Journal of Chromatography B最新文献_第6页

From seed to bioactive: unveiling sprouting-driven changes in chickpeas via targeted metabolomics and in silico modelling 从种子到生物活性：通过有针对性的代谢组学和计算机建模揭示鹰嘴豆发芽驱动的变化

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-02-01 Epub Date: 2025-12-24 DOI: 10.1016/j.jchromb.2025.124904

Merna M. Sakr , Reham S. Ibrahim , Amira M. Beltagy , Reham S. Darwish

Sprouting is a widely practiced food processing method, particularly in Western countries. Chickpeas (Cicer arietinum) have been traditionally used in various cuisines worldwide for centuries. Their versatility and nutritional value make them a staple ingredient in many traditional dishes. This study investigated the impact of sprouting chickpea seeds on their phytochemical profile and anticancer properties. Chickpea seeds were germinated under light and dark conditions for eight days, with samples collected on intervals. Extracts were analyzed using UPLC-MS/MS and tested for cytotoxic activity against two different breast cancer cell lines, namely; MDA-MB-231 (triple-negative breast cancer) and HeLa (uterine cervical cancer). UPLC-MS/MS detected 117 metabolites, primarily flavonoids, phenolic acids, and amino acids. Germination affected phytochemical composition and sprout length, with longer sprouts in darkness. Metabolite abundance peaked on day eight, while cytotoxicity was highest on day six. An OPLS model linked cytotoxic effects on HeLa and MDA-MB-231 cells to key metabolites, identified via coefficient plots. Caffeoylquinic acid, naringenin, and biochanin B were major contributors against MDA-MB-231, while hydroxybenzoic acid hexoside, orobol, and biochanin A hexoside were prominent in HeLa cells. Molecular docking of the top three metabolites with IL2 and ABCB1 genes, revealed as common genes between bioactive compounds' genes and cancer genes, showed hydroxybenzoic acid hexoside and naringenin as top binders, aligning with OPLS findings. Although the results demonstrate promising in vitro anticancer potential, further in vivo and mechanistic studies are required to confirm efficacy, safety, and bioavailability before establishing chickpea sprouts as a functional food or a chemo-preventive agent.

发芽是一种广泛应用的食品加工方法，特别是在西方国家。几个世纪以来，鹰嘴豆（Cicer arietinum）一直被用于世界各地的各种菜肴中。它们的多功能性和营养价值使它们成为许多传统菜肴的主要成分。本研究研究了发芽鹰嘴豆种子对其植物化学特征和抗癌特性的影响。鹰嘴豆种子在光照和黑暗条件下发芽8天，每隔一段时间采集一次样本。采用UPLC-MS/MS对提取物进行分析，并检测其对两种不同乳腺癌细胞系的细胞毒活性，即；MDA-MB-231（三阴性乳腺癌）和HeLa（子宫癌）。UPLC-MS/MS检测到117种代谢物，主要是类黄酮、酚酸和氨基酸。发芽影响植物化学成分和芽长，黑暗中芽长。代谢物丰度在第8天达到峰值，而细胞毒性在第6天达到最高。ops模型将HeLa和MDA-MB-231细胞的细胞毒性作用与关键代谢物联系起来，通过系数图确定。咖啡因酰喹啉酸、柚皮素和生物茶豆素B是抗MDA-MB-231的主要贡献者，而对羟基苯甲酸己糖苷、茴香醇己糖苷和生物茶豆素A己糖苷在HeLa细胞中起主要作用。前3位代谢物与IL2和ABCB1基因（生物活性化合物基因与肿瘤基因之间的共同基因）的分子对接显示，羟基苯甲酸已苷和柚皮素为顶部结合物，与ops结果一致。虽然研究结果显示出了良好的体外抗癌潜力，但在确定鹰嘴豆芽作为功能性食品或化学预防剂之前，还需要进一步的体内和机制研究来确认其有效性、安全性和生物利用度。

