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Comparison of Morphological Analysis and DNA Metabarcoding of Crustacean Mesozooplankton in the Yellow Sea 黄海甲壳纲中浮游动物形态分析与DNA元条形码比较
Q4 Engineering Pub Date : 2021-03-01 DOI: 10.4217/OPR.2021.43.1.045
Garam Kim, Hyung-Ku Kang, Choong-gon Kim, Jae Ho Choi, S. Kim
Studies on marine zooplankton diversity and ecology are important for understanding marine ecosystem, as well as environmental conservation and fisheries management. DNA metabarcoding is known as a useful tool to reveal and understand diversity among animals, but a comparative evaluation with classical microscopy is still required in order to properly use it for marine zooplankton research. This study compared crustacean mesozooplankton taxa revealed by morphological analysis and metabarcoding of the cytochrome oxidase I (COI). A total of 17 crustacean species were identified by morphological analysis, and 18 species by metabarcoding. Copepods made up the highest proportion of taxa, accounting for more than 50% of the total number of species delineated by both methods. Cladocerans were not found by morphological analysis, whereas amphipods and mysids were not detected by metabarcoding. Unlike morphological analysis, metabarcoding was able to identify decapods down to the species level. There were some discrepancies in copepod species, which could be due to a lack of genetic database, or biases during DNA extraction, amplification, pooling and bioinformatics. Morphological analysis will be useful for ecological studies as it can classify and quantify the life history stages of marine zooplankton that metabarcoding cannot detect. Metabarcoding can be a powerful tool for determining marine zooplankton diversity, if its methods or database are further supplemented.
对海洋浮游动物多样性和生态学的研究对于理解海洋生态系统、环境保护和渔业管理具有重要意义。众所周知,DNA代谢编码是揭示和理解动物多样性的有用工具,但为了将其正确用于海洋浮游动物研究,仍需要与经典显微镜进行比较评估。本研究比较了通过细胞色素氧化酶I(COI)的形态学分析和代谢编码揭示的甲壳类动物-中型浮游动物分类群。通过形态学分析共鉴定出17种甲壳类动物,通过代谢条形码鉴定出18种。桡足类在分类群中所占比例最高,占两种方法所划分物种总数的50%以上。通过形态学分析没有发现枝角类,而通过代谢编码没有检测到片脚类和mysides。与形态学分析不同,代谢条形码能够识别下至物种水平的十足类动物。桡足类物种存在一些差异,这可能是由于缺乏基因数据库,或者在DNA提取、扩增、汇集和生物信息学过程中存在偏差。形态学分析将有助于生态学研究,因为它可以对代谢条形码无法检测到的海洋浮游动物的生活史阶段进行分类和量化。如果进一步补充其方法或数据库,代谢编码可以成为确定海洋浮游动物多样性的有力工具。
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引用次数: 0
Estimation of Extreme Wind Speeds in the Western North Pacific Using Reanalysis Data Synthesized with Empirical Typhoon Vortex Model 利用经验台风涡旋模式合成的再分析资料估算西北太平洋极端风速
Q4 Engineering Pub Date : 2021-03-01 DOI: 10.4217/OPR.2021.43.1.001
Hyejin Moon
In this study, extreme wind speeds in the Western North Pacific (WNP) were estimated using reanalysis wind fields synthesized with an empirical typhoon vortex model. Reanalysis wind data used is the Fifth-generation European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis (ERA5) data, which was deemed to be the most suitable for extreme value analysis in this study. The empirical typhoon vortex model used has the advantage of being able to realistically reproduce the asymmetric winds of a typhoon by using the gale/storm-forced wind radii information in the 4 quadrants of a typhoon. Using a total of 39 years of the synthesized reanalysis wind fields in the WNP, extreme value analysis is applied to the General Pareto Distribution (GPD) model based on the Peak-Over-Threshold (POT) method, which can be used effectively in case of insufficient data. The results showed that the extreme analysis using the synthesized wind data significantly improved the tendency to underestimate the extreme wind speeds compared to using only reanalysis wind data. Considering the difficulty of obtaining long-term observational wind data at sea, the result of the synthesized wind field and extreme value analysis developed in this study can be used as basic data for the design of offshore structures.
