Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200015
Rani Iani Costa Gonçalo, Afonso Nóbrega Dantas, Everton Freitas de Morais, Roseana de Almeida Freitas
{"title":"Carcinoma mucoepidermoide em região retromolar: relato de caso","authors":"Rani Iani Costa Gonçalo, Afonso Nóbrega Dantas, Everton Freitas de Morais, Roseana de Almeida Freitas","doi":"10.5935/1676-2444.20200015","DOIUrl":"https://doi.org/10.5935/1676-2444.20200015","url":null,"abstract":"","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"56 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Níveis de antígeno específico da próstata em pacientes com fatores de risco para transtornos do carcinoma da próstata","authors":"Mercedes Terreros, Yolanda Cristina Valdés, Lourdes Beatriz Gonzáles, Onay Adonys Mercader, S. Romero","doi":"10.5935/1676-2444.20200040","DOIUrl":"https://doi.org/10.5935/1676-2444.20200040","url":null,"abstract":"","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"56 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200036
Gian Carlos Ramirez-Ubillus, Carlos Ricardo Neira-Montoya, Eduardo Eulogio Sedano-Gelvet, Joel Fernando Verona-Cueva
{"title":"Validation of a new method for estimating low platelet counts: G&S method","authors":"Gian Carlos Ramirez-Ubillus, Carlos Ricardo Neira-Montoya, Eduardo Eulogio Sedano-Gelvet, Joel Fernando Verona-Cueva","doi":"10.5935/1676-2444.20200036","DOIUrl":"https://doi.org/10.5935/1676-2444.20200036","url":null,"abstract":"","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200050
B. Longo, L. Kuhn, M. Tomasin, Amadeu TOMASIN NETO, Geraldo Grizza, M. Souza
Odontomas are odontogenic tumors subdivided into complex type (common in the posterior mandible region) and compound type. These lesions are usually asymptomatic and diagnosed in routine examinations, are more frequent in the second decade of life. The objective of this case is to report the treatment of a patient with complex odontoma in childhood in the anterior maxilla. A nine-year-old patient sought dental care due to non-eruption of an anterior permanent tooth. After clinical examination and radiographic analysis, the diagnostic hypothesis of odontoma was confirmed. Surgical removal of the lesion and use of orthodontic appliances were indicated. After the therapeutic protocol, the case was completely resolved. Due to the absence of symptoms, many cases of odontomas are neglected. However, it is evident that the sooner diagnosed, the better the prognosis of the patient.
{"title":"Surgical-orthodontic therapy of compound odontoma in the anterior maxilla","authors":"B. Longo, L. Kuhn, M. Tomasin, Amadeu TOMASIN NETO, Geraldo Grizza, M. Souza","doi":"10.5935/1676-2444.20200050","DOIUrl":"https://doi.org/10.5935/1676-2444.20200050","url":null,"abstract":"Odontomas are odontogenic tumors subdivided into complex type (common in the posterior mandible region) and compound type. These lesions are usually asymptomatic and diagnosed in routine examinations, are more frequent in the second decade of life. The objective of this case is to report the treatment of a patient with complex odontoma in childhood in the anterior maxilla. A nine-year-old patient sought dental care due to non-eruption of an anterior permanent tooth. After clinical examination and radiographic analysis, the diagnostic hypothesis of odontoma was confirmed. Surgical removal of the lesion and use of orthodontic appliances were indicated. After the therapeutic protocol, the case was completely resolved. Due to the absence of symptoms, many cases of odontomas are neglected. However, it is evident that the sooner diagnosed, the better the prognosis of the patient.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71117137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200060
Marcel Fruehwirth, A. Rivas, A. F. R. Fitz, A. A. Batista, Cleypson Vinicius Silveira, R. M. Delai
