Pub Date : 2022-09-28DOI: 10.1080/10496475.2022.2128137
S. Pradhan, Vivek Sharma
ABSTRACT The phytoconstituent analyses have been undertaken for the first time from the different plant parts of Inula grandiflora Willd. collected from two habitats (open dry slopes and moist places) of Govind Pashu Vihar National Park and Wildlife Sanctuary, Uttarakhand, India. The total phenolic and flavonoid contents and antioxidant activity have been estimated from the methanolic extracts. Ten phenols and five flavonoids were quantified in the different parts using High Performance Liquid Chromatography-Photodiode Array Detection (HPLC-PDA) method. Among the plant parts, the trend of accumulation of total phenols and flavonoids was as follows: roots > flowers > stems > leaves in both populations. It has been observed that Kalkati Dhar germplasm has the highest phenolic content in the roots (16.42 ± 0.12 mg g−1 DW), while accession collected from Renugaad has the highest flavonoid content in the flowers (4.34 ± 0.024 mg g−1 DW). Eight bioactive compounds (caffeic acid, resorcinol, p-coumaric acid, ferulic acid, veratric acid, myristicin, coumarin, and quercetin) were found dominant in the Kalkati Dhar germplasm (3200 m asl, high altitude). Significantly, seven compounds, viz; vanillic acid, vanillin, cinnamic acid, epicatechin, catechol, luteolin, and rutin, were found predominate in the Renugaad germplasm (2750 m asl, low altitude). Overall, root part accumulates most of the bioactive polyphenolic compounds as compared to the aerial parts; therefore, it has the highest antioxidant potential.
{"title":"Polyphenols in Different Plant Parts of Inula grandiflora Collected from Two Habitats of Uttarakhand Himalayas","authors":"S. Pradhan, Vivek Sharma","doi":"10.1080/10496475.2022.2128137","DOIUrl":"https://doi.org/10.1080/10496475.2022.2128137","url":null,"abstract":"ABSTRACT The phytoconstituent analyses have been undertaken for the first time from the different plant parts of Inula grandiflora Willd. collected from two habitats (open dry slopes and moist places) of Govind Pashu Vihar National Park and Wildlife Sanctuary, Uttarakhand, India. The total phenolic and flavonoid contents and antioxidant activity have been estimated from the methanolic extracts. Ten phenols and five flavonoids were quantified in the different parts using High Performance Liquid Chromatography-Photodiode Array Detection (HPLC-PDA) method. Among the plant parts, the trend of accumulation of total phenols and flavonoids was as follows: roots > flowers > stems > leaves in both populations. It has been observed that Kalkati Dhar germplasm has the highest phenolic content in the roots (16.42 ± 0.12 mg g−1 DW), while accession collected from Renugaad has the highest flavonoid content in the flowers (4.34 ± 0.024 mg g−1 DW). Eight bioactive compounds (caffeic acid, resorcinol, p-coumaric acid, ferulic acid, veratric acid, myristicin, coumarin, and quercetin) were found dominant in the Kalkati Dhar germplasm (3200 m asl, high altitude). Significantly, seven compounds, viz; vanillic acid, vanillin, cinnamic acid, epicatechin, catechol, luteolin, and rutin, were found predominate in the Renugaad germplasm (2750 m asl, low altitude). Overall, root part accumulates most of the bioactive polyphenolic compounds as compared to the aerial parts; therefore, it has the highest antioxidant potential.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44705644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-16DOI: 10.1080/10496475.2022.2116623
A. Morajkar, Bharatbhushan Sharma, Kiran R Kharat
ABSTRACT The ameliorative effect of aqueous (PPAqExt) and alcohol (PPAlcExt) extracts of Pongamia pinnata on degenerative histopathological changes in diabetic rats were investigated. Rats were divided into six groups. 1. Control group (NC). 2. Diabetic control group (DC)3. PPAqExt (TD1) 4. PPAlcExt (TD2) 5. Standard Control group (SD) 6. Vehicle Control group (VC). Blood samples were collected for the detection of Glycosylated Hemoglobin (HbA1c) levels. The vital organs pancreas, liver, kidney, and testis were prepared for histopathological studies. Treatment with PPAlcExt produced a reduction in HbA1c levels (8.4%) compared to DC (13.63%). The histopathological microscopic examination of diabetic rats showed marked changes in all the vital organs ranging from mild to severe along with cell infiltration, degeneration, and necrosis. Treatment with both the extracts showed a reversal in structural and cellular integrity of the vital organs involved in the pathophysiology of diabetes mellitus.