在这项研究中,北太平洋西部(WNP)的极端风速是使用再分析风场和经验台风涡旋模型综合估计的。使用的再分析风数据是第五代欧洲中期天气预报中心(ECMWF)再分析(ERA5)数据,该数据被认为是本研究中最适合进行极值分析的数据。所使用的经验台风涡旋模型的优点在于,通过使用台风的4个象限中的大风/风暴强迫风半径信息,能够真实地再现台风的不对称风。利用WNP 39年的综合再分析风场,将极值分析应用于基于峰值过阈值(POT)方法的广义帕累托分布(GPD)模型,在数据不足的情况下可以有效地使用该方法。结果表明,与仅使用再分析风数据相比,使用合成风数据的极端分析显著改善了低估极端风速的趋势。考虑到获取海上长期观测风数据的困难,本研究开发的综合风场和极值分析结果可作为海上结构物设计的基础数据。
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引用次数: 1
Correlation Analysis Between the Variation of Net Surface Heat Flux Around the East Asian Seas and the Air Temperature and Precipitation Over the Korean Peninsula 东亚海域海面净热通量变化与朝鲜半岛气温和降水的相关性分析
Q4 Engineering Pub Date : 2021-03-01 DOI: 10.4217/OPR.2021.43.1.015
Seokheung Chang
In this study, using 16 ORA-IP (Ocean Reanalysis Intercomparison Project) data, we investigated spatial and temporal changes of net surface heat flux in the East Asian seas and presented a new ensemble net surface heat flux index. The ensemble net surface heat flux index is produced considering the data distribution and the standard deviation of each ORA-IP. From the correlation analysis with air temperature averaged over the Korean Peninsula, ensemble net heat flux around the Korea Strait shows the highest correlation (0.731) with a 3 month time lag. For the correlation study regarding precipitation over the Korean Peninsula, it also shows significant correlation especially in winter and spring seasons. Similar results are also found in comparison with climate indices (AO, PDO, and NINO3.4), but ensemble net surface heat flux data in winter season reveals the strongest correlation patterns especially with winter temperature and spring precipitation.
本研究利用16个ORA-IP(海洋再分析相互比较项目)数据,研究了东亚海域净表面热通量的时空变化,并提出了一个新的综合净表面热流量指数。综合净表面热通量指数是在考虑数据分布和每个ORA-IP的标准偏差的情况下产生的。根据与朝鲜半岛平均气温的相关性分析,朝鲜海峡周围的整体净热通量显示出最高的相关性(0.731),具有3个月的时滞。对于朝鲜半岛上空降水的相关性研究,它也显示出显著的相关性,尤其是在冬季和春季。与气候指数(AO、PDO和NINO3.4)相比,也发现了类似的结果,但冬季的整体净表面热通量数据显示出最强的相关性模式,尤其是与冬季温度和春季降水的相关性。
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引用次数: 0
Weekly Variation of Prokaryotic Growth and Diversity in the Inner Bay of Yeong-do, Busan 釜山杨岛内湾原核生物生长和多样性的周变化
Q4 Engineering Pub Date : 2021-03-01 DOI: 10.4217/OPR.2021.43.1.031
Wonseok Yang, J. Noh, Howon Lee, Yeonjung Lee, D. Choi
To understand the temporal variation of prokaryotic communities in a temperate coastal area, prokaryotic abundance, activity, and community composition were investigated every week for over a year at a coastal monitoring station of Yeong-do, Busan. The prokaryotic abundances fluctuated about 10 times, ranging from 2.0 to 20.1 × 105 cells mL-1 and tended to be high in spring when phytoplankton bloom occurred. The prokaryotic thymidine incorporation rates (TTI) varied in a low range between 0.2 and 11.5 pmol L-1 h-1 in winter. However, in summer, TTI were increased up to a range of 8.3 to 17.4 pmol L-1 h-1, showing an increasing pattern in summer. During the study period, Alphaproteobacteria was the most dominant class for most of the year, followed by Flavobacteria. While the seasonal variation of prokaryotic composition was not apparent at the class level, many prokaryotic species showed a distinct temporal or seasonal variation for the year. In the coastal site, prokaryotic biomass and activity did not show significant correlations with temperature and chlorophyll-a, which are well known to regulate prokaryotic growth in marine environments, suggesting that the study area may be affected by diverse sources of organic matter for their growth.
