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A computational enzymatic optimization for fixing carbon dioxide to starch 将二氧化碳固定到淀粉中的计算酶优化方法
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241000
Rong Gao
Carbon dioxide is fixed and processed into starch by the plants photosynthesis through complicated molecular pathways. While planting and cultivating crops are the major ways to harvest starch, an artificial anabolic pathway has recently been realized in China. Traditional crop production demands extended harvest periods, extensive land, and substantial water use. In contrast, the artificial pathway enhances starch synthesis efficiently, using fewer resources for a more sustainable approach. In 2021, Chinese researchers reported the anabolic starch artificial pathway (ASAP) to synthesize starch in vitro. Although the previous research established a milestone, steps need to be optimized. In this work, enzymatic starch synthesis is chosen to be further engineered, building mutants with similar catalytic functions. Computational tools are used to build an iterative docking-mutating simulation (IDMS). It can automatically finish the cycle of protein mutations and docking. Autodock and Rosetta are used in the coding. 445 different protein mutants are generated and analyzed in silico, among which the best five were chosen for experimental investigation. In the experimental analysis, mutant E shows nearly the same catalytic efficiency as the wild-type in the first hour, with a 2.5-fold expression rate.
植物的光合作用通过复杂的分子途径将二氧化碳固定并加工成淀粉。种植和栽培农作物是收获淀粉的主要途径,而中国最近实现了人工合成代谢途径。传统的农作物生产需要延长收获期,占用大量土地和水资源。相比之下,人工合成途径能有效提高淀粉合成效率,使用更少的资源,是一种更可持续的方法。2021 年,中国研究人员报道了体外合成淀粉的合成代谢淀粉人工途径(ASAP)。虽然前人的研究建立了一个里程碑,但还需要优化步骤。本研究选择酶法淀粉合成进行进一步工程化,构建具有相似催化功能的突变体。利用计算工具建立了迭代对接-突变模拟(IDMS)。它可以自动完成蛋白质突变和对接的循环。编码中使用了 Autodock 和 Rosetta。生成并分析了 445 种不同的蛋白质突变体,并选择其中最好的 5 种进行实验研究。在实验分析中,突变体 E 在第一个小时内显示出与野生型几乎相同的催化效率,表达率为野生型的 2.5 倍。
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引用次数: 0
Intelligent monitoring of surface evapotranspiration in the Heihe River Basin based on SEBS modelling 基于 SEBS 模型的黑河流域地表蒸散量智能监测
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20240996
Zijie Pang, Kehao Su
[Objective] The Heihe River Basin, located in northwestern China, is the second largest inland basin in China, and its water resources play a crucial role in the ecology, agriculture and human life of the region. The aim of this study is to investigate the spatial and temporal variations of surface evapotranspiration in the Heihe River Basin and the potential impacts of these variations on water resource management. [Methods] To achieve this goal, we applied the PM-based dual-source model, a meteorological model for estimating global surface ET, which takes into account a variety of factors such as temperature, humidity, wind speed and downward solar shortwave radiation. By analysing the meteorological data and remote sensing data of the Black River Basin, we first investigated the spatial and temporal distribution of surface evapotranspiration. [Conclusion] The results show that surface evapotranspiration shows obvious seasonal and regional variations and is significantly affected by meteorological conditions. The inversion of surface ET in the Heihe River Basin by this dual-source model needs to be improved, and the trend of ET values calculated by the model is relatively small compared with the actual values.
