Pub Date : 2019-01-30DOI: 10.14232/abs.2018.2.115-122
G. Feigl, Á. Bordé, Á. Molnár, Z. Kolbert
Due to the intensified production of reactive nitrogen species (RNS) proteins can be modified by tyrosine nitration (PTN). Examination of PTN is a hot topic of plant biology, especially because the exact outcome of this modification is still pending. Both RNS and ascorbic acid (AsA) are redox-active molecules, which directly affect the redox state of cells. The possible link between RNS-dependent PTN and AsA metabolism was studied in RNS (gsnor1-3, nia1nia2) and AsA (vtc2-3) homeostasis Arabidopsis mutants. During physiological conditions, intensified PTN was detected in all mutant lines compared to the wild-type (WT); without altering nitration pattern. Moreover, the increased PTN seemed to be associated with endogenous peroxynitrite (ONOO-) levels, but it showed no tight correlation with endogenous levels of nitric-oxide (NO) or AsA. Exogenous AsA caused intensified PTN in WT, vtc2-3 and nia1nia2. In the background of increased PTN, significant NO and ONOO- accumulation was detected, indicating exogenous AsA-induced RNS burst. Interestingly, in AsA-triggered stress-situation, changes of NO levels seem to be primarily connected to the development of PTN. Our results point out for the first time that similarly to human and animal systems exogenous AsA exerts pro-nitrant effect on plant proteome.
{"title":"Exogenous ascorbic acid is a pro-nitrant in Arabidopsis thaliana","authors":"G. Feigl, Á. Bordé, Á. Molnár, Z. Kolbert","doi":"10.14232/abs.2018.2.115-122","DOIUrl":"https://doi.org/10.14232/abs.2018.2.115-122","url":null,"abstract":"Due to the intensified production of reactive nitrogen species (RNS) proteins can be modified by tyrosine nitration (PTN). Examination of PTN is a hot topic of plant biology, especially because the exact outcome of this modification is still pending. Both RNS and ascorbic acid (AsA) are redox-active molecules, which directly affect the redox state of cells. The possible link between RNS-dependent PTN and AsA metabolism was studied in RNS (gsnor1-3, nia1nia2) and AsA (vtc2-3) homeostasis Arabidopsis mutants. During physiological conditions, intensified PTN was detected in all mutant lines compared to the wild-type (WT); without altering nitration pattern. Moreover, the increased PTN seemed to be associated with endogenous peroxynitrite (ONOO-) levels, but it showed no tight correlation with endogenous levels of nitric-oxide (NO) or AsA. Exogenous AsA caused intensified PTN in WT, vtc2-3 and nia1nia2. In the background of increased PTN, significant NO and ONOO- accumulation was detected, indicating exogenous AsA-induced RNS burst. Interestingly, in AsA-triggered stress-situation, changes of NO levels seem to be primarily connected to the development of PTN. Our results point out for the first time that similarly to human and animal systems exogenous AsA exerts pro-nitrant effect on plant proteome.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48609722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-30DOI: 10.14232/ABS.2018.2.123-129
F. Koohdar, F. Attar, S. Talebi, M. Sheidai
Dracocephalum is the second largest genus in the family Lamiaceae with about 186 species. These species are native in temperate regions of the Northern Hemisphere and occur in the territory of the extra-tropical Asia and Europe. Eight Dracocephalum species reported in Iran; these are mainly growing in the northern and central parts of the country belonging to the Irano-Turanian phytogeographical region. Dracocephalum kotschyi is an important medicinal plant .in the country. At the same time, taxonomic position of Dracocephalum oligadenium is a challenging issue. In this work morphological, anatomical and Inter Simple Sequence Repeat (ISSR) markers were used to identify these species in Iran. MDS plot based on morphological and anatomical characters, furthermore, PCoA and MST plot based on ISSR data of species revealed hybridization between D. oligadenium and D. kotschyi.
