ABSTRACT The analgesic and anti-inflammatory effects of Grewia asiatica leaf solvent extracts were evaluated in animal models. The methanol and ethyl acetate fractions prepared from the leaves showed potent analgesic and anti-inflammatory effects at 400 mg kg−1 and 100 mg kg−1, respectively. Myricetin was isolated from the ethyl acetate fraction by column chromatography. Molecular docking studies with myricetin showed a desirable binding pattern of myricetin with both COX-1 and COX-2, supporting its anti-inflammatory potential. Results showed that the anti-inflammatory and analgesic effect of methanol extract of G. asiatica leaves may be due to COX-1 and COX-2 inhibition by myricetin.
{"title":"Ameliorative Effects of Grewia asiatica Leaves in Animal Models of Pain and Inflammation","authors":"Atamjit Singh, Harpreet Kaur, Hasandeep Singh, Balbir Singh, P. Bedi, Sarabjit Kaur","doi":"10.1080/10496475.2022.2089429","DOIUrl":"https://doi.org/10.1080/10496475.2022.2089429","url":null,"abstract":"ABSTRACT The analgesic and anti-inflammatory effects of Grewia asiatica leaf solvent extracts were evaluated in animal models. The methanol and ethyl acetate fractions prepared from the leaves showed potent analgesic and anti-inflammatory effects at 400 mg kg−1 and 100 mg kg−1, respectively. Myricetin was isolated from the ethyl acetate fraction by column chromatography. Molecular docking studies with myricetin showed a desirable binding pattern of myricetin with both COX-1 and COX-2, supporting its anti-inflammatory potential. Results showed that the anti-inflammatory and analgesic effect of methanol extract of G. asiatica leaves may be due to COX-1 and COX-2 inhibition by myricetin.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47773546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-16DOI: 10.1080/10496475.2022.2084804
T. S. Moe, M. Thida, H. Win, Khine Zar Wynn Myint, Zar Kyi Win, Nwe Nwe Htay
ABSTRACT Antidiabetic and cytotoxic properties of selected indigenous Myanmar medicinal plants were assessed. In vitro scavenging assays of 2,2-diphenyl-1- picrylhydrazyl, nitric oxide, superoxide radicals, and ferric reducing antioxidant power assays were used to determine the antioxidant activities. Total phenolics were determined by Folin-Ciocalteu’s method. Antiglycation, α-glucosidase, and DPP-IV inhibitory assays were used to identify the antidiabetic activity. Cytotoxicity was investigated by the hemolytic activity of human red blood cells (RBCs). The leaf extract of Nauclea orientalis demonstrated the highest activity for DPPH inhibitory assay with an IC50 value of 29.57 ± 1.40 µg mL−1. The root extract of Acacia leucophloea was the most active in NO radical scavenging assay, α glucosidase inhibitory and antiglycation assay with IC50 value of 80.62 ± 5.36, 1.93 ± 0.08, and 4.63 ± 0.16 µg mL−1, respectively, and had the highest FRAP with an OD620 value of 0.38 ± 0.010. The Juniperus communis powder extract had the best activity for antiglycation and DPP-IV inhibitory assay with IC50 values of 4.50 ± 0.11 and 58.56 ± 4.03 µg mL−1, respectively. Phyllanthus reticulatus leaf extracts had the highest SO radical scavenging activity with IC50 values of 44.12 ± 4.75 µg mL−1. All extracts had relatively low cytotoxicity with a range of 9.13–17.92% lysis to RBCs.