{"title":"Ameliorative Effect of Pongamia Pinnata on Histopathology of Vital Organs Involved in the Alloxan Induced Diabetic Rats","authors":"A. Morajkar, Bharatbhushan Sharma, Kiran R Kharat","doi":"10.1080/10496475.2022.2116623","DOIUrl":"https://doi.org/10.1080/10496475.2022.2116623","url":null,"abstract":"ABSTRACT The ameliorative effect of aqueous (PPAqExt) and alcohol (PPAlcExt) extracts of Pongamia pinnata on degenerative histopathological changes in diabetic rats were investigated. Rats were divided into six groups. 1. Control group (NC). 2. Diabetic control group (DC)3. PPAqExt (TD1) 4. PPAlcExt (TD2) 5. Standard Control group (SD) 6. Vehicle Control group (VC). Blood samples were collected for the detection of Glycosylated Hemoglobin (HbA1c) levels. The vital organs pancreas, liver, kidney, and testis were prepared for histopathological studies. Treatment with PPAlcExt produced a reduction in HbA1c levels (8.4%) compared to DC (13.63%). The histopathological microscopic examination of diabetic rats showed marked changes in all the vital organs ranging from mild to severe along with cell infiltration, degeneration, and necrosis. Treatment with both the extracts showed a reversal in structural and cellular integrity of the vital organs involved in the pathophysiology of diabetes mellitus.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47883919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.1080/10496475.2022.2121351
Liliana Santamaría-Acevedo, M. M. Levy, L. Torres-Martínez, S. Marchant, Fernando Rondón-González, E. Stashenko, M. Levy, J. L. Fuentes
ABSTRACT Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.
{"title":"Genome-derived Microsatellite Markers in Lippia origanoides from Colombia","authors":"Liliana Santamaría-Acevedo, M. M. Levy, L. Torres-Martínez, S. Marchant, Fernando Rondón-González, E. Stashenko, M. Levy, J. L. Fuentes","doi":"10.1080/10496475.2022.2121351","DOIUrl":"https://doi.org/10.1080/10496475.2022.2121351","url":null,"abstract":"ABSTRACT Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42698003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.1080/10496475.2022.2116513
Anwesha Chatterjee, Mayukh Acherjee, B. Das, S. Chakraborty, Harshata Pal
ABSTRACT The metabolites present in the crude methanolic extract of Leucas biflora were identified and characterized to obtain new leads for compounds with antibacterial effects. The plant extract showed antibacterial effects against both the gram-positive and gram-negative bacteria in disc-diffusion assay. Gas chromatography-mass spectrometry (GC-MS) results revealed nine types of high and low molecular weight chemical entities with varying quantities in the extract. An in silico target-fishing approach identified two compounds with multi-target-directed activities. Two metabolites, 3-Oxo-18-Nor-ent-ros-4-ene-15.alpha.,16-acetonide and 4-Dehydroxy-N-(4,5-methylenedioxy-2-nitrobenzylidene)-tyramine, inhibited two promising bacterial targets, FabH and DNA gyrase B.
