Journal of Chromatography A最新文献

{"title":"Bioseparation using membrane chromatography: Innovations, and challenges","authors":"Guoqiang Chen ,&nbsp;Yinhua Wan ,&nbsp;Raja Ghosh","doi":"10.1016/j.chroma.2025.465733","DOIUrl":"10.1016/j.chroma.2025.465733","url":null,"abstract":"<div><div>The resin-based column continues to be the dominant incumbent in bioprocess chromatography. While alternative formats such as membrane-, monolith- and fiber-based chromatography are more visible than before, each still plays minor roles. The reasons for this are complex and some of these are explained in this paper. However, the fact remains that membrane chromatography has come a long way since its early days of development. The main advantage of membrane chromatography continues to be its convection dominant transport mechanism, the resultant benefit being fast and scalable separation. Also, resolution obtained with properly designed devices could be comparable or even better than resin-based chromatography. Significant progress has been made in new membrane development, membrane characterization, device design and novel applications development. A wider range of new membrane matrices, ligands, and ligand-matrix linking chemistries are now available. New membrane modules, formats, and process configurations have also helped improve membrane performance. However, some significant challenges still exist, and these need to be addressed if membrane chromatography is to become more mainstream in the field of bioprocessing. Also, membrane chromatography has significant potential for application in analytical separations and this space has hardly been explored. In this paper, the advances in the areas of membrane preparation, device design and process development are reviewed. A high-level cost analysis is presented and the role of process design in membrane chromatography is discussed.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465733"},"PeriodicalIF":3.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatography media and purification processes for complex and super-large biomolecules: A review","authors":"Lan Zhao ,&nbsp;Guanghui Ma","doi":"10.1016/j.chroma.2025.465721","DOIUrl":"10.1016/j.chroma.2025.465721","url":null,"abstract":"<div><div>The biopharmaceutical industry has been one of the most dynamic industries in the world. New biopharmaceuticals are constantly developed, especially for complex and super-large biomolecules including antibodies, virus-like particles (VLPs), viral vectors, DNA, mRNA, and are very promising in drugs, vaccines, cell and gene therapy. Due to complex and unstable structures, as well as low concentration, it is very difficult to purify these complex and super-large biomolecules. Chromatography is the most widely used purification technique in bioseparation, and chromatography media is the key material. This review gives a comprehensive analysis of chromatography media and purification processes for complex and super-large biomolecules. A detailed summary of tailor-made chromatography media is first provided, including particle size and its uniformity, pore structure, spacer arm and polymer grafting, and new ligands and special separation mechanisms. Then the current choices and trends of purification processes for vaccines, VLPs, DNA, mRNA, antibodies and modified therapeutic proteins are reviewed. Finally, a brief overview of continuous biochromatography and computer-assisted chromatographic method development is provided. We hope this review will provide some useful guidance for design of chromatography media and purification of complex biopharmaceuticals.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465721"},"PeriodicalIF":3.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ‘One-Shot’ strategy for preparative chiral sub/supercritical fluid chromatography with chiroptical detection accelerates purification and enhances characterization for drug discovery","authors":"John Reilly ,&nbsp;Marie-Anne Lozach ,&nbsp;Aldo Meishammer ,&nbsp;Marcel Reck ,&nbsp;Robin A. Fairhurst ,&nbsp;Jeffrey M. McKenna ,&nbsp;Christopher J. Welch","doi":"10.1016/j.chroma.2025.465720","DOIUrl":"10.1016/j.chroma.2025.465720","url":null,"abstract":"<div><div>The advantages of a streamlined SFC chiral workflow for preparative chromatographic resolution of the enantiomers of investigational new compounds for pharmaceutical discovery on 10–20 mg scale with on-line chiroptical detection is described. The workflow has been designed to supply milligram amounts of enantiopure material using a universal gradient elution approach. Implementation of a faster and more labor efficient chiral purification workflow was the primary objective, with the secondary objective being an on-line collection of stereochemical information for separated enantiomers with the return of weighed compounds with acceptable purity. Column selection is carried out by gradient analytical SFC screening of six columns in 20 mins, while preparative resolution is carried out in a single injection using an 8-minute gradient with on-line polarimetric detection to assign and confirm the unique optical rotations of each of the eluted enantiomers. Additionally, electrospray ionization mass spectrometry detection is employed to facilitate fraction collection as well as product identification. The new workflow was piloted over a several month period and has proved to be an effective strategy for small scale preparative enantioseparations. Although these smaller-scale chiral separations currently constitute only a sixth of submissions in our laboratory, the one-shot workflow offers clear advantages for rapidly providing enantiopure material for initial <em>in-vitro</em> testing, reducing purification and evaporation cycle times and labor requirements.