{"title":"Multi-antidiabetic Properties and Cytotoxic Activities of Selected Indigenous Myanmar Medicinal Plants","authors":"T. S. Moe, M. Thida, H. Win, Khine Zar Wynn Myint, Zar Kyi Win, Nwe Nwe Htay","doi":"10.1080/10496475.2022.2084804","DOIUrl":"https://doi.org/10.1080/10496475.2022.2084804","url":null,"abstract":"ABSTRACT Antidiabetic and cytotoxic properties of selected indigenous Myanmar medicinal plants were assessed. In vitro scavenging assays of 2,2-diphenyl-1- picrylhydrazyl, nitric oxide, superoxide radicals, and ferric reducing antioxidant power assays were used to determine the antioxidant activities. Total phenolics were determined by Folin-Ciocalteu’s method. Antiglycation, α-glucosidase, and DPP-IV inhibitory assays were used to identify the antidiabetic activity. Cytotoxicity was investigated by the hemolytic activity of human red blood cells (RBCs). The leaf extract of Nauclea orientalis demonstrated the highest activity for DPPH inhibitory assay with an IC50 value of 29.57 ± 1.40 µg mL−1. The root extract of Acacia leucophloea was the most active in NO radical scavenging assay, α glucosidase inhibitory and antiglycation assay with IC50 value of 80.62 ± 5.36, 1.93 ± 0.08, and 4.63 ± 0.16 µg mL−1, respectively, and had the highest FRAP with an OD620 value of 0.38 ± 0.010. The Juniperus communis powder extract had the best activity for antiglycation and DPP-IV inhibitory assay with IC50 values of 4.50 ± 0.11 and 58.56 ± 4.03 µg mL−1, respectively. Phyllanthus reticulatus leaf extracts had the highest SO radical scavenging activity with IC50 values of 44.12 ± 4.75 µg mL−1. All extracts had relatively low cytotoxicity with a range of 9.13–17.92% lysis to RBCs.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41479046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-02DOI: 10.1080/10496475.2022.2084481
Srinivasa Rao Kareti, R. H. Haran, Vivek Singh Rajpoot
ABSTRACT Ethnobotanical data were collected from local traditional healers using semi-structured questionnaires, interviews, and group discussions in 29 villages of Anuppur district, Madhya Pradesh, Central India. A total of 114 plant species from 103 genera from 59 families were identified as being used in the treatment of different diseases. Trees constituted the highest proportion of 43 species (38%), and the most preferred method of preparation of traditional medicines from plant material was in crushed powdered form (68, 40.47%). The informants’ consensus about the usage of medicinal plants in the study area ranged from 0.18 to 0.5 for 13 categories. The fidelity level for 11 plant species ranged from 33.33% to 75%.
{"title":"Diversity of Ethnomedicinal Plants among Tribal Communities in Anuppur District, Madhya Pradesh, Central India","authors":"Srinivasa Rao Kareti, R. H. Haran, Vivek Singh Rajpoot","doi":"10.1080/10496475.2022.2084481","DOIUrl":"https://doi.org/10.1080/10496475.2022.2084481","url":null,"abstract":"ABSTRACT Ethnobotanical data were collected from local traditional healers using semi-structured questionnaires, interviews, and group discussions in 29 villages of Anuppur district, Madhya Pradesh, Central India. A total of 114 plant species from 103 genera from 59 families were identified as being used in the treatment of different diseases. Trees constituted the highest proportion of 43 species (38%), and the most preferred method of preparation of traditional medicines from plant material was in crushed powdered form (68, 40.47%). The informants’ consensus about the usage of medicinal plants in the study area ranged from 0.18 to 0.5 for 13 categories. The fidelity level for 11 plant species ranged from 33.33% to 75%.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46683407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/10496475.2022.2079793
Shashank Kumar, A. Pandey
ABSTRACT Pharmacological potential of serially extracted Solanum xanthocarpum fruit (SXF) extracts in terms of antioxidant, anticancer, and antibacterial activities was evaluated. Chemical characterization of the potent extract was done by HPLC, LC-MS-MS, and GC-MS techniques. In vitro antioxidant models, viz. hydroxyl radical scavenging, metal ion chelation, total antioxidant capacity, and ferric reducing antioxidant power, were used to assess the antioxidant potential of extract. Cytotoxicity of the SXF extracts was tested against prostate, ovary, and breast cancer cell lines (DU-145, IGR-OV-1, and MCF-7) using sulforhodamine (SRB) assay. The antibacterial potential was assessed against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia, and Escherichia coli bacteria. Results indicated potential pharmacological activities and the presence of pharmacologically active phytoconstituents in the SKF extracts.