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引用次数: 0

Lincomycin residues in liver, bovine muscle tissue, milk, poultry meat, eggs, and honey: Method development and validation 肝脏、牛肌肉组织、牛奶、禽肉、鸡蛋和蜂蜜中林可霉素残留：方法开发和验证。

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-02-01 Epub Date: 2025-12-19 DOI: 10.1016/j.jchromb.2025.124902

Omar Khaled , Lamia Ryad , Mostafa Nagi , Fawzy Eissa

This study presents and validates a simple, sensitive, and specific method for the determination of lincomycin in liver, bovine muscle tissue, milk, poultry meat, eggs, and honey. The method employs a liquid-liquid extraction sample preparation technique, eliminating the need for solid-phase extraction cartridges. This approach offers a cost-effective and rapid alternative, which is essential for routine analysis. The validation procedure encompassed selectivity, linearity, limit of detection, limit of quantification, recovery, repeatability, reproducibility, and decision limit. The method demonstrated excellent linearity with correlation coefficients ranging from 0.9977 to 0.9999 across all matrices. Limit of detection values ranged from 0.04 to 2 μg/kg, while limit of quantification values ranged from 0.13 to 6.6 μg/kg. Recovery rates were between 70 % and 124 % and decision limits ranged from 0.15 to 543 μg/kg. Out of the 180 real samples analyzed, 31 were detected as positive. Continuous monitoring studies should be conducted regularly to determine the presence of veterinary drug residues in food of animal origin, poultry meat, and honey. These studies will help identify the sources of these residues and facilitate the implementation of secure preventive and remedial strategies.

本研究提出并验证了一种简单、灵敏、特异的方法，用于测定肝脏、牛肌肉组织、牛奶、禽肉、鸡蛋和蜂蜜中林可霉素的含量。该方法采用液-液萃取样品制备技术，不需要固相萃取筒。这种方法提供了一种具有成本效益和快速的替代方法，对于常规分析是必不可少的。验证程序包括选择性、线性、检测限、定量限、回收率、可重复性、再现性和决策限。结果表明，该方法线性良好，相关系数在0.9977 ~ 0.9999之间。检出限为0.04 ~ 2 μg/kg，定量限为0.13 ~ 6.6 μg/kg。回收率为70% ~ 124%，决策限为0.15 ~ 543 μg/kg。在分析的180个真实样本中，有31个被检测为阳性。应定期进行持续监测研究，以确定动物源性食品、禽肉和蜂蜜中兽药残留的存在。这些研究将有助于查明这些残留物的来源，并促进执行可靠的预防和补救战略。

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引用次数: 0

Assessment of serum concentrations of α-pinene, β-pinene, and limonene in the U.S. population from the 2013–2014 National Health and nutrition examination survey 2013-2014年国家健康与营养调查中美国人群血清α-蒎烯、β-蒎烯和柠檬烯浓度的评估

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-02-01 Epub Date: 2025-11-30 DOI: 10.1016/j.jchromb.2025.124873

Lalith Silva, Cody Newman, Deepak Bhandari, Wanzhe Zhu, Benjamin Blount

Terpenes are a class of ubiquitous chemicals with many potential health benefits and harms. Human exposure to terpenes can occur through pathways such as cleaning products, fragrances, diet, or drug-use. α-pinene, β-pinene, and limonene are known to be present in trees, citrus fruits, herbs, marijuana, and cigarette smoke. With little known about human exposure to these compounds, it was important to characterize the baseline level of exposure for the general U.S. population. Using results for 2213 samples for participants 6–80 years with surplus serum available from NHANES 2013–2014, obtained with previously published quantitation methods, we compared serum terpene concentrations in demographic groups and identified likely exposure routes. Multiple linear regression found smoking 10–19 cigarettes per day (CPD) was associated with 35.8 % higher serum limonene and smoking >19 CPD was associated with 44.9 % higher serum limonene compared with non-smokers with no secondhand smoke exposure (p = 0.0002). In addition, we found that recent consumption of citrus-rich foods (rates per 1 kg consumed) was associated with significantly higher serum α-pinene (32.8 %, p = 0.0042) and limonene (82.1 % p = 0.0002). Finally, we observed significant associations between serum terpenes and demographic groups. Females had significantly lower serum limonene (p = 0.0243) compared to males. Hispanic and non-Hispanic Asian people had significantly higher serum β-pinene (p < 0.05) and limonene (p < 0.05) compared to non-Hispanic White people. This work characterizes the baseline of terpene exposure in the general U.S. population.