{"title":"Contemporary interspecific hybridization between Dracocephalum kotschyi and Dracocephalum oligadenium (Lamiaceae): Evidence from morphological, anatomical and molecular data","authors":"F. Koohdar, F. Attar, S. Talebi, M. Sheidai","doi":"10.14232/ABS.2018.2.123-129","DOIUrl":"https://doi.org/10.14232/ABS.2018.2.123-129","url":null,"abstract":"Dracocephalum is the second largest genus in the family Lamiaceae with about 186 species. These species are native in temperate regions of the Northern Hemisphere and occur in the territory of the extra-tropical Asia and Europe. Eight Dracocephalum species reported in Iran; these are mainly growing in the northern and central parts of the country belonging to the Irano-Turanian phytogeographical region. Dracocephalum kotschyi is an important medicinal plant .in the country. At the same time, taxonomic position of Dracocephalum oligadenium is a challenging issue. In this work morphological, anatomical and Inter Simple Sequence Repeat (ISSR) markers were used to identify these species in Iran. MDS plot based on morphological and anatomical characters, furthermore, PCoA and MST plot based on ISSR data of species revealed hybridization between D. oligadenium and D. kotschyi.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42094901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-30DOI: 10.14232/ABS.2018.2.170-179
I. Rusyn, Кhrystyna R. Hamkalo
The paper descibes the development of a biotechnological system for generating bioelectricity on closed balconies of buildings from living plants Alisma plantago-aquatica and soil microorganisms grown in containers with natural wetland substrate, provided with a graphite and Zn-galvanized steel electrode system. This biotechnology worked efficiently from the first days after installation and was practically at full capacity 2 weeks later. Electric power output was highest in the spring-summer and the early autumn period (at the time of the highest photosynthetic activity of plants). The highest current output was 58.6 mA at 10 Ω load. Bioelectricity generation by the biosystem was stable with slight fluctuations throughout the year in well-lighted and heated premises at a temperature of 21-26 °C, and the seasonal reduction of the bioelectricity level was 8.71%. On not-heated closed terraces and glazed balconies, with temperature fluctuations from 5 to 26 °C, the electricity production decreased in the winter period by 19.98% and 39.91% with and without adding of sulfate-reducing bacteria, respectively. The proposed system of electrodes for collection of bioelectric power is new, easy to manufacture and economical. It is resistant to waterlogged environment, and has good prospects for further improvements for more effective collection of plant-microbial bioelectricity. Maintainance of the biosystem is simple and accessible to everyone without special skills.
{"title":"Bioelectricity production in an indoor plant-microbial biotechnological system with Alisma plantago-aquatica","authors":"I. Rusyn, Кhrystyna R. Hamkalo","doi":"10.14232/ABS.2018.2.170-179","DOIUrl":"https://doi.org/10.14232/ABS.2018.2.170-179","url":null,"abstract":"The paper descibes the development of a biotechnological system for generating bioelectricity on closed balconies of buildings from living plants Alisma plantago-aquatica and soil microorganisms grown in containers with natural wetland substrate, provided with a graphite and Zn-galvanized steel electrode system. This biotechnology worked efficiently from the first days after installation and was practically at full capacity 2 weeks later. Electric power output was highest in the spring-summer and the early autumn period (at the time of the highest photosynthetic activity of plants). The highest current output was 58.6 mA at 10 Ω load. Bioelectricity generation by the biosystem was stable with slight fluctuations throughout the year in well-lighted and heated premises at a temperature of 21-26 °C, and the seasonal reduction of the bioelectricity level was 8.71%. On not-heated closed terraces and glazed balconies, with temperature fluctuations from 5 to 26 °C, the electricity production decreased in the winter period by 19.98% and 39.91% with and without adding of sulfate-reducing bacteria, respectively. The proposed system of electrodes for collection of bioelectric power is new, easy to manufacture and economical. It is resistant to waterlogged environment, and has good prospects for further improvements for more effective collection of plant-microbial bioelectricity. Maintainance of the biosystem is simple and accessible to everyone without special skills.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46379260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-30DOI: 10.14232/abs.2018.2.158-168
A. Ganguly, A. Banerjee, A. Mandal, P. Mohapatra
Clarias batrachus (Linn.) is widely recognized in Indian sub-continent for its nutritional and economic significance. At present, it remains at a merely vulnerable state. Pathogenic infections, diminution of natural habitats and introduction of allied exotic fishes are the causes of productivity constraint, particularly in Southern Asia. Conversely, African cat fish Clarias gariepinus has been significantly identified as a potential threat to biodiversity, despite being its large scale cultivation across the world. Thus emphasis on indigenous C. batrachus farming is becoming inevitable. Currently, screening of autochthonous probiotic organisms for the cultivation of C. batrachus in semi-intensive manner is getting importance. At the same time, molecular omics-based technologies are also gaining considerable attention to identify potential probiotic markers. This review provides an overall concept of probiotics, its application and future perspectives in relation to the cultivation of C. batrachus.