{"title":"Pharmacological potential of serially extracted Solanum xanthocarpum fruit extracts and their phytochemical characterization","authors":"Shashank Kumar, A. Pandey","doi":"10.1080/10496475.2022.2079793","DOIUrl":"https://doi.org/10.1080/10496475.2022.2079793","url":null,"abstract":"ABSTRACT Pharmacological potential of serially extracted Solanum xanthocarpum fruit (SXF) extracts in terms of antioxidant, anticancer, and antibacterial activities was evaluated. Chemical characterization of the potent extract was done by HPLC, LC-MS-MS, and GC-MS techniques. In vitro antioxidant models, viz. hydroxyl radical scavenging, metal ion chelation, total antioxidant capacity, and ferric reducing antioxidant power, were used to assess the antioxidant potential of extract. Cytotoxicity of the SXF extracts was tested against prostate, ovary, and breast cancer cell lines (DU-145, IGR-OV-1, and MCF-7) using sulforhodamine (SRB) assay. The antibacterial potential was assessed against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia, and Escherichia coli bacteria. Results indicated potential pharmacological activities and the presence of pharmacologically active phytoconstituents in the SKF extracts.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47766920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/10496475.2022.2077878
R. Pervin, Tauhidur Rahman Nurrunabi, R. Biswas, M. Islam, K. Islam, D. I. Sharif, M. Billah, F. Sabrin
ABSTRACT Antioxidant, antibacterial, and brine shrimp lethality bioassay of Brownlowia tersa leaf and bark extracts were performed. The half maximal inhibitory concentration (IC50) values in DPPH free radical scavenging assay for the bark and leaf extracts of petroleum ether, chloroform, and methanol were 102.09 ± 0.53, 236.28 ± 0.85, 27.90 ± 0.56, and 183.74 ± 0.75, 45.78 ± 0.27, and 35.12 ± 0.55 µg mL−1, respectively. Total phenolic content was the higher in methanolic bark and leaf extracts than in other solvents. Total flavonoid content was the highest in the methanolic leaf extracts and the lowest in chloroform bark extract. Petroleum ether and methanolic extracts of bark and leaf demonstrated antibacterial activity against Escherichia coli ATCC 28739 and Pseudomonas aeruginosa ATCC 27833.
{"title":"Assessment of Antioxidant, Antibacterial, and Preliminary Cytotoxic Properties of Brownlowia Tersa","authors":"R. Pervin, Tauhidur Rahman Nurrunabi, R. Biswas, M. Islam, K. Islam, D. I. Sharif, M. Billah, F. Sabrin","doi":"10.1080/10496475.2022.2077878","DOIUrl":"https://doi.org/10.1080/10496475.2022.2077878","url":null,"abstract":"ABSTRACT Antioxidant, antibacterial, and brine shrimp lethality bioassay of Brownlowia tersa leaf and bark extracts were performed. The half maximal inhibitory concentration (IC50) values in DPPH free radical scavenging assay for the bark and leaf extracts of petroleum ether, chloroform, and methanol were 102.09 ± 0.53, 236.28 ± 0.85, 27.90 ± 0.56, and 183.74 ± 0.75, 45.78 ± 0.27, and 35.12 ± 0.55 µg mL−1, respectively. Total phenolic content was the higher in methanolic bark and leaf extracts than in other solvents. Total flavonoid content was the highest in the methanolic leaf extracts and the lowest in chloroform bark extract. Petroleum ether and methanolic extracts of bark and leaf demonstrated antibacterial activity against Escherichia coli ATCC 28739 and Pseudomonas aeruginosa ATCC 27833.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47886206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/10496475.2022.2078919
K. Khulbe, Poonam Takuli, Parikshit Kumar, Archana Parki
ABSTRACT Aerial parts of Polystichum squarrosum (Dryopteridaceae) collected from Kumaun Himalaya, India were assessed for antioxidant potential using the methanolic extract in vitro for DPPH free radical scavenging and ferric-reducing antioxidant assays. The DPPH assay exhibited potent free radical scavenging capacity (IC50, 6.03 ± 1.41 µg mL−1), as well as ferric-reducing antioxidant activity (886.2 mg AAE gm−1 dry extract). In the antibacterial activity tests, the methanolic extract inhibited the growth of Klebsiella pneumoniae (MIC 14 ± 0.57 mm) and Agrobacterium tumefaciens (MIC 12 ± 0.7 mm). Totally, 65 phytochemical compounds were identified using gas chromatography mass spectrometry (GC-MS). Total phenolics (913.33 ± 39.23 mg GAE g−1 dry extract) and total flavonoid contents (30.0 ± 2.64 mg QE g−1 dry extract) were estimated qualitatively. These results demonstrated antioxidant and antimicrobial activities due to the presence of bioactive phytochemicals.