萜烯是一类普遍存在的化学物质，对健康有许多潜在的益处和危害。人类可以通过清洁产品、香水、饮食或吸毒等途径接触到萜烯。α-蒎烯、β-蒎烯和柠檬烯已知存在于树木、柑橘类水果、草药、大麻和香烟烟雾中。由于对人类暴露于这些化合物的情况知之甚少，因此确定美国普通人群暴露的基线水平是很重要的。使用NHANES 2013-2014中6-80岁参与者的2213份样本的结果，通过先前发表的定量方法获得剩余血清，我们比较了人口统计学组的血清萜烯浓度，并确定了可能的暴露途径。多元线性回归发现，与没有二手烟暴露的非吸烟者相比，每天吸烟10-19支（CPD）与血清柠檬烯升高35.8%相关，吸烟bbb19支（CPD）与血清柠檬烯升高44.9%相关（p = 0.0002）。此外，我们发现，最近食用富含柑橘类的食物（每1公斤食用的比例）与血清α-蒎烯（32.8%,p = 0.0042）和柠檬烯（82.1%,p = 0.0002）显著升高相关。最后，我们观察到血清萜烯与人口统计学群体之间的显著关联。女性血清柠檬烯明显低于男性（p = 0.0243）。西班牙裔和非西班牙裔亚洲人血清β-蒎烯显著升高(p

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引用次数: 0

A new organic hydrophilic monolith stationary phase for HILIC - ELSD topiramate quantification and separation with its main polar impurities in infusion solutions 一种新的有机亲水整体固定相，用于HILIC - ELSD定量分离输液中托吡酯及其主要极性杂质

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-02-01 Epub Date: 2025-12-17 DOI: 10.1016/j.jchromb.2025.124901

Claire Andre , Yves Claude Guillaume

In this study, a hydrophilic monolith doped with fumed silica nanoparticles was in-situ prepared in a liquid chromatography column. It was clearly demonstrated the addition of silica nanoparticles in the polymerization mixture improved the formation of mesopores in the low size domain (5.2 nm) and thus the specific surface area and hydrophilicity of the monolith. Stability studies showed this monolith was not affected after slightly swelling or shrinking under different solvent conditions. The potential of this new column was exemplified by its capability in hydrophilic interaction liquid chromatography conditions (1) to separate five standard compounds (nucleotides) and (2) to monitor the main degradation products of topiramate an anticonvulsant molecule used primarily to treat epilepsy. For this second separation, the optimum HPLC gradient conditions were determined with only 18 experiments using a computer assisted design of experiments - simplex algorithm software developed in our laboratory. This column was then used successfully for the quantification of the different doses (in mg) of topiramate in a meglumin pharmaceutical formulation. This novel HPLC analytical shows promise as a stability-indicating assay and may be suitable for the quality control of topiramate in pharmaceutical préparations.

在本研究中，在液相色谱柱中原位制备了掺杂气相二氧化硅纳米颗粒的亲水单体。结果清楚地表明，在聚合混合物中加入二氧化硅纳米颗粒可以改善低尺寸域（5.2 nm）中孔的形成，从而提高整体的比表面积和亲水性。稳定性研究表明，在不同的溶剂条件下，该单体在轻微膨胀或收缩后不受影响。这种新色谱柱的潜力体现在它在亲水相互作用液相色谱条件下的能力(1)分离五种标准化合物（核苷酸）和(2)监测托吡酯的主要降解产物，托吡酯是一种主要用于治疗癫痫的抗痉挛分子。对于第二次分离，使用本实验室开发的计算机辅助实验设计-单纯形算法软件，仅用18次实验就确定了最佳的HPLC梯度条件。该色谱柱成功地用于定量不同剂量（mg）的托吡酯在甲氨基明药物制剂。这种新的高效液相色谱分析方法有望作为一种稳定性指示分析方法，适用于制药行业中托吡酯的质量控制。

