Pub Date : 2021-01-01DOI: 10.14232/abs.2021.1.35-45
S. Mosaferi, M. Keshavarzi
Aeluropus from Poaceae comprises 5 species in the world and 3 species in Iran. This halophytic perennial is distributed in salty and dry soils of Asia, Europe, and Africa. In addition to being used as fodder, it can stabilize the soil by its rhizome or stolon. These features make Aeluropus a valuable plant. In this study, lemma and palea of 10 populations of Aeluropus were studied micromorphologically by scanning electron microscope (SEM) to determine diagnostic features among species studied. Eight characters as micro-prickle, macro-hair, long cell outline, cork and silica cells, papilla, salt gland, and epicuticular wax were studied. The occurrence of salt glands and silica cells in populations/taxa studied showed the ability of Aeluropus to tolerate harsh habitats. Our result showed the taxonomic value of floret micromorphological features to separate Aeluropus species.
{"title":"Epidermal micromorphology of floret parts in Aeluropus (Poaceae)","authors":"S. Mosaferi, M. Keshavarzi","doi":"10.14232/abs.2021.1.35-45","DOIUrl":"https://doi.org/10.14232/abs.2021.1.35-45","url":null,"abstract":"Aeluropus from Poaceae comprises 5 species in the world and 3 species in Iran. This halophytic perennial is distributed in salty and dry soils of Asia, Europe, and Africa. In addition to being used as fodder, it can stabilize the soil by its rhizome or stolon. These features make Aeluropus a valuable plant. In this study, lemma and palea of 10 populations of Aeluropus were studied micromorphologically by scanning electron microscope (SEM) to determine diagnostic features among species studied. Eight characters as micro-prickle, macro-hair, long cell outline, cork and silica cells, papilla, salt gland, and epicuticular wax were studied. The occurrence of salt glands and silica cells in populations/taxa studied showed the ability of Aeluropus to tolerate harsh habitats. Our result showed the taxonomic value of floret micromorphological features to separate Aeluropus species.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.14232/abs.2021.1.11-16
B. Saleh
Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and Salvia palaestina Benth (Palestinian sage) species using touch-up directed amplification of minisatellite region DNA (TU-DAMD) has been performed in two separated sets; in the first set (set A) the initial annealing temperature was increased from 50 °C to 55 °C, whereas, in the second one (set B), it increased from 55 °C to 60 °C by 0.5 °C/cycle during the first 10 PCR amplification cycles. Fifteen DAMD primers have been tested for each set. Set (A) produced 89.39% polymorphism level (P%) with polymorphic information content (PIC) average of 0.33 and marker index (MI) average of 3.96. Whereas, in set (B) these values were recorded to be 94.02%, 0.34 and 3.98 for P%, PIC and MI, respectively. Data showed that the two mentioned sets successfully highlighted high polymorphism level between the two studied Salvia sp. This work studies genetic diversity of S. judaica and S. palaestina species using TU-DAMD test as a novel molecular marker.