{"title":"Phytochemical Analysis and in Vitro Assessment of Himalayan Polystichum squarrosum Extract for Its Antioxidant and Antimicrobial Activities","authors":"K. Khulbe, Poonam Takuli, Parikshit Kumar, Archana Parki","doi":"10.1080/10496475.2022.2078919","DOIUrl":"https://doi.org/10.1080/10496475.2022.2078919","url":null,"abstract":"ABSTRACT Aerial parts of Polystichum squarrosum (Dryopteridaceae) collected from Kumaun Himalaya, India were assessed for antioxidant potential using the methanolic extract in vitro for DPPH free radical scavenging and ferric-reducing antioxidant assays. The DPPH assay exhibited potent free radical scavenging capacity (IC50, 6.03 ± 1.41 µg mL−1), as well as ferric-reducing antioxidant activity (886.2 mg AAE gm−1 dry extract). In the antibacterial activity tests, the methanolic extract inhibited the growth of Klebsiella pneumoniae (MIC 14 ± 0.57 mm) and Agrobacterium tumefaciens (MIC 12 ± 0.7 mm). Totally, 65 phytochemical compounds were identified using gas chromatography mass spectrometry (GC-MS). Total phenolics (913.33 ± 39.23 mg GAE g−1 dry extract) and total flavonoid contents (30.0 ± 2.64 mg QE g−1 dry extract) were estimated qualitatively. These results demonstrated antioxidant and antimicrobial activities due to the presence of bioactive phytochemicals.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46008525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-18DOI: 10.1080/10496475.2022.2077497
M. Akande, F. Nwinyi, M. O. Egua, J. O. Ode, M. M. Onakpa, H. Mikail, S. Onoja, A. Mohammed, D. Akumka
ABSTRACT Erythrophleum guineense G. Don is an enormous shade species that is indigenous to moist regions of Africa. Various forms of its bark are used to treat heart diseases, edema, headache, and body pains. In this study, the stem bark of Erythrophleum guineense was subjected to double maceration and extracted with 80% methanol. The methanol extract was screened for its phytochemical components and in vitro antioxidant activity through the utilization of the Ferric Reducing/Antioxidant Power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity procedures. The acute toxicity of the plant extract was evaluated using Lorke’s method in albino rats. Screening was conducted in normoglycemic and glucose-challenged albino rats to determine the plant extract’s control of blood glucose levels. The doses of the methanol extract of Erythrophleum guineense tested in rats through the oral route were 100, 200, and 400 mg kg−1 body weights of the rats. The effects were compared with glibenclamide (0.2 mg kg−1 per os) and normal saline. The phytochemical constituents of the methanol extract of Erythrophleum guineense were saponins, terpenes, tannins, steroids, carbohydrates, and alkaloids. The results indicated that the plant extract possessed antioxidant and hypoglycemic properties. Further research is warranted to isolate the active hypoglycemic principle of the stem bark of Erythrophleum guineense.