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引用次数: 0

Pesticide residues in aquaculture salmon filet and feed: Quantitative and qualitative screening profiles of legacy and current-use pesticides using GC-EI-MS/MS and LC-ESI-IMS-QToF 水产养殖鲑鱼鱼片和饲料中的农药残留：使用GC-EI-MS/MS和LC-ESI-IMS-QToF对遗留和当前使用的农药进行定量和定性筛选。

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-26 DOI: 10.1016/j.jchromb.2025.124864

Carey E. Donald, Annette Bernhard, Marianne F. Hauglid , Annie Fürstenberg, Marc H.G. Berntssen, Anne-Katrine Lundebye, Veronika Sele

In recent decades, the ingredients used in aquaculture feeds have changed from marine-based to more plant-based. As a result, the content of legacy, organochlorine pesticides has decreased, but the potential increase of current use, land-based pesticides has not been documented. In this work, two analytical techniques were developed and applied to characterize the pesticide profile in current Norwegian salmon aquaculture after the shift to more plant-based feed ingredients. Samples of salmon filet, feed, and feed ingredients were analyzed using two different chromatographic methods, a quantitative gas-chromatography-triple quadrupole mass spectrometry method (43 analytes) and a broader, presence/absence screen using liquid chromatography-high resolution mass spectrometry (215 analytes). Among all samples and between both methods, a total of 43 different pesticide analytes were identified. Results show that the major pesticides present in the filet of farm-raised salmon are the legacy, organochlorine pesticides as contaminants. Conversely the feed is dominated by current-use, organophosphate and pyrethroid pesticides. There were no exceedances of the few established maximum residue limits for contaminants in fish and feed. No pesticides in salmon filet exceeded the default limit of 10 μg/kg, while there were several observations in feed above this level. These results suggest low risk in terms of food safety, however, issues related to animal health cannot be ruled out. The two chromatography methods are compared, and they complement each other by giving an overview of pesticides with both breadth and depth.

近几十年来，水产养殖饲料中使用的成分已经从以海洋为基础转变为以植物为基础。因此，遗留有机氯农药的含量有所下降，但目前使用的陆基农药的潜在增加尚未得到记录。在这项工作中，开发了两种分析技术，并应用于在转向更多植物性饲料成分后，目前挪威鲑鱼水产养殖中的农药特征。使用两种不同的色谱方法分析鲑鱼片、饲料和饲料成分样品，一种是定量气相色谱-三重四极杆质谱法（43种分析物），另一种是更广泛的存在/不存在屏幕，使用液相色谱-高分辨率质谱法（215种分析物）。在所有样品中以及两种方法之间，共鉴定出43种不同的农药分析物。结果表明，养殖鲑鱼鱼片中的主要农药是遗留的有机氯农药。相反，饲料主要是目前使用的有机磷和拟除虫菊酯农药。鱼类和饲料中没有超过规定的几项污染物最大残留限量。鲑鱼鱼片中农药残留量未超过10 μg/kg的默认限值，饲料中农药残留量超过10 μg/kg的情况较多。这些结果表明，就食品安全而言，风险较低，但不能排除与动物健康有关的问题。这两种色谱方法进行了比较，它们通过对农药的广度和深度进行概述来相互补充。

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引用次数: 0

From chemical profiling to bioactivity: Integrating spectrum-effect relationship and activity validation to discover anti-inflammatory markers in Wuwei Qingzhuo Pill 从化学分析到生物活性：整合光谱效应关系和活性验证发现武味清浊丸的抗炎标志物。

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-29 DOI: 10.1016/j.jchromb.2025.124876