为了了解温带沿海地区原核生物群落的时间变化,在釜山杨岛的一个沿海监测站,一年多来每周都对原核生物的丰度、活性和群落组成进行调查。原核丰度波动约10倍,范围为2.0至20.1×105个细胞mL-1,在春季浮游植物爆发时趋于较高。在冬季,原核胸苷掺入率(TTI)在0.2至11.5pmol L-1 h-1之间的低范围内变化。然而,在夏季,TTI增加到8.3至17.4pmol L-1 h-1的范围,显示出夏季的增加模式。在研究期间,在一年的大部分时间里,α变形菌是最具优势的类群,其次是黄细菌。虽然原核生物组成的季节变化在类水平上并不明显,但许多原核生物物种在一年中表现出明显的时间或季节变化。在沿海地区,原核生物量和活性与温度和叶绿素a没有显示出显著的相关性,众所周知,叶绿素a可以调节原核在海洋环境中的生长,这表明研究区域可能受到不同有机物来源的影响。
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引用次数: 2
Mesozooplankton Community Structure in the Yellow Sea in Spring 黄海春季中浮游动物群落结构
Q4 Engineering Pub Date : 2020-12-01 DOI: 10.4217/OPR.2020.42.4.271
Garam Kim, Hyung-Ku Kang
We investigated mesozooplankton in the Yellow Sea in spring to understand its community structure and relationship with environmental factors. Total mesozooplankton density ranged from 1,542 to 7,367 ind. m and the biomass ranged from 3 to 42 mg C m. The total density and biomass had a positive relationship with chlorophyll-a (chl-a) concentration. The mesozooplankton community was divided into two groups at 125.5 E by cluster analysis: one was an inshore group and the other was an offshore group. The inshore group of mesozooplankton was of high density but low diversity, while the offshore group was of high diversity but low density. Copepod Acartia hongi and its copepodites were the most abundant species, comprising 27.8% of the total mesozooplankton density. A. hongi was especially abundant at the inshore, serving as the indicator species of the inshore group. Redundancy analysis found a positive relationship between the density of A. hongi and chl-a concentration. Oithona similis and Centropages abdominalis were 2 and 3 dominant species comprising 9 and 7% of the total density, respectively. The density of O. similis was positively related to water depth, but C. abdominalis was related to chl-a concentration. Chl-a concentration seems to influence significantly the mesozooplankton community structure in the Yellow Sea in spring, rather than water temperature or salinity.
为了解黄海春季浮游动物群落结构及其与环境因子的关系,对黄海春季浮游动物进行了调查。浮游动物总密度在1542 ~ 7367 ind. m之间,生物量在3 ~ 42 mg C . m之间,总密度和生物量与叶绿素a (chl-a)浓度呈正相关。通过聚类分析,在125.5 E时将中浮游动物群落划分为近岸群和近海群。中浮游动物的近岸类群密度高,多样性低,近海类群多样性高,密度低。其中桡足类红足类及其桡足类数量最多,占总浮游动物密度的27.8%。红背刺槐在近岸的分布尤其丰富,是近岸类群的指示种。冗余分析发现,红木密度与chl-a浓度呈正相关。大腹棘蝗(Oithona similis)和腹腹棘蝗(Centropages abdominalis)为优势种,分别占总密度的9%和7%。相似鲈密度与水深呈正相关,腹鲈密度与chl-a浓度呈正相关。春季黄海中浮游动物群落结构受Chl-a浓度的显著影响,而水温和盐度的影响较小。
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引用次数: 1
New Record of Oncaea prendeli (Copepod, Cyclopoida, Oncaeidae) in Korean Waters 新纪录的Oncaea prendeli(桡足动物、剑水蚤目Oncaeidae)在韩国水域
Q4 Engineering Pub Date : 2020-12-01 DOI: 10.4217/OPR.2020.42.4.283
Kyuhee Cho, Jong Guk Kim, Jimin Lee
Oncaea prendeli Shmeleva, 1966 is redescribed here based on a specimen collected using a fine-mesh net in the epipelagic layer in the south sea of Jeju Island, Korea. The Korean specimen is generally similar to Mediterranean specimens in terms of morphological characteristics, but it shows a few minor differences in a slightly larger length-to-width ratio for the distal endopod segment of the antenna, a relatively shorter caudal seta IV, and additional ornamentation on the maxillule and maxilla. This species is distinguishable from the most similar congeneric species, O. tregoubovi Shmeleva, 1968, and three species of the genus Spinoncaea Böttger-Schnack, 2003 by the distal and lateral armature of the antenna, ornamentation on the female maxilliped, spine number on the endopod of leg 2, and proportional length of setae on the caudal ramus. Moreover, spine lengths on the exopods of legs 2–4 are proposed as new morphological characters for the identification of congeners. This is the first record of O. prendeli in Korean waters.
