Pub Date : 2023-05-27DOI: 10.14232/abs.2022.2.170-179
Belay Teweldemedhin Keleta, Z. Békefi, József László Bakos, D. Örsi, L. Szalay
Frost hardiness of flower buds of twenty almond genotypes was investigated in five dormancy periods by determining LT50 values after artificial freezing tests. The main aim of our work was modelling the changing of frost hardiness of the observed accessions during dormancy and assessing the potential best frost tolerance of them. The effect of genotype and year had significant impact on frost hardiness of flower buds. The potential frost hardiness of accessions has been characterised by LT50 values of flower buds averaged of the bests of the four years. ‘Sóskút 96/5’ was the most sensitive with -17.16 °C, and ‘Tétényi keményhéjú’ was the most frost hardy with -21.08 °C in averaged of years, but both showed lower and higher frost tolerance as well in different years. Flower buds were most frost-tolerant in December and January but did not achieve the same frost resistance every year. From this, we conclude that temperature plays an important role in the hardening process of them. From the aspect of safe yield, frost hardiness of flower buds is an important trait of cultivars, because Hungary is situated at the northern part of economical almond growing area. Our work contributes to facilitating practical considerations in orchard planning.
{"title":"Frost hardiness of almond flower buds during dormancy","authors":"Belay Teweldemedhin Keleta, Z. Békefi, József László Bakos, D. Örsi, L. Szalay","doi":"10.14232/abs.2022.2.170-179","DOIUrl":"https://doi.org/10.14232/abs.2022.2.170-179","url":null,"abstract":"Frost hardiness of flower buds of twenty almond genotypes was investigated in five dormancy periods by determining LT50 values after artificial freezing tests. The main aim of our work was modelling the changing of frost hardiness of the observed accessions during dormancy and assessing the potential best frost tolerance of them. The effect of genotype and year had significant impact on frost hardiness of flower buds. The potential frost hardiness of accessions has been characterised by LT50 values of flower buds averaged of the bests of the four years. ‘Sóskút 96/5’ was the most sensitive with -17.16 °C, and ‘Tétényi keményhéjú’ was the most frost hardy with -21.08 °C in averaged of years, but both showed lower and higher frost tolerance as well in different years. Flower buds were most frost-tolerant in December and January but did not achieve the same frost resistance every year. From this, we conclude that temperature plays an important role in the hardening process of them. From the aspect of safe yield, frost hardiness of flower buds is an important trait of cultivars, because Hungary is situated at the northern part of economical almond growing area. Our work contributes to facilitating practical considerations in orchard planning.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43895217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.14232/abs.2022.2.132-138
D. Kumar, Ahmad Ali
Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.
{"title":"Effect of different artificial sweeteners on protein glycation","authors":"D. Kumar, Ahmad Ali","doi":"10.14232/abs.2022.2.132-138","DOIUrl":"https://doi.org/10.14232/abs.2022.2.132-138","url":null,"abstract":"Glycation refers to the non-enzymatic molecular interaction between carbonyl group of sugars and amino groups of macromolecules viz. proteins, DNA, and lipids leading to the generation of Schiff’s base, Amadori products, and finally converted to deleterious advanced glycation end-products (AGEs). Several diseases such as neurodegenerative or mental disorders, cardiovascular complications, as well as diabetes, and its related complications show glycated product involvement. Hyperglycemia and diabetes are the main diseases in which AGEs formation and its accumulation are enhanced and cause secondary complications. This study was performed to investigate the antiglycation and anti-aggregation potential of Food and Drug Administration-approved artificial sweeteners. The in vitro glycation system (BSA and glucose) was incubated along with artificial sweeteners viz acesulfame potassium, saccharin sodium, sucralose, aspartame, and neotame for 35 days at 37 °C. The conventional analytical methods such as browning, NBT assay, DNPH assay, and assessment of fluorescent AGEs were carried out spectroscopically to check the amount of glycation products. The presence of the mentioned artificial sweeteners in the glycation system showed inhibition of carbonyl content, total AGEs generation, and aggregation of β-amyloid structures. On day 35, acesulfame potassium reduced carbonyl content by 62.63 ± 0.91%, total AGEs generation by 49.39 ± 0.82%, and β-amyloid aggregation observed by Thioflavin-T assay by 43.45 ± 1.14%. The tested artificial sweeteners exhibited potential antiglycation and anti-aggregation activity in vitro in protein, BSA. They may be used as a therapeutic agent for the management of diabetes and its complications.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49380619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-06DOI: 10.14232/abs.2022.1.17-22