Tiantian Shi , Wenhua Yang , Lizhu Yin , Xuena Zhang , Yuewu Wang , Liang Bao , Ren Bu , Jingkun Lu , Tuya Bai , Peifeng Xue , Xin Dong

Efficacy-oriented quality control represents a persistent challenge in traditional medicine due to complex multi-component compositions and the inadequacy of single-compound quantification methods. This study establishes an integrated analytical paradigm combining in vivo pharmacological validation with comprehensive chemical profiling, using the traditional Mongolian formulation Wuwei Qingzhuo Pill (WQP) as an investigative model. We initially demonstrated WQP's significant anti-arthritic efficacy in a collagen-induced arthritis (CIA) rat model, observing marked reduction in joint swelling, arthritis scores, and bone destruction. Subsequent chemical characterization established validated HPLC fingerprints across 19 production batches, revealing consistent quality patterns. Through innovative spectrum-effect relationship analysis integrating gray relational analysis (GRA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we successfully correlated chromatographic features with anti-inflammatory potency, identifying gallic acid, punicalagin, and ellagic acid as critical quality markers. The bioactivity of these markers was further confirmed in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, which significantly inhibited TNF-α and IL-1β release. This research presents the first comprehensive application of HPLC fingerprinting combined with spectrum-effect relationship analysis for quality assessment of WQP, offering a robust methodology that transcends traditional quality control approaches. The identified quality markers provide a scientific foundation for efficacy-based quality control and standardization of WQP, with potential implications for quality evaluation of other traditional medicines.

由于复杂的多组分组成和单一化合物定量方法的不足，以疗效为导向的质量控制在传统医学中是一个持续的挑战。本研究以蒙方乌味清浊丸为研究模型，建立了体内药理验证与综合化学分析相结合的综合分析范式。我们最初在胶原诱导关节炎（CIA）大鼠模型中证明了WQP的显著抗关节炎功效，观察到关节肿胀、关节炎评分和骨破坏明显减少。随后的化学表征在19个生产批次中建立了有效的HPLC指纹图谱，揭示了一致的质量模式。通过结合灰色关联分析（GRA）和正交偏最小二乘法判别分析（OPLS-DA）的创新的光谱效应关系分析，我们成功地将色谱特征与抗炎效力关联起来，确定没食子酸、果酸苷和鞣花酸是关键的质量标记。在脂多糖（LPS）刺激的RAW264.7巨噬细胞中进一步证实了这些标记物的生物活性，显著抑制TNF-α和IL-1β的释放。本研究首次将高效液相色谱指纹图谱与光谱效应关系分析相结合，全面应用于WQP的质量评价，提供了一种超越传统质量控制方法的可靠方法。所鉴定的质量标记为WQP基于疗效的质量控制和标准化提供了科学依据，并对其他传统药物的质量评价具有潜在的指导意义。

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引用次数: 0

Development and validation of a UPLC-MS/MS assay for serum choline and phosphocholine with a pilot application to obesity 开发和验证UPLC-MS/MS测定血清胆碱和磷脂胆碱的方法，并试点应用于肥胖。

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-30 DOI: 10.1016/j.jchromb.2025.124870

Yingting Wu , Jiaqi Xin , Haiyan Cao , Yongsheng Zhao , Mengxi Jiang

The rising global burden of obesity underscores the need to better characterize metabolic alterations associated with this condition. Choline (Cho) and its derivative phosphocholine (ChoP) are key intermediates in phospholipid metabolism, yet their relationships with obesity remain unclear. In this study, we established and validated a UPLC-MS/MS method for quantifying serum Cho and ChoP, and investigated their associations with obesity and fasting glucose in a pilot human cohort. Serum samples from 62 participants (obese vs. nonobese) were analyzed. The primary analysis compared metabolite levels between groups, and key secondary analyses included Pearson correlations with fasting glucose and receiver operating characteristic (ROC)-based discrimination; two-sample Mendelian randomization (MR) was conducted using external GWAS summary statistics. Obese individuals had lower ChoP levels and ChoP/Cho ratios. ChoP and the ChoP/Cho ratio were inversely associated, and Cho was positively associated, with fasting glucose. ChoP showed modest discriminative ability for obesity, and MR suggested that higher ChoP levels may be related to a lower risk of obesity. Overall, we validated a UPLC-MS/MS assay for serum Cho and ChoP, and our findings indicate that ChoP and the ChoP/Cho ratio are linked to obesity and glucose status.