ABSTRACT Introduction: Although reverse transcription-polymerase chain reaction (rRT-PCR) is the gold standard method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), some factors, such as the presence of amplification inhibitors, lead to false-negative results Objective: Here we describe the differences between rRT-PCR results for SARS-CoV-2 infection in normal and diluted samples, simulating the need for dilution due to the presence of amplification inhibitors Material and method: Viral ribonucleic acid (RNA) from samples of nasopharyngeal swabs from 20 patients previously detected as "Negative"and 21 patients detected as "Positive"for SARS-CoV-2 was performed with the EasyExtract DNA-RNA kit (Interprise®) The rRT-PCR was performed with the OneStep/COVID-19 kit (IBMP), with normal and diluted (80 µl of H2O RNAse free) samples, totaling 82 tests Results: The results indicate that there is an average variation (a l0 05) delaying the Cq between the results of amplification of the internal control (IC), N gene (NG), and ORF1ab (OF), 1 811 Cq, 3 840 Cq, and 3 842 Cq, respectively Discussion: The extraction kit does not completely purify the inhibitor compounds;therefore, no amplified product result may occur In this study, we obtained a 19 04% false-negative diagnosis after sample dilution;this process reduces the efficiency of rRT-PCR to 29 8% in detecting SARS-CoV-2 Conclusion: Knowing the rRT-PCR standards of diluted samples can assist in the identification of false-negative cases and, consequently, avoid incorrect diagnosis RESUMEN Introduccion: Aunque la reaccion en cadena de la polimerasa con transcriptasa reversa en tiempo real (rRT-PCR) sea el metodo de referencia para deteccion del coronavirus tipo 2 del sindrome respiratorio agudo grave (Sars-CoV-2), algunos factores como la presencia de inhibidores de amplificacion conducen a resultados falsos negativos Objetivo: Describimos las diferencias entre los resultados de rRT-PCR para infeccion por Sars-CoV-2 en muestras normales y diluidas, simulando la necesidad de dilucion debido a la presencia de inhibidores de amplificacion Material y metodo: La extraccion de acido ribonucleico (ARN) viral de muestras de hisopos nasofaringeos de 20 pacientes previamente detectados como "negativos"y 21 pacientes detectados como "positivos"para Sars-CoV-2 se realizo con el kit Easy Extract DNA-RNA (Interprise®) La rRT-PCR se realizo con el kit OneStep/Covid-19 (IBMP), con muestras normales y diluidas (80 µl de H2O libre de ARNasa), totalizando 82 pruebas Resultados: Los resultados indican que hay una variacion media (a l0,05) retrasando el ciclo de cuantificacion (Cq) entre los resultados de amplificacion del control interno (CI), gen N (GN) y ORF1ab (OF) de 1,811 Cq, 3,840 Cq y 3,842 Cq Discusion: El kit de extraccion no purifica completamente los compuestos inhibidores;por lo tanto, puede ocurrir no amplificacion Obtuvimos un diagnostico falso negativo de 19,04% despues de la diluc
{"title":"False-negative result in molecular diagnosis of SARS-CoV-2 in samples with amplification inhibitors","authors":"Marcel Fruehwirth, A. Rivas, A. F. R. Fitz, A. A. Batista, Cleypson Vinicius Silveira, R. M. Delai","doi":"10.5935/1676-2444.20200060","DOIUrl":"https://doi.org/10.5935/1676-2444.20200060","url":null,"abstract":"ABSTRACT Introduction: Although reverse transcription-polymerase chain reaction (rRT-PCR) is the gold standard method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), some factors, such as the presence of amplification inhibitors, lead to false-negative results Objective: Here we describe the differences between rRT-PCR results for SARS-CoV-2 infection in normal and diluted samples, simulating the need for dilution due to the presence of amplification inhibitors Material and method: Viral ribonucleic acid (RNA) from samples of nasopharyngeal swabs from 20 patients previously detected as \"Negative\"and 21 patients detected as \"Positive\"for SARS-CoV-2 was performed with the EasyExtract DNA-RNA kit (Interprise®) The rRT-PCR was performed with the OneStep/COVID-19 kit (IBMP), with normal and diluted (80 µl of H2O RNAse free) samples, totaling 82 tests Results: The results indicate that there is an average variation (a l0 05) delaying the Cq between the results of amplification of the internal control (IC), N gene (NG), and ORF1ab (OF), 1 811 Cq, 3 840 Cq, and 3 842 Cq, respectively Discussion: The extraction kit does not completely purify the inhibitor compounds;therefore, no amplified product result may occur In this study, we obtained a 19 04% false-negative diagnosis after sample dilution;this process reduces the efficiency of rRT-PCR to 29 8% in detecting SARS-CoV-2 Conclusion: Knowing the rRT-PCR standards of diluted samples can assist in the identification of false-negative cases and, consequently, avoid incorrect diagnosis RESUMEN Introduccion: Aunque la reaccion en cadena de la polimerasa con