{"title":"Multi-target Inhibitory Potency of Active Metabolites Dictates the Antimicrobial Activity of Indigenous Medicinal Plant Leucas biflora: GC-MS Analysis, Biological Evaluations, and Molecular Docking Studies","authors":"Anwesha Chatterjee, Mayukh Acherjee, B. Das, S. Chakraborty, Harshata Pal","doi":"10.1080/10496475.2022.2116513","DOIUrl":"https://doi.org/10.1080/10496475.2022.2116513","url":null,"abstract":"ABSTRACT The metabolites present in the crude methanolic extract of Leucas biflora were identified and characterized to obtain new leads for compounds with antibacterial effects. The plant extract showed antibacterial effects against both the gram-positive and gram-negative bacteria in disc-diffusion assay. Gas chromatography-mass spectrometry (GC-MS) results revealed nine types of high and low molecular weight chemical entities with varying quantities in the extract. An in silico target-fishing approach identified two compounds with multi-target-directed activities. Two metabolites, 3-Oxo-18-Nor-ent-ros-4-ene-15.alpha.,16-acetonide and 4-Dehydroxy-N-(4,5-methylenedioxy-2-nitrobenzylidene)-tyramine, inhibited two promising bacterial targets, FabH and DNA gyrase B.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43829515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-13DOI: 10.1080/10496475.2022.2122101
A. O. Opajobi, U. Uzuegbu, C. Elu, P. Y. Toloyai, I. Igwenyi, I. Onyesom
ABSTRACT The antimalarial activity of different parts (leaf, fruit, stem, and root) of Phyllanthus amarus and their associated impact on surrogate markers of insulin sensitivity in experimental mice, having ascertained their toxicity (LD50) index were documented. Results showed that the plant parts were nontoxic (LD50 > 5,000 mg kg−1) and were antimalarial in a dose-dependent manner, with the ethanol leaf extract having the highest parasite suppression followed by the fruit, root, and stem extracts. However, the root extract had the most profound impact on insulin sensitivity compared to other parts, although all showed improvement, with insulin sensitivity/resistance indices, with QUICKI vs. HOMA-IR showing a strong positive correlation (r = 0.993), but QUICKI vs. HOMA-IR and HOMA-IR vs. 1/HOMA = IR having strong negative correlation (r = −0.963 and −0.927, respectively). So, QUICKI and 1/HOMA-IR are surrogate indices of insulin sensitivity and HOMA-IR and TyG index, insulin resistance surrogate markers.
{"title":"Antiplasmodial Activity of Phyllanthus amarus and Associated Impact on Surrogate Markers of Insulin Sensitivity and Resistance Indices","authors":"A. O. Opajobi, U. Uzuegbu, C. Elu, P. Y. Toloyai, I. Igwenyi, I. Onyesom","doi":"10.1080/10496475.2022.2122101","DOIUrl":"https://doi.org/10.1080/10496475.2022.2122101","url":null,"abstract":"ABSTRACT The antimalarial activity of different parts (leaf, fruit, stem, and root) of Phyllanthus amarus and their associated impact on surrogate markers of insulin sensitivity in experimental mice, having ascertained their toxicity (LD50) index were documented. Results showed that the plant parts were nontoxic (LD50 > 5,000 mg kg−1) and were antimalarial in a dose-dependent manner, with the ethanol leaf extract having the highest parasite suppression followed by the fruit, root, and stem extracts. However, the root extract had the most profound impact on insulin sensitivity compared to other parts, although all showed improvement, with insulin sensitivity/resistance indices, with QUICKI vs. HOMA-IR showing a strong positive correlation (r = 0.993), but QUICKI vs. HOMA-IR and HOMA-IR vs. 1/HOMA = IR having strong negative correlation (r = −0.963 and −0.927, respectively). So, QUICKI and 1/HOMA-IR are surrogate indices of insulin sensitivity and HOMA-IR and TyG index, insulin resistance surrogate markers.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48357371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-06DOI: 10.1080/10496475.2022.2110348
N. Perera, M. Ediriweera, S. Samarakoon, Ilakya Selvakumar, Devipriya Nagarajan, Vadivel Vellingiri, K. Tennekoon