Mariam Jlassi, B. Jemmali, Hatem Ouled Ahmed, F. Lasfer, Aberrahmene Ben Gara
Diversity of Arabian stallions distributed throughout the territory of Tunisia has been evaluated. Seventeen microsatellite markers were used for the determination of genetic variation. A total of 95 alleles were detected in the 50 stallions studied. The number of alleles per locus ranged from 2 to 9 with an average of 5.6. The observed mean number of genetically diverse alleles (Na) was 5.6 (0.44). The mean number of expected alleles (Ne) was 3.42 (0.28). The average expected and observed heterozygosity was approximately 0.675 (0.026) and 0.593 (0.044), respectively. Principal component analysis showed the presence of 2 subpopulations in the studied sample set. These findings demonstrate the potential of microsatellites as a tool for designing and controlling animal breeds. Results show that the population under study has sufficient levels of genetic variations, which can be used as a foundation for developing plans for species conservation and long-term sustainability.
{"title":"Genotypic study of Tunisian Arabian stallions","authors":"Mariam Jlassi, B. Jemmali, Hatem Ouled Ahmed, F. Lasfer, Aberrahmene Ben Gara","doi":"10.14232/abs.2022.1.17-22","DOIUrl":"https://doi.org/10.14232/abs.2022.1.17-22","url":null,"abstract":"Diversity of Arabian stallions distributed throughout the territory of Tunisia has been evaluated. Seventeen microsatellite markers were used for the determination of genetic variation. A total of 95 alleles were detected in the 50 stallions studied. The number of alleles per locus ranged from 2 to 9 with an average of 5.6. The observed mean number of genetically diverse alleles (Na) was 5.6 (0.44). The mean number of expected alleles (Ne) was 3.42 (0.28). The average expected and observed heterozygosity was approximately 0.675 (0.026) and 0.593 (0.044), respectively. Principal component analysis showed the presence of 2 subpopulations in the studied sample set. These findings demonstrate the potential of microsatellites as a tool for designing and controlling animal breeds. Results show that the population under study has sufficient levels of genetic variations, which can be used as a foundation for developing plans for species conservation and long-term sustainability.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46749090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.97-97
Mycheli.P. da Cruz, Luca Larentis, Edgar de S. Vismara, L. Vismara, Patricia F. de Freitas, S. Mazaro
Erratum
勘误表
{"title":"Erratum: Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe diffusa and embryotoxicity assessment in a chicken embryo model","authors":"Mycheli.P. da Cruz, Luca Larentis, Edgar de S. Vismara, L. Vismara, Patricia F. de Freitas, S. Mazaro","doi":"10.14232/abs.2022.1.97-97","DOIUrl":"https://doi.org/10.14232/abs.2022.1.97-97","url":null,"abstract":"<jats:p>Erratum</jats:p>","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44863864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asmaul Husna, Md. Rashedul Kabir Mondol, Md. Rabiul Hasan, M. Y. Hossain
Black pomfret (Parastromateus niger) is one of the major commercial species of pomfret fishery in Bangladesh. This study illustrates the population structure (Length Frequency Distribution, LFD), relationship between length and weight (LWR), relationships between length and length (LLRs), Fulton’s condition factor (KF) and relative weight (WR) of P. niger in the Bay of Bengal (BoB). A total of 225 P. niger were collected from the four locations during January to December 2020. LFD analysis indicates three length classes 21-27 cm, 30-32 cm and 35-37 cm, respectively. This species showed isometric growth pattern (b = 2.981) that indicates that the size and weight increases proportionally and the surrounding habitat provides favourable environment for the growth. LWR between TL and BW were highly correlated (r² = 0.951). LLRs also showed significant correlation between TL and SL (r² = 0.845) and TL and FL (r² = 0.861). The mean value of KF was found as 1.60 which indicates that the BoB provides healthy environment for this species. The mean value of WR (101.09) indicates that the relationship between prey and predator was in balanced condition. This study suggests optimum catchable length for P. niger at 27 cm. Therefore, these findings could provide important information to design effective conservation and management planning for this species.