{"title":"TU-DAMD employment for molecular characterization of Salvia judaica and Salvia palaestina species","authors":"B. Saleh","doi":"10.14232/abs.2021.1.11-16","DOIUrl":"https://doi.org/10.14232/abs.2021.1.11-16","url":null,"abstract":"Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and Salvia palaestina Benth (Palestinian sage) species using touch-up directed amplification of minisatellite region DNA (TU-DAMD) has been performed in two separated sets; in the first set (set A) the initial annealing temperature was increased from 50 °C to 55 °C, whereas, in the second one (set B), it increased from 55 °C to 60 °C by 0.5 °C/cycle during the first 10 PCR amplification cycles. Fifteen DAMD primers have been tested for each set. Set (A) produced 89.39% polymorphism level (P%) with polymorphic information content (PIC) average of 0.33 and marker index (MI) average of 3.96. Whereas, in set (B) these values were recorded to be 94.02%, 0.34 and 3.98 for P%, PIC and MI, respectively. Data showed that the two mentioned sets successfully highlighted high polymorphism level between the two studied Salvia sp. This work studies genetic diversity of S. judaica and S. palaestina species using TU-DAMD test as a novel molecular marker.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.14232/abs.2021.1.17-27
E. E. Shamsabadi, H. Sabouri, H. Soughi, S. J. Sajadi, A. Dadras
The present study was undertaken to analyze diallel data using GGE biplot model to gather information about genetic interrelationships among parents and identification of heterotic combinations for yield and yield components in bread wheat varieties. For this purpose, 8 bread wheat genotypes tested across in half-diallel crosses design, GGE biplot technique was used. Parents included the genotypes of Kouhdasht, Karim, Ehsan, Mehregan, N-92-9, Line 17, N80-19 and Atrak. The hybrids obtained from the one-way cross (28 hybrids) in agricultural years of 2016-17 were evaluated as randomized complete block design in two replications on the research farm of Gonbad Kavous University. The evaluated traits included the grain yield, weight of spike grains, number of grains in spike and number of spikes. Additive main effects and genotype × environment interaction (GGE) were employed in the evaluation of genotypes; analyses showed significant (P< 0.01) G × E, (genotype × environment interaction) with respect to plant seed yield. GGE biplot analysis showed that Karim was as the best general combiners for grain yield, number grain per spike and grain weight per spike, whereas Ehsan had the highest GCA effects for number of spikes. Ehsan and Karim had higher specific combining ability than other genotypes. The studied genotypes for this trait were divided into two heterotic groups where the first group included the genotypes of Kouhdasht, N-92-9, N80-19 and Atrak and the second group contained the genotypes of Line 17, Mehregan and Karim. Mehregan line had a weak combining ability with all testers and N-92-9 had also more power than others. Based on the biplot, the Karim genotype with high general adaptation was introduced as the ideal genotype in terms of grain yield, spike number, grain number per spike and grain weight, so the Karim genotype can be adapted to obtain high yield hybrids.
{"title":"Using of GGE biplot in determination of genetic structure and heterotic groups in wheat (Triticum aestivum L.)","authors":"E. E. Shamsabadi, H. Sabouri, H. Soughi, S. J. Sajadi, A. Dadras","doi":"10.14232/abs.2021.1.17-27","DOIUrl":"https://doi.org/10.14232/abs.2021.1.17-27","url":null,"abstract":"The present study was undertaken to analyze diallel data using GGE biplot model to gather information about genetic interrelationships among parents and identification of heterotic combinations for yield and yield components in bread wheat varieties. For this purpose, 8 bread wheat genotypes tested across in half-diallel crosses design, GGE biplot technique was used. Parents included the genotypes of Kouhdasht, Karim, Ehsan, Mehregan, N-92-9, Line 17, N80-19 and Atrak. The hybrids obtained from the one-way cross (28 hybrids) in agricultural years of 2016-17 were evaluated as randomized complete block design in two replications on the research farm of Gonbad Kavous University. The evaluated traits included the grain yield, weight of spike grains, number of grains in spike and number of spikes. Additive main effects and genotype × environment interaction (GGE) were employed in the evaluation of genotypes; analyses showed significant (P< 0.01) G × E, (genotype × environment interaction) with respect to plant seed yield. GGE biplot analysis showed that Karim was as the best general combiners for grain yield, number grain per spike and grain weight per spike, whereas Ehsan had the highest GCA effects for number of spikes. Ehsan and Karim had higher specific combining ability than other genotypes. The studied genotypes for this trait were divided into two heterotic groups where the first group included the genotypes of Kouhdasht, N-92-9, N80-19 and Atrak and the second group contained the genotypes of Line 17, Mehregan and Karim. Mehregan line had a weak combining ability with all testers and N-92-9 had also more power than others. Based on the biplot, the Karim genotype with high general adaptation was introduced as the ideal genotype in terms of grain yield, spike number, grain number per spike and grain weight, so the Karim genotype can be adapted to obtain high yield hybrids.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-31DOI: 10.14232/abs.2020.1.11-16
S. Ranjini, S. Muniasamy, G. Rameshkumar, T. Rajagopal, T. Sivakumar, P. Ponmanickam
Fishes counteract certain microbial attacks in water by producing antimicrobial proteins/peptides in their skin surface. The present study focused on screening the bactericidal activity of skin and skin mucus extracts of Catla catla and Channa striatus. The bactericidal activity was assessed against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, Aeromonas hydrophila, Staphylococcus aureus and Bacillus coagulans by disc diffusion method. The minimal inhibitory concentration was also determined. Protein profiles in skin and skin mucus extracts were analyzed by SDS-PAGE. Samples from both fishes showed antibacterial activity. Detailed analysis of individual protein and peptide would throw light on their medicinal importance to be used against pathogenic microbes.