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465720"},"PeriodicalIF":3.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic liquid-modified magnetic covalent organic framework for the extraction of four pyrethroids in traditional Chinese herbs","authors":"Sitian Gu ,&nbsp;Wenli Zhu ,&nbsp;Yufei Liu ,&nbsp;Huixiao Duo ,&nbsp;Qingli Yang ,&nbsp;Xiudan Hou","doi":"10.1016/j.chroma.2025.465719","DOIUrl":"10.1016/j.chroma.2025.465719","url":null,"abstract":"<div><div>Efficient enrichment of analytes and purification of matrices are crucial for the highly sensitive detection and monitoring of pesticides in traditional Chinese herbs. This work prepared magnetic ionic liquid-controlled covalent organic framework (IL-COF@Fe₃O₄) as the sorbent <em>via</em> a simple in-situ precipitation polymerization and thiolene “click” strategy. The IL-COF@Fe₃O₄ exhibited remarkable adsorption performance towards pyrethroids within 5 min. The adsorption of four pyrethroids on the surface of IL-COF@Fe₃O₄ was according with Langmuir model and pseudo-second-order kinetic model. The adsorption energies were theoretically calculated, which were permethrin&gt;cypermethrin&gt;fenvalerate&gt;bifenthrin. The modification of ILs improved extraction capacity mainly because of the interaction of imidazole and Cl or F and the pore size effect. This method was developed for the rapid extraction of four pyrethroids in <em>Codonopsis pilosula</em> and <em>Angelica sinensis</em>. The linear range was 0.05–200 μg L^-1. Matrix effects were ranging from -16.14% to 9.53%, indicating the strong matrix anti-interference ability of IL-COF@Fe₃O₄.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1743 ","pages":"Article 465719"},"PeriodicalIF":3.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of deaminated impurities from the desired oligonucleotides using supercritical fluid chromatography","authors":"Momoka Hayashida ,&nbsp;Risa Suzuki ,&nbsp;Shinnosuke Horie ,&nbsp;Junichi Masuda ,&nbsp;Takao Yamaguchi ,&nbsp;Satoshi Obika","doi":"10.1016/j.chroma.2025.465731","DOIUrl":"10.1016/j.chroma.2025.465731","url":null,"abstract":"<div><div>With recent advancements concerning the optimization of the analytical conditions, it is feasible to analyze polar molecules using supercritical fluid chromatography (SFC). In this study, the applicability of SFC is evaluated for analyzing 5-, 10-, 15-, and 18-mer oligonucleotides, and SFC is then applied to analyze deaminated products, which are side products generated during oligonucleotide synthesis. These side products are difficult to separate from the target oligonucleotide, with the difficulty varying depending on the deamination position and sequences, even when using ion-pair reversed-phase liquid chromatography (IP-RPLC), a common method for oligonucleotide analysis. Our results demonstrate that SFC, with octylamine as a modifier additive, can achieve sharp chromatographic peaks for 5-, 10-, 15-, and 18-mer oligonucleotides modified with 2′-<em>O</em>-methoxyethyl RNA (2′-MOE), regardless of the presence of the hydrophobic 4,4′-dimethoxytrityl (DMTr) group on the sequence. After optimization of the column oven temperature, modifier additive, and stationary phase, SFC successfully separated oligonucleotides with various numbers and positions of deamination from the target oligonucleotide. SFC exhibited different selectivities for DMTr-on and DMTr-off oligonucleotides compared with those for IP-RPLC, which indicates that SFC can serve as a valuable alternative tool for the purification and analysis of oligonucleotides.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465731"},"PeriodicalIF":3.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of nonylphenol isomers in sewage and sludge of waste water treatment plant by GC-FID-MS combined with deans switch","authors":"Lang Liu ,&nbsp;Jianyi Zhang ,&nbsp;Yanqin Zhao ,&nbsp;Xunan Ning","doi":"10.1016/j.chroma.2025.465717","DOIUrl":"10.1016/j.chroma.2025.465717","url":null,"abstract":"<div><div>Nonylphenols (NPs) are persistent endocrine-disrupting organic pollutant. Most studies focus on total NP content, with limited research on individual isomers. This study developed a method to separation and detection of 10 NP isomers using GC-FID-MS combined with Deans switch. It can effectively transfer NPs from the first gas chromatography column (DB-5MS) to the second column (CP-ChiraSil-DEXCB), achieving efficient separation of the 10 isomers. Sewage and sludge samples from wastewater treatment plants were prepared using solid-phase extraction (SPE) and solid-liquid extraction. NP isomers in sewage