{"title":"Probiotic-based cultivation of Clarias batrachus: importance and future perspective","authors":"A. Ganguly, A. Banerjee, A. Mandal, P. Mohapatra","doi":"10.14232/abs.2018.2.158-168","DOIUrl":"https://doi.org/10.14232/abs.2018.2.158-168","url":null,"abstract":"Clarias batrachus (Linn.) is widely recognized in Indian sub-continent for its nutritional and economic significance. At present, it remains at a merely vulnerable state. Pathogenic infections, diminution of natural habitats and introduction of allied exotic fishes are the causes of productivity constraint, particularly in Southern Asia. Conversely, African cat fish Clarias gariepinus has been significantly identified as a potential threat to biodiversity, despite being its large scale cultivation across the world. Thus emphasis on indigenous C. batrachus farming is becoming inevitable. Currently, screening of autochthonous probiotic organisms for the cultivation of C. batrachus in semi-intensive manner is getting importance. At the same time, molecular omics-based technologies are also gaining considerable attention to identify potential probiotic markers. This review provides an overall concept of probiotics, its application and future perspectives in relation to the cultivation of C. batrachus.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45565188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Chatterjee, C. Malick, S. Bhattacharya, Rakesh Kundu, V. R. Suresh, S. K. Saikia
Hilsa, Tenualosa ilisha is an amphihaline migratory fish that performs spawning migration to selected freshwater rivers in Indo-Pacific region. It is not clear what force triggers its migration. In this paper, we attempted to describe the features of outer integument from its head region as chemosensory site which appears to play significant role in its upstream migration. We found that this area (termed as snout) has very soft and scale less tissue oriented with pit like grooves named as ‘epidermal pit’. Around these pits, odorant receptor G-protein subunits (Gαq, Gαs/olf and Gαo) have been substantially localized. Use of DASPEI also traced this area with neuronal existence. These features in the snout likely to contribute for chemosensory requirements of the fish during upstream migration. Considering such findings, we named this area of snout as ‘sensory pad’. Its position at the forefront of olfactory organ and brain may have important role in facilitating sensory reception by the fish swimming upstream to the river.