{"title":"Bactericidal activity of skin mucus and skin extracts of Catla catla and Channa striatus","authors":"S. Ranjini, S. Muniasamy, G. Rameshkumar, T. Rajagopal, T. Sivakumar, P. Ponmanickam","doi":"10.14232/abs.2020.1.11-16","DOIUrl":"https://doi.org/10.14232/abs.2020.1.11-16","url":null,"abstract":"Fishes counteract certain microbial attacks in water by producing antimicrobial proteins/peptides in their skin surface. The present study focused on screening the bactericidal activity of skin and skin mucus extracts of Catla catla and Channa striatus. The bactericidal activity was assessed against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, Aeromonas hydrophila, Staphylococcus aureus and Bacillus coagulans by disc diffusion method. The minimal inhibitory concentration was also determined. Protein profiles in skin and skin mucus extracts were analyzed by SDS-PAGE. Samples from both fishes showed antibacterial activity. Detailed analysis of individual protein and peptide would throw light on their medicinal importance to be used against pathogenic microbes.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42136107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-27DOI: 10.14232/ABS.2019.2.135-141
B. Saleh
Salvia tomentosa Miller (Lamiaceae) a Mediterranean species has an important role in various pharmacological applications. To reveal genetic relationships among S. tomentosa natural populations, 35 samples were collected from different regions of Syria. Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) markers have been investigated for this goal. Td-DAMD assay produced 158 total bands of which 131 (82.911%) were polymorphic with a mean polymorphic information content (PIC) value of 0.264 and a mean marker index (MI) value of 2.269. Clustering profile based on TdDAMD data showed that samples were grouped into two main clusters; the first cluster included Lattakia samples which split into two subclusters regardless their altitudes over the sea level. Whereas, the second cluster included Tartous and Hama samples. Td-DAMD assay successfully discriminate among the tested 35 samples belonged to the S. tomentosa natural population.
{"title":"Genetic diversity of Salvia tomentosa Miller (Lamiaceae) species using Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) molecular markers","authors":"B. Saleh","doi":"10.14232/ABS.2019.2.135-141","DOIUrl":"https://doi.org/10.14232/ABS.2019.2.135-141","url":null,"abstract":"Salvia tomentosa Miller (Lamiaceae) a Mediterranean species has an important role in various pharmacological applications. To reveal genetic relationships among S. tomentosa natural populations, 35 samples were collected from different regions of Syria. Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) markers have been investigated for this goal. Td-DAMD assay produced 158 total bands of which 131 (82.911%) were polymorphic with a mean polymorphic information content (PIC) value of 0.264 and a mean marker index (MI) value of 2.269. Clustering profile based on TdDAMD data showed that samples were grouped into two main clusters; the first cluster included Lattakia samples which split into two subclusters regardless their altitudes over the sea level. Whereas, the second cluster included Tartous and Hama samples. Td-DAMD assay successfully discriminate among the tested 35 samples belonged to the S. tomentosa natural population.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46137708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-27DOI: 10.14232/ABS.2019.2.125-133
Imen Manaa, R. Djebbar, O. Abrous-Belbachir
Norflurazon 100 µM alone or in combination with α-tocopherol (0.25 mM) was applied in pre-emergence of peanut seedlings (Arachis hypogaea L.). Norflurazon treatment allowed to partially or totally photobleach plants which were noticeably smaller than the control. Norflurazon impaired the photosynthetic activity by decreasing photosynthetic pigments (carotenoids and chlorophylls) and by reducing quantities of soluble sugar. The determination of malondialdehyde (MDA) showed that its content was higher in treated plants in relation with enhancement of reactive oxygen species by the herbicide and decreased the endogenous α-tocopherol. The addition of exogenous α-tocopherol reduced the damage done by the herbicide at the membrane level because of the MDA content was less important than in norflurazon treated seedlings. Furthermore, the norflurazon decreased the glutathione S-transferase (GST) activity in the leaves and the roots of peanut seedlings, while it increased the level of reduced glutathione. This activity decreased even more with the application of exogenous α-tocopherol in combination with the herbicide. The herbicide alone or in association with the antioxidant α-tocopherol increased ascorbic acid content. The supplementation of α-tocopherol did not decrease the phytotoxicity of norflurazon although we observed a decrease in MDA content.