[目的] 位于中国西北部的黑河流域是中国第二大内陆流域,其水资源对该地区的生态、农业和人类生活起着至关重要的作用。本研究旨在探讨黑河流域地表蒸散量的时空变化及其对水资源管理的潜在影响。[方法] 为了实现这一目标,我们应用了基于 PM 的双源模型,这是一种用于估算全球地表蒸散发的气象模型,考虑了温度、湿度、风速和向下的太阳短波辐射等多种因素。通过分析黑河流域的气象数据和遥感数据,我们首先研究了地表蒸散发的时空分布。[结论]研究结果表明,地表蒸散发具有明显的季节性和区域性变化,受气象条件影响较大。该双源模型对黑河流域地表蒸散发的反演能力有待提高,模型计算的蒸散发值与实际值相比变化趋势较小。
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引用次数: 0
Analysis of large-scale high-quality graphene production and applications 大规模高质量石墨烯生产和应用分析
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20240999
Sijia Li
Graphene, a single-atom-thick layer of carbon atoms arranged in a hexagonal lattice, is the thinnest and strongest material known to mankind. It has excellent electrical, thermal, and mechanical properties, making it a promising material for a wide range of applications in electronics, optoelectronics, energy storage, and more. With the increasing demand for graphene in various applications, large-scale and high-quality graphene production has become a significant challenge. While early methods of graphene production involved mechanical exfoliation, this method is limited in terms of scalability and yield. To meet the increasing demand for large-scale production of graphene, various methods have been developed in recent years, including chemical vapor precipitation, epitaxial crystal growth, graphene oxide reduction and solvent exfoliation and so on. This study aims to introduce several existing methods for the mass production of graphene with high quality and analyzes the advantages and disadvantage involve thereof. The findings in this paper may provide a valuable reference for the industrial-scale production of graphene.
石墨烯是一层单原子厚的碳原子,呈六角形晶格排列,是人类已知最薄、强度最高的材料。石墨烯具有优异的电学、热学和机械特性,是一种在电子、光电、储能等领域具有广泛应用前景的材料。随着各种应用领域对石墨烯需求的不断增加,大规模、高质量的石墨烯生产已成为一项重大挑战。虽然早期的石墨烯生产方法涉及机械剥离,但这种方法在可扩展性和产量方面受到限制。为了满足石墨烯大规模生产日益增长的需求,近年来人们开发了多种方法,包括化学气相沉淀法、外延晶体生长法、氧化石墨烯还原法和溶剂剥离法等。本研究旨在介绍现有的几种大规模生产高质量石墨烯的方法,并分析其优缺点。本文的研究结果可为石墨烯的工业化生产提供有价值的参考。
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引用次数: 0
Hierarchical porous carbon with abundant N/O doping as an anode for zinc ion hybrid supercapacitors 掺杂大量 N/O 的分层多孔碳作为锌离子混合超级电容器的阳极
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241005
Yuchen Li, Shun Yang, Penghao Chai, Jianghuan Li, Qiulong Guan, Lixia Bao, Jiong Pen, Wensheng Deng, Xin Li
Aqueous zinc-ion hybrid supercapacitors (ZISCs) known for their affordability, stability, and high energy density represent innovative energy storage devices. Porous carbon is usually used as the cathode material of ZISCs, and its structure significantly affects the dual performance of high power density and energy density of ZISCs. Herein, a one-pot carbonization strategy is proposed, eliminating the need for templates, additional heteroatom compounds, and activation processes. By precisely controlling the temperature to optimize the structure and electrochemical performance of carbon materials, we successfully synthesized hierarchical porous carbon materials (NOPC-800) with a high specific surface area of 1545.7 mg, featuring dual doping of 12.3 at% nitrogen and 13.35 at% oxygen. The self-doping of abundant nitrogen and oxygen atoms facilitates the chemical adsorption of ions and accelerates pseudocapacitive reaction kinetics. Leveraging these advantages, ZISCs were assembled using NOPC-800 as the positive electrode and zinc as the negative electrode, showcasing remarkable performance: a specific capacity of up to 121.9 mAh g, an energy density of 97.5 Wh kg, and a power density of up to 16000 W kg. Remarkably, NOPC-800 maintained an excellent capacity retention of 94.9% after 10,000 cycles at a current density of 10 A g. This research paves an innovative and feasible path for the design and advancement of novel heteroatom-rich carbon cathodes.