{"title":"‘Sensory pad’- A novel chemoreceptive device in Hilsa (Tenualosa ilisha) to support its amphihaline attribute","authors":"S. Chatterjee, C. Malick, S. Bhattacharya, Rakesh Kundu, V. R. Suresh, S. K. Saikia","doi":"10.14232/ABS.2018.1.1-6","DOIUrl":"https://doi.org/10.14232/ABS.2018.1.1-6","url":null,"abstract":"Hilsa, Tenualosa ilisha is an amphihaline migratory fish that performs spawning migration to selected freshwater rivers in Indo-Pacific region. It is not clear what force triggers its migration. In this paper, we attempted to describe the features of outer integument from its head region as chemosensory site which appears to play significant role in its upstream migration. We found that this area (termed as snout) has very soft and scale less tissue oriented with pit like grooves named as ‘epidermal pit’. Around these pits, odorant receptor G-protein subunits (Gαq, Gαs/olf and Gαo) have been substantially localized. Use of DASPEI also traced this area with neuronal existence. These features in the snout likely to contribute for chemosensory requirements of the fish during upstream migration. Considering such findings, we named this area of snout as ‘sensory pad’. Its position at the forefront of olfactory organ and brain may have important role in facilitating sensory reception by the fish swimming upstream to the river.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45562187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.14232/ABS.2018.1.75-82
Riddha Mukherjee, Tanmay Paul, S. Halder, J. P. Soren, A. Banerjee, K. C. Mondal, B. Pati, P. Mohapatra
An acidophilic α-amylase from Aspergillus niger RBP7 was purified after solid state fermentation on potato peel substrate. Molecular mass of the purified α-amylase was 37.5 kDa and it exhibited 1.4 mg/ml and 0.992 μ/mol/min Km and Vmax values, respectively. The enzyme was stable in the pH range from 2.0 to 6.0, at high NaCl concentration (3 M) and at temperatures between 40 °C and 70 °C. The enzyme showed an optimal activity at pH 3.0 and at 45 °C. The enzyme was inhibited by Hg2+ and was stable in the presence of different surfactants (Tween 60, Tween 80, and SDS at 1% level) and different inhibitory reagents (β-mercaptoethanol, phenylmethylsulfonyl fluoride, and sodium azide). This acidophilic amylase enzyme can digest heterogeneous food materials, i.e. the mixture of rice, fish, bread and curry with comparable activity to the commercial diastase enzymes available.
{"title":"Characterization of an acidophilic α-amylase from Aspergillus niger RBP7 and study of catalytic potential in response to nutritionally important heterogeneous compound","authors":"Riddha Mukherjee, Tanmay Paul, S. Halder, J. P. Soren, A. Banerjee, K. C. Mondal, B. Pati, P. Mohapatra","doi":"10.14232/ABS.2018.1.75-82","DOIUrl":"https://doi.org/10.14232/ABS.2018.1.75-82","url":null,"abstract":"An acidophilic α-amylase from Aspergillus niger RBP7 was purified after solid state fermentation on potato peel substrate. Molecular mass of the purified α-amylase was 37.5 kDa and it exhibited 1.4 mg/ml and 0.992 μ/mol/min Km and Vmax values, respectively. The enzyme was stable in the pH range from 2.0 to 6.0, at high NaCl concentration (3 M) and at temperatures between 40 °C and 70 °C. The enzyme showed an optimal activity at pH 3.0 and at 45 °C. The enzyme was inhibited by Hg2+ and was stable in the presence of different surfactants (Tween 60, Tween 80, and SDS at 1% level) and different inhibitory reagents (β-mercaptoethanol, phenylmethylsulfonyl fluoride, and sodium azide). This acidophilic amylase enzyme can digest heterogeneous food materials, i.e. the mixture of rice, fish, bread and curry with comparable activity to the commercial diastase enzymes available.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45909664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.14232/abs.2018.1.25-36
M. E. Kherraze, M. Belhamra, M. Grigore
This study focuses on the anatomical strategies developed by the Traganum nudatum Del., prevalent in the Algerian Sahara, particularly in the region of Oued Righ, which allows to this species to survive in a harsh environment (aridity and salinity). The anatomical structure of this species was studied using fresh materials (roots, stems and leaves). These materials have been collected from several individuals in different saline habitats. Some interesting features such as successive cambia phenomenon, calcium oxalate crystals, Kranz anatomy (salsoloid subtype), succulence, low stomata density, low stomata index, the presence of the papillae, paracytic stomata and other structures have been noticed. We can conclude that the ecological significance of evidenced adaptations by T. nudatum is supported in this article by the analysis of adaptations of other species belonging either to the Amaranthaceae or to other botanical families; and that this adaptation has no link with botanical families. In these species, the key adaptation is the ability to maintain growth processes and water saving under difficult living conditions (high summer temperatures or salty soils), regardless of the evolutionary level of the taxon.