samples were enriched with a phenol-specific SPE column, rinsed with 10 mL methanol-water (v/v 2:8), and eluted with 3 mL methanol and 5 mL dichloromethane. NP isomers in sludge samples underwent three ultrasonic extractions with 30 mL ethyl acetate-dichloromethane (v/v 1:1). Concentrate the extract to 1 mL and then purified using an LC<img>NH2 column, rinsed with 9 mL of ethyl acetate-n-hexane (v/v 1:9), and eluted with 10 mL of ethyl acetate-dichloromethane (v/v 1:1) .The results showed that the recovery rates for NP isomers ranged from 70.82 % to 110.12 % in sewage (LOQ: 0.09–0.31 μg/L; RSD: 1.82 %-10.12 %) and from 72.82 % to 114.12 % in sludge (LOQ: 0.15–0.47 μg/kg; RSD: 0.23 %-11.28 %), complying with the USEPA standards (70 %-130 %). Application of the method to real samples detected all 10 NP isomers, with concentrations of 0.50–5.01 μg/L in sewage and 0.13–24.91 μg/g in sludge.This method provides a reliable approach for the detection of NP isomers in complex environmental matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465717"},"PeriodicalIF":3.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of veterinary drugs in foods of animal origin by QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)","authors":"Qianran Sun ,&nbsp;Jun Liu ,&nbsp;Yuan Gou ,&nbsp;Tieyuan Chen ,&nbsp;Xiaofang Shen ,&nbsp;Tao Wang ,&nbsp;Yongli Li ,&nbsp;Huizhen He ,&nbsp;Huidan Deng ,&nbsp;Yi Hua","doi":"10.1016/j.chroma.2025.465726","DOIUrl":"10.1016/j.chroma.2025.465726","url":null,"abstract":"<div><div>A method using QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of the residues of 19 veterinary drugs in ten animal-derived matrices, including beef, pork, sheep, horse, chicken, prawn, fish, liver, milk, and fat. This method was based on the enactment of veterinary drug compounds by Korea, Canada, the United States, and the European Union in recent years. The samples were extracted using 85% acetonitrile and separated on an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with a gradient elution of methanol-0.2% formic acid water as the mobile phase. The detection of the analytes was achieved through the use of positive ion electrospray ionization (ESI) and multiple reaction monitoring (MRM) modes, while the quantification was conducted via the matrix-matched external standard method. Following optimization, the linearity of the target veterinary residues in the ten matrices was observed to be satisfactory, having a range of 0.5–50.0 ng/mL (R² &gt; 0.991). The limits of detection (LOD) were in the range of 0.01–1.29 μg/kg, while the limits of quantification (LOQ) were in the range of 0.02–4.31 μg/kg. The recoveries were observed to be in the range of 60.6–117.7 %, with relative standard deviations (RSDs) of ≤20.6 %. The method is straightforward and highly sensitive, and it satisfies the maximum limits set by the relevant standards of Korea, Canada, the USA, and the EU. It is well-suited for the rapid screening, qualitative, and quantitative analyses of metomidate, acetanilide, dl-methylephedrine, and other substances in foods of animal origin, providing technical assistance for cross-border food safety and testing.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465726"},"PeriodicalIF":3.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging applications of quantitative supercritical fluid chromatography-tandem mass spectrometry for chiral bioanalysis","authors":"Ines C. Santos,&nbsp;Zhihui Zhang,&nbsp;Lina Luo,&nbsp;Brian Melo,&nbsp;Y-J Xue,&nbsp;Jim X. Shen","doi":"10.1016/j.chroma.2025.465727","DOIUrl":"10.1016/j.chroma.2025.465727","url":null,"abstract":"<div><div>Individual stereoisomers of a chiral drug can possess different pharmacokinetic (PK)/pharmacodynamic (PD) properties, leading to different therapeutic/toxicological effects. Therefore, chiral bioanalytical methods are required for individual stereoisomers to assess their PK properties and potential chiral inversion in vivo. Supercritical fluid chromatography (SFC) has not been a mainstay in bioanalytical labs due to limitations of robustness/reliability of old generations of SFC instrumentation. With the significant advances of newer generation SFC instruments and chiral columns, the time is ripe for implementing this technology in bioanalytical labs, particularly for difficult analysis of chiral separations where traditional normal/reversed phase and polar organic mode chromatography are not adequate or require long run times. In this publication, we used six BMS model chiral compounds to systematically examine the key aspects of SFC-MS/MS for chiral bioanalysis (e.g., chiral columns, organic modifiers/additives). The preliminary method development results showed that SFC can provide superior chiral separations in comparison with LC. Subsequent method qualifications and validation and study sample analysis for four of these chiral compounds showed that the developed chiral SFC methods performed well and met the regulatory requirements for bioanalytical method validation and study sample analysis. Our comprehensive evaluation demonstrated that UHPSFC-MS/MS offered robust/reliable chiral assays, with good sensitivity, peak resolution, and sample throughput and is well suited for chiral separation in regulated bioanalysis.