{"title":"Estimation of population structure, growth and condition of Parastromateus niger in the Bay of Bengal","authors":"Asmaul Husna, Md. Rashedul Kabir Mondol, Md. Rabiul Hasan, M. Y. Hossain","doi":"10.14232/abs.2022.1.1-6","DOIUrl":"https://doi.org/10.14232/abs.2022.1.1-6","url":null,"abstract":"Black pomfret (Parastromateus niger) is one of the major commercial species of pomfret fishery in Bangladesh. This study illustrates the population structure (Length Frequency Distribution, LFD), relationship between length and weight (LWR), relationships between length and length (LLRs), Fulton’s condition factor (KF) and relative weight (WR) of P. niger in the Bay of Bengal (BoB). A total of 225 P. niger were collected from the four locations during January to December 2020. LFD analysis indicates three length classes 21-27 cm, 30-32 cm and 35-37 cm, respectively. This species showed isometric growth pattern (b = 2.981) that indicates that the size and weight increases proportionally and the surrounding habitat provides favourable environment for the growth. LWR between TL and BW were highly correlated (r² = 0.951). LLRs also showed significant correlation between TL and SL (r² = 0.845) and TL and FL (r² = 0.861). The mean value of KF was found as 1.60 which indicates that the BoB provides healthy environment for this species. The mean value of WR (101.09) indicates that the relationship between prey and predator was in balanced condition. This study suggests optimum catchable length for P. niger at 27 cm. Therefore, these findings could provide important information to design effective conservation and management planning for this species.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46991050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.29-36
Alayasa Nadeim, T. P. Shkurat
Pre-eclampsia (PЕ) is defined as a severe gestational condition that appears after the twentieth weeks of pregnancy, aff ecting 5-8% worldwide. Circulating microRNАs are short, noncoding RNA molecules. The role of miRNAs was studied in many publications related to PЕ; however, the results have been inconsistent due to variety of diagnostic and prognostic values. Therefore, we conducted a mеtа-аnаlysis study tо quantify the general diagnostic effects of circulating miRNAs in the diagnosis of PЕ. We searched chosen databases and systematically collected publications for analysis from January 2017 till June 2021. Following the screening of the literature and the extraction of data. After that, we conducted a quality evaluation using the QUADAS-2 score system. A bivariate-random effect mеtа-analysis model was then used to construct the pooled diagnostic parameters. To identify the causes of heterogeneity, we conduct the threshold effect analysis as well as the subgroup analysis. Fagan`s Nomogram was used to validate the clinical utility. Moreover, sensitivity and specificity analysis were used to evaluate each study’s reliability, and to investigate the publication-bias we conducted the funnel plot asymmetry test. Our mеtа-analysis involved 8 articles, containing in total 704 pregnant women, 354 pre-eclampsia patients and 350 uncomplicated, normal pregnancy. According to the results, the total pooled results of sensitivity, specificity, and DOR were as follows: 0.88 (95% CI: 0.86-0.90), 0.87 (95% CI: 0.85-0.89) and 57.54 (95% CI: 35.24-93.94), respectively. Moreover, subgroup analysis indicated that non plasma samples and non-Asian ethnicity had higher diagnostic value, however we didn’t conduct a subgroup-analysis for the internal references subgroup due to inadequate data. We concluded that the circulating miRNAs could be used as a screening tool for pre-eclampsia diagnosis. Our mеtа-analysis shows that circulating microRNАs serve as PЕ biomarkers because of their high sensitivity and specificity. In addition, further studies using a bigger sample size is needed for better assessment of miRNAs in the diagnosis of pre-eclampsia.
