effective pesticide residue monitoring.

在许多国家，要遵守严格的监管标准，就必须对最低浓度的农药残留进行精确监测。本研究提出了一种创新方法，采用全面的二维液相色谱法和高分辨质谱法（LC × LC-HRMS）。该方法可确保高灵敏度和高选择性地检测目标化合物。该方法的一个关键组成部分是将过水液相色谱法（PALC）作为第一维，从而方便了水基流动相的使用，并解决了溶剂不匹配的问题。第二个维度采用反相液相色谱 (RPLC)，提高了化合物的分离度。事实证明，PALC 有助于重新聚焦，并能实际应用窄直径色谱柱（内径 1.5 毫米）。这种色谱柱设计允许 LC × LC 与电喷雾质谱仪（ESI-MS）直接无拆分连接，从而提高了灵敏度。质谱采集以目标单离子监测模式进行，确保了农药化合物的可靠定量和鉴定。对关键性能指标进行了全面评估，包括信噪比、检测限和响应线性度。该方法的检测限低于纳克毫升-1，在 1-100 纳克毫升-1 的浓度范围内呈线性响应。通过日内和日间重复性验证，进一步证明了该方法的稳健性。此外，该平台最后还在地表水样本上进行了测试。这项研究不仅为农药多残留分析引入了一种先进的分析方法，还强调了 PALC 在提高灵敏度方面的重要作用，因为它有利于使用小直径色谱柱和水基流动相，同时还强调了 RPLC 在提高分离度方面的作用。所提出的方法在实现符合严格监管标准的检测限和可靠定量方面取得了可喜的成果，可用于有效的农药残留监测。

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引用次数: 0

Zirconium(IV) coordination-mediated rapid and versatile post-modification of polydopamine coating as stationary phase for open-tubular capillary electrochromatography 锆（IV）配位介导的聚多巴胺涂层快速、多功能后改性，作为开放管式毛细管电色谱的固定相。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-02 DOI: 10.1016/j.chroma.2024.465415

Xueping Tao , Mengting Su , Panpan Chen , Meiting Yan , Dan Wang , Lan Xia , Li Rao , Zhining Xia , Qifeng Fu

In recent years, mussel-inspired polydopamine (PDA)-based materials have attracted significant attention in the field of open-tubular capillary electrochromatography (OT-CEC) owing to their diverse and appealing properties. However, previously established functionalized PDA coating-based CEC stationary phases predominantly relied on the latent reactivity of PDA with amine/thiol-containing molecules, limiting the types of applicable modifiers and requiring time-consuming reaction processes. Herein, we presented a versatile and efficient method for the facile and rapid fabrication of diverse functionalized PDA coatings as OT-CEC stationary phases through a Zr(IV) coordination-mediated post-modification strategy. Different kinds of modifiers, including octadecylamine (ODA), lauric acid (LA), and perfluorooctanoic acid (PFOA), were rapidly and robustly grafted onto the PDA coating, verified through multiple characterization techniques. The influences of preparation parameters on the grafting efficiency of the functionalized PDA coating were systematically investigated. Utilizing the Zr(IV)-mediated ODA-, LA- and PFOA-functionalized PDA-based OT-CEC columns, we achieved high-efficiency baseline separation of a series of neutral analytes with excellent repeatability, good stability, and long lifetime. Given the strong universality of the Zr(IV) coordination-mediated post-modification approach, our study provides an effective pathway for advancing the development of a wider range of functional PDA-based chromatographic stationary phases.

近年来，基于贻贝启发的聚多巴胺（PDA）材料因其多样化的诱人特性，在开放管状毛细管电色谱（OT-CEC）领域引起了极大关注。然而，以前建立的基于 PDA 涂层的功能化 CEC 固定相主要依赖于 PDA 与含胺/硫醇分子的潜在反应性，从而限制了适用改性剂的类型，并且需要耗时的反应过程。在此，我们提出了一种多功能、高效的方法，通过 Zr（IV）配位介导的后改性策略，方便、快速地制备出多种功能化 PDA 涂层，作为 OT-CEC 固定相。不同种类的改性剂，包括十八胺（ODA）、月桂酸（LA）和全氟辛酸（PFOA），被快速而牢固地接枝到 PDA 涂层上，并通过多种表征技术进行了验证。系统研究了制备参数对功能化 PDA 涂层接枝效率的影响。利用 Zr(IV)介导的 ODA、LA 和 PFOA 功能化 PDA 基 OT-CEC 色谱柱，我们实现了一系列中性分析物的高效基线分离，并具有优异的重复性、良好的稳定性和较长的使用寿命。鉴于 Zr（IV）配位介导的后修饰方法具有很强的通用性，我们的研究为推动更广泛的基于 PDA 的功能性色谱固定相的开发提供了有效途径。