transcriptasa reversa en tiempo real (rRT-PCR) sea el metodo de referencia para deteccion del coronavirus tipo 2 del sindrome respiratorio agudo grave (Sars-CoV-2), algunos factores como la presencia de inhibidores de amplificacion conducen a resultados falsos negativos Objetivo: Describimos las diferencias entre los resultados de rRT-PCR para infeccion por Sars-CoV-2 en muestras normales y diluidas, simulando la necesidad de dilucion debido a la presencia de inhibidores de amplificacion Material y metodo: La extraccion de acido ribonucleico (ARN) viral de muestras de hisopos nasofaringeos de 20 pacientes previamente detectados como \"negativos\"y 21 pacientes detectados como \"positivos\"para Sars-CoV-2 se realizo con el kit Easy Extract DNA-RNA (Interprise®) La rRT-PCR se realizo con el kit OneStep/Covid-19 (IBMP), con muestras normales y diluidas (80 µl de H2O libre de ARNasa), totalizando 82 pruebas Resultados: Los resultados indican que hay una variacion media (a l0,05) retrasando el ciclo de cuantificacion (Cq) entre los resultados de amplificacion del control interno (CI), gen N (GN) y ORF1ab (OF) de 1,811 Cq, 3,840 Cq y 3,842 Cq Discusion: El kit de extraccion no purifica completamente los compuestos inhibidores;por lo tanto, puede ocurrir no amplificacion Obtuvimos un diagnostico falso negativo de 19,04% despues de la diluc","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71117261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200025
B. F. B. B. Abreu, M. Duarte, M. Grande, N. M. Lima Neto, Edimeire F. Ottoni
A 45-year-old man presents with occipital headache for about one month, resistant to analgesic therapy, and palpable nodule at the pain site. At initial evaluation, the nodule was 1.5 cm in diameter without apparent skin alteration. Ultrasonography revealed a well-defined osteolytic lesion and doubtful communication with the dura mater. The study was complemented with computed tomography and contrast-enhanced magnetic resonance, both identifying lytic lesion with soft tissue component and contrast enhancement, without diffusion restriction, guiding the diagnostic hypothesis of eosinophilic granuloma. Surgical treatment with mass excision and anatomopathological evaluation was made, which confirmed the radiological diagnostic hypothesis. After three months of follow-up, the patient remained symptom-free and radiographic control showed no signs of relapse.
{"title":"Eosinophilic granuloma: an unusual finding in adults","authors":"B. F. B. B. Abreu, M. Duarte, M. Grande, N. M. Lima Neto, Edimeire F. Ottoni","doi":"10.5935/1676-2444.20200025","DOIUrl":"https://doi.org/10.5935/1676-2444.20200025","url":null,"abstract":"A 45-year-old man presents with occipital headache for about one month, resistant to analgesic therapy, and palpable nodule at the pain site. At initial evaluation, the nodule was 1.5 cm in diameter without apparent skin alteration. Ultrasonography revealed a well-defined osteolytic lesion and doubtful communication with the dura mater. The study was complemented with computed tomography and contrast-enhanced magnetic resonance, both identifying lytic lesion with soft tissue component and contrast enhancement, without diffusion restriction, guiding the diagnostic hypothesis of eosinophilic granuloma. Surgical treatment with mass excision and anatomopathological evaluation was made, which confirmed the radiological diagnostic hypothesis. After three months of follow-up, the patient remained symptom-free and radiographic control showed no signs of relapse.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200008
M. Duarte, É. R. Duarte, Edgard Brasil Solorzano, J. Ferreira
A 41-year-old male patient refers to a painful tumor on the lateral side of the right leg. Ultrasonography visualized a predominantly hypoechoic well-delineated large heterogeneous mass in the subcutaneous tissue, showing an internal hyperechoic area. Magnetic resonance imaging (MRI) demonstrated an oval and lobulated tumor with well-defined margins at the anterolateral part of the leg, with contrast enhancement. The anatomopathological evaluation revealed a neoplasm with an extensive chondromyxoid matrix. The immunohistochemical study demonstrated positive vimentin (V9 clone), positive S-100 protein and negative desmin (D33 clone), smooth muscle actin (1A4), CD68 (KP1 clone) and caldesmon. The set of findings is compatible with myxoid liposarcoma.