ABSTRACT Mangifera indica L. is a popular and widely available plant. Mangifera zeylanica Hook.f. is a plant endemic to Sri Lanka. Both plants bare edible fruits and possess medicinal properties. In the present study, potential anticancer effects of M. zeylanica and M. indica leaves were investigated in lung cancer cells. Hexane, chloroform, ethyl acetate, and methanol extracts of leaves from both plants were obtained using sequential extraction. Chloroform extract of M. zeylanica leaves (MZC) and ethyl acetate extract of M. indica leaves (MIEA) inhibited the proliferation, migration, and colony formation. These extracts showed low cytotoxicity in MRC-5 normal lung fibroblast cells. Furthermore, DNA fragmentation analysis and ethidium bromide/acridine orange (AO/EB) staining showed that MZC and MIEA can induce apoptosis. Gene expression analysis by real-time PCR identified that MZC and MIEA can regulate the expression of apoptosis-associated genes p53, Bax, and survivin. In addition, treatment of both MZC and MIEA decreased histone deacetylase (HDAC) activity, indicating the HDAC inhibitory potentials of leaf extracts. Liquid chromatography-mass spectrometry (LC-MS) identified the presence of mangiferin, 6-geranylnaringenin, nodakenin, and pyrogallol in both leaf extracts as major phytochemicals. Overall, our findings indicate that M. zeylanica and M. indica leaf extracts exert anticancer activity by decreasing the proliferation of non-small lung cancer cells possibly through the inhibition of the activity of HDACs, a major class of enzymes implicated in epigenetic modifications. This investigation provides a rationale to isolate compounds with anticancer and HDAC inhibitory potentials from M. zeylanica and M. indica leaves.
{"title":"Mangifera zeylanica and Mangifera indica Leaf Extracts Exert Anticancer Effects Possibly by Inhibiting Histone Deacetylases","authors":"N. Perera, M. Ediriweera, S. Samarakoon, Ilakya Selvakumar, Devipriya Nagarajan, Vadivel Vellingiri, K. Tennekoon","doi":"10.1080/10496475.2022.2110348","DOIUrl":"https://doi.org/10.1080/10496475.2022.2110348","url":null,"abstract":"ABSTRACT Mangifera indica L. is a popular and widely available plant. Mangifera zeylanica Hook.f. is a plant endemic to Sri Lanka. Both plants bare edible fruits and possess medicinal properties. In the present study, potential anticancer effects of M. zeylanica and M. indica leaves were investigated in lung cancer cells. Hexane, chloroform, ethyl acetate, and methanol extracts of leaves from both plants were obtained using sequential extraction. Chloroform extract of M. zeylanica leaves (MZC) and ethyl acetate extract of M. indica leaves (MIEA) inhibited the proliferation, migration, and colony formation. These extracts showed low cytotoxicity in MRC-5 normal lung fibroblast cells. Furthermore, DNA fragmentation analysis and ethidium bromide/acridine orange (AO/EB) staining showed that MZC and MIEA can induce apoptosis. Gene expression analysis by real-time PCR identified that MZC and MIEA can regulate the expression of apoptosis-associated genes p53, Bax, and survivin. In addition, treatment of both MZC and MIEA decreased histone deacetylase (HDAC) activity, indicating the HDAC inhibitory potentials of leaf extracts. Liquid chromatography-mass spectrometry (LC-MS) identified the presence of mangiferin, 6-geranylnaringenin, nodakenin, and pyrogallol in both leaf extracts as major phytochemicals. Overall, our findings indicate that M. zeylanica and M. indica leaf extracts exert anticancer activity by decreasing the proliferation of non-small lung cancer cells possibly through the inhibition of the activity of HDACs, a major class of enzymes implicated in epigenetic modifications. This investigation provides a rationale to isolate compounds with anticancer and HDAC inhibitory potentials from M. zeylanica and M. indica leaves.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49206785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-04DOI: 10.1080/10496475.2022.2117253
A. Khan, Mukesh Kr. Singh, P. Lavhale, R. Kaushik
ABSTRACT Solvent extracts of Cinnamomum tamala (CT) leaves were screened for phytochemicals, and the flavonoid and phenolic contents were estimated. The two standard marker compounds quercetin and eugenol were also quantified using high-performance thin-layer chromatography (HPTLC). The total flavonoid and phenolic contents were determined using aluminum chloride and Folin–Ciocalteu colorimetric methods, respectively. The ethanolic extracts had the highest total flavonoid and phenolic contents with 141.31 ± 0.053 mg QE g−1 and 178.96 ± 0.62 mg GAE g−1, respectively. The HPTLC method was used to detect spots for quercetin and eugenol (Rf value of 0.63 and 0.59, respectively). The amounts of eugenol and quercetin were 452.6 µg and 705.6 µg in 100 mg of the ethanolic extract of CT leaves, respectively.