水性锌离子混合超级电容器(ZISC)以其经济性、稳定性和高能量密度而著称,是一种创新的能量存储设备。多孔碳通常用作 ZISC 的阴极材料,其结构对 ZISC 的高功率密度和高能量密度的双重性能有很大影响。本文提出了一种一锅碳化策略,无需模板、额外的杂原子化合物和活化过程。通过精确控制温度来优化碳材料的结构和电化学性能,我们成功合成了比表面积高达 1545.7 毫克的分层多孔碳材料(NOPC-800),其中氮和氧的掺杂比例分别为 12.3% 和 13.35%。大量氮原子和氧原子的自掺杂促进了离子的化学吸附,并加速了伪电容反应动力学。利用这些优势,以 NOPC-800 为正极、锌为负极组装而成的 ZISC 表现出卓越的性能:比容量高达 121.9 mAh g,能量密度为 97.5 Wh kg,功率密度高达 16000 W kg。值得注意的是,在 10 A g 的电流密度下,NOPC-800 经过 10,000 次循环后仍能保持 94.9% 的出色容量保持率。这项研究为设计和开发新型富含杂原子的碳阴极铺平了一条创新而可行的道路。
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引用次数: 0
A review of catalytic reduction of carbon dioxide 二氧化碳催化还原综述
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241001
Jiajie Shen
Carbon dioxide (CO2) generated from the consumption of fossil fuels is one of the main factors contributing to the greenhouse effect. How to mitigate the greenhouse effect and reduce the extraction and consumption of fossil fuels has become an issue worthy of study. In recent years, a technology for the catalytic reduction of CO2 to fossil fuels and chemicals has attracted a great deal of attention by providing a new way to solve this problem. This paper reviews the basic principles of three fundamental catalytic CO2 reduction, photocatalysis, electrocatalysis and thermocatalysis, and two advanced catalytic modes, thermocatalysis and photoelectrocatalysis, derived from these three modes, as well as their advantages and disadvantages. In addition, the challenges that the catalytic reduction of CO2 is currently facing are also discussed. Although this technology has made significant progress over the decades, it is still in its preliminary stage with some shortcomings and there is a long way to go before it can be applied in reality.
消耗化石燃料产生的二氧化碳(CO2)是造成温室效应的主要因素之一。如何缓解温室效应,减少化石燃料的开采和消耗,已成为一个值得研究的问题。近年来,一种将二氧化碳催化还原为化石燃料和化学品的技术为解决这一问题提供了新途径,引起了广泛关注。本文综述了光催化、电催化和热催化三种基本催化还原二氧化碳的基本原理,以及由这三种模式衍生出的热催化和光电催化两种先进催化模式及其优缺点。此外,还讨论了催化还原二氧化碳目前面临的挑战。虽然这项技术在过去几十年中取得了重大进展,但仍处于初级阶段,存在一些不足之处,距离实际应用还有很长的路要走。
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引用次数: 0
Sustainable management of water resources in arid areas 干旱地区水资源的可持续管理
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241016
Kaiyue Jiang
Water is an essential resource for human survival, production and socio-economic development, essential for all forms of life, and essential for the maintenance of healthy ecosystems. Due to the continuous change of climate and the continuous growth of population, economy, region and other factors, water resources are becoming more and more scarce. In arid and semi-arid regions, the coupling and interaction between water resources security, energy security, food security and ecological security are becoming more complex, conflicts and contradictions are gradually emerging or intensifying, and uncertainty and risk levels are increasing. Water utilization and management methods are also becoming increasingly serious and complex, which requires new and innovative approaches to conserve and use water wisely. The sustainable management of water resources has always been the focus of research on water resources in the world, and there is much research on water resources management in major forums. This paper uses the method of review to analyze the relevant articles on water resources management, and puts forward more effective methods for sustainable water resources management, and provides suggestions for follow-up research on sustainable water resources management.