{"title":"An Assessment of the Hypoglycemic and Antioxidant Properties of the Methanol Extract of Erythrophleum guineense Stem Bark in Albino Rats","authors":"M. Akande, F. Nwinyi, M. O. Egua, J. O. Ode, M. M. Onakpa, H. Mikail, S. Onoja, A. Mohammed, D. Akumka","doi":"10.1080/10496475.2022.2077497","DOIUrl":"https://doi.org/10.1080/10496475.2022.2077497","url":null,"abstract":"ABSTRACT Erythrophleum guineense G. Don is an enormous shade species that is indigenous to moist regions of Africa. Various forms of its bark are used to treat heart diseases, edema, headache, and body pains. In this study, the stem bark of Erythrophleum guineense was subjected to double maceration and extracted with 80% methanol. The methanol extract was screened for its phytochemical components and in vitro antioxidant activity through the utilization of the Ferric Reducing/Antioxidant Power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity procedures. The acute toxicity of the plant extract was evaluated using Lorke’s method in albino rats. Screening was conducted in normoglycemic and glucose-challenged albino rats to determine the plant extract’s control of blood glucose levels. The doses of the methanol extract of Erythrophleum guineense tested in rats through the oral route were 100, 200, and 400 mg kg−1 body weights of the rats. The effects were compared with glibenclamide (0.2 mg kg−1 per os) and normal saline. The phytochemical constituents of the methanol extract of Erythrophleum guineense were saponins, terpenes, tannins, steroids, carbohydrates, and alkaloids. The results indicated that the plant extract possessed antioxidant and hypoglycemic properties. Further research is warranted to isolate the active hypoglycemic principle of the stem bark of Erythrophleum guineense.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47730688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-13DOI: 10.1080/10496475.2022.2076004
R. K, Dennis Thomas T
ABSTRACT Micropropagation protocol for Diplocyclos palmatus through direct shoot induction from isolated cotyledon and leaf explants was established. Cotyledons were isolated from 2-week-old seedlings and cultured in the Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP, 0.5–2.0 mg L−1) alone or in combination with α-naphthalene acetic acid (NAA, 0.2–1.0 mg L−1) for shoot induction. The highest frequency of response (89%) and the mean number of shots (6.8) were observed in an MS medium supplemented with 1.5 mg L−1 BAP and 0.5 mg L−1 NAA. Leaves at various stages of development were isolated from in vitro grown shoots and cultured in an MS medium supplemented with different concentrations of BAP (0.5–2.5 mg L−1), Thidiazuron (TDZ, 0.5–2.5 mg L−1) alone and in combination with NAA (0.2 mg L−1) for shoot induction. The highest shoot induction frequency of 91% and shoot number of 7.8 shoots per explant were observed when 15-d-old- leaves were cultured in an MS medium enriched with 2.0 mg L−1 TDZ and 0.5 mg L−1 NAA after 45 d. The isolated cotyledon and leaf-derived shoots showed optimum rooting on ½ MS medium fortified with 4.0 mg L−1 indole-3-butyric acid (IBA). The rooted plants were transferred to the soil with 90% success.
{"title":"In Vitro Plant Regeneration via Adventitious Shoot Regeneration from Cotyledon and Leaf Explants of Diplocyclos Palmatus","authors":"R. K, Dennis Thomas T","doi":"10.1080/10496475.2022.2076004","DOIUrl":"https://doi.org/10.1080/10496475.2022.2076004","url":null,"abstract":"ABSTRACT Micropropagation protocol for Diplocyclos palmatus through direct shoot induction from isolated cotyledon and leaf explants was established. Cotyledons were isolated from 2-week-old seedlings and cultured in the Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP, 0.5–2.0 mg L−1) alone or in combination with α-naphthalene acetic acid (NAA, 0.2–1.0 mg L−1) for shoot induction. The highest frequency of response (89%) and the mean number of shots (6.8) were observed in an MS medium supplemented with 1.5 mg L−1 BAP and 0.5 mg L−1 NAA. Leaves at various stages of development were isolated from in vitro grown shoots and cultured in an MS medium supplemented with different concentrations of BAP (0.5–2.5 mg L−1), Thidiazuron (TDZ, 0.5–2.5 mg L−1) alone and in combination with NAA (0.2 mg L−1) for shoot induction. The highest shoot induction frequency of 91% and shoot number of 7.8 shoots per explant were observed when 15-d-old- leaves were cultured in an MS medium enriched with 2.0 mg L−1 TDZ and 0.5 mg L−1 NAA after 45 d. The isolated cotyledon and leaf-derived shoots showed optimum rooting on ½ MS medium fortified with 4.0 mg L−1 indole-3-butyric acid (IBA). The rooted plants were transferred to the soil with 90% success.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43506372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-03DOI: 10.1080/10496475.2022.2068723