{"title":"Impact of exogenous alpha tocopherol on peanut seedlings (Arachis hypogaea L.) treated by norflurazon","authors":"Imen Manaa, R. Djebbar, O. Abrous-Belbachir","doi":"10.14232/ABS.2019.2.125-133","DOIUrl":"https://doi.org/10.14232/ABS.2019.2.125-133","url":null,"abstract":"Norflurazon 100 µM alone or in combination with α-tocopherol (0.25 mM) was applied in pre-emergence of peanut seedlings (Arachis hypogaea L.). Norflurazon treatment allowed to partially or totally photobleach plants which were noticeably smaller than the control. Norflurazon impaired the photosynthetic activity by decreasing photosynthetic pigments (carotenoids and chlorophylls) and by reducing quantities of soluble sugar. The determination of malondialdehyde (MDA) showed that its content was higher in treated plants in relation with enhancement of reactive oxygen species by the herbicide and decreased the endogenous α-tocopherol. The addition of exogenous α-tocopherol reduced the damage done by the herbicide at the membrane level because of the MDA content was less important than in norflurazon treated seedlings. Furthermore, the norflurazon decreased the glutathione S-transferase (GST) activity in the leaves and the roots of peanut seedlings, while it increased the level of reduced glutathione. This activity decreased even more with the application of exogenous α-tocopherol in combination with the herbicide. The herbicide alone or in association with the antioxidant α-tocopherol increased ascorbic acid content. The supplementation of α-tocopherol did not decrease the phytotoxicity of norflurazon although we observed a decrease in MDA content.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45247021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.14232/ABS.2019.1.25-30
M. Arabi, A. Shoaib, E. Al-shehadah, M. Jawhar
Information on genetic diversity among cultivars is critical in wheat improvement. In this work, heterogeneity within local and introduced cultivars of bread wheat grown in Syria was investigated using amplified fragment length polymorphism (AFLP) markers. The eight primer pairs were used to detect 177 polymorphic bands among the 21 cultivars resulting in an average of 22.13 (57.3%) polymorphic loci per primer pair. Major allelic frequency ranged from 0.50 to 0.75 with a mean 0.64, and estimated gene diversity was 0.45. Values of average polymorphic information content (PIC) for these markers were estimated to be 0.34. This low value might be attributed to the rigorous selection pressure aimed at cultivar purity and associated breeding practices. Dissimilarity values ranged from 0.32 to 0.66 with an average of 0.54, indicating that such techniques sample distinct genome regions. Three major subgroups of wheat cultivars were identified using the unweighted pair-group method with arithmetic means analysis (UPGMA), with all local cultivars falling into one cluster, which was confirmed by a principal component analysis (PCA). The narrow genetic diversity observed among Syrian wheat cultivars suggests the need of broadening the genetic base of wheat breeding materials, including local landraces.