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465727"},"PeriodicalIF":3.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenylboronic acid functionalized magnetic ferroferric oxide nanoparticles and capillary electrophoresis for efficient isolation of flavonoid compounds from leaves of Lonicera japonica Thunb","authors":"Yunfeng Yuan ,&nbsp;Xiaoge Wang ,&nbsp;Jinhua Zhu ,&nbsp;Abdelhadi El Jaouhari ,&nbsp;Xiuhua Liu ,&nbsp;Md． Zaved H． Khan","doi":"10.1016/j.chroma.2025.465729","DOIUrl":"10.1016/j.chroma.2025.465729","url":null,"abstract":"<div><div>Flavonoids are bioactive components in natural products, which possess anti-inflammatory, antibacterial, antioxidant, and cardiovascular protective properties. However, due to the complexity and low content of the components in these samples, developing rapid and sensitive methods for the isolation and extraction of flavonoids still remains a challenge in medical and food science. Herein, a 4-formylphenylboronic acid functionalized magnetic Fe₃O₄ nanomaterial (Fe₃O₄@FPBA) was synthesized and applied as a sorbent of magnetic solid-phase extraction (MSPE) to covalently extract flavonoids from leaves of <em>Lonicera japonica</em> Thunb.. The structure, morphology and magnetic properties of Fe₃O₄@FPBA particles were characterized by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM) technologies. Employing luteoloside, luteolin, lonicerin, hyperoside, quercetin and rutin as model compounds, Fe₃O₄@FPBA as sorbent, a MSPE coupling with capillary electrophoresis (CE) method was developed and optimized to detect the flavonoids. Adsorption kinetics display that the adsorption of flavonoids by Fe₃O₄@FPBA is in line with the Quasi-second-order model, which is controlled by chemisorption mechanism, with the equilibrium adsorption capacity ranging from 3.66 to 6.16 mg/g. The isothermal adsorption model shows that the adsorption is more consistent with Freundlich isotherm equation, and the exponent n is around 1. In addition, the material was applied to the leaves of <em>Lonicera japonica</em> Thunb. extract. Four kinds of flavonoids and three other o-hydroxyl compounds were covalently extracted and magnetically separated. Moreover, the material can still maintain high adsorption properties after recycling 5 times. The material possesses strong magnetism and boric acid ligands, which can realize rapid and high-capacity separation and enrichment of flavonoids in liquid samples. Therefore, the strategy offers an innovative method for the extraction and purification of flavonoids from complex natural plants and also provides a research basis for the discovery of new medicinal compounds based on natural products.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1744 ","pages":"Article 465729"},"PeriodicalIF":3.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automation of a modular method (EN 1528) for analysis of GC-amenable pesticides in food of animal origin","authors":"Anna Buettner ,&nbsp;Julia Polley ,&nbsp;Bjoern Hardebusch ,&nbsp;Karl Speer","doi":"10.1016/j.chroma.2024.465620","DOIUrl":"10.1016/j.chroma.2024.465620","url":null,"abstract":"<div><div>Current manual multi-methods for analysis of pesticides are limited due to their complexity and scope of pesticides, high demand for time and solvent or unsuitability for broad types of food of animal origin. The following research presents a novel automated sample preparation and purification method for various food matrices of animal origin, including milk, raw milk, dairy products, cheese, eggs, fish, fish products, and offal. The Ultra-Turrax® Tube Drive System enables quick fat extraction using a solvent mixture of cyclohexane/ethyl acetate/acetonitrile. From the resulting fat extract one aliquot is taken for clean-up and another aliquot for fat content determination, applicable to products with fat contents ranging from 1% to 30%. The automated clean-up, using gel permeation chromatography and solid-phase extraction (primary secondary amine and C18 cartridge), was successfully validated for more than 40 gas chromatography-amenable pesticides relevant to food of animal origin at a level of 10 µg/kg across each matrix. Additionally, 70% of analytes in milk (28 out of 40), 80% in cheese (34 out of 40), 82% in dairy products (38 out of 40), 82% in eggs (46 out of 56), and 85% in fish and offal (48 out of 56) were validated even at a lower level of 0.5 µg/kg.</div><div>The spectrum of pesticides was expanded by employing a more comprehensive mix containing 196 gas chromatography-amenable analytes, spiked at a level of 10 µg/kg. Compared to the modified EN 1528 method, the quantification of analytes increased by 40%, representing an increase from 90 to 150 analytes out of 196 in each matrix. In conclusion, this automated analytical method can be directly implemented as a routine monitoring system for residual pesticide analysis in various matrices of animal origin, providing a robust tool for ensuring food safety.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1741 ","pages":"Article 465620"},"PeriodicalIF":3.8,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}