本文根据在韩国济州岛南海的上层海底用细网网采集的标本,对1966年的Oncaea prendeli Shmeleva进行了重新描述。朝鲜标本在形态特征上与地中海标本大体相似,但在触角远端内足节的长宽比略大、尾端第四节相对较短、上颌骨和上颌骨上有额外的纹饰等方面表现出一些细微的差异。该种与最相似的同属种O. tregoubovi Shmeleva(1968)和Spinoncaea Böttger-Schnack(2003)的三个种的区别是:触角的远端和外侧枢枢、雌性上唇的纹饰、腿2的内足上的棘数和尾支上刚毛的比例长度。此外,还提出了腿2-4的外足上的脊柱长度作为同系物鉴定的新形态特征。这是在韩国海域首次记录到O. prendeli。
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引用次数: 2
Study on Load Reduction of a Tidal Steam Turbine Using a Flapped Blade 潮汐式汽轮机襟翼减载研究
Q4 Engineering Pub Date : 2020-12-01 DOI: 10.4217/OPR.2020.42.4.293
Dasom Jeong
Blades of tidal stream turbines have to sustain many different loads during operation in the underwater environment, so securing their structural safety is a key issue. In this study, we focused on periodic loads due to wave orbital motion and propose a load reduction method with a blade design. The flap of an airplane wing is a well-known structure designed to increase lift, and it can also change the load distribution on the wing through deflection. For this reason, we adopted a passive flap structure for the load reduction and investigated its effectiveness by an analytical method based on the blade element moment theory. Flap torsional stiffness required for the design of the passive flap can be obtained by calculating the flap moment based on the analytic method. Comparison between a flapped and a fixed blade showed the effect of the flap on load reduction in a high amplitude wave condition.
潮汐流涡轮机的叶片在水下环境中运行时必须承受许多不同的载荷,因此确保其结构安全是一个关键问题。在这项研究中,我们专注于波浪轨道运动引起的周期性载荷,并提出了一种叶片设计的减载方法。机翼的襟翼是一种众所周知的结构,旨在增加升力,它还可以通过偏转改变机翼上的载荷分布。为此,我们采用了一种被动襟翼结构来减轻载荷,并基于叶片单元力矩理论的分析方法研究了其有效性。被动襟翼设计所需的襟翼扭转刚度可以通过基于解析法计算襟翼力矩来获得。襟翼和固定叶片之间的比较表明,在高振幅波浪条件下,襟翼对减载的影响。
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引用次数: 0
Embryonic Development and Hatchability in the Reciprocal Hybrids of Kelp Grouper (Epinephelus bruneus) and Red-Spotted Grouper (Epinephelus akaara) 褐带石斑鱼(Epinephelus bruneus)和赤斑石斑鱼(Epinephelus akaara)互交杂种的胚胎发育和孵化率
Q4 Engineering Pub Date : 2020-12-01 DOI: 10.4217/OPR.2020.42.4.303
M. Kang, C. Noh, H. Choi, Dae Won Park, Sung-Pyo Hur
In this study, the embryonic development and hatchability of eggs fertilized by the reciprocal hybrids of kelp grouper (Epinephelus bruneus) and red-spotted grouper (E. akaara) were evaluated with the goal of establishing a novel hybrid with enhanced growth and viability during the farming period in the temperate waters of Korea. The fertilization rates were lower for hybrids than for maternal purebreds and were significantly higher in the red-spotted grouper ♀ × kelp grouper ♂ hybrid (RGKG, 89.61 ± 1.58%) than in the kelp grouper ♀ × red-spotted grouper ♂ hybrid (KGRG, 74.82 ± 4.23%, p < 0.05). Unlike the fertilization rates, the hatching rates of fertilized eggs were similar between hybrids and maternal purebreds and did not differ significantly between KGRG