{"title":"Genetic diversity within local and introduced cultivars of wheat (Triticum aestivum L.) grown under Mediterranean environment as revealed by AFLP markers","authors":"M. Arabi, A. Shoaib, E. Al-shehadah, M. Jawhar","doi":"10.14232/ABS.2019.1.25-30","DOIUrl":"https://doi.org/10.14232/ABS.2019.1.25-30","url":null,"abstract":"Information on genetic diversity among cultivars is critical in wheat improvement. In this work, heterogeneity within local and introduced cultivars of bread wheat grown in Syria was investigated using amplified fragment length polymorphism (AFLP) markers. The eight primer pairs were used to detect 177 polymorphic bands among the 21 cultivars resulting in an average of 22.13 (57.3%) polymorphic loci per primer pair. Major allelic frequency ranged from 0.50 to 0.75 with a mean 0.64, and estimated gene diversity was 0.45. Values of average polymorphic information content (PIC) for these markers were estimated to be 0.34. This low value might be attributed to the rigorous selection pressure aimed at cultivar purity and associated breeding practices. Dissimilarity values ranged from 0.32 to 0.66 with an average of 0.54, indicating that such techniques sample distinct genome regions. Three major subgroups of wheat cultivars were identified using the unweighted pair-group method with arithmetic means analysis (UPGMA), with all local cultivars falling into one cluster, which was confirmed by a principal component analysis (PCA). The narrow genetic diversity observed among Syrian wheat cultivars suggests the need of broadening the genetic base of wheat breeding materials, including local landraces.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48302288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.14232/ABS.2019.1.63-68
Mohammad Asadi, H. Rafiee-Dastjerdi, G. Nouri-Ganbalani, B. Naseri, M. Hassanpour
The effects of Allium sativum L. (Alliaceae), Piper nigrum L. (Piperaceae) and Glycyrrhiza glabra L. (Fabaceae) essential oils were investigated on the biological control agent, Habrobracon hebetor Say. The female wasps of H. hebetor were treated by LC30 concentrations of the tested essential oils for 24 h and their demography was investigated. Results indicated that the adult longevity, survival, fecundity, fertility, hatch rate, offspring sex ratio and the other demographic parameters negatively were affected by these essential oils. At the same time, our findings indicated that G. glabra essential oil has the less severe effect on H. hebetor. Accordingly, G. glabra essential oil seems to be a compatible botanical compound with H. hebetor for applying in integrated pest management programs.
{"title":"Insecticidal activity of isolated essential oils from three medicinal plants on the biological control agent, Habrobracon hebetor Say (Hymenoptera: Braconidae)","authors":"Mohammad Asadi, H. Rafiee-Dastjerdi, G. Nouri-Ganbalani, B. Naseri, M. Hassanpour","doi":"10.14232/ABS.2019.1.63-68","DOIUrl":"https://doi.org/10.14232/ABS.2019.1.63-68","url":null,"abstract":"The effects of Allium sativum L. (Alliaceae), Piper nigrum L. (Piperaceae) and Glycyrrhiza glabra L. (Fabaceae) essential oils were investigated on the biological control agent, Habrobracon hebetor Say. The female wasps of H. hebetor were treated by LC30 concentrations of the tested essential oils for 24 h and their demography was investigated. Results indicated that the adult longevity, survival, fecundity, fertility, hatch rate, offspring sex ratio and the other demographic parameters negatively were affected by these essential oils. At the same time, our findings indicated that G. glabra essential oil has the less severe effect on H. hebetor. Accordingly, G. glabra essential oil seems to be a compatible botanical compound with H. hebetor for applying in integrated pest management programs.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48452994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.14232/ABS.2019.1.15-24
S. Saha, T. Dhar, P. Ghosh, T. Dey
The aim of this research was to assess the genetic diversity of sesame (Sesamum indicum L.) and also to reveal the genetic relationships using the Random Amplified Polymorphic DNA (RAPD) markers. Fifteen sesame germplasms were collected from seven districts or four zones of West Bengal, India. A high genetic diversity was revealed by ten RAPD primers within and among the fifteen germplasms. The value of Jaccard’s similarity coefficients among and within the fifteen germplasms ranged from 0.287 to 0.725 which indicated high degree of genetic variability. Cluster analysis using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) grouped all the germplasms into three main clusters. Analysis of various genetic diversity indices strongly indicated high level of genetic diversity among the populations of four different regions. UPGMA analysis of four populations resulted into two groups and the results of Principal Coordinates Analysis (PCoA) depicted a clear distinction among the germplasms.