全球肥胖负担的增加强调了更好地描述与肥胖相关的代谢变化的必要性。胆碱（Cho）及其衍生物磷脂胆碱（ChoP）是磷脂代谢的关键中间体，但它们与肥胖的关系尚不清楚。在这项研究中，我们建立并验证了一种UPLC-MS/MS定量血清Cho和ChoP的方法，并研究了它们与肥胖和空腹血糖的关系。分析了62名参与者（肥胖与非肥胖）的血清样本。主要分析比较各组之间的代谢物水平，关键的次要分析包括与空腹血糖的Pearson相关性和基于受试者工作特征（ROC）的区分；采用外部GWAS汇总统计进行双样本孟德尔随机化（MR）。肥胖个体的ChoP水平和ChoP/Cho比值较低。ChoP和ChoP/Cho比值与空腹血糖呈负相关，Cho呈正相关。ChoP对肥胖表现出适度的区分能力，MR表明较高的ChoP水平可能与较低的肥胖风险有关。总之，我们验证了血清Cho和ChoP的UPLC-MS/MS分析，我们的研究结果表明，ChoP和ChoP/Cho比值与肥胖和血糖状态有关。

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引用次数: 0

A method for simultaneous determination of triiodothyronine and thyroxine in fingernail clippings by LC/ESI-MS/MS hplc /ESI-MS/MS同时测定指甲屑中三碘甲状腺原氨酸和甲状腺素的方法

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-24 DOI: 10.1016/j.jchromb.2025.124869

Kenjiro Shibata , Sakurako Tanaka , Shuhei Baba , Arina Miyoshi , Norio Wada , Tatsuya Higashi

A fingernail clipping is a promising specimen for evaluating the medium-to-long term status of low-molecular weight hormones. However, the nail concentrations of thyroid hormones and their clinical significance remain poorly understood. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) method was developed and validated for simultaneously quantifying triiodothyronine (T₃) and thyroxine (T₄) in the thumbnail clippings. The nail T₃ and T₄ were extracted with 50 % ethanol and purified using a Strata-X cartridge, then injected into the LC/ESI-MS/MS. ¹³C₆-T₄ was used as the internal standard for both T₃ and T₄. Validation results demonstrated that the method was precise (relative standard deviations in five repetitive measurements were ≤ 4.5 %) and accurate (analytical recovery rates were 98.3–103.3 %). The lower limits of quantification were 0.20 ng/g for both thyroid hormones. By using this method, we found that the nail concentrations of T₃ (0.74 ± 0.26 ng/g, mean ± standard deviation) were at the same level as those of T₄ (0.90 ± 0.27 ng/g) in euthyroid subjects (n = 40). This result might be caused due to the greater free and albumin-bound fractions of circulating T₃ compared to T₄. The patients with Graves' disease showed significantly higher nail T₃ and T₄ concentrations than the euthyroid subjects. The patients with hypothyroidism due to Hashimoto's thyroiditis tended to have lower nail T₃ and T₄ concentrations compared to the euthyroid subjects. Thus, the developed method would be useful for evaluating the medium-to-long term status of thyroid hormone production and has sufficient clinical applicability.