{"title":"Aspects of ecological anatomy of Traganum nudatum Del. (Amaranthaceae) from the Northeast of the Algerian Sahara","authors":"M. E. Kherraze, M. Belhamra, M. Grigore","doi":"10.14232/abs.2018.1.25-36","DOIUrl":"https://doi.org/10.14232/abs.2018.1.25-36","url":null,"abstract":"This study focuses on the anatomical strategies developed by the Traganum nudatum Del., prevalent in the Algerian Sahara, particularly in the region of Oued Righ, which allows to this species to survive in a harsh environment (aridity and salinity). The anatomical structure of this species was studied using fresh materials (roots, stems and leaves). These materials have been collected from several individuals in different saline habitats. Some interesting features such as successive cambia phenomenon, calcium oxalate crystals, Kranz anatomy (salsoloid subtype), succulence, low stomata density, low stomata index, the presence of the papillae, paracytic stomata and other structures have been noticed. We can conclude that the ecological significance of evidenced adaptations by T. nudatum is supported in this article by the analysis of adaptations of other species belonging either to the Amaranthaceae or to other botanical families; and that this adaptation has no link with botanical families. In these species, the key adaptation is the ability to maintain growth processes and water saving under difficult living conditions (high summer temperatures or salty soils), regardless of the evolutionary level of the taxon.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41847581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.14232/ABS.2018.1.37-43
M. Sheidai, S. Shojaei, F. Koohdar
The genus Cirsium Mill. (Asteraceae) also known as plume thistles with about 250 perennial, biennial or rarely annual spiny species is a phylogenetically unresolved and paraphyletic genus. Cirsium species grow in different ecological conditions and tend to form interspecific hybrids. Some species are morphologically very similar and need to be delineated by additional anatomical and molecular characters. About 28 Cirsium species have been reported in Flora Iranica; they were classified in five sections. Taxonomic investigation of these species was confined to morphology and molecular study of RAPD and ISSR markers. The present study carried out anatomical investigation (leaf and stem characters) of Cirsium species in the country. PCoA analysis of anatomical characters could delimit the studied species and the grouping obtained was almost in agreement with morphological and sectional delineation of the genus. The results obtained are in agreement with several other investigations and all together suggestive of the continued gene flow and introgression between Cirsium species that make taxonomy and phylogenetic relationship of the species difficult.
{"title":"Anatomy study of the genus Cirsium Mill. in Iran","authors":"M. Sheidai, S. Shojaei, F. Koohdar","doi":"10.14232/ABS.2018.1.37-43","DOIUrl":"https://doi.org/10.14232/ABS.2018.1.37-43","url":null,"abstract":"The genus Cirsium Mill. (Asteraceae) also known as plume thistles with about 250 perennial, biennial or rarely annual spiny species is a phylogenetically unresolved and paraphyletic genus. Cirsium species grow in different ecological conditions and tend to form interspecific hybrids. Some species are morphologically very similar and need to be delineated by additional anatomical and molecular characters. About 28 Cirsium species have been reported in Flora Iranica; they were classified in five sections. Taxonomic investigation of these species was confined to morphology and molecular study of RAPD and ISSR markers. The present study carried out anatomical investigation (leaf and stem characters) of Cirsium species in the country. PCoA analysis of anatomical characters could delimit the studied species and the grouping obtained was almost in agreement with morphological and sectional delineation of the genus. The results obtained are in agreement with several other investigations and all together suggestive of the continued gene flow and introgression between Cirsium species that make taxonomy and phylogenetic relationship of the species difficult.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41581909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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