摘要对肉桂(Cinnamomum tamala, CT)叶溶剂提取物进行化学成分筛选，并对其类黄酮和酚类物质含量进行测定。采用高效薄层色谱法对槲皮素和丁香酚两种标准标记物进行定量分析。采用氯化铝比色法测定总黄酮和酚类含量，采用福林比色法测定酚类含量。乙醇提取物总黄酮含量最高，分别为141.31±0.053 mg QE g−1和178.96±0.62 mg GAE g−1。采用HPTLC法对槲皮素和丁香酚进行斑点检测(Rf值分别为0.63和0.59)。丁香酚含量为452.6µg，槲皮素含量为705.6µg。
{"title":"Phytochemical Screening and HPTLC Analysis of Bio-active Markers of Ethanol Extract of Indian Bay Leaves","authors":"A. Khan, Mukesh Kr. Singh, P. Lavhale, R. Kaushik","doi":"10.1080/10496475.2022.2117253","DOIUrl":"https://doi.org/10.1080/10496475.2022.2117253","url":null,"abstract":"ABSTRACT Solvent extracts of Cinnamomum tamala (CT) leaves were screened for phytochemicals, and the flavonoid and phenolic contents were estimated. The two standard marker compounds quercetin and eugenol were also quantified using high-performance thin-layer chromatography (HPTLC). The total flavonoid and phenolic contents were determined using aluminum chloride and Folin–Ciocalteu colorimetric methods, respectively. The ethanolic extracts had the highest total flavonoid and phenolic contents with 141.31 ± 0.053 mg QE g−1 and 178.96 ± 0.62 mg GAE g−1, respectively. The HPTLC method was used to detect spots for quercetin and eugenol (Rf value of 0.63 and 0.59, respectively). The amounts of eugenol and quercetin were 452.6 µg and 705.6 µg in 100 mg of the ethanolic extract of CT leaves, respectively.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48021822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-11DOI: 10.1080/10496475.2022.2111016
S. Rashid, Naveed Ahmad Pathan, Hammad Ahmad Jan, Lone Rafiya Majeed, Bisma Nisar
ABSTRACT The ethnic knowledge of alternative medicines of Tehsil Uri was documented for its diverse eco-edaphic and floral features. Data were collected through semi-structured questionnaire, face-to-face interviews, and group discussions. The 573 informants were selected through snow-ball sampling. For quantification of the data Relative Importance Index (RII), Relative Frequency Citation (RFC) and Use Value (UV) were used. A total of 97 medicinal plants were reported for their medicinal values. The informants of age 40 years or above were more knowledgeable. Most of the local people (73.08%) used medicinal plants to cure digestive problems. The dominant family was Asteraceae (N = 16) and the most dominant life form was herb (73 Sp.). Leaves (38 Sp.) were the most commonly used plant part and decoction (N = 22) was the most common mode of preparation. The highest RFC value was reported for Juglans regia (0.88); the highest RII value was obtained for Achillea millefolium (0.97), and Punica granatum had the highest UV of 0.100.