{"title":"Leg myxoid liposarcoma: correlation between image and anatomopathological\u0000 tests","authors":"M. Duarte, É. R. Duarte, Edgard Brasil Solorzano, J. Ferreira","doi":"10.5935/1676-2444.20200008","DOIUrl":"https://doi.org/10.5935/1676-2444.20200008","url":null,"abstract":"A 41-year-old male patient refers to a painful tumor on the lateral side of the right leg. Ultrasonography visualized a predominantly hypoechoic well-delineated large heterogeneous mass in the subcutaneous tissue, showing an internal hyperechoic area. Magnetic resonance imaging (MRI) demonstrated an oval and lobulated tumor with well-defined margins at the anterolateral part of the leg, with contrast enhancement. The anatomopathological evaluation revealed a neoplasm with an extensive chondromyxoid matrix. The immunohistochemical study demonstrated positive vimentin (V9 clone), positive S-100 protein and negative desmin (D33 clone), smooth muscle actin (1A4), CD68 (KP1 clone) and caldesmon. The set of findings is compatible with myxoid liposarcoma.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"56 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200029
Rafael de Lima Miguel, Luiz Claudio Miletti, Bruna Fernanda da Silva
Introduction: Chlamydia trachomatis is one of the major causative agents of sexually transmitted infections (STI) in men and women, but the greatest impact of infection occurs in the reproductive system of women, causing, among other problems, infertility. Objective: To estimate the incidence of C. trachomatis detected by the polymerase chain reaction (PCR) technique in endocervical samples from women in Lages, Santa Catarina, Brazil. Methods: A descriptive, quantitative, cross-sectional study was conducted from May to November 2017. Endocervical cell samples were collected from 126 women that receive medical assistance at three primary healthcare units and one gynecology clinic in the city. The samples of endocervical material were evaluated for the diagnosis of C. trachomatis by the PCR technique. Prior to sample collection, a Cancer Information System (SISCAN) standard questionnaire was answered by the participants. Fisher’s exact test and chi-square test were used to evaluate the association between infection and the variables of the questionnaire. Results: From the 126 endocervical samples analyzed, 39.7% were positive for C. trachomatis. The average age of the participants was 31.7 years old. From the 118 women who did not show changes in the cervix, 43 were positive for C. trachomatis, which reinforces the asymptomatic character of the infection. There was no significant association between endocervical infection by C. trachomatis and age, use of contraceptive pill, time of last pap test and signs of STI. Conclusion: The incidence of C. trachomatis infection in the endocervical samples from these women can be considered high, which emphasizes the importance of measures designed to clarify and prevent this important STI.
{"title":"Incidence of Chlamydia trachomatis detected by PCR in women’s\u0000 endocervical samples in Lages, Santa Catarina, Brazil","authors":"Rafael de Lima Miguel, Luiz Claudio Miletti, Bruna Fernanda da Silva","doi":"10.5935/1676-2444.20200029","DOIUrl":"https://doi.org/10.5935/1676-2444.20200029","url":null,"abstract":"Introduction: Chlamydia trachomatis is one of the major causative agents of sexually transmitted infections (STI) in men and women, but the greatest impact of infection occurs in the reproductive system of women, causing, among other problems, infertility. Objective: To estimate the incidence of C. trachomatis detected by the polymerase chain reaction (PCR) technique in endocervical samples from women in Lages, Santa Catarina, Brazil. Methods: A descriptive, quantitative, cross-sectional study was conducted from May to November 2017. Endocervical cell samples were collected from 126 women that receive medical assistance at three primary healthcare units and one gynecology clinic in the city. The samples of endocervical material were evaluated for the diagnosis of C. trachomatis by the PCR technique. Prior to sample collection, a Cancer Information System (SISCAN) standard questionnaire was answered by the participants. Fisher’s exact test and chi-square test were used to evaluate the association between infection and the variables of the questionnaire. Results: From the 126 endocervical samples analyzed, 39.7% were positive for C. trachomatis. The average age of the participants was 31.7 years old. From the 118 women who did not show changes in the cervix, 43 were positive for C. trachomatis, which reinforces the asymptomatic character of the infection. There was no significant association between endocervical infection by C. trachomatis and age, use of contraceptive pill, time of last pap test and signs of STI. Conclusion: The incidence of C. trachomatis infection in the endocervical samples from these women can be considered high, which emphasizes the importance of measures designed to clarify and prevent this important STI.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"56 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200031
Ângelo M. Rolim, Felipe Borges, A. Barros, Jonathan D. Lima, Fabiana B. A. Silva, Hélia Carla de Souza, D. Freire, L. Ferreira, Izabel Cristina R. Silva
Interleukin-4 (IL-4) has great significance in inflammatory processes in cases of stroke, since it is able to polarize microglia to the anti-inflammatory phenotype called M2. This study analyzed if the variation between TT genotype and the other genotypes (CT and CC), in -589 (rs2243250) polymorphism of IL4 gene, has association with the prognosis of hemorrhagic stroke (HS) and with clinical aspects which are risk factors for cerebrovascular diseases. The result of this study shows that there is no statistical association of the IL4 polymorphism with either prognosis or clinical aspects in HS patients.