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引用次数: 0

Peptide retention time prediction for electrostatic repulsion-hydrophilic interaction chromatography 静电排斥-亲水相互作用色谱法的肽保留时间预测。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-02 DOI: 10.1016/j.chroma.2024.465414

Quinn Neale , Darien Yeung , Victor Spicer , Helene Perreault , Oleg Krokhin

Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) is one of the legacy separation tools developed by Dr. Andrew Alpert and has been used for developing unique separation methods of hydrophilic compounds, including peptides. In the past it has been studied using designed peptide libraries to elucidate major features of its separation mechanism, while comprehensive peptide retention modeling for ERLIC is still lacking. In this work we employed a proteomics-derived ∼170,000 peptide retention datasets to evaluate major ERLIC retention features using the framework of our Sequence-Specific Retention Calculator model. The separation conditions were adjusted to obtain a wider proteome coverage, particularly for non-modified peptides, resulting in a superior separation orthogonality for a 2D LC combination with reversed-phase C18 LC-MS in the second dimension. The SSRCalc ERLIC model presents a consistent theme with the existing ERLIC retention mechanism, reflecting a dependence on peptide orientation and the position of charged and hydrophilic residues across the peptide backbone. R² values of 0.935 and 0.955 accuracy were demonstrated for the standard interpretable SSRCalc model and machine learning algorithm, respectively. The effects of various PTMs on peptide retention were evaluated in this study, covering spontaneous (oxidation, deamidation) and enzymatic (N-terminal acetylation, phosphorylation, glycosylation) modifications.

静电斥力-亲水相互作用色谱法（ERLIC）是安德鲁-阿尔伯特博士开发的传统分离工具之一，一直用于开发包括肽在内的亲水性化合物的独特分离方法。过去，人们利用设计的肽库对其进行研究，以阐明其分离机制的主要特征，但仍缺乏针对 ERLIC 的全面的肽保留模型。在这项工作中，我们利用蛋白质组学衍生的 17 万个肽段保留数据集，在序列特异性保留计算模型的框架内评估了 ERLIC 的主要保留特征。对分离条件进行了调整，以获得更广泛的蛋白质组覆盖范围，特别是非修饰肽段，从而使二维液相色谱与反相 C18 LC-MS 在二维中的分离正交性更优越。SSRCalc ERLIC 模型与现有的 ERLIC 保留机制呈现出一致的主题，反映了肽的取向以及肽骨架上带电和亲水残基位置的依赖性。标准可解释 SSRCalc 模型和机器学习算法的 R2 值分别为 0.935 和 0.955。本研究评估了各种 PTM 对肽保留的影响，包括自发修饰（氧化、脱氨基）和酶修饰（N 端乙酰化、磷酸化、糖基化）。

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引用次数: 0

A simple method to remove polyimide coating from fused silica capillaries for capillary electrophoresis 从毛细管电泳用熔融石英毛细管上去除聚酰亚胺涂层的简单方法。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-01 DOI: 10.1016/j.chroma.2024.465395

Pan Wang, Yulin Cheng, Wei Wei

A simple and cost-effective method for making capillary electrophoresis (CE) detection windows is reported. A low-cost laser engraving machine equipped with a 450 nm high-power blue laser was used to remove the polyimide coating from the fused silica capillary by laser scanning the desired section. The entire process is automated and controlled in real time by software. This method can be easily adopted by many CE users for routine practice and is scalable for high-throughput production of detection windows across multiple capillaries.