本研究的目的是利用随机扩增多态性DNA (RAPD)标记分析芝麻(Sesamum indicum L.)的遗传多样性，揭示其遗传关系。从印度西孟加拉邦的7个区或4个区收集了15份芝麻种质。通过10条RAPD引物分析，发现15份种质间具有较高的遗传多样性。15份种质间和种质内的Jaccard相似系数在0.287 ~ 0.725之间，表明遗传变异程度较高。采用UPGMA (Unweighted Pair Group Method with Arithmetic Mean)聚类分析，将所有种质资源划分为3个主要聚类。各遗传多样性指数分析表明，4个不同区域的居群具有较高的遗传多样性。UPGMA分析将4个居群划分为2个类群，主坐标分析(PCoA)结果显示了种质间的明显区别。
{"title":"Molecular characterization of sesame germplasms of West Bengal, India using RAPD markers","authors":"S. Saha, T. Dhar, P. Ghosh, T. Dey","doi":"10.14232/ABS.2019.1.15-24","DOIUrl":"https://doi.org/10.14232/ABS.2019.1.15-24","url":null,"abstract":"The aim of this research was to assess the genetic diversity of sesame (Sesamum indicum L.) and also to reveal the genetic relationships using the Random Amplified Polymorphic DNA (RAPD) markers. Fifteen sesame germplasms were collected from seven districts or four zones of West Bengal, India. A high genetic diversity was revealed by ten RAPD primers within and among the fifteen germplasms. The value of Jaccard’s similarity coefficients among and within the fifteen germplasms ranged from 0.287 to 0.725 which indicated high degree of genetic variability. Cluster analysis using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) grouped all the germplasms into three main clusters. Analysis of various genetic diversity indices strongly indicated high level of genetic diversity among the populations of four different regions. UPGMA analysis of four populations resulted into two groups and the results of Principal Coordinates Analysis (PCoA) depicted a clear distinction among the germplasms.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44084071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.14232/ABS.2019.1.31-36
A. Al-Daoude, E. Al-shehadah, A. Shoiab, M. Jawhar, M. Arabi
Salicylic acid (SA) and phenylalanine ammonia-lyase (PAL) have been suggested as important signals during plant resistance towards several fungal pathogens. In this work, to better understand the defense responses initiated by resistant and susceptible barley genotypes challenged with a necrotrophic (Cochliobolus sativus; Cs) and a biotrophic (Blumeria graminis; Bg) pathogens, the relative contributions of SA and PAL were investigated at early time points of infection. SA signaling was activated in both genotypes 24 hours post infection (hpi) as compared with the non-inoculated plants. However, with or without pathogen pretreatment, SA significantly increased (P = 0.001) in the resistant genotype that contained three-folds of total SA in comparison with the susceptible one for Bg. Reverse transcription-polymerase chain reaction (RTPCR) analysis revealed that PAL expression increases in the resistant and susceptible genotypes over the inoculation time points, with the maximum expression observed 48 hpi. PAL expression was paralleled by an increase in SA content in leaves as shown by the test coincidence (F3, 32 = 1.09, P = 0.49 for Cs and F3, 32 = 1.03, P = 0.48 for Bg). Results showed that the cooperatively function of SA and PAL in barley responses to both Cs and Bg appeared to be dependent on the plant genotype, and that SA signaling and PAL play a role in barley interactions with these both pathogens. This study might increase our understanding for a deeper molecular research on barley defense responses against pathogens with different lifestyles.
水杨酸(SA)和苯丙氨酸解氨酶(PAL)被认为是植物抵抗几种真菌病原体的重要信号。在这项工作中，为了更好地了解抗性和易感大麦基因型受到坏死性玉米萎蔫病(Cochliobolus sativus;c)和一种生物营养(Blumeria graminis;Bg)病原菌，在感染早期观察SA和PAL的相对贡献。与未接种的植株相比，两种基因型的SA信号在感染后24小时被激活。然而，无论是否对病原菌进行预处理，含有3倍总SA的抗性基因型与Bg敏感基因型相比，SA显著增加(P = 0.001)。逆转录聚合酶链反应(RTPCR)分析显示，在接种时间点上，抗性和敏感基因型的PAL表达量均有所增加，最高表达量为48 hpi。PAL的表达与叶片中SA含量的增加呈平行关系(F3, 32 = 1.09, P = 0.49, F3, 32 = 1.03, P = 0.48)。结果表明，SA和PAL在大麦对Cs和Bg的反应中的协同作用似乎依赖于植物基因型，SA信号和PAL在大麦与这两种病原体的相互作用中起作用。本研究为深入研究大麦对不同生活方式病原菌的防御反应提供了新的思路。
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