水是人类生存、生产和社会经济发展的重要资源,是各种形式的生命所必需的,也是维持健康的生态系统所必需的。由于气候的不断变化以及人口、经济、地区等因素的持续增长,水资源日益匮乏。在干旱半干旱地区,水资源安全、能源安全、粮食安全、生态安全之间的耦合与互动关系日趋复杂,冲突和矛盾逐渐显现或加剧,不确定性和风险水平不断提高。水资源的利用和管理方式也日趋严峻和复杂,这就需要有新的创新方法来节约用水、合理用水。水资源的可持续管理一直是世界水资源研究的重点,各大论坛对水资源管理的研究也很多。本文采用综述的方法,对水资源管理的相关文章进行了分析,提出了更多水资源可持续管理的有效方法,并对水资源可持续管理的后续研究提出了建议。
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引用次数: 0
Historical and Future global warming under different RCP emission scenarios 不同 RCP 排放情景下的历史和未来全球变暖情况
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20240991
Liminhao Xue
Understanding the relationship between carbon emissions and climate change has become increasingly urgent amidst the universal endorsement of sustainable development and the establishment of net-zero carbon emission target (The Paris Agreement). In this study, model projections based on Coupled Model Intercomparison Project Phase 5 (CMIP5) are used to examine future climate change under different representative concentration pathway (RCP) emission scenarios till 2300. Besides analysis of future global average temperature change under the emission scenarios of RCP2.6, RCP4.5, RCP6.0, and RCP 8.5, historical data is first collected during the past three decades (1990-2021). The results show that the simulated global warming ranges from 0.9 to 1.3 between 1990 and 2021, consistent with the observations (0.93). Carbon emissions in China arose mostly from manufacturing and construction, electricity and heat and transport. Emissions from electricity, heat and transport increase by a high ratio of 7.7 and 3.5 times due to industry development. Under different RCP scenarios, the global surface temperature anomaly will increase by 0.2-1.0, 1.2-2.0, 3.5-4.3 and 3.0-8.5 , respectively. The surface temperature anomaly values under RCP8.5 are much higher than those under other emission scenarios. Only in the RCP2.6 scenario (sustainability), the surface warming in all simulations stays below a 2 threshold at the end of the 21st century. To attain net zero emissions, it is highly demanded to develop strict emission policies, chart a course toward decarbonization, and institute effective measures aimed at realizing net zero emissions within the short to medium term. Concurrently striving for economic objectives, managing the equilibrium between economic growth and environmental sustainability emerges as a critical imperative.
随着可持续发展和净碳零排放目标(《巴黎协定》)的确立得到普遍认可,了解碳排放与气候变化之间的关系变得日益迫切。本研究采用基于耦合模式相互比较项目第 5 阶段(CMIP5)的模式预测,研究 2300 年前不同代表性浓度路径(RCP)排放情景下的未来气候变化。除了分析 RCP2.6、RCP4.5、RCP6.0 和 RCP8.5 排放情景下的未来全球平均气温变化外,还首先收集了过去三十年(1990-2021 年)的历史数据。结果表明,1990 年至 2021 年期间,模拟的全球变暖范围在 0.9 至 1.3 之间,与观测值(0.93)一致。中国的碳排放主要来自制造业和建筑业、电力和热力以及交通运输业。由于工业的发展,电力、热力和交通的排放量分别增加了 7.7 倍和 3.5 倍。在不同的 RCP 情景下,全球地表温度异常值将分别增加 0.2-1.0、1.2-2.0、3.5-4.3 和 3.0-8.5。RCP8.5 下的地表温度畸变值远远高于其他排放情景下的地表温度畸变值。只有在 RCP2.6 情景下(可持续性),所有模拟的地表升温在 21 世纪末都保持在 2 临界值以下。要实现净零排放,就必须制定严格的排放政策,制定去碳化路线,并采取有效措施,在中短期内实现净零排放。在努力实现经济目标的同时,管理好经济增长与环境可持续性之间的平衡也是当务之急。
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引用次数: 0
Review on preheating systems for Lithium-ion batteries of electric vehicles under low temperature circumstance 低温环境下电动汽车锂离子电池预热系统综述
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241006
Yansen Li
Nowadays, energy and environmental protection issues have become the focus of most attention. The development of electric vehicles which depends on lithium ion battery as power source is one of the most applicable way in dealing with these issues. Lithium-ion battery (LIB) is highly favoured for its outstanding features in energy density, cycle life and energy retention. However, its severe sensitivity to working temperatures leads to problems when driving electric vehicles. Therefore, researchers and engineers have explored approaches to guaranteeing a suitable working temperature for LIB, one of which is the battery preheating system. To clarify the advancement of this system, both internal and external preheating methods studied in recent years are summarized, and the discussion for future research is included.