{"title":"Expression of circulating microRNAs as diagnostic markers of prеeclampsia","authors":"Alayasa Nadeim, T. P. Shkurat","doi":"10.14232/abs.2022.1.29-36","DOIUrl":"https://doi.org/10.14232/abs.2022.1.29-36","url":null,"abstract":"Pre-eclampsia (PЕ) is defined as a severe gestational condition that appears after the twentieth weeks of pregnancy, aff ecting 5-8% worldwide. Circulating microRNАs are short, noncoding RNA molecules. The role of miRNAs was studied in many publications related to PЕ; however, the results have been inconsistent due to variety of diagnostic and prognostic values. Therefore, we conducted a mеtа-аnаlysis study tо quantify the general diagnostic effects of circulating miRNAs in the diagnosis of PЕ. We searched chosen databases and systematically collected publications for analysis from January 2017 till June 2021. Following the screening of the literature and the extraction of data. After that, we conducted a quality evaluation using the QUADAS-2 score system. A bivariate-random effect mеtа-analysis model was then used to construct the pooled diagnostic parameters. To identify the causes of heterogeneity, we conduct the threshold effect analysis as well as the subgroup analysis. Fagan`s Nomogram was used to validate the clinical utility. Moreover, sensitivity and specificity analysis were used to evaluate each study’s reliability, and to investigate the publication-bias we conducted the funnel plot asymmetry test. Our mеtа-analysis involved 8 articles, containing in total 704 pregnant women, 354 pre-eclampsia patients and 350 uncomplicated, normal pregnancy. According to the results, the total pooled results of sensitivity, specificity, and DOR were as follows: 0.88 (95% CI: 0.86-0.90), 0.87 (95% CI: 0.85-0.89) and 57.54 (95% CI: 35.24-93.94), respectively. Moreover, subgroup analysis indicated that non plasma samples and non-Asian ethnicity had higher diagnostic value, however we didn’t conduct a subgroup-analysis for the internal references subgroup due to inadequate data. We concluded that the circulating miRNAs could be used as a screening tool for pre-eclampsia diagnosis. Our mеtа-analysis shows that circulating microRNАs serve as PЕ biomarkers because of their high sensitivity and specificity. In addition, further studies using a bigger sample size is needed for better assessment of miRNAs in the diagnosis of pre-eclampsia.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46502934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.63-68
M. Keshavarzi, Z. N. Bokaee, N. Derakhshan
Lolium (Poaceae) is composed of five species in Iran that are of forage and weed importance. Culm and glume anatomy of grasses is inadequately explored. In the present study culm and glume anatomical structures of 17 population of four Lolium species is considered by use of 16 qualitative and quantitative features to distinguish Lolium species in Iran and to determine their diagnostic value. The culm cross-section showed multilayered hypodermis, ribbed or un-ribbed culm cross-sections, cuticles with different thicknesses, and the cortex with different tissues. Vascular bundles are arranged in two to more rings in the culm anatomy. Glume anatomical observations showed differences in girder attachment to vascular bundles, the outline of the cross-section, and the cortex tissue composition. The multi-variate statistical method based on culm and glume anatomical features showed that L. perenne and L. multiflorum are grouped near each other but there is more mixture between L. persicum and L. rigidum. Our findings are in agreement with the grouping pattern achieved by previous researches on leaf anatomy and lemma and palea micro-morphology of the same species. The molecular study by ISSR also provides support for the present study. The culm and glume anatomy of species studied provides valuable data for taxonomic purposes.