本报告介绍了一种制作毛细管电泳（CE）检测窗的简单而经济的方法。使用配备 450 nm 高功率蓝色激光的低成本激光雕刻机，通过激光扫描所需的部分，去除熔融石英毛细管上的聚酰亚胺涂层。整个过程由软件自动实时控制。这种方法很容易被许多 CE 用户用于日常实践，并可扩展用于多个毛细管检测窗口的高通量生产。

引用次数: 0

An explore method for quick screening biomarkers based on effective enrichment capacity and data mining 基于有效富集能力和数据挖掘的生物标记物快速筛选探索方法。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-10-01 DOI: 10.1016/j.chroma.2024.465413

Hongli Zhao , Yifan Zhou , Qinying Gu , Yunfan Lin , Minbo Lan

Protein glycosylation acts as a crucial role in regulating protein function and maintaining cellular homeostasis. Efficient peptide enrichment can be utilized to effectively solve the inherent challenges of protein glycosylation analysis to search unknown cancer biomarkers. In this research, a low dimensional porous hydrophilic nanosheets with a multi-level porous structure (Co-MOF-SiO₂@HA) was synthetized via an easy one-pot method for the efficient enrichment of the N-glycopeptides in the digests of complex biosamples. The synthetized nanosheets Co-MOF-SiO₂@HA demonstrated excellent enriching performances including a high enrichment capacity (300 mg g^-1 calculated), a spectacular selectivity (IgG digests and BSA digests at the molar ratio of 1/1200), and an excellent spatial confinement ability (IgG digests, IgG and BSA at the molar ratio of 1/1000/1000). As an explore result, after the enrichment of human colorectal cancer tissue and human healthy tissue by the nanosheets, several proteins related to cancers and one protein directly related to well-known human colorectal cancer were identified by detecting the corresponding glycopeptides. It presented the potential value of the feasibility of this analysis mode by nanosheets Co-MOF-SiO₂@HA in proteomic analysis.

蛋白质糖基化在调节蛋白质功能和维持细胞稳态方面起着至关重要的作用。利用高效的多肽富集技术可以有效解决蛋白质糖基化分析的固有难题，从而寻找未知的癌症生物标志物。本研究通过简单的一锅法合成了具有多层次多孔结构的低维多孔亲水纳米片（Co-MOF-SiO2@HA），用于高效富集复杂生物样品消化液中的N-糖肽。所合成的纳米片 Co-MOF-SiO2@HA 具有优异的富集性能，包括高富集能力（计算值为 300 mg g-1）、惊人的选择性（IgG 消化物和 BSA 消化物的摩尔比为 1/1200）以及出色的空间限制能力（IgG 消化物、IgG 和 BSA 的摩尔比为 1/1000/1000）。纳米片富集人体结直肠癌组织和人体健康组织后，通过检测相应的糖肽，发现了几种与癌症相关的蛋白质和一种与众所周知的人体结直肠癌直接相关的蛋白质。这说明纳米片Co-MOF-SiO2@HA这种分析模式在蛋白质组学分析中的可行性和潜在价值。

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引用次数: 0

Robustness evaluation of weak anion exchange chromatography method for the purity analysis of therapeutic oligonucleotides 用于治疗性寡核苷酸纯度分析的弱阴离子交换色谱法的稳健性评估。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465412

Lara Nercessian, Dilhan Ozturk, Claire I. Butré, Vincent Bertholet, Sébastien Patiny, Damien Mouvet, Arnaud Delobel

Therapeutic oligonucleotides are becoming an important class of therapeutics. Their manufacturing processes can result in the formation of impurities, particularly truncated species. To ensure the quality and safety of the product, it is crucial to evaluate the presence of these species. Liquid chromatography analysis enables such purity determination. In this context, a recently described weak anion exchange chromatography method was optimized to allow the effective separation of different impurities. The optimization addressed the complexity and instability of the mobile phases, which contained salts and organic compounds. Adjustments were made to the mobile phase composition and gradient to meet the requirements of QC laboratories. Additionally, to ensure the method's reliability, a robustness study was conducted based on a risk assessment. Five factors were considered potential risks and were assessed experimentally on different chromatographic outputs. This led to the definition of a robust space, ensuring the method's reliability for the purity determination of oligonucleotides.