如今,能源和环保问题已成为最受关注的焦点。发展以锂离子电池为动力源的电动汽车是解决这些问题的最有效途径之一。锂离子电池(LIB)因其在能量密度、循环寿命和能量保持方面的突出特点而备受青睐。然而,它对工作温度的严重敏感性导致电动汽车在行驶时出现问题。因此,研究人员和工程师们探索了各种方法来保证 LIB 的工作温度,其中之一就是电池预热系统。为了阐明该系统的进展,本文总结了近年来研究的内部和外部预热方法,并对未来研究进行了讨论。
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引用次数: 0
Performance analysis and application fields of reactive powder concrete 活性粉末混凝土的性能分析和应用领域
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241009
Yuchen Zhao
Reactive Powder Concrete (RPC) is a novel concrete material prepared from highly active composite admixtures, cement, fine sand, and micro steel fibers through appropriate curing and processing techniques. RPC not only exhibits ultra-high strength and durability but also demonstrates outstanding toughness, good volume stability, and excellent environmental performance. Its use can reduce the demand for natural resources, extend the service life of structures, and reduce waste generation, aligning with the direction of sustainable development and green building. This makes it an ideal choice for constructing large-span lightweight structures and structures operating in harsh environmental conditions, attracting extensive research attention in the international engineering materials field. In summary, RPC, as a new type of concrete material, has broad application prospects in the construction field. With continuous research and technological development, RPC will undoubtedly bring more innovations and new solutions to the construction industry, promoting its continuous progress and development.
反应粉末混凝土(RPC)是一种新型混凝土材料,由高活性复合外加剂、水泥、细砂和微钢纤维通过适当的养护和加工技术制备而成。RPC 不仅具有超高的强度和耐久性,还具有出色的韧性、良好的体积稳定性和优异的环保性能。使用它可以减少对自然资源的需求,延长结构的使用寿命,减少废物的产生,符合可持续发展和绿色建筑的方向。因此,它是建造大跨度轻质结构和在恶劣环境条件下运行的结构的理想选择,在国际工程材料领域引起了广泛的研究关注。综上所述,RPC 作为一种新型混凝土材料,在建筑领域有着广阔的应用前景。随着研究的不断深入和技术的不断发展,RPC 无疑将为建筑行业带来更多的创新和新的解决方案,推动建筑行业的不断进步和发展。
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引用次数: 0
Causes and treatment of bridge cracks 桥梁裂缝的原因和处理方法
Pub Date : 2024-05-09 DOI: 10.54254/2755-2721/63/20241019
Zhiyan Zhang
With the large-scale construction and operation of highway bridges in China, the maintenance and repair work of bridges has become increasingly important. The treatment of bridge cracks is a crucial aspect of maintenance and repair. Improper analysis and treatment of cracks in maintenance and repair projects can pose significant safety hazards to bridges. Therefore, it is necessary to conduct a detailed scientific analysis of the cracks in bridges and implement corresponding treatment measures. This paper summarizes common crack diseases in different bridge operation processes, analyzes their causes, and provides relevant references for later maintenance and repair, which has certain reference significance for the maintenance and repair of bridges.
随着中国公路桥梁的大规模建设和运营,桥梁的养护和维修工作变得越来越重要。桥梁裂缝的处理是养护维修的一个重要环节。养护维修工程中对裂缝的分析和处理不当,会给桥梁带来极大的安全隐患。因此,有必要对桥梁裂缝进行详细的科学分析,并实施相应的处理措施。本文总结了不同桥梁运营过程中常见的裂缝病害,分析了其产生的原因,为后期的养护维修提供了相关参考,对桥梁的养护维修具有一定的借鉴意义。
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引用次数: 0
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