{"title":"The culm and glume anatomical study on Lolium (Poaceae) species in Iran","authors":"M. Keshavarzi, Z. N. Bokaee, N. Derakhshan","doi":"10.14232/abs.2022.1.63-68","DOIUrl":"https://doi.org/10.14232/abs.2022.1.63-68","url":null,"abstract":"Lolium (Poaceae) is composed of five species in Iran that are of forage and weed importance. Culm and glume anatomy of grasses is inadequately explored. In the present study culm and glume anatomical structures of 17 population of four Lolium species is considered by use of 16 qualitative and quantitative features to distinguish Lolium species in Iran and to determine their diagnostic value. The culm cross-section showed multilayered hypodermis, ribbed or un-ribbed culm cross-sections, cuticles with different thicknesses, and the cortex with different tissues. Vascular bundles are arranged in two to more rings in the culm anatomy. Glume anatomical observations showed differences in girder attachment to vascular bundles, the outline of the cross-section, and the cortex tissue composition. The multi-variate statistical method based on culm and glume anatomical features showed that L. perenne and L. multiflorum are grouped near each other but there is more mixture between L. persicum and L. rigidum. Our findings are in agreement with the grouping pattern achieved by previous researches on leaf anatomy and lemma and palea micro-morphology of the same species. The molecular study by ISSR also provides support for the present study. The culm and glume anatomy of species studied provides valuable data for taxonomic purposes.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47383928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.7-15
O. Fedota, N. Puzik, I. Skrypkina, V. Babalyan, L. Mitiohlo, S. Ruban, S. Belyaev, O. O. Borshch, O. V. Borshch
The chronic consumption of mycotoxin-contaminated forage tends to the weight loss of young cattle, decreasing the reproductive success of adult animals, milk yield, and tolerance to high temperatures. The aim of this paper was to study the eff ects of the SNP C994G in the CYP3A28 gene on the productive and reproductive characteristics of dairy and beef breeds. Cows were measured by body weight dynamics, exterior, reproduction, milk production traits, bone mineral density, bulls were analyzed estimating their progeny traits. Dairy cows received dietary betaine supplement with the measurement of homocysteine levels. Molecular genetic was performed by PCR-RFLP method. The effects of SNP C994G were shown in relation to the udder size, the constitution and birth weight of Abredin-Angus cows, as well as the fat and protein content of Ukrainian Red-and-White Dairy cows milk. The higher sensitivity of the GG genotype to less homocysteine level after the addition of betaine was shown. Given the identifi ed correspondence, pleiotropic eff ects of CYP3A28 gene can be concluded. A microscopic analysis of forage supported the findings in relation to greater efficiency of the CC genotype under conditions of low mycotoxins contamination, to which CYP3A28 is selective.