and RGKG (72.74 ± 3.60% vs. 75.23 ± 2.20%, respectively, p > 0.05). The embryonic development of the hybrids was similar to that of maternal purebreds; however, irregular cleavage and asymmetric blastoderm were noticeable in the developing eggs of KGRG hybrids. The deformity rates of newly hatched larvae were higher in hybrids than in maternal purebreds and were significantly higher in KGRG than in RGKG (17.47 ± 1.28% vs. 7.11 ± 0.54%, respectively, p < 0.05). These results demonstrate the potential to produce viable larvae from these two hybrids. Although the production efficiency of KGRG was lower than that of RGKG, the fertilization, hatching, and deformity rates make both hybrids useful for further comparative studies regarding economic aspects.
在本研究中,为了在韩国温带水域养殖期间建立一种生长和生存能力增强的新型杂交鱼种,研究了带石斑鱼(Epinephelus bruneus)和红斑石斑鱼(E. akaara)的互交杂交鱼卵的胚胎发育和孵化能力。赤斑石斑鱼♀×赤斑石斑鱼♂杂交的受精率(RGKG, 89.61±1.58%)显著高于赤斑石斑鱼♀×赤斑石斑鱼♂杂交(KGRG, 74.82±4.23%,p < 0.05)。与受精率不同,杂种与母系纯种的受精卵孵化率相似,KGRG与RGKG的受精卵孵化率差异不显著(分别为72.74±3.60%和75.23±2.20%,p < 0.05)。杂种的胚胎发育与母系纯种相似;然而,KGRG杂交种的卵裂不规则,胚皮不对称。杂交种的新孵化仔鱼畸形率高于母系纯种,KGRG组的畸形率显著高于RGKG组(17.47±1.28%比7.11±0.54%,p < 0.05)。这些结果表明,这两种杂交品种有可能产生可活的幼虫。虽然KGRG的生产效率低于RGKG,但在受精率、孵化率和畸形率方面,这两个杂交种在经济方面有进一步的比较研究价值。
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引用次数: 0
The Influence of the Dissolved Oxygen of Bottom Water on the Temporal Variation of the Benthic Polychaetous Community Structure in Dangdong Bay 底层水体溶解氧对当东湾底栖多毛动物群落结构时间变化的影响
Q4 Engineering Pub Date : 2020-09-01 DOI: 10.4217/OPR.2020.42.3.233
Jin Yang, H. Shin
This study was carried out at 5 sites 11 times over two years to identify the variation of benthic environments and benthic polychaetous community and analyze the benthic healthiness in Dangdong Bay, a small semi-enclosed inner bay of Jinhae Bay. The temperature of bottom water showed the typical temporal fluctuation of a temperate zone and was in the range of 5.94 ~ 23.94°C. The salinity did not change significantly during the study period and was in the range of 32.93 ~ 35.72 psu. The concentration of dissolved oxygen of bottom water fluctuated a great deal and was in the range of 0.31 ~ 10.20 mg/L. The lowest DO value was recorded in July 2015, as 0.31±0.04 mg/L corresponding to the hypoxic water mass. The hypoxic water mass was formed continuously at some sites also in July and August 2016. The mean grain size was in the range of 7.57 ~ 9.81Ø and the average was 8.89±0.20Ø. The surface sediments were mainly composed of fine sediment (mud) above 85%. The mean of TOC was 3.09±0.22% and LOI was 13.30±0.47%, showing very high levels in Korean coastal waters. The concentration of AVS was in the range of 0.33 ~ 1.28 mgS/g-dry. The high values of organic contents and AVS indicated that there had been the serious organic enrichment in Dangdong Bay. The number of species and the density of the benthic polychaetous