治疗性寡核苷酸正在成为一类重要的治疗药物。其生产过程会产生杂质，尤其是截短的核苷酸。为确保产品的质量和安全性，评估这些物种的存在至关重要。液相色谱分析可以进行此类纯度测定。在这种情况下，对最近描述的弱阴离子交换色谱法进行了优化，以有效分离不同的杂质。这次优化解决了流动相的复杂性和不稳定性问题，流动相中含有盐和有机化合物。对流动相成分和梯度进行了调整，以满足质量控制实验室的要求。此外，为确保该方法的可靠性，还在风险评估的基础上进行了稳健性研究。五个因素被视为潜在风险，并在不同的色谱结果上进行了实验评估。最终确定了稳健空间，确保了寡核苷酸纯度测定方法的可靠性。

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引用次数: 0

Magnetic covalent organic framework as an absorbent coupled with a portable mass spectrometer for rapid detection of malachite green and its metabolite residues 磁性共价有机框架作为吸收剂与便携式质谱仪联用，用于快速检测孔雀石绿及其代谢物残留。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465407

Shanshan Zhu , Tianliang Wang , Qing Xu , Yongxiang Wu , Ning Gan

It is important to develop specific adsorbents for malachite green and other fish drug residues. Herein, a simple strategy for synthesizing a novel magnetic covalent organic frameworks (rFe₃O₄@Py-COF) has been studied, and the materials were used as a magnetic absorbent for solid phase extraction (MSPE) of malachite green (MG) and its metabolite as leucomalachite green (LMG) in fishes. In this study, the mild reduction program of formic acid replacing traditional sodium borohydride as a reducing agent has been adopted to increase the stability of the framework, which can maintain the original high crystallinity and surface area of the reduced COF. The secondary amine bond is expected to be used as the reaction center for further functionalization of COF pore wall. Subsequently, rFe₃O₄@Py-COF (rmCOF) obtained after reduction was used as MSPE materials to detect MG and LMG by a portable mass spectrometer. After optimizing the conditions, the linearity is good within the range of 1.25∼100 μg/kg (R²≥0.9954), the limits of detection (LODs) are 0.31∼0.44 μg/kg with satisfactory recovery (85.0 %∼106.0 %). These results indicate that the assay is suitable for monitoring MG and LMG in complex aquatic foods, providing protection for food safety.

开发针对孔雀石绿和其他鱼类药物残留的特异性吸附剂非常重要。本文研究了一种合成新型磁性共价有机框架（rFe3O4@Py-COF）的简单策略，并将该材料用作磁性吸附剂，用于固相萃取（MSPE）鱼类中的孔雀石绿（MG）及其代谢产物白孔雀石绿（LMG）。本研究采用甲酸温和还原程序取代传统的硼氢化钠作为还原剂，以提高框架的稳定性，从而保持还原 COF 原有的高结晶度和比表面积。仲胺键有望成为 COF 孔壁进一步官能化的反应中心。随后，还原后得到的 rFe3O4@Py-COF （rmCOF）被用作 MSPE 材料，通过便携式质谱仪检测 MG 和 LMG。优化条件后，在 1.25 ∼100 μg/kg 范围内线性关系良好（R2≥0.9954），检出限（LOD）为 0.31 ∼0.44 μg/kg，回收率为 85.0 % ∼106.0 %。这些结果表明，该检测方法适用于监测复杂水产食品中的 MG 和 LMG，为食品安全提供了保障。

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引用次数: 0

The emerging application of LC-based metallomics techniques to unravel the bioinorganic chemistry of toxic metal(loid)s 基于液相色谱的金属组学技术在揭示有毒金属（loid）的生物无机化学方面的新兴应用。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465409

Negar Pourzadi, Jürgen Gailer

The on-going anthropogenic emission of toxic metal(loid) species into the environment contaminates the food supply and drinking water resources in various parts of the world. Given that inorganic pollutants cannot be degraded, their increased influx into the bloodstream of babies, children and pregnant women is inevitable. Since the ramifications of the ensuing environmental exposure on human health remain poorly defined, fundamentally new insight into their bioinorganic chemistry in organisms is urgently needed. Based on the flow of dietary constituents through organisms, the interaction of toxic metal(loid) species with biomolecules in the bloodstream deserve particular attention as they play an integral role in the mechanisms of their chronic toxicity. Gaining insight into these bioinorganic processes is hampered by the biological complexity of plasma/red blood cells and the low concentrations of the metal(loid) species of interest, but can be overcome by employing LC techniques hyphenated to atomic spectroscopic detectors (i.e. metallomics techniques). This perspective aims to highlight the potential of unconventional hyphenated separation modes to advance our understanding of the bioinorganic chemistry of toxic metal(loid) species in the bloodstream-organ system. Four examples are illustrated. The application of anion-exchange (AEX) and size-exclusion chromatography (SEC) provided new insight into the blood-based bioinorganic mechanisms that direct Cd²⁺ and MeHg⁺ to target organs. AEX chromatography also allowed to observe the formation of complexes between Hg²⁺ and MeHg⁺ with L-cysteine at pH 7.4, that are implicated in their organ uptake. Lastly, the application of reversed phase (RP) chromatography revealed a possible cytosolic mechanism by which N-acetyl-L-cysteine binds to MeHg⁺ in the presence of cytosolic glutathione (GSH). New insight into other bioinorganic processes may advance the regulatory framework to better protect public health.