指甲修剪是评估低分子量激素中长期状态的有希望的标本。然而，指甲中甲状腺激素的浓度及其临床意义仍然知之甚少。本研究建立了一种液相色谱/电喷雾电离-串联质谱（LC/ESI-MS/MS）方法，用于同时测定缩略指甲剪影中的三碘甲状腺原氨酸（T3）和甲状腺素（T4）。采用50%乙醇提取T3、T4，经Strata-X试剂盒纯化后，进样于LC/ESI-MS/MS中。T3和T4均采用13C6-T4作为内标。验证结果表明，该方法精密度高（5次重复测定的相对标准偏差≤4.5%），准确度高（分析回收率为98.3 ~ 103.3%）。两种甲状腺激素的定量下限均为0.20 ng/g。结果显示，40例甲状腺功能正常的患者T3指甲浓度（0.74±0.26 ng/g，平均值±标准差）与T4指甲浓度（0.90±0.27 ng/g）基本一致。这一结果可能是由于与T4相比，循环T3的游离和白蛋白结合部分更大。Graves病患者指甲T3、T4浓度明显高于甲状腺功能正常者。桥本甲状腺炎所致甲状腺功能减退患者甲3、T4浓度较甲状腺功能正常者低。因此，该方法可用于评估甲状腺激素产生的中长期状态，具有足够的临床适用性。

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引用次数: 0

Per- and polyfluoroalkyl substances (PFAS) in marine sediments from a Norwegian fjord near an airport: Historical records, distribution, and controlling factors 机场附近挪威峡湾海洋沉积物中的全氟和多氟烷基物质（PFAS）：历史记录、分布和控制因素

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-26 DOI: 10.1016/j.jchromb.2025.124868

Stepan Boitsov, Jon Albretsen, Hilde Elise Heldal, Stig Valdersnes, Bjørn Einar Grøsvik, Bashir Abdulkader, Aasim Ali

Airports have been identified as one of numerous sources of extremely persistent, toxic and bioaccumulative per- and polyfluoroalkyl substances (PFAS), as part of aqueous film-forming foams (AFFF) during fire drills. The historically strongly contaminated terrestrial environment around Flesland airport in Bergen, Norway, raised concern for potential contamination in the surrounding fjords. To investigate this, surface sediments and sediment cores were collected from several locations adjacent to the airport area. One of the cores was dated by ²¹⁰Pb. Two analytical methods, a target method for 24 specific PFAS compounds in marine sediments, and a total oxidizable precursor (TOP) assay method, were established, validated and applied to the samples. The results were combined with previously collected data on surface sediments from fjords and open sea areas in Western Norway. While TOP assay results for sediments and pore water indicated a significant PFAS precursor load at locations close to the airport, and sediment cores showed a continuous increase in PFAS levels (since at least 1980s in the dated core), relatively low levels of PFAS were found in marine sediments off the airport area (1.1 μg/kg dry weight or less for the sum of nine most abundant PFAS in surface sediments). The results agree with earlier data on PFAS background levels in marine sediments from unaffected areas and imply a dispersion of the contamination from the airport elsewhere. Sea current modeling and grain size determination suggest local hydrodynamical and sedimentological conditions as the main factors of influence for the contamination status in the area.

机场已被确定为消防演习期间作为水成膜泡沫（AFFF）的一部分的极持久、有毒和生物蓄积性全氟烷基和多氟烷基物质（PFAS）的众多来源之一。挪威卑尔根的弗莱斯兰机场周围的陆地环境历来受到严重污染，这引起了人们对周围峡湾潜在污染的关注。为了调查这一点，从机场附近的几个地点收集了地表沉积物和沉积物岩心。其中一个岩心的年代为210Pb。建立了海洋沉积物中24种特定PFAS化合物的靶法和总氧化前体（TOP）法两种分析方法，并对其进行了验证和应用。研究结果与之前收集的挪威西部峡湾和公海表面沉积物数据相结合。虽然沉积物和孔隙水的TOP分析结果表明，靠近机场的位置有显著的PFAS前体负荷，沉积物岩心显示PFAS水平持续增加（至少从20世纪80年代开始），但在机场区域附近的海洋沉积物中发现的PFAS水平相对较低（地表沉积物中9种最丰富的PFAS的总和为1.1 μg/kg干重或更低）。这一结果与早期关于未受影响地区海洋沉积物中PFAS背景水平的数据一致，表明污染从其他地方的机场扩散开来。海流模拟和粒度测定表明，当地的水动力和沉积条件是影响该地区污染状况的主要因素。