{"title":"Statistical association of rs2243250 polymorphism of IL4 gene and hemorrhagic stroke in Brazilian population","authors":"Ângelo M. Rolim, Felipe Borges, A. Barros, Jonathan D. Lima, Fabiana B. A. Silva, Hélia Carla de Souza, D. Freire, L. Ferreira, Izabel Cristina R. Silva","doi":"10.5935/1676-2444.20200031","DOIUrl":"https://doi.org/10.5935/1676-2444.20200031","url":null,"abstract":"Interleukin-4 (IL-4) has great significance in inflammatory processes in cases of stroke, since it is able to polarize microglia to the anti-inflammatory phenotype called M2. This study analyzed if the variation between TT genotype and the other genotypes (CT and CC), in -589 (rs2243250) polymorphism of IL4 gene, has association with the prognosis of hemorrhagic stroke (HS) and with clinical aspects which are risk factors for cerebrovascular diseases. The result of this study shows that there is no statistical association of the IL4 polymorphism with either prognosis or clinical aspects in HS patients.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5935/1676-2444.20200027
Roberta Kelly L. Souza, Etienne W. Coan, M. I. Anghebem
Introduction: The nonconformities detected in the pre-analytical phase of laboratory tests sent to the Central Public Health Laboratories (Lacen) culminate in the loss of epidemiological data of great importance for public health and are detrimental to health surveillance. This study aimed to identify the most frequent pre-analytical nonconformities recorded by Lacen/PR and the major difficulties encountered by the primary and regional health units in the registration of exams in the Laboratory Environment Manager (GAL) system. Results: The analysis of data from the Paraná GAL system in 2017 identified 9,723 discards for disagreeing samples in a total of 132,567 tests performed in the same period. The most frequent nonconformities were: request canceled by the GAL management due to expiration of the screening period (28%), and sample unsuitable for the requested analysis (28%). Discussion: After identifying the vulnerabilities of this stage of the process, the greatest detected difficulty was requesting the correct test. Conclusion: Data indicate the need to strengthen training and improve the pre-analytical process in order to ensure patient safety and epidemiological data.
{"title":"Nonconformities in the pre-analytical phase identified in a public health laboratory","authors":"Roberta Kelly L. Souza, Etienne W. Coan, M. I. Anghebem","doi":"10.5935/1676-2444.20200027","DOIUrl":"https://doi.org/10.5935/1676-2444.20200027","url":null,"abstract":"Introduction: The nonconformities detected in the pre-analytical phase of laboratory tests sent to the Central Public Health Laboratories (Lacen) culminate in the loss of epidemiological data of great importance for public health and are detrimental to health surveillance. This study aimed to identify the most frequent pre-analytical nonconformities recorded by Lacen/PR and the major difficulties encountered by the primary and regional health units in the registration of exams in the Laboratory Environment Manager (GAL) system. Results: The analysis of data from the Paraná GAL system in 2017 identified 9,723 discards for disagreeing samples in a total of 132,567 tests performed in the same period. The most frequent nonconformities were: request canceled by the GAL management due to expiration of the screening period (28%), and sample unsuitable for the requested analysis (28%). Discussion: After identifying the vulnerabilities of this stage of the process, the greatest detected difficulty was requesting the correct test. Conclusion: Data indicate the need to strengthen training and improve the pre-analytical process in order to ensure patient safety and epidemiological data.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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