Douae Taha, I. Bourais, S. El hajjaji, A. Bouyahya, H. Khamar, Naima Iba
ABSTRACT An ethnobotanical survey was conducted in Laayoune Boujdour Sakia El Hamra region to inventory medicinal plants of South of Morocco. 248 informants including 220 local inhabitants and 28 herbalists were interviewed. Data were analyzed using seven indices: Use Value (UV), botanical Family Use Value (FUV), Fidelity Level (FL), Rank Order Priority (ROP), Informant Consensus Factor (FIC) and Jaccard similarity Index (JI). The highest UV value was recorded for Acacia tortilis subsp. raddiana (UV = 0.44). Brassicaceae was the most frequently used by inhabitants of the region (FUV = 0.34). The highest FL was recorded for A. tortilis with a value of 100%. A. tortilis had the highest ROP with a value of 100% for treating gastrointestinal disorders, diabetes, and blood pressure. The highest FIC value was recorded for both hair loss and dandruff care and kidney disorder treatments (FIC = 1).
{"title":"Traditional Medicine Knowledge of Medicinal Plants Used in Laayoune Boujdour Sakia El Hamra Region, Morocco","authors":"Douae Taha, I. Bourais, S. El hajjaji, A. Bouyahya, H. Khamar, Naima Iba","doi":"10.1080/10496475.2022.2068723","DOIUrl":"https://doi.org/10.1080/10496475.2022.2068723","url":null,"abstract":"ABSTRACT An ethnobotanical survey was conducted in Laayoune Boujdour Sakia El Hamra region to inventory medicinal plants of South of Morocco. 248 informants including 220 local inhabitants and 28 herbalists were interviewed. Data were analyzed using seven indices: Use Value (UV), botanical Family Use Value (FUV), Fidelity Level (FL), Rank Order Priority (ROP), Informant Consensus Factor (FIC) and Jaccard similarity Index (JI). The highest UV value was recorded for Acacia tortilis subsp. raddiana (UV = 0.44). Brassicaceae was the most frequently used by inhabitants of the region (FUV = 0.34). The highest FL was recorded for A. tortilis with a value of 100%. A. tortilis had the highest ROP with a value of 100% for treating gastrointestinal disorders, diabetes, and blood pressure. The highest FIC value was recorded for both hair loss and dandruff care and kidney disorder treatments (FIC = 1).","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45716904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-15DOI: 10.1080/10496475.2022.2062633
Subhash Sharma, Prof Vikas Kumar, Chandrika Attri, A. Sourirajan, K. Dev
ABSTRACT A reliable protocol for in vitro propagation of Potentilla nepalensis was developed and the phytochemical, antioxidant, and antimicrobial effects of in vitro propagated and wild grown P. nepalensis plants were compared. Leaves of P. nepalensis used as explants were cultured on conventional Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. Optimum sterilization conditions were achieved by using 0.04% Bavistin for 10 min, 0.04% sodium hypochlorite for 1 min, and 0.04% mercuric chloride for 1 min and the optimum media composition for callus generation was MS minerals containing ammonium nitrate (165 mg L−1), calcium chloride (44 mg L−1), and growth regulators IBA (20 mg L−1), and BAP (4 mg L−1). The phenolic and flavonoid content were higher in wild grown plant (TPC-21.21 ± 0.54 mg g−1 GAE; TFC-4.24 ± 0.17 mg g−1RE) compared to in vitro propagated P. nepalensis (TPC-3.55889 ± 0.72 mg g−1 GAE; TFC-0.33 ± 0.07 mg g−1 RE). Although the antioxidant activity was high in wild grown plant (IC50-235 ± 92.120 µg mL−1) than in vitro propagated (IC50-682.989 ± 57.118 µg mL−1), the antimicrobial properties for bacterial and fungal pathogens were similar.
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