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引用次数: 0

Comparative evaluation between Geenius™ dual-path migration immunochromatography platform and Western blot HIV-1 for confirmatory assays in the Brazilian population Geenius™双路迁移免疫层析平台和Western blot HIV-1在巴西人群验证性检测中的比较评价

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B

Pub Date : 2026-01-15 Epub Date: 2025-11-29 DOI: 10.1016/j.jchromb.2025.124885

Rubia Heloisa Vanderlinde, Graziele Cristina Ferreira, Jussimara Monteiro Nurmberger, Daniela Calciolari Marques, Flavia Maria Correa Cristaldi, Renata Chiavarini, Debora Ribeiro Ramadan, Sergio Tufik, Carolina Xavier Lima Piccinini, Mariana do Nascimento Viana

Human immunodeficiency virus (HIV) diagnosis requires both screening and confirmatory assays to accurately differentiate antigen and antibody reactivity. The Western blot (WB) method has long been considered the gold standard for confirming HIV infection. However, due to its labor-intensive workflow and technical complexity, the WB has become challenging for many clinical laboratories. Therefore, newer diagnostic techniques, such as the Dual-Path Platform (DPP) lateral flow immunochromatography assays, have been developed and approved by regulatory agencies. Automated interpretation of DPP enhances analytical sensitivity by minimizing subjectivity and allowing detection of faint bands, reducing the probability of false-negative results. This study compared the performance of the WB and DPP as confirmatory methods following chemiluminescent immunoassay (CLIA) screening. All samples were previously screened using CLIA and subsequently analyzed by both WB and DPP assays. Samples yielding borderline CLIA results underwent additional testing by RT-qPCR. The DPP platform demonstrated high concordance with the WB assay, achieving an agreement rate of 97.2 % and a kappa index of 93.9 %, indicating excellent predictive performance. Borderline results were confirmed as reactive by both DPP and RT-qPCR but remained indeterminate by WB. Although complete agreement between both confirmatory methods was not achieved, no clinically significant discrepancies were observed, suggesting that such variations did not impact patient outcomes. Despite the WB remaining as a reference confirmatory method for HIV diagnosis, the DPP method offers substantial advantages, including shorter turnaround time, greater operational simplicity, and automated digital documentation, making it a practical alternative for routine use in high-throughput or resource-limited laboratory settings.

人类免疫缺陷病毒（HIV）的诊断需要筛查和确认试验来准确区分抗原和抗体的反应性。长期以来，Western blot （WB）方法一直被认为是确认HIV感染的金标准。然而，由于其劳动密集型工作流程和技术复杂性，WB已成为许多临床实验室的挑战。因此，新的诊断技术，如双路径平台（DPP）横向流动免疫层析分析，已经开发并得到监管机构的批准。DPP的自动解释通过最大限度地减少主观性和允许检测微弱波段来提高分析灵敏度，减少假阴性结果的可能性。本研究比较了WB和DPP作为化学发光免疫测定（CLIA）筛选后的验证性方法的性能。所有样品先前使用CLIA筛选，随后使用WB和DPP分析。获得CLIA临界结果的样品通过RT-qPCR进行了额外的检测。DPP平台与WB法具有较高的一致性，一致性率为97.2%，kappa指数为93.9%，具有良好的预测性能。DPP和RT-qPCR均证实边缘性结果具有反应性，但WB仍不确定。虽然两种验证性方法之间没有完全一致，但没有观察到临床上显著的差异，这表明这些差异不会影响患者的预后。尽管WB仍然是HIV诊断的参考验证方法，但DPP方法具有实质性优势，包括周转时间更短，操作更简单，自动化数字文档，使其成为高通量或资源有限的实验室环境中常规使用的实用替代方案。

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引用次数: 0