community in Dangdong Bay were in the range of 2 ~ 38 species and 2 ~ 2,185 ind./m2 during the study period. The number of species and density were highly sustained in winter and spring, and then decreased gradually with the formation of a hypoxic water mass in summer, and the lowest number of species and density were recorded in autumn. In September and November 2015, the dead zone expanded to almost the whole study area. Dominant polychaetous species were Capitella capitata, Lumbrineris longifolia, Paraprionospio patiens and Sigambra tentaculata, each known as opportunistic species and potential organic pollutant indicator species. In particular, Paraprionospio patiens showed a very high population density of 2,019 ind./m2 in December 2016. Polychaetous communities at each sampling time were classified into 4 temporal groups according to dominant species in each period by cluster analysis and nMDS. ‘Period Group AI’ was formed in winter and spring of 2015, dominated by Capitella capitata, ‘Period AII’in summer dominated by Lumbrineris longifolia, ‘Period B’ in autumn with no fauna in the dead zone, and particularly‘Period C’ in winter of 2016 dominated by Paraprionospio patiens. As a result of analysis of benthic healthiness, the study area was estimated to be in a Fair~Very Poor condition by AMBI *Corresponding author. E-mail: shinhc@jnu.ac.kr 234 Kim, S. H. et al.
本研究在近两年的时间里,在5个地点进行了11次,以确定金海湾半封闭内湾党东湾的海底环境和海底多毛类群落的变化,并分析其海底健康状况。底层水温呈现典型的温带时间波动,在5.94~23.94°C之间。研究期间盐度变化不大,在32.93~35.72 psu之间。底层水中溶解氧浓度波动较大,在0.31~10.20mg/L之间。DO最低值记录在2015年7月,为0.31±0.04 mg/L,对应于缺氧水团。缺氧水团在2016年7月和8月也在一些部位连续形成。平均粒径在7.57~9.81Å之间,平均粒径为8.89±0.20Å。表层沉积物主要由85%以上的细沉积物(泥)组成。TOC平均值为3.09±0.22%,LOI平均值为13.30±0.47%,显示韩国沿海水域的TOC水平非常高。AVS的浓度范围为0.33~1.28mgS/g-dry。有机质含量和AVS值较高,表明党东湾有机质富集严重。研究期间,党东湾底栖多毛类群落的数量和密度在2~38种和2~2185 ind./m2之间。物种数量和密度在冬季和春季高度持续,然后在夏季随着缺氧水团的形成而逐渐减少,秋季物种数量和浓度最低。2015年9月和11月,死区几乎扩大到整个研究区域。优势多毛类为Capitella capita、Lubrineris longifolia、Paraprionospio patients和Sigambra tentaculata,分别被称为机会性物种和潜在有机污染物指示物种。特别是,2016年12月,Paraprionospio患者的人口密度非常高,达到2019 ind./m2。通过聚类分析和nMDS,将每个采样时间的多血细胞群落按每个时期的优势种分为4个时间组时期群AI’形成于2015年冬春季,以Capitella capitata为主,夏季以Lubrineris longifolia为主,秋季以B期为主,死区内无动物群,特别是2016年冬季以Paraprionospio patients为主的C期。根据对底栖生物健康状况的分析,AMBI*通讯作者估计该研究区域的状况一般至极差。电子邮件:shinhc@jnu.ac.kr234 Kim,S.H.等人。
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引用次数: 0
Characteristics and Paleoceanographic Implications of Grain-size Distributions of Biogenic Components in Sediments from the South Korea Plateau (East Sea) 韩国高原(东海)沉积物中生物组分粒度分布特征及其古海洋学意义
Q4 Engineering Pub Date : 2020-09-01 DOI: 10.4217/OPR.2020.42.3.249
Jungseok Jang, J. Bahk, Eun-jung Kim, I. Um
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引用次数: 1
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Ocean and Polar Research
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