{"title":"Single nucleotide polymorphism C994g of the cytochrome P450 gene possess pleiotropic effects in Bos taurus, L.","authors":"O. Fedota, N. Puzik, I. Skrypkina, V. Babalyan, L. Mitiohlo, S. Ruban, S. Belyaev, O. O. Borshch, O. V. Borshch","doi":"10.14232/abs.2022.1.7-15","DOIUrl":"https://doi.org/10.14232/abs.2022.1.7-15","url":null,"abstract":"The chronic consumption of mycotoxin-contaminated forage tends to the weight loss of young cattle, decreasing the reproductive success of adult animals, milk yield, and tolerance to high temperatures. The aim of this paper was to study the eff ects of the SNP C994G in the CYP3A28 gene on the productive and reproductive characteristics of dairy and beef breeds. Cows were measured by body weight dynamics, exterior, reproduction, milk production traits, bone mineral density, bulls were analyzed estimating their progeny traits. Dairy cows received dietary betaine supplement with the measurement of homocysteine levels. Molecular genetic was performed by PCR-RFLP method. The effects of SNP C994G were shown in relation to the udder size, the constitution and birth weight of Abredin-Angus cows, as well as the fat and protein content of Ukrainian Red-and-White Dairy cows milk. The higher sensitivity of the GG genotype to less homocysteine level after the addition of betaine was shown. Given the identifi ed correspondence, pleiotropic eff ects of CYP3A28 gene can be concluded. A microscopic analysis of forage supported the findings in relation to greater efficiency of the CC genotype under conditions of low mycotoxins contamination, to which CYP3A28 is selective.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43483030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.37-46
Bahareh Ghasemi, H. Sabouri, H. H. Moghaddam, A. Biabani, Mohamad Javad Sheikhzadeh
Rice germplasm investigated as completely randomized design under flooding and deficit irrigation conditions. The results of the association analysis indicated that RM29, RM63, and RM53 could be used for rice breeding programs to improve yields under deficit irrigation. The highest accuracy of rice performance prediction was 98.36 for the RFA (RFA) for panicle length, flag leaf length, and width, and the number of primary branches, after that, the MLP algorithm had better prediction power than other algorithms. When a genotypes code was considered as a criterion to classify the genotypes under the drought stress at the reproductive stage, the random forest algorithm (RFA) was the best algorithm based on the predictive accuracy (67.93), kappa value (0.514) and root mean square error (0.293). Based on the artificial intelligence methods, the RFA presented the best results to predict the response of genotypes to deficit irrigation using the microsatellite molecular data.
{"title":"Rice performance prediction to deficit irrigation using microsatellite alleles and artificial intelligence","authors":"Bahareh Ghasemi, H. Sabouri, H. H. Moghaddam, A. Biabani, Mohamad Javad Sheikhzadeh","doi":"10.14232/abs.2022.1.37-46","DOIUrl":"https://doi.org/10.14232/abs.2022.1.37-46","url":null,"abstract":"Rice germplasm investigated as completely randomized design under flooding and deficit irrigation conditions. The results of the association analysis indicated that RM29, RM63, and RM53 could be used for rice breeding programs to improve yields under deficit irrigation. The highest accuracy of rice performance prediction was 98.36 for the RFA (RFA) for panicle length, flag leaf length, and width, and the number of primary branches, after that, the MLP algorithm had better prediction power than other algorithms. When a genotypes code was considered as a criterion to classify the genotypes under the drought stress at the reproductive stage, the random forest algorithm (RFA) was the best algorithm based on the predictive accuracy (67.93), kappa value (0.514) and root mean square error (0.293). Based on the artificial intelligence methods, the RFA presented the best results to predict the response of genotypes to deficit irrigation using the microsatellite molecular data.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44508065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.14232/abs.2022.1.69-73
Oyetunde T. Oyeyemi, Chioma G. Eneh, Ijeoma J. Okeke
Snails control is considered the most effective method in reducing the transmission of fascioliasis. Chemical molluscicides are reported to be toxic and ineffective due to snail resistance, hence, the need for safer and effective molluscicides. The aim of this study was to assess the molluscicidal activities of two medicinal plants against snail host of Fasciola gigantica. The molluscicidal activity of Morinda lucida and Calotropis procera was investigated against Lymnaea natalensis, the intermediate host of F. gigantica. Ethanolic extract of leaves of the tested plants were evaluated for their lethal activity against the embryos within the egg masses and adult L. natalensis. All the two tested plants exerted toxic lethal effect against both the embryos and the adult snails. While M. lucida extract appeared to be more potent at lower concentration on the embryos, the extract of C. procera leaves was significantly more potent than that of M. lucida in adult snails (P<0.05). The LC50 of ethanolic extracts of M. lucida and C. procera was 1.698 mg/ml and 0.500 mg/ml, respectively, at 72 h exposure time of adult L. natalensis. The two tested plants have molluscicidal activities, but these activities could be stage specific at certain concentrations.
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