{"title":"Study of Perceptional Attitude of Resource Limited Uri Populace of District Baramullah Toward Traditional Medicinal Usage in the Kashmir Himalayas","authors":"S. Rashid, Naveed Ahmad Pathan, Hammad Ahmad Jan, Lone Rafiya Majeed, Bisma Nisar","doi":"10.1080/10496475.2022.2111016","DOIUrl":"https://doi.org/10.1080/10496475.2022.2111016","url":null,"abstract":"ABSTRACT The ethnic knowledge of alternative medicines of Tehsil Uri was documented for its diverse eco-edaphic and floral features. Data were collected through semi-structured questionnaire, face-to-face interviews, and group discussions. The 573 informants were selected through snow-ball sampling. For quantification of the data Relative Importance Index (RII), Relative Frequency Citation (RFC) and Use Value (UV) were used. A total of 97 medicinal plants were reported for their medicinal values. The informants of age 40 years or above were more knowledgeable. Most of the local people (73.08%) used medicinal plants to cure digestive problems. The dominant family was Asteraceae (N = 16) and the most dominant life form was herb (73 Sp.). Leaves (38 Sp.) were the most commonly used plant part and decoction (N = 22) was the most common mode of preparation. The highest RFC value was reported for Juglans regia (0.88); the highest RII value was obtained for Achillea millefolium (0.97), and Punica granatum had the highest UV of 0.100.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44781234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-26DOI: 10.1080/10496475.2022.2103765
Md. Moshiur Rahman Khan, Tasmina Ferdous Susmi, Ma Miah, Md. Abu Reza, Md. Sifat Rahi
ABSTRACT The phytochemical constituents, antioxidant, cytotoxicity and anti-cancer activities of Leucas indica bark extract in methanol were evaluated. Brine shrimp lethality assay was used to assess the cytotoxicity while antioxidant capability was determined by DPPH and H2O2 free radical scavenging assay. Cell growth inhibition was estimated through hemocytometric cell counting after 5 d of treatment using EAC cells in in vivo mice model. This inhibition was due to the induction of apoptosis observed through fluorescence microscopy. The extract showed anticancer activity against EAC cells with 58.27% cell growth inhibition at 100 mg kg−1 b.w. Morphological alterations identified demonstrated apoptosis induction supported by intracellular ROS generation and DNA fragmentation.
{"title":"Morphological Alteration and Intracellular ROS Generation Confirm Apoptosis Induction on EAC Cells by Leucas Indica Bark Extract","authors":"Md. Moshiur Rahman Khan, Tasmina Ferdous Susmi, Ma Miah, Md. Abu Reza, Md. Sifat Rahi","doi":"10.1080/10496475.2022.2103765","DOIUrl":"https://doi.org/10.1080/10496475.2022.2103765","url":null,"abstract":"ABSTRACT The phytochemical constituents, antioxidant, cytotoxicity and anti-cancer activities of Leucas indica bark extract in methanol were evaluated. Brine shrimp lethality assay was used to assess the cytotoxicity while antioxidant capability was determined by DPPH and H2O2 free radical scavenging assay. Cell growth inhibition was estimated through hemocytometric cell counting after 5 d of treatment using EAC cells in in vivo mice model. This inhibition was due to the induction of apoptosis observed through fluorescence microscopy. The extract showed anticancer activity against EAC cells with 58.27% cell growth inhibition at 100 mg kg−1 b.w. Morphological alterations identified demonstrated apoptosis induction supported by intracellular ROS generation and DNA fragmentation.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44738389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20DOI: 10.1080/10496475.2022.2091074
Angela Nnenna Ukwuani-Kwaja, Salau Habiba, I. Abubakar
ABSTRACT The herbal contraceptives used by the residents of Kebbi state were surveyed. Data on plants, parts of plants, and mode of preparation were collected from 400 respondents using questionnaires and oral interviews. Twenty medicinal plants were identified as herbal contraceptives used in Kebbi state. The most frequently mentioned plant was Momordica charantia (32.8%), followed by Piliostigma thonningii (27.3%) and Moringa oleifera (17.8%). A majority of respondents (50.37%) relied solely on herbal contraceptives, with leaves and seeds being the most used plant parts.
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