人类不断向环境中排放有毒金属（loid）物种，污染了世界各地的食品供应和饮用水资源。由于无机污染物无法降解，它们越来越多地进入婴儿、儿童和孕妇的血液是不可避免的。由于环境接触无机污染物对人类健康的影响尚不明确，因此迫切需要从根本上对无机污染物在生物体内的生物无机化学作用有新的认识。基于膳食成分在生物体内的流动，有毒金属（loid）物种与血液中生物大分子的相互作用值得特别关注，因为它们在其慢性毒性机制中发挥着不可或缺的作用。由于血浆/红血细胞的生物复杂性和相关金属（loid）物种的低浓度，阻碍了对这些生物无机过程的深入了解，但可以通过采用与原子光谱检测器相连接的液相色谱技术（即金属组学技术）来克服这一问题。本文旨在强调非常规联用分离模式的潜力，以促进我们对血液-器官系统中有毒金属（loid）物种的生物无机化学的了解。本文列举了四个实例。阴离子交换色谱法（AEX）和尺寸排阻色谱法（SEC）的应用使我们对引导 Cd2+ 和 MeHg+ 进入靶器官的血液生物无机机制有了新的认识。AEX 色谱法还能观察到 Hg2+ 和 MeHg+ 在 pH 值为 7.4 时与 L-半胱氨酸形成的复合物，这与它们被器官吸收有关。最后，反相色谱法的应用揭示了一种可能的细胞膜机制，即在细胞膜谷胱甘肽（GSH）存在的情况下，N-乙酰-L-半胱氨酸与 MeHg+ 结合。对其他生物无机过程的新认识可能会推进监管框架，从而更好地保护公众健康。

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引用次数: 0

Reducing the water quenching processes using heavy water in capillary electrophoresis with fluorescence detection 利用荧光检测减少毛细管电泳中重水的水淬过程。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A

Pub Date : 2024-09-30 DOI: 10.1016/j.chroma.2024.465411

Lenka Mádi , Josef Kučera , Štěpán Káňa , Taťána Bržezická , Petr Táborský

Water, ubiquitous in analytical methods, is renowned for its fluorescence quenching properties, influencing techniques like fluorescence spectrophotometry or techniques with fluorescence detection. This study explores the impact of water (H₂O) substitution for heavy water (D₂O) on the fluorescence behavior of anthraquinones and anthracyclines. Anthraquinones and anthracyclines play crucial roles in pharmacy, serving as essential components in various therapeutic formulations, particularly in cancer treatment and other pharmacological interventions. Capillary electrophoresis (CE) with heavy water as the background electrolyte (BGE) solvent offers superior sensitivity to the separation and detection of these analytes. Experimental results demonstrate the improved detection limits and separation efficiency of selected anthraquinones rhein (RH), aloe-emodin (AE), and anthracyclines doxorubicin (DOX), epirubicin (EPI) and daunorubicine (DAU) in heavy water-based buffers, highlighting the potential of heavy water in advancing analytical chemistry.

水在分析方法中无处不在，因其荧光淬灭特性而闻名，影响着荧光分光光度法等技术或荧光检测技术。本研究探讨了水（H₂O）取代重水（D₂O）对蒽醌类和蒽环类化合物荧光行为的影响。蒽醌类和蒽环类化合物在药学中发挥着重要作用，是各种治疗配方，特别是癌症治疗和其他药物干预中的重要成分。以重水为背景电解质（BGE）溶剂的毛细管电泳（CE）为这些分析物的分离和检测提供了卓越的灵敏度。实验结果表明，在以重水为基础的缓冲液中，选定的蒽醌类化合物瑞香素（RH）、芦荟大黄素（AE）以及蒽环类化合物多柔比星（DOX）、表柔比星（EPI）和达乌诺比星（DAU）的检测限和分离效率都得到了提高，这凸显了重水在推进分析化学